Flat plate bacterial colony cutting adapter

Abstract

The utility model discloses a plate bacterial colony cutting adapter, which relates to the technical field of fungus culture and comprises a cutting tube, a connecting rod, an elastic rod and a fixing block. The lower end of the cutting pipe is of a knife edge structure, the inner wall is rough, the cutting pipe is installed and connected to the front end of the rigid connecting rod, a rod body of the elastic rod is located above the connecting rod, and the tail end of the elastic rod and the tail end of the connecting rod are installed and connected with the fixing block. The front end of the rod body is bent downwards from the upper end opening of the cutting pipe to extend into the cutting pipe and is fixedly connected with a top ring located in the cutting pipe, and a notch is formed in the position, corresponding to the rod body, of the top of the side wall of the cutting pipe. The consistency of the diameter and the size of the transferred plate bacterial colony is controlled and ensured by the form of the cutting pipe, and meanwhile, the distance between the obtained bacterial colony area and the bacterial colony edge (the top end of the mycelium) can be controlled by adjusting the cutting position, so that the consistency of the cell age (culture time) and the mycelium uniformity (thin and dense) of the bacterial colony in the obtained area is ensured; and comparison between strains and varieties is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of fungal culture technology, specifically to a tool for cutting and transferring plate colonies. Background Technology

[0002] Colony selection for transfer is a common operation in the research, strain comparison, and variety selection of edible and medicinal fungi. Maintaining the same diameter, size, and age of the selected colony blocks is the basis for accurate identification and evaluation of the transferred strains.

[0003] Based on existing technology searches, the following known technical solutions exist:

[0004] Prior Art 1:

[0005] Early tools for extracting colonies of edible and medicinal fungi were conventional "T"-shaped punches.

[0006] However, the punch in the existing technology 1 requires the petri dish lid to be completely opened during actual operation, and the mycelium cake is punched and inoculated both above the petri dish, which is prone to contamination. At the same time, since there is no device to push the mycelium cake out, it is necessary to use tweezers or toothpicks to pick it off, which is inefficient.

[0007] Existing technology 2: Colony punch

[0008] Application number: CN202320346484.X, application date: 2023.03.01, publication (announcement) date: 2023.06.20.

[0009] Prior art 2 provides a punch with sampling and transfer functions to improve sampling efficiency. It includes an outer cylinder, an inner cylinder, a piston rod, a punch, and a release assembly. The inner cylinder is located inside the outer cylinder, and the piston is slidably disposed inside the inner cylinder. The inner cylinder has a driving component that moves the piston, and the piston has a needle shaft. One end of the punch is inserted into the space between the inner and outer cylinders, and the other end is the punching part. The release assembly is disposed in the interlayer between the inner and outer cylinders. By pressing the release assembly, the punch is pushed out from between the inner and outer cylinders. After sampling, the piston movement causes the needle shaft to contact the colonies inside the punch, facilitating the removal of the colonies. The release assembly allows for quick disassembly of the punch. When sampling different solid plates of the same strain, only the punch needs to be replaced.

[0010] Existing technology 3: Portable microbial perforator

[0011] Application number: CN201920173390.0, application date: 2019.01.31, publication (announcement) date: 2019.11.05.

[0012] Prior art 3 relates to a portable microbial perforator, comprising a support sleeve, a rotating disk with an open upper portion of the support sleeve, a rotating bearing fitted between the rotating disk and the support sleeve, a plurality of perforated tubes longitudinally passing through the inner cavity of the support sleeve, a return spring between the top of each perforated tube and the rotating disk, a pusher plate at the lower part of the inner cavity of each perforated tube, and a threaded push rod longitudinally passing through the inner cavity of each perforated tube to drive the pusher plate to move up and down. This invention not only has a simple and reasonable structural design, but is also easy to operate and use, facilitating the removal of mycelial cakes adhering to the perforated tubes, reducing the risk of mycelial cake contamination, and is highly efficient and convenient.

[0013] Both prior art 2 and prior art 3 have a punch with a push rod. The piston movement of the push rod pushes out the fungal cake punched by the punch, solving the problem of the fungal cake being difficult to remove. However, both have relatively complex structures, are not convenient to operate, and still require the petri dish lid to be fully opened and operated above the petri dish, which is prone to contamination.

[0014] Prior Art 4: A Novel Microbial Plate Puncher

[0015] Application number: CN202122669403.3, application date: 2021.11.03, publication (announcement) date: 2022.06.17.

