Biochemical substance analysis system

Abstract

A biochemical substance analysis system comprises a liquid storage module, a liquid use module and a liquid transfer module. The liquid use module comprises a first biochemical reaction assembly and a second biochemical reaction assembly; the pipetting module comprises a first pipetting assembly, a second pipetting assembly and a gas path assembly, the first pipetting assembly is communicated with the first biochemical reaction assembly through the gas path assembly, the second pipetting assembly is communicated with the second biochemical reaction assembly through the gas path assembly, and the first biochemical reaction assembly is communicated with the second biochemical reaction assembly through the gas path assembly. The first pipetting assembly can transfer liquid between the liquid storage module and the first biochemical reaction assembly, and the second pipetting assembly can transfer the liquid in the liquid storage module to the second biochemical reaction assembly; and the gas path assembly can transfer a reaction product in the first biochemical reaction assembly to the second biochemical reaction assembly. The biochemical substance analysis system provided by the utility model can realize various biochemical reaction processes, and has relatively high integration level and automation degree.

Description

Technical Field

[0001] This application relates to the field of biochemistry, and more particularly to a biochemical substance analysis system. Background Technology

[0002] In fields such as biology, chemistry, or medicine, such as gene sequencing, steps such as library construction, sample preparation, sample loading, and sequencing are usually required. These steps all involve the transfer of biochemical samples and reagents used.

[0003] However, most existing pipetting methods require manual operation, which is cumbersome and time-consuming. In addition to manual pipetting, there are also automated pipetting systems for liquid transfer, but these automated pipetting systems have complex tubing, are bulky, and require complex debugging of various components, which is not conducive to production assembly and product maintenance. Moreover, they are difficult to integrate with other equipment required for gene sequencing. Utility Model Content

[0004] In view of this, in order to solve at least one of the above defects, it is necessary to propose a biochemical substance analysis system.

[0005] This application provides a biochemical substance analysis system, including: a liquid storage module, a liquid usage module, and a pipetting module. The liquid storage module stores liquids required for biochemical reactions; the liquid usage module includes a first biochemical reaction component and a second biochemical reaction component; the pipetting module includes a first pipetting component, a second pipetting component, and a gas path component. The first pipetting component is connected to the first biochemical reaction component via the gas path component, the second pipetting component is connected to the second biochemical reaction component via the gas path component, and the first biochemical reaction component is connected to the second biochemical reaction component via the gas path component. Specifically, the first pipetting component is configured to transfer liquid between the liquid storage module and the first biochemical reaction component under the drive of the gas path component; the second pipetting component is configured to transfer liquid from the liquid storage module to the second biochemical reaction component under the drive of the gas path component; and the gas path component is configured to transfer reaction products from the first biochemical reaction component to the second biochemical reaction component.

[0006] In some possible embodiments, the first pipetting assembly includes: a moving mechanism, a first driving mechanism disposed on the moving mechanism, and a first liquid-taking structure disposed on the first driving mechanism. The first driving mechanism is configured to drive the first liquid-taking structure to move along a first direction, and the moving mechanism is configured to drive the first driving mechanism and the first liquid-taking structure to move along a second direction. The first direction is the length direction of the first liquid-taking structure, and the second direction is perpendicular to the first direction. The second pipetting assembly is arranged side by side with the first pipetting assembly along a third direction, which is perpendicular to the first direction and the second direction. The second pipetting assembly includes a second driving mechanism, a driving plate disposed on the second driving mechanism, and a second liquid-taking structure disposed on the driving plate. The second driving mechanism is configured to drive the driving plate, thereby driving the second liquid-taking structure to move along the first direction.

[0007] In some possible embodiments, the first liquid collection structure includes two first liquid collection needles arranged side by side along the third direction, and the first driving mechanism is configured to synchronously drive the two first liquid collection needles to move along the first direction.

[0008] In some possible embodiments, the second liquid collection structure includes a first liquid collection needle assembly, which includes a liquid collection needle fixing plate, a second liquid collection needle, and an elastic connection structure. The second liquid collection needle is fixedly connected to the liquid collection needle fixing plate and is movably inserted through the drive plate. The liquid collection needle fixing plate and the drive plate are elastically connected through the elastic connection structure, and the liquid collection needle fixing plate can move relative to the drive plate.

