Perfusion device for preparing agarose gel

Abstract

The utility model provides a filling device for agarose gel preparation, which comprises a liquid adding tank, a plurality of gel preparing tanks and a liquid guide pipe communicated with the liquid adding tank and the gel preparing tanks, the gel preparing tanks are identical in specification, a liquid inlet hole is arranged at the center of the bottom of each gel preparing tank, the number of output ends of the liquid guide pipe is matched with that of the gel preparing tanks, and the liquid guide pipe is communicated with the liquid adding tank and the gel preparing tanks. The input end of the liquid guide pipe is connected with a liquid outlet hole formed in the center of the bottom of the liquid adding tank, prepared separation glue is arranged in the liquid adding tank, a pressing device is arranged on the upper portion of the liquid adding tank, an air blowing device is arranged on the side of the liquid adding tank, and a glass plate used for containing the separation glue is arranged in the glue making tank through a glass plate sealing sleeve. A sealing sleeve liquid inlet hole matched with the liquid inlet hole is formed in the bottom of the glass plate sealing sleeve, a certain amount of gas is injected into the sealed liquid adding groove through the air blowing device, and the separation gel is controlled to be stabilized at the fixed height of the glass plate through the pressing device.

Description

Technical Field

[0001] This utility model relates to the field of agarose gel preparation technology, and in particular to an infusion device for agarose gel preparation. Background Technology

[0002] Western blotting (Western immunoblotting) is commonly used to detect protein expression levels. Because proteins migrate different distances in SDS-PAGE under the same voltage conditions, different proteins can be separated on SDS-PAGE. Then, based on marker selection, a range close to the molecular weight of the target protein is chosen, and an electric field is used to transfer the selected proteins onto an NC or PVDF membrane. The transferred membrane is incubated with a specific antibody against the target protein. After membrane washing, the target protein remains bound to the antibody and is not washed away. Finally, the results are detected by film exposure or gel imaging. This method is highly sensitive and widely used.

[0003] In the preliminary preparation work of the experiment, the preparation of agarose gel is undoubtedly one of the important steps for electrophoresis.

[0004] When preparing agarose gel, a glass plate is typically pressed firmly onto a gel casting stand. Pure water is added until the liquid level is equal to the glass plate. After standing for 30 minutes, the liquid level is observed to determine if the glass plate and gel casting stand are correctly installed. The pure water between the glass plates is then drained, and the prepared separating gel is added using a pipette. Anhydrous ethanol or ultrapure water is then used for liquid sealing. After standing for a period of time, the sealing solution is poured out, a stacking gel is added, and a comb is placed inside. Once solidified, the preparation is complete.

[0005] However, if the glass plates and gel casting rack are not installed correctly during the preparation process, leakage will occur before the separating gel solidifies after being added. Since the separating gel needs to be added separately between the glass plates, human error and pipette error will cause the vertical height of the separating gel in each gel to be inconsistent, which may result in inconsistent protein development during electrophoresis. When a large number of agarose gels need to be prepared, adding the separating gel to each gel separately may cause the first prepared separating gel to solidify before it is liquid sealed due to excessive operation time. Utility Model Content

[0006] To address the aforementioned technical problems, a perfusion device for preparing agarose gels is provided, which enables the simultaneous preparation of multiple agarose gels, making the preparation process simpler and more efficient, and overcoming the deficiency of needing to assess the system's airtightness before preparation. The technical means employed in this invention are as follows:

[0007] A perfusion apparatus for preparing agarose gel includes a liquid addition tank, a gel preparation tank, and a liquid guide tube connecting the liquid addition tank and the gel preparation tank. The gel preparation tanks are multiple and of identical specifications, each with a liquid inlet at its center bottom. The number of output ends of the liquid guide tube matches the number of gel preparation tanks, and the input end of the liquid guide tube is connected to a liquid outlet at the center bottom of the liquid addition tank. The liquid addition tank contains a prepared separating gel. A pressing device is located at the top of the liquid addition tank, and an air-blowing device is located on the side of the liquid addition tank. A glass plate for containing the separating gel is placed in the gel preparation tank through a glass plate sealing sleeve. The bottom of the glass plate sealing sleeve has a sealing sleeve inlet hole that matches the liquid inlet hole. A certain amount of gas is injected into the sealed liquid addition tank through the air-blowing device, and the pressing device controls the separating gel to remain stable at a fixed height on the glass plate.

[0008] Furthermore, the pressing device includes a compression baffle and a compression rotating rod. The compression baffle is installed on the upper part of the liquid filling tank. The compression rotating rod includes a handle and a rotating rod. The handle is connected to the upper end of the rotating rod, and the rotating rod is connected to the compression baffle. A sealing rubber ring is provided on the bottom surface of the compression baffle.

