Single-sample-adding-hole all-in-one detection device

Abstract

The utility model discloses an all-in-one detection device with a single sample adding hole. The device comprises a shell, a reagent strip arranged in the shell, a solid-liquid structure and a reagent strip mounting structure. A liquid storage chamber which is aligned and communicated with the sample adding hole is formed in the solid-liquid structure; a corresponding reagent placing groove is formed in the liquid storage chamber; and the sample adding area of each reagent strip penetrates through the reagent placing groove and enters the liquid storage chamber. According to the utility model, the liquid storage chamber is formed by arranging the abutted solid-liquid frames on the upper plate and the lower plate, and the sample liquid added into the sample adding hole is restrained by utilizing the liquid storage chamber, so that the sample loss is avoided, and the sample size required by one-time sample adding of a plurality of reagent strips is reduced. According to the utility model, different positions of the guide sheet are pressed by the pressing rib with the rectangular frame structure, so that the guide sheet is fully attached to the reagent strips, and the reagent strips can fully and uniformly absorb sample liquid. Meanwhile, the structure that the guide sheet pressing rib is lower than the bottom edge of the sample adding hole can form a flow channel for sample liquid to diffuse along the guide sheet.

Description

Technical Field

[0001] This utility model belongs to the technical field of liquid sample detection equipment, specifically relating to a single sample well multi-in-one detection device. Background Technology

[0002] In the fields of biological detection and chemical analysis, multi-well plates are commonly used for sample processing and detection. Traditional multi-well plates typically have multiple sample wells and detection windows, requiring multiple sample additions during detection, which is complex and requires a large volume of sample. Therefore, it is necessary to design a scheme with one sample well and multiple detection windows to solve the problem of multiple sample additions and to address the issue of obtaining multiple detection results from a small number of samples. Summary of the Invention

[0003] The purpose of this invention is to provide a single-sample-well multi-function detection device.

[0004] This utility model provides a single-sample-well multi-function detection device, comprising a housing, reagent strips, a solid-liquid structure, and a reagent strip mounting structure disposed within the housing. Multiple reagent strips are respectively mounted on different reagent mounting positions of the reagent strip mounting structure. The housing has a sample dispensing hole and an observation area; the display area of ​​each reagent strip is located within the observation area; a liquid storage chamber is formed in the solid-liquid structure, aligned with and connected to the sample dispensing hole; the liquid storage chamber has a corresponding reagent placement slot; the sample dispensing area of ​​each reagent strip passes through the reagent placement slot and enters the liquid storage chamber.

[0005] Preferably, the housing includes an upper plate and a lower plate that are fixed to each other. The sample application port and the observation area are both located on the upper plate.

[0006] Preferably, the solid-liquid structure includes a first solid-liquid frame located on the inner side of the upper plate, and a second solid-liquid frame integrally formed on the inner side of the lower plate. The first and second solid-liquid frames are positioned correspondingly and abut against each other to form a liquid storage chamber.

[0007] Preferably, the system also includes a guide plate disposed in the liquid storage chamber; the solid-liquid structure further includes a guide plate rib located inside the first solid-liquid frame; the guide plate is aligned with the sample dispensing hole and, constrained by the guide plate rib, holds all reagent strips in place.

[0008] Preferably, the guide strip ribs have a frame-shaped structure. The inner space of the guide strip ribs intersects with the sample application areas of all reagent strips.

[0009] Preferably, the bottom edge of the guide plate rib is lower than the bottom edge of the sample dispensing hole, so that a liquid flow channel is formed between the bottom edge of the sample dispensing hole and the guide plate.

[0010] Preferably, the reagent strip mounting structure includes a reagent clamping structure and a support column on the upper plate, and a reagent support structure, side ribs, and end ribs on the lower plate. The end of the support column abuts against the lower plate; the side ribs and end ribs provide length and width limits for the reagent strip. The reagent strip is clamped between the reagent clamping structure and the reagent support structure.

