Fluorescence detection device

Abstract

The utility model discloses a fluorescence detection device which is used for collecting fluorescence signals on a sample film, the fluorescence detection device comprises an excitation light source, a detection element and a camera obscura, the excitation light source and the detection element are both arranged in the camera obscura, the excitation light source acts on the sample film to excite the sample film to generate the fluorescence signals, the detection element is located below the sample film, and the detection element is located below the sample film. The detector is used for detecting a fluorescence signal and converting the fluorescence signal into an electric signal; the fluorescence detection device further comprises a first filtering element, and the first filtering element is arranged above the sample film, is positioned below the excitation light source and is used for filtering interference light emitted by the excitation light source; and / or the fluorescence detection device further comprises a second filter element, the second filter element is arranged below the sample membrane, and the second filter element is located above the detection element and used for filtering interference light reaching the detection element; wherein the second filter element is a micro optical fiber plate.

Description

TECHNICAL FIELD

[0001] The utility model relates to a fluorescence detection device. BACKGROUND

[0002] The fluorescence label is widely used in the detection of the marker in the biological sample, and the fluorescence signal generated by collecting the sample is used to detect the distribution or content of the relevant marker substance. The fluorescence signal is generated by irradiating the excitation light to the biological sample with the fluorescence label, and then the signal is detected by the detection element and transmitted to the computer, so that the color image is displayed and quantitative analysis is carried out.

[0003] The detection of the traditional fluorescence label sample signal mainly adopts the optical imaging mode of the digital camera and the optical lens to collect and analyze the fluorescence signal, and mainly includes the digital camera, the optical lens, the excitation light module, the excitation light source filter, the reflected light filter and other main components. The traditional optical imaging mode, because there is a large distance between the digital camera and the lens and the biological sample, the digital camera can only collect a small part of the fluorescence signal emitted by the sample, so that the detection of the weak light signal is limited to a certain extent. UTILITY MODEL CONTENT

[0004] The utility model solves the technical problem that the digital camera can only collect a small part of the fluorescence signal emitted by the sample in the prior art, and provides a fluorescence detection device.

[0005] The utility model solves the above technical problem by the following technical scheme:

[0006] The utility model discloses a fluorescence detection device for collecting the fluorescence signal on the sample film, the fluorescence detection device includes excitation light source, detection element and dark box, the excitation light source and the detection element all are located in the dark box, the excitation light source acts on the sample film, to excite the fluorescence signal on the sample film, the detection element is located below the sample film, is used to detect the fluorescence signal and converts the fluorescence signal into the electric signal;

[0007] The fluorescence detection device further includes a first filter element, the first filter element is arranged above the sample film, and the first filter element is located below the excitation light source, and is used to filter the interference light emitted by the excitation light source.

[0008] And / or, the fluorescence detection device further includes a second filter element, the second filter element is arranged below the sample film, and the second filter element is located above the detection element, and is used to filter the interference light reaching the detection element, wherein the second filter element is a micro optical fiber plate.

[0009] In the scheme, the above structure is adopted, the dark box is arranged, the light shielding environment is provided for the excitation of the sample film by the excitation light source to generate silver light signal and the detection of the influence signal, the external light interference is effectively avoided, and the accuracy of the detection result is ensured. The excitation light source is located above the sample film, so that the fluorescent substance on the sample film can be excited by the excitation light source to generate a signal. The detection element is located below the sample film and is arranged in close proximity, so that the detection element can capture the fluorescent signal emitted by the sample film, reduce the loss of the signal in the transmission process, and improve the detection efficiency and accuracy. The first filtering element is arranged below the laser element and above the sample film, can filter the interference light emitted by the excitation light source, and avoid the adverse effects of stray light on the fluorescent signal. The second filtering element is a micro optical fiber plate, which is located above the detection element and below the sample film, and performs secondary filtering before the light is transmitted to the detection element, further eliminates the residual interference light, and relies on the special optical transmission and filtering characteristics of the micro optical fiber plate to not only screen out the fluorescent wave band meeting the detection requirements, but also enhance the transmission stability of the light signal, so that the fluorescent signal received by the final detection element is not only pure but also stable, thereby greatly improving the detection accuracy and reliability of the entire fluorescent detection device.

