Escherichia coli constant-temperature fermentation device with built-in heat exchange spiral pipe

Abstract

The utility model discloses an Escherichia coli constant-temperature fermentation device with a built-in heat exchange spiral pipe, which relates to the field of Escherichia coli fermentation, and is characterized by comprising a fermentation tank, an inner cavity for Escherichia coli fermentation and an outer cavity designed around the inner cavity are arranged in the fermentation tank, a spiral heat exchange pipe is arranged in the outer cavity, and the spiral heat exchange pipe is arranged in the outer cavity. An inlet part of the spiral heat exchange pipe extends out of the top of the fermentation tank, an outlet part of the spiral heat exchange pipe extends out of the side end of the fermentation tank, and a gap between the outer cavity and the spiral heat exchange pipe is filled with a heat conduction material. Accurate control of the temperature of the fermentation inner cavity is achieved, complex heat changes in the fermentation process are effectively handled, and a stable constant-temperature environment is provided for growth and metabolism of escherichia coli.

Description

Technical Field

[0001] This utility model relates to the field of Escherichia coli fermentation, and more specifically, it relates to an Escherichia coli constant temperature fermentation device with an internal heat exchange spiral tube. Background Technology

[0002] Currently, the use of E. coli for fermentation to produce amino acids, enzymes, and recombinant proteins is widespread in fields such as biopharmaceuticals and the food industry. However, the growth and metabolism of E. coli are highly temperature-sensitive, and the cell growth and metabolite synthesis during fermentation are easily affected by temperature. Temperature fluctuations not only inhibit cell activity but also reduce the yield and quality of the target products.

[0003] In existing E. coli fermentation devices, the common jacketed heat exchange structure has insufficient heat exchange area and long heat transfer path, resulting in slow temperature control response and difficulty in coping with complex heat changes during fermentation. Some devices that use spiral heat exchange tubes have gaps between the spiral heat exchange tubes and the tank body, which are filled with low thermal conductivity substances such as air, forming thermal resistance, which greatly reduces the heat exchange efficiency and makes it impossible to maintain the constant temperature environment required for fermentation.

[0004] Therefore, in order to solve the above-mentioned technical problems, this application proposes an Escherichia coli isothermal fermentation device with an internal heat exchange spiral tube. Utility Model Content

[0005] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide an Escherichia coli constant temperature fermentation device with an internal heat exchange spiral tube.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a constant temperature fermentation device for Escherichia coli with an internal heat exchange spiral tube, comprising a fermenter, wherein the fermenter has an inner cavity for Escherichia coli fermentation and an outer cavity designed around the inner cavity, wherein a spiral heat exchange tube is disposed inside the outer cavity, wherein the inlet of the spiral heat exchange tube extends from the top of the fermenter and the outlet extends from the side of the fermenter, and the gap between the outer cavity and the spiral heat exchange tube is filled with a heat-conducting material.

[0007] Preferably, the fermenter is equipped with a top cover, and multiple feed pipes are embedded and fixed inside the top cover. The feed pipes are connected to different raw material storage tanks, and each of the multiple feed pipes is also equipped with an independent flow control valve and a metering device.

[0008] Preferably, a bearing is embedded and fixed in the center of the fermenter, which supports the rotation of the rotating rod. A stirring blade is welded to the outer wall of the rotating rod, and a motor that drives the rotating rod is installed at the top of the bearing.

[0009] Preferably, the stirring blade has a through groove, which allows the fermentation broth to form a more complex fluid movement path during stirring, increases the degree of turbulence, promotes more thorough mixing of the fermentation broth and nutrients, and enables E. coli to contact and absorb nutrients more efficiently.

[0010] Preferably, the outer wall of the fermenter is connected to the base via a bracket, and the base is located at the rear of the fermenter and is equipped with a lifting assembly for driving the top cover switch.

[0011] Preferably, the lifting assembly includes an electric guide rail fixed to the base, and a slider is slidably connected on the electric guide rail, the surface of which is connected to the top cover via a connecting plate.

[0012] Preferably, the thermally conductive material is metal powder, ceramic particles, or graphene.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. The heat exchange medium of this invention flows in from the top inlet of the spiral heat exchange tube and flows meanderingly along the spiral path within the outer cavity. It efficiently transfers heat through the heat-conducting material and the outer cavity wall, thereby heating or cooling the E. coli fermentation environment within the inner cavity. The heat-conducting material tightly fills the gaps, rapidly and evenly conducting the heat from the spiral heat exchange tube to the outer cavity wall, and then from the outer cavity wall to the inner cavity. This avoids the thermal resistance problem caused by gaps in traditional devices and the drawbacks of jacketed heat exchange structures, achieving precise control of the fermentation cavity temperature. It effectively copes with the complex heat changes during fermentation and provides a stable constant temperature environment for the growth and metabolism of E. coli, thus solving the problems mentioned in the background art.

