Environment DNA active collection device based on unmanned aerial vehicle carrying

Abstract

The utility model relates to the technical field of environmental DNA collection, and discloses an environmental DNA active collection device carried on the basis of an unmanned aerial vehicle, which comprises an active enrichment power unit and a sample enrichment unit which are arranged at the bottom of an unmanned aerial vehicle body, and the active enrichment power unit is connected to one end of the sample enrichment unit. According to the environment DNA active collection device carried based on the unmanned aerial vehicle, the active enrichment power unit and the sample enrichment unit are arranged, the sample enrichment unit loads a DNA enrichment membrane to the unmanned aerial vehicle body, an electric pump of the active enrichment power unit rapidly adsorbs surrounding air, the air passes through the DNA enrichment membrane on the sample enrichment unit, and the DNA enrichment membrane is separated from the sample enrichment unit; the unmanned aerial vehicle body drives the sample enrichment unit to be close to the air above various ecological systems to collect environmental DNA, more biological information is rapidly collected through the active enrichment power unit, the problem that the DNA content in the air is extremely low and is difficult to enrich is solved, and the biological DNA collection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to environmental DNA collection technical field, concretely is an environmental DNA active collection device based on unmanned aerial vehicle. BACKGROUND

[0002] eDNA, environmental DNA, refers to the mixture of all discovered genomic DNA of different organisms in environmental samples, which can include soil, sediment, excrement, air, water, etc., the main role of eDNA is to obtain taxonomic information and gene function information of species by analyzing these DNA, so as to understand the species existing in the corresponding environment. Through the eDNA technology of collecting biological DNA fragments in the environment and sequencing, it is increasingly applied to ecological environment monitoring and biodiversity protection. Compared with the traditional species investigation method, eDNA technology can quickly obtain rich genetic information and identify a variety of species, including elusive species that are difficult to observe, thereby improving the efficiency and accuracy of species monitoring.

[0003] At present, the DNA collection method in the environment is mainly divided into two kinds, one is liquid impact type collection method, directly using sterile water or PBS buffer as sample adsorption liquid, after collection, the adsorption liquid containing air biological DNA is centrifuged or filtered and concentrated to extract DNA, the other is sterile filter membrane collection method, the sterile filter membrane is suspended in the air or placed in the fixed sampler, after collection, the filter membrane is washed with sterile water or PBS buffer, the deposited particles on the filter membrane are dissolved therein, and the DNA is extracted by subsequent precipitation.

[0004] Compared with the eDNA collection of soil, water or sediment, the eDNA collection in the air has great challenges in ecological monitoring, because the DNA in the air is extremely small, the source and composition of the air sample are difficult to control accurately, and it is easily affected by external environment, such as wind speed, humidity, temperature and other factors, resulting in the difficulty of rapid enrichment of eDNA in the air. SUMMARY

[0005] In view of the defects of the prior art, the utility model provides an environmental DNA active collection device based on unmanned aerial vehicle, which solves the problems mentioned in the above background.

[0006] The utility model provides the following technical scheme: an environmental DNA active collection device based on unmanned aerial vehicle, comprising: an active enrichment power unit and a sample enrichment unit arranged at the bottom of the unmanned aerial vehicle body, the active enrichment power unit is connected to one end of the sample enrichment unit;

[0007] The active enrichment power unit comprises an electric pump and a ventilation pipeline, the electric pump is installed at the bottom of the UAV body, and the ventilation pipeline is installed at the air inlet end of the electric pump.

[0008] The sample enrichment unit comprises a mounting box arranged at the bottom of the UAV body, a surface of the mounting box is connected with one end of the ventilation pipeline, a layout plate is arranged on the mounting box, and at least one DNA enrichment membrane is arranged on the layout plate.

[0009] Preferably, a protective cover is arranged on the surface of the mounting box, and a moving assembly is arranged on the surface of the mounting box away from the layout plate.

[0010] Preferably, the moving assembly comprises a linear motor or a battery, a connecting plate is arranged on the moving block of the linear motor, and the surface of the connecting plate is connected with the surface of the protective cover.

[0011] Preferably, a guide rail is arranged on the surface of the mounting box, a guide block is arranged on the surface of the guide rail, a connecting frame is arranged on the surface of the guide block, and the surface of the connecting frame is connected with the surface of the protective cover.

