Kit for staining microorganisms

Abstract

The kit comprises a kit body and a base, and an inner cavity of the kit body is divided into a dye liquid cavity, a decolorizing liquid cavity, a washing liquid cavity and a waste liquid cavity; a liquid inlet is formed in the top of the waste liquid cavity, flow guide openings protruding downwards are formed in the bottoms of the dye liquid cavity, the decoloring liquid cavity and the washing liquid cavity, and liquid sealing structures are arranged at the ends of the flow guide openings; liquid storage tanks corresponding to the flow guide ports in position are arranged in the base, a bearing seat is arranged at the top of each liquid storage tank, a piercing flow guide column protruding upwards is arranged in each bearing seat and is of a hollow structure, an inlet is formed in the side wall of each piercing flow guide column, and an outlet is formed in the bottom surface of each bearing seat; and a liquid guide joint is arranged at the bottom of the liquid storage tank. The kit for microbial staining, provided by the utility model, is ingenious in structure, convenient to use and pollution-free, can simplify the staining operation process and improve the staining efficiency, and is convenient to replace a gram staining solution container, so that the kit is matched with full-automatic detection equipment for use.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical examination equipment technical field especially is related to a reagent kit for microbial staining. BACKGROUND

[0002] Gram staining method is a commonly used detection means in the aspect of microbial bacteria identification, through the staining method, bacteria can be identified into two categories of gram-positive bacteria which is stained purple and gram-negative bacteria which is stained red. Gram staining method mainly includes four steps of initial staining of gentian violet dye solution, iodine solution medium dyeing, decolorizing solution decoloring and safflower solution re-dyeing, and water washing is carried out after each step of dyeing and decoloring, these reagents are mostly packaged separately, and it is easy to confuse during use, and after dyeing by hand, the liquid in the bottle is easy to overflow due to the influence of hand temperature on the dye solution bottle; in addition, dyeing and water washing also need to consider waste liquid recovery, improper handling will cause the pollution of operation experiment table, which is difficult to clean, the recovered waste liquid is often directly poured into the sewer, which will cause the pollution of water tank and sewer, and the aerosol produced by volatilization has a large odor, which affects the health of experimenters.

[0003] In order to solve the above problems, some manufacturers have developed an automatic gram staining instrument, which solves some of the problems of manual dyeing, but since the dyeing reagents are still packaged separately, the positions of each dyeing liquid on the instrument are fixed, and one bottle needs to be placed when using, which will be placed in the wrong dyeing liquid if not careful, and most of them do not have matching washing liquid, which needs to be prepared additionally when using, and the operation is not convenient, in addition, the waste liquid generated by dyeing is still directly discharged into the sewer, causing pollution. More importantly, when the existing automatic gram staining instrument is used with the full-automatic microbial bacteria detection equipment, it cannot change the dyeing liquid without stopping, which will affect the normal working rhythm of the full-automatic detection equipment. SUMMARY

[0004] In order to solve the problems of dyeing liquid confusion, pollution of test table, no matching washing liquid, no waste liquid recovery and the like in the above dyeing process, the utility model provides a reagent kit for microbial staining which can realize non-stop replacement, and the following technical solutions can be adopted specifically:

[0005] The utility model discloses a kit for microbial dyeing, including box body and with its matched setting base, the inner chamber of box body is divided into dyeing liquid chamber, decolorizing liquid chamber, washing liquid chamber and waste liquid chamber, the top of waste liquid chamber is provided with liquid inlet, the bottom of dyeing liquid chamber, decolorizing liquid chamber, washing liquid chamber all is provided with the downward protruding flow guide port of the liquid chamber, the end of flow guide port is provided with liquid seal structure, be provided with the liquid pool of corresponding with each flow guide port position in base, the top of liquid pool is provided with the support seat for accommodating flow guide port, the bottom center of support seat is provided with the upward protruding puncture flow guide column, the puncture flow guide column is hollow structure, and its import setting is on the lateral wall of puncture flow guide column, and the export setting is on the bottom surface of support seat, the bottom of liquid pool is provided with the liquid guide connector of extending to the base outside, the liquid guide connector is linked with waste liquid chamber through infusion pipeline.

