Integrated anti-overflow sterile filter device for virus culture

Abstract

The utility model discloses an integrated spill-proof sterile filter device for virus culture, which comprises a piston tube, a connecting tube, an aluminum film, a filter, a puncture piece, a liquid suction tube, a spherical valve and a thin plastic tube, the connecting tube is arranged at the front end of the piston tube, and the orifice of the connecting tube is sealed by the aluminum film; the filter is detachably arranged on the connecting pipe; a puncturing piece is arranged in a pipe opening, connected with the connecting pipe, of the filter; a liquid suction pipe is arranged on the side face of the front end of the piston pipe, a spherical valve is arranged on the liquid suction pipe, and the thin plastic pipe is arranged at the front end of the liquid suction pipe in a sealed and inserted mode. According to the utility model, the filter sucks and filters a virus sample by adopting a'double-channel 'arrangement, a needle head and the filter do not need to be assembled and disassembled for multiple times in the operation process, and a plastic thin tube is adopted for sucking during liquid suction, so that the use of different virus sample containers can be met.

Description

Technical Field

[0001] This utility model belongs to the field of biological experimental equipment technology, and specifically relates to an integrated anti-overflow sterile filter device for virus culture. Background Technology

[0002] In virus culture experiments, the filtration of samples (or cultures, etc.) typically uses traditional needle filters, requiring repeated loading and unloading of the syringe needle and manual connection of the filter. This method has the following drawbacks: 1. Complex operation: Multiple loading and unloading of the needle and filter is cumbersome and prone to contamination; 2. Sharps risk: Syringe needles are metal sharp objects, posing a risk of puncture wounds and biological exposure; 3. Liquid spillage: If the connection between the needle and filter is not tight, excessive injection pressure can cause the virus sample to spray out; 4. Low efficiency: Multiple operations are required for a single filtration, making it difficult to meet the demands of high-throughput experiments; 5. Inability to achieve precise filtration: Sample liquid residue remains in the filter after filtration. Therefore, a new type of filter is needed for the filtration of samples (or cultures, etc.) in virus culture experiments. Utility Model Content

[0003] In view of the shortcomings of the prior art, this invention provides an integrated anti-overflow sterile filter device for virus culture.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] An integrated, spill-proof sterile filter device for virus culture includes a piston tube, a connecting tube, an aluminum membrane, a filter, a puncture disc, a suction tube, a ball valve, and a thin plastic tube. The piston tube has a connecting tube at its front end, and the opening of the connecting tube is sealed with an aluminum membrane. The filter is detachably mounted on the connecting tube. A puncture disc is installed inside the opening where the filter connects to the connecting tube. The piston tube has a suction tube at its front end, and a ball valve is installed on the suction tube. The thin plastic tube is sealed and inserted into the front end of the suction tube.

[0006] The filter of this invention adopts a "dual-channel" setting for both aspirating and filtering virus samples. During operation, it is not necessary to repeatedly attach and detach the needle and filter. The filter uses a thin plastic tube for aspiration, which can meet the needs of different virus sample containers.

[0007] Furthermore, the front end of the piston tube is tapered, which prevents virus samples from remaining inside the piston tube.

[0008] Furthermore, the connection between the connecting pipe and the filter is a threaded connection, which can withstand excessive injection pressure and prevent the virus sample from overflowing during the filtration process.

[0009] Furthermore, the filter membrane has a pore size of 0.22 μm. Since the diameter of viruses is mostly 0.02-0.3 μm and the diameter of bacteria is mostly 0.2-2 μm, the filter with a pore size of 0.22 μm can trap most bacteria, yeast, mold, and large particles, allowing only viruses to pass through, thus achieving the purpose of filtration.

[0010] Furthermore, the piston tube has graduations on its side, allowing for direct observation of the amount of filtered virus samples.

[0011] The advantages of this utility model are as follows: 1. The piston tube, suction tube, and filter structure of the filtration device of this utility model are integrated, and the suction-filtration-discharge are completed in a single action. There is no need to disassemble and replace the filter or needle during the filtration operation, which reduces the time by more than 60%; 2. The piston tube and the filter are connected by threads, and the suction tube is sealed with a ball valve. The double anti-overflow design ensures the stability of high-pressure injection; 3. The overall design is free of sharps, eliminating the risk of punctures and meeting the requirements of biosafety level 2 (BSL-2) laboratories; 4. After the virus sample in the piston tube is filtered, air can be drawn in again to completely filter the virus sample in the filter, achieving precision filtration; 5. The overall structure is simple, the production cost is low, and it is suitable for large-scale application. Attached Figure Description

[0012] Figure 1 This is a perspective view of an integrated anti-overflow sterile filter device for virus culture according to this utility model;

[0013] Figure 2 This is a front view of an integrated anti-overflow sterile filter device for virus culture according to this utility model;

[0014] Figure 3 This is a perspective view of the piston tube in an integrated anti-overflow sterile filter device for virus culture according to this utility model;

[0015] Figure 4 This is a perspective view of the filter in an integrated anti-overflow sterile filter device for virus culture according to this utility model. Detailed Implementation

[0016] The present invention will now be described in detail with reference to the accompanying drawings:

[0017] In the description of this utility model, it should be noted that the orientation or positional relationship indicated by "front", "rear", etc. is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the utility model product is usually placed in during use. It is only for the convenience of describing this utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, or a specific orientation structure and operation. Therefore, it should not be construed as a limitation of this utility model.

