Centrifugal machine for separating cells from culture medium

Abstract

The utility model provides a centrifugal machine for separating cells from a culture medium, and belongs to the technical field of centrifugal machines. Comprising a centrifugal machine base, a lower groove body is detachably arranged on the centrifugal machine base, an upper cover body is detachably arranged above the centrifugal machine base, a first connecting frame is horizontally arranged on the centrifugal machine base in a sliding mode, a second connecting frame is vertically arranged on the first connecting frame in a floating mode, and an upper groove body is movably arranged on the second connecting frame; a filter membrane is arranged on the side wall of the upper tank body, and a driving device which is connected with the upper tank body and drives the upper tank body to rotate is arranged on the upper cover body. The upper tank body and the lower tank body can be completely taken out of the centrifugal machine to be cleaned, and the effect of improving the operation convenience of the centrifugal machine is achieved.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge technology, and in particular to a centrifuge for separating cells and culture media. Background Technology

[0002] Centrifugation of cells in culture medium is a common operation in cell culture and experiments. Its main purpose is to enrich cells, remove impurities, or change culture conditions.

[0003] Patent CN211972321U discloses a stem cell conditioned medium purification device, including a box body. Inside the box body is a centrifugal filtration mechanism, with its top extending to the outer side of the top of the box body and its bottom extending to the outer side of the bottom of the box body. The centrifugal mechanism includes a motor, which is fixedly connected to the bottom of the box body. The motor drives the internal centrifugal filtration mechanism to centrifuge and separate the cell culture medium.

[0004] However, the device is complex, and the operation of removing cell clear liquid and turbid liquid after separating the culture medium is inconvenient. Cleaning the inner and outer layers of the centrifugation mechanism is also very inconvenient, which increases the difficulty of operating the instrument. Utility Model Content

[0005] In view of the above problems, the present invention provides a centrifuge for separating cells and culture medium.

[0006] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0007] A centrifuge for separating cells and culture medium is provided, including a centrifuge base, a lower tank detachably mounted on the centrifuge base, an upper cover detachably mounted above the centrifuge base, a first connecting frame horizontally slidably mounted on the centrifuge base, a second connecting frame vertically floating mounted on the first connecting frame, an upper tank movably mounted on the second connecting frame, a filter membrane mounted on the side wall of the upper tank, and a drive device mounted on the upper cover for connecting to the upper tank and driving the upper tank to rotate.

[0008] Furthermore, the driving device includes a connecting seat and a driving motor. The connecting seat is rotatably mounted on the bottom of the upper cover, and the driving motor is fixedly mounted on the upper cover and drivenly connected to the connecting seat. The connecting seat is provided with a connecting component for connecting to the upper groove.

[0009] Furthermore, the connecting assembly includes an airbag, which is coaxially disposed on the side wall of the connecting seat. A first air intake channel is coaxially provided on the connecting seat, which communicates with the airbag. A second air intake channel communicating with the first air intake is provided on the upper cover. A gas connector communicating with the second air intake channel is provided at the bottom of the upper cover. A gas connector seat for mating with the gas connector is provided on the lower tank. An air pump connected to the gas connector is provided inside the centrifuge base.

[0010] Furthermore, connecting protrusions are provided on the inner wall of the upper tank, and the connecting protrusions are spaced apart along the circumference of the upper tank.

[0011] Furthermore, the bottom of the upper cover is provided with an electrical connector, and the centrifuge base is provided with a waterproof connector that is compatible with the electrical connector.

[0012] Furthermore, a horizontal groove is provided on the outer wall of the centrifuge base, and the first connecting frame is damped and slidably mounted on the groove. The second connecting frame is floatingly connected to the first connecting frame through a telescopic rod. One end of the telescopic rod is connected to the first connecting frame and the other end is connected to the second connecting frame. A spring is provided inside the telescopic rod, and the telescopic rod is extended by the spring.

[0013] The beneficial effects of this utility model are as follows: by floating the upper tank in the lower tank, the upper tank is driven to rotate by the driving device on the upper cover to centrifuge the culture medium in the upper tank. After centrifugation, the upper cover can be removed to completely remove the upper tank. The turbid liquid obtained by centrifugation can be processed, while the clear liquid obtained through the semi-permeable membrane enters the lower tank. By sliding the first connecting frame to one side, the lower tank can be completely removed, allowing for a more thorough cleaning of the upper and lower tanks and improving the ease of operation of the centrifuge. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the centrifuge according to an embodiment of this application.