[0006] Preferably, the box body of the dyeing liquid chamber, the decolorizing liquid chamber and the washing liquid chamber is connected by a first sealing cover, the box body of the waste liquid chamber is connected by a second sealing cover, and the box body corresponding to the first sealing cover is higher than the box body corresponding to the second sealing cover. The stepped box body design can effectively prevent waste liquid from splashing and is conducive to waste liquid collection.

[0007] Preferably, the liquid inlet is arranged on the second sealing cover, and the second sealing cover is a concave surface structure gradually decreasing from the periphery to the liquid inlet. The above-mentioned concave surface structure is convenient for gathering and collecting the waste liquid generated during dyeing.

[0008] Preferably, a liquid suction pillow is arranged in the waste liquid chamber. The liquid suction pillow can quickly absorb the waste liquid, so that no waste liquid is discharged, reducing pollution and the generation of harmful aerosols.

[0009] Preferably, a handle is arranged on the side wall of the box body. The handle can be conveniently gripped for taking and placing, avoiding the influence of hand temperature on the reagents in the box body.

[0010] Preferably, the liquid seal structure comprises a hard bottle cap screwed on the outside of the flow guide port, a through hole corresponding to the position of the puncture flow guide column is arranged at the center of the hard bottle cap, a silica gel gasket is arranged on the inside of the hard bottle cap, and a thermoplastic sealing film is arranged on the outside of the hard bottle cap.

[0011] The above-mentioned thermoplastic sealing film can avoid the bottle cap from being contaminated by dust and other pollutants during long-term storage of the liquid storage box, and the silica gel gasket can seal the reagents in the cavity. When it is punctured, it can quickly recover to its original state, avoiding the residual solution from being splashed to the experiment table or the ground when replacing the container.

[0012] Preferably, a locking card is arranged on the outer wall of the support seat, and a screwing card seat matched with the locking card is arranged on the inner wall of the liquid pool. The above-mentioned clamping structure can firmly connect the support seat on the liquid pool, preventing the support seat from accidentally falling out, and facilitating replacement of the support seat.

[0013] Preferably, the top of the piercing guide column is provided with a piercing tip. The piercing tip can quickly pierce the liquid seal structure at the guide port, connecting the liquid storage chamber of the box to the liquid storage tank of the base, allowing the reagent to directly reach the liquid storage tank and increasing the liquid flow rate.

[0014] The microbial staining kit provided by this invention uses a single container that integrates all the necessary staining solutions (including staining solution, destaining solution, and washing solution), eliminating the need for individual preparation, saving time and reducing the chance of errors. Simultaneously, the container also integrates a waste liquid chamber, which seamlessly collects the waste liquid after staining, preventing contamination of the lab bench and achieving zero waste liquid discharge. The matching sizes of the staining solution chamber, destaining solution chamber, washing solution chamber, and waste liquid chamber prevent uneven distribution and waste of volume and solution. Furthermore, when changing staining reagents, the waste liquid container can be replaced along with the staining reagent. This design improves operational convenience. The dyeing solution, decolorizing solution, and washing solution are all sealed before use. When in use, the box and base are matched, and each reagent chamber is installed on the support through the bottom guide port. The liquid inside the chamber is connected to the storage tank by piercing the guide column, thus realizing the installation and replacement of the dyeing solution box without opening the lid, improving operational convenience, and not affecting the normal working cycle of the fully automatic detection equipment. The liquid guide connector at the bottom of the storage tank facilitates the insertion of a flexible tube, which can guide the dyeing solution, decolorizing solution, and washing solution to the designated location for Gram staining, without being limited by the staining space.

[0015] The microbial staining kit provided by this invention has a clever structure, is easy to use, and is pollution-free. It can simplify the staining operation process, improve staining efficiency, and facilitate the replacement of Gram staining solution containers, thus enabling it to be used with fully automated detection equipment. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model.

[0017] Figure 2 yes Figure 1 A schematic diagram of the exploded structure of the middle box.

[0018] Figure 3 yes Figure 1 A schematic diagram of the structure of the central base.

[0019] Figure 4 yes Figure 3 A three-dimensional perspective view of the intermediate liquid storage tank.

