Liquid chromatographic column

Abstract

The utility model relates to a liquid chromatographic column, which comprises a column tube filled with filler, openings at two ends of the column tube are provided with mounting heads, each mounting head comprises an inner tube inserted on the inner side of the column tube and an outer tube sleeved on the outer side of the column tube, a sealing ring is arranged on the peripheral wall of the inner tube, and a sieve plate is arranged on one side of a limit ring far away from the column tube. A pressing sleeve is arranged on the side, away from the limiting ring, of the sieve plate, the outer circumferential wall of the pressing sleeve is in threaded connection with the inner circumferential wall of the inner pipe, a liquid guide hole coaxial with the column pipe is further formed in the pressing sleeve, an axial sliding guide piece is arranged between the outer pipe and the column pipe, and a threaded sleeve is rotationally connected to the end, away from the connecting ring, of the outer pipe and is in threaded connection with the outer circumferential wall of the column pipe. The sieve plate can be conveniently disassembled, the length of the inner tube extending into the column tube can be controlled during mounting, the distance between the sieve plate and the filler can be changed, dead volume caused by filler loss or chromatographic column collapse and the like can be compensated, and the conditions of reduction of separation degree and reduction of column efficiency of the chromatographic column are avoided.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, specifically to a liquid chromatography column. Background Technology

[0002] Liquid chromatography (LC) is a rapid preparative column chromatography technique. It relies on a pump to generate pressure, accelerating the elution rate of the mobile phase through a pre-packed chromatographic column. Its core component is the LC column, which typically consists of a column tube, pressure cap, ferrule, sieve plate, and connectors. Sieve plate clogging is a common problem encountered during LC column use, requiring removal for cleaning or replacement. Since the sieve plate is in contact with the packing material, some packing material is lost when removing it. Repeated cleaning of the sieve plate can lead to column collapse. Replacing the lost packing material to prevent collapse requires a high level of expertise, making column maintenance and cleaning difficult for users to perform themselves. Furthermore, column collapse is a frequent occurrence, leading to a significant increase in dead volume, poor peak shape, a marked decrease in resolution, and a significant reduction in column efficiency. Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a liquid chromatography column that allows for easy disassembly of the sieve plate and enables control of the length of the inner tube extending into the column tube during installation via a threaded sleeve that engages with the column tube thread. This alters the distance between the sieve plate and the packing material in the inner tube, ensuring close contact between the sieve plate and the packing material. This compensates for dead volume caused by packing loss or column collapse, preventing a decrease in resolution and column efficiency.

[0004] This utility model provides a liquid chromatography column to solve the above-mentioned technical problems, including a column tube filled with packing material. Both ends of the column tube have mounting heads. Each mounting head includes an inner tube inserted into the inner side of the column tube and an outer tube sleeved on the outer side of the column tube. The outer end of the inner tube is fixed to the outer tube by a connecting ring. A sealing ring is provided on the outer peripheral wall of the inner tube, and a limiting ring is provided on the inner end of the inner tube. A sieve plate is provided on the side of the limiting ring away from the column tube, and a clamping sleeve is provided on the side of the sieve plate away from the limiting ring. The outer peripheral wall of the clamping sleeve is threadedly connected to the inner peripheral wall of the inner tube. The clamping sleeve also has a liquid guiding hole coaxial with the column tube. An axial sliding guide is provided between the outer tube and the column tube. A threaded sleeve is rotatably connected to the end of the outer tube away from the connecting ring, and the threaded sleeve is threadedly connected to the outer peripheral wall of the column tube.

[0005] Furthermore, the sieve plate is provided with a limiting step, and the step surface of the limiting step abuts against the limiting ring.

[0006] Furthermore, a gasket is provided between the sieve plate and the clamping sleeve.

[0007] Furthermore, at least one annular mounting groove is provided on the outer peripheral wall of the inner tube, and the sealing ring is disposed in the annular mounting groove and partially contacts the inner peripheral wall of the column tube.

[0008] Furthermore, the axial sliding guide includes a guide protrusion provided on the inner wall of the outer tube and / or the outer wall of the column tube, and an axial guide groove provided on the outer wall of the column tube and / or the inner wall of the outer tube, wherein the axial guide groove slides in conjunction with the guide protrusion.

[0009] Furthermore, the clamping sleeve is provided with a buffer cavity coaxial with the liquid guiding hole on the side near the sieve plate, and the diameter of the buffer cavity is larger than the diameter of the liquid guiding hole.

