Improved thallus culture device

By designing a bacterial culture device with a detachable top cover and base, the problems of cumbersome operation and high risk of contamination in existing technologies have been solved, achieving the effects of simplified operation and improved culture efficiency.

CN224091849UActive Publication Date: 2026-04-07張瑞能
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-01-23
Publication Date
2026-04-07

AI Technical Summary

Technical Problem

Existing bacterial culture devices are cumbersome to operate when replenishing water, nutrient solution or air, and are prone to contamination, increasing the risk of infection and affecting culture stability and efficiency.

Method used

A bacterial culture device with a detachable top cover and base was designed, equipped with an air filter, a detachable sealing component and a porous carrier, combined with a detachable replenishment tube and a fixing plug, to achieve flexible operation and prevent contamination, while the nutrient layer provides a stable growth environment.

Benefits of technology

It simplifies the operation process, reduces the risk of infection, improves the stability and efficiency of culture, and enhances the control of the bacterial growth environment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides an improved thallus culture device and aims at solving the problems that a traditional culture device is tedious in operation and high in infection risk. The device comprises an upper cover, a porous carrier, a supplementing pipe and a base, the upper cover is provided with an air hole, a fungus planting hole and a liquid supplementing hole and is provided with an air filter and a detachable sealing piece, the porous carrier covers a nutrition layer and is provided with a supplementing hole, the supplementing pipe is connected with the liquid supplementing hole and the supplementing hole, the whole structure facilitates supplementing operation, and the pollution risk is reduced.
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Description

Technical Field

[0001] This application relates to a bacterial culture device, and more particularly to an improved bacterial culture device. Background Technology

[0002] In the prior art, there is a fungal culture device that can be used to cultivate Antrodia camphorata. The device has a sealing film on the top to achieve a sealing effect and provide a suitable environment for fungal growth.

[0003] However, the device has several inconveniences in actual use. For example, each time water, nutrient solution or air is added, the user has to tear off the sealing film to operate, and after the addition is completed, the residual adhesive on the sealing film must be thoroughly removed and a new sealing film must be applied. The operation steps are cumbersome and time-consuming.

[0004] Even worse, this device can usually only be used for a single cultivation of Antrodia camphorata. During the process of cleaning the residual adhesive from the sealing film, the inside of the device is easily contaminated, increasing the risk of contamination by other microorganisms, which in turn affects the success rate of cultivation and the quality of the microorganisms.

[0005] In summary, existing technologies for the design and use of bacterial culture devices suffer from drawbacks such as inconvenient operation and high risk of infection, which limit their application efficiency and economy, and also adversely affect the stability of bacterial culture and the convenience of actual use. Utility Model Content

[0006] The main purpose of this application is to solve the problem of high infection risk that may occur when replenishing water, nutrient solution or air each time existing fungal culture devices are used to cultivate Antrodia camphorata, as well as the problem of needing to clean the residual adhesive of the sealing film.

[0007] To achieve the above objectives, one embodiment of this application provides a bacterial culture device, comprising a top cover, a porous carrier, a replenishment tube, and a base. The top cover has at least one vent, one inoculation hole, and one replenishment hole. The vent is equipped with an air filter, and the inoculation hole has a detachable first closure. A replenishment hole is formed on the side of the porous carrier, and the surface of the porous carrier is covered with a nutrient layer. The replenishment tube has a first end and a second end at its two ends, the second end extending through the replenishment hole and the first end extending through the replenishment hole, and the first end has a detachable second closure. The base has an accommodating space for accommodating the porous carrier and the replenishment tube, and the base can be detachably closed with the top cover.

[0008] In another embodiment of this application, the top of the base is provided with a plurality of positioning pieces, and the top cover has a plurality of fasteners corresponding to the plurality of positioning pieces, which can be fastened and abutted against the corresponding plurality of positioning pieces.

[0009] In another embodiment of this application, the bottom of the top cover is provided with a groove, the groove is provided with a sealing ring, and the base has a flange corresponding to the position of the groove. When the top cover is fastened to the base, the flange presses against the sealing ring.

