Multi-modal microscopic image acquisition device

Abstract

The utility model discloses a multi-mode microscopic image acquisition device which comprises a mounting platform, a translation driving mechanism mounted on the mounting platform, and a sample cabin assembly connected with the translation driving mechanism, a first through hole is formed in the top of the mounting platform, and a light source bright field assembly is further arranged at the position, above the first through hole, of the top of the mounting platform; a Z-axis lifting mechanism is further mounted at the bottom of the mounting platform, and the Z-axis lifting mechanism is further in driving connection with a fluorescence imaging assembly; the fluorescence imaging assembly is arranged below the first through hole; a sample uniform mixing assembly is also mounted on the mounting platform. According to the utility model, sample processing assemblies for uniform mixing, temperature regulation and the like are integrated, bright field signals, fluorescence signals and absorbance of various spectrums can be simultaneously acquired, and a basic technical tool is provided for multi-parameter characterization of complex biological samples.

Description

Technical Field

[0001] This utility model relates to the field of microscopic imaging technology, and in particular to a multimodal microscopic image acquisition device. Background Technology

[0002] With the rapid development of life sciences and clinical medicine, traditional single-modal microscopy imaging techniques are no longer sufficient to meet the needs of multi-dimensional characterization of complex samples. Multi-method research requires the simultaneous acquisition of multiple physical signals (such as fluorescence, multispectral data, and optical density) while performing imaging.

[0003] To balance versatility and cost-effectiveness, existing commercial multispectral microscopy systems mostly employ filter wheels for spectral dispersion. However, this approach has significant drawbacks: mechanical filter switching limits temporal resolution (typical switching time > 50 ms), making it unable to capture fast dynamic processes. Utility Model Content

[0004] The purpose of this invention is to provide a multimodal microscopic image acquisition device. This device integrates sample processing components such as mixing and temperature control, and can simultaneously acquire bright field signals, fluorescence signals and absorbance of multiple spectra, providing a basic technical tool for multi-parameter characterization of complex biological samples.

[0005] To achieve the above objectives, the following technical solution is adopted:

[0006] A multimodal microscopic image acquisition device includes a mounting platform, a translation drive mechanism mounted on the mounting platform, and a sample chamber assembly connected to the translation drive mechanism. The mounting platform has a first through-hole at its top, and a bright-field light source assembly is arranged above the first through-hole at the top of the mounting platform. A Z-axis lifting mechanism is also mounted at the bottom of the mounting platform, and the Z-axis lifting mechanism is also driven and connected to a fluorescence imaging assembly. The fluorescence imaging assembly is arranged below the first through-hole. A sample mixing assembly is also mounted on the mounting platform.

[0007] Furthermore, the sample chamber assembly includes a sample stage and a heating plate; the sample stage includes a first connecting seat connected to a translation drive mechanism, and one end of each side of the first connecting seat is integrally connected to a support seat; the two support seats are arranged parallel to each other, and one end of each support seat extends outward away from the first connecting seat; the first through hole is located between the two support seats; a clamping assembly is also installed on each support seat; the heating plate is installed at the bottom of the first connecting seat, and one end of the heating plate is forked into two heating parts, which extend to the bottom of a support seat respectively.

[0008] Furthermore, each of the two bearing seats extends outward from the lower part on the opposite side of the bearing seat; the clamping assembly includes an elastic clamp; the elastic clamp is arranged along the length direction of the bearing platform, and one end of the elastic clamp is installed on the top of the bearing seat, and the other end of the elastic clamp is bent downward and extended to the bearing platform; the end of the elastic clamp extending to the bearing platform is also bent upward.

[0009] Furthermore, the sample chamber assembly also includes a temperature-controlled cover mounted on two support seats, and the temperature-controlled cover is also equipped with light-transmitting glass.

[0010] Furthermore, the translation drive mechanism includes a Y-axis translation mechanism installed at the bottom of the installation platform, a Y-axis slide rail arranged at the top of the installation platform, a Y-axis slide table slidably arranged on the Y-axis slide rail and connected to the Y-axis translation mechanism, and an X-axis translation mechanism installed at the top of the Y-axis slide table; the top of the Y-axis slide table is also provided with an X-axis slide rail, and a first connecting seat is slidably arranged on the X-axis slide rail and connected to the X-axis translation mechanism.