[0010] Preferably, the thickness of the micro optical fiber plate is 1mm-5mm;

[0011] And / or, the optical fiber diameter of the micro optical fiber plate is 3-20 microns.

[0012] In the scheme, the above structure is adopted, the micro optical fiber plate can effectively control the scattering and loss of light in the transmission process while maintaining its own structural stability, and the intensity of the fluorescent signal transmitted to the detection element is improved.

[0013] The light diameter of the micro optical fiber plate is 3-20 microns, which can improve the spatial resolution, so that the detection element can more accurately detect the fluorescent signal change of a small area on the sample film.

[0014] Preferably, the detection element includes a CMOS detector.

[0015] Preferably, the size of the CMOS detector is 3cm*3cm-15cm*15cm.

[0016] And / or, the pixel size of the CMOS detector is 20 microns*20 microns-100 microns*100 microns.

[0017] Preferably, the fluorescent detection device further comprises an expansion element, which is arranged between the sample film and the first filtering element, and is used for diffusing the light filtered by the first filtering element into a surface light source.

[0018] In this scheme, the above-mentioned structure is adopted, and the beam expanding element is located between the sample film and the first filtering element. The light filtered by the first filtering element can be diffused to convert it into a uniform surface light source, thereby effectively solving the problem of uneven light spot and ensuring that each area on the sample film can uniformly receive excitation light, thereby improving the excitation efficiency and uniformity of the fluorescence signal. The uniform surface light source helps to reduce signal fluctuations and errors during detection, so that the detection element can more stably and accurately capture the fluorescence signal, improving the reliability and repeatability of the entire detection device. In addition, the application of the beam expanding element can also expand the coverage of the excitation light, so that when performing fluorescence detection on a larger area of the sample film, the signal intensity of each detection point can still be ensured to be consistent, which can significantly improve the detection efficiency and the accuracy of the data. At the same time, the uniform diffusion of light also helps to optimize the working conditions of the detection element, avoiding the problems of detection element saturation or weak signal caused by local light intensity being too strong or too weak, further improving the sensitivity and dynamic range of detection.

[0019] Preferably, the diffusion angle of the light after passing through the beam expanding element is 5°-30°.

[0020] Preferably, the outer edge of the beam expanding element coincides with the outer edge of the light sensing area of the detection element.

[0021] Alternatively, the outer edge of the beam expanding element is located outside the outer edge of the light sensing area of the detection element.

[0022] In this scheme, when the outer edge of the beam expanding element coincides with the outer edge of the light sensing area of the detection element, the surface light source after beam expansion can accurately match the light sensing area of the detection element, ensuring that the entire light sensing area uniformly receives the filtered and diffused excitation light, avoiding light overflow or deficiency, so that the detection element can efficiently capture the fluorescence signal generated on the sample film, improving the accuracy and stability of detection. When the outer edge of the beam expanding element is located outside the outer edge of the light sensing area of the detection element, the coverage of the excitation light can be further expanded, ensuring that even in the edge area of the light sensing area of the detection element, sufficient intensity and uniform excitation light can be received. This is particularly advantageous for the case where the fluorescence substance on the sample film is unevenly distributed or the light sensing area of the detection element is slightly smaller than the beam expanding element, which can effectively reduce the detection errors caused by uneven light intensity and improve the uniformity and reliability of detection.

[0023] Preferably, the fluorescence detection device further comprises a condensing element, which is arranged between the excitation light source and the first filtering element and is used to converge the excitation light source into a light beam.

[0024] In the scheme, the light emitted by the excitation light source can pass through the first filtering element better, so that the first filtering element can filter the interference light and reduce the influence of the interference light on the sample film.

[0025] Preferably, the second filtering element is one or more of a single-channel optical filter, a single-channel band-pass optical filter, and a multi-position optical filter.

[0026] Preferably, the excitation light source is one or more of an LED lamp, a deuterium lamp, a halogen lamp, a solid-state laser emitter, or a gaseous laser emitter.