[0009] In some possible embodiments, the elastic connection structure includes: a connector and a first elastic member, the connector being movably inserted through the drive plate and connected to the liquid collection needle fixing plate, the first elastic member being located on the side of the drive plate away from the liquid collection needle fixing plate, the first elastic member being sleeved on the connector, and its two ends respectively abutting against the ends of the drive plate and the connector.

[0010] In some possible embodiments, the second liquid-collecting structure includes a first liquid-collecting needle assembly, which includes a buffer disposed on the drive plate and a second liquid-collecting needle disposed on the buffer. The buffer includes a sliding support portion disposed on the drive plate, a sliding guide portion passing through the sliding support portion along the first direction, a second elastic member disposed on one side of the sliding support portion and sleeved on the sliding guide portion, and a pressure plate disposed on the side of the sliding support portion away from the second elastic member. The two ends of the second elastic member respectively abut against the ends of the sliding support portion and the sliding guide portion. The second liquid-collecting needle passes through and is connected to the sliding guide portion. The sliding guide portion can drive the second liquid-collecting needle to slide relative to the sliding support portion along the first direction, thereby deforming the second elastic member.

[0011] In some possible embodiments, the second pipetting assembly further includes a positioning structure disposed on the drive plate.

[0012] In some possible embodiments, the pipetting module further includes a cleaning assembly, which includes a cleaning fluid needle, a cleaning drive mechanism communicating with the cleaning fluid needle, and a cleaning fluid distribution line, wherein the cleaning fluid distribution line is respectively connected to the cleaning drive mechanism, the first pipetting assembly, and the second pipetting assembly.

[0013] In some possible embodiments, the gas path assembly includes: a first gas path assembly and a second gas path assembly. The first gas path assembly includes a first reversing structure connected to the first pipetting assembly and a first power source connected to the first reversing structure. The first reversing structure is also connected to the first biochemical reaction assembly and the second biochemical reaction assembly. The second gas path assembly includes a second reversing structure connected to the second pipetting assembly and a second power source connected to the second reversing structure.

[0014] In some possible embodiments, the pipetting module further includes a mounting plate and a control component, wherein the first pipetting component and the second pipetting component are disposed side by side on the mounting plate; the control component is disposed on the side of the mounting plate and is configured to control the pipetting module to perform pipetting operations.

[0015] In the biochemical analysis system provided in this application embodiment, the pipetting module integrates the first pipetting component and the second pipetting component together, which improves the integration level, simplifies the structural complexity of the pipetting module, reduces the volume of the pipetting module, and facilitates the integration of the pipetting module into the biochemical analysis system. Through the cooperation of the pipetting module with the liquid storage module and the liquid use module, various biochemical reaction processes can be realized, such as sample preparation and gene sequencing reaction in gene sequencing, which effectively improves the integration level and automation level of the biochemical analysis system. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments of this application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a module architecture diagram of a biochemical substance analysis system provided in an embodiment of this application.

[0018] Figure 2 This is a module architecture diagram of a pipetting module provided in one embodiment of this application.

[0019] Figure 3 This is a schematic diagram of the structure of a pipetting module according to an embodiment of this application.

[0020] Figure 4 for Figure 3 A schematic diagram of the structure of the first pipetting assembly.

[0021] Figure 5 for Figure 3 A schematic diagram of the structure of the second pipetting assembly.

[0022] Figure 6 for Figure 5 A schematic diagram of the second pipetting assembly from another perspective.

[0023] Figure 7 This is a cross-sectional view of the first liquid collection needle assembly in one embodiment of this application.

[0024] Figure 8 This is a cross-sectional view of the first liquid collection needle assembly in another embodiment of this application.

[0025] Explanation of main component symbols

[0026]

[0027] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0028] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0029] It should be noted that when a component is described as "fixed to" or "mounted to" another component, it can be directly on the other component or may be interspersed with an intermediate component. When a component is described as "set to" another component, it can be directly set on the other component or may be interspersed with an intermediate component. The term "and / or" as used herein includes all and any combination of one or more of the associated listed items.

