Abdominal aortic aneurysm susceptibility gene screening kit

Abstract

The utility model discloses an abdominal aortic aneurysm susceptibility gene screening kit which comprises a tool box and an L-shaped working box, the L-shaped working box comprises a vertical part and a horizontal part, and the tool box is located on the horizontal part of the L-shaped working box; a plurality of clamping grooves are formed in the bottom in the horizontal part of the L-shaped working box, reagent strips are arranged in the clamping grooves, sample application areas, a strip I and a strip II are arranged on the reagent strips, sample adding holes are formed in the vertical part of the L-shaped working box and correspond to the reagent strips respectively, rotating covers are arranged on the sample adding holes, and bottom outlets of the sample adding holes correspond to the sample application areas of the reagent strips up and down. And a drawable baffle is arranged at the bottom of the sample adding hole. The kit is provided with a plurality of sample adding holes and a plurality of reagent strips, so that multi-gene joint detection is realized at the same time, and the detection result is more accurate.

Description

Technical Field

[0001] This invention relates to a reagent kit, and more particularly to a reagent kit for screening susceptibility genes for abdominal aortic aneurysm. Background Technology

[0002] Abdominal aortic aneurysm (AAA) is a highly insidious and deadly vascular disease, making early screening crucial for its prevention and treatment. Current technologies, such as gene screening kits, assess risk by detecting susceptibility genes associated with AAA (e.g., IL-6, MMP-9, TGF-β), but these methods have the following drawbacks:

[0003] Insufficient sensitivity and specificity: Existing kits are mostly based on the detection of single and common gene loci, which have a certain reactivity to a variety of cardiovascular diseases, making it difficult to achieve precise targeting of AAA, resulting in a high false negative / positive rate.

[0004] Operational complexity and cost: Traditional reagent kits rely on PCR amplification and sequencing technologies, requiring specialized equipment and personnel, resulting in long testing cycles and high costs, which limits their widespread application in primary healthcare institutions. This reagent kit is simple to operate, requiring only a small amount of blood sample for immediate reaction and imaging, greatly reducing technical barriers.

[0005] Stability and storage conditions: The reaction part of the kit adopts the CRISPR-CAS12 system, which can be stored and reacted at room temperature, reducing the difficulty of commercialization.

[0006] The core reason for the aforementioned problems lies in the fact that existing technologies have failed to effectively integrate multi-gene joint detection, rapid nucleic acid extraction, and stable reagent formulations. This application addresses these technological bottlenecks by proposing a novel reagent kit design. Through optimizing gene locus combinations, introducing room-temperature stable enzyme preparations, and simplifying the operation process, it aims to achieve high-sensitivity, low-cost, and portable screening. Utility Model Content

[0007] The purpose of this utility model is to solve the problems of insufficient smooth grain circulation and the large number of parts used in the existing technology, and to propose a dust suppression hopper for grain warehouse discharge.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A susceptibility gene screening kit for abdominal aortic aneurysm includes a toolbox and an L-shaped work box. The L-shaped work box includes a vertical part and a horizontal part, with the toolbox located in the horizontal part. The bottom of the horizontal part of the L-shaped work box has several slots, each containing a reagent strip. Each reagent strip has a spotting area and two bands (I and II) for color development. The vertical part of the L-shaped work box has a sample application hole corresponding to each reagent strip, with a rotating cap on each hole. The bottom outlet of the sample application hole corresponds vertically to the spotting area of ​​the reagent strip, and the bottom of the sample application hole has a pull-out baffle.

[0010] Furthermore, a transparent cover is provided on the top of the horizontal section of the L-shaped work box.

[0011] Furthermore, the toolbox includes a toolbox lid and a toolbox body, and the toolbox body contains an instruction manual, operating gloves, blood collection needles, iodine swabs, and disinfectant wipes.

[0012] Furthermore, a filter membrane is provided at the bottom of the sample loading hole, and the reaction system liquid is placed inside the sample loading hole.

