Fecal coliform group detection device based on fluorescence induced spectrum analysis technology

Abstract

The utility model relates to the technical field of detection equipment, and discloses a fecal coliform group detection device based on a fluorescence induced spectrum analysis technology, which comprises a machine body, a sliding table is arranged on the upper surface of the machine body in a sliding manner, a rotating column is fixedly arranged on the upper surface of the sliding table, and a rotating table is arranged on the outer wall of the top end of the rotating column in a penetrating and rotating manner; and a conversion assembly is arranged in the machine body. Through arrangement of the conversion assembly and cooperation with rotation of the rotary table, the mounting cylinder can rotate on the rotary table under the action of the half gear ring, when the half gear ring is disengaged from contact with the gear ring, due to the elastic force of the coil spring, the mounting cylinder can rotate reversely, and the mounting cylinder can move in the arc-shaped groove through the arc-shaped sliding block; the half gear rings rotating in the reverse direction do not generate large impact force on the gear ring, so that a culture medium in the mounting cylinder can repeatedly shake, flora in a water sample is promoted to be dyed, and the inspection efficiency of the equipment is improved.

Description

Technical Field

[0001] This utility model relates to the field of detection equipment technology, specifically a fecal coliform detection device based on fluorescence-induced spectral analysis technology. Background Technology

[0002] Fecal coliforms belong to the thermostable coliform group and can ferment lactose to produce acid and gas at 44.5℃. They mainly include Escherichia coli (such as Escherichia coli) and Klebsiella spp.

[0003] According to a public notice (No. CN219991603U) of a fecal coliform detection device that is easy to extract, the above application uses test strips to detect fecal coliforms in water samples and finally obtains the results of the water sample's contamination status.

[0004] However, in actual use, the test strips of the aforementioned devices have low accuracy and limited shelf life, making it difficult to obtain accurate and efficient data when testing water samples. Therefore, existing technologies use fluorescence-induced spectroscopy for detection. However, during the testing process, samples need to be pretreated. When pretreating multiple samples, the mixing efficiency between the fluorescent substrate and fecal coliforms in the water sample is limited, which can easily affect the fecal coliform detection data. In view of this, we propose a fecal coliform detection device based on fluorescence-induced spectroscopy. Utility Model Content

[0005] The purpose of this invention is to provide a fecal coliform detection device based on fluorescence-induced spectral analysis technology to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a fecal coliform detection device based on fluorescence-induced spectral analysis technology, comprising a body, a slide table slidably mounted on the upper surface of the body, a rotating column fixedly mounted on the upper surface of the slide table, a turntable rotatably mounted through the outer wall of the top of the rotating column, and a conversion component disposed inside the body;

[0007] The conversion component includes a stepper motor, which is fixedly mounted on the inner wall of the machine body. A threaded rod is fixedly mounted on the output end of the stepper motor, and the outer wall of the threaded rod is threadedly connected to the slide table. A drive motor is fixedly mounted on the inner wall of the slide table, and the output end of the drive motor passes through a rotating column and is fixedly connected to the turntable. A gear ring is fixedly mounted through and on the rotating column. An installation cylinder is rotatably mounted on the inner wall of the turntable. A coil spring is sleeved on the arc-shaped outer wall of the installation cylinder. An arc-shaped groove is formed on the bottom end face of the installation cylinder. An arc-shaped slider is slidably mounted on the inner surface of the arc-shaped groove. An arc-shaped spring is fixedly connected to the side of the arc-shaped slider. A half-tooth ring is fixedly mounted on the lower surface of the arc-shaped slider.

[0008] Preferably, the rotating column has a circular hole inside that matches the outer diameter of the output shaft of the drive motor, and the output end of the drive motor is located in the circular hole. At the same time, the top surface of the output end of the drive motor is fixedly connected to a cylindrical block rotatably mounted on the top of the rotating column, and the outer surface of the cylindrical block penetrates and is fixedly connected to the turntable, so that when the drive motor starts, it can drive the turntable to rotate on the outside of the top of the rotating column.

[0009] Preferably, the turntable has an annular groove inside, and the arc-shaped outer wall of the mounting cylinder is provided with a positioning ring whose thickness matches the annular groove, thereby ensuring the relative stability of the mounting cylinder installed inside the turntable in the vertical direction.

[0010] Preferably, an arc-shaped rod with the same curvature as the arc-shaped groove is fixedly installed on the inner surfaces of both ends of the arc-shaped groove, and the arc-shaped slider passes through the arc-shaped rod and is slidably connected to the arc-shaped rod, and the arc-shaped spring is sleeved on the outside of the arc-shaped rod.