[0027] The positive progress effect of the utility model lies in:

[0028] The dark box provides a light-shielded environment for the excitation light source to excite the sample film to generate silver light signals and for the detection element to detect influence signals, effectively avoids external light interference, and ensures the accuracy of the detection results. The excitation light source is located above the sample film, so that the excitation light source can excite the fluorescent substance on the sample film to generate signals. The detection element is located below the sample film and is arranged at a close distance, so that the detection element can capture the fluorescent signals emitted by the sample film, reduces the loss of signals in the transmission process, and improves the detection efficiency and accuracy. The first filtering element is arranged below the laser element and above the sample film, can filter the interference light emitted by the excitation light source, and avoids the adverse effects of stray light on the fluorescent signals. The second filtering element is a micro optical fiber plate, is located above the detection element and below the sample film, and performs secondary filtering before the light is transmitted to the detection element, further eliminates residual interference light, and relies on the special optical transmission and filtering characteristics of the micro optical fiber plate to not only screen out fluorescent wavebands that meet the detection requirements, but also enhance the transmission stability of the light signals, so that the fluorescent signals received by the final detection element are pure and stable, thereby greatly improving the detection accuracy and reliability of the entire fluorescent detection device. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 FIG. 1 is a structural schematic diagram of a fluorescent detection device according to an embodiment of the utility model.

[0030] Figure 2 FIG. 2 is a cross-sectional structural schematic diagram of the fluorescent detection device according to the embodiment of the utility model.

[0031] Figure 3 FIG. 3 is a partial cross-sectional structural schematic diagram of the fluorescent detection device according to the embodiment of the utility model.

[0032] Figure 4 FIG. 4 is a partial cross-sectional schematic diagram of the fluorescent detection device according to the embodiment of the utility model.

[0033] BRIEF DESCRIPTION OF DRAWINGS

[0034] Fluorescence detection device 100

[0035] Laser power supply 1

[0036] Detection element 2

[0037] Dark box 3

[0038] First filter element 4

[0039] Second filter element 5

[0040] Beam expanding element 6

[0041] Light collecting element 7

[0042] Sample stage 8

[0043] Detection area 81 DETAILED DESCRIPTION

[0044] The utility model is further illustrated below by way of examples, but the utility model is not limited in the scope of the examples.

[0045] As Figures 1 to 4 shown, the embodiment provides a fluorescence detection device for collecting fluorescence signal on sample film, the fluorescence detection device includes excitation light source, detection element and dark box, the excitation light source and detection element are located in the dark box, the excitation light source acts on sample film to excite fluorescence signal on sample film, and the detection element is located below sample film and is used to detect fluorescence signal and convert fluorescence signal into electric signal;The fluorescence detection device further includes first filter element and second filter element, for the first filter element and the second filter element, there can be the following several implementation manners, the first implementation manner, the first filter element is located above sample film, and the first filter element is located below the excitation light source and is used to filter interference light emitted by the excitation light source;The second implementation manner, the second filter element is located below sample film, and the second filter element is located above the detection element and is used to filter interference light reaching the detection element;Wherein the second filter element is micro optical fiber board;The third implementation manner, the first filter element is located above sample film, and the first filter element is located below the excitation light source and is used to filter interference light emitted by the excitation light source, and the second filter element is located below sample film, and the second filter element is located above the detection element and is used to filter interference light reaching the detection element;Wherein the second filter element is micro optical fiber board.

[0046] Preferably, the first filter element is arranged above the sample membrane and below the excitation light source to filter the interference light emitted by the excitation light source, and the second filter element is arranged below the sample membrane and above the detection element to filter the interference light reaching the detection element; wherein the second filter element is a micro-fiber plate. With the above structure, by arranging the dark box, the excitation light source excites the sample membrane to generate silver light signals and the detection element detects the signals in a light-shielded environment, effectively avoiding external light interference and ensuring the accuracy of the detection results. The excitation light source is arranged above the sample membrane, so that the excitation light source can excite the fluorescent substance on the sample membrane to generate signals. The detection element is arranged below the sample membrane and in close proximity, so that the detection element can capture the fluorescent signals emitted by the sample membrane, reducing the loss of signals in the transmission process and improving the detection efficiency and accuracy. The first filter element is arranged below the laser element and above the sample membrane, which can filter the interference light emitted by the excitation light source and avoid the adverse effects of stray light on the fluorescent signals. The second filter element is a micro-fiber plate arranged above the detection element and below the sample membrane, which can further filter the residual interference light before the light reaches the detection element. By virtue of the special optical transmission and filtering characteristics of the micro-fiber plate, not only can the fluorescent waveband meeting the detection requirements be selected, but also the transmission stability of the light signal can be enhanced, so that the fluorescent signal received by the final detection element is pure and stable, thereby greatly improving the detection accuracy and reliability of the entire fluorescent detection device.