[0012] Preferably, clamping plates are arranged on the top and bottom of the layout plate, recesses are arranged on the surface of the clamping plates, protrusions are arranged on the surface of the mounting box corresponding to the recesses, and the protrusions are clamped on the inner walls of the recesses.

[0013] Preferably, a supporting frame is arranged on the surface of the mounting box and arranged at the bottom of the UAV.

[0014] Preferably, the DNA enrichment membrane is made of nitrocellulose membrane, polycarbonate membrane or glass fiber membrane.

[0015] Preferably, the pore size of the DNA enrichment membrane ranges from 0.22 to 0.45 µm.

[0016] Compared with the prior art, the device has the following beneficial effects:

[0017] 1. The device for actively collecting environmental DNA based on a UAV, by arranging the active enrichment power unit and the sample enrichment unit, the DNA enrichment membrane is loaded on the UAV body through the sample enrichment unit, the electric pump of the active enrichment power unit rapidly adsorbs the surrounding air, the air passes through the DNA enrichment membrane on the sample enrichment unit, the UAV body drives the air close to the air above various ecosystems to collect environmental DNA samples, and more biological information is rapidly collected through the active enrichment power unit, thereby solving the problem that the DNA content in the air is too small to be enriched and improving the efficiency of biological DNA collection.

[0018] 2. The unmanned aerial vehicle-mounted environmental DNA active collection device, by setting the protective cover and the moving assembly, after the sample enrichment unit collects the environmental DNA in the specified area, the protective cover covers the layout plate, the DNA enrichment film is covered, and the sample on the DNA enrichment film is prevented from being contaminated when the unmanned aerial vehicle body drives the sample enrichment unit to fly back. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a three-dimensional structure schematic diagram of the utility model;

[0020] Figure 2 It is a side view structure schematic diagram of the utility model;

[0021] Figure 3 It is a protective cover structure schematic diagram of the utility model;

[0022] Figure 4 It is a moving assembly position structure schematic diagram of the utility model;

[0023] Figure 5 It is a card plate position structure schematic diagram of the utility model.

[0024] In the drawing: 1, unmanned aerial vehicle body; 2, active enrichment power unit; 21, electric pump; 22, air duct; 3, sample enrichment unit; 31, installation box; 32, layout plate; 33, DNA enrichment film; 4, protective cover; 5, moving assembly; 51, linear motor; 52, connecting plate; 6, guide rail; 7, guide block; 8, adapter frame; 9, card plate; 10, groove; 11, protruding block; 12, support frame. DETAILED DESCRIPTION

[0025] The technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0026] Please refer to Figures 1-5 An unmanned aerial vehicle-mounted environmental DNA active collection device, comprising: an active enrichment power unit 2 and a sample enrichment unit 3 arranged at the bottom of the unmanned aerial vehicle body 1, the active enrichment power unit 2 being connected to one end of the sample enrichment unit 3.

[0027] The active enrichment power unit 2 comprises an electric pump 21 and an air duct 22, the electric pump 21 being installed at the bottom of the unmanned aerial vehicle body 1, and the air duct 22 being installed at the air inlet end of the electric pump 21.

[0028] The sample enrichment unit 3 comprises a mounting box 31 arranged at the bottom of the unmanned aerial vehicle body 1, the surface of the mounting box 31 is connected with one end of the air duct 22, a layout plate 32 is arranged on the mounting box 31, and at least one DNA enrichment membrane 33 is arranged on the layout plate 32.

[0029] In a specific embodiment, the surface of the mounting box 31 is connected with the protective cover 4, the surface of the mounting box 31 away from the layout plate 32 is provided with a moving assembly 5, the moving assembly 5 comprises a linear motor 51, a connecting plate 52 is arranged on the moving block of the linear motor 51, the surface of the connecting plate 52 is connected with the surface of the protective cover 4, the surface of the mounting box 31 is connected with a guide rail 6, the surface of the guide rail 6 is connected with a guide block 7, the surface of the guide block 7 is connected with a connecting frame 8, the surface of the connecting frame 8 is connected with the surface of the protective cover 4, after the sample enrichment unit 3 collects the environmental DNA in the designated area, the linear motor 51 is driven, the moving block of the linear motor 51 drives the connecting plate 52 to move, the connecting plate 52 drives the protective cover to move, the layout plate 32 is covered by the protective cover, the DNA enrichment membrane 433 is covered, and the sample on the DNA enrichment membrane 433 is prevented from being polluted when the unmanned aerial vehicle body 1 drives the sample enrichment unit 3 to fly back.

