Double-column circulating chromatographic system

Abstract

A double-column circulating chromatographic system comprises two valve banks and two chromatographic columns, and the two valve banks comprise the first valve bank and the second valve bank; the first valve group consists of first to fourth control valves; the second valve group consists of fifth to eighth control valves; the first valve group and the second valve group are each internally provided with four multi-channel connectors, and the four multi-channel connectors in each valve group are sequentially connected with the four control valves in the corresponding valve group. The two chromatographic columns comprise a first chromatographic column and a second chromatographic column; inlets of the first chromatographic column and the second chromatographic column are respectively connected with the multi-channel joints on the corresponding sides in the first valve group through pipelines; outlets of the first chromatographic column and the second chromatographic column are respectively connected with the multi-channel joints on the corresponding sides in the second valve group through pipelines; pipelines which can be connected with each other are arranged between the first valve group and the second valve group; one multi-channel joint in the first valve group is used for introducing a raw material solution and / or an eluent; one multi-channel joint in the second valve group is used for outflow of waste liquid or product distillate; the device has the advantage that residues in the connecting pipeline between the two columns can be eliminated.

Description

Technical Field

[0001] This utility model relates to the field of chromatography system technology, specifically to a dual-column circulating chromatography system. Background Technology

[0002] Circulating chromatography indirectly extends the chromatographic column, improving separation without increasing column pressure. Patent ZL202420443955.3 discloses a dual-column circulating chromatography system based on a two-position four-way valve, which has four states (…). Figure 1-4 As shown, its structure includes a first chromatographic column 1, a second chromatographic column 2, a first four-way valve group 3 and a second four-way valve group 4, and connecting pipelines 5 connecting various components within the system. When the system switches back and forth between the first and second states, a dual-column cyclic chromatographic separation process is realized, while in the third or fourth state, strongly retained impurities inside the first chromatographic column 1 or the second chromatographic column 2 can be flushed separately.

[0003] Although the dual-column chromatography system disclosed in ZL202420443955.3 has a reasonable structure and simple pipeline connections, there is still a problem. For example, when the target analyte concentration band elutes from the first column 1 to the second column 2 in the nth switching cycle, there will inevitably be residual impurities (impurities that elute later than the target component peak, strongly retained components) in the connecting line 5 between the two columns. Then, in the (n+1)th switching cycle, the target analyte band elutes from the second column 2 back to the first column 1 through the same line. At this time, the residual impurities in the connecting line 5 between the two columns come into contact with and mix with the leading edge of the target analyte band, thus contaminating the target component. Therefore, when the impurity limit requirements are very strict, this pipeline impurity residue problem may become a key factor affecting product quality. Utility Model Content

[0004] This application addresses the aforementioned shortcomings of the prior art by providing a dual-column circulating chromatography system capable of eliminating residues inside the connecting tubing between the two columns.

[0005] To solve the above-mentioned technical problems, the technical solution adopted in this application is as follows: a dual-column circulating chromatography system, the structure of which includes two valve groups and two chromatographic columns; the two valve groups include a first valve group and a second valve group; the first valve group consists of a first control valve, a second control valve, a third control valve, and a fourth control valve; the second valve group consists of a fifth control valve, a sixth control valve, a seventh control valve, and an eighth control valve; both the first and second valve groups contain four multi-channel connectors, and the four multi-channel connectors in each valve group are sequentially connected to the four control valves in the corresponding valve group (forming a loop, with a distance between every two multi-channel connectors). The system includes a control valve (with a multi-channel connector between every two control valves); two chromatographic columns, including a first column and a second column; the inlets of the first and second columns are connected via pipelines to the corresponding multi-channel connectors of the first valve group; the outlets of the first and second columns are connected via pipelines to the corresponding multi-channel connectors of the second valve group; a pipeline connecting the first and second valve groups is provided; one multi-channel connector in the first valve group is used for the introduction of raw material and / or eluent; and one multi-channel connector in the second valve group is used for the outflow of waste liquid or product distillate.

[0006] By adopting the above structure, this application enables the dual-column circulating chromatography system to not only maintain the first and second chromatographic columns in series, but also to disconnect the two columns to flush each column separately. More importantly, this structure can also flush the connecting pipeline between the two columns, thereby cleaning up any residual impurities in the connecting pipeline and preventing them from contacting and mixing with the front edge of the target analyte band, thus contaminating the target component.