[0010] Furthermore, a cap is provided on the extended end of the compression sleeve, and the inner peripheral wall of the cap is threadedly connected to the outer peripheral wall of the compression sleeve.

[0011] The beneficial effects of this utility model are as follows: The column tube is filled with packing material. The mounting heads at both ends of the column tube include an inner tube inserted into the inside of the column tube and an outer tube sleeved on the outside of the column tube. The extended end of the inner tube is fixed to the outer tube by a connecting ring, making the inner and outer tubes of the mounting head a whole. Movement of the outer tube can drive movement of the inner tube, which in turn drives movement of the sieve plate in the inner tube, changing the distance between the sieve plate and the packing material in the column tube, ensuring close contact and filling the dead volume in the column tube. An axial sliding guide is provided between the outer tube and the column tube, restricting the outer tube to move only along the axial direction of the column tube relative to it. This restricts the inner tube from moving axially within the column tube, preventing it from rotating inside the column tube and affecting the packing material. The outer tube, at the end furthest from the connecting ring, is rotatably connected to a threaded sleeve. This threaded sleeve is threadedly connected to the outer circumferential wall of the column tube. When the threaded sleeve is rotated, due to the circumferential limitation between the outer tube and the column tube, the threaded sleeve moves only axially along the column tube's outer circumferential wall, thus pulling the outer tube axially. This, in turn, drives the inner tube to move axially within the column tube, changing the distance between the sieve plate and the packing material pressed against the inner extension end of the inner tube. A limiting ring is provided on the inner extension end of the inner tube to limit the sieve plate. During installation, a clamping sleeve is used to press the sieve plate against the limiting ring to fix it. During installation, the sieve plate is placed into the inner tube, and then the clamping sleeve is threadedly connected to the inner tube, causing the clamping sleeve to move towards the limiting ring within the inner tube, pressing the sieve plate against the limiting ring. A liquid guide hole coaxial with the column tube is provided inside the clamping sleeve to facilitate material entry and exit from the inside of the column tube. A sealing ring on the outer circumferential wall of the inner tube is in close contact with the inner wall of the column tube, achieving a seal between the column tube and the inner tube.

[0012] When the screen plate needs to be disassembled, rotate the threaded sleeve to disengage it from the threads on the column tube. This will cause the outer and inner tubes to move simultaneously towards the open end of the column tube, ultimately removing the inner tube, outer tube, and clamping sleeve from the column tube. Then, rotate the clamping sleeve to disengage it from the inner tube, allowing the screen plate to be removed for cleaning or replacement. During installation, first place the screen plate into the inner tube, then rotate the clamping sleeve in the opposite direction to press the screen plate against the limiting ring of the inner tube. At this point, the screen plate, clamping sleeve, inner tube, and outer tube are all in place. The tubes are integrated as a whole. The inner tube is inserted into the opening of the column tube, and the outer tube is fitted onto the outside of the column tube. The threaded sleeve is rotated in the opposite direction to engage with the threads on the column tube, pulling the outer tube to the side of the column tube away from the opening end. This causes the inner tube to extend into the column tube. The length of the inner tube extending into the column tube can be controlled by the movement distance of the threaded sleeve, thereby controlling the distance between the sieve plate on the inner tube and the packing material. This ensures that the inner tube can make close contact with the packing material, compensate for the dead volume of the column tube, and avoid the decrease in resolution and column efficiency.

[0013] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 for Figure 1 A magnified view of the details of A.

[0016] In the attached diagram: 1-column tube, 11-axial guide groove, 2-mounting head, 21-inner tube, 211-limiting ring, 212-annular mounting groove, 22-outer tube, 221-guide protrusion, 23-connecting ring, 3-sealing ring, 4-sieve plate, 41-limiting step, 5-compression sleeve, 51-liquid guide hole, 52-buffer cavity, 6-threaded sleeve, 7-washer ring, 8-cap. Detailed Implementation

[0017] Referring to the accompanying drawings, the specific embodiments of this utility model will be described in detail.