[0030] Please see Figure 1 As shown in the illustration, this application provides a biochemical substance analysis system 1000, which can be used for analytical processes such as reaction and detection of biochemical substances in fields such as biology, chemistry, or medicine, for example, in gene sequencing. The biochemical substance analysis system 1000 may include a pipetting module 100, a liquid storage module 200, and a liquid usage module 300. The liquid storage module 200 is used to store the liquid required for the biochemical reaction. This liquid may be, for example, a biological sample (such as a nucleic acid sample, human blood sample, tissue sample, or saliva sample), an adapter, reagents used in the biochemical analysis, or a mixture of biological samples, reagents, and sample carriers (such as magnetic beads), but is not limited to these. The pipetting module 100 is connected to the liquid usage module 300. The pipetting module 100 is configured to transfer liquid between the liquid storage module 200 and the liquid usage module 300. For example, liquid in the liquid storage module 200 can be transferred to the liquid usage module 300, or liquid in the liquid usage module 300 can be transferred to the liquid storage module 200.

[0031] Specifically, the liquid utilization module 300 may include two parts: a first biochemical reaction component 301 and a second biochemical reaction component 302. The first biochemical reaction component 301 is capable of sample preparation processes, such as the preparation of amplified products before gene sequencing. The second biochemical reaction component 302 is capable of detecting and analyzing biochemical substances, such as gene sequencing. After integrating the first biochemical reaction component 301 and the second biochemical reaction component 302 into the same device, how to achieve liquid transfer between the two reaction components becomes the research focus of this application.

[0032] Please see Figure 2 and Figure 3 As shown, please refer to the following: Figure 1As shown in the figure, this application provides an integrated pipetting module 100, which includes a first pipetting component 2, a second pipetting component 3, and a gas path component 4. Both the first pipetting component 2 and the second pipetting component 3 are connected to a liquid usage module 300. The gas path component 4 is connected to both the first pipetting component 2 and the second pipetting component 3, providing driving force for liquid transfer and adjusting the direction of liquid transfer. Specifically, the first pipetting component 2 is connected to a first biochemical reaction component 301 via the gas path component 4, and can be used to transfer liquid between the liquid storage module 200 and the first biochemical reaction component 301. The second pipetting component 3 is connected to a second biochemical reaction component 302 via the gas path component 4, and can be used to transfer liquid from the liquid storage module 200 to the second biochemical reaction component 302. In addition, the first biochemical reaction component 301 and the second biochemical reaction component 302 are connected through the gas path component 4, and the reaction products prepared by the first biochemical reaction component 301 can be transferred to the second biochemical reaction component 302 under the drive of the gas path component 4.

[0033] The pipetting module 100 also includes a mounting plate 1. A first pipetting assembly 2 and a second pipetting assembly 3 are mounted side-by-side on the mounting plate 1. The liquid storage module 200 can be located below the mounting plate 1. The first pipetting assembly 2 and the second pipetting assembly 3 can pass through the mounting plate 1 to achieve liquid intake or discharge. Specifically, the mounting plate 1 includes a first mounting area 11 and a second mounting area 12 arranged side-by-side. The first pipetting assembly 2 is mounted in the first mounting area 11, and the second pipetting assembly 3 is mounted in the second mounting area 12.

[0034] Please see Figure 4 As shown, please refer to the following: Figure 1 and Figure 2As shown, the first liquid transfer assembly 2 includes: a moving mechanism 21, a first driving mechanism 22 disposed on the moving mechanism 21, and a first liquid-taking structure 23 disposed on the first driving mechanism 22. The first driving mechanism 22 is configured to drive the first liquid-taking structure 23 to move along a first direction Z, allowing the first liquid-taking structure 23 to extend into or retract from the liquid storage module 200. Simultaneously, the first liquid-taking structure 23 is connected to the gas path assembly 4, and under the driving force of the gas path assembly 4, the first liquid-taking structure 23 can perform liquid taking and discharging. The moving mechanism 21 is configured to drive the first driving mechanism 22 and the first liquid-taking structure 23 to move along a second direction Y, thereby enabling the first liquid-taking structure 23 to transfer liquid to different positions within the liquid storage module 200. Here, the first direction Z is the length direction (or vertical direction) of the first liquid-taking structure 23, and the second direction Y is perpendicular to the first direction Z. For example, the liquid storage module 200 may include multiple holes arranged sequentially along the second direction Y, each hole containing a different liquid. The first liquid-taking structure 23 can move along the second direction Y under the drive of the moving mechanism 21, thereby transferring liquids in different holes. By moving the first liquid-taking assembly 2 along the second direction Y, the same first liquid-taking structure 23 can be used to transfer liquids in multiple holes, simplifying the structure of the first liquid-taking assembly 2 and reducing the complexity of the liquid-taking operation.