[0013] This application proposes an improvement to the technical shortcomings of the abdominal aortic aneurysm susceptibility gene screening kit, which has the following significant advantages compared with the existing technology:

[0014] 1. Enhanced multi-gene collaborative detection capabilities

[0015] By optimizing the screening of multi-gene combinations highly associated with abdominal aortic aneurysm, simultaneous detection of multiple gene loci is achieved, and the single disease of abdominal aortic aneurysm is precisely targeted, significantly improving the sensitivity and accuracy of risk assessment (the false negative rate is reduced by about 15%-20%), and more accurately reflecting the gene synergistic pathogenic mechanism.

[0016] 2. Simplified operation process and reduced costs

[0017] Adopting an integrated reagent kit design, it integrates rapid nucleic acid extraction, amplification and detection modules, eliminating the need for PCR instruments or sequencing equipment, reducing operation steps by more than 50%, shortening the detection time to within 1 hour, and reducing reagent costs by about 30%, making it suitable for primary healthcare institutions and home self-testing scenarios.

[0018] 3. Enhanced reagent stability

[0019] The reaction system and enzyme reagents can be stored at room temperature (4-30℃) for at least 12 months, breaking through the limitations of traditional cold chain transportation, reducing storage and logistics costs, and are especially suitable for promotion in resource-scarce areas.

[0020] 4. Primer design optimization to improve specificity

[0021] By employing highly specific primer combinations and anti-cross-reaction design, non-specific amplification is reduced, and the detection specificity is increased to over 98% (compared to an average of 85%-90% for traditional techniques), effectively reducing the risk of false positives and minimizing reagent waste caused by primer redundancy.

[0022] 5. Portability and rapid screening capabilities

[0023] The kit features a miniaturized, pre-packaged design, supporting a rapid "sample in, result out" testing mode (e.g., completed within 30 minutes), meeting the needs of point-of-care testing (POCT), and is suitable for scenarios such as emergency screening and community health checkups.

[0024] 6. Enhanced environmental adaptability

[0025] By improving the buffer formulation of the reaction system, the kit can operate stably over a wide temperature range (15-40℃) and under different humidity conditions, reducing dependence on standardized laboratory environments and improving reliability in practical applications.

[0026] 7. The reagent kit is completely sealed.

[0027] Compared with traditional nucleic acid test strips, this kit fixes the reaction area and the color development area in the same system. Users only need to draw blood samples and add them to the sample port. Subsequent reaction steps are all completed in a closed space, reducing the possibility of infection and greatly simplifying the operation steps, thus expanding the target audience of the kit. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall design of this utility model;

[0029] Figure 2 This is a structural diagram of the toolbox;

[0030] Figure 3 This is a structural diagram of an L-shaped work box;

[0031] Figure 4 This is a schematic diagram of the disassembled L-shaped work box;

[0032] Figure 5 This is a schematic diagram illustrating the principle of color development for reagent strip I.

[0033] Figure 6 This is a schematic diagram illustrating the principle of color development for reagent strip II.

[0034] In the diagram: 1. Card slot; 2. Tool box; 201. Tool box cover; 202. Tool box body; 3. Rotating cover; 4. L-shaped work box; 5. Sample dispensing hole; 6. Reagent strip; 601. Sample spotting area; 7. Pull-out baffle; 8. Transparent cover. Detailed Implementation

[0035] The technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments.

[0036] A screening kit for susceptibility genes to abdominal aortic aneurysm, such as Figures 1 to 4 As shown, the kit includes a tool box 2 and an L-shaped work box 4. The L-shaped work box 4 includes a vertical part and a horizontal part. The tool box 2 is located in the horizontal part of the L-shaped work box 4, making the kit as a whole rectangular prism. The bottom of the horizontal part of the L-shaped work box 4 is provided with several slots 1. The slots 1 are provided with reagent strips 6. The reagent strips 6 are provided with a spotting area 601 and I strips and II strips for color development. The vertical part of the L-shaped work box 4 is provided with a sample dispensing hole 5 corresponding to each reagent strip 6. The sample dispensing hole 5 is provided with a rotating cover 3. The bottom outlet of the sample dispensing hole 5 corresponds vertically to the spotting area of ​​the reagent strip 6. The bottom of the sample dispensing hole 5 is provided with a pull-out baffle 7.

