High-throughput sorting and storing device for urine stem cells

Abstract

The utility model relates to the technical field of storage devices, in particular to a urine stem cell high-flux sorting storage device which comprises a storage tank and a refrigeration box, the storage tank is used for sorting and storing urine stem cells, and the lower end of the storage tank is connected with the refrigeration box used for refrigeration and temperature control. According to the utility model, the small pipes in the peripheral area and the large pipes in the central area are formed through the partition layout of the large and small pipe storage sleeves and are combined with the linear connecting hole design of the sorting lifting plate, the batch sorting operation is supported, the high-flux requirement is met, and the two-way clamping design of the fixed clamping ring and the movable clamping ring (controlled by the positioning rod and the guide rod) is realized. The position stability of the freezing pipe in the sorting process is further improved, the refrigerating box conveys cold air to the center column through an air conveying pipe, the cold air is evenly diffused into the storage tank through air outlets of extension plates and branch plates, the interior of the storage tank is partitioned into small peripheral pipes and large center pipes through partition rings, and the space in the tank is utilized to the maximum extent; and the mixed storage requirements of freezing pipes of different specifications are met.

Description

Technical Field

[0001] This utility model relates to the field of storage device technology, and in particular to a high-throughput sorting and storage device for urine stem cells. Background Technology

[0002] Urinary stem cells are mesenchymal stem cells isolated from human or animal urine. These urine-derived stem cells are collected, processed, and stored long-term using a storage device. The stored stem cells can differentiate into nerve cells, cardiomyocytes, hepatocytes, etc., and can be used to treat diseases such as Parkinson's disease, cirrhosis, and spinal cord injury. Autologous stem cell transplantation can reduce the risk of immune rejection and improve treatment efficacy. The stored cells can also be used as raw materials for gene editing or customized treatments to match individualized medical needs.

[0003] Meanwhile, since the storage device uses a fixed storage rack to fix the freezing tubes, vibration occurs every time they are manually sorted, inserted, and removed. This vibration can cause the freezing tubes to loosen, tilt, or fall off, affecting the uniformity of low temperature. Furthermore, the process relies heavily on manual sorting, which is inefficient. Utility Model Content

[0004] To overcome the limitations of existing storage devices, which use fixed storage racks to hold cryotubes in place, the vibrations during manual sorting and insertion can cause the cryotubes to tilt or fall off, affecting the uniformity of low temperature. Furthermore, the reliance on manual sorting is inefficient. This invention provides a high-throughput sorting and storage device for urine stem cells.

[0005] The technical solution is as follows: A high-throughput sorting and storage device for urine stem cells includes a storage tank and a cooling box. The storage tank is used for sorting and storing urine stem cells. The lower end of the storage tank is connected to a cooling box for cooling and temperature control. A cover plate and a sorting lifting plate are provided on the top of the storage tank to protect the interior. The side of the storage tank is provided with a sorting lifting plate for lifting and lowering to place and retrieve urine stem cell cryopreservation tubes. Several sets of large-tube storage sleeves for high-throughput sorting are distributed circumferentially inside the storage tank. Several sets of small-tube storage sleeves for high-throughput sorting are distributed near the inner wall of the storage tank.

[0006] Furthermore, a central column is fixedly connected to the center of the storage tank, and a docking plate is connected to the top center of the central column. The side wall of the storage tank is provided with a sandwich layer, and a storage space is opened inside the sandwich layer. A partition ring is provided between the large tube storage sleeve and the small tube storage sleeve in the storage tank. The storage tank is divided into an outer area and a central area by the partition ring. The central column is connected to the refrigeration box, and there is a notch on the partition ring.

[0007] Furthermore, the interior of the central column is equipped with a cooling rod, and the outer end of the central column is provided with several sets of extension plates extending between the two sets of large tube storage sleeves. The upper side of the extension plates is fixed with branch plates, and both the branch plates and the extension plates are provided with several sets of air outlets for releasing cold air.

[0008] Furthermore, a first cooling plate is fixed to the bottom of the small tube storage sleeve, and several sets of first flow guide holes are distributed at the bottom of the first cooling plate. Several sets of first pressure spring rods are arranged circumferentially from bottom to top on the side wall of the small tube storage sleeve, and a spring damper is provided inside the first pressure spring rod.

