Tumor cell invasion and migration behavior analysis device
By designing a device for analyzing tumor cell invasion and migration behavior, the problem that existing technologies cannot simultaneously assess tumor cell invasion and migration has been solved, thus achieving the effect of simultaneously assessing tumor cell invasion and migration.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-10
- Publication Date
- 2026-04-07
AI Technical Summary
Current technologies cannot simultaneously assess the invasion and migration behavior of tumor cells, and Transwell assays cannot perform simultaneous analysis.
A device for analyzing tumor cell invasion and migration behavior was designed, including a film and a cover plate. The film has a tumor cell pool, a chemokine pool, and a migration pool, which are connected by channels. Combined with a microscope and a live cell dynamic imaging instrument, the device simulates the in vivo environment for evaluation.
It enables simultaneous assessment of the invasive and migratory capabilities of tumor cells. It has a simple structure, is easy to operate, and can clearly demonstrate the invasive and migratory capabilities of tumor cells, facilitating accurate assessment.
Smart Images
Figure CN224091899U_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biotechnology, specifically to a device for analyzing tumor cell invasion and migration behavior. Background Technology
[0002] Tumor cells destroy the surrounding matrix by generating and secreting MMPs (matrix metalloproteinases, enzymes that degrade the extracellular matrix), thereby invading surrounding tissues or the vascular system. The invasive potential of tumor cells is significantly affected by factors of the surrounding matrix.
[0003] Currently, the Transwell assay is commonly used to assess tumor cell invasion in vitro. The Transwell assay procedure is as follows: prepare a cell suspension, resuspend and adjust the cell density; seed the cells: add 200 μL of cell suspension to the upper chamber of the Transwell plate; add 500-600 μL of medium containing 10% FBS (fetal bovine serum) to the lower chamber of a 24-well plate; set up a control group containing only serum-free medium; incubate the two groups of cells for 12-48 hours; gently wipe away the uninvaded cells in the upper chamber with a cotton swab or cell scraper, and then fix with methanol; stain and observe under a microscope; however, the Transwell assay cannot simultaneously analyze tumor cell migration and invasion. Utility Model Content
[0004] In order to overcome the shortcomings of the prior art, the purpose of this utility model is to provide a device for analyzing the invasion and migration behavior of tumor cells, so as to quickly and accurately assess the potential of tumor cells to invade the matrix and their migration ability.
[0005] To achieve the above objectives, this utility model provides a device for analyzing tumor cell invasion and migration behavior, including a film and a cover plate bonded to the film. The upper surface of the film is provided with a tumor cell pool, a chemokine pool and a first migration pool. The tumor cell pool and the chemokine pool are connected through a first channel, and the tumor cell pool and the migration pool are connected through a second channel.
[0006] The principle and beneficial effects of the technical solution are as follows: the cover plate is used to shield and seal the film, forming a box shape; the film is the main body of the entire analytical device, and the tumor cell pool on its upper surface is used to add cultured tumor cells; both the chemokine pool and the migration pool can be used to add chemokines, which can be one of 10% fetal bovine serum, C, CC, CXC, or CX3C; the first channel is used to add matrix gel, which can be a simulated liver invasion matrix gel, a simulated bone invasion matrix gel, or a simulated lung invasion matrix gel, etc.; the second channel is not used for observation without adding matrix gel. The migration status of tumor cells; when using this device to assess tumor cell invasion and migration, firstly, matrix gel is added to the first channel at low temperature, then placed in a heating device to simulate human body temperature (36-37°C) for 30-40 minutes. Next, the cultured tumor cell suspension is added to the tumor cell pool and incubated at 36-37°C for 30-40 minutes. Then, chemokines are added to the chemokine pool, and the tumor cell invasion process is observed and recorded using a microscope, live-cell dynamic imaging system, and other equipment. Additionally, 500-600 μL of culture medium containing 10% FBS can be added to the second channel. The migration of tumor cells at 36-37°C is observed and recorded periodically. This device has a simple structure and is easy to operate. The movement of tumor cells in the first and second channels clearly reflects their invasion and migration capabilities, facilitating assessment.
[0007] In a preferred embodiment of this utility model, a rotatable partition ring is provided in the tumor cell pool, and the partition ring is attached to the inner wall of the tumor cell pool; the partition ring is provided with multiple through holes on the side near the first channel and the second channel.