[0015] Figure 2 This is a schematic diagram of the exploded structure of a centrifuge according to an embodiment of this application.

[0016] Among them, 1. Centrifuge base; 11. Lower tank body; 12. Gas connector seat; 13. Waterproof connector seat; 14. Slide groove; 15. Telescopic rod; 2. Upper cover body; 21. Connecting seat; 22. Drive motor; 23. Airbag; 3. First connecting frame; 4. Second connecting frame; 5. Upper tank body; 51. Connecting protrusion. Detailed Implementation

[0017] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0018] This application discloses a centrifuge for separating cells and culture media, referring to... Figure 1 and Figure 2The centrifuge assembly includes a centrifuge base 1, an upper cover 2, a lower tank 11, and an upper tank 5. The upper cover 2 is detachably mounted on the centrifuge base 1 via a snap-fit ​​mechanism. The centrifuge base 1 has a mounting slot for accommodating the lower tank 11, which is movably mounted within the slot. A first connecting frame 3 is horizontally slidably mounted on the outer side of the centrifuge base 1. A second connecting frame 4 is located above the centrifuge base 1, and the second connecting frame 4 is sequentially floatingly connected to the first connecting frame 3. The second connecting frame 4 has mounting holes for the upper tank 5, and bearings are installed within these holes, allowing the upper tank 5 to rotate flexibly horizontally relative to the second connecting frame 4. A filter membrane is mounted on the side wall of the upper tank 5. The upper cover 2 has a drive device for connecting to and driving the upper tank 5 to rotate. When the upper cover 2 is closed on the centrifuge base 1, the drive device rotates the upper tank 5, allowing the culture medium within the upper tank 5 to be centrifuged.

[0019] Specifically, the drive device can be a connecting seat 21 and a drive motor 22. The connecting seat 21 is rotatably mounted on the bottom of the upper cover 2, and the drive motor 22 is fixedly mounted on the upper cover 2. The output shaft of the drive motor 22 drives the connecting seat 21 to rotate relative to the upper cover 2 through gears. The connecting seat 21 is provided with a connecting component for connecting or separating the connecting seat 21 from the upper tank 5. The connecting component can be an air bladder 23, which is coaxially arranged on the side wall of the connecting seat 21. When the upper cover 2 is closed and fastened to the centrifuge base 1, the connecting seat 21 extends into the upper tank 5, and the air bladder 23 faces the inner wall of the upper tank 5. A first air intake channel is provided inside the connecting seat 21, and a second air intake channel is provided inside the upper cover 2. One end of the first air intake channel is connected to the airbag 23, and the other end is coaxially opened at the top of the connecting seat 21. One end of the second air intake channel is connected to the first air intake channel, and the other end is connected to a vertically downward-facing gas connector. An upward-facing gas connector seat 12 is provided on the centrifuge base 1. When the upper cover 2 is closed and fastened to the centrifuge base 1, the gas connector can cooperate and communicate with the gas connector seat 12. An air pump connected to the gas connector seat 12 is provided inside the centrifuge base 1. By starting the air pump, air can be inflated into the airbag 23, and the airbag 23 applies pressure evenly to the upper tank 5, connecting the connecting seat 21 to the upper tank 5.

[0020] Furthermore, a connecting protrusion 51 is integrally formed on the inner wall of the upper tank 5. The connecting protrusion 51 is directly opposite the air bladder 23 on the connecting seat 21 when it is closed. Multiple connecting protrusions 51 are spaced apart along the circumference of the upper tank 5. The connecting protrusions 51 can improve the connection stability between the connecting seat 21 and the upper tank 5 when the air bladder 23 is squeezed against the inner wall of the upper tank 5, which is beneficial for the centrifugal separation of the culture medium in the upper tank 5. The spaced connecting protrusions 51 can facilitate the discharge of the turbid liquid obtained after centrifugation in the upper tank 5.

[0021] Furthermore, an electrical connector is provided at the bottom of the upper cover 2, and a waterproof connector 13 is provided on the centrifuge base 1. When the upper cover 2 is closed on the centrifuge base 1, the electrical connector is connected to the waterproof connector 13, which can supply power to the electrical components such as the drive motor 22 on the upper cover 2. The waterproof connector 13 can prevent liquid from entering the waterproof connector 13 during operation, thus preventing short circuits in the equipment.