[0011] Preferably, the bottom surface of the liquid storage chamber is provided with a hydrophilic coating; the sample application area of ​​all reagent strips is in contact with the bottom surface of the liquid storage chamber.

[0012] Preferably, the sample application area of ​​all reagent strips is in contact with the bottom surface of the reservoir chamber. The bottom surface of the reservoir chamber is provided with one or more capillary grooves; the capillary grooves extend along the arrangement direction of the reagent strips.

[0013] Preferably, the observation area has n independent observation windows arranged at intervals. n is the number of reagent strips, ranging from 2 to 8; each observation window corresponds to one reagent strip.

[0014] The present invention has the following beneficial effects.

[0015] 1. This invention utilizes the diffusion of sample liquid in the same sampling well to achieve the addition of multiple test strips, thereby enabling the detection of multiple test strips with only one addition, resulting in high addition efficiency and saving sample. Furthermore, this invention forms a liquid storage chamber by setting abutting solid-liquid frames on the upper and lower plates, which constrains the sample liquid added to the sampling well, preventing sample loss and reducing the amount of sample required for adding multiple test strips at one time.

[0016] 2. This invention uses a rectangular frame structure with ribs to press down on different positions of the guide plate, ensuring that the guide plate fully adheres to the reagent strip and that each reagent strip can fully and evenly absorb the sample solution. Simultaneously, the structure of the guide plate ribs being lower than the bottom edge of the sample dispensing hole creates a flow channel for the sample solution to diffuse along the guide plate, allowing the sample solution to flow more quickly and fully to all reagent strips.

[0017] 3. This utility model sets a hydrophilic coating or capillary groove in the liquid storage chamber formed by the solid-liquid frame, which realizes the rapid diffusion of sample liquid without the need for guide sheet, and ensures uniform sample addition of each reagent strip while simplifying the structure.

[0018] 4. This invention features multiple supporting ribs on the lower plate, which control the gap between each position of the reagent strip and the upper plate, ensuring smoother reagent strip chromatography, preventing interference between reagent strips, and improving testing accuracy. Simultaneously, the multiple limiting ribs on the lower plate reduce reagent strip positional deviation, ensuring optimal sample absorption and observation. Attached Figure Description

[0019] Figure 1 This is an exploded view of Embodiment 1 of the present invention.

[0020] Figure 2 This is a perspective view of Embodiment 1 of the present utility model.

[0021] Figure 3 This is a schematic diagram of the inner structure of the upper plate in Embodiment 1 of this utility model.

[0022] Figure 4 This is a schematic diagram of the inner structure of the lower plate in Embodiment 1 of this utility model.

[0023] Figure 5 This is a structural comparison diagram of Embodiment 1 of this utility model with different numbers of reagent strips.

[0024] Figure 6 This is a schematic diagram of the inner structure of the lower plate in Embodiment 2 of this utility model.

[0025] Figure 7 This is a schematic diagram of the inner structure of the lower plate in Embodiment 3 of this utility model.

[0026] Figure 8 This is a structural comparison diagram of Embodiment 4 of this utility model with different numbers of reagent strips. Detailed Implementation

[0027] The present invention will be further described below with reference to the accompanying drawings.

[0028] Example 1

[0029] like Figure 1 and Figure 2 As shown, a single-well multi-function detection device has a rectangular plate-like structure, including a housing, a reagent strip 300, a guide plate 400, a solid-liquid structure, and a reagent strip mounting structure disposed within the housing. The housing includes an upper plate 100 and a lower plate 200 stacked and fixed together. An application port 102 and an observation area 101 are provided on the outer surface of the upper plate 100. The application port 102 and the observation area 101 are arranged along the length of the housing. The observation area 101 has three elongated slots arranged at equal intervals along the width of the housing. A separator strip integrally formed on the upper plate 100 is provided between each adjacent elongated slot; each elongated slot serves as an observation window 1011. Each observation window 1011 corresponds to one reagent strip 300. The same application port 102 is used simultaneously to provide liquid samples to all reagent strips 300.