[0020] Figure 5 yes Figure 3 A three-dimensional perspective view of the central support. Detailed Implementation

[0021] The embodiments of the present utility model will be described in detail below with reference to the accompanying drawings. These embodiments are implemented on the premise of the technical solution of the present utility model, and detailed implementation manners and specific working processes are given. However, the protection scope of the present utility model is not limited to the following embodiments.

[0022] As Figures 1-3 shown, the kit for microbial staining described in the present utility model includes a box body 1 and a base 2 that is configured to match it. Both ends of the box body 1 are designed with an arc shape. A handle 18 is installed on the side wall of one end, which is convenient for grasping and taking and placing, and can avoid the influence of hand temperature on the reagents in the box body 1. The inner cavity of the box body 1 is divided into a dye solution chamber 11, a decolorizing solution chamber 12, a washing solution chamber 13 and a waste liquid chamber 14. In this embodiment, there is one washing solution chamber 13 and one waste liquid chamber 14 respectively, and their volumes are relatively large, and they are respectively arranged at both ends of the box body 1; the dye solution chamber 11 and the decolorizing solution chamber 12 are arranged in a row in the middle of the box body 1. Among them, there are three dye solution chambers 11 and one decolorizing solution chamber 12. The sizes of the above-mentioned dye solution chamber 11, decolorizing solution chamber 12, washing solution chamber 13 and waste liquid chamber 14 are configured according to the required amount for staining operations, so as to avoid volume and solution waste caused by uneven distribution.

[0023] In order to prevent waste liquid from splashing and facilitate waste liquid collection, the box body 1 in this embodiment adopts a stepped design, that is, the box body heights of the dye solution chamber 11, decolorizing solution chamber 12 and washing solution chamber 13 are the same, and they are connected by a first sealing cover. The box body of the waste liquid chamber 14 is connected by a second sealing cover, and the box body corresponding to the first sealing cover is higher than the box body corresponding to the second sealing cover. A mother groove is provided around the top of the box body of each cavity, and a male groove is correspondingly provided on the sealing cover. The male and female grooves are used in combination with a colloid to effectively seal each chamber, so that they form separate spaces and do not interfere with each other. The first sealing cover is a closed plate, and the second sealing cover is a concave structure that gradually decreases from the periphery to the center, and a liquid inlet 15 is provided at the lowest point of the concave surface, which is convenient for gathering and collecting the waste liquid generated by staining. Further, a liquid absorption pillow 16 is provided in the waste liquid chamber 14. The liquid absorption pillow 16 can quickly absorb the waste liquid, so that there is no waste liquid discharged, reducing pollution and the generation of harmful aerosols.

[0024] The bottom of each of the dyeing chamber 11, decolorizing chamber 12, and washing chamber 13 is provided with a downwardly protruding guide port 17, and the end of the guide port 17 is provided with a liquid seal structure. In this embodiment, the liquid seal structure includes a rigid bottle cap 31 screwed onto the outside of the guide port 17. The rigid bottle cap 31 has a through hole in its center, a silicone gasket 32 ​​is provided on the inner side of the rigid bottle cap 31, and a thermoplastic sealing film is provided on the outer side of the rigid bottle cap 31. When dispensing reagents, the box 1 is inverted, and the corresponding reagent is injected into each chamber through the guide port 17. Then, the silicone gasket 32 ​​and the rigid bottle cap 31 are installed for sealing. To ensure that the rigid bottle cap 31 is not contaminated, its outer surface can be treated with a thermoplastic sealing film. The aforementioned silicone gasket 32 ​​can seal the reagent in the chamber. When it is punctured, it can quickly restore its original shape, preventing residual solution from dripping onto the lab bench or floor when changing containers.

[0025] The base 2 contains a liquid storage tank 3 corresponding to the positions of each flow port 17. The top of the liquid storage tank 3 is provided with a support 4 to accommodate the flow ports 17. A locking clip 51 is provided on the outer wall of the support 4, and a matching screw-on locking seat 52 is provided on the inner wall of the liquid storage tank 3. Both the locking clip 51 and the screw-on locking seat 52 are L-shaped. By aligning the locking clip 51 with the upper opening of the screw-on locking seat 52 and pressing it down, and then rotating it along the horizontal slot of the screw-on locking seat 52 to the end of the slot, the support 4 can be securely connected to the liquid storage tank 3, preventing accidental dislodgement. Furthermore, this movable locking structure facilitates the replacement of the support 4.

