Stem cell subpackaging equipment

Abstract

The utility model discloses stem cell subpackaging equipment, and relates to the technical field of stem cell subpackaging. The device comprises a working table, two supporting plates which are symmetrically distributed are fixedly installed on the upper surface of the working table, a containing plate is fixedly installed at the top ends of the supporting plates, a split charging tank is arranged in the middle of the containing plate, a circular groove used in cooperation with the split charging tank is formed in the middle of the containing plate in a penetrating mode, and a clamping groove is formed in the middle of the circular groove. A liquid adding pipe is fixedly installed on one side of the subpackaging tank and located at the bottom of the subpackaging tank, a first electromagnetic valve is fixedly installed on the liquid adding pipe, a liquid outlet pipe is fixedly installed at the bottom end of the subpackaging tank, a second electromagnetic valve is fixedly installed on the liquid outlet pipe, and a culture dish is arranged below the liquid outlet pipe. A support frame is fixedly mounted at the top end of the split charging tank; according to the stem cell subpackaging device, the subpackaging quantity is consistent when stem cells are subpackaged every time, so that the influence on the research of the stem cells is avoided.

Description

Technical Field

[0001] This utility model relates to the field of stem cell dispensing technology, specifically a stem cell dispensing device. Background Technology

[0002] Stem cell aliquoting involves distributing cultured stem cell culture medium into different culture vessels for subsequent experiments or applications. When aliquoting stem cells, it is usually done by manually opening the cap of the culture medium storage bottle and inserting a pipette into the bottle to take the liquid, or by directly pouring the liquid from the bottle. This manual operation is time-consuming and makes it difficult to accurately control the amount of stem cells aliquoted, which affects stem cell research. Utility Model Content

[0003] To address the problem that manual operation is time-consuming and makes it difficult to accurately control the amount of stem cells dispensed, thus affecting stem cell research, the purpose of this invention is to provide a stem cell dispensing device.

[0004] To solve the above technical problems, this utility model adopts the following technical solution: a stem cell dispensing device, including a workbench, two symmetrically distributed support plates fixedly installed on the upper surface of the workbench, a placement plate fixedly installed at the top of the support plates, a dispensing tank provided in the middle of the placement plate, a circular groove for cooperating with the dispensing tank being opened through the middle of the placement plate, a liquid addition pipe fixedly installed on one side of the dispensing tank, the liquid addition pipe being located at the bottom of the dispensing tank, a first solenoid valve fixedly installed on the liquid addition pipe, a liquid outlet pipe fixedly installed at the bottom of the dispensing tank, a second solenoid valve fixedly installed on the liquid outlet pipe, a culture dish provided below the liquid outlet pipe; the top of the dispensing tank... A support frame is fixedly installed at one end. Two symmetrically distributed fixing blocks are fixedly installed on the inner wall of the support frame. A threaded rod rotatably passes through the middle of the fixing blocks. The threaded rod rotatably passes through the support frame. A threaded sleeve is threadedly connected to the outside of the threaded rod. A movable plate is fixedly installed on the side of the threaded sleeve away from the support frame. A limiting groove for cooperating with the threaded sleeve is opened on the side of the support frame near the movable plate. A connecting rod is fixedly installed at the bottom end of the movable plate. The connecting rod is slidably connected to the dispensing tank. The connecting rod is located at the middle position of the dispensing tank. A piston is fixedly installed at the bottom end of the connecting rod. A forward and reverse motor fixedly connected to the threaded rod is fixedly installed at the top end of the support frame.

[0005] Preferably, a first rotating shaft is rotatably passed through the middle of the worktable, a disc is fixedly installed at the top of the first rotating shaft, and a plurality of annular uniform placement grooves are opened at the top of the disc. A gear is fixedly installed on the outside of the first rotating shaft, a U-shaped frame is fixedly installed at the bottom of the worktable, a second rotating shaft is rotatably clamped on the lower surface of the worktable, both the second and first rotating shafts are rotatably connected to the U-shaped frame, a half-face gear for cooperating with the gear is fixedly installed on the outside of the second rotating shaft, and a motor is fixedly installed at the bottom of the U-shaped frame and fixedly connected to the second rotating shaft.