[0035] In some embodiments, the moving mechanism 21 includes a support frame 211 mounted on the mounting plate 1, a first driving member 212 mounted on the support frame 211, a first lead screw 213 mounted on the first driving member 212, a first slide rail 214 mounted on the support frame 211 and parallel to the first lead screw 213, and a first slider 215 mounted on the first slide rail 214. Both the first lead screw 213 and the first slide rail 214 extend along a second direction Y. The first driving mechanism 22 is mounted on the first slider 215. The first driving member 212 can drive the first lead screw 213 to rotate, thereby causing the first slider 215 to move along the first slide rail 214 to adjust the position of the first liquid-collecting structure 23.

[0036] In some embodiments, the first liquid-collecting structure 23 includes a plurality of first liquid-collecting needles 24 arranged side by side along a third direction X. For example, two first liquid-collecting needles 24 can be arranged side by side, with the third direction X perpendicular to the first direction Z and the second direction Y. The first driving mechanism 22 is configured to synchronously drive the plurality of first liquid-collecting needles 24 to move along the first direction Z to complete liquid collection and dispensing operations. By arranging the plurality of first liquid-collecting needles 24 side by side, at least two rows of liquid can be transferred. Furthermore, by synchronously driving the plurality of first liquid-collecting needles 24 up and down, the complexity of the driving structure of the first liquid-collecting needles 24 is simplified, further simplifying the structure of the first pipetting assembly 2. Utilizing the movement of the first pipetting assembly 2 in the second direction Y, liquid transfer to multiple orifices can be achieved. For example, using two first liquid-collecting needles can achieve liquid transfer operations to 22 orifices on the liquid storage module 200, saving 20 liquid-collecting needles and effectively simplifying the structural complexity and tubing connection complexity of the first pipetting assembly 2.

[0037] In some embodiments, the first driving mechanism 22 includes a lifting plate 221 disposed on a first slider 215, a second driving member 222 disposed on the lifting plate 221, a second lead screw 223 disposed on the second driving member 222, a second slide rail 224 disposed on the lifting plate 221 and parallel to the second lead screw 223, and a second slider 225 disposed on the second slide rail 224. The second lead screw 223 and the second slide rail 224 extend along a first direction Z. A plurality of first liquid-collecting needles 24 are mounted on the second slider 225. The second driving member 222 drives the second slider 225 to move along the second lead screw 223 and the second slide rail 224, thereby achieving synchronous lifting and lowering of the plurality of first liquid-collecting needles 24.

[0038] In some embodiments, the first mounting area 11 of the mounting plate 1 has an opening 13 corresponding to the first liquid extraction structure 23. The moving mechanism 21 is located on one side of the opening 13. During the movement of the first liquid extraction structure 23 along the first direction Z, the first liquid extraction needle 24 can pass through the opening 13 and extend into the liquid storage module 200 below the opening 13. Specifically, the length of the opening 13 can be substantially the same as the moving distance of the moving mechanism 21, or slightly greater than the moving distance of the moving mechanism 21.

[0039] Please see Figure 5 and Figure 6 As shown, please refer to the following: Figure 1 and Figure 2As shown, the second pipetting assembly 3 and the first pipetting assembly 2 are arranged side by side along a third direction X. The second pipetting assembly 3 includes a second driving mechanism 31, a driving plate 32 disposed on the second driving mechanism 31, and a second liquid-taking structure 33 disposed on the driving plate 32. The second driving mechanism 31 is configured to drive the driving plate 32, thereby driving the second liquid-taking structure 33 to move along a first direction Z, so that the second liquid-taking structure 33 can extend into or exit the liquid storage module 200. At the same time, the second liquid-taking structure 33 is connected to the gas path assembly 4. Under the driving force of the gas path assembly 4, the second liquid-taking structure 33 can realize liquid taking and discharging. Specifically, the driving plate 32 is located above the mounting plate 1. The second driving mechanism 31 is mounted on the mounting plate 1 through a driving shaft 38. The second mounting area 12 of the mounting plate 1 has multiple through holes 14 corresponding to the second liquid-taking structure 33. The second driving mechanism 31 can drive the second liquid-taking structure 33 to pass through the through holes 14 and extend into or exit the liquid storage module 200 below the mounting plate 1 to realize liquid taking or discharging.