[0008] Beneficial effects: The through holes are used to connect the tumor cell pool with the first channel and the second channel, respectively, so that tumor cells can enter the first channel and the second channel; when the separator ring is rotated at a certain angle, so that the through holes are misaligned with the first channel and the second channel, the tumor cell pool is no longer connected with the first channel and the second channel. At this time, it is convenient to add matrix gel and tumor cell suspension, and avoids spillage into other areas during addition, which would affect the invasion and migration results.
[0009] In a preferred embodiment of this utility model, a rotating strip is fixedly connected above the separating ring, and two limiting blocks are fixed on the base plate. The two limiting blocks are located on both sides of the rotating strip to limit its rotation angle.
[0010] Beneficial effects: The rotating bar facilitates the rotation of the rotating ring, and the two limiting blocks are used to limit the maximum angle of rotation of the rotating bar to both sides.
[0011] In a preferred embodiment of this utility model, the first channel is connected to a third channel, and the third channel is connected to a second migration pool.
[0012] Beneficial effects: The third channel is also used to observe the migration of tumor cells. The difference between the third and second channels is that the tumor cells first invade the matrix gel of the first channel and then enter the third channel to migrate, simulating the situation in the human body.
[0013] In a preferred embodiment of this utility model, the third channel is provided with a partition plate that can slide up and down.
[0014] Beneficial effects: The partition plate is used to separate the first channel and the third channel, preventing the matrix adhesive from overflowing into the third channel when it is added; after the matrix adhesive solidifies, the partition plate can be slid upwards.
[0015] In a preferred embodiment of this utility model, both the substrate and the cover are made of transparent material.
[0016] Beneficial effects: Using transparent materials makes it easier to observe the culture of tumor cell suspensions and the solidification of matrix gel, thus improving practicality. Attached Figure Description
[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.
[0018] Figure 1 This is a schematic diagram of an embodiment of the tumor cell invasion and migration behavior analysis device of this utility model.
[0019] Figure 2 This is a schematic diagram showing the structure of the cover plate hidden in an embodiment of the tumor cell invasion and migration behavior analysis device of this utility model.
[0020] Figure 3 The top view of the embodiment of the tumor cell invasion and migration behavior analysis device of this utility model is shown with the cover plate hidden.
[0021] The reference numerals in the accompanying drawings include: film 1, cover plate 2, tumor cell pool 3, chemokine pool 4, first migration pool 5, first channel 6, second channel 7, septal ring 8, through hole 9, rotating strip 10, limiting block 11, third channel 12, second migration pool 13, and septal plate 14. Detailed Implementation
[0022] The embodiments of this utility model are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the embodiments of this utility model, and should not be construed as limiting the utility model.
[0023] In the description of the embodiments of this utility model, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.
[0024] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the stated features. In the description of the embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.
[0025] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.
[0026] As attached Figures 1-3 As shown, this utility model provides a device for analyzing tumor cell invasion and migration behavior: it includes a film 1 and a cover plate 2 bonded to the film 1. The cover plate 2 is used to cover and seal the film 1, forming a box shape. Both are made of transparent material, such as glass, to facilitate observation of tumor cell suspension culture and matrix gel coagulation. The upper surface of the film 1 is provided with a tumor cell pool 3, a chemokine pool 4, and a first migration pool 5. The tumor cell pool 3 and the chemokine pool 4 are connected by a first channel 6, and the tumor cell pool 3 and the first migration pool 5 are connected by a second channel 7. Cell pool 3 is used to add cultured tumor cells. Chemokine pool 4 and the first migration pool 5 can both be used to add chemokines, which can be one of 10% fetal bovine serum, C, CC, CXC, or CX3C. The first channel 6 is used to add matrix gel, which can be a simulated liver invasion matrix gel, a simulated bone invasion matrix gel, or a simulated lung invasion matrix gel. The second channel 7 is not filled with matrix gel and is used to observe the migration status of tumor cells. The first channel 6 and the second channel 7 are 1-2 cm long, 100 μm-1 mm wide, and 50-200 μm deep.