[0006] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0007] 1. This application ensures that the amount of stem cells dispensed is consistent each time, thereby avoiding any impact on stem cell research;

[0008] 2. This application enables the replacement of culture dishes containing and empty culture dishes while dispensing stem cells, thereby improving the efficiency of stem cell dispensing. Attached Figure Description

[0009] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0010] Figure 1 This is a schematic diagram of the structure of this utility model.

[0011] Figure 2 This is a schematic diagram of the placement plate and circular groove structure in this utility model.

[0012] Figure 3 This is a schematic diagram of the internal structure of the packaging tank in this utility model.

[0013] Figure 4 This is a schematic diagram of the internal structure of the support frame in this utility model.

[0014] Figure 5 This is a schematic diagram of the bottom structure of the workbench in this utility model.

[0015] Figure 6 This is a schematic diagram of the connection structure between the disc and the placement groove in this utility model.

[0016] In the diagram: 1. Workbench; 10. First solenoid valve; 11. Support plate; 12. Placement plate; 13. Dispensing tank; 14. Circular groove; 15. Liquid addition pipe; 16. Liquid outlet pipe; 17. Second solenoid valve; 18. Petri dish; 19. Controller; 2. Support frame; 20. Limiting groove; 21. Fixing block; 22. Threaded rod; 23. Threaded sleeve; 24. Moving plate; 25. Connecting rod; 26. Piston; 27. Forward and reverse motor; 3. First rotating shaft; 31. Disc; 32. Placement groove; 33. Gear; 34. U-shaped frame; 35. Second rotating shaft; 36. Half-face gear; 37. Motor; 4. Fixing plate. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: Figure 1-6 As shown, this utility model provides a stem cell dispensing device, including a workbench 1. Two symmetrically distributed support plates 11 are fixedly installed on the upper surface of the workbench 1. A placement plate 12 is fixedly installed at the top of the support plate 11. A dispensing tank 13 is provided in the middle of the placement plate 12. A circular groove 14 for cooperating with the dispensing tank 13 is opened through the middle of the placement plate 12. A liquid addition pipe 15 is fixedly installed on one side of the dispensing tank 13. The liquid addition pipe 15 is located at the bottom of the dispensing tank 13. A first solenoid valve 10 is fixedly installed on the liquid addition pipe 15. A liquid outlet pipe 16 is fixedly installed at the bottom of the dispensing tank 13. A second solenoid valve 17 is fixedly installed on the liquid outlet pipe 16. A culture dish 18 is provided below the liquid outlet pipe 16.

[0019] A support frame 2 is fixedly installed at the top of the dispensing tank 13. Two symmetrically distributed fixing blocks 21 are fixedly installed on the inner wall of the support frame 2. A threaded rod 22 is rotatably passed through the middle of the fixing block 21. The threaded rod 22 rotatably passes through the support frame 2. A threaded sleeve 23 is threadedly connected to the outside of the threaded rod 22. A movable plate 24 is fixedly installed on the side of the threaded sleeve 23 away from the support frame 2. A limiting groove 20 that cooperates with the threaded sleeve 23 is opened on the side of the support frame 2 close to the movable plate 24. A connecting rod 25 is fixedly installed at the bottom of the movable plate 24. The connecting rod 25 is slidably connected to the dispensing tank 13. The connecting rod 25 is located at the middle position of the dispensing tank 13. A piston 26 is fixedly installed at the bottom of the connecting rod 25. A forward and reverse motor 27 that is fixedly connected to the threaded rod 22 is fixedly installed at the top of the support frame 2.

[0020] A first rotating shaft 3 is rotatably connected through the middle of the workbench 1. A disc 31 is fixedly installed at the top of the first rotating shaft 3. Several annular and evenly spaced grooves 32 are opened at the top of the disc 31. A gear 33 is fixedly installed on the outside of the first rotating shaft 3. A U-shaped frame 34 is fixedly installed at the bottom of the workbench 1. A second rotating shaft 35 is rotatably mounted on the lower surface of the workbench 1. Both the second rotating shaft 35 and the first rotating shaft 3 are rotatably connected to the U-shaped frame 34. A half-face gear 36 that works with the gear 33 is fixedly installed on the outside of the second rotating shaft 35. A motor 37 that is fixedly connected to the second rotating shaft 35 is fixedly installed at the bottom of the U-shaped frame 34.