[0030] The reagent strip mounting structure is housed within the casing, forming three reagent mounting positions arranged at intervals along the width of the casing. Three reagent strips 300 are respectively mounted on the three reagent mounting positions. The display areas of the three reagent strips 300 are aligned with the three observation windows. The reagent strips are parallel to each other and arranged in a straight line.

[0031] like Figure 3 and Figure 4 As shown, the solid-liquid structure is aligned with the sample feeding hole 102 and includes a first solid-liquid frame 107 integrally formed on the inner side of the upper plate 100, a guide plate pressure rib 108, a liquid guiding channel 109, and a second solid-liquid frame 202 integrally formed on the inner side of the lower plate 200.

[0032] Both the first solid-liquid frame 107 and the second solid-liquid frame 202 are hollow rectangular structures with matching shapes. The first solid-liquid frame 107 and the second solid-liquid frame 202 abut together, forming a liquid storage chamber. The guide plate 400 is disposed inside the second solid-liquid frame 202. In this embodiment, the guide plate is made of hydrophilic fiber or hydrophilic plastic sheet; the liquid storage chamber is used to constrain the flow range of the sample liquid, reduce liquid overflow, prevent sample loss, and ensure the accuracy of the detection results.

[0033] The inner side of the second solid-liquid frame 202 is provided with a first reagent support rib 2022 integrally formed on the inner side of the lower plate 200. The number of the first reagent support ribs 2022 is one or multiple ribs arranged at intervals; the height of each support rib can be the same or different; the extension direction of the support rib can be parallel to the length direction of the reagent strip or perpendicular to the length direction of the reagent strip.

[0034] The second solid-liquid frame 202 has three reagent placement slots 2021 on its side wall near the reagent strip mounting structure; each reagent placement slot 2021 corresponds to a reagent mounting position. The three reagent strips 300 pass through the three reagent placement slots 2021 on the second solid-liquid frame 202 respectively; the sample application area of ​​the reagent strip is located inside the second solid-liquid frame 202 and is supported on the first reagent support rib 2022.

[0035] The guide plate 400 is supported on the sample application area of ​​the reagent strip. The guide plate 400 is made of a hydrophilic material, which facilitates rapid diffusion of the sample solution. The guide plate rib 108 is rectangular and located inside the first solid-liquid frame 107. The guide plate rib 108 flattens the guide plate 400, ensuring uniform adhesion to the reagent strip. The inner space of the guide plate rib 108 intersects with the sample application areas of all three reagent strips to prevent the guide plate rib 108 from obstructing sample solution diffusion and affecting the sample solution's arrival at the reagent strip.

[0036] The sample dispensing hole 102 has a funnel-shaped conical hole structure. The bottom edge of the guide plate pressure rib 108 is lower than the bottom edge of the sample dispensing hole 102, forming a height difference; this height difference causes a liquid guiding channel 109 to be formed between the bottom edge of the sample dispensing hole 102 and the guide plate 400; the liquid guiding channel 109 can prevent the bottom edge of the sample dispensing hole 102 from directly squeezing the guide plate 400, so that the sample liquid added to the sample dispensing hole 102 can quickly diffuse to both sides along the liquid guiding channel 109.

[0037] like Figure 3 and Figure 4 As shown, the reagent strip mounting structure includes a reagent clamping column 104, a reagent clamping rib 105, and a support column 106 integrally formed on the inner side of the upper plate 100, as well as a second reagent support rib 203, a third reagent support rib 204, a fourth reagent support rib 205, a first side baffle rib 206, a second side baffle rib 207, and an end baffle rib 209 integrally formed on the inner side of the lower plate 200.