[0016] Prior art 4 relates to a novel microbial plate puncher, comprising: a first elastic arm, a second elastic arm, a punching ring, a punching blade, and a sampler; the punching ring is disposed at a first end of the first elastic arm, and the punching blade is disposed at a first end axially of the punching ring; the sampler is disposed at a first end of the second elastic arm; and the second end of the first elastic arm is hinged to the second end of the second elastic arm; furthermore, the microbial puncher has a first state and a second state; in the first state, the sampler is separated from the punching ring; in the second state, the sampler extends partially or completely into the interior of the punching ring from the first end. This disclosure enables inoculation with the bacterial surface facing down, accelerates the growth rate of microorganisms on new culture media, improves the inoculation success rate, and significantly shortens the experimental cycle.

[0017] Existing technology 4 has a simple structure, and the hand is positioned to the side of the petri dish when holding it, allowing for inoculation with the mycelium facing downwards, which has certain advantages. However, existing technology 4 requires the two flexible arms to be opened and flipped significantly during use, which is very inconvenient in actual operation. The efficiency of removing, transferring, and inoculating the mycelium is low, and it is also prone to contamination by other microorganisms.

[0018] The above search results show that the above technical solutions do not affect the novelty of this utility model; and the combination of the above prior art does not destroy the inventiveness of this utility model. Utility Model Content

[0019] This invention provides a plate colony cutting adapter to avoid the shortcomings of the prior art.

[0020] The present invention adopts the following technical solution to solve the technical problem: a plate colony cutting adapter, wherein a cutting tube is installed and connected to the front end of a rigid connecting rod, the lower end of the cutting tube has a blade structure and a rough inner wall, and includes an elastic rod and a fixing block;

[0021] The body of the elastic rod is located above the connecting rod, and both its end and the end of the connecting rod are installed and connected to the fixing block;

[0022] The front end of the rod is bent downward from above the upper opening of the cutting tube and extends into the cutting tube, and is fixedly connected to a top ring located inside the cutting tube;

[0023] A notch is formed at the top of the side wall of the cutting tube corresponding to the position of the rod body. The notch provides clearance for the front end of the elastic rod to bend downwards until the top ring moves down inside the cutting tube to the bottom opening of the cutting tube.

[0024] Furthermore, the elastic rod is made of steel wire.

[0025] Furthermore, a button is installed between the rear and tail ends of the elastic rod.

[0026] Furthermore, the button is slidably fitted onto the rod, forming a sliding pair between them that supports the button sliding forward or backward along the rod.

[0027] Furthermore, this also includes locking screws;

[0028] The front end of the fixing block is provided with a connecting rod mounting hole and an elastic rod mounting hole; the rear ends of the connecting rod and the rod body are respectively inserted into the connecting rod mounting hole and the elastic rod mounting hole, and at least two locking screws are respectively threaded into the fixing block and tightened, and are respectively passed through the connecting rod mounting hole and the elastic rod mounting hole to abut against the end of the connecting rod and the end of the rod body.

[0029] Furthermore, the end of the rod has a hook structure with an opening facing its own front end.

[0030] Furthermore, a handle is also fixedly connected to the rear end of the fixing block.

[0031] Furthermore, a stabilizing handle is fixed to the bottom of the handle.

[0032] Furthermore, the front end of the connecting rod is welded and fixed to the cutting tube.

[0033] Furthermore, the front end of the connecting rod is provided with an external thread, and the side wall of the cutting tube is correspondingly fixed with a threaded tube;

[0034] The external thread is tightened into the threaded tube, and the connecting rod and the cutting tube are detachably installed and fixed.

[0035] This invention provides a plate colony cutting adapter, which has the following beneficial effects:

[0036] 1. The uniformity of the diameter and size of the plate colonies transferred by this utility model is ensured by the shape control of the cutting tube. At the same time, the distance between the taken colony area and the edge of the colony (hyphae tip) can be controlled by adjusting the cutting position, thereby ensuring the uniformity of the age (culture time) and the uniformity (sparse or dense) of the mycelium in the taken area, which is conducive to carrying out comparisons between strains and varieties.

[0037] 2. The present invention has a simple and reasonable structural design and is easy to operate and use. It can efficiently cut and transfer plate colonies, and is particularly suitable for the selection of superior strains of edible and medicinal fungi and the testing of the three characteristics of varieties. Attached Figure Description

[0038] Figure 1 This is a schematic diagram of the structure of this utility model;

[0039] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0040] Figure 3 Images of well-cultured plate colonies;

[0041] Figure 4 To preserve images of the empty plate colonies after cutting the selected colony area;

[0042] Figure 5 Image showing a round colony block being transferred to another spare plate medium;

[0043] In the picture:

[0044] 1. Cutting tube; 11. Notch; 12. Threaded tube; 2. Connecting rod; 21. External thread; 3. Elastic rod; 31. Rod body; 32. Top ring; 33. Button; 34. Hook structure; 4. Fixing block; 41. Connecting rod mounting hole; 42. Elastic rod mounting hole; 43. Locking screw; 5. Handle; 6. Stabilizing handle. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below in conjunction with the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0046] A plate colony cutting adapter, such as Figures 1-2 As shown, its structural relationship is as follows: the cutting tube 1 is installed and connected to the front end of the rigid connecting rod 2. The lower end of the cutting tube 1 has a knife-edge structure and a rough inner wall, including an elastic rod 3 and a fixing block 4.