[0007] Furthermore, the first valve group contains four multi-channel connectors, namely a first multi-channel connector, a second multi-channel connector, a third multi-channel connector, and a fourth multi-channel connector, which are arranged circumferentially in a clockwise direction within the first valve group. The second valve group contains four multi-channel connectors, namely a fifth multi-channel connector, a sixth multi-channel connector, a seventh multi-channel connector, and an eighth multi-channel connector, which are arranged circumferentially in a clockwise direction within the second valve group. With this structural arrangement, the multi-channel connectors and control valves in each valve group are interconnected to form a closed-loop structure, with a control valve between every two multi-channel connectors and a multi-channel connector between every two control valves. This structure can effectively control the flow direction of fluid within the system, reduce the probability of mixing of downstream impurities and target components, and thus avoid contaminating the target components.

[0008] Furthermore, the inlets of the first and second chromatographic columns are connected to the second and fourth multi-channel connectors of the first valve group via pipelines, respectively; the outlets of the first and second chromatographic columns are connected to the sixth and eighth multi-channel connectors of the second valve group via pipelines, respectively. With the above structure, the two chromatographic columns can be effectively adjusted to be connected in series or disconnected according to the separation needs of the system.

[0009] Furthermore, the first valve group and the second valve group are connected by a pipeline, one end of which is connected to the third multi-channel connector of the first valve group and the other end of which is connected to the fifth multi-channel connector of the second valve group. In this way, the series connection and disconnection of the two chromatographic columns can be flexibly adjusted according to the separation needs. More importantly, it can also remove residual impurities in the connecting pipeline, thereby preventing residual impurities in the pipeline from contacting and mixing with the front edge of the target analyte band and contaminating the target component.

[0010] Furthermore, the first multi-channel connector of the first valve group is used for the introduction of raw material liquid and / or eluent; the seventh multi-channel connector of the second valve group is used for the outflow of waste liquid or product distillate; with this structure, the entry and exit positions of fluids can be effectively controlled.

[0011] Furthermore, the first, third, fifth, and seventh control valves are opened, while the second, fourth, sixth, and eighth control valves are closed, and the fluid in the system flows sequentially through the first and second chromatographic columns. With this scheme, the first chromatographic column can be used as the upstream column, realizing the series connection function of the two chromatographic columns.

[0012] Furthermore, the first, third, fifth, and seventh control valves are closed, while the second, fourth, sixth, and eighth control valves are opened, allowing the fluid in the system to flow sequentially through the second chromatographic column and the first chromatographic column. Using this scheme, the second chromatographic column can be used as the upstream column, realizing the tandem function of the two chromatographic columns.

[0013] Furthermore, the first and sixth control valves are opened, while the second, third, fourth, fifth, seventh, and eighth control valves are closed, so that the fluid in the system flows only through the first chromatographic column. By adopting this scheme, the first and second chromatographic columns can be completely disconnected, so as to achieve the purpose of rinsing the first chromatographic column separately.

[0014] Furthermore, the fourth and seventh control valves are opened, while the first, second, third, fifth, sixth, and eighth control valves are closed, so that the fluid in the system flows only through the second chromatographic column. By adopting this scheme, the first and second chromatographic columns can be completely disconnected, so as to achieve the purpose of rinsing the second chromatographic column separately.

[0015] Furthermore, the third, fourth, fifth, and sixth control valves are closed, while the first, second, seventh, and eighth control valves are opened, allowing the fluid in the system to flow through the connecting pipeline between the first and second chromatographic columns. This method allows for the flushing of the connecting pipeline between the two columns, thereby cleaning away any residual impurities within the pipeline and preventing them from contacting and mixing with the leading edge of the target analyte band, thus contaminating the target component.

[0016] Furthermore, the third, fourth, fifth, and sixth control valves are opened, while the first, second, seventh, and eighth control valves are closed, allowing the fluid in the system to flow through the connecting pipeline between the first and second chromatographic columns. This method allows for the flushing of the connecting pipeline between the two columns, thereby cleaning away any residual impurities within the pipeline and preventing them from contacting and mixing with the target analyte's spectral front, thus contaminating the target component.