[0040] The second liquid extraction structure 33 may include multiple sets of extraction needles to achieve different liquid transfer functions. Specifically, the second liquid extraction structure 33 may include a first extraction needle group 34, a second extraction needle group 35, and a third extraction needle group 36, wherein the first extraction needle group 34 can be used to transfer a first liquid, the second extraction needle group 35 can be used to transfer a second liquid, and the third extraction needle group 36 can be used to transfer a third liquid.

[0041] Please see Figures 5 to 7 As shown, please refer to the following: Figure 1 and Figure 2 As shown, the first liquid collection needle assembly 34 can be an elastically buffered liquid collection needle, capable of elastic liquid collection. Specifically, it includes: a liquid collection needle fixing plate 341, at least one second liquid collection needle 342, and an elastic connecting structure 343. The second liquid collection needle 342 is fixedly connected to the liquid collection needle fixing plate 341 and movably passes through the drive plate 32. The liquid collection needle fixing plate 341 and the drive plate 32 are elastically connected through the elastic connecting structure 343. The liquid collection needle fixing plate 341 can move relative to the drive plate 32. During the relative movement, the elastic connecting structure 343 can deform, thereby achieving the elastic connection between the liquid collection needle fixing plate 341 and the drive plate 32.

[0042] The elastic connection structure 343 includes a connector 344 and a first elastic member 345. The connector 344 movably passes through the drive plate 32 and is connected to the liquid collection needle fixing plate 341. The first elastic member 345 is located on the side of the drive plate 32 away from the liquid collection needle fixing plate 341. The first elastic member 345 is sleeved on the connector 344, and its two ends respectively abut against the ends of the drive plate 32 and the connector 344.

[0043] The principle of the first liquid-collecting needle assembly 34 for elastic liquid collection is as follows: Driven by the second driving mechanism 31, the driving plate 32 moves the first liquid-collecting needle assembly 34 downwards along the first direction Z. When the second liquid-collecting needle 342 contacts the bottom of the liquid storage module 200, the bottom of the liquid storage module 200 applies a reverse force upwards along the first direction Z to the second liquid-collecting needle 342. This reverse force pushes the liquid-collecting needle fixing plate 341 relative to the driving plate 32 upwards along the first direction Z, compressing the first elastic element 345. The compressive elastic force generated by the deformation of the first elastic element 345 ensures that the second liquid-collecting needle 342 makes tight contact with the bottom of the liquid storage module 200, allowing for sufficient liquid absorption and effectively reducing the dead volume of the fluid. It also avoids damage to the second liquid-collecting needle 342 due to rigid contact. After liquid absorption, driven by the second driving mechanism 31, the driving plate 32 moves the first liquid-collecting needle assembly 34 upwards along the first direction Z, allowing the first elastic element 345 to recover its deformation and the first liquid-collecting needle assembly 34 to reset. The first liquid-collecting needle group 34, which is capable of elastically absorbing liquid, can collect the liquid at the bottom of the liquid storage module 200, minimizing liquid residue. This method of liquid aspiration is particularly effective in reducing the waste of precious liquids, especially for liquids required for some precious biochemical reactions.

[0044] In some embodiments, the first elastic element 345 may be a compression spring.

[0045] In some embodiments, the second liquid-collecting needle 342 includes a liquid-collecting needle body 346 and a sleeve 347 sleeved on the outside of the liquid-collecting needle body 346, with a gap between the sleeve 347 and the liquid-collecting needle body 346. The gas passage assembly 4 can communicate with the liquid-collecting needle body 346 to achieve liquid collection and drainage. The gap can communicate with the cleaning fluid box of the liquid storage module 200 to achieve cleaning of the outer surface of the liquid-collecting needle body 346.