[0047] It should be specifically pointed out that the above-mentioned upper refers to the direction opposite to the illumination direction of the excitation light source; the above-mentioned lower refers to the illumination direction of the excitation light source.

[0048] In specific use, the micro-fiber plate is a specific wavelength filter after multi-layer sputtering coating treatment; in other embodiments, the micro-fiber plate can also be a market-purchased finished product.

[0049] In addition, as shown in Figure 2 and Figure 4 , the fluorescent detection device further comprises a sample stage, and the biological sample membrane is placed on the detection area of the sample stage. After the light passes through the diffusion element, it can cover at least the detection area.

[0050] In biological membrane detection, the dark box refers to a specially designed closed light-shielded device or experimental environment, and its core function is to completely isolate external light interference to ensure the stability of photosensitive reactions or optical signals during detection. The dark box is usually made of light-proof materials such as black metal, light-shielded coated plastic or sealed light-shielded cloth.

[0051] For the micro-fiber plate, the thickness can be 1mm-5mm; with the above structure, the micro-fiber plate can effectively control the scattering and loss of light in the transmission process while maintaining its own structural stability, thereby improving the intensity of the fluorescent signal transmitted to the detection element. In other embodiments, the thickness of the micro-fiber plate can be adjusted according to actual needs, which is not limited herein.

[0052] The fiber diameter of the micro-fiber plate is 3-20 microns, which can improve the spatial resolution, so that the detection element can more accurately detect the fluorescent signal changes of the micro area on the sample film. In other embodiments, the fiber diameter of the micro-fiber plate can be adjusted according to actual needs, which is not limited herein.

[0053] In this embodiment, the detection element includes a CMOS detector, and in other embodiments, the detection element can also adopt other forms, which is not limited herein.

[0054] For the CMOS detector, the size can be 3cm×3cm-15cm×15cm, and the pixel size is 20 microns×20 microns-100 microns×100 microns.

[0055] As shown in Figure 2 and Figure 3 , the fluorescence detection device further comprises a beam expander element, which is arranged between the sample film and the first filter element, and is used for diffusing the light filtered by the first filter element into a surface light source. With the above structure, the beam expander element is located between the sample film and the first filter element, which can diffuse the light filtered by the first filter element, so that it is converted into a uniform surface light source, thereby effectively solving the problem of uneven light spot, ensuring that each area on the sample film can uniformly receive the excitation light, thereby improving the excitation efficiency and uniformity of the fluorescent signal. The uniform surface light source helps to reduce signal fluctuations and errors during detection, so that the detection element can more stably and accurately capture the fluorescent signal, thereby improving the reliability and repeatability of the entire detection device. In addition, the application of the beam expander element can also expand the coverage range of the excitation light, so that when performing fluorescent detection on a larger area of the sample film, the signal intensity of each detection point can still be ensured to be consistent, which can significantly improve the detection efficiency and the accuracy of the data. At the same time, this uniform diffusion of light also helps to optimize the working conditions of the detection element, avoiding the problems of detection element saturation or weak signal caused by local light intensity being too strong or too weak, thereby further improving the sensitivity and dynamic range of detection.

[0056] In this embodiment, the diffusion angle of the light after the beam expander element is 5°-30°. In other embodiments, the diffusion angle of the light can be adjusted according to actual needs, which is not limited herein.