[0009] Furthermore, a second cooling plate is fixedly connected to the bottom of the large tube storage sleeve. Several sets of second flow guide holes are distributed at the bottom of the second cooling plate. Several sets of second pressure spring rods are arranged circumferentially from bottom to top on the side wall of the large tube storage sleeve. A spring damper is provided inside the second pressure spring rod. An airbag head is fixedly connected to the end of the second pressure spring rod. A pressure sensor is fixedly connected to the top of the branch plate.

[0010] Furthermore, the top of the cover plate is close to several sets of temperature sensors, a rotating column is connected to the center of the cover plate, a sealing baffle is fitted on the rotating column, a drive motor is connected to the top of the rotating column, and an induction lamp is electrically connected to the top of the temperature sensor. Two sets of entry holes corresponding to the small tube storage sleeve and the large tube storage sleeve are linearly opened on the cover plate.

[0011] Furthermore, a push rod is connected to the bottom of the sorting lifting plate, and a fixed seat connected to the cover plate is sleeved at the lower end of the push rod. A first cylinder is provided at the bottom of the push rod. The first cylinder drives the push rod to move the sorting lifting plate up and down. The sorting lifting plate has linearly opened connection holes corresponding to the small tube storage sleeve and the large tube storage sleeve. A fixed retaining ring and a movable retaining ring are arranged opposite each other inside the connection hole. A positioning rod is provided at the center of the fixed retaining ring, and a guide rod is provided at the center of the movable retaining ring. A second cylinder is provided at one end of the guide rod. The second cylinder drives the guide rod to move the movable retaining ring towards the fixed retaining ring.

[0012] Furthermore, the bottom of the refrigeration box is provided with a refrigeration end, inside which is a controller and a refrigeration pump. The top of the refrigeration box is provided with gas supply pipes corresponding to the small tube storage sleeve and the large tube storage sleeve. Several sets of air exchange ports are circumferentially opened on the side wall of the refrigeration box.

[0013] The beneficial effects are as follows: This utility model realizes automatic positioning and gripping of frozen tubes through the pneumatic drive of the sorting lifting plate and the linkage of the push rod and the retaining ring, reducing manual intervention. The partitioned layout of the large and small tube storage sleeves forms a small tube in the outer area and a large tube in the central area. Combined with the linear connection hole design of the sorting lifting plate, it supports batch sorting operations and adapts to high throughput requirements. The bidirectional clamping design of the fixed retaining ring and the movable retaining ring (controlled by the positioning rod and the guide rod) further improves the positional stability of the frozen tubes during the sorting process. The refrigeration box delivers cold air to the central column through the air supply pipe. The cold air is evenly diffused into the interior of the storage tank through the air outlet of the extension plate and the branch plate. The storage tank is divided into outer small tubes and central large tubes by the partition ring, maximizing the use of the internal space and adapting to the mixed storage requirements of frozen tubes of different specifications. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of a high-throughput sorting and storage device for urine stem cells according to the present invention.

[0015] Figure 2 This is a schematic diagram of the internal structure of the storage tank of this utility model;

[0016] Figure 3 This is a three-dimensional structural diagram of the small tube storage sleeve of this utility model;

[0017] Figure 4 This is a three-dimensional structural diagram of the large-tube storage sleeve of this utility model;

[0018] Figure 5 This is an exploded three-dimensional structural diagram of the small tube storage sleeve, the large tube storage sleeve, and the central column of this utility model;

[0019] Figure 6 This is a three-dimensional structural diagram of the cover plate of this utility model;

[0020] Figure 7 This is a three-dimensional structural diagram of the sorting and lifting plate of this utility model;

[0021] Figure 8 This is a three-dimensional structural diagram of the refrigeration box of this utility model.