[0017] Furthermore, the first, fourth, sixth, and seventh control valves are opened, while the second, third, fifth, and eighth control valves are closed, and the fluid in the system flows through the first and second chromatographic columns respectively; with this scheme, the first and second chromatographic columns are connected in parallel, and the fluid does not pass through the connecting pipeline between the two chromatographic columns.

[0018] Furthermore, the multi-channel connector is a structure with at least three channels; this structure can meet the connection requirements of each multi-channel connector at the corresponding position, and realize the adjustment requirements for different states of the system.

[0019] Furthermore, detectors are installed on the outlet lines of the first and second chromatographic columns; the detectors can monitor the chromatographic signals and provide chromatograms; the detectors specifically include one or more of the following: ultraviolet detectors, evaporative light detectors, and differential refractive index detectors. Attached image description:

[0020] Figure 1 This is a schematic diagram of the structure of a dual-column circulating chromatography system using a two-position four-way valve in the first state, according to existing technology.

[0021] Figure 2 This is a schematic diagram of the structure of a dual-column circulating chromatography system using a two-position four-way valve in the second state, according to existing technology.

[0022] Figure 3 This is a schematic diagram of the structure of a dual-column circulating chromatography system using a two-position four-way valve in the third state, according to existing technology.

[0023] Figure 4 This is a schematic diagram of the structure of a dual-column circulating chromatography system using a two-position four-way valve in the fourth state, according to existing technology.

[0024] As shown in the attached diagram: 1. First chromatographic column, 2. Second chromatographic column, 3. First four-way valve assembly, 4. Second four-way valve assembly, 5. Connecting pipeline.

[0025] Figure 5 This is a schematic diagram of the dual-column circulating chromatography system of this application in its first state.

[0026] Figure 6 This is a schematic diagram of the dual-column circulating chromatography system of this application in its second state.

[0027] Figure 7 This is a schematic diagram of the dual-column circulating chromatography system of this application in its third state.

[0028] Figure 8 This is a schematic diagram of the dual-column circulating chromatography system of this application in its fourth state.

[0029] Figure 9 This is a schematic diagram of the dual-column circulating chromatography system of this application in its fifth state.

[0030] Figure 10 This is a schematic diagram of the dual-column circulating chromatography system of this application in its sixth state.

[0031] Figure 11 This is a schematic diagram of the dual-column circulating chromatography system of this application in its seventh state.

[0032] As shown in the attached diagram: S1. First valve group, v1. First control valve, v2. Second control valve, v3. Third control valve, v4. Fourth control valve, S2. Second valve group, v5. Fifth control valve, v6. Sixth control valve, v7. Seventh control valve, v8. Eighth control valve, 110. First multi-channel connector, 120. Second multi-channel connector, 130. Third multi-channel connector, 140. Fourth multi-channel connector, 210. Fifth multi-channel connector, 220. Sixth multi-channel connector, 230. Seventh multi-channel connector, 240. Eighth multi-channel connector, C1. First chromatographic column, C2. Second chromatographic column, D1. First detector, D2. Second detector, 1. Piping, 2. Feed pump, 3. Eluent pump. Detailed Implementation

[0033] The technical solution of this utility model will be further described below with reference to the accompanying drawings.

[0034] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments and accompanying drawings. Obviously, the described embodiments are merely preferred embodiments, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this invention.

[0035] As attached Figure 5-11 The diagram illustrates a dual-column circulating chromatography system according to this application. The system comprises two valve groups and two chromatographic columns. The two valve groups include a first valve group S1 and a second valve group S2. The first valve group S1 consists of a first control valve v1, a second control valve v2, a third control valve v3, and a fourth control valve v4. The second valve group S2 consists of a fifth control valve v5, a sixth control valve v6, a seventh control valve v7, and an eighth control valve v8. Both the first valve group S1 and the second valve group S2 contain four multi-channel connectors. Each valve group contains four multi-channel connectors. The multi-channel connector is sequentially connected to the four control valves in the corresponding valve group (forming a loop, with a control valve between every two multi-channel connectors and a multi-channel connector between every two control valves); the two chromatographic columns include a first chromatographic column C1 and a second chromatographic column C2; the inlets of the first chromatographic column C1 and the second chromatographic column C2 are respectively connected to the multi-channel connectors on the corresponding side of the first valve group S1 via pipeline 1; the outlets of the first chromatographic column C1 and the second chromatographic column C2 are respectively connected to the multi-channel connectors on the corresponding side of the second valve group S2 via pipelines; the first valve... A pipeline 1 is provided between valve group S1 and valve group S2, allowing them to be interconnected. A multi-channel connector in valve group S1 is used for the introduction of feed liquid (which can be pumped into the system by feed liquid pump 2) or eluent (which can be pumped into the system by eluent pump 3). A multi-channel connector in valve group S2 is used for the outflow of waste liquid (impurities, pre-impurities, or post-impurities, etc.) or product (target component) distillate. Specifically, both valve groups in this application include at least four control valves and four multi-channel connectors. A multi-channel connector and four control valves are alternately connected (i.e., each multi-channel connector has two ports connected to one control valve and the other port connected to the corresponding side of the chromatographic column, raw material, eluent inlet, detector, or waste liquid or product outlet; each control valve has two ports connected to one multi-channel connector), forming a complete valve group. The flow of fluids in the system along different routes is achieved by opening and closing the control valves. The control valves in this application can be any valve structure commonly used in chromatographic systems that can control the opening and closing of pipelines.