[0010] In another embodiment of this application, the replenishment hole is provided with a first fixing plug, and the first end of the replenishment tube can extend to the first fixing plug.

[0011] In another embodiment of this application, the supplementary hole is provided with a second fixing plug, and the second end of the supplementary tube can extend to the second fixing plug.

[0012] In another embodiment of this application, the surface of the second fixing plug is provided with a plurality of reinforcing threads.

[0013] In another embodiment of this application, the supplementary tube has a positioning groove on the surface adjacent to the second end, the positioning groove being used to indicate the insertion depth of the supplementary tube.

[0014] In another embodiment of this application, a carrier is provided on each side of the accommodating space of the base, and each carrier has a liquid tank.

[0015] In another embodiment of this application, the porous carrier has a circumferential surface and a plurality of micropores recessed into the circumferential surface, and the nutrient layer is formed on the circumferential surface and the walls of the plurality of micropores.

[0016] In another embodiment of this application, the porous carrier is provided with a plurality of implantation holes, the plurality of implantation holes being located at a position on the porous carrier that is relatively lower than the supplementary hole, and the supplementary hole is not connected to the plurality of implantation holes.

[0017] Through the above, this application achieves the goal of repeated use without the need to clean residual adhesive by using a detachable top cover and base, significantly improving ease of use. At the same time, the air filter installed through the vent, along with the inoculation and replenishment holes that can be sealed at any time, effectively prevents the invasion of external bacteria, greatly reduces the risk of infection, and ensures the stability and success rate of bacterial culture.

[0018] Furthermore, the porous carrier's permeable structure and nutrient layer provide a stable growth environment for the bacteria, further improving cultivation efficiency and quality. The preparation process of the porous carrier achieves a balance between high porosity and structural stability through strict control of raw material ratios and calcination techniques, enhancing nutrient transfer efficiency and providing better support for bacterial growth. Attached Figure Description

[0019] Figure 1 This is a three-dimensional schematic diagram of the bacterial culture device of this application.

[0020] Figure 2 This is a three-dimensional exploded view of the bacterial culture device of this application.

[0021] Figure 3 For along Figure 1 A schematic diagram of the cross-section taken from the AA section line.

[0022] Figure 4 for Figure 3 A magnified schematic diagram of part of the structure.

[0023] Figure 5 This is a schematic diagram of an embodiment of the present application in which air and nutrient solution are added to the Antrodia camphorata culture device.

[0024] Figure 6 This is a flowchart illustrating the preparation method of the camphor tree fermented fertilizer liquid in this application.

[0025] Figure 7 This is a flowchart of the firing method for the porous carrier of this application. Detailed Implementation

[0026] To make the above and other objects, features and advantages of this application more apparent and understandable, preferred embodiments of this application are described in detail below with reference to the accompanying drawings:

[0027] Please refer to Figures 1 to 7 A bacterial culture device 100 is disclosed, comprising a top cover 10, a porous carrier 20, a replenishment tube 30, and a base 40. The top cover 10 has at least one air hole 11, one inoculation hole 12, and one replenishment hole 13. The air hole 11 is provided with an air filter 50, and the inoculation hole 12 is provided with a detachable first closure 51. The porous carrier 20 has a replenishment hole 21 on its side, and the surface of the porous carrier 20 is covered with a nutrient layer 60. The replenishment tube 30 has a first end 31 and a second end 32 at its two ends, respectively. The second end 32 extends through the replenishment hole 21, and the first end 31 extends through the replenishment hole 13. The first end 31 is provided with a detachable second closure 52. The base 40 has an accommodating space 41 for accommodating the porous carrier 20 and the replenishment tube 30. The base 40 can be detachably closed with the top cover 10.

[0028] With the above structure, the vents 11 on the top cover 10 are equipped with air filters 50, which can effectively promote gas exchange and prevent external contaminants from entering, maintaining a stable culture environment. The inoculation well 12 and the liquid replenishment well 13 are respectively equipped with detachable first sealing members 51 and second sealing members 52, providing flexible and safe inoculation and liquid replenishment operations, reducing the risk of contamination. The nutrient layer 60 on the surface of the porous carrier 20 can improve culture efficiency, and its porous structure further promotes the transport of nutrients and gases.