[0011] Furthermore, a first bracket is installed on the top of the mounting platform, and a mounting plate is connected to the first bracket; the light source bright field component includes a white light source component installed on the mounting plate, and a red and green light source component installed on the white light source component; a barcode scanner is also installed on one side of the mounting plate.

[0012] Furthermore, a fixed base is connected to the bottom of the mounting platform, and the Z-axis lifting mechanism is mounted on the fixed base; the fluorescence imaging component includes a Z-axis slide stage, a fluorescence light source component, and an imaging component; a Z-axis slide rail is also arranged vertically on one side of the fixed base, and the Z-axis slide stage is slidably arranged on the Z-axis slide rail and connected to the Z-axis lifting mechanism; the imaging component is arranged vertically on one side of the Z-axis slide stage, and the fluorescence light source component is mounted on the imaging component.

[0013] Furthermore, the sample mixing assembly includes a mixing motor, a connecting cylinder, and a mixing magnet; a second through hole is also provided at the top of the mounting platform near the first through hole, the mixing motor is installed at the bottom of the mounting platform, and the output shaft of the mixing motor is inserted into the second through hole; the connecting cylinder is installed on the output shaft of the mixing motor, and an installation cylinder is also connected to the outer wall of the connecting cylinder; the upper part of the installation cylinder extends upward from the second through hole, and the mixing magnet is installed inside the installation cylinder.

[0014] Furthermore, it also includes a magnetization assembly; the magnetization assembly includes a fixing bracket and a fixing magnet; the fixing bracket is detachably mounted on the fluorescence imaging assembly, and the top of the fixing bracket is also provided with an installation groove; the fixing magnet is installed in the installation groove.

[0015] Furthermore, a support frame is connected to the bottom of the fixed base; the main control board assembly and a drive board assembly connected to the main control board assembly are also installed on the support frame.

[0016] By adopting the above solution, the beneficial effects of this utility model are:

[0017] This invention integrates sample processing components such as mixing and temperature control, and can simultaneously acquire bright-field signals, fluorescence signals and absorbance of multiple spectra, providing a basic technical tool for multi-parameter characterization of complex biological samples. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of this utility model;

[0019] Figure 2 for Figure 1 A structural diagram omitting some of the organizational structures;

[0020] Figure 3 This is a schematic diagram of the Z-axis lifting mechanism and fluorescence imaging component of this utility model;

[0021] Figure 4 This is an exploded view of the fluorescence imaging component of this utility model;

[0022] Figure 5 This is a cross-sectional view of the light source bright field component of this utility model;

[0023] Figure 6 This is an exploded view of the sample chamber assembly of this utility model;

[0024] Figure 7 An exploded view of the sample mixing component of this utility model;

[0025] Figure 8 This is an exploded view of the magneto-solid component of this utility model.

[0026] The following are explanations of the labels in the attached diagram:

[0027] 1. Mounting platform; 2. Translation drive mechanism; 3. Sample chamber assembly; 4. Bright field light source assembly; 5. Z-axis lifting mechanism; 6. Fluorescence imaging assembly; 7. Sample mixing assembly; 8. Magnetization assembly; 11. First through hole; 12. First bracket; 13. Mounting cross plate; 14. Fixing seat; 21. Y-axis translation mechanism; 22. Y-axis slide rail; 23. Y-axis slide stage; 24. X-axis translation mechanism; 25. X-axis slide rail; 31. Heating plate; 32. First connecting seat; 33. Bearing seat; 34. Elastic clamp; 35. Bearing stage; 36. Constant temperature cover; 37. Transparent glass; 41. White light source assembly; 42. Red and green light source assembly; 43. Barcode scanner; 61. Z-axis slide stage; 62. Fluorescence light source assembly; 63. Imaging. Components; 71. Mixing motor; 72. Connecting cylinder; 73. Mixing magnet; 74. Mounting cylinder; 81. Fixing bracket; 82. Fixing magnet; 141. Support frame; 142. Main control board assembly; 143. Drive board assembly; 311. Heating unit; 411. White light lens barrel; 412. First beam splitter; 413. First LED light board; 421. First light source holder; 422. Second beam splitter; 423. Red filter; 424. Green filter; 425. Second LED light board; 426. Third LED light board; 621. Fluorescent lens barrel; 622. Fourth LED light board; 631. Objective lens; 632. Optical tee; 633. Beam splitter assembly; 634. Imaging lens barrel; 635. CMOS board. Detailed Implementation