[0027] As attached Figure 3 As shown, in this embodiment, the first channel 6 is connected to the third channel 12, and the first channel 6 and the third channel 12 are on the same plane. The third channel 12 is provided with a partition plate 14 that can slide up and down. In this embodiment, the third channel 12 is located below the first channel 6, but it can also be located above it. The cover plate 2 is provided with a hole for removing the partition plate 14, which is not shown in the figure. The third channel 12 is connected to the second migration pool 13, which is also used to add chemokines. The partition plate 14 is used to separate the first channel 6 and the third channel 12 to prevent the matrix gel from overflowing into the third channel 12 when it is added. When tumor cells invade through the matrix gel in the first channel 6 and the invasion state is observed, the partition plate 14 can be opened to allow some tumor cells to enter the third channel 12 for migration, simulating the real situation in the human body, that is, the spread of tumor cells first breaks through the basement membrane of the primary lesion by invasion and enters the surrounding tissue or vascular system, and then moves towards blood vessels or lymphatic vessels under the guidance of chemokines.
[0028] As attached Figure 2 and Figure 3 As shown, in this embodiment, a rotatable partition ring 8 is provided in the tumor cell pool 3, and the partition ring 8 is attached to the inner wall of the tumor cell pool 3; the partition ring 8 is provided with multiple through holes 9 on the side near the first channel 6 and the second channel 7; the through holes 9 are respectively used to connect the tumor cell pool 3 with the first channel 6 and the second channel 7, so that tumor cells can enter the first channel 6 and the second channel 7; a rotating strip 10 is fixedly connected above the partition ring 8, and two limiting blocks 11 are fixed on the base plate 1, the two limiting blocks 11 being located on both sides of the rotating strip 10, for limiting its rotation. Angle; at the same time, the cover plate 2 is correspondingly provided with a groove for the rotating bar 10 to rotate; the rotating bar 10 facilitates the rotation of the rotating ring. When the separating ring 8 rotates and abuts against one of the limiting blocks 11, the through hole 9 is connected to the first channel 6 and the second channel 7 respectively. When the separating ring 8 rotates and abuts against the other limiting block 11, the through hole 9 can be offset from the first channel 6 and the second channel 7, so that the tumor cell pool 3 is no longer connected to the first channel 6 and the second channel 7. At this time, it is convenient to add matrix gel and tumor cell suspension, and avoid spillage to other areas during addition, which would affect the invasion and migration results.
[0029] The preferred embodiments of this application have been described in detail above with reference to the accompanying drawings. Typical known structures and common knowledge techniques in the preferred embodiments have not been described in detail here. Those skilled in the art can improve and implement the technical solution of this utility model based on the inspiration given in these embodiments and their own capabilities. Some typical known structures, known methods or common knowledge techniques should not be obstacles for those skilled in the art to implement this application.
[0030] The scope of protection claimed in this application shall be determined by the contents of its claims. The contents of the utility model description, specific embodiments, and drawings are used to interpret the claims.
[0031] Within the scope of the technical concept of this application, several modifications can be made to the specific implementation of this application, and these modified implementations should also be considered within the protection scope of this application.
Claims
1. A device for analyzing tumor cell invasion and migration behavior, characterized in that: The device includes a film and a cover sheet bonded to the film. The upper surface of the film is provided with a tumor cell pool, a chemokine pool and a first migration pool. The tumor cell pool and the chemokine pool are connected by a first channel and the tumor cell pool and the migration pool are connected by a second channel.
2. The tumor cell invasion and migration behavior analysis device according to claim 1, characterized in that: The tumor cell pool is provided with a rotatable partition ring, which is attached to the inner wall of the tumor cell pool; the partition ring is provided with multiple through holes on the side near the first channel and the second channel.
3. The tumor cell invasion and migration behavior analysis device according to claim 2, characterized in that: A rotating bar is fixedly connected above the separating ring, and two limiting blocks are fixed on the base plate. The two limiting blocks are located on both sides of the rotating bar to limit its rotation angle.
4. The tumor cell invasion and migration behavior analysis device according to claim 1, characterized in that: The first channel is connected to a third channel, and the third channel is connected to a second migration pool.
5. The tumor cell invasion and migration behavior analysis device according to claim 4, characterized in that: The third channel is equipped with a partition that can slide up and down.
6. The tumor cell invasion and migration behavior analysis device according to claim 1, characterized in that: Both the substrate and the cover plate are made of transparent material.