Cell culture device for biological research and development

Abstract

The utility model provides a cell culture device for biological research and development, which relates to the technical field of auxiliary devices for biological experiments and comprises a main body, a culture bin is arranged in the main body and used for placing a culture dish for culturing cells, and an upper cover for sealing the culture bin is arranged at the top of the main body. According to the device, through the pneumatic control assembly, the pressure and composition of gas in the culture bin can be accurately adjusted, cell growth environments under different physiological conditions are simulated, and the survival rate and growth efficiency of cells are improved. The design of one-way air holes ensures one-way flowing of air, unnecessary air exchange is avoided, controllability of the system is enhanced, external pollution risks are reduced, the temperature control assembly enables temperature distribution in the culture bin to be more uniform, local overheating or supercooling is avoided, and it is ensured that cells grow in a stable temperature environment. The electric heating tubes are distributed at equal intervals, temperature changes can be responded rapidly, it is guaranteed that the temperature reaches and maintains a set value rapidly, and the requirement for rapid temperature adjustment or constant-temperature experiments is met.

Description

Technical Field

[0001] This utility model relates to the technical field of auxiliary devices for biological experiments, specifically cell culture devices for biological research and development. Background Technology

[0002] Cell culture is a key technology in biomedical research, drug development, and tissue engineering. Providing a stable and controllable environment is crucial for cell growth, differentiation, and functional expression. An ideal cell culture device needs to precisely control parameters such as temperature, gas composition, and humidity to simulate the physiological conditions of cells in vivo. With the development of biotechnology, the requirements for cell culture devices are becoming increasingly stringent, demanding not only a sterile environment but also the ability to adapt to various cell types and experimental needs.

[0003] Despite the progress made in existing cell culture equipment, some shortcomings remain. First, many traditional cell culture devices often cannot precisely regulate the gas pressure and composition within the culture chamber, which limits research on cell culture of different cell types or under specific physiological conditions. In addition, the temperature control system may lack uniformity or have excessively high heating efficiency, especially when rapid temperature adjustment or maintenance of a constant temperature is required, and existing equipment may not be able to meet these requirements. Utility Model Content

[0004] The present invention aims to address the shortcomings of the prior art by providing a cell culture device for biological research and development, thereby improving the deficiencies of existing devices in controlling gas and temperature for different cell cultures.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell culture device for biological research and development, comprising: a main body, wherein a culture chamber is provided inside the main body for placing culture dishes for culturing cells, and a top cover is provided on the top of the main body for sealing the culture chamber; a gas control component, wherein the gas control component is disposed in the main body on one side of the culture chamber for controlling the airtightness and reducing air to assist in culturing cells inside the culture chamber; and a temperature control component, wherein the temperature control component is disposed on one side of the main body for changing and controlling the temperature inside the culture chamber to improve the applicability of the device.

[0006] Furthermore, the gas control component includes: an air extraction chamber located inside the main body, with air extraction pipes provided on one side of the top and one side of the bottom of the air extraction chamber, and the air extraction pipe located on the top side of the air extraction chamber communicating with the interior of the culture chamber, and each air extraction pipe being equipped with a solenoid valve; a drive motor located on the inner wall of the bottom of the air extraction chamber, with a drive rod driven and connected to the top of the drive motor via a drive shaft; and an air extraction plate slidably connected inside the air extraction chamber, with the air extraction plate and the drive rod being threadedly engaged.

[0007] Furthermore, the gas control component also includes: air vents located on both sides of the main body, with an air vent pipe connected to the inside of the culture chamber on one side of the top of the air vent; an air inlet pipe located on one side of the top of the air vent, with an air pump installed inside the air inlet pipe; and a water exchange pipe located at the top of the air vent, with the air vent filled with water.

[0008] Furthermore, the temperature control component includes: a temperature-controlled water tank located on one side of the main body, a temperature-controlled pipe located on one side of the temperature-controlled water tank, and the temperature-controlled pipe passing through the main body and arranged in a threaded structure around the culture chamber; and a circulation pump located inside the temperature-controlled water tank, the circulation pump being connected to the temperature-controlled pipe.

[0009] Furthermore, the exhaust plate has two one-way air holes inside, and a sealing plate is provided inside the one-way air holes. A sealing ball is provided at the bottom of the sealing plate, and a spring is provided between the sealing plate and the sealing ball.

[0010] Furthermore, the temperature-controlled water tank is equipped with multiple electric heating tubes, which are equidistantly distributed among each other.

[0011] This invention provides a cell culture device for biological research and development, which has the following beneficial effects:

[0012] The advantages of this invention are that, through the gas control components, the device can precisely adjust the gas pressure and composition within the culture chamber, simulating cell growth environments under different physiological conditions, thereby improving cell survival rate and growth efficiency. The unidirectional vent design ensures unidirectional gas flow, avoiding unnecessary gas exchange, enhancing system controllability, and reducing the risk of external contamination.