[0022] In the attached diagram, the following are the reference numerals: 1. Storage tank; 2. Cover plate; 3. Sorting and lifting plate; 4. Refrigeration box; 101. Interlayer; 102. Storage space; 103. Partition ring; 104. Outer area; 105. Central area; 106. Central column; 107. Connecting plate; 108. Small tube storage sleeve; 109. Large tube storage sleeve; 110. First contact spring rod; 111. First cooling plate; 112. First guide hole; 113. Second contact spring rod; 114. Airbag head; 115. Second cooling plate; 116. Second guide hole; 17. Extension plate; 118. Airflow sensor; 119. Branch plate; 118. Air pressure sensor; 120. Air outlet; 121. Notch; 201. Temperature sensor; 202. Sensor light; 203. Drive motor; 204. Sealing baffle; 205. Inlet hole; 301. Push rod; 302. Fixing base; 303. First cylinder; 304. Connection hole; 305. Fixing retaining ring; 306. Movable retaining ring; 307. Second cylinder; 308. Positioning rod; 401. Air exchange port; 402. Refrigeration end; 403. Refrigeration pump. Detailed Implementation

[0023] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0024] Urine contains renal tubular epithelial cells, bladder mucosal cells, and other biological samples that can be transformed into induced pluripotent stem cells (iPS cells) through reprogramming technology. Compared with traditional stem cell sources (such as umbilical cord blood and bone marrow), urine collection has core advantages such as non-invasiveness, reproducibility, and lack of ethical controversy. For example, the Zhenxi Valley team used EMT-MET cell fate regulation technology to reverse the renal exfoliated epithelial cells in urine into iPS cells with multi-directional differentiation potential, solving the problems of invasiveness and ethical restrictions in traditional stem cell collection.

[0025] Clinical application prospects of urine stem cells

[0026] Urine-derived iPS cells can differentiate into functional cells such as nerve cells, bone cells, and liver cells, and show potential in fields such as anti-aging, tissue repair, and cancer treatment. For example, in the treatment of chronic kidney disease, stem cells can repair renal parenchymal cells through immune regulation and nutritional support, thus slowing the progression of the disease.

[0027] Urine samples require multiple steps, including pretreatment, reprogramming, and amplification, to be prepared into iPS cells, resulting in large batch sizes and high storage densities. Existing equipment struggles to achieve rapid access and dynamic management of high-throughput samples, easily leading to sample confusion or cryopreservation failure.

[0028] Human urinary stem cells (hUSCs) can repair bladder mucosal damage and relieve urinary frequency, urgency, and pelvic pain through autologous transplantation. hUSCs have high biocompatibility with bladder tissue, can secrete anti-inflammatory factors (such as IL-10) and inhibit fibrosis, reducing the risk of immune rejection. Animal experiments have shown that injecting hUSCs into the bladder wall can reduce inflammatory cell infiltration and promote bladder smooth muscle regeneration.

[0029] Urine stem cell storage

[0030] Urine stem cell storage refers to the process of collecting human urine samples non-invasively and isolating stem cells with multi-directional differentiation potential from them.

[0031] Technical process: Urine collection → Stem cell isolation and reprogramming → Expansion culture → Liquid nitrogen cryopreservation

[0032] Key features: non-invasive, no ethical controversy, reproducible, and the stored stem cells can maintain their activity for a long time;

[0033] It is used to repair chronic kidney disease, interstitial cystitis, etc. It can promote tissue regeneration by differentiating into renal tubular epithelial cells or secreting anti-inflammatory factors. iPS cells can be directed to differentiate into nerve cells for cell replacement therapy in Parkinson's disease and Alzheimer's disease. It can also differentiate into cardiomyocytes or vascular endothelial cells to repair myocardial infarction or arteriosclerosis damage.

[0034] Intervention for urinary system diseases

[0035] Urinary stem cells can differentiate into renal tubular epithelial cells, bladder mucosal cells, etc., and can be used to repair urinary system diseases such as chronic kidney disease and interstitial cystitis. They secrete anti-inflammatory factors (such as IL-10) and growth factors through paracrine function, promoting the regeneration of damaged tissue and inhibiting fibrosis.

[0036] Case Study: In a mouse model of acute kidney injury, urinary stem cell transplantation significantly reduced plasma urea nitrogen levels and accelerated glomerular structural repair.

[0037] Treatment of neurological diseases

[0038] Urinary induced pluripotent stem cells (iPS cells) can differentiate into nerve cells and are used for cell replacement therapy in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Nerve growth factors (such as BDNF) secreted by stem cells can activate the regeneration of damaged neurons.

[0039] Cardiovascular repair and metabolic regulation

[0040] Urinary stem cells differentiated into cardiomyocytes or vascular endothelial cells can repair heart tissue after myocardial infarction. The cytokines they secrete (such as VEGF) can promote angiogenesis and improve blood supply to ischemic sites. At the same time, stem cells can delay the progression of metabolic diseases such as diabetes by regulating pancreatic function and glucose and lipid metabolism.