[0038] Each reagent mounting position is equipped with one or more reagent clamping posts 104 and one or more reagent clamping ribs 105. The reagent clamping ribs 105 are located between the sample dispensing hole 102 and the observation area 101; the reagent clamping posts 104 are located on the side of the observation area 101 away from the sample dispensing hole 102. Both the reagent clamping ribs 105 and the reagent clamping posts 104 are used to clamp the corresponding reagent strip to prevent the reagent strip from shifting.

[0039] The support column 106 is positioned offset from the reagent mounting position; in this embodiment, a support column 106 is provided between any two adjacent reagent mounting positions. The end of the support column 106 contacts the inner surface of the lower plate 200. The support column 106 is used to support the upper plate, maintaining a constant distance between the upper and lower plates, and the number of support columns can be one or more.

[0040] Each reagent mounting position is provided with a set of sequentially arranged second reagent support ribs 203, third reagent support ribs 204, fourth reagent support ribs 205, and end retaining ribs 209. The second reagent support ribs 203, third reagent support ribs 204, fourth reagent support ribs 205, and end retaining ribs 209 are arranged sequentially in a direction away from the second solid-liquid frame 202. The reagent strip is supported on each reagent support rib, and its end is limited by the end retaining ribs 209.

[0041] Between any two reagent mounting positions, on opposite sides of the two reagent mounting positions located at the edge, there are first side baffles 206 and second side baffles 207. The position of the first side baffle 206 is aligned with the fourth reagent support rib 205. The second side baffle 207 is located on the side of the first side baffle 206 away from the second solid-liquid frame 202.

[0042] Multiple limiting protrusions 2061 are provided on the opposite sides of any two adjacent first side baffles 206; the distance between the limiting protrusions 2061 on the two first side baffles 206 is greater than or equal to the width of the reagent strip.

[0043] Multiple sets of locking points 2071 are symmetrically arranged on the opposite sides of any two adjacent second side baffles 207; the distance between the locking points 2071 on the two second side baffles 207 is less than the width of the reagent strip. The locking point has a sharp corner structure on the side closer to the reagent strip.

[0044] In some embodiments, a fifth reagent support rib 2072 is provided between two adjacent second side baffles 207, and the number of such ribs is one or more.

[0045] In some embodiments, side baffles are also provided at the locations of the second reagent support rib 203 and the third reagent support rib 204; the side baffles are used to prevent the reagent strip from shifting position.

[0046] In some embodiments, each baffle 209 is further provided with a sixth reagent support rib 208 on the side near the second solid-liquid frame 202.

[0047] Multiple mounting posts 103 are provided on the inner edge of the upper plate 100; multiple mounting holes 201 are provided on the inner edge of the lower plate 200; the number of mounting posts 103 and the number of mounting holes 201 are the same, and their positions correspond one-to-one. The corresponding mounting posts 103 and mounting holes 201 are inserted together to form an interference fit, thereby fixing the upper plate 100 and the lower plate 200. The number of mounting posts 103 is two or more.

[0048] In this embodiment, the assembly column is hexagonal prism-shaped; in other embodiments, the assembly column can also be other prism structures such as triangular prism, square prism, pentagonal prism, heptagonal prism, octagonal prism, or cylindrical. The prism structure provides better assembly with the assembly hole, and the assembly hole is less prone to cracking.

[0049] In this embodiment, the assembly holes are circular. In other embodiments, the assembly holes may also be oblong or other hole types that can form an interference fit with the assembly column 103. In some further preferred embodiments, some assembly holes are circular and others are oblong, which can avoid assembly deformation caused by the tolerance of the assembly column.

[0050] In some embodiments, multiple assembly columns are arranged in an asymmetrical manner to prevent incorrect assembly, ensuring that there is only one assembly method between the upper and lower plates, thus avoiding assembly errors.

[0051] In some other embodiments, the sample application area of ​​the upper plate 100 has only one integral through slot; the through slot covers the display area of ​​all reagent strips.