[0013] Secondly, the temperature control components ensure a more uniform temperature distribution within the culture chamber, preventing localized overheating or undercooling and ensuring cell growth in a stable temperature environment. The evenly spaced heating elements respond rapidly to temperature changes, ensuring the temperature quickly reaches and maintains the set value, meeting the needs of experiments requiring rapid temperature adjustments or constant temperature control. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0015] Figure 2 This is a cross-sectional view of the overall structure of this utility model.

[0016] Figure 3 This is a schematic diagram of the air extraction plate structure of this utility model.

[0017] Figure 4 This is a schematic diagram of the temperature control tube structure of this utility model.

[0018] Figure 5 For the present utility model Figure 2 Enlarged diagram of point A in the diagram.

[0019] Figure 1-5 In the middle: 1-Main body; 101-Cultivation chamber; 102-Ventilation chamber; 103-Ventilation pipe; 104-Air inlet pipe; 105-Air inlet pump; 106-Water exchange pipe; 107-Top cover; 2-Ejection chamber; 201-Ejection pipe; 202-Drive motor; 203-Drive rod; 204-Ejection plate; 205-One-way vent; 206-Sealing plate; 207-Sealing ball; 208-Spring; 3-Temperature-controlled water tank; 301-Temperature control pipe; 302-Circulation pump; 303-Heating element. Detailed Implementation

[0020] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0021] The following disclosure provides many different embodiments or examples for implementing different structures of this application. To simplify the disclosure, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the scope of this application. Furthermore, reference numerals and / or letters may be repeated in different examples; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed. In addition, various specific examples of processes and materials are provided in this application, but those skilled in the art will recognize the application of other processes and / or the use of other materials.

[0022] This application provides a cell culture device for biological research and development. This device, through a gas control component, can precisely adjust the gas pressure and composition within the culture chamber, simulating cell growth environments under different physiological conditions, thereby improving cell survival rate and growth efficiency. The unidirectional vent design ensures unidirectional gas flow, avoiding unnecessary gas exchange, enhancing system controllability, and reducing the risk of external contamination. The temperature control component ensures a more uniform temperature distribution within the culture chamber, preventing localized overheating or undercooling and ensuring cell growth in a stable temperature environment. The evenly distributed heating elements can quickly respond to temperature changes, ensuring the temperature rapidly reaches and maintains the set value, meeting the needs of rapid temperature adjustment or isothermal experiments. The following provides a detailed description of this cell culture device for biological research and development. It should be noted that the order of description in the following embodiments is not intended to limit the preferred order of the embodiments.

[0023] The present application will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0024] Example 1

[0025] Please see Figure 1-5 This embodiment provides a cell culture device for biological research and development, comprising: a main body 1, with a culture chamber 101 inside the main body 1 for placing culture dishes for culturing cells, and a top cover 107 on the top of the main body 1 for sealing the culture chamber 101; a gas control component, disposed inside the main body 1 on one side of the culture chamber 101, for controlling the airtightness and reducing air to assist in culturing cells inside the culture chamber 101; and a temperature control component, disposed on one side of the main body 1, for changing and controlling the temperature inside the culture chamber 101 to improve the applicability of the device;

[0026] During use, the sealing effect of the top cover 107 can effectively prevent external contaminants from entering the culture chamber 101, ensuring a sterile environment during cell culture. This stable structure helps maintain the stability of the culture environment and reduces the impact of external factors on cell growth.

[0027] Furthermore, the pneumatic control component includes: an air extraction chamber 2 located inside the main body 1, with air extraction pipes 201 opened on one side of the top and one side of the bottom of the air extraction chamber 2, and the air extraction pipe 201 located on the top side of the air extraction chamber 2 communicating with the interior of the culture chamber 101, and each air extraction pipe 201 is equipped with a solenoid valve; a drive motor 202 located on the inner wall of the bottom of the air extraction chamber 2, with a drive rod 203 driven and connected to the top of the drive motor 202 via a drive shaft; and an air extraction plate 204 slidably connected inside the air extraction chamber 2, with the air extraction plate 204 and the drive rod 203 being threadedly engaged.

[0028] During use, the drive motor 202 drives the drive rod 203 to rotate via the drive shaft, thereby causing the suction plate 204 to move up and down to extract gas. The suction pipe 201 connects the culture chamber 101 and the suction chamber 2. The solenoid valve controls the flow of gas. In this process, firstly, by opening the suction chamber 2 and driving the suction plate 204 downward via the drive motor 202, the space above the suction pipe 201 increases, and the air below the suction plate 204 is expelled, reducing the amount of air inside the culture chamber 101. Secondly, the solenoid valve is closed, and the suction plate 204 is moved upward, returning to the top. By continuously cycling through these two steps, most of the air inside the culture chamber 101 can be extracted, thereby reducing the amount of air inside the culture chamber 101 and purifying the culture environment. This gas control helps improve cell survival rate and growth efficiency, supporting more complex experimental needs.

[0029] Furthermore, the pneumatic control component also includes: a ventilation chamber 102 located on both sides of the main body 1, with a ventilation pipe 103 connected to the inside of the culture chamber 101 on one side of the top of the ventilation chamber 102; an air inlet pipe 104 located on one side of the top of the ventilation chamber 102, with an air pump 105 installed inside the air inlet pipe 104; and a water exchange pipe 106 located at the top of the ventilation chamber 102, with the ventilation chamber 102 filled with water.