[0057] The outer edge of the beam expanding element coincides with the outer edge of the light sensing area of the detecting element, or the outer edge of the beam expanding element is located outside the outer edge of the light sensing area of the detecting element. With the above structure, when the outer edge of the beam expanding element coincides with the outer edge of the light sensing area of the detecting element, the expanded surface light source can accurately match the light sensing area of the detecting element, ensuring that the entire light sensing area uniformly receives the filtered and diffused excitation light, avoiding light overflow or deficiency, so that the detecting element can efficiently capture the fluorescent signal generated on the sample film, improving the accuracy and stability of detection. When the outer edge of the beam expanding element is located outside the outer edge of the light sensing area of the detecting element, the coverage of the excitation light can be further expanded, ensuring that even in the edge area of the light sensing area of the detecting element, sufficient intensity and uniform excitation light can be received. This is particularly advantageous for the case where the fluorescent substance on the sample film is unevenly distributed or the light sensing area of the detecting element is slightly smaller than the beam expanding element, effectively reducing the detection error caused by uneven light intensity and improving the uniformity and reliability of detection.

[0058] As shown in Figure 2 and Figure 3 The fluorescence detection device further comprises a condensing element, which is arranged between the excitation light source and the first filtering element and is used for converging the excitation light source into a light beam. With the above structure, the light emitted by the excitation light source can pass through the first filtering element better, so that the first filtering element can filter out the interference light and reduce the influence of the interference light on the sample film.

[0059] In this embodiment, the second filtering element is one or more of a single-channel filter, a single-channel band-pass filter, and a multi-position filter.

[0060] The excitation light source is one or more of an LED lamp, a deuterium lamp, a halogen lamp, a solid-state laser emitter, or a gaseous laser emitter.

[0061] Although the specific embodiments of the present application are described above, those skilled in the art should understand that this is only an example, and the protection scope of the present application is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present application, and these changes and modifications all fall within the protection scope of the present application.

Claims

1. A fluorescence detection device for collecting a fluorescence signal on a sample membrane, characterized in that, The fluorescence detection device comprises an excitation light source, a detection element and a dark box, the excitation light source and the detection element are arranged in the dark box, the excitation light source acts on the sample film to excite the sample film to generate a fluorescence signal, and the detection element is located below the sample film and is used for detecting the fluorescence signal and converting the fluorescence signal into an electric signal. The fluorescence detection device further comprises a first filtering element, the first filtering element is arranged above the sample film, and the first filtering element is located below the excitation light source and is used for filtering interference light emitted by the excitation light source. And / or, the fluorescence detection device further comprises a second filtering element, the second filtering element is arranged below the sample film, and the second filtering element is located above the detection element and is used for filtering interference light reaching the detection element; wherein the second filtering element is a micro optical fiber plate.

2. The fluorescence detection apparatus of claim 1, wherein The thickness of the micro optical fiber plate is 1mm-5mm. And / or, the diameter of the optical fiber of the micro optical fiber plate is 3 microns-20 microns.

3. The fluorescence detection apparatus of claim 1, wherein The detection element comprises a CMOS detector.

4. The fluorescence detection apparatus of claim 3, wherein The size of the CMOS detector is 3cm×3cm-15cm×15cm. And / or, the pixel size of the CMOS detector is 20 microns×20 microns-100 microns×100 microns.

5. The fluorescence detection apparatus of claim 1, wherein The fluorescence detection device further comprises a beam expanding element, the beam expanding element is arranged between the sample film and the first filtering element, and is used for diffusing the light filtered by the first filtering element into a surface light source.

6. The fluorescence detection apparatus of claim 5, wherein The diffusion angle of the light after the beam expanding element is 5°-30°.

7. The fluorescence detection apparatus of claim 5, wherein The outer edge of the beam expanding element coincides with the outer edge of the light sensing area of the detection element. Or, the outer edge of the beam expanding element is located outside the outer edge of the light sensing area of the detection element.

8. The fluorescence detection apparatus of claim 1, wherein, The fluorescence detection device further comprises a light collecting element, the light collecting element is arranged between the excitation light source and the first filtering element, and is used for converging the excitation light source into a light beam.

9. The fluorescence detection apparatus of claim 1, wherein, The second filtering element is one or more of a single-channel optical filter, a single-channel band-pass optical filter and a multi-position optical filter.

10. The fluorescence detection device of any one of claims 1-9, wherein, The excitation light source is one or more of an LED lamp, a deuterium lamp, a halogen lamp, a solid-state laser emitter or a gaseous laser emitter.

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