[0030] The top and bottom of the layout plate 32 are provided with clamping plates 9, recesses 10 are formed in the surface of the clamping plates 9, the surface of the mounting box 31 is provided with protrusions 11 corresponding to the positions of the recesses 10, and the protrusions 11 are clamped on the inner walls of the recesses 10.

[0031] The DNA enrichment membrane 33 is made of nitrocellulose membrane, polycarbonate membrane or glass fiber membrane, and the pore size of the DNA enrichment membrane 33 ranges from 0.22 to 0.45 µm.

[0032] The layout plate 32 on which the DNA enrichment membrane 433 is arranged is mounted on the mounting box 31, the mounting box 31 is mounted on the bottom of the unmanned aerial vehicle body 1 by using the support frame 12, the unmanned aerial vehicle body 1 carries the DNA enrichment membrane 433 to fly to the collection area, the air suction electric pump 21 is started under remote control, the electric pump 21 quickly absorbs the surrounding air, the air passes through the DNA enrichment membrane 433 on the layout plate 32, the unmanned aerial vehicle body 1 collects the environmental DNA sample in the air close to various ecological systems, and more biological information is collected by the active enrichment power unit 2, so that the problem that the DNA content in the air is too small to be enriched is solved, and the efficiency of biological DNA collection is improved.

[0033] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An environmental DNA active collection device based on a drone, characterized in that, The application relates to an unmanned aerial vehicle for enriching DNA, which comprises the following parts: a main active enrichment power unit (2) and a sample enrichment unit (3) arranged at the bottom of an unmanned aerial vehicle body (1), wherein the main active enrichment power unit (2) is connected to one end of the sample enrichment unit (3); the main active enrichment power unit (2) comprises an electric pump (21) and a ventilation pipeline (22), wherein the electric pump (21) is arranged at the bottom of the unmanned aerial vehicle body (1), and the ventilation pipeline (22) is arranged at the air inlet end of the electric pump (21); the sample enrichment unit (3) comprises a mounting box (31) arranged at the bottom of the unmanned aerial vehicle body (1), wherein the surface of the mounting box (31) is connected to one end of the ventilation pipeline (22), a layout plate (32) is arranged on the mounting box (31), and at least one DNA enrichment membrane (33) is arranged on the layout plate (32).

2. The UAV-mounted environmental DNA active collection device of claim 1, wherein, The surface of the mounting box (31) is connected to a protective cover (4), and the surface of the mounting box (31) away from the layout plate (32) is provided with a moving assembly (5).

3. The UAV-mounted environmental DNA active collection device of claim 2, wherein, The moving assembly (5) comprises a linear motor (51), and a connecting plate (52) is arranged on the moving block of the linear motor (51); the surface of the connecting plate (52) is connected to the surface of the protective cover (4).

4. The UAV-mounted environmental DNA active collection device of claim 3, wherein, The protective cover (4) covers the layout plate (32).

5. The UAV-mounted environmental DNA active collection device of claim 3, wherein, The surface of the mounting box (31) is connected to a guide rail (6), the surface of the guide rail (6) is connected to a guide block (7), the surface of the guide block (7) is connected to a connecting frame (8), and the surface of the connecting frame (8) is connected to the surface of the protective cover (4).

6. The UAV-mounted environmental DNA active collection device of claim 1, wherein, The top and bottom of the layout plate (32) are provided with clamping plates (9), the surface of the clamping plate (9) is provided with a groove (10), the surface of the mounting box (31) is provided with a convex block (11) corresponding to the position of the groove (10), and the convex block (11) is clamped on the inner wall of the groove (10).

7. The UAV-mounted environmental DNA active collection device of claim 1, wherein, The surface of the mounting box (31) is provided with a supporting frame (12), and the supporting frame (12) is arranged at the bottom of the unmanned aerial vehicle.

8. The UAV-mounted environmental DNA active collection device of claim 1, wherein, The DNA enrichment membrane (33) is made of nitrocellulose membrane, polycarbonate membrane or glass fiber membrane material.

9. The UAV-mounted environmental DNA active collection device of claim 1, wherein, The pore size of the DNA enrichment membrane (33) ranges from 0.22 to 0.45 mu m.

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