[0028] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] Reference Figures 1 to 8 As shown, this utility model provides a multimodal microscopic image acquisition device. In one embodiment, it includes a mounting platform 1, a translation drive mechanism 2 mounted on the mounting platform 1, and a sample chamber assembly 3 connected to the translation drive mechanism 2. The top of the mounting platform 1 has a first through hole 11, and a light source bright field assembly 4 is arranged above the first through hole 11 on the top of the mounting platform 1. A Z-axis lifting mechanism 5 is also installed at the bottom of the mounting platform 1, and the Z-axis lifting mechanism 5 is also driven and connected to a fluorescence imaging assembly 636. The fluorescence imaging assembly 636 is arranged below the first through hole 11. A sample mixing assembly 7 is also installed on the mounting platform 1, and a magnetizing assembly 8 is also installed on the fluorescence imaging assembly 636.

[0030] In this embodiment, the sample chamber assembly 3 is used to load the microfluidic chip to be tested, the bright field light source assembly 4 is used to provide a bright field light source, the fluorescence imaging assembly 636 is used to provide a fluorescence light source and imaging, the sample mixing assembly 7 is used to drive the stir bar pre-placed in the microfluidic chip reaction chamber (in one embodiment, it is an optical density cavity opened in the microfluidic chip) to move, so that the sample in the reaction chamber is mixed with the pre-loaded dry reagent, and the magnetization assembly is used to adsorb and fix the stir bar in the reaction chamber when the fluorescence imaging assembly performs imaging, so as to avoid affecting the imaging quality; the translation drive mechanism 2 can drive the sample chamber assembly 3 to move the microfluidic chip along the X-axis and Y-axis to realize the acquisition of images at different positions of the microfluidic chip. At the same time, the Z-axis lifting mechanism 5 can drive the fluorescence imaging assembly 636 to move up and down to adjust the focal length and ensure the clarity and quality of the imaging.

[0031] In one embodiment, the sample chamber assembly 3 includes a sample stage and a heating plate 31; the sample stage includes a first connecting seat 32 connected to the translation drive mechanism 2, and one end of each side of the first connecting seat 32 is integrally connected to a support seat 33; the two support seats 33 are arranged parallel to each other, and one end of the two support seats 33 extends outward in a direction away from the first connecting seat 32; the first through hole 11 is located between the two support seats 33; a clamping assembly is also installed on each support seat 33; the heating plate 31 is installed at the bottom of the first connecting seat 32.

[0032] In this embodiment, the sample stage is a machined aluminum alloy part with a hard anodized surface to improve wear resistance. Additionally, each of the two support seats 33 extends outward from its lower side by a support platform 35. The clamping assembly includes an elastic clamping piece 34. The elastic clamping piece 34 is arranged along the length of the support platform 35, with one end of the elastic clamping piece 34 mounted on the top of the support seat 33 and the other end bent downward and extending onto the support platform 35. The end of the elastic clamping piece 34 extending onto the support platform 35 is also bent upward.

