Spectrum detection and analysis device for canceration diagnosis

Abstract

The utility model relates to the field of tissue detection, in particular to a spectrum detection and analysis device for canceration diagnosis. The device comprises a laser generator, a spectrograph, a light transmission switching port, an objective lens and an objective table. Wherein the light-passing switching port, the objective lens and the objective table are sequentially arranged from top to bottom, the laser generator and the spectrograph are connected with the light-passing switching port through optical fibers, the objective table is used for placing a detection sample, and laser emitted by the laser generator passes through the objective lens and reaches the objective table to excite the detection sample to generate autofluorescence. And the objective lens collects the autofluorescence and transmits the autofluorescence to a spectrograph for signal conversion to obtain sample characteristic spectrum data. According to the utility model, the detection time is greatly shortened, a fresh detection sample can be used for detection, and the pretreatment step of the detection sample is reduced.

Description

Technical Field

[0001] This utility model relates to the field of tissue detection, and in particular to a spectral detection and analysis device for cancer diagnosis. Background Technology

[0002] Intraoperative pathological diagnosis is the core basis for determining the radical surgical plan for cancer. Currently, the routinely used "intraoperative frozen section-HE staining" pathological diagnosis mode takes an average of over half an hour. The procedure needs to be paused while waiting for the pathology report, extending the overall surgical time by 20%-40%. This not only significantly increases the risk of anesthetic complications for patients but also may lead to iatrogenic dissemination due to tumor exposure during the waiting period. Despite the continuous development of microscopic imaging technology in recent years, the traditional pathological diagnosis system still suffers from procedural delays and subjective diagnostic biases. Therefore, shortening the intraoperative pathological diagnosis time and increasing the theoretical support for pathological interpretation are crucial. Utility Model Content

[0003] The purpose of this invention is to overcome the above-mentioned shortcomings and provide a spectral detection and analysis device for cancer diagnosis, which has the advantages of short pathological diagnosis time and pathological analysis that does not rely on subjective experience.

[0004] This invention provides a spectral detection and analysis device for cancer diagnosis, comprising a laser generator, a spectrometer, a light exchange port, an objective lens, and a stage; wherein the light exchange port, objective lens, and stage are arranged sequentially from top to bottom, the laser generator and the spectrometer are both connected to the light exchange port, the stage is used to place the test sample, the laser generator emits a laser beam that passes through the objective lens to the stage to excite the test sample to generate autofluorescence, the objective lens collects the autofluorescence and transmits it to the spectrometer for signal conversion to obtain the characteristic spectral data of the sample.

[0005] Furthermore, the sample tested was lung tissue.

[0006] Furthermore, the device also includes an image forming element disposed above the light exchange port for collecting physical images of the test sample.

[0007] Furthermore, the device also includes a multi-axis calibration assembly, which includes, from bottom to top, an X-axis stage, a Y-axis stage, and a Z-axis stage. The Y-axis stage is connected to the moving end of the X-axis stage, and the moving ends of the two stages move in perpendicular directions. The Z-axis stage is connected to the free end of the Y-axis stage, and the moving ends of the two stages move in perpendicular directions. The stage is connected to the Z-axis stage.

[0008] Furthermore, the device also includes an exhaust fan located on one side of the laser generator.

[0009] Furthermore, the optical switching port is connected to the laser generator via optical fiber.

[0010] Furthermore, the optical switching port is connected to the spectrometer via optical fiber.

[0011] By adopting the above technical solution, the beneficial effects of this utility model are:

[0012] This invention utilizes the autofluorescence characteristic of test samples under laser excitation. An objective lens connected to a laser generator focuses the laser onto the test sample on the stage. Simultaneously, the objective lens collects the autofluorescence of the test sample and transmits it to a spectrometer to generate spectral data. This spectral data reveals the differences in fluorescence characteristics between tumor tissues and their subtypes and normal tissues, increasing data support for pathological diagnosis and reducing reliance on subjective judgment. Furthermore, this invention supports the testing of fresh test samples, reducing pretreatment steps and enabling rapid intraoperative pathological diagnosis.

[0013] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit this disclosure.

[0014] Undoubtedly, such and other objects of this invention will become more apparent after the following detailed description of the preferred embodiments, which are illustrated in various accompanying drawings and figures.

[0015] To make the above and other objects, features and advantages of this utility model more apparent and understandable, one or more preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0016] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0017] In the accompanying drawings, the same parts use the same reference numerals, and the drawings are schematic and not necessarily drawn to actual scale.

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only one or more embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on such drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the internal structure of a spectral detection and analysis device for cancer diagnosis according to this utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of a spectral detection and analysis device for cancer diagnosis according to this utility model from another perspective.

[0021] Figure 3 This is another schematic diagram of the internal structure of the spectral detection and analysis device for cancer diagnosis according to this utility model.

[0022] Figure 4 This is a schematic diagram of the multi-axis calibration component of a spectral detection and analysis device for cancer diagnosis according to this utility model;

[0023] Explanation of key figure labels:

[0024] 1. Laser generator;

[0025] 21. Optical switching port;

[0026] 22. Objective lens;

[0027] 23. Stage;

[0028] 24. Image-molded parts;

[0029] 3. Spectrometer;

[0030] 4. Multi-axis calibration assembly;

[0031] 41. X-axis running table;

[0032] 42. Y-axis running table;

[0033] 43. Z-axis running table;

[0034] 5. Exhaust fan. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present utility model and are not intended to limit the present utility model.