[0029] Please continue to refer to Figures 1 to 4The top of the base 40 is provided with multiple positioning pieces 42, and the top cover 10 has multiple fasteners 14 corresponding to the multiple positioning pieces 42. The fasteners 14 can be fastened and locked onto the corresponding positioning pieces 42 to form a tight fixing structure, ensuring that the top cover 10 and the base 40 are firmly connected. Since the base 40 and the top cover 10 are detachable, they can be reused to cultivate a new batch of mycelium or mushrooms, eliminating the need to deal with residual glue and improving the convenience of use.

[0030] Please continue to refer to Figures 2 to 4 The bottom of the upper cover 10 is provided with a groove 15, and a sealing ring 53 is provided in the groove 15. The base 40 has a flange 43 corresponding to the position of the groove 15. When the upper cover 10 is fastened to the base 40, the flange 43 presses against the sealing ring 53, so that the sealing ring 53 is elastically deformed to enhance the airtightness, effectively blocking the internal and external environment of the bacterial culture device 100 and preventing external pollutants from entering.

[0031] Please continue to refer to Figure 2 and Figure 3 The replenishment hole 13 is provided with a first fixing plug 54, through which the first end 31 of the replenishment tube 30 can extend; the replenishment hole 21 is provided with a second fixing plug 55, through which the second end 32 of the replenishment tube 30 can extend. Both the first fixing plug 54 and the second fixing plug 55 can enhance the stability and firmness of the replenishment tube 30 in connection with the upper cover 10 and the base 40. In addition, the replenishment tube 30 has a positioning groove 33 on the surface adjacent to the second end 32, which is used to indicate the insertion depth of the replenishment tube 30; when the second end 32 of the replenishment tube 30 is inserted and extends to the second fixing plug 55, when the positioning groove 33 is aligned with the edge of the second fixing plug 55, it indicates that the insertion depth of the replenishment tube 30 has reached the predetermined position, and further insertion of the replenishment tube 30 should be stopped.

[0032] Please continue to refer to Figure 2 and Figure 3 Alternatively, multiple reinforcing threads 551 can be provided around the surface of the second fixed plug 55. The reinforcing threads 551 can improve the bonding strength and stability between the second fixed plug 55 and the porous carrier 20.

[0033] Please continue to refer to Figure 2 and Figure 5 The base 40 has a carrier 56 on each side of the accommodating space 41. Each carrier 56 has a liquid tank 561 for holding an inducing agent. Different types of inducing agents can be added as needed. Their function is to achieve specific effects by regulating the growth environment of fungi or mushrooms, such as stimulating the proliferation of fungi or mushrooms or limiting their excessive growth, so as to precisely control the cultivation process and improve cultivation efficiency.

[0034] Please continue to refer to Figure 2 and Figure 5The porous carrier 20 has a circumferential surface 22 and multiple micropores 23 recessed into the circumferential surface 22. The nutrient layer 60 is formed on the walls of the circumferential surface 22 and the multiple micropores 23, ensuring that the fungi or mushrooms can obtain nutrients evenly and sufficiently, promoting their growth and reproduction. At the same time, the micropores 23 provide good channels for the flow of gas, liquid and nutrients, and significantly increase the effective contact area, improving the cultivation efficiency.

[0035] Please continue to refer to Figure 2 and Figure 5 The porous carrier 20 has multiple planting holes 24, which are located below the supplementary holes 21 and are not connected to each other. The planting holes 24 increase the surface area for fungi or mushrooms to attach and grow, thus increasing the yield per batch. Furthermore, the non-connection between the planting holes 24 and the supplementary holes 21 prevents the culture medium from flowing directly into the planting holes 24, reducing nutrient competition and maintaining favorable growth conditions for the fungi or mushrooms. Since the supplementary holes 21 are located above the planting holes 24, nutrients are naturally distributed into the porous carrier 20 under gravity, ensuring that the fungi or mushrooms can absorb nutrients evenly.