[0011] Preferably, a positioning component is provided on the arc-shaped inner wall of the mounting cylinder. The positioning component includes a spiral band, which is fixedly installed on the arc-shaped inner wall of the mounting cylinder. A notch is formed on the arc-shaped surface of the spiral band.

[0012] Preferably, the spiral strip is made of a spiral silicone strip, and the number of notches is set to a plurality of notches, which are arranged in a spiral linear array on the outer surface of the spiral strip near the center of the mounting cylinder.

[0013] Compared with the prior art, this utility model provides a fecal coliform detection device based on fluorescence-induced spectral analysis technology, which has the following beneficial effects:

[0014] 1. This fecal coliform detection device based on fluorescence-induced spectral analysis technology, through the setting of a conversion component, works in conjunction with the rotation of the turntable. Under the action of the semi-toothed ring, the mounting cylinder will rotate on the turntable. When the semi-toothed ring disengages from the gear ring, the elastic force of the coil spring causes the mounting cylinder to rotate in the opposite direction. Through the movement of the arc-shaped slider in the arc-shaped groove, the reverse-rotating semi-toothed ring will not exert a large impact force on the gear ring, thereby allowing the culture medium in the mounting cylinder to be repeatedly shaken, promoting the staining of bacteria in the water sample and improving the testing efficiency of the equipment.

[0015] 2. This fecal coliform detection device based on fluorescence-induced spectral analysis technology is equipped with a positioning component. The spiral ribbon itself has a certain deformation capacity, and several notched grooves are incorporated to increase the relative stability of the culture dish when placed inside the mounting cylinder. This prevents the dish from tipping over or detaching during rotation and repeated shaking of the mounting cylinder to facilitate staining of fecal coliforms. This reduces manual adjustment and intervention, and avoids external interference with the water sample, thus ensuring the accuracy of the fecal coliform detection data. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main structure of the present utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the present invention without the body;

[0018] Figure 3 This is a schematic diagram of the bottom structure of the turntable of this utility model;

[0019] Figure 4 This is a partial three-dimensional schematic diagram of the conversion component of this utility model;

[0020] Figure 5 This is a partial explosion diagram of the bottom of the mounting cylinder of this utility model.

[0021] In the diagram: 1. Body; 2. Slide table; 3. Rotating column; 4. Turntable; 5. Conversion assembly; 51. Stepper motor; 52. Threaded rod; 53. Drive motor; 55. Gear ring; 56. Mounting cylinder; 57. Coil spring; 58. Arc groove; 59. Arc slider; 510. Arc spring; 511. Half-tooth ring; 6. Positioning assembly; 61. Spiral belt; 62. Notch groove. Detailed Implementation

[0022] like Figures 1-5As shown, this utility model provides a technical solution: a fecal coliform detection device based on fluorescence-induced spectral analysis technology, including a body 1, a slide table 2 slidably mounted on the upper surface of the body 1, a rotating column 3 fixedly mounted on the upper surface of the slide table 2, a turntable 4 rotatably mounted through the outer wall of the top of the rotating column 3, and a conversion component 5 provided inside the body 1. The conversion component 5 includes a stepper motor 51, a threaded rod 52, a drive motor 53, a gear ring 55, a mounting cylinder 56, a coil spring 57, an arc groove 58, an arc slider 59, an arc spring 510, and a semi-tooth ring 511.

[0023] In one embodiment of this utility model, a stepper motor 51 is fixedly installed on the inner wall of the machine body 1. A threaded rod 52 is fixedly installed at the output end of the stepper motor 51. The outer wall of the threaded rod 52 is threadedly connected to the slide table 2. A drive motor 53 is fixedly installed on the inner wall of the slide table 2. The output end of the drive motor 53 passes through the rotating column 3 and is fixedly connected to the turntable 4. A gear ring 55 is fixedly installed through the rotating column 3. An installation cylinder 56 is rotatably installed on the inner wall of the turntable 4. A coil spring 57 is sleeved on the arc-shaped outer wall of the installation cylinder 56. An arc-shaped groove 58 is opened on the bottom end face of the installation cylinder 56. An arc-shaped slider 59 is slidably installed on the inner surface of the arc-shaped groove 58. An arc-shaped spring 510 is fixedly connected to the side of the arc-shaped slider 59. A half-tooth ring 511 is fixedly installed on the lower surface of the arc-shaped slider 59.