[0033] The elastic clip 34 is made of a flexible material, such as... Figure 2 , 6As shown, one end of the elastic clip 34 is fixed to the top of the support 33, and the other end bends downward and extends to the support platform 35. Simultaneously, the end of the elastic clip 34 extending towards the support platform 35 is also bent upward, creating a downwardly protruding clamping part at the bottom of the elastic clip 34. When loading the microfluidic chip, the upwardly bent end of the elastic clip 34 can be lifted first to separate it from the support platform 35. Then, both ends of the microfluidic chip are placed on the support platform 35. Subsequently, the elastic clip 34 is released, returning to its original position. The clamping part then presses and limits the microfluidic chip, ensuring its stable installation. In addition, a heating plate 31 is installed at the bottom of the sample stage to heat the microfluidic chip placed on the support stage 35 to meet the temperature requirements for sample detection. At the same time, to improve the heating effect, one end of the heating plate 31 is also branched into two heating parts 311, which extend to the bottom of a support seat 33 respectively. Furthermore, the sample chamber assembly 3 also includes a constant temperature cover 36 installed on the two support seats 33, and a light-transmitting glass 37 is installed on the constant temperature cover 36. The constant temperature cover 36 plays a role in heat preservation to ensure that the temperature of the sample remains stable during the detection process.

[0034] In one embodiment, the translation drive mechanism 2 includes a Y-axis translation mechanism 21 mounted on the bottom of the mounting platform 1, a Y-axis slide rail 22 arranged on the top of the mounting platform 1, a Y-axis slide table 23 slidably arranged on the Y-axis slide rail 22 and connected to the Y-axis translation mechanism 21, and an X-axis translation mechanism 24 mounted on the top of the Y-axis slide table 23; the top of the Y-axis slide table 23 is also provided with an X-axis slide rail 25, and a first connecting seat 32 is slidably arranged on the X-axis slide rail 25 and connected to the X-axis translation mechanism 24.

[0035] Continue to refer to Figure 2 As shown, in this embodiment, the Y-axis translation mechanism 21 and the X-axis translation mechanism 24 can adopt a motor lead screw transmission method, or other mechanisms that can drive the sample stage to perform translational movements. This utility model does not limit this. In a preferred embodiment, two sets of Y-axis slide rails 22 are provided, respectively arranged at one end of the top of the mounting platform 1. The bottom ends of the Y-axis slide table 23 are respectively connected to a Y-axis slide rail 22 via sliders. By setting two sets of Y-axis slide rails 22, the stability of the Y-axis slide table 23's sliding can be ensured. Simultaneously, the mounting platform... The top of the mounting platform 1 is also provided with a first sliding hole along the Y-axis direction. The ball nut on the lead screw of the Y-axis translation mechanism 21 is also equipped with a connecting block. The upper part of the connecting block passes through the first sliding hole and connects to the bottom of the Y-axis slide 23. This connection method can improve the compactness of the structure. In order to improve the accuracy of the movement of the Y-axis slide 23 and limit its movement stroke, a sensing plate is also installed at one end of the Y-axis slide 23. A U-shaped photoelectric sensor that works with the sensing plate is set at each end of the mounting platform 1 near the sensing plate.

[0036] The first connecting seat 32 of the sample stage is slidably arranged on the X-axis slide rail 25. The X-axis translation mechanism 24 is connected to the top of the first connecting seat 32 via a ball nut mounted on the lead screw. At the same time, in order to improve the accuracy of the sample stage movement and limit its movement stroke, a sensing plate is also installed at one end of the first connecting seat 32. A U-shaped photoelectric sensor that works with the sensing plate is set at each end of the Y-axis slide 23 near the sensing plate.

[0037] In one embodiment, a first bracket 12 is also installed on the top of the mounting platform 1, and a mounting horizontal plate 13 is also connected to the first bracket 12; the light source bright field component 4 includes a white light source component 41 installed on the mounting horizontal plate 13, and a red and green light source component 42 installed on the white light source component 41; a barcode scanner 43 (used to scan and identify the sample information to be detected) is also installed on one side of the mounting horizontal plate 13.