[0036] The nutrient layer 60 includes 40% to 60% of the total weight of the nutrient layer 60, 5% to 15% of the total weight of the nutrient layer 60, 5% to 10% of the total weight of the nutrient layer 60, 0.1% to 1% of the total weight of the nutrient layer 60, 30% to 50% of the total weight of the nutrient layer 60, and 10 mL to 20 mL of camphor tree fermented fertilizer solution added annually.

[0037] The fermentable carbon source is selected from one or a combination of glucose, molasses, or brown sugar. The fermentable carbon source may be 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60% of the total weight of the nutrient layer 60.

[0038] The nutritional supplement is selected from one or a combination of malt extract or yeast. The nutritional supplement may be 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9% of the total weight of the nutrient layer 60. %, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 10.1%, 10.2%, 10.3%, 10.4%, 10.5%, 10.6%, 10.7%, 10.8%, 10.9%, 11%, 11.1%, 11.2%, 11.3%, 11.4%, 11.5%, 11.6%, 11.7%, 11.8%, 11.9%, 12%, 12. 1%, 12.2%, 12.3%, 12.4%, 12.5%, 12.6%, 12.7%, 12.8%, 12.9%, 13%, 13.1%, 13.2%, 13.3%, 13.4%, 13.5%, 13.6%, 13.7%, 13.8%, 13.9%, 14%, 14.1%, 14.2%, 14.3%, 14.4%, 14.5%, 14.6%, 14.7%, 14.8%, 14.9%, or 15%.

[0039] The structural reinforcing agent is selected from one or a combination of calcium lignosulfonate or chitin. The structural reinforcing agent may be 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, or 10% of the total weight of the nutrient layer 60.

[0040] The thickener is selected from egg white gel. The thickener may be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1% of the total weight of the nutrient layer 60.

[0041] Water can be 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, or 50% of the total weight of the nutrient layer 60.

[0042] The fermented fertilizer solution of camphor tree can be prepared in the following quantities: 10 mL, 10.5 mL, 11 mL, 11.5 mL, 12 mL, 12.5 mL, 13 mL, 13.5 mL, 14 mL, 14.5 mL, 15 mL, 15.5 mL, 16 mL, 16.5 mL, 17 mL, 17.5 mL, 18 mL, 18.5 mL, 19 mL, 19.5 mL, or 20 mL.

[0043] Through the above structure, a fermentable carbon source provides the main energy source for the microorganisms, promoting their growth and metabolism. Nutrient supplements provide the microorganisms with the necessary trace elements and vitamins, ensuring a balance of physiological functions. A structural enhancer strengthens the mechanical strength and stability of the nutrient layer 60, preventing damage during use. A thickener enhances the viscosity and uniformity of the nutrient layer 60, preventing component separation or sedimentation. Water provides appropriate humidity and a dissolving medium to support the physiological needs of the microorganisms. Furthermore, the annual addition of fermented camphor tree fertilizer, made from pure camphor tree branches and leaves using a liquid fertilizer fermentation process, contains natural active ingredients that further promote the activity and growth efficiency of the microorganisms. The synergistic effect of these components ensures the stability and efficiency of the nutrient layer 60, providing optimized conditions for microbial cultivation.

[0044] Please refer to Figure 6 and paired Figures 1 to 5 The present application discloses a method for preparing a fermented fertilizer solution from camphor trees, comprising the following steps:

[0045] Step S1: Add the branches and leaves of the camphor tree to water and sugars in a ratio of 10:1 to 20:1 to form fermentation raw materials. The water-to-sugar ratio can be 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, or 20:1.

[0046] Step S2: Add fermentation broth to the fermentation raw materials, maintain a fermentation temperature of 30°C to 40°C, control the pH value between 5.5 and 7.0, and stir and aerate regularly. The fermentation broth is one or a combination of lactic acid bacteria, yeast, and Bacillus subtilis. The pH value can be 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, or 7.0.