[0041] Skin regeneration and anti-wrinkle

[0042] Urinary stem cells secrete exosomes rich in growth factors such as EGF and FGF, which can stimulate the proliferation of skin fibroblasts, promote collagen synthesis, and reduce UV damage and wrinkle formation.

[0043] Strengthening immune function

[0044] Stem cells can reduce chronic inflammatory responses and delay organ degeneration (such as arthritis and arteriosclerosis) by inhibiting overactivated T lymphocytes and regulating macrophage polarization.

[0045] Brain function rejuvenation

[0046] After stem cells are reinfused, they differentiate into new nerve cells, replenishing the brain neurons that are reduced due to aging (0.8% reduction per year after age 25), improving memory, cognitive decline, and symptoms of Alzheimer's disease. Urinary stem cells are derived from the patient's own body, with low MHC-II molecule expression, and the risk of rejection from allogeneic transplantation is significantly lower than that of bone marrow or umbilical cord blood stem cells. Stored stem cells can be used as raw materials for gene editing and 3D bioprinted organs for customized tissue repair or organ transplantation.

[0047] Viability assurance: The cryopreservation solution formulation needs to be optimized to reduce freeze-thaw damage (current revival survival rate is about 70%).

[0048] Standardization system: Establish quality control standards for the entire process from collection to storage (such as cell viability testing and contamination control);

[0049] Clinical translation: Accelerating clinical trials for diseases such as uremia and Parkinson's disease, promoting the application of technology, and urine stem cell storage has become a cutting-edge technology in the field of regenerative medicine through its triple value of disease intervention, anti-aging and personalized medicine. Its non-invasiveness and high adaptability provide a core resource reserve for future precision medicine. It can be kept active for a long time in a liquid nitrogen environment at -196℃ and can be used at any time to deal with sudden diseases or health problems caused by aging, which is equivalent to "bioinsurance".

[0050] like Figures 1-4As shown, a high-throughput sorting and storage device for urine stem cells includes a storage tank 1 and a cooling box 4. The storage tank 1 is used for sorting and storing urine stem cells. The lower end of the storage tank 1 is connected to the cooling box 4 for cooling and temperature control. There is a cover plate 2 and a sorting lifting plate 3. The top of the storage tank 1 is fitted with a cover plate 2 for protecting the interior. The side of the storage tank 1 is provided with a sorting lifting plate 3 for lifting and lowering to place and take out urine stem cell cryopreservation tubes. Several sets of large tube storage sleeves 109 for high-throughput sorting are distributed circumferentially inside the storage tank 1. Several sets of small tube storage sleeves 108 for high-throughput sorting are distributed near the inner wall of the storage tank 1.

[0051] Please see Figures 2-3 In this embodiment, a central column 106 is fixedly connected to the center of the storage tank 1. A docking plate 107 is connected to the center of the top of the central column 106. A sandwich layer 101 is provided on the side wall of the storage tank 1. A storage space 102 is provided inside the sandwich layer 101. A partition ring 103 is provided between the large tube storage sleeve 109 and the small tube storage sleeve 108 in the storage tank 1. The storage tank 1 is divided into an outer area 104 and a central area 105 by the partition ring 103. The central column 106 is connected to the refrigeration box 4. A notch 121 is provided on the partition ring 103. A refrigeration rod is provided inside the central column 106. Several sets of extension plates 117 extending to the two sets of large tube storage sleeves 109 are provided circumferentially at the outer end of the central column 106. A branch plate 119 is fixedly connected to the upper side of the extension plate 117. Several sets of air outlets 120 for releasing cold air are provided on both the branch plate 119 and the extension plate 117.

[0052] Please see Figures 2-3 In this embodiment, a first cooling plate 111 is fixedly connected to the bottom of the small tube storage sleeve 108. Several sets of first flow guide holes 112 are distributed at the bottom of the first cooling plate 111. Several sets of first pressure spring rods 110 are arranged circumferentially from bottom to top on the side wall of the small tube storage sleeve 108. A spring damper is provided inside the first pressure spring rod 110. A second cooling plate 115 is fixedly connected to the bottom of the large tube storage sleeve 109. Several sets of second flow guide holes 116 are distributed at the bottom of the second cooling plate 115. Several sets of second pressure spring rods 113 are arranged circumferentially from bottom to top on the side wall of the large tube storage sleeve 109. A spring damper is provided inside the second pressure spring rod 113. An airbag head 114 is fixedly connected to the end of the second pressure spring rod 113 extending into the large tube storage sleeve 109. A pressure sensor 118 is fixedly connected to the top of the branch plate 119.