[0047] The body 31 of the elastic rod 3 is located above the connecting rod 2, and its end and the end of the connecting rod 2 are both installed and connected to the fixing block 4;

[0048] The front end of the rod 31 bends downward from above the upper opening of the cutting tube 1 and extends into the cutting tube 1, and is fixedly connected to the top ring 32 located inside the cutting tube 1.

[0049] A notch 11 is made at the top of the side wall of the cutting tube 1 corresponding to the position of the rod 31. The notch 11 allows the front end of the elastic rod 3 to bend down to the top ring 32, which moves down inside the cutting tube 1 to the bottom opening of the cutting tube 1.

[0050] In actual setup, the cutting tube 1 is preferably a cylindrical tube, and the inner diameter can be determined according to the research purpose and requirements. When the connecting rod 2 is in a horizontal position, the cutting tube 1 is preferably in a vertical downward position with the bottom opening. When the rod body 31 is not under downward pressure, it is preferably in a position parallel to the connecting rod 2, and the highest point of its front end is flush with the top of the cutting tube 1. The outer diameter of the top ring 32 is preferably slightly smaller than the inner dimension of the cutting tube 1.

[0051] Preferably, the elastic rod 3 is a steel wire.

[0052] Preferably, a button 33 is installed between the rear and the tail end of the elastic rod 3.

[0053] Users can easily press down on the rod 31 by applying downward pressure to the button 33, causing the front end of the rod 31 to swing down and push the selected colony area obtained by cutting inside the cutting tube 1 downward from the lower end of the cutting tube 1, avoiding the inconvenience and hand discomfort caused by the hand directly acting on the thinner rod 31.

[0054] Preferably, the button 33 is slidably fitted onto the rod 31, forming a sliding pair between them to support the button 33 sliding forward or backward along the rod 31.

[0055] In actual use, various factors, including finger length and grip posture, can be taken into consideration. The button 33 can be slid back and forth along the rod 31 to a suitable position so that the user can apply downward pressure to the button 33 more conveniently according to their own situation.

[0056] Preferably, it also includes a locking screw 43;

[0057] The front end of the fixing block 4 is provided with a connecting rod mounting hole 41 and an elastic rod mounting hole 42; the rear ends of the connecting rod 2 and the rod body 31 are respectively inserted into the connecting rod mounting hole 41 and the elastic rod mounting hole 42, and at least two locking screws 43 are respectively threaded into the fixing block 4 and tightened, and respectively through the connecting rod mounting hole 41 and the elastic rod mounting hole 42 to abut against the end of the connecting rod 2 and the end of the rod body 31.

[0058] Preferably, the end of the rod 31 has a hook structure with an opening facing its own front end.

[0059] The rod 31 is usually a thin rod with a small diameter. The hook structure facilitates the locking screw 43 to tighten and limit the end of the rod 31.

[0060] Preferably, the rear end of the fixing block 4 is also fixedly connected to a handle 5.

[0061] In actual setup, the handle 5 is preferably coaxially arranged with the connecting rod 2.

[0062] Preferably, a stabilizing handle 6 is fixed to the bottom of the handle 5.

[0063] The stabilizing handle 6 plays a stabilizing role when the user performs colony transfer operations using the plate colony cutting adapter;

[0064] In actual setup, the stabilizing handle 6 is preferably positioned with its axis perpendicular to the axis of the handle 5; at the same time, the length of the stabilizing handle 6 should be as short as possible while ensuring that the hand can hold it firmly, so as to avoid interference between the stabilizing handle 6 and other structures, which would affect the ease of operation.

[0065] Preferably, the front end of the connecting rod 2 is welded and fixed to the cutting tube 1.

[0066] Preferably, the front end of the connecting rod 2 is provided with an external thread 21, and the side wall of the cutting tube 1 is correspondingly fixed with a threaded tube 12;

[0067] The external thread 21 is threaded and tightened into the threaded tube 12, allowing the connecting rod 2 and the cutting tube 1 to be detachably installed and fixed.

[0068] Example 1

[0069] like Figures 3-5As shown, plate colonies A represent Ganoderma lucidum, B represents shiitake mushroom, C represents wood ear fungus, and D represents oyster mushroom. The above-mentioned plate colony cutting adapter was used for colony cutting and transfer, including the following process:

[0070] The first step is to prepare plate culture medium.