[0047] Step S3: Ferment for up to 6 months, then filter to obtain camphor tree liquid fertilizer solution.

[0048] Please refer to Figure 7 and paired Figures 1 to 5 The method for firing a porous carrier 20 according to this application includes the following steps:

[0049] Step P1, Ingredient Preparation and Slurry Preparation: Aluminum oxide (Al₂O₃), silicon dioxide (SiO₂), and ferric oxide (Fe₂O₃) are mixed in a ratio approximately 50:40:1. Inorganic additives to enhance sintering properties, fluxes, binders, and a foaming agent to create pores during the firing process are added. Water is then added to prepare a slurry, which is ground to the required fineness and homogenized before use. The inorganic additives are one or a combination of calcium oxide (CaO), magnesium oxide (MgO), or titanium dioxide (TiO₂). The flux is one or a combination of potassium oxide (K₂O) or sodium oxide (Na₂O). The binder is one or a combination of clay, bentonite, kaolin, polyvinyl alcohol (PVA), or silicate (Na₂SiO₃). The foaming agent is one or a combination of calcium carbonate (CaCO₃), sodium bicarbonate (NaHCO₃), urea, or barium carbonate (BaCO₃).

[0050] Step P2, Granulation: The prepared slurry is dried and sieved using an atomizing dryer to form powder material with a size of 10 to 150 micrometers (µm). The powder size is 10 micrometers, 20 micrometers, 30 micrometers, 40 micrometers, 50 micrometers, 60 micrometers, 70 micrometers, 80 micrometers, 90 micrometers, 100 micrometers, 110 micrometers, 120 micrometers, 130 micrometers, 140 micrometers, or 150 micrometers.

[0051] Step P3, Aging Treatment: The prepared powder material is placed in an environment with controlled temperature and humidity to ensure uniform distribution of moisture and stress within the material. The aging temperature range is between 20℃ and 40℃, and the specific temperatures can be 20℃, 21℃, 22℃, 23℃, 24℃, 25℃, 26℃, 27℃, 28℃, 29℃, 30℃, 31℃, 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, 39℃, or 40℃. The aging humidity range is between 50% and 75% relative humidity (RH), and the specific humidity values ​​can be 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, or 75%.

[0052] Step P4, Heat Treatment: The aged powder material is laid in a high-temperature resistant carrier and subjected to heat treatment at a high temperature of 1200°C to 1400°C for at least 8 hours, during which the foaming agent decomposes and releases gas to form a porous structure. After the above-mentioned high-temperature treatment at 1200°C to 1400°C, chemical pollution and heavy metal pollution will be reduced to extremely low levels or even disappear. The high temperature can be 1200°C, 1210°C, 1220°C, 1230°C, 1240°C, 1250°C, 1260°C, 1270°C, 1280°C, 1290°C, 1300°C, 1310°C, 1320°C, 1330°C, 1340°C, 1350°C, 1360°C, 1370°C, 1380°C, 1390°C or 1400°C.

[0053] Step P5, post-cooling processing: The fired porous carrier 20 is cooled at room temperature and then cut, surface treated or processed as needed to achieve the required specifications.

[0054] With the above structure, the advantages of this application are:

[0055] 1. Stable environment for mycelial culture: After the top cover 10 is combined with the base 40, the gas input will be filtered through the air filter 50. The mycelial planting hole 12 and the liquid replenishment hole 13 are respectively equipped with a detachable first sealing part 51 and a second sealing part 52, which will be sealed after mycelial planting or liquid replenishment, effectively preventing external pollutants from entering and providing healthy growth conditions for fungi or mushrooms.

[0056] 2. Enhanced culture efficiency and yield: The combination of the porous carrier 20 structure and the nutrient layer 60 provides a sufficient and uniform nutrient supply; the design of planting holes 24 and micropores 23 further increases the attachment and growth area of ​​the bacteria, significantly improving the culture efficiency and yield of a single batch.

[0057] 3. Convenient for repeated use: The top cover 10 and the base 40 are detachable, eliminating the need to tear off the seal or deal with residual adhesive. The top cover 10 and the base 40 can be airtightly connected by a snap-fit ​​mechanism.