[0036] By adopting the above structure, this application, through the setting of multi-channel connector structure, control valve and connection sequence, enables the constructed dual-column circulating chromatography system to not only keep the first and second chromatographic columns in series at all times, but also to disconnect the two chromatographic columns to flush each column separately; more importantly, this structure of this application can also flush the connecting pipeline between the two chromatographic columns, thereby cleaning up the residual impurities in the connecting pipeline and preventing the residual impurities in the pipeline from contacting and mixing with the front edge of the target analyte band and contaminating the target component.

[0037] As attached Figure 5-11 As shown, the first valve group S1 of this application contains four multi-channel connectors: a first multi-channel connector 110, a second multi-channel connector 120, a third multi-channel connector 130, and a fourth multi-channel connector 140. These first to fourth multi-channel connectors are arranged clockwise in a circumferential manner within the first valve group S1 (with a control valve between each multi-channel connector). The second valve group S2 contains four multi-channel connectors: a fifth multi-channel connector 210, a sixth multi-channel connector 220, a seventh multi-channel connector 230, and an eighth multi-channel connector 240. These fifth to eighth multi-channel connectors are arranged clockwise in a circumferential manner within the second valve group S2 (with a control valve between each multi-channel connector). This structural arrangement interconnects the multi-channel connectors and control valves within each valve group to form a closed-loop structure. There is a control valve between every two multi-channel connectors, and a multi-channel connector between every two control valves. This structure enables effective control of the fluid flow direction within the system, reducing the probability of mixing with downstream impurities and the target component, thereby preventing contamination of the target component.

[0038] As attached Figure 5-11 As shown, the inlets of the first chromatographic column C1 and the second chromatographic column C2 (the left side of the diagram indicates the column inlet direction) are connected to the second multi-channel connector 120 and the fourth multi-channel connector 140 of the first valve group S1 via pipeline 1, respectively; the outlets of the first chromatographic column C1 and the second chromatographic column C2 (the right side of the diagram indicates the column outlet direction) are connected to the sixth multi-channel connector 220 and the eighth multi-channel connector 240 of the second valve group S2 via pipeline 1, respectively; with the above structure, the two chromatographic columns can be effectively adjusted to be connected in series or disconnected according to the separation needs of the system.

[0039] As attached Figure 5-11As shown, a pipeline 1 is provided between the first valve group S1 and the second valve group S2 described in this application. One end of the pipeline is connected to the third multi-channel connector 130 of the first valve group S1, and the other end is connected to the fifth multi-channel connector 210 of the second valve group S2. In this way, the series connection and disconnection of the two chromatographic columns can be flexibly adjusted according to the separation needs. More importantly, it can also remove the residual impurities in the connecting pipeline, thereby avoiding the residual impurities in the pipeline from contacting and mixing with the front edge of the target analyte band and contaminating the target component.

[0040] As attached Figure 5-11 As shown, the first multi-channel connector 110 of the first valve group S1 described in this application is used for the introduction of raw material liquid and / or eluent (specifically, the raw material liquid can be introduced into the system through the raw material liquid pump 2, and the eluent can be introduced into the system through the eluent pump 3); the seventh multi-channel connector 230 of the second valve group S2 is used for the outflow of waste liquid (impurities in the substance to be separated and purified, such as downstream or upstream impurities) or product distillate (target components in the substance to be separated and purified); with this structure, the entry and exit positions of fluids can be effectively controlled.