[0052] In some other embodiments, the number of reagent strips is not three, but rather 2, 4, 5, 6, 7, 8, or other desired numbers; when the number of reagent strips changes, the width of the solid-liquid structure, the number of reagent mounting positions in the reagent strip mounting structure, and the number of observation windows change accordingly. The front structure of the detection device with different numbers of reagent strips is shown below. Figure 5 As shown.

[0053] In some embodiments, the observation area 101 is provided with a concave inclined structure around its perimeter; the inclined structure allows the observation window 1011 to be closer to the display area of ​​the reagent strip and makes the surrounding light of the observation window 1011 more sufficient, which is more conducive to user reading.

[0054] In some embodiments, the label pad in the test strip is made of glass fiber instead of the common polyester fiber membrane pad, resulting in more uniform release of the sample solution. Therefore, compared with conventional test strips using polyester fiber membrane pads, the sensitivity, specificity, and uniformity of the test strip in this embodiment are improved.

[0055] In some embodiments, the test strip is used to detect the novel coronavirus (SARS-CoV-2). The antibody used in the test strip is the highly conserved nucleocapsid protein of mouse anti-SARS-CoV-2, with detection epitopes of 44-175aa and 74-105aa, which can effectively avoid high-frequency sites of viral mutations and greatly improve the clinical detection rate of the reagent and its inclusiveness to different mutant virus strains.

[0056] The working principle of this embodiment is as follows:

[0057] The sample solution to be tested is added dropwise into the sample well 102. The sample solution quickly diffuses to both sides through the guide plate 400, wetting the sample application area of ​​all reagent strips. Each reagent strip is run simultaneously; after waiting for the preset time, the test results of each reagent strip are read through the observation window. The reagent strips can be of the same type or different types.

[0058] Example 2

[0059] A single-sample-addition-well multi-function detection device, the difference between this embodiment and embodiment 1 is that the guide plate 400, guide plate pressure rib 108 and reagent support rib 2022 are not provided.

[0060] like Figure 6 As shown, in this embodiment, the portion of the lower plate 200 inside the second solid-liquid frame 202 is flat and has a hydrophilic coating 2023. The hydrophilic coating can promote the diffusion of the sample liquid, so that the sample liquid can quickly contact the sample application area of ​​all reagent strips, playing a role similar to the guide sheet 400 in Embodiment 1.

[0061] In this embodiment, the portion of the lower plate 200 inside the second solid-liquid frame 202 is raised as a whole, so that the sample application area of ​​the reagent strip is in direct contact with the hydrophilic coating.

[0062] The other structures in this embodiment are the same as in Embodiment 1.

[0063] Example 3

[0064] A single-sample-addition-well multi-function detection device, the difference between this embodiment and embodiment 1 is that the guide plate 400, guide plate pressure rib 108 and reagent support rib 2022 are not provided.

[0065] like Figure 7 As shown, in this embodiment, the lower plate 200 has multiple capillary grooves 2024 on the inner side of the second solid-liquid frame 202, with a groove width of 0.1mm to 2mm. The capillary grooves 2024 extend along the arrangement direction of the reagent strips. The capillary grooves 2024 can promote the diffusion of the sample liquid, so that the sample liquid can quickly contact the sample application area of ​​all reagent strips, playing a role similar to the guide plate 400 in Embodiment 1.

[0066] The other structures in this embodiment are the same as in Embodiment 1.

[0067] Example 4

[0068] A single-sample-well multi-function detection device is provided. The difference between this embodiment and Embodiment 1 is that the shape of the shell is different and the arrangement of the reagent strips is different.

[0069] In this embodiment, the shell has an arc-shaped structure that is wider at one end and narrower at the other, and its shape is approximately oval. The reagent strips inside the shell are not parallel, but arranged in a fan shape. Along the direction from the sample application port 102 to the observation area 101, adjacent reagent strips are spaced far apart. The included angle between two adjacent reagent strips is 1° to 30°; the number of reagent strips can be freely set according to actual detection needs, for example, 2, 3, 4, 5, 6, 7, or 8. The front structure of the detection device with different numbers of reagent strips is shown below. Figure 8 As shown.