[0022] Furthermore, a horizontal groove 14 is provided on the outer wall of the centrifuge base 1. The first connecting frame 3 is damped and slidably mounted on the groove 14. The second connecting frame 4 is floatingly connected to the first connecting frame 3 via a telescopic rod 15. One end of the telescopic rod 15 is connected to the first connecting frame 3, and the other end is connected to the second connecting frame 4. A spring is provided inside the telescopic rod 15, which drives the telescopic rod 15 to extend. A clearance groove is provided on the top wall of the centrifuge base 1 to accommodate the second connecting frame 4. When the first connecting frame 3 moves directly above the mounting groove and the second connecting frame 4 is pressed down by the upper cover 2, the second connecting frame 4 can fit into the clearance groove, thereby keeping the bottom wall of the upper cover 2 flat and in close contact with the centrifuge base 1. The telescopic rod 15 consists of an outer rod, an inner rod, and a spring. The outer rod is fixed to the first connecting frame 3. One end of the inner rod is fixedly connected to the second connecting frame 4, and the other end slides coaxially through the outer rod. The spring is disposed in the outer rod and is used to push the inner rod upward, thereby pushing the upper groove 5 above the mounting groove by the second connecting frame 4. This facilitates the movement of the first connecting frame 3 and the upper groove 5 to one side of the mounting groove, making it convenient for the installation and removal of the lower groove 11. Those skilled in the art will understand that although preferred embodiments of the present invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention. Obviously, those skilled in the art can make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and variations.

Claims

1. A cell and media separation centrifuge comprising: The centrifuge includes a centrifuge base (1), a lower tank (11) is detachably mounted on the centrifuge base (1), an upper cover (2) is detachably mounted on the top of the centrifuge base (1), a first connecting frame (3) is horizontally slidably mounted on the centrifuge base (1), a second connecting frame (4) is vertically floating mounted on the first connecting frame (3), an upper tank (5) is movably mounted on the second connecting frame (4), a filter membrane is mounted on the side wall of the upper tank (5), and a drive device is mounted on the upper cover (2) for connecting with the upper tank (5) and driving the upper tank (5) to rotate.

2. A centrifuge for separating cells and media according to claim 1, wherein, The driving device includes a connecting seat (21) and a driving motor (22). The connecting seat (21) is rotatably disposed at the bottom of the upper cover (2). The driving motor (22) is fixedly disposed on the upper cover (2) and is connected to the connecting seat (21) in a transmission manner. The connecting seat (21) is provided with a connecting component for connecting the upper groove (5).

3. A centrifuge for separating cells and media according to claim 2, wherein, The connecting assembly includes an airbag (23), which is coaxially disposed on the side wall of the connecting seat (21). A first air intake channel is coaxially provided on the connecting seat (21), which communicates with the airbag (23). A second air intake channel communicating with the first air intake is provided on the upper cover (2). A gas connector communicating with the second air intake channel is provided at the bottom of the upper cover (2). A gas connector seat (12) for cooperating with the gas connector is provided on the lower groove (11). An air pump connected to the gas connector is provided inside the centrifuge base (1).

4. A centrifuge for separating cells and media according to claim 3, wherein, The inner wall of the upper groove (5) is provided with connecting protrusions (51), which are spaced apart along the circumference of the upper groove (5).

5. A centrifuge for separating cells and media according to claim 4, wherein, The bottom of the upper cover (2) is provided with an electrical wire connector, and the centrifuge base (1) is provided with a waterproof connector seat (13) that is compatible with the electrical wire connector.

6. The centrifuge for separating cells and culture medium according to any one of claims 1 to 5, characterized in that, A horizontal groove (14) is provided on the outer wall of the centrifuge base (1). The first connecting frame (3) is damped and slidably disposed on the groove (14). The second connecting frame (4) is floatingly connected to the first connecting frame (3) through a telescopic rod (15). One end of the telescopic rod (15) is connected to the first connecting frame (3) and the other end is connected to the second connecting frame (4). A spring is provided inside the telescopic rod (15), and the telescopic rod (15) is extended by the spring.

Images