[0030] During use, when ventilation is required, the air pump 105 can pump outside air into the water in the ventilation chamber 102. At this time, the air can be filtered by the water, and the excess gas can enter the culture chamber 101 through the ventilation pipe 103, thereby preventing dust in the air from entering the interior. This provides a cleaner gas source for cells that need to be cultured with air.

[0031] Furthermore, the exhaust plate 204 has two one-way vents 205 inside, and a sealing plate 206 is provided inside the one-way vent 205. A sealing ball 207 is provided at the bottom of the sealing plate 206, and a spring 208 is provided between the sealing plate 206 and the sealing ball 207. During use, when the exhaust plate 204 moves upward, the one-way vents 205 open, and the sealing ball 207 is pushed away by the spring 208, allowing gas to be discharged; conversely, it prevents outside air from entering. In this way, it is ensured that gas can only be discharged from top to bottom, preventing the reverse entry of gas.

[0032] Example 2

[0033] Based on Example 1, the temperature control component includes: a temperature-controlled water tank 3 located on one side of the main body 1, a temperature-controlled pipe 301 located on one side of the temperature-controlled water tank 3, and the temperature-controlled pipe 301 passing through the main body 1 and arranged in a threaded structure around the culture chamber 101; and a circulation pump 302 located inside the temperature-controlled water tank 3, which is connected to the temperature-controlled pipe 301.

[0034] During use, the liquid in the temperature-controlled water tank 3 is distributed in a spiral shape around the culture chamber 101 through the temperature control pipe 301. The circulation pump 302 promotes the circulation of the liquid and evenly transfers heat. Under the action of the spirally arranged temperature control pipe 301 and the circulation pump 302, the temperature distribution in the culture chamber 101 is more uniform, avoiding local overheating or overcooling.

[0035] Furthermore, the temperature-controlled water tank 3 is equipped with multiple electric heating tubes 303, which are equidistantly distributed among each other. During use, the electric heating tubes 303 can heat the water inside the temperature-controlled water tank 3, thereby transferring heat to the cells inside the culture chamber 101 through the water. This provides a suitable culture temperature when cells require constant or elevated temperature culture.

[0036] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments.

[0037] The above provides a detailed description of a cell culture device for biological research and development provided in the embodiments of this application. Specific examples have been used to illustrate the principles and implementation methods of this application. The description of the above embodiments is only for the purpose of helping to understand the technical solutions and core ideas of this application. Those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A cell culture device for biological research and development, characterized in that, include: The main body (1) has a culture chamber (101) inside for placing culture dishes for culturing cells, and the top of the main body (1) has a cover (107) for sealing the culture chamber (101). A gas control component, disposed within the main body (1) on one side of the culture chamber (101), is used to control the airtightness and reduce airflow to assist in the culture of cells inside the culture chamber (101). The gas control component includes: An air extraction chamber (2) is provided inside the main body (1). An air extraction pipe (201) is provided on one side of the top and one side of the bottom of the air extraction chamber (2). The air extraction pipe (201) located on the top side of the air extraction chamber (2) is connected to the inside of the culture chamber (101). An electromagnetic valve is provided inside the air extraction pipe (201). A drive motor (202) is provided on the inner wall of the bottom of the air extraction chamber (2), and a drive rod (203) is connected to the top of the drive motor (202) via a drive shaft. A suction plate (204) is slidably connected inside the suction chamber (2), and the suction plate (204) is threadedly engaged with the drive rod (203); The pneumatic control component also includes: Ventilation chambers (102) are opened on both sides inside the main body (1), and a ventilation pipe (103) is connected to the inside of the culture chamber (101) on one side of the top of the ventilation chamber (102). An air inlet pipe (104) is provided on one side of the top of the air vent (102), and an air pump (105) is provided inside the air inlet pipe (104). A water exchange pipe (106) is provided at the top of the venting chamber (102), and the venting chamber (102) is filled with water; and A temperature control component is located on one side of the main body (1) and is used to change and control the temperature inside the culture chamber (101) to improve the applicability of the device.

2. The cell culture device for biological research and development according to claim 1, characterized in that, The temperature control component includes: A temperature-controlled water tank (3) is provided on one side of the main body (1), and a temperature control pipe (301) is provided on one side of the temperature-controlled water tank (3), and the temperature control pipe (301) passes through the main body (1) and is arranged in a spiral structure around the culture chamber (101); A circulation pump (302) is installed inside the temperature-controlled water tank (3), and the circulation pump (302) is connected to the temperature control pipe (301); The temperature-controlled water tank (3) is equipped with multiple electric heating tubes (303), which are equidistantly distributed among each other.

3. The cell culture device for biological research and development according to claim 1, characterized in that, The air extraction plate (204) has two one-way air holes (205) inside. The one-way air holes (205) are provided with a sealing plate (206) inside. The bottom of the sealing plate (206) is provided with a sealing ball (207). A spring (208) is provided between the sealing plate (206) and the sealing ball (207).

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