[0037] The top of the horizontal section of the L-shaped work box 4 is provided with a transparent cover 8.

[0038] Tool box 2 includes tool box cover 201 and tool box body 202. Tool box body 202 contains instruction manual, operating gloves, blood collection needle, iodine swabs and disinfectant wipes.

[0039] A filter membrane (not shown in the figure) is provided at the bottom of the sample loading port 5, and the reaction system liquid is placed inside the sample loading port 5.

[0040] The process of using this utility model:

[0041] The patient opened the outer packaging of the abdominal aortic aneurysm susceptibility gene screening kit and obtained a toolbox 2 and a work box 4. The patient opened toolbox 2, learned the operating procedures, and used the iodine swabs to disinfect the area before drawing blood with the lancet.

[0042] Unscrew the rotating cap 3 above the sample well 5, drop blood into the sample well 5, and tighten the rotating cap 3 (to reduce the possibility of contamination). Gently shake to fully mix the blood sample with the buffer solution, and let it stand. The reaction will be complete in about 30 minutes. Pull out the pull-out baffle 7 to allow the sample to slowly drip onto the spotting area 601 of the reagent strip 6 below.

[0043] Taking colloidal gold reagent strips as an example, such as Figure 5 and Figure 6 As shown, the liquid chromatographically passes through each region along the colloidal gold reagent strip, and the results are visualized. From the spotting area 601 outwards, the zones are: the embedding area (gold microspheres, FITC antibody), band I (streptomycin - bound to biotin), and band II (goat anti-mouse antibody - bound to FITC). The sample chromatographically passes through these regions sequentially along the test strip.

[0044] When the enzyme in the buffer recognizes the target nucleic acid, it cleaves the signal molecule (carrying BIOTIN / FITC at both ends). The signal molecule binds to the gold microspheres through the FITC end of the embedding region, but not to the biotin end. At band I, the biotin end binds to streptomycin, resulting in no color development. At band II, the FITC end binds to goat anti-mouse antibody, causing the gold microspheres to precipitate and develop color. A positive result (+) is indicated by no color development at band I and color development at band II.

[0045] When the enzyme in the buffer does not recognize the target nucleic acid, it does not cleave the signal molecule (which carries BIOTIN / FITC at both ends). The signal molecule binds to the gold microspheres through the FITC end of the embedding region. At band I, the biotin end of the signal molecule binds to streptomycin, and the gold microspheres precipitate and develop color. At band II, no binding occurs and no color development occurs. This results in a negative result (-): band II shows no color development, and band I shows color development.

[0046] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A screening kit for susceptibility genes to abdominal aortic aneurysm, characterized in that, The device includes a toolbox and an L-shaped work box. The L-shaped work box has a vertical section and a horizontal section, with the toolbox located in the horizontal section. The bottom of the horizontal section of the L-shaped work box has several slots, each containing a reagent strip. Each reagent strip has a spotting area and two bands (I and II) for color development. The vertical section of the L-shaped work box has a sample dispensing hole corresponding to each reagent strip. Each sample dispensing hole has a rotating cap, and the bottom outlet of the dispensing hole corresponds vertically to the spotting area of ​​the reagent strip. The bottom of the dispensing hole has a pull-out baffle.

2. The abdominal aortic aneurysm susceptibility gene screening kit according to claim 1, wherein The top of the horizontal section of the L-shaped work box is equipped with a transparent cover.

3. The abdominal aortic aneurysm susceptibility gene screening kit according to claim 1, wherein The toolbox includes a lid and a body. Inside the toolbox are an instruction manual, operating gloves, a blood collection needle, iodine swabs, and disinfectant wipes.

4. The abdominal aortic aneurysm susceptibility gene screening kit according to claim 1, wherein A filter membrane is installed at the bottom of the sample loading hole, and the reaction system liquid is placed inside the sample loading hole.

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