[0041] Based on the above-described dual-column circulating chromatography system structure, the system of this application can at least present the separation and purification states of the following embodiments: It should be noted that when a certain control valve and the corresponding pipeline are closed and no fluid enters, it is represented by a dashed line in the attached drawings, while the corresponding solid line route and control valve represent the fluid flow in that pipeline, realizing the operation of the material to be separated and purified.

[0042] Example 1

[0043] As attached Figure 5 As shown, in the dual-column circulating chromatography system of this application, the first, third, fifth, and seventh control valves are opened, while the second, fourth, sixth, and eighth control valves are closed. The fluid in the system flows sequentially through the first chromatographic column C1 and the second chromatographic column C2. Using this scheme, the corresponding state and appendix of this embodiment... Figure 1 The states shown are consistent, which can realize the function of the first chromatographic column as the upstream column and realize the series connection of the two chromatographic columns.

[0044] Example 2

[0045] As attached Figure 6 As shown, in this application, the first, third, fifth, and seventh control valves are closed, while the second, fourth, sixth, and eighth control valves are opened, and the fluid in the system flows sequentially through the second chromatographic column C2 and the first chromatographic column C1; using this scheme, the corresponding state and appendix of this embodiment are as follows. Figure 2 The states shown are consistent, which allows the second chromatographic column to be used as the upstream column, realizing the function of connecting the two chromatographic columns in series.

[0046] Example 3

[0047] As attached Figure 7 As shown, this application opens the first and sixth control valves while closing the second, third, fourth, fifth, seventh, and eighth control valves. The fluid in the system flows only through the first chromatographic column C1 and is then directly discharged from the port of a multi-channel connector connected to the outlet side of the first chromatographic column C1. This scheme allows for complete disconnection of the first chromatographic column C1 and the second chromatographic column C2, achieving the purpose of rinsing the first chromatographic column C1 separately, and is consistent with existing technologies. Figure 3 The states shown are consistent.

[0048] Example 4

[0049] As attached Figure 8 As shown, this application opens the fourth and seventh control valves in the system while closing the first, second, third, fifth, sixth, and eighth control valves. The fluid in the system flows only through the second chromatographic column C2 and then directly discharges from one port of the multi-channel connector connected to the outlet side of the second chromatographic column C2. Using this scheme, the first chromatographic column C1 and the second chromatographic column C2 can be completely disconnected, achieving the purpose of rinsing the second chromatographic column C2 separately. Its state is similar to that of existing technologies. Figure 4 The states shown are consistent.

[0050] Example 5

[0051] As attached Figure 9 As shown, this application closes the third, fourth, fifth, and sixth control valves in the system, while opening the first, second, seventh, and eighth control valves. The fluid in the system flows directly through the connecting line 1 between the first chromatographic column C1 and the second chromatographic column C2, while no fluid flows inside the first and second chromatographic columns. By adopting this scheme, the connecting line between the two chromatographic columns can be flushed, thereby cleaning up any residual impurities in the connecting line and preventing residual impurities from contacting and mixing with the front edge of the target analyte band, thus contaminating the target component.

[0052] Example 6

[0053] As attached Figure 10 As shown, this application opens the third, fourth, fifth, and sixth control valves in the system, while closing the first, second, seventh, and eighth control valves. The fluid in the system flows directly through the connecting line 1 between the first chromatographic column C1 and the second chromatographic column C2, while no fluid flows inside the first and second chromatographic columns. Using this scheme, the connecting line between the two chromatographic columns can be flushed, thereby cleaning away any residual impurities in the connecting line and preventing them from contacting and mixing with the front edge of the target analyte band, thus contaminating the target component.

[0054] Example 7

[0055] As attached Figure 11 As shown, this application opens the first, fourth, sixth, and seventh control valves in the system, while closing the second, third, fifth, and eighth control valves. The fluid in the system flows through the first chromatographic column C1 and the second chromatographic column C2, respectively. In this scheme, the first and second chromatographic columns are connected in parallel, and the fluid does not pass through the connecting line 1 between the two chromatographic columns, but only through the two chromatographic columns, and finally merges and flows out of the system.

