Sterile disinfection air-drying device for separating endophytic fungi of grass stems and seeds

Abstract

The utility model belongs to the field of sterile disinfection and air drying for separating endophytic fungi of grass stems and seeds, particularly relates to a sterile disinfection and air drying device for separating endophytic fungi of grass stems and seeds, and aims to solve the problems that in the existing separation culture process, grass stems and seeds are disinfected by disinfectant fluid (alcohol or sodium hypochlorite) manually by experimenters; in order to solve the problems that the probability of pollution is easily increased, the steps are dispersed, time and labor are consumed and the efficiency is low in the process of cutting the stems and the seeds into small sections, the following scheme is provided: the device comprises a box body, supporting legs for supporting are fixedly arranged on the two sides of the bottom of the box body, and the supporting legs are fixedly arranged on the two sides of the bottom of the box body; a placing groove is formed in one side of the top of the box body, grass stalks and seeds can be automatically treated through the clamping mechanism, the blow-drying mechanism, the cutting mechanism, the moving mechanism and a second electric push rod, the efficiency is higher, simplicity and convenience are achieved, and the possibility of pollution in the bacterium separation and callus induction process is effectively reduced.

Description

Technical Field

[0001] This utility model relates to the field of aseptic disinfection and air drying technology for separating endophytic fungi from grass stems and seeds, and particularly to an aseptic disinfection and air drying device for separating endophytic fungi from grass stems and seeds. Background Technology

[0002] Endophytic fungal strains of grasses are isolated, purified, and cultured from grass seeds or plants grown from seeds.

[0003] The existing isolation and culture process has the following problems:

[0004] In existing isolation and culture processes, grass stems and seeds are disinfected manually by laboratory personnel using disinfectant (alcohol or sodium hypochlorite), and then the moisture in the grass stems and seeds is absorbed with filter paper. The stems are then cut into small sections on the filter paper. However, this process easily increases the probability of contamination, and the steps are scattered, time-consuming, labor-intensive, and inefficient. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies, such as the manual disinfection of grass stems and seeds by laboratory personnel using disinfectant (alcohol or sodium hypochlorite), followed by absorbing the moisture with filter paper and then cutting the stems into small sections on the filter paper. However, this process is prone to contamination, and the steps are scattered, time-consuming, labor-intensive, and inefficient. The invention proposes a sterile disinfection and drying device for isolating endophytic fungi from grass stems and seeds.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A sterile disinfection and air-drying device for isolating endophytic fungi from grass stems and seeds includes a box body. Support legs are fixedly installed on both sides of the bottom of the box body. A placement groove is opened on one side of the top of the box body, and a disinfection box containing disinfectant and a treatment box for draining grass stems and seeds after disinfection are placed in the placement groove. A transparent door is installed on one side of the box body by a hinge.

[0008] A clamping mechanism is installed inside the box to clamp the straw stalks;

[0009] The drying mechanism is installed on the box and is used to dry the straw stalks and seeds after sterile water treatment;

[0010] The cutting mechanism is installed inside the box and is used to cut the dried straw stalks.

[0011] The moving mechanism, located inside the box, is used to move the clamped straw stalks to the corresponding positions of the drying and cutting mechanisms.

[0012] In one possible design, the clamping mechanism includes a U-shaped frame, a first electric push rod, a first clamping plate, and a second clamping plate. One end of the first electric push rod is fixedly connected to one side of the frame, and the telescopic part passes through one side of the frame and is fixedly connected to one side of the first clamping plate. One side of the second clamping plate is fixedly connected to one side of the inner wall of the frame.

[0013] In one possible design, the drying mechanism includes a blower box and a fan. A through hole is opened on one side of the box body, and the outer side of the blower box is fixedly connected to the inner wall of the through hole. Multiple air holes are opened on one side of the blower box. The air outlet of the fan is fixedly connected to and communicates with one side of the blower box. An exhaust port is opened on one side of the box body, and a filter screen is fixed to one side of the box body by screws. The filter screen covers the exhaust port. The blower box is located above the processing box.