[0053] Please see Figures 2-3In this embodiment, the top of the cover plate 2 is close to several sets of temperature sensors 201. A rotating column is connected to the center of the cover plate 2, and a sealing baffle 204 is fitted on the rotating column. A drive motor 203 is connected to the top of the rotating column. A sensor lamp 202 is electrically connected to the top of the temperature sensor 201. Two sets of entry holes 205 corresponding to small tube storage sleeves 108 and large tube storage sleeves 109 are linearly opened on the cover plate 2. A push rod 301 is connected to the bottom of the sorting lifting plate 3. A fixing seat 302 connected to the cover plate 2 is fitted at the lower end of the push rod 301. A first cylinder 303 is provided at the bottom of the push rod 301. The first cylinder 303 drives the push rod 301 to drive the sorting lifting plate 3 to rise and fall. Corresponding small tube storage sleeves 108 and 209 are linearly opened on the sorting lifting plate 3. The connection hole 304 between 08 and the large tube storage sleeve 109 has a fixed retaining ring 305 and a movable retaining ring 306 arranged opposite each other inside the connection hole 304. The fixed retaining ring 305 has a positioning rod 308 at its center, and the movable retaining ring 306 has a guide rod at its center. One end of the guide rod has a second cylinder 307. The second cylinder 307 drives the guide rod to move the movable retaining ring 306 toward the fixed retaining ring 305. The bottom of the refrigeration box 4 has a refrigeration end 402. The refrigeration end 402 has a controller and a refrigeration pump 403 inside. The top of the refrigeration box 4 has air supply pipes corresponding to the small tube storage sleeve 108 and the large tube storage sleeve 109. The side wall of the refrigeration box 4 has several sets of air exchange ports 401 circumferentially opened.

[0054] During storage, large and small freezer tubes are inserted into the connection hole 304. The second cylinder 307 drives the guide rod to move the movable retaining ring 306 towards the fixed retaining ring 305. The retaining ring clamps and precisely grasps the target freezer tube. The first cylinder 303 drives the push rod 301 to raise and lower the sorting lifting plate 3 to the appropriate position. The drive motor 203 drives the rotating column to rotate the sealing baffle 204. The freezer tube is inserted into the corresponding large and small tube storage sleeve 108 through the inlet hole 205 of the cover plate 2. The contact spring rod clamps the tube body through elastic damping force. The airbag head 114 (large tube) further buffers external vibration. After the refrigeration box 4 is started, cold air enters the control of the central column 106 through the gas supply pipe. The cold air is evenly released through the air outlet 120 of the extension plate 117 and the branch plate 119. The cold air circulates along the guide holes of the cold guide plate to form a three-dimensional cold coverage, ensuring uniform temperature inside the tank. When the push rod 301 drives the sorting lifting plate 3 to rise, the freezing tube is separated from the storage sleeve along with the sorting lifting plate 3. The spring rod is automatically reset to avoid interfering with adjacent samples. The cold air continuously maintains a low temperature environment through the circulation path formed by the central column 106 and the notch 121 of the partition ring 103, avoiding local overheating. The temperature sensor 201 feeds the data back to the controller to dynamically adjust the power of the refrigeration pump 403. The double-layer structure of the sealing baffle 204 and the cover plate 2 isolates external moisture and ensures the airtightness of the storage environment.

[0055] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A high-throughput sorting and storage device for urine stem cells, comprising a storage tank (1) and a cooling box (4), wherein the storage tank (1) is used for sorting and storing urine stem cells, and the lower end of the storage tank (1) is connected to the cooling box (4) for cooling and temperature control, characterized in that; It also includes a cover plate (2) and a sorting lifting plate (3). The top of the storage tank (1) is fitted with a cover plate (2) for protecting the interior. The side of the storage tank (1) is provided with a sorting lifting plate (3) for lifting and lowering to place and retrieve urine stem cell cryopreservation tubes. Several sets of large-tube storage sleeves (109) for high-throughput sorting are distributed circumferentially inside the storage tank (1). Several sets of small-tube storage sleeves (108) for high-throughput sorting are distributed near the inner wall inside the storage tank (1).