[0038] In this embodiment, the white light source assembly 41 and the red-green light source assembly 42 can be directly adopted from existing products, and this utility model does not limit them. In a feasible embodiment, the white light source assembly 41 includes a white light tube 411 mounted on the mounting plate 13, a first beam splitter mount mounted inside the white light tube 411, a first beam splitter 412 mounted at an angle of 45° inside the first beam splitter mount, and a first LED light panel 413 mounted on the top of the white light tube 411. The white light emitted by the first LED light panel 413 passes through the first beam splitter 412 and then exits from the white light tube 411. The bottom of the tube 411 emits light towards the microfluidic chip; the red and green light source assembly 42 includes a first light source base 421, and one end of the first light source base 421 has a first light exit hole; the outer wall of the white light tube 411 has a first light entrance hole corresponding to the reflective surface of the first beam splitter 412, the first light source base 421 is installed on the outer wall of the white light tube 411, and the first light exit hole and the first light entrance hole are aligned; a second beam splitter base is provided inside the first light source base 421, and a second beam splitter 422 is installed at an angle of 45° inside the second beam splitter base; the top and one end of the second beam splitter base are... A red filter 423 and a green filter 424 are installed. A second LED light panel 425 is installed on the top of the first light source base 421, corresponding to the red filter 423. A third LED light panel 426 is installed on the other end of the first light source base 421, corresponding to the green filter 424. The white light emitted by the second LED light panel 425 passes through the red filter 423 and becomes red light. The red light is then reflected by the second beam splitter 422 and enters the first light-emitting aperture and the first light-receiving aperture, then enters the reflecting surface of the first beam splitter 412. After being reflected by the first beam splitter 412, the red light is separated from the white light. The bottom of the lens tube 411 emits light towards the microfluidic chip; the white light emitted by the third LED light panel 426 is filtered through the green filter 424 and becomes green light. The green light passes directly through the second beam splitter 422, and then enters the reflective surface of the first beam splitter 412 through the first light outlet and the first light inlet. After being reflected by the first beam splitter 412, the green light is emitted from the bottom of the white light lens tube 411 towards the microfluidic chip. In addition, one end of the mounting plate 13 is connected to the first bracket 12 by an adjusting screw. The position of the mounting plate 13 can be adjusted by adjusting the screw, thereby adjusting the coaxiality of the optical axis.

[0039] In one embodiment, the bottom of the mounting platform 1 is also connected to a fixed base 14, and the Z-axis lifting mechanism 5 is mounted on the fixed base 14; the fluorescence imaging component 636 includes a Z-axis slide 61, a fluorescence light source component 62, and an imaging component 63; a Z-axis slide rail is also arranged vertically on one side of the fixed base 14, and the Z-axis slide 61 is slidably arranged on the Z-axis slide rail and connected to the Z-axis lifting mechanism 5; the imaging component 63 is arranged vertically on one side of the Z-axis slide 61, and the fluorescence light source component 62 is mounted on the imaging component 63.

[0040] In this embodiment, the Z-axis lifting mechanism 5 adopts a motor lead screw transmission method, which is not limited in this invention; the fluorescent light source assembly 62 and the imaging assembly 63 can directly use existing products. In a feasible embodiment, the fluorescent light source assembly 62 includes a fluorescent lens barrel 621, a fourth LED light board 622, a light board bracket, an optical lens, and an aperture; the imaging assembly 63 includes an objective lens 631, an optical tee 632, a beam splitter assembly 633, an imaging lens barrel 634, an optical lens, and a CMOS board 635; when installing the beam splitter assembly 633 (i.e., the reflector), the beam splitter bracket can be adjusted according to different width dimensions. To prevent mistakes, first insert the beam splitter assembly 633 into the optical tee 632, then fix the imaging tube 634 and CMOS board 635 in sequence with screws, and fix the objective lens 631 to the optical tee 632 with threads; for the fluorescent light source assembly 62, first install the lens and aperture on the fluorescent tube 621, fix the lamp board bracket to the tube with threads, then fix the fourth LED lamp board 622 to the lamp board bracket with screws, and finally connect and fix the assembled fluorescent light source assembly 62 to the optical tee 632 of the imaging assembly 63 with screws, and finally install the assembled product on the Z-axis slide 61 with clamps.

[0041] In one embodiment, the sample mixing assembly 7 includes a mixing motor 71, a connecting cylinder 72, and a mixing magnet 73; a second through hole is also provided at the top of the mounting platform 1 near the first through hole 11, the mixing motor 71 is mounted on the bottom of the mounting platform 1, and the output shaft of the mixing motor 71 is inserted into the second through hole; the connecting cylinder 72 is mounted on the output shaft of the mixing motor 71, and the outer wall of the connecting cylinder 