[0024] Furthermore, the internal structure of the machine 1 is equipped with a fluorescent dye dispensing device and a high-throughput spectrometer. The fluorescent dye dispensing device is located above the turntable 4. When the turntable 4 rotates the mounting cylinder 56 to a position below the fluorescent dye dispensing device, the fluorescent dye dispensing device is activated to inject the fluorescent dye into the culture medium placed in the mounting cylinder 56, so as to promote the mixing of fecal coliforms with the fluorescent dye. The membrane permeability of live cells and dead cells is different, resulting in different dye binding. Different fluorescence signals (such as green or red) are presented under different excitation wavelengths. The high-throughput spectrometer distinguishes between live and dead bacteria. After the staining pretreatment is completed, the stepper motor 51 is activated, which, together with the rotation of the threaded rod 52, transports the slide 2 and the turntable 4 to the detection area of ​​the high-throughput spectrometer to detect the fecal coliforms in the culture dish placed in the mounting cylinder 56.

[0025] Meanwhile, the slide 2 has an internal mounting cavity to facilitate the fixed installation of the drive motor 53. Additionally, the rotating column 3 has an internal circular hole that matches the outer diameter of the output shaft of the drive motor 53, and the output end of the drive motor 53 is positioned within this circular hole. The top surface of the output end of the drive motor 53 is fixedly connected to a cylindrical block rotatably mounted on the top of the rotating column 3, while the outer surface of the cylindrical block penetrates and is fixedly connected to the turntable 4. This allows the drive motor 53 to rotate the turntable 4 on the outer side of the top of the rotating column 3 when started, thus enabling the mounting of the drive motor 53 within the turntable 4 to proceed smoothly. The mounting cylinder 56 and the gear ring 55 fixedly installed on the outside of the rotating column 3 can rotate relative to each other. Furthermore, the turntable 4 has an annular groove inside, and the arc-shaped outer wall of the mounting cylinder 56 is provided with a positioning ring whose thickness matches the annular groove, thereby ensuring the relative stability of the mounting cylinder 56 installed inside the turntable 4 in the vertical direction. Specifically, the two ends of the coil spring 57 are fixedly connected to the bottom inner wall of the annular groove and the arc-shaped outer surface of the positioning ring, respectively, so that the mounting cylinder 56 always has a tendency to rotate in the opposite direction to achieve reset when it rotates inside the turntable 4.

[0026] In addition, an arc-shaped rod with the same curvature as the arc-shaped groove 58 is fixedly installed on the inner surface of both ends of the arc-shaped groove 58, and an arc-shaped slider 59 passes through the arc-shaped rod and is slidably connected to the arc-shaped rod. An arc-shaped spring 510 is sleeved on the outer side of the arc-shaped rod. At the same time, the inner surface of the semi-tooth ring 511 is in contact with the bottom end face of the mounting cylinder 56, and the teeth of the semi-tooth ring 511 mesh with the gear ring 55 to realize transmission. Fluorescent dye is added to the culture medium installed in the mounting cylinder 56, and the fecal coliforms in the test sample water are mixed with the fluorescent dye to stain the bacteria.

[0027] Specifically, a positioning component 6 is provided on the arc-shaped inner wall of the mounting cylinder 56. The positioning component 6 includes a spiral band 61, which is fixedly installed on the arc-shaped inner wall of the mounting cylinder 56. A notch 62 is provided on the arc-shaped surface of the spiral band 61.

[0028] In this embodiment of the invention, the spiral strip 61 is made of a spiral silicone strip, and a plurality of notched grooves 62 are provided. The plurality of notched grooves 62 are arranged in a spiral linear array on the outer surface of the spiral strip 61 near the center of the mounting cylinder 56. Thus, by utilizing the deformation capability of the spiral strip 61 material itself, combined with the arrangement of the plurality of notched grooves 62, the relative stability of the culture dish when placed in the mounting cylinder 56 is increased. This prevents the dish from tipping over or falling off when the mounting cylinder 56 rotates and shakes repeatedly to facilitate the staining of fecal coliforms, reducing manual adjustment and intervention, and avoiding interference from external factors that could affect the accuracy of the fecal coliform detection data.