[0036] Reference Figure 1-4This utility model provides a laser generator 1, a spectrometer 3, a light exchange port 21, an objective lens 22, and a stage 23; wherein the light exchange port 21, the objective lens 22, and the stage 23 are arranged sequentially from top to bottom. The light exchange port 21 is used to introduce laser light and export autofluorescence, so two adapters can be provided on the light exchange port 21, and the two adapters are connected to the laser generator 1 and the spectrometer 3 respectively via optical fibers; the light exchange port 21 can also be provided with one adapter, through which a Y-shaped light ray is connected at one end to the adapter, and the other two ends are connected to the laser generator 1 and the spectrometer 3 respectively. The stage 23 is used to place the test sample. In order to achieve focusing of the objective lens, the stage 23 is equipped with a position adjustment function. Therefore, the device also includes a multi-axis calibration assembly 4. The multi-axis calibration assembly 4 includes, from bottom to top, an X-axis stage 41, a Y-axis stage 42, and a Z-axis stage 43. The Y-axis stage 42 is connected to the moving end of the X-axis stage 41 and the moving ends of the two are perpendicular to each other. The Z-axis stage 43 is connected to the free end of the Y-axis stage 42 and the moving ends of the two are perpendicular to each other. The stage 23 is connected to the Z-axis stage 43, and the corresponding stage is controlled to achieve position adjustment of the stage 23.

[0037] Laser generator 1 emits a 405nm laser, which passes through objective lens 22 to stage 23 to excite the sample to produce autofluorescence. At this time, the wavelength of autofluorescence is greater than 405nm. Objective lens 22 collects the autofluorescence and transmits it to spectrometer 3 for signal conversion and finally inputs it into computer to obtain sample characteristic spectral data, i.e. fluorescence spectrum. The sample to be detected can be lung tissue.

[0038] Furthermore, to achieve real-time image acquisition of the test sample and thus monitor the focusing status of the objective lens 22, the device also includes an image forming component 24, specifically a camera. The image forming component 24 is located above the light exchange port 21 to collect physical images of the test sample. The image forming component 24 can achieve automatic focusing through external computer software. Specifically, the image forming component 24 captures the laser light illuminating the test assembly, and uses the captured laser light intensity and spot size data to adjust the position of the multi-axis calibration component, thereby achieving automatic focusing of the device. After the above equipment is assembled, a housing is required. For overall heat dissipation, the device also includes an exhaust fan 5, which is located on one side of the laser generator 1.

[0039] Working principle: When in use, a sample slice is placed on a glass slide, and then the glass slide is placed on the stage 23. The laser generator 1 is activated to emit a 405nm laser to reach the objective lens 22 and excite the sample to generate autofluorescence. The generated autofluorescence is collected by the objective lens 22 and input into the spectrometer 3 for conversion to obtain fluorescence data. During this process, the position of the multi-axis calibration component 4 is adjusted according to the image of the image forming component 24.

[0040] It should be understood that the embodiments disclosed herein are not limited to the specific processing steps or materials disclosed herein, but should be extended to equivalent substitutions of such features as understood by those skilled in the art. It should also be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting.

[0041] The term "embodiment" in this specification refers to a specific feature or characteristic described in connection with an embodiment that is included in at least one embodiment of the present invention. Therefore, phrases or "embodiments" appearing in various places throughout the specification do not necessarily refer to the same embodiment.

[0042] Furthermore, the described features or characteristics may be incorporated into one or more embodiments in any other suitable manner. In the above description, specific details, such as thickness, quantity, etc., are provided to provide a comprehensive understanding of embodiments of the present invention. However, those skilled in the art will understand that the present invention can be implemented without the aforementioned one or more specific details or may be implemented using other methods, components, materials, etc.

Claims

1. A spectral detection and analysis device for cancer diagnosis, characterized in that, The device includes a laser generator, a spectrometer, a light exchange port, an objective lens, and a stage. The light exchange port, objective lens, and stage are arranged sequentially from top to bottom. The laser generator and the spectrometer are both connected to the light exchange port. The stage is used to place the sample for testing. The laser generator emits a laser beam that passes through the objective lens to the stage, exciting the sample to generate autofluorescence. The objective lens collects the autofluorescence and transmits it to the spectrometer for signal conversion to obtain the sample's characteristic spectral data.

2. The spectral detection and analysis device for cancer diagnosis according to claim 1, characterized in that, The sample tested was lung tissue.

3. The spectral detection and analysis device for cancer diagnosis according to claim 1, characterized in that, It also includes an image forming component, which is located above the light exchange port for collecting physical images of the test sample.

4. The spectral detection and analysis device for cancer diagnosis according to claim 1, characterized in that, It also includes a multi-axis calibration assembly, which from bottom to top includes an X-axis stage, a Y-axis stage, and a Z-axis stage. The Y-axis stage is connected to the moving end of the X-axis stage and the moving ends of the two stages move in perpendicular directions. The Z-axis stage is connected to the free end of the Y-axis stage and the moving ends of the two stages move in perpendicular directions. The stage is connected to the Z-axis stage.

5. The spectral detection and analysis device for cancer diagnosis according to claim 1, characterized in that, It also includes an exhaust fan located on one side of the laser generator.

6. The spectral detection and analysis device for cancer diagnosis according to claim 1, characterized in that, The optical switch is connected to the laser generator via optical fiber.

7. The spectroscopic detection and analysis apparatus for cancer diagnosis according to any one of claims 1 or 6, characterized in that, The optical switch port is connected to the spectrometer via optical fiber.

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