[0014] In one possible design, the cutting mechanism includes a short plate, a bidirectional lead screw, two movable plates, and blades. One side of the short plate is fixedly connected to the inner wall of one side of the housing. One end of the bidirectional lead screw is rotatably connected to one side of the short plate. A second motor for driving the bidirectional lead screw to rotate is fixedly installed on one side of the housing, and the output end of the second motor passes through one side of the housing and is fixedly connected to the other end of the bidirectional lead screw. A slider is fixedly installed on one side of each of the two movable plates. A groove is formed on the inner wall of one side of the housing, and one side of the slider is slidably connected to the inner wall of the groove. A first lead screw nut is embedded in one side of each movable plate and is threadedly connected to the bidirectional lead screw. A blade plate and a blade holder are fixedly installed on the sides of the two movable plates that are close to each other. The blade is fixed to the blade holder by bolts. A collection box for collecting the cut grass stalks is placed on the inner wall of the bottom of the housing.

[0015] In one possible design, the moving mechanism includes a U-shaped frame, a lead screw, a moving block, and a first motor for driving the lead screw to move. The top of the U-shaped frame is fixedly connected to the inner wall of the top of the housing. One end of the lead screw is rotatably connected to the inner wall of one side of the housing. One side of the first motor is fixedly connected to one side of the housing, and its output end passes through one side of the housing and is fixedly connected to the other end of the lead screw. The top of the moving block is slidably connected to the inner wall of the top of the U-shaped frame. A second lead screw nut is embedded in one side of the moving block, and the second lead screw nut is threadedly connected to the lead screw.

[0016] In one possible design, a second electric push rod for adjusting the height of the grass stalks is fixedly installed at the bottom of the movable block, and the telescopic part of the second electric push rod is fixedly connected to the top of the frame.

[0017] In one possible design, a baffle made of transparent material is fixedly installed on one side of the top of the processing box to prevent liquid on the straw stems from blowing onto the transparent door.

[0018] In one possible design, the bottom inner wall of the sterilization box is fixedly provided with multiple partitions, and the sterilization box is divided into six areas by the partitions. A mesh bag for placing seeds is detachably fixed to one side of the first clamp.

[0019] In this application, during use, the entire unit is placed inside a clean bench. The transparent door is then opened, and an appropriate amount of disinfectant is injected into the disinfection box (since the disinfection box is divided into six areas by partitions, the six areas from left to right are alcohol, distilled water, sodium hypochlorite, and three sterile water solutions). The collection box is placed inside the box and below the cutting mechanism. Then, the straw stalks are vertically placed between the first and second clamping plates. The first electric push rod is activated, and the telescopic part pushes the first clamping plate towards the second clamping plate, thereby clamping and fixing the top of the straw stalks. The clamping mechanism is initially positioned above the disinfection box, and can then be closed. The transparent door opens, and the second electric push rod is activated. The telescopic part of the second electric push rod extends and retracts, immersing the stem in the disinfection tank for disinfection. During disinfection, the second electric push rod can be extended and retracted to move the stem up and down in the disinfectant water, improving the disinfection effect. Alternatively, the first motor can drive the stem to move in the disinfectant water. After disinfection, the second electric push rod lifts the stem, and the first motor is activated. The first motor drives the lead screw to rotate, and the moving block moves laterally along the U-shaped frame via the second lead screw nut, moving the stem to the sterile water position. Then, the second electric push rod immerses the bottom end of the stem in the sterile water for further treatment. The second electric push rod retracts, removing the stem from the sterile water and moving it above the processing chamber. The blower is then activated, and airflow is evenly directed onto the stem through the blower holes, drying it. Airflow inside the chamber is discharged through the outlet. A baffle prevents liquid from splashing onto the transparent door. After drying, the first motor moves the stem between the two moving plates of the cutting mechanism. The stem height is adjusted according to the cutting dimensions (this can be done via the second electric push rod). Once the height is adjusted, the second motor drives the bidirectional lead screw, causing the two moving plates to move towards each other, engaging the blade and the cutting plate. The stem is cut, and the cut stem segments fall freely into the collection box. The transparent door can then be opened for removal. The plant stems can be processed automatically through a clamping mechanism, a drying mechanism, a cutting mechanism, a moving mechanism, and a second electric push rod, which is more efficient, simple, and convenient. It effectively reduces the possibility of contamination during the induction of mycelium and callus. Seeds can be placed in the net bag for disinfection. The mesh size of the net bag is smaller than that of the seeds. The net bag is detachable (it can be fixed with bolts, buckles, etc.) and is set on the first clamping plate for easy removal of the seeds.