[0056] As an example, the multi-channel connector described in this application is a connector structure with at least three channels (a connector structure with three ports), specifically in... Figure 5-11 The diagram shows a three-channel connector structure. This structure can meet the connection requirements of each multi-channel connector in its corresponding position, and realize the adjustment needs of different system states.

[0057] As attached Figure 5-11 As shown, detectors are installed on the outlet lines of the first chromatographic column C1 and the second chromatographic column C2, specifically the detector D1 installed on the outlet line of the first chromatographic column C1 and the detector D2 installed on the outlet line of the second chromatographic column C2. The chromatographic signal can be monitored and a chromatogram can be generated by the detectors. The detectors specifically include one or more of the following: ultraviolet detector, evaporative light detector, and differential refractive index detector.

[0058] The system described in this application can switch between different states corresponding to at least seven embodiments, and the state of the system can be flexibly adjusted according to the needs of separating and purifying substances.

[0059] (1) As the system of this application is in Figure 5 The first state shown (Example 1) and Figure 6 When switching back and forth between the second state shown (Example 2), the upstream and downstream positions of the first chromatographic column C1 and the second chromatographic column C2 are changed repeatedly, thereby indirectly extending the column length; at a certain switching time or switching cycle, when the system is in Figure 5 In the first state shown (Example 1), after the target component flows from the first chromatographic column C1 to the second chromatographic column C2, strongly retained impurities remain in the first chromatographic column C1. At this time, the system can be switched to the second chromatographic column. Figure 7 The third state shown (Example 3) can be used to wash away strongly retained impurities remaining in the first chromatographic column C1 separately;

[0060] (2) For example, at a certain switching time or switching cycle, when the system is in a state of flux. Figure 6In the second state shown (Example 2), after the target component flows from the second column C2 to the first column C1, strongly retained impurities remain in the second column C2. At this time, the system can be switched to the attached... Figure 8 The fourth state shown (Example 4) allows for the separate rinsing of strongly retained impurities remaining in the second chromatographic column C2;

[0061] (3) For example, at a certain switching time or switching cycle, when the system is in Figure 5 In the first state shown (Example 1), after the target component flows from the first chromatographic column C1 to the second chromatographic column C2, the connecting line 1 between the outlet of the first chromatographic column C1 and the inlet of the second chromatographic column C2 will retain strong impurities. At this time, the system can be switched to Figure 9 The fifth state shown (Example 5) allows the strong impurities remaining in this section of the connecting pipeline 1 to be flushed out of the system.

[0062] (4) For example, at a certain switching time or switching cycle, when the system is in such a state Figure 6 In the second state shown (Example 2), after the target component flows from the second column C2 to the first column C1, the connecting line 1 between the outlet of the second column C2 and the inlet of the first column C1 will retain strong impurities. At this time, the system can be switched to... Figure 10 The sixth state shown (Example 10) allows the strong impurities remaining in this section of the connecting pipeline 1 to be flushed out of the system.

[0063] (5) At a certain switching time or switching cycle, the system can be switched to a state such as Figure 11 As shown, when the two chromatographic columns are connected in parallel, this parallel connection not only improves the separation efficiency of the system, but also makes the system more flexible and accurate when processing complex samples.

[0064] As can be seen from the above embodiments and technical solutions, the technical solutions of this application can achieve the following technical effects:

[0065] 1. The dual-column cyclic chromatography structure involved in the system of this application can switch back and forth between the seven states shown in at least Examples 1-7 above, which can accurately control the eluent at multiple positions in the system based on the prior art, achieve more precise and efficient operation, and thus effectively improve separation efficiency;

[0066] 2. The core components of this utility model include two valve assemblies consisting of four independently controlled valves and four multi-channel connector structures within each valve assembly. A complete system structure is formed by controlling the valves and valve assemblies in a specific connection sequence. This solution can conveniently achieve the four conventional states (…). Figure 5-8 As shown, compared with existing technology Figure 1-The status shown is consistent), that is, switching between the upstream and downstream positions of the chromatographic column and flushing the two columns separately. Importantly, the connecting pipeline between the upstream column outlet and the downstream column inlet can also be flushed. Figure 9-10 (as shown) and the ability to use two chromatographic columns in parallel (as shown) Figure 11 (as shown); especially Figure 9-10 The state shown allows for direct flushing of the connecting lines between two chromatographic columns or between two valve groups, thereby removing residual impurities from the lines and preventing them from contacting and mixing with the target analyte band front, thus effectively overcoming the shortcomings of existing technologies.