[0015] 2. In this invention, when the top cover is placed in the fermentation tank, the feed pipe is connected to the inner cavity of the fermentation tank, thereby forming multiple feed channels. Each channel is connected to different raw material storage tanks and is equipped with an independent flow control valve and metering device, which can accurately control the feeding time and flow rate of different nutrients, provide a more suitable nutritional environment for the growth of E. coli, and improve fermentation efficiency.

[0016] 3. This invention features an automatic lifting mechanism that drives the top cover opening and closing, eliminating the need for manual labor to open and close the heavy top cover, thus reducing the workload of operators and making it particularly suitable for large fermenters. Furthermore, mechanized operation allows for faster opening and closing of the top cover, saving time on fermenter maintenance, cleaning, and material addition, thereby improving overall production efficiency. Attached Figure Description

[0017] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the specific structure of the side of this utility model;

[0020] Figure 3 This is a schematic diagram of the top cover connection structure in this utility model;

[0021] Figure 4 This utility model Figure 3 Enlarged view of the local structure of A;

[0022] Figure 5 This is a schematic diagram of the specific structure of the fermenter in this utility model.

[0023] In the diagram: 1. Fermentation tank; 101. Inner cavity; 102. Outer cavity; 103. Spiral heat exchange tube; 2. Top cover; 3. Feed pipe; 4. Flow control valve; 5. Metering device; 6. Bearing; 7. Rotary rod; 8. Stirring blade; 801. Through groove; 9. Motor; 10. Support; 11. Base; 12. Lifting assembly; 1201. Electric guide rail; 1202. Sliding block; 1203. Connecting plate; 13. Raw material storage tank. Detailed Implementation

[0024] like Figure 1-5 As shown, this utility model provides an Escherichia coli constant temperature fermentation device with an internal heat exchange spiral tube, including a fermenter 1. The fermenter 1 has an inner cavity 101 for Escherichia coli fermentation and an outer cavity 102 designed around the inner cavity 101. A spiral heat exchange tube 103 is arranged inside the outer cavity 102. The inlet of the spiral heat exchange tube 103 extends from the top of the fermenter 1, and the outlet extends from the side of the fermenter 1. The gap between the outer cavity 102 and the spiral heat exchange tube 103 is filled with a heat-conducting material.

[0025] This invention features an outer cavity 102 surrounding an inner cavity 101 within a fermenter 1, with a spiral heat exchange tube 103 arranged within the outer cavity 102. The spiral heat exchange tube 103, coiled in a spiral shape around the outer cavity 102, significantly increases the contact area with the heat exchange medium compared to a jacketed heat exchange structure where the heat exchange area is only formed on the outer side of the tank, resulting in more efficient heat exchange. From a heat transfer path perspective, the jacketed heat exchange structure has a long heat transfer path, leading to low efficiency and slow response in heat transfer to the inner cavity 101 of the fermenter 1. In contrast, the spiral heat exchange tube 103 is directly arranged within the outer cavity 102, allowing the heat exchange medium to transfer heat more quickly and closely to the outer cavity 102 wall via a thermally conductive material, thereby acting on the inner cavity 101 and enabling faster temperature control. Furthermore, the gap between the spiral heat exchange tube 103 and the outer cavity 102 is filled with thermally conductive material, eliminating the air thermal resistance between the traditional spiral tube and the tank. During operation, the heat exchange medium flows in from the top inlet of the spiral heat exchange tube 103 and flows meanderingly along the spiral path within the outer cavity 102. It efficiently transfers heat through the heat-conducting material and the wall of the outer cavity 102, thereby heating or cooling the E. coli fermentation environment in the inner cavity 101. The heat-conducting material tightly fills the gaps, rapidly and evenly conducting the heat from the spiral heat exchange tube 103 to the wall of the outer cavity 102, and then from the wall of the outer cavity 102 to the inner cavity 101. This avoids the thermal resistance problem caused by gaps in traditional devices and the drawbacks of jacketed heat exchange structures, achieving precise control of the temperature of the fermentation inner cavity 101, effectively coping with the complex heat changes during fermentation, and providing a stable constant temperature environment for the growth and metabolism of E. coli.

[0026] The thermally conductive material can be metal powder, ceramic particles, or graphene.

[0027] The top of the fermentation tank 1 is equipped with a top cover 2, and multiple feed pipes 3 are embedded and fixed inside the top cover 2. The feed pipes 3 are respectively connected to different raw material storage tanks 13 (the raw material storage tanks 13 are equipped with threaded caps for feeding). Each of the multiple feed pipes 3 is also equipped with an independent flow control valve 4 and a metering device 5. A bearing 6 is embedded and fixed in the center of the fermentation tank 1. The bearing 6 supports the rotation of the rotating rod 7. A stirring blade 8 is welded on the outer wall of the rotating rod 7. A motor 9 that drives the rotating rod 7 is installed at the top of the bearing 6.