[0020] The first motor, second motor, first electric push rod, second electric push rod, and fan require an external power supply and an external controller for operation. The first and second motors can be stepper motors, which can rotate in both directions. The transparent door and baffle can be made of transparent acrylic or tempered glass, while the lead screw and bidirectional lead screw can be made of stainless steel. The specific materials can be selected according to the needs. It should be noted that multiple components such as the disinfection box, blades, and processing box need to be cleaned and disinfected regularly (after each use).

[0021] In this utility model, the sterile disinfection and drying device for separating endophytic fungi from grass stems and seeds uses a clamping mechanism to vertically hold the grass stems between the first clamping plate and the second clamping plate. When the first electric push rod is activated, the telescopic part pushes the first clamping plate to the second clamping plate, thereby clamping and fixing the top of the grass stems.

[0022] In this invention, the sterile disinfection and drying device for separating endophytic fungi from grass stems and seeds uses a drying mechanism to start a fan, and the airflow is evenly blown onto the stems through the air holes of the air box, thereby drying the stems or seeds.

[0023] In this invention, the sterile disinfection and drying device for separating endophytic fungi from grass stems and seeds uses a cutting mechanism to start a second motor to drive a bidirectional lead screw, causing two moving plates to move in opposite directions. The blade and the cutting plate work together to complete the cutting, thereby cutting the stems.

[0024] In this invention, the sterile disinfection and drying device for separating endophytic fungi from grass stems and seeds uses a moving mechanism to start a first motor, which drives a lead screw to rotate. The moving block moves laterally along the U-shaped frame via a second lead screw nut, moving the stems to the corresponding positions of the drying mechanism and the cutting mechanism.

[0025] In this invention, the clamping mechanism, drying mechanism, cutting mechanism, moving mechanism, and second electric push rod can automate the processing of grass stems and seeds, resulting in higher efficiency, simplicity, and convenience, and effectively reducing the possibility of contamination during the induction of bacteria and callus tissue. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the internal structure of the sterile disinfection and drying device for isolating endophytic fungi from grass stems and seeds proposed in this utility model.

[0027] Figure 2 This is a schematic diagram of the main structure of the sterile disinfection and drying device for separating endophytic fungi from grass stems and seeds proposed in this utility model.

[0028] Figure 3This is a side view of the sterile disinfection and drying device for isolating endophytic fungi from grass stems and seeds proposed in this utility model.

[0029] Figure 4 This is a side view of the internal structure of the sterile disinfection and drying device for isolating endophytic fungi from grass stems and seeds proposed in this utility model.

[0030] Figure 5 This is a side sectional view of the sterile disinfection and drying device for isolating endophytic fungi from grass stems and seeds, as proposed in this utility model.

[0031] In the diagram: 1. Box body; 2. Transparent door; 3. First motor; 4. Air blower; 5. Fan; 6. Second motor; 7. Filter screen; 8. Baffle; 9. Disinfection box; 10. Processing box; 11. Short board; 12. Moving plate; 13. Two-way lead screw; 14. U-shaped frame; 15. First clamping plate; 16. First electric push rod; 17. Frame body; 18. Second electric push rod; 19. Moving block; 20. Blade; 21. Collection box. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0033] Example 1

[0034] Reference Figure 1-5 The aseptic disinfection and drying device includes: a cabinet 1 with fixed feet on both sides of its bottom to ensure stability. A placement slot is provided on one side of the top of the cabinet 1, containing a disinfection box 9 and a processing box 10. The disinfection box 9 contains disinfectant and is divided into six areas by partitions. A transparent door 2, made of transparent acrylic or tempered glass, is hinged to the front of the cabinet 1 for easy observation of internal operations and cleaning and disinfection. The transparent door 2 is equipped with a lock.