[0018] Reference Figures 1 to 2 This utility model provides a liquid chromatography column, including a column tube 1 filled with packing material. Both ends of the column tube 1 are provided with mounting heads 2. The mounting head 2 includes an inner tube 21 inserted into the inner side of the column tube 1 and an outer tube 22 sleeved on the outer side of the column tube 1. The outer end of the inner tube 21 and the outer tube 22 are fixed together by a connecting ring 23, so that the inner tube 21 and the outer tube 22 of the mounting head 2 form a whole. The movement of the outer tube 22 can drive the movement of the inner tube 21, thereby driving the sieve plate 4 in the inner tube 21 to move, changing the distance between the sieve plate 4 and the packing material in the column tube 1, so that it is in close contact with the packing material and fills the dead volume in the column tube 1.

[0019] An axial sliding guide is provided between the outer tube 22 and the column tube 1, restricting the outer tube 22 to move only along the axial direction of the column tube 1. This restricts the inner tube 21 from moving axially within the column tube 1, preventing it from rotating inside the column tube 1 and affecting the packing. Furthermore, the axial sliding guide includes a guide protrusion 221 on the inner wall of the outer tube 22 and / or the outer wall of the column tube 1, and an axial guide groove 11 on the outer wall of the column tube 1 and / or the inner wall of the outer tube 22. The axial guide groove 11 slides in engagement with the guide protrusion 221. The guide protrusion 221 and the axial guide groove 11 cooperate to prevent rotation between the outer tube 22 and the column tube 1, so that it moves along the axial direction of the column tube 1. In this embodiment, the guide protrusion 221 is provided on the inner wall of the outer tube 22 and three sets are evenly distributed along the circumference of the inner wall of the outer tube 22. The axial guide groove 11 is provided on the outer wall of the column tube 1 and three sets are evenly distributed along the circumference of the outer wall of the column tube 1. The axial guide groove 11 is provided with an opening on the side close to the opening of the column tube 1, so that the guide protrusion 221 can be inserted into the axial guide groove 11 during installation.

[0020] The outer tube 22, away from the connecting ring 23, is rotatably connected to a threaded sleeve 6, which is threadedly connected to the outer peripheral wall of the column tube 1. Furthermore, the threaded sleeve 6 has a first internal thread on its inner side, and the outer peripheral wall of the column tube 1 has a first external thread that mates with the first internal thread. When the threaded sleeve 6 is rotated, due to the circumferential limitation between the outer tube 22 and the column tube 1, the threaded sleeve 6 moves only axially along the outer peripheral wall of the column tube 1, thereby pulling the outer tube 22 to move axially along the column tube 1. This, in turn, causes the inner tube 21 to move axially within the column tube 1 via the outer tube 22, changing the distance between the sieve plate 4 pressed against the inner end of the inner tube 21 and the packing material.

[0021] A sealing ring 3 is provided on the outer peripheral wall of the inner tube 21. The sealing ring 3 on the outer peripheral wall of the inner tube 21 is in close contact with the inner wall of the column tube 1, thereby sealing the column tube 1 and the inner tube 21. Further, at least one annular mounting groove 212 is provided on the outer peripheral wall of the inner tube 21. The sealing ring 3 is disposed within the annular mounting groove 212 and partially contacts the inner peripheral wall of the column tube 1. The annular mounting groove 212 limits the position of the sealing ring 3 on the outer peripheral wall of the inner tube 21. The sealing ring 3 is movably fitted with the inner wall of the column tube 1. When the inner tube 21 moves, the sealing ring 3 moves with the annular mounting groove 212, always remaining in contact with the inner wall of the column tube 1. In this embodiment, there are two annular mounting grooves 212, and correspondingly, there are two sealing rings 3.

[0022] The inner end of the inner tube 21 is provided with a limiting ring 211 to limit the sieve plate 4. The sieve plate 4 is provided on the side of the limiting ring 211 away from the column tube 1. The sieve plate 4 is provided with a clamping sleeve 5 on the side of the sieve plate 4 away from the limiting ring 211. The clamping sleeve 5 is used to press the sieve plate 4 onto the limiting ring 211 to fix the sieve plate 4. The outer peripheral wall of the clamping sleeve 5 is threadedly connected to the inner peripheral wall of the inner tube 21. The outer peripheral wall of the clamping sleeve 5 is provided with a second external thread, and the inner peripheral wall of the inner tube 21 is provided with a second internal thread that mates with the second external thread. The clamping sleeve 5 is also provided with a liquid guiding hole 51 coaxial with the column tube 1 to facilitate the entry and exit of materials from the inside of the column tube 1. Furthermore, the clamping sleeve 5 is provided with a buffer cavity 52 coaxial with the liquid guiding hole 51 on the side near the sieve plate 4. The diameter of the buffer cavity 52 is larger than the diameter of the liquid guiding hole 51, so that when the material enters the column tube 1 through the liquid guiding hole 51, it can be dispersed through the buffer cavity 52 and then quickly and fully diffused in the packing after passing through the sieve plate 4. During installation, the sieve plate 4 is placed in the inner tube 21, and then the clamping sleeve 5 is threaded to the inner tube 21, so that the clamping sleeve 5 moves in the direction of the limiting ring 211 in the inner tube 21 and presses the sieve plate 4 onto the limiting ring 211.