[0021] The height of the placement tank 32 should be lower than the height of the culture dish 18, and the diameter of the placement tank 32 should be the same as the diameter of the culture dish 18, so as to facilitate the placement of the culture dish 18 into the placement tank 32 and the removal of the culture dish 18 containing stem cells from the placement tank 32.

[0022] The dispensing container 13 is made of transparent material, making it easier to know how much liquid has been added to it, or approximately how much liquid has been added.

[0023] The bottom of the disc 31 does not fit against the top surface of the worktable 1 to avoid friction between the disc 31 and the worktable 1 when the disc 31 rotates with the first rotating shaft 3, which would cause wear on the top surface of the worktable 1 and the bottom surface of the disc 31 and affect the use of the disc 31.

[0024] The bottom of the dispensing tank 13 is truncated cone-shaped, and the bottom of the piston 26 is shaped to match the bottom of the dispensing tank 13, thus avoiding waste of the liquid entering the dispensing tank 13.

[0025] A controller 19 is fixedly installed at the top edge of the workbench 1. The controller 19 is electrically connected to the first solenoid valve 10, the second solenoid valve 17, the forward and reverse motor 27, and the motor 37. By setting the controller 19, it is easier to control the first solenoid valve 10, the second solenoid valve 17, the forward and reverse motor 27, and the motor 37 to close and open.

[0026] Both sides of the dispensing tank 13 are fixedly installed with fixing plates 4. The fixing plates 4 are fixedly connected to the placement plate 12 by bolts. When the dispensing tank 13 passes through the circular groove 14 on the placement plate 12, the fixing plates 4 prevent the dispensing tank 13 from falling directly into the circular groove 14, which would affect the installation of the dispensing tank 13. After the fixing plates 4 and the placement plate 12 are attached, the fixing plates 4 and the placement plate 12 are fixed together by bolts, thereby realizing the installation of the dispensing tank 13.

[0027] Working principle: In actual use, when adding liquid, the liquid addition pipe 15 is connected to other hoses, and the hoses are directly inserted into the storage bottle containing the culture medium and fixed, or the culture medium is poured into the dispensing tank 13. When liquid needs to be added to the dispensing tank 13, the first solenoid valve 10 needs to be opened. When liquid is drawn in through the hose, the piston 26 starts at the position of the liquid addition pipe 15, and then the controller 19 starts the forward and reverse motor 27, which drives the threaded rod 22 to rotate. Since the threaded sleeve 23 is limited by the limiting groove 20, the rotation of the threaded rod 22 drives the threaded sleeve 23 to move upward. The movement of the threaded sleeve 23 drives the moving plate 24, the connecting rod 25, and the piston 26 to move upward. When the piston 26 is pulled upward, the pressure in the dispensing tank 13 decreases, and the external atmospheric pressure draws the liquid in the storage bottle into the dispensing tank 13 through the hose and the liquid addition pipe 15.

[0028] When there is a certain amount of liquid in the dispensing tank 13, the forward and reverse motor 27 stops rotating and closes the first solenoid valve 10; when liquid is directly poured into the dispensing tank 13, the piston 26 is located at the top of the dispensing tank 13, and the second solenoid valve 17 is closed when liquid is added.

[0029] When dispensing is required, the flow rate of the second solenoid valve 17 is set via controller 19. Then, culture dishes 18 are placed in all the placement slots 32. Subsequently, the second solenoid valve 17 and the forward / reverse motor 27 are activated via controller 19. At this time, one culture dish 18 is located directly below the outlet pipe 16. The activation of the forward / reverse motor 27 drives the threaded rod 22 to reverse, causing the threaded sleeve 23, moving plate 24, and connecting rod 25 to move downwards. The pressure in the dispensing tank 13 increases, and liquid is ejected from the outlet pipe 16. During the liquid ejection process, when the second solenoid valve 17... When the solenoid valve 17 detects that the set flow rate has been reached, the reversible motor 27 stops, the second solenoid valve 17 closes, and at the same time, the motor 37 starts, driving the second rotating shaft 35 and the half-face gear 36 to rotate. When the half-face gear 36 meshes with the gear 33, it drives the first rotating shaft 3 and the disk 31 to rotate, so that the next culture dish 18 is located directly below the liquid outlet pipe 16. When the culture dish 18 is located directly below the liquid outlet pipe 16, the half-face gear 36 and the gear 33 are not meshed, and at the same time, the reversible motor 27 starts, the second solenoid valve 17 opens, and the liquid flows out.