[0026] A piercing guide column 6 protruding upwards is provided at the center of the bottom of the support 4 (corresponding to the central through hole of the hard bottle cap 31). A piercing tip is provided at the top of the piercing guide column 6, and a hollow flow channel is located below the piercing tip. The inlet of the flow channel is located on the side wall of the piercing guide column 6, and the outlet is located on the bottom surface of the support 4. After the box 1 is installed on the base 2, the guide port 17 is inserted into the support 4. At this time, the piercing tip of the piercing guide column 6 quickly pierces the silicone gasket 32 ​​at the end of the guide port 17. The liquid enters the hollow flow channel through the inlet on the side wall of the piercing guide column 6 and flows into the storage tank 3 through the outlet at the bottom.

[0027] The bottom of the aforementioned liquid storage tank 3 is provided with a liquid guide connector 7 extending beyond the base 1. The liquid guide connector 7 can be connected to the waste liquid chamber 14 through a liquid delivery pipe connected to a driver.

[0028] During the staining process, first connect one end of the flexible tube with the actuator to each of the liquid guide connectors 7, then comb the tube and arrange it neatly on both sides of the base 2, and fix the other end of the tube above the top cover of the waste liquid chamber 14; then peel off the thermoplastic seal film outside the flow guide port 17 of each chamber of the box body 1, align the puncture guide column 6, press and insert it into the support seat 4 to start the staining process; the waste liquid generated by staining drips onto the second sealing cover and collects into the waste liquid chamber 14 through the liquid inlet 15 on it, and is absorbed by the liquid absorption pillow 16 inside; when the reagents in the staining chamber 11, decolorizing chamber 12 and washing chamber 13 are used up, the box body 1 can be pulled out and disposed of as medical waste.

[0029] It should be noted that in the description of this utility model, terms such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", and "outer" indicating orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

Claims

1. A kit for staining microorganisms, characterized in that: The device includes a housing and a matching base. The housing is divided into a dyeing solution chamber, a decolorizing solution chamber, a washing solution chamber, and a waste solution chamber. The waste solution chamber has an inlet at its top, and the dyeing solution chamber, decolorizing solution chamber, and washing solution chamber each have a downwardly protruding guide port at their bottom, with a liquid seal structure at the end of each guide port. The base contains a storage tank corresponding to the position of each guide port. The top of the storage tank has a support for accommodating the guide ports. The bottom center of the support has an upwardly protruding piercing guide column, which is hollow, with its inlet located on the side wall and its outlet on the bottom surface of the support. The bottom of the storage tank has a liquid guide connector extending beyond the base, which is connected to the waste solution chamber via a liquid delivery pipe.

2. The microbial staining kit according to claim 1, characterized in that: The boxes for the dyeing chamber, decolorizing chamber, and washing chamber are connected by a first sealing cover, and the box for the waste liquid chamber is connected by a second sealing cover. The box corresponding to the first sealing cover is higher than the box corresponding to the second sealing cover.

3. The microbial staining kit according to claim 2, characterized in that: The liquid inlet is located on the second sealing cover, and the second sealing cover has a concave structure that gradually decreases from the periphery towards the liquid inlet.

4. The microbial staining kit according to claim 1, characterized in that: The waste liquid chamber is equipped with a liquid absorption pillow.

5. The microbial staining kit according to claim 1, characterized in that: The box is equipped with handles on its side walls.

6. The microbial staining kit according to claim 1, characterized in that: The liquid seal structure includes a rigid bottle cap screwed onto the outside of the flow guide port. The center of the rigid bottle cap has a through hole corresponding to the position of puncturing the flow guide column. A silicone gasket is provided on the inner side of the rigid bottle cap, and a thermoplastic sealing film is provided on the outer side of the rigid bottle cap.

7. The microbial staining kit according to claim 1, characterized in that: A locking clip is provided on the outer wall of the support, and a matching screw-on locking seat is provided on the inner wall of the liquid storage tank.

8. The kit for microbial staining according to claim 1, characterized in that: The top of the puncture guide column is provided with a puncture tip.

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