[0035] The clamping mechanism consists of a U-shaped frame 17, a first electric push rod 16, a first clamping plate 15, and a second clamping plate. The frame 17 is fixed to the bottom of the movable block 19. The first electric push rod 16 is horizontally installed on one side of the frame 17, and its telescopic end drives the first clamping plate 15 to close towards the second clamping plate, achieving flexible clamping of the stem tip. The second clamping plate is fixed to the inner wall of the frame 17, and its surface is covered with a silicone anti-slip pad to avoid damaging plant tissue. A net bag for placing seeds is detachably fixed to one side of the first clamping plate 15.

[0036] The drying mechanism consists of an air box 4 and a fan 5. The air box 4 is embedded in the through-hole on the side wall of the housing 1, and its air outlet has multiple evenly distributed circular air holes with a diameter of 2mm to ensure that the airflow evenly covers the surface of the stem. The fan 5 is a centrifugal fan, which is connected to the air box 4 through the air outlet of the fan 5, and the rated air volume is adjustable. A HEPA high-efficiency filter 7 with a filtration efficiency of ≥99.99% is installed at the outlet of the housing 1.

[0037] The cutting mechanism includes a short plate 11, a bidirectional lead screw 13, a moving plate 12, a blade plate, a blade holder, and a blade 20. The short plate 11 is fixed to the inner wall of the housing 1, and one end of the bidirectional lead screw 13 is rotatably connected to it, while the other end is driven by a second motor 6. The two moving plates 12 achieve linear motion by engaging with the sliding groove of the housing 1 via sliders, and the blade plate and blade holder are respectively installed on their inner sides. The blade 20 adopts a detachable design and is fixed to the blade holder with bolts for easy replacement and maintenance. During cutting, the second motor 6 drives the bidirectional lead screw 13 to rotate, causing the moving plates 12 to move towards each other to complete the cutting. The cut stem segments fall into the bottom collection box 21. The moving plate 12 is made of 304 stainless steel, the blade plate surface is electrolytically polished, and the blade 20 is made of medical-grade stainless steel with a cutting edge angle of 15°.

[0038] The moving mechanism consists of a U-shaped frame 14, a lead screw, and a first motor 3. The lead screw is arranged along the width of the housing, and the first motor 3 drives its rotation. The moving block 19 engages with the lead screw via a second lead screw nut, enabling lateral movement on the guide rail of the U-shaped frame 14.

[0039] This application is used in the field of aseptic disinfection and air drying for the isolation of endophytic fungi from grass stems and seeds, and can also be used in other fields applicable to this application.

[0040] Example 2

[0041] refer to Figure 1-5 An improvement based on Example 1: a sterile disinfection and air-drying device for separating endophytic fungi from grass stems and seeds, which is applied in the field of sterile disinfection and air-drying for separating endophytic fungi from grass stems and seeds.

[0042] A second electric push rod 18 is installed at the bottom of the movable block 19, and its telescopic end is connected to the clamping mechanism to realize the vertical position adjustment of the stem. This adapts to the processing needs of samples of different lengths.

[0043] However, as is well known to those skilled in the art, the working principles and wiring methods of the first motor 3, the second motor 6, the first electric push rod 16, the second electric push rod 18 and the fan 5 are commonplace and belong to conventional methods or common knowledge. They will not be described in detail here. Those skilled in the art can make any selections according to their needs or convenience.

[0044] The accompanying drawings in this application are for illustrative purposes only. The dimensions and shapes of the components shown are not actual limitations but are merely schematic representations. In actual implementation, the components can be reasonably configured and adjusted according to specific needs and actual conditions.