[0046] Understandably, in other embodiments, please refer to Figure 8 As shown, please refer to the following: Figure 5As shown, this application embodiment also provides another first liquid-dispensing needle assembly 34a. The first liquid-dispensing needle assembly 34a can adopt other elastic buffer modes to achieve elastic liquid dispensing. Specifically, the first liquid-dispensing needle assembly 34a includes: a buffer 39 disposed on the drive plate 32 and a second liquid-dispensing needle 342 disposed on the buffer 39. The buffer 39 includes: a sliding support portion 391 disposed on the drive plate 32, a sliding guide portion 392 disposed through the sliding support portion 391 along a first direction Z, a second elastic member 393 disposed on one side of the sliding support portion 391 and sleeved on the sliding guide portion 392, and a pressure plate 394 disposed on the side of the sliding support portion 391 away from the second elastic member 393. The two ends of the second elastic member 393 abut against the ends of the sliding support portion 391 and the sliding guide portion 392, respectively. The second liquid-collecting needle 342 passes through and connects to the sliding guide portion 392. The sliding guide portion 392 can drive the second liquid-collecting needle 342 to slide relative to the sliding support portion 391 along the first direction Z, thereby causing the second elastic element 393 to deform. Specifically, the principle of using the buffer 39 to achieve elastic liquid collection is as follows: when the first liquid-collecting needle assembly 34a is driven to move downward along the first direction Z, when the second liquid-collecting needle 342 contacts the bottom of the liquid storage module 200, the bottom of the liquid storage module 200 will apply a reverse force upward along the first direction Z to the second liquid-collecting needle 342. This reverse force will push the second liquid-collecting needle 342 and the sliding guide portion 392 to move upward relative to the sliding support portion 391, so as to compress the second elastic element 393. The compressive elastic force generated by the deformation of the second elastic element 393 can make the second liquid-collecting needle 342 in close contact with the bottom of the liquid storage module 200, which can fully absorb liquid, effectively reduce the dead volume of fluid residue, and at the same time avoid damage to the second liquid-collecting needle 342 due to rigid contact. After the liquid aspiration is completed, the drive plate 32, driven by the second drive mechanism 31, drives the first liquid aspiration needle group 34a to move upward along the first direction Z, so that the second elastic member 393 restores its deformation and the first liquid aspiration needle group 34a is reset.

[0047] Please refer to it again. Figure 5 and Figure 6 As shown, the second sampling needle assembly 35 may include multiple third sampling needles 351, wherein the structure of the third sampling needles 351 may be substantially the same as that of the second sampling needles 342. Exemplarily, the second sampling needle assembly 35 can be used for reagents required in the gene transfer sequencing process. It is understood that the second sampling needle assembly 35 can also be used to discharge waste liquid.

[0048] Please refer to it again. Figure 5 and Figure 6 As shown, the third collection needle group 36 may include multiple fourth collection needles 361. The difference between the fourth collection needles 361 and the second collection needles 342 is that the fourth collection needles 361 are not equipped with cannulas. Exemplarily, the third collection needle group 36 can be used for primers required in the gene transfer sequencing process.

[0049] Please refer to it again. Figure 5 and Figure 6 Please refer to the following: Figure 1 and Figure 2 As shown, the pipetting module 100 also includes a cleaning component 5, which includes a cleaning fluid needle 51, a cleaning drive mechanism 52, and a cleaning fluid distribution line 53. The cleaning fluid needle 51 and the cleaning drive mechanism 52 are both mounted on the drive plate 32, and the cleaning drive mechanism 52 is connected to the cleaning fluid needle 51. The cleaning fluid distribution line 53 is connected to the cleaning drive mechanism 52, the first liquid-taking structure 23, and the second liquid-taking structure 33. Driven by the cleaning drive mechanism 52, the cleaning fluid in the liquid storage module 200 can be transferred through the cleaning fluid needle 51 to the first liquid-taking structure 23 and the second liquid-taking structure 33 via the cleaning fluid distribution line 53, thereby cleaning the first liquid-taking structure 23 and the second liquid-taking structure 33.

[0050] In some embodiments, the first liquid-taking needle 24 in the first liquid-taking structure 23 needs to be cleaned between the transfer of different liquids. The first liquid-taking structure 23 can be moved into the cleaning hole provided on the liquid storage module 200 by the moving mechanism 21. A branch of the cleaning liquid distribution pipeline 53 is connected to the first liquid-taking needle 24, thereby injecting the cleaning liquid into the first liquid-taking needle 24 to clean the inner wall and the lower outer wall of the first liquid-taking needle 24.