[0058] Finally, it should be understood that the embodiments described in this application are merely illustrative of the principles of the embodiments of this application. Other variations may also fall within the scope of this application. Therefore, alternative configurations of the embodiments of this application are considered as examples and not limitations, and are regarded as consistent with the teachings of this application. Accordingly, the embodiments of this application are not limited to the embodiments explicitly described and illustrated in this application.

[0059] Symbol Explanation

[0060] 100: Bacterial culture device.

[0061] 10: Top cover.

[0062] 11: Stomata.

[0063] 12:Plant hole.

[0064] 13: Liquid replenishment hole.

[0065] 14: Cutout.

[0066] 15: Groove.

[0067] 20: Porous carrier.

[0068] 21: Supplementary hole.

[0069] 22: Zhoumian.

[0070] 23: Micropores.

[0071] 24: Planting hole.

[0072] 30: Replenishment tube.

[0073] 31: First end.

[0074] 32: Second end.

[0075] 33: Positioning groove.

[0076] 40: Base.

[0077] 41: Storage space.

[0078] 42: Positioning plate.

[0079] 43: Flange.

[0080] 50: Air filter.

[0081] 51: First closure element.

[0082] 52: Second closure.

[0083] 53: Sealing ring.

[0084] 54: First fixed plug.

[0085] 55: Second fixing plug.

[0086] 551: Reinforced thread.

[0087] 56: Seat.

[0088] 561: Liquid tank.

[0089] 60: Nutrient layer.

[0090] S1~S3: Steps.

[0091] P1~P5: Steps.

Claims

1. An improved bacterial culture device, characterized in that: A top cover is provided with at least one air hole, one inoculation hole and one liquid replenishment hole. The air hole is equipped with an air filter and the inoculation hole is equipped with a detachable first closure. A porous carrier with a supplementary hole on its side, and a nutrient layer covering the surface of the porous carrier; A replenishment tube has a first end and a second end at its two ends, the second end extending through the replenishment hole and the first end extending through the replenishment hole, and the first end is provided with a detachable second closure. A base having a receiving space for accommodating the porous carrier and the supplementary tube, the base being detachably closed with the top cover.

2. The bacterial culture device as described in claim 1, characterized in that, The top of the base is provided with a plurality of positioning pieces, and the top cover has a plurality of fasteners corresponding to the plurality of positioning pieces, which can be fastened and abutted against the corresponding plurality of positioning pieces.

3. The bacterial culture device as described in claim 1, characterized in that, The bottom of the upper cover is provided with a groove, and a sealing ring is provided in the groove. The base has a flange corresponding to the position of the groove. When the upper cover is fastened to the base, the flange presses against the sealing ring.

4. The bacterial culture device as described in claim 1, characterized in that, The replenishment hole is provided with a first fixing plug, and the first end of the replenishment tube can extend to the first fixing plug.

5. The bacterial culture device as described in claim 1, characterized in that, The replenishment hole is provided with a second fixing plug, and the second end of the replenishment tube can extend into the second fixing plug.

6. The bacterial culture device as described in claim 5, characterized in that, The surface of the second fixing plug is provided with multiple reinforcing threads.

7. The bacterial culture device as described in claim 1, characterized in that, The supplementary tube has a positioning groove on the surface adjacent to the second end, the positioning groove being used to indicate the insertion depth of the supplementary tube.

8. The bacterial culture device as described in claim 1, characterized in that, A carrier is provided on each side of the accommodating space of the base, and each carrier has a liquid tank.

9. The bacterial culture device as described in claim 1, characterized in that, The porous carrier has a circumferential surface and a plurality of micropores recessed into the circumferential surface, and the nutrient layer is formed on the circumferential surface and the walls of the plurality of micropores.

10. The bacterial culture apparatus as described in claim 9, characterized in that, The porous carrier has multiple planting holes, which are located at a position lower than the supplementary holes on the porous carrier, and the supplementary holes are not connected to the multiple planting holes.