[0045] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A sterile, disinfected, air-drying apparatus for isolating endophytic fungi from the stems and seeds of grasses, comprising a box (1), characterized in that, Both sides of the bottom of the box (1) are fixed with support legs. A placement groove is opened on one side of the top of the box (1), and a disinfection box (9) containing disinfectant water and a treatment box (10) for draining after disinfection of grass stems or seeds are placed in the placement groove. A transparent door (2) is installed on one side of the box (1) by a hinge. The clamping mechanism is installed inside the box (1) to clamp the straw stalks; The drying mechanism is installed on the box (1) and is used to dry the straw stalks or seeds after sterile water treatment; The cutting mechanism is installed inside the box (1) and is used to cut the dried straw stalks; The moving mechanism is set inside the box (1) to move the clamped straw stalks to the corresponding positions of the drying mechanism and the cutting mechanism.

2. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation according to claim 1, characterized by, The clamping mechanism includes a U-shaped frame (17), a first electric push rod (16), a first clamping plate (15), and a second clamping plate. One end of the first electric push rod (16) is fixedly connected to one side of the frame (17), and the telescopic part passes through one side of the frame (17) and is fixedly connected to one side of the first clamping plate (15). One side of the second clamping plate is fixedly connected to one side of the inner wall of the frame (17).

3. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation according to claim 1, characterized by, The drying mechanism includes a blower box (4) and a fan (5). A through hole is opened on one side of the box body (1). The outer side of the blower box (4) is fixedly connected to the inner wall of the through hole. Multiple air holes are opened on one side of the blower box (4). The air outlet of the fan (5) is fixedly connected to and communicates with one side of the blower box (4). An outlet is opened on one side of the box body (1). A filter screen (7) is fixed on one side of the box body (1) by screws. The filter screen (7) covers the outlet. The blower box (4) is located above the processing box (10).

4. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation of claim 1, wherein, The cutting mechanism includes a short plate (11), a bidirectional lead screw (13), two moving plates (12), and a blade (20). One side of the short plate (11) is fixedly connected to the inner wall of one side of the housing (1). One end of the bidirectional lead screw (13) is rotatably connected to one side of the short plate (11). A second motor (6) for driving the bidirectional lead screw (13) to rotate is fixedly installed on one side of the housing (1), and the output end of the second motor (6) passes through one side of the housing (1) and is fixedly connected to the other end of the bidirectional lead screw (13). A slider is fixedly installed on one side of each movable plate (12). A groove is opened on the inner wall of one side of the box (1). One side of the slider is slidably connected to the inner wall of the groove. A first screw nut is embedded in one side of the movable plate (12), and the first screw nut is threadedly connected to the double screw (13). A blade plate and a blade holder are fixedly installed on the side of the two movable plates (12) that are close to each other. The blade (20) is fixed to the blade holder by bolts. A collection box (21) for collecting the cut grass stalks is placed on the bottom inner wall of the box (1).

5. The aseptic sterilization and drying device for isolating endophytic fungi from grass stems and seeds according to claim 1, characterized in that, The moving mechanism includes a U-shaped frame (14), a lead screw, a moving block (19), and a first motor (3) for driving the lead screw to move. The top of the U-shaped frame (14) is fixedly connected to the top inner wall of the housing (1). One end of the lead screw is rotatably connected to the inner wall of one side of the housing (1). One side of the first motor (3) is fixedly connected to one side of the housing (1), and its output end passes through one side of the housing (1) and is fixedly connected to the other end of the lead screw. The top of the moving block (19) is slidably connected to the top inner wall of the U-shaped frame (14). A second lead screw nut is embedded in one side of the moving block (19), and the second lead screw nut is threadedly connected to the lead screw.

6. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation according to claim 5, characterized by, The bottom of the movable block (19) is fixedly provided with a second electric push rod (18) for adjusting the height of the grass stalks, and the telescopic part of the second electric push rod (18) is fixedly connected to the top of the frame (17).

7. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation of claim 1, wherein, The top side of the processing box (10) is fixedly provided with a baffle (8) to prevent liquid on the straw stems from blowing onto the transparent door (2), and the baffle (8) is made of transparent material.

8. The isolated, sterile, disinfected, air-dried apparatus of grass culm and seed endophytic fungal isolation of claim 2, wherein, The bottom inner wall of the disinfection box (9) is fixedly provided with multiple partitions, and the disinfection box (9) is divided into six areas by the partitions. A net bag for placing seeds is detachably fixed on one side of the first clamp (15).

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