[0070] In this embodiment, the sample application areas of each reagent strip are close to each other, which helps to reduce the size of the solid-liquid structure and the guide plate 400, and further accelerates the speed at which the sample solution wets the sample application areas of each reagent strip; the display areas of each reagent strip are far apart from each other, which helps the user to read the test results of each reagent strip separately.

Claims

1. A single-well multi-function detection device, comprising a housing, a reagent strip (300) disposed within the housing, and a reagent strip mounting structure; multiple reagent strips (300) are respectively mounted on different reagent mounting positions of the reagent strip mounting structure; the housing is provided with a sample dispensing hole (102) and an observation area (101); characterized in that: The single-sample-hole multi-function detection device also includes a solid-liquid structure located inside the housing; a liquid storage chamber is formed in the solid-liquid structure that is aligned with and connected to the sample-dispensing hole (102); the liquid storage chamber is provided with a corresponding reagent placement slot (2021); the sample dispensing area of ​​each reagent strip (300) passes through the reagent placement slot (2021) and enters the liquid storage chamber.

2. The single-sample-well multi-function detection device according to claim 1, characterized in that: The housing includes an upper plate (100) and a lower plate (200) that are fixed to each other; the sample application hole (102) and the observation area (101) are both located on the upper plate (100).

3. The single-sample-well multi-function detection device according to claim 2, characterized in that: The solid-liquid structure includes a first solid-liquid frame (107) located on the inner side of the upper plate (100) and a second solid-liquid frame (202) integrally formed on the inner side of the lower plate (200); the first solid-liquid frame (107) and the second solid-liquid frame (202) are positioned correspondingly and abut against each other to form a liquid storage chamber.

4. The single-sample-well multi-function detection device according to claim 3, characterized in that: It also includes a guide plate (400) disposed in the liquid storage chamber; the solid-liquid structure also includes a guide plate rib (108) located inside the first solid-liquid frame (107); the guide plate (400) is aligned with the sample dispensing hole (102) and abuts against all reagent strips (300) under the constraint of the guide plate rib (108).

5. The single-sample-well multi-function detection device according to claim 4, characterized in that: The guide strip rib (108) has a frame-shaped structure; the inner space of the guide strip rib (108) intersects with the sample application area of ​​all reagent strips.

6. The single-sample-well multi-function detection device according to claim 4, characterized in that: The bottom edge of the guide plate rib (108) is lower than the bottom edge of the sample feeding hole (102), so that a liquid flow channel (109) is formed between the bottom edge of the sample feeding hole (102) and the guide plate (400).

7. The single-sample-well multi-function detection device according to claim 2, characterized in that: The reagent strip mounting structure includes a reagent clamping structure and a support column (106) located on the upper plate (100), and a reagent support structure, side baffles and end baffles (209) located on the lower plate (200); the end of the support column (106) abuts against the lower plate (200); the side baffles and end baffles (209) provide length and width limits for the reagent strip; the reagent strip is clamped between the reagent clamping structure and the reagent support structure.

8. The single-sample-well multi-function detection device according to claim 1, characterized in that: The bottom surface of the liquid storage chamber is coated with a hydrophilic coating; the sample application area of ​​all reagent strips is in contact with the bottom surface of the liquid storage chamber.

9. The single-sample-well multi-function detection device according to claim 1, characterized in that: The sample application area of ​​all reagent strips is in contact with the bottom surface of the reservoir chamber; the bottom surface of the reservoir chamber is provided with one or more capillary grooves (2024); the capillary grooves (2024) extend along the arrangement direction of the reagent strips.

10. A single-sample-well multi-function detection device according to claim 1, characterized in that: The observation area (101) is provided with n independent observation windows (1011) arranged at intervals in sequence; n is the number of reagent strips (300), and the value is 2 to 8; the observation window (1011) corresponds one-to-one with the reagent strip (300).

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