[0009] Furthermore, the air-blowing device includes an air-blowing bladder and an air-blowing bladder docking sealing tube, and the output end of the air-blowing bladder is connected to the air inlet of the liquid filling tank through the air-blowing bladder docking sealing tube.

[0010] Furthermore, a sealing ring is provided at the liquid inlet and at the liquid outlet.

[0011] Furthermore, the glue-making tank has a length of 7-8cm, a width of 1-2cm, and a height of 10-11cm.

[0012] Furthermore, the radius of the liquid addition tank is 1-2 cm, and the height is 5-7 cm.

[0013] Furthermore, the diameter of the liquid inlet, liquid outlet, and air inlet of the liquid filling tank is 0.5-1cm.

[0014] Furthermore, the glass plate sealing sleeve is an elastic sealing sleeve with a length of 6-7cm, a width of 0.5-1cm, and a height of 13-15cm.

[0015] Furthermore, both the glue-making tank and the liquid-adding tank are made of transparent material.

[0016] This invention has the following advantages: By setting sealing rings at each interface, the pressure difference between different parts effectively prevents leakage during gel preparation; the device adds all prepared separating gels into the liquid addition tank at once, utilizing the principle of communicating vessels and increasing air pressure through an air bladder, while rotating the compression rod to force the separating gel into the gel preparation tank, achieving highly consistent separating gel preparation and promoting the reproducibility of the protein electrophoresis process; multiple agarose gels can be prepared at once, improving preparation efficiency and ensuring consistency, while also avoiding variations in agarose gel solidification time during prolonged operation. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a top view of the overall structure of this utility model.

[0019] Figure 2 This is a front view of the overall structure of this utility model.

[0020] Figure 3 This is a schematic diagram of the overall structure of this utility model from the left.

[0021] In the diagram: 1. Glue forming tank; 2. Fixing plate; 3. Liquid inlet; 4. Liquid guide tube; 5. Fixing baffle; 6. Liquid filling tank; 7. Compression rotating rod; 8. Liquid outlet; 9. Air inlet; 10. Airbag docking sealing tube; 11. Inflatable airbag; 12. Sealing ring; 13. Compression baffle; 14. Glue plate lifting hole; 15. Glass plate sealing sleeve; 16. Sealing sleeve liquid inlet. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0023] like Figures 1-3As shown in the figure, this utility model discloses an infusion device for preparing agarose gel, including a liquid addition tank 6, a gel preparation tank 1, and a liquid guide pipe 4 connecting the liquid addition tank and the gel preparation tank. There are multiple gel preparation tanks, and each gel preparation tank has the same specifications. Each gel preparation tank has a liquid inlet hole 3 at the center of its bottom. The number of output ends of the liquid guide pipe matches the number of gel preparation tanks. The input end of the liquid guide pipe is connected to a liquid outlet hole 8 at the center of the bottom of the liquid addition tank. The liquid addition tank contains prepared separating gel. A pressing device is provided on the upper part of the liquid addition tank. An air blowing device is provided on the side of the liquid addition tank. A glass plate for containing the separating gel is placed in the gel preparation tank through a glass plate sealing sleeve 15. The bottom of the glass plate sealing sleeve has a sealing sleeve inlet hole 16 that matches the liquid inlet hole. A certain amount of gas is injected into the sealed liquid addition tank through the air blowing device. The pressing device controls the separating gel to be stable at a fixed height on the glass plate.

[0024] The glass plate sealing sleeve is provided with a glue plate lifting hole 14. After the glue is made, the glue plate is lifted out by lifting the glue plate lifting hole.

[0025] In this embodiment, each glue-making tank is independently set up, and each glue-making tank is connected to the outer frame by a fixing piece 2. For ease of management, the outer frame is a cylindrical structure, and each glue-making tank is arranged in it at a preset interval. In this embodiment, they are arranged radially around the center of the cylindrical outer frame.

[0026] Similarly, the liquid filling tank is fixed to the outer shell of the liquid filling tank by the fixed baffle 5, and the fixed baffle plays a supporting role.

[0027] Furthermore, the pressing device includes a compression baffle 13 and a compression rotating rod 7. The compression baffle is installed on the upper part of the liquid filling tank. The compression rotating rod includes a handle and a rotating rod. The handle is connected to the upper end of the rotating rod, and the rotating rod is connected to the compression baffle. A sealing rubber ring is provided on the bottom surface of the compression baffle.

[0028] Specifically, as an optional implementation, the compression baffle has a two-layer structure. After adhesive injection, the upper layer is fixed to the inner wall of the filling tank, while the lower layer, with a sealing ring, is connected to the compression rotating rod. As the handle rotates, the lower compression baffle with the sealing ring is compressed downwards. There are several ways to fix the upper layer of the compression baffle to the inner wall of the filling tank. For example, to avoid creating pin holes / screw holes on the outer shell of the filling tank, corresponding pin holes / screw holes can be installed on the upper layer of the compression baffle. After adhesive injection, the upper layer of the compression baffle is installed in the corresponding position using a pin or bolt.