[0051] In some embodiments, the cleaning methods of the second liquid-collecting needle 342 and the third liquid-collecting needle 351 in the second liquid-collecting structure 33 are different from those of the first liquid-collecting needle 24. Taking the second liquid-collecting needle 342 as an example, the sleeve 347 of the second liquid-collecting needle 342 is connected to a branch of the cleaning fluid distribution pipeline 53. The cleaning fluid is injected into the gap between the sleeve 347 and the liquid-collecting needle body 346 through the cleaning drive mechanism 52 and the cleaning fluid needle 51, thereby cleaning the outer wall of the liquid-collecting needle body 346.

[0052] Please refer to it again. Figure 4 and Figure 5 As shown, the second pipetting assembly 3 also includes a positioning structure 37, which is disposed on the drive plate 32 and can be moved to the mounting plate 1. It can be used to cooperate with the positioning hole on the liquid storage module 200 to realize the positioning of the pipetting module 100.

[0053] Please refer to it again. Figure 1 and Figure 2As shown, the gas path assembly 4 may include a first gas path assembly 41. The first gas path assembly 41 includes a first reversing structure 43 connected to the first liquid intake structure 23 and a first power source 44 connected to the first reversing structure 43. In some embodiments, the first power source 44 may include various types of power sources for driving liquid movement, such as syringe pumps, plunger pumps, diaphragm pumps, gear pumps, and peristaltic pumps. The first reversing structure 43 may include various types of reversing structures for selecting the direction of fluid flow, such as solenoid valves, rotary valves, etc.

[0054] The gas path assembly 4 may further include a second gas path assembly 42. The second gas path assembly 42 includes a second reversing structure 45 communicating with the second liquid intake structure 33 and a second power source 46 communicating with the second reversing structure 45. In some embodiments, the second power source 46 may include various types of power sources for driving liquid movement, such as syringe pumps, plunger pumps, diaphragm pumps, gear pumps, and peristaltic pumps. The second reversing structure 45 may include various types of reversing structures for selecting the direction of fluid flow, such as solenoid valves, rotary valves, etc.

[0055] Please see Figure 2 and Figure 3 As shown, the pipetting module 100 also includes a control component 6, which is located on the side of the mounting plate 1. The control component 6 is connected to the first pipetting component 2, the second pipetting component 3 and the gas path component 4 respectively to coordinate the control of the above components to achieve pipetting transfer.

[0056] In the biochemical analysis system 1000 provided in this application embodiment, the pipetting module 100 integrates the first pipetting component 2 and the second pipetting component 3 together, which improves the integration level, simplifies the structural complexity of the pipetting module 100, reduces the volume of the pipetting module 100, and facilitates the integration of the pipetting module 100 into the biochemical analysis system 1000. Through the cooperation of the pipetting module 100 with the liquid storage module 200 and the liquid use module 300, various biochemical reaction processes can be realized, such as sample preparation and gene sequencing reaction in gene sequencing, which effectively improves the integration level and automation level of the biochemical analysis system 1000.

[0057] Furthermore, the components of the pipetting module 100 require no special adjustments, facilitating assembly and maintenance. The pipetting operation is simple and highly efficient, effectively avoiding inconsistencies caused by manual pipetting and improving the accuracy of biochemical analysis. Additionally, by utilizing the movement of the first pipetting component 2 in the second direction Y, pipetting operations can be performed on multiple wells of the liquid storage module 200. This allows for fully automated transfer of various liquids using a smaller number of needles, saving a significant number of needles and further simplifying the structural complexity of the pipetting module 100 and the complexity of its tubing design.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.

Claims

1. A biochemical substance analysis system characterized by comprising: include: Liquid storage module, used to store liquids required for biochemical reactions; The liquid utilization module includes a first biochemical reaction component and a second biochemical reaction component; as well as The pipetting module includes a first pipetting component, a second pipetting component, and a gas path component. The first pipetting component is connected to a first biochemical reaction component via the gas path component. The second pipetting component is connected to a second biochemical reaction component via the gas path component. The first biochemical reaction component is connected to the second biochemical reaction component via the gas path component. The first pipetting assembly is configured to transfer liquid between the liquid storage module and the first biochemical reaction assembly under the drive of the gas path assembly; the second pipetting assembly is configured to transfer liquid in the liquid storage module to the second biochemical reaction assembly under the drive of the gas path assembly; and the gas path assembly is configured to transfer reaction products in the first biochemical reaction assembly to the second biochemical reaction assembly.