[0071] Prepare PDA medium (20wt% potato, 2wt% glucose, 2wt% agar, water balance) using conventional methods, sterilize at 121℃ for 30 min, and then, under aseptic conditions, pour 20 mL of sterilized medium into each 90 mm petri dish, cover the dish, and cool to prepare plate culture medium for later use.

[0072] The second step is the plate colony culture of edible and medicinal fungi.

[0073] Under aseptic conditions, take the prepared plate culture medium and inoculate it with edible and medicinal fungi such as Ganoderma lucidum, shiitake mushroom, wood ear fungus, and oyster mushroom. Each treatment has more than 3 replicates. Under 25°C, the culture medium is incubated for about one week to obtain plate colonies of each strain.

[0074] The third step is to cut and connect the connectors.

[0075] Under aseptic conditions and in strict accordance with aseptic operation requirements, the operator selects the plate colonies to be transferred on the ultra-clean workbench, quickly opens the petri dish near the flame of an alcohol lamp, firmly grasps the handle 5 of the cutting adapter with the right hand, and holds the stabilizing handle 6 between the little finger and the ring finger. After the entire cutting tube 1 is sterilized and cooled by alcohol and the flame of an alcohol lamp, the bottom end of the cutting tube 1 is cut into the selected colony to separate the selected colony area from other colony areas, thus completing the cutting of the plate colony.

[0076] Then, open the lid of the spare plate culture dish, gently press button 33 on the rod 31 with your thumb to lower the front end of the rod 31, and move the top ring 32 down to the bottom inside the cutting tube 1. Push the cut circular colony block out from the bottom of the cutting tube 1 and transfer it to the spare plate culture dish. Cover the dish, release button 33, and the rod 31 will move the top ring 32 up and reset under its own elasticity, completing the cutting and transfer.

[0077] The fourth step is cultivation and calculation.

[0078] After transfer, the culture dishes are placed in a constant temperature incubator for cultivation. The cultivation temperature and number of days vary depending on the bacteria or are determined according to the test requirements. Once the requirements are met, the colony diameter is measured, and the mycelial growth rate is calculated.

[0079] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0080] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A plate colony cutting adapter, wherein a cutting tube (1) is installed and connected to the front end of a rigid connecting rod (2), the lower end of the cutting tube (1) has a blade-like structure and a rough inner wall, characterized in that: Includes an elastic rod (3) and a fixed block (4); The rod body (31) of the elastic rod (3) is located above the connecting rod (2), and its end and the end of the connecting rod (2) are both installed and connected to the fixing block (4); The front end of the rod (31) is bent downward from above the upper opening of the cutting tube (1) and extends into the cutting tube (1), and is fixedly connected to a top ring (32) located inside the cutting tube (1). A notch (11) is opened at the top of the side wall of the cutting tube (1) corresponding to the position of the rod (31). The notch (11) allows the front end of the elastic rod (3) to bend down to the top ring (32) inside the cutting tube (1) and reach the bottom opening of the cutting tube (1).

2. The colony picker of claim 1, wherein: The elastic rod (3) is a steel wire.

3. A flat plate colony cutting adapter according to claim 2, wherein: A button (33) is installed between the rear and the tail end of the elastic rod (3).

4. The colony cutter adapter of claim 3, wherein: The button (33) is slidably fitted onto the rod (31), and a sliding pair is formed between them to support the button (33) to slide forward or backward along the rod (31).

5. The colony cutter adapter of claim 1, wherein: It also includes locking screws (43); The front end of the fixing block (4) is provided with a connecting rod mounting hole (41) and an elastic rod mounting hole (42); the rear ends of the connecting rod (2) and the rod body (31) are respectively inserted into the connecting rod mounting hole (41) and the elastic rod mounting hole (42), and at least two locking screws (43) are respectively threaded into the fixing block (4) and are respectively inserted into the connecting rod mounting hole (41) and the elastic rod mounting hole (42) to abut against the end of the connecting rod (2) and the end of the rod body (31).

6. A flat plate colony cutting adapter according to claim 5, wherein: The end of the rod (31) has a hook structure with an opening facing its own front end.

7. The colony cutter adapter of claim 1, wherein: The rear end of the fixing block (4) is also fixedly connected to a handle (5).

8. The colony cutter adapter of claim 7, wherein: A stabilizing handle (6) is fixed to the bottom of the handle (5).

9. The colony cutter adapter of claim 1, wherein: The front end of the connecting rod (2) is welded and fixed to the cutting tube (1).

10. The colony picker of claim 1, wherein: The front end of the connecting rod (2) is provided with an external thread (21), and the side wall of the cutting tube (1) is correspondingly fixed with a threaded tube (12). The external thread (21) is threaded and fastened into the threaded tube (12), thereby detachably installing and fixing the connecting rod (2) and the cutting tube (1).

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