[0029] Furthermore, the air-blowing device includes an air-blowing bladder 11 and an air-blowing docking sealing tube 10, wherein the output end of the air-blowing bladder is connected to the air inlet of the liquid filling tank through the air-blowing docking sealing tube.

[0030] Furthermore, a sealing ring 12 is provided at the liquid inlet and a sealing ring is provided at the liquid outlet.

[0031] Furthermore, the glue-making tank has a length of 7-8cm, a width of 1-2cm, and a height of 10-11cm.

[0032] Furthermore, the radius of the liquid addition tank is 1-2 cm, and the height is 5-7 cm.

[0033] Furthermore, the diameter of the liquid inlet, liquid outlet, and air inlet 9 of the liquid filling tank is 0.5-1cm.

[0034] Furthermore, the glass plate sealing sleeve is an elastic sealing sleeve with a length of 6-7cm, a width of 0.5-1cm, and a height of 13-15cm.

[0035] Furthermore, both the glue-forming tank and the liquid-adding tank are made of transparent material. Specifically, they can be made of transparent acrylic or equivalent materials.

[0036] Furthermore, the liquid guide tube is connected to each component via an internal spiral. Specifically, the inner side of the liquid guide tube has threads, and the outer sides of the inlet and outlet holes have threads, which are screwed together.

[0037] In practice, the experimenter first places the glass plate into the glass plate sealing sleeve 15 and connects it to the liquid guide tube 4. The other end of the liquid guide tube 4 is connected to the liquid addition tank 6. The prepared separating adhesive is added to the liquid addition tank 6. According to the number of adhesive plates to be prepared, the liquid guide tube 4 is connected to the adhesive preparation tank 1. The unused liquid outlet ends are clamped with water-stop clamps. Then, the sealing ring 12 is placed into the liquid addition tank 6, and the compression baffle 13 is placed on top. Air is introduced into the liquid addition tank 6 using the air bladder 11, and then the sealing tube 10 is connected to the sealing bladder to seal it. After that, the compression rotating rod 7 is rotated to stabilize the separating adhesive at a fixed height on the glass plate. In this embodiment, the glass plate sealing sleeve is transparent. By applying pressure, the liquid level of the separating adhesive is kept 1-2 cm below the upper edge of the glass plate.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model.

Claims

1. A perfusion device for agarose gel preparation, characterized in that, The device includes a liquid addition tank, a glue preparation tank, and a liquid guide pipe connecting the liquid addition tank and the glue preparation tank. There are multiple glue preparation tanks, all with identical specifications. Each glue preparation tank has a liquid inlet hole at its bottom center. The number of output ends of the liquid guide pipe matches the number of glue preparation tanks. The input end of the liquid guide pipe is connected to a liquid outlet hole at the bottom center of the liquid addition tank. The liquid addition tank contains prepared separating glue. A pressing device is located at the top of the liquid addition tank, and an air blowing device is located on the side of the liquid addition tank. A glass plate for containing the separating glue is placed in the glue preparation tank through a glass plate sealing sleeve. The bottom of the glass plate sealing sleeve has a sealing sleeve inlet hole that matches the liquid inlet hole. A certain amount of gas is injected into the sealed liquid addition tank through the air blowing device, and the pressing device controls the separating glue to remain stable at a fixed height on the glass plate.

2. The perfusion device for agarose gel preparation according to claim 1, characterized in that, The pressing device includes a compression baffle and a compression rotating rod. The compression baffle is installed on the upper part of the liquid filling tank. The compression rotating rod includes a handle and a rotating rod. The handle is connected to the upper end of the rotating rod, and the rotating rod is connected to the compression baffle. A sealing rubber ring is provided on the bottom surface of the compression baffle.

3. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, The air-blowing device includes an air-blowing bladder and an air-bladder docking sealing tube. The output end of the air-blowing bladder is connected to the air inlet of the liquid filling tank through the air-bladder docking sealing tube.

4. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, A sealing ring is provided at the liquid inlet and at the liquid outlet.

5. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, The glue-making tank has a length of 7-8cm, a width of 1-2cm, and a height of 10-11cm.

6. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, The radius of the liquid addition tank is 1-2 cm and the height is 5-7 cm.

7. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, The diameter of the liquid inlet, liquid outlet, and air inlet of the liquid filling tank is 0.5-1cm.

8. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, The glass plate sealing sleeve is an elastic sealing sleeve with a length of 6-7cm, a width of 0.5-1cm, and a height of 13-15cm.

9. The perfusion apparatus for agarose gel preparation according to claim 1, wherein, Both the glue-making tank and the liquid-adding tank are made of transparent material.

Images