[0018] like Figure 1-4 As shown, an integrated anti-overflow sterile filter device for virus culture includes a piston tube 1, a connecting tube 2, an aluminum membrane 3, a filter 4, a puncture disc 5, a suction tube 6, a ball valve 7, and a plastic capillary tube 8. The entire device employs a sharps-free design and is made of medical-grade polypropylene that is chemically inert, highly transparent, and resistant to organic solvents and biological virus samples. The piston tube 1 has a tapered front end, which prevents virus samples from remaining inside the tube after filtration. Graduation marks are also provided on the piston tube 1. The connecting tube 2 is located at the front end of the piston tube 1, and its opening is sealed with an aluminum membrane 3. The aluminum membrane 3 ensures that only the suction tube 6 is open during the suction process. The filter 4 is detachably mounted on the connecting tube 2, and the two are connected by threads. The threaded connection can withstand excessive injection pressure and provides a good seal, preventing virus samples from overflowing during filtration. A puncture disc 5 is installed inside the port where the filter 4 connects to the connecting pipe 2. The front end of the puncture disc 5 is not exposed. The function of the puncture disc 5 is to puncture the aluminum membrane 3 during the installation of the filter 4. A suction tube 6 is installed at the front end of the piston tube 1, specifically on the side of the front end of the piston tube 1. A ball valve 7 is installed on the suction tube 6, which can control the opening and closing of the suction tube 6, and the sealing effect meets the experimental requirements. A plastic thin tube 8 is sealed and inserted at the front end of the suction tube 6. The inner wall of the suction tube 6 is provided with a rubber layer, and the tube diameter is slightly smaller than the diameter of the plastic thin tube 8. This ensures that the plastic thin tube 8 can meet the requirements of connection and sealing after insertion. The plastic thin tube 8 is provided with various lengths, and a suitable plastic thin tube 8 can be selected to be inserted into different containers to draw virus samples during use.

[0019] The filter 4 in this embodiment uses a needle filter with a pore size of 0.22 μm, which can meet the filtration requirements of different types of virus samples.

[0020] Operating principle: 1. Insert the plastic capillary tube 8 into the aspiration tube 6 and open the ball valve 7; 2. Insert the plastic capillary tube 8 into the container holding the virus sample and operate the piston tube 1 to aspirate the virus sample according to the required filtration volume, then close the ball valve 7; 3. Install the filter 4 and filter the virus sample according to the experimental requirements. After filtration, discard the entire filtration device in the sharps container for further processing. In some highly pathogenic pathogen culture or detection processes, precise virus sample filtration is required. Control the aspiration volume during aspiration, and after filtration, open the ball valve 7 to open the piston tube 1 to draw in clean air, then close the ball valve 7 to completely filter the residual virus sample in the filter 4, achieving precise filtration.

[0021] Although the specific embodiments of this utility model have been described and explained in detail above, it should be noted that we can make various changes and modifications to the above embodiments, but these will not depart from the spirit of this utility model and the scope set forth in the appended claims.

Claims

1. An integrated anti-overflow sterile filter device for virus culture, characterized in that, The device includes a piston tube (1), a connecting tube (2), an aluminum membrane (3), a filter (4), a puncture plate (5), a suction tube (6), a ball valve (7), and a plastic thin tube (8). The piston tube (1) is provided with a connecting tube (2) at its front end, and the opening of the connecting tube (2) is sealed with an aluminum membrane (3). The filter (4) is detachably mounted on the connecting tube (2). A puncture plate (5) is provided inside the opening where the filter (4) connects to the connecting tube (2). The piston tube (1) is provided with a suction tube (6) at its front end, and a ball valve (7) is provided on the suction tube (6). The plastic thin tube (8) is sealed and inserted into the front end of the suction tube (6).

2. The integrated anti-overflow sterile filter device for virus culture according to claim 1, characterized in that, The front end of the piston tube (1) is tapered.

3. The integrated anti-overflow sterile filter device for virus culture according to claim 2, characterized in that, The connection between the connecting pipe (2) and the filter (4) is a threaded connection.

4. The integrated anti-overflow sterile filter device for virus culture according to claim 1, characterized in that, The filter membrane of the filter (4) has a pore size of 0.22 μm.

5. The integrated anti-overflow sterile filter device for virus culture according to claim 1, characterized in that, The piston tube (1) has a scale on its side.

Images