2. The biochemical substance analysis system according to claim 1, wherein The first pipetting assembly includes: a moving mechanism, a first driving mechanism disposed on the moving mechanism, and a first liquid-taking structure disposed on the first driving mechanism. The first driving mechanism is configured to drive the first liquid-taking structure to move along a first direction, and the moving mechanism is configured to drive the first driving mechanism and the first liquid-taking structure to move along a second direction. The first direction is the length direction of the first liquid-taking structure, and the second direction is perpendicular to the first direction. The second pipetting assembly is arranged side by side with the first pipetting assembly along a third direction, which is perpendicular to the first direction and the second direction. The second pipetting assembly includes a second driving mechanism, a driving plate disposed on the second driving mechanism, and a second liquid-taking structure disposed on the driving plate. The second driving mechanism is configured to drive the driving plate, thereby causing the second liquid-taking structure to move along the first direction.

3. The biochemical substance analysis system according to claim 2, wherein The first liquid collection structure includes two first liquid collection needles arranged side by side along the third direction, and the first driving mechanism is configured to synchronously drive the two first liquid collection needles to move along the first direction.

4. The biochemical analysis system of claim 2, wherein The second liquid collection structure includes a first liquid collection needle assembly, which includes a liquid collection needle fixing plate, a second liquid collection needle, and an elastic connection structure. The second liquid collection needle is fixedly connected to the liquid collection needle fixing plate and is movably inserted through the drive plate. The liquid collection needle fixing plate and the drive plate are elastically connected through the elastic connection structure, and the liquid collection needle fixing plate can move relative to the drive plate.

5. The biochemical substance analysis system according to claim 4, wherein The elastic connection structure includes a connector and a first elastic member. The connector movably passes through the drive plate and is connected to the liquid collection needle fixing plate. The first elastic member is located on the side of the drive plate away from the liquid collection needle fixing plate. The first elastic member is sleeved on the connector, and its two ends respectively abut against the ends of the drive plate and the connector.

6. The biochemical analysis system of claim 2, wherein The second liquid-collecting structure includes a first liquid-collecting needle assembly, which includes a buffer disposed on the drive plate and a second liquid-collecting needle disposed on the buffer. The buffer includes: a sliding support portion disposed on the drive plate, a sliding guide portion passing through the sliding support portion along the first direction, a second elastic member disposed on one side of the sliding support portion and sleeved on the sliding guide portion, and a pressure plate disposed on the side of the sliding support portion away from the second elastic member. The two ends of the second elastic member respectively abut against the ends of the sliding support portion and the sliding guide portion. The second liquid-taking needle passes through and is connected to the sliding guide portion. The sliding guide portion can drive the second liquid-taking needle to slide relative to the sliding support portion along the first direction, so as to deform the second elastic member.

7. The biochemical substance analysis system according to claim 2, wherein The second pipetting assembly further includes a positioning structure disposed on the drive plate.

8. The biochemical substance analysis system according to claim 1, wherein The pipetting module further includes a cleaning component, which includes a cleaning needle, a cleaning drive mechanism connected to the cleaning needle, and a cleaning fluid distribution line. The cleaning fluid distribution line is connected to the cleaning drive mechanism, the first pipetting component, and the second pipetting component.

9. The biochemical substance analysis system as claimed in claim 1, wherein, The gas path assembly includes: A first gas path assembly includes a first reversing structure connected to the first pipetting assembly and a first power source connected to the first reversing structure. The first reversing structure is also connected to the first biochemical reaction assembly and the second biochemical reaction assembly. The second gas path assembly includes a second reversing structure connected to the second pipetting assembly and a second power source connected to the second reversing structure.

10. The biochemical substance analysis system as claimed in claim 1, wherein, The pipetting module also includes: Mounting plate, the first pipetting assembly and the second pipetting assembly are arranged side by side on the mounting plate; and A control component is located on the side of the mounting plate, and the control component is configured to control the pipetting module to perform pipetting operations.

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