2. The urine stem cell high throughput sorting storage device of claim 1, wherein, A central column (106) is fixedly connected to the center inside the storage tank (1). A docking plate (107) is connected to the center of the top of the central column (106). A sandwich layer (101) is provided on the side wall of the storage tank (1). A storage space (102) is provided inside the sandwich layer (101). A partition ring (103) is provided between the large tube storage sleeve (109) and the small tube storage sleeve (108) in the storage tank (1). The storage tank (1) is divided into an outer area (104) and a central area (105) by the partition ring (103). The central column (106) is connected to the refrigeration box (4). The partition ring (103) has a notch (121).

3. The urine stem cell high throughput sorting storage device of claim 2, wherein, The center column (106) is equipped with a cooling rod inside. Several sets of extension plates (117) extending to the two sets of large tube storage sleeves (109) are provided around the outer end of the center column (106). A branch plate (119) is fixed to the upper end of the extension plate (117). Several sets of air outlets (120) for releasing cold air are opened on both the branch plate (119) and the extension plate (117).

4. The urine stem cell high throughput sorting storage device of claim 1, wherein, The bottom of the small tube storage sleeve (108) is fixedly connected to a first cooling plate (111). Several sets of first flow guide holes (112) are distributed at the bottom of the first cooling plate (111). Several sets of first pressure spring rods (110) are arranged in the circumferential direction from bottom to top on the side wall of the small tube storage sleeve (108). A spring damper is provided inside the first pressure spring rod (110).

5. The urine stem cell high throughput sorting storage device of claim 1, wherein, A second cooling plate (115) is fixedly connected to the bottom of the large tube storage sleeve (109). Several sets of second flow guide holes (116) are distributed at the bottom of the second cooling plate (115). Several sets of second pressure spring rods (113) are arranged circumferentially from bottom to top on the side wall of the large tube storage sleeve (109). A spring damper is provided inside the second pressure spring rod (113). An airbag head (114) is fixedly connected to the end of the second pressure spring rod (113) that penetrates into the large tube storage sleeve (109). A pressure sensor (118) is fixedly connected to the top of the branch plate (119).

6. The urine stem cell high throughput sorting storage device of claim 1, wherein, The top of the cover plate (2) is close to several sets of temperature sensors (201). A rotating column is connected to the center of the cover plate (2). A sealing baffle (204) is fitted on the rotating column. A drive motor (203) is connected to the top of the rotating column. An induction lamp (202) is electrically connected to the top of the temperature sensor (201). Two sets of entry holes (205) corresponding to the small tube storage sleeve (108) and the large tube storage sleeve (109) are linearly opened on the cover plate (2).

7. The urine stem cell high throughput sorting storage device of claim 1, wherein, A push rod (301) is connected to the bottom of the sorting lifting plate (3). A fixed seat (302) connected to the cover plate (2) is sleeved at the lower end of the push rod (301). A first cylinder (303) is provided at the bottom of the push rod (301). The first cylinder (303) drives the push rod (301) to drive the sorting lifting plate (3) to rise and fall. A connecting hole (304) corresponding to the small tube storage sleeve (108) and the large tube storage sleeve (109) is linearly opened on the sorting lifting plate (3). A fixed retaining ring (305) and a movable retaining ring (306) are arranged opposite to each other inside the connecting hole (304). A positioning rod (308) is provided at the center of the fixed retaining ring (305). A guide rod is provided at the center of the movable retaining ring (306). A second cylinder (307) is provided at one end of the guide rod. The second cylinder (307) drives the guide rod to move the movable retaining ring (306) towards the fixed retaining ring (305).

8. The urine stem cell high throughput sorting storage device of claim 1, wherein, The bottom of the refrigeration box (4) is provided with a refrigeration end (402). The refrigeration end (402) is provided with a controller and a refrigeration pump (403) inside. The top of the refrigeration box (4) is provided with gas supply pipes corresponding to the small tube storage sleeve (108) and the large tube storage sleeve (109). The side wall of the refrigeration box (4) is provided with several sets of air exchange ports (401) in a circumferential manner.

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