[0028] When the top cover 2 is placed in the fermentation tank 1, the feed pipe 3 is connected to the inner cavity 101 of the fermentation tank 1, thereby forming multiple feed channels 801. Each channel is connected to different raw material storage tanks 13 and is equipped with an independent flow control valve 4 and a metering device 5, which can accurately control the feeding time and flow rate of different nutrients, providing a more suitable nutritional environment for the growth of E. coli and improving fermentation efficiency. At this time, the rotating rod 7 and its stirring blades 8 are also placed in the inner cavity 101. The rotating rod 7 is driven to rotate by the motor 9, and the rotating rod 7 is supported by the bearing 6. The rotating rod 7 drives the stirring blades 8 to rotate, thereby promoting full contact between E. coli and nutrients, thereby improving the fermentation yield.

[0029] Furthermore, the stirring blade 8 is provided with a through groove 801. On the one hand, the through groove 801 allows the fermentation broth to form a more complex fluid movement path during stirring, increasing the degree of turbulence and promoting more thorough mixing of the fermentation broth and nutrients, enabling E. coli to contact and absorb nutrients more efficiently. On the other hand, the through groove 801 reduces the contact area between the stirring blade 8 and the fermentation broth, reducing the resistance during stirring, thereby reducing the energy consumption of the motor 9 and improving stirring efficiency. Moreover, the outer wall of the fermentation tank 1 is connected to the base 11 via a bracket 10. The base 11 is located at the rear of the fermentation tank 1 and is equipped with a lifting assembly 12 that drives the top cover 2 to open and close. In this way, the lifting assembly 12 can automatically drive the top cover 2 to open and close, eliminating the need for manual labor to open and close the heavy top cover 2, reducing the labor intensity of operators, which is especially suitable for large fermentation tanks 1. At the same time, mechanized operation can complete the opening and closing of the top cover 2 more quickly, saving time for fermentation tank 1 maintenance, cleaning, and material addition operations, and improving overall production efficiency.

[0030] The following is the specific structure of the lifting assembly 12: The lifting assembly 12 includes an electric guide rail 1201 fixed to the base 11, and a slider 1202 is slidably connected on the electric guide rail 1201. The surface of the slider 1202 is connected to the top cover 2 through the connecting plate 1203. That is, the electric guide rail 1201 drives the slider 1202 to move up and down, and the slider 1202 drives the top cover 2 to move up and down through the connecting plate 1203, thereby realizing the opening and closing of the top cover 2.

[0031] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model in any way. Those skilled in the art can readily implement this utility model based on the accompanying drawings and the above description. However, any modifications, alterations, or equivalent variations made by those skilled in the art without departing from the scope of the utility model's technical solution, utilizing the disclosed technical content, are considered equivalent embodiments of this utility model. Furthermore, any equivalent changes, alterations, or variations made to the above embodiments based on the essential technology of this utility model are still within the protection scope of this utility model's technical solution.

Claims

1. A constant-temperature fermentation device for Escherichia coli with an internal heat exchange spiral tube, comprising a fermenter (1), characterized in that, The fermenter (1) has an inner cavity (101) for E. coli fermentation and an outer cavity (102) designed around the inner cavity (101). A spiral heat exchange tube (103) is installed inside the outer cavity (102). The inlet of the spiral heat exchange tube (103) extends from the top of the fermenter (1) and the outlet extends from the side of the fermenter (1). The gap between the outer cavity (102) and the spiral heat exchange tube (103) is filled with heat-conducting material.

2. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 1, characterized in that: The fermentation tank (1) is equipped with a top cover (2) and multiple feed pipes (3) are embedded inside the top cover (2). The feed pipes (3) are connected to different raw material storage tanks (13). The multiple feed pipes (3) are also equipped with independent flow control valves (4) and metering devices (5).

3. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 1, characterized in that: A bearing (6) is embedded in the center of the fermenter (1), and the bearing (6) supports the rotation of the rotating rod (7). A stirring blade (8) is welded on the outer wall of the rotating rod (7), and a motor (9) is installed at the top of the bearing (6) to drive the rotating rod (7).

4. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 3, characterized in that: The stirring blade (8) has a through groove (801).

5. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 2, characterized in that: The outer wall of the fermentation tank (1) is connected to the base (11) by a bracket (10). The base (11) is located behind the fermentation tank (1) and is equipped with a lifting assembly (12) that drives the top cover (2) to switch.

6. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 5, characterized in that: The lifting assembly (12) includes an electric guide rail (1201) fixed to the base (11), and a slider (1202) is slidably connected on the electric guide rail (1201). The surface of the slider (1202) is connected to the top cover (2) through a connecting plate (1203).

7. The Escherichia coli constant-temperature fermentation device with an internal heat exchange spiral tube according to claim 1, characterized in that: The thermally conductive material is metal powder, ceramic particles, or graphene.

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