[0023] Furthermore, a cap 8 is provided on the extended end of the compression sleeve 5. The inner peripheral wall of the cap 8 is threadedly connected to the outer peripheral wall of the compression sleeve 5, and the inner peripheral wall of the cap 8 is provided with a third internal thread that mates with the second external thread. In this case, when the chromatographic column is not in use, the end of the chromatographic column can be sealed and protected by the cap 8.

[0024] Furthermore, the sieve plate 4 is provided with a limiting step 41, the step surface of which abuts against the limiting ring 211. At this time, the sieve plate 4 is limited by the abutment between the limiting step 41 and the limiting ring 211. Preferably, when the sieve plate 4 is pressed against the limiting ring 211, the inner end face of the sieve plate 4 is flush with the inner end face of the limiting step 41, ensuring tight contact between the sieve plate 4 and the packing. Furthermore, to reduce wear between the sieve plate 4 and the limiting ring 211 and to improve the sealing performance between them, a gasket 7 is provided between the sieve plate 4 and the pressing sleeve 5.

[0025] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0026] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A liquid chromatography column, characterized in that, It includes a column tube (1) filled with filler, and each end of the column tube (1) is provided with an installation head (2). The mounting head (2) includes an inner tube (21) inserted into the inside of the column tube (1) and an outer tube (22) sleeved on the outside of the column tube (1). The extended end of the inner tube (21) is fixed to the outer tube (22) by a connecting ring (23). A sealing ring (3) is provided on the outer peripheral wall of the inner tube (21), and a limiting ring (211) is provided on the inner extension end of the inner tube (21). A sieve plate (4) is provided on the side of the limiting ring (211) away from the column tube (1), and a clamping sleeve (5) is provided on the side of the sieve plate (4) away from the limiting ring (211). The outer peripheral wall of the clamping sleeve (5) is threadedly connected to the inner peripheral wall of the inner tube (21). A liquid guiding hole (51) coaxial with the column tube (1) is also provided inside the clamping sleeve (5). An axial sliding guide is provided between the outer tube (22) and the column tube (1). A threaded sleeve (6) is rotatably connected to one end of the outer tube (22) away from the connecting ring (23). The threaded sleeve (6) is threadedly connected to the outer peripheral wall of the column tube (1).

2. The liquid chromatography column according to claim 1, characterized in that, The sieve plate (4) is provided with a limiting step (41), and the step surface of the limiting step (41) abuts against the limiting ring (211).

3. The liquid chromatography column according to claim 1, characterized in that, A gasket (7) is provided between the sieve plate (4) and the clamping sleeve (5).

4. The liquid chromatography column according to claim 1, characterized in that, The outer peripheral wall of the inner tube (21) is provided with at least one annular mounting groove (212), and the sealing ring (3) is located in the annular mounting groove (212) and partially contacts the inner peripheral wall of the column tube (1).

5. The liquid chromatography column according to claim 1, characterized in that, The axial sliding guide includes a guide protrusion (221) provided on the inner wall of the outer tube (22) and / or the outer wall of the column tube (1), and an axial guide groove (11) provided on the outer wall of the column tube (1) and / or the inner wall of the outer tube (22), wherein the axial guide groove (11) slides in cooperation with the guide protrusion (221).

6. The liquid chromatography column according to claim 1, characterized in that, The clamping sleeve (5) is also provided with a buffer cavity (52) coaxial with the liquid guiding hole (51) on the side near the sieve plate (4), and the diameter of the buffer cavity (52) is larger than the diameter of the liquid guiding hole (51).

7. The liquid chromatography column according to claim 1, characterized in that, The outer end of the compression sleeve (5) is also provided with a cap (8), and the inner peripheral wall of the cap (8) is threadedly connected to the outer peripheral wall of the compression sleeve (5).

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