[0029] In this invention, during use, equal amounts of water samples are placed in petri dishes, which are then placed inside mounting cylinders 56 and secured using spiral bands 61. The stepper motor 51 drives the rotation of the threaded rod 52, pushing the turntable 4 below the fluorescent dye dispensing device. The fluorescent dye dispensing device is then activated, dispensing the fluorescent dye into the culture medium placed in the mounting cylinders 56. The drive motor 53 then drives the turntable 4 to rotate, sequentially dispensing the fluorescent dye into the multiple mounting cylinders 56 on the turntable 4. The drive motor 53 continues to rotate, and due to the relative rotation of the mounting cylinders 56 and the gear ring 55, the semi-gear ring 5... Under the action of 11, the mounting cylinder 56 will rotate on the turntable 4. When the semi-tooth ring 511 disengages from the gear ring 55, the mounting cylinder 56 will rotate in the opposite direction due to the elastic force of the coil spring 57. The movement of the arc-shaped slider 59 in the arc-shaped groove 58 prevents the semi-tooth ring 511 from exerting a large impact force on the gear ring 55, thereby allowing the culture medium in the mounting cylinder 56 to shake repeatedly, promoting the staining of bacteria in the water sample and improving the testing efficiency of the equipment. After mixing, the stepper motor 51 is started to drive the slide table 2 to the detection area inside the machine body 1. The fecal coliforms in the water sample are detected by the high-throughput spectrometer built into the machine body 1.

[0030] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the protection scope of the present invention.

Claims

1. A fecal coliform detection device based on fluorescence-induced spectral analysis technology, comprising a body (1), wherein a slide (2) is slidably mounted on the upper surface of the body (1), a rotating column (3) is fixedly mounted on the upper surface of the slide (2), and a turntable (4) is rotatably mounted through the top outer wall of the rotating column (3), characterized in that: The internal structure (1) is equipped with a conversion component (5); The conversion component (5) includes a stepper motor (51), which is fixedly mounted on the inner wall of the machine body (1). A threaded rod (52) is fixedly mounted on the output end of the stepper motor (51). The outer wall of the threaded rod (52) is threadedly connected to the slide (2). A drive motor (53) is fixedly mounted on the inner wall of the slide (2). The output end of the drive motor (53) passes through the rotating column (3) and is fixedly connected to the turntable (4). A gear ring (55) is fixedly installed. An installation cylinder (56) is rotatably installed on the inner wall of the turntable (4). A coil spring (57) is sleeved on the arc-shaped outer wall of the installation cylinder (56). An arc-shaped groove (58) is opened on the bottom end face of the installation cylinder (56). An arc-shaped slider (59) is slidably installed on the inner surface of the arc-shaped groove (58). An arc-shaped spring (510) is fixedly connected to the side of the arc-shaped slider (59). A half-tooth ring (511) is fixedly installed on the lower surface of the arc-shaped slider (59).

2. The device for detecting fecal coliforms based on fluorescence induction spectroscopy according to claim 1, characterized in that: The rotating column (3) has a circular hole inside that matches the outer diameter of the output shaft of the drive motor (53), and the output end of the drive motor (53) is located in the circular hole. At the same time, the top surface of the output end of the drive motor (53) is fixedly connected to the cylindrical block rotatably mounted on the top of the rotating column (3), and the outer surface of the cylindrical block is connected to the turntable (4) through and fixedly connected. 3.The device for detecting fecal coliforms based on fluorescence induction spectroscopy according to claim 1, characterized in that: The turntable (4) has an annular groove inside, and the mounting cylinder (56) has a positioning ring with a thickness that matches the annular groove on its arc-shaped outer wall.

4. The fecal coliform detection device based on fluorescence-induced spectral analysis technology according to claim 1, characterized in that: An arc-shaped rod with the same curvature as the arc-shaped groove (58) is fixedly installed on the inner surface of both ends of the arc-shaped groove (58), and the arc-shaped slider (59) passes through the arc-shaped rod and is slidably connected to the arc-shaped rod. The arc-shaped spring (510) is sleeved on the outside of the arc-shaped rod.

5. The fecal coliform detection device based on fluorescence-induced spectral analysis technology according to claim 1, characterized in that: A positioning component (6) is provided on the arc-shaped inner wall of the mounting cylinder (56). The positioning component (6) includes a spiral band (61). The spiral band (61) is fixedly installed on the arc-shaped inner wall of the mounting cylinder (56). A notch (62) is provided on the arc-shaped surface of the spiral band (61).

6. The device for detecting fecal coliforms based on fluorescence induction spectroscopy according to claim 5, characterized in that: The spiral strip (61) is made of spiral silicone strip, and the number of notches (62) is set to a certain number. The notches (62) are arranged in a spiral linear array on the outer surface of the spiral strip (61) near the center of the mounting cylinder (56).

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