[0030] When the culture dish 18 containing liquid reaches the position where it can be removed, the culture dish 18 containing liquid is picked up manually and an empty culture dish 18 is placed on top. During the replacement, the culture dish 18 located below the liquid outlet tube 16 is dispensing the liquid.

[0031] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A stem cell aliquoting apparatus comprising a worktable (1), characterized in that: Two symmetrically distributed support plates (11) are fixedly installed on the upper surface of the workbench (1). A placement plate (12) is fixedly installed at the top of the support plate (11). A dispensing tank (13) is provided in the middle of the placement plate (12). A circular groove (14) for use with the dispensing tank (13) is opened through the middle of the placement plate (12). A liquid addition pipe (15) is fixedly installed on one side of the dispensing tank (13). The liquid addition pipe (15) is located at the bottom of the dispensing tank (13). A first solenoid valve (10) is fixedly installed on the liquid addition pipe (15). A liquid outlet pipe (16) is fixedly installed at the bottom of the dispensing tank (13). A second solenoid valve (17) is fixedly installed on the liquid outlet pipe (16). A petri dish (18) is provided below the liquid outlet pipe (16). A support frame (2) is fixedly installed at the top of the dispensing tank (13). Two symmetrically distributed fixing blocks (21) are fixedly installed on the inner wall of the support frame (2). A threaded rod (22) is rotatably inserted through the middle of the fixing block (21). The threaded rod (22) rotatably inserts through the support frame (2). A threaded sleeve (23) is threadedly connected to the outer thread of the threaded rod (22). A movable plate (24) is fixedly installed on the side of the threaded sleeve (23) away from the support frame (2). The support frame (2) is close to... A limiting groove (20) for use with a threaded sleeve (23) is provided on one side of the movable plate (24). A connecting rod (25) is fixedly installed at the bottom end of the movable plate (24). The connecting rod (25) is slidably connected to the dispensing tank (13). The connecting rod (25) is located in the middle position of the dispensing tank (13). A piston (26) is fixedly installed at the bottom end of the connecting rod (25). A forward and reverse motor (27) fixedly connected to the threaded rod (22) is fixedly installed at the top end of the support frame (2).

2. A stem cell aliquoting device as claimed in claim 1, wherein, The workbench (1) has a first rotating shaft (3) that rotates through the middle. A disc (31) is fixedly installed at the top of the first rotating shaft (3). Several annular and uniformly spaced slots (32) are opened at the top of the disc (31). A gear (33) is fixedly installed on the outside of the first rotating shaft (3). A U-shaped frame (34) is fixedly installed at the bottom of the workbench (1). A second rotating shaft (35) is rotatably mounted on the lower surface of the workbench (1). The second rotating shaft (35) and the first rotating shaft (3) are rotatably connected to the U-shaped frame (34). A half-face gear (36) that cooperates with the gear (33) is fixedly installed on the outside of the second rotating shaft (35). A motor (37) that is fixedly connected to the second rotating shaft (35) is fixedly installed at the bottom of the U-shaped frame (34).

3. A stem cell sub-aliquoting device as claimed in claim 2, wherein, The height of the placement groove (32) is lower than the height of the petri dish (18), and the diameter of the placement groove (32) is the same as the diameter of the petri dish (18).

4. The stem cell aliquoting apparatus of claim 1, wherein, The dispensing container (13) is made of transparent material.

5. The stem cell aliquoting apparatus of claim 2, wherein, The bottom end of the disk (31) does not fit against the top surface of the workbench (1).

6. The stem cell dispensing device as described in claim 1, characterized in that, The bottom of the dispensing tank (13) is truncated cone-shaped, and the bottom shape of the piston (26) is consistent with the bottom shape of the dispensing tank (13).

7. The stem cell aliquoting device of claim 2, wherein, A controller (19) is fixedly installed at the top edge of the workbench (1). The controller (19) is electrically connected to the first solenoid valve (10), the second solenoid valve (17), the forward and reverse motor (27), and the motor (37).

8. The stem cell sub-packaging device of claim 1, wherein, Both sides of the dispensing tank (13) are fixedly installed with fixing plates (4), and the fixing plates (4) are fixedly connected to the placement plate (12) by bolts.

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