Claims

1. A dual-column circulating chromatography system, the system comprising two valve assemblies and two chromatographic columns, characterized in that: The two valve groups include a first valve group and a second valve group; the first valve group consists of a first control valve, a second control valve, a third control valve, and a fourth control valve; the second valve group consists of a fifth control valve, a sixth control valve, a seventh control valve, and an eighth control valve; both the first and second valve groups contain four multi-channel connectors, and the four multi-channel connectors in each valve group are sequentially connected to the four control valves in the corresponding valve group; the two chromatographic columns include a first chromatographic column and a second chromatographic column; the inlets of the first and second chromatographic columns are respectively connected to the multi-channel connectors on the corresponding sides of the first valve group via pipelines; the outlets of the first and second chromatographic columns are respectively connected to the multi-channel connectors on the corresponding sides of the second valve group via pipelines; the first and second valve groups are connected by pipelines; one multi-channel connector in the first valve group is used for the introduction of raw material liquid and / or eluent; one multi-channel connector in the second valve group is used for the outflow of waste liquid or product distillate.

2. The dual-column circulating chromatography system according to claim 1, characterized in that: The first valve group contains four multi-channel connectors, namely a first multi-channel connector, a second multi-channel connector, a third multi-channel connector, and a fourth multi-channel connector, and the first to fourth multi-channel connectors are arranged circumferentially in a clockwise direction within the first valve group; the second valve group contains four multi-channel connectors, namely a fifth multi-channel connector, a sixth multi-channel connector, a seventh multi-channel connector, and an eighth multi-channel connector, and the fifth to eighth multi-channel connectors are arranged circumferentially in a clockwise direction within the second valve group.

3. The dual-column circulating chromatography system according to claim 2, characterized in that: The inlets of the first and second chromatographic columns are connected to the second and fourth multi-channel connectors of the first valve group via pipelines, respectively; the outlets of the first and second chromatographic columns are connected to the sixth and eighth multi-channel connectors of the second valve group via pipelines, respectively.

4. The dual-column circulating chromatography system according to claim 3, characterized in that: The first valve group and the second valve group are connected by a pipeline, one end of which is connected to the third multi-channel connector of the first valve group and the other end of which is connected to the fifth multi-channel connector of the second valve group.

5. The dual-column circulating chromatography system according to claim 4, characterized in that: The first multi-channel connector of the first valve group is used for the introduction of raw material liquid and / or eluent; the seventh multi-channel connector of the second valve group is used for the outflow of waste liquid or product distillate.

6. The dual-column circulating chromatography system according to claim 5, characterized in that: The first, third, fifth, and seventh control valves are opened, while the second, fourth, sixth, and eighth control valves are closed, and the fluid in the system flows sequentially through the first and second chromatographic columns. or The first, third, fifth, and seventh control valves are closed, while the second, fourth, sixth, and eighth control valves are opened, and the fluid in the system flows sequentially through the second chromatographic column and the first chromatographic column.

7. The dual-column circulating chromatography system according to claim 5, characterized in that: When the first and sixth control valves are opened, the second, third, fourth, fifth, seventh, and eighth control valves are closed, and the fluid in the system flows only through the first chromatographic column. or When the fourth and seventh control valves are opened, the first, second, third, fifth, sixth, and eighth control valves are closed, and the fluid in the system flows only through the second chromatographic column.

8. The dual-column circulating chromatography system according to claim 5, characterized in that: The third, fourth, fifth, and sixth control valves are closed, while the first, second, seventh, and eighth control valves are opened, and the fluid in the system flows through the connecting pipeline between the first and second chromatographic columns. or The third, fourth, fifth, and sixth control valves are opened, while the first, second, seventh, and eighth control valves are closed, and the fluid in the system flows through the connecting pipeline between the first and second chromatographic columns.

9. The dual-column circulating chromatography system according to claim 5, characterized in that: The first, fourth, sixth, and seventh control valves are opened, while the second, third, fifth, and eighth control valves are closed, and the fluid in the system flows through the first and second chromatographic columns respectively.

10. The dual-column circulating chromatography system according to claim 5, characterized in that: The multi-channel connector is at least a three-channel connector; detectors are installed on the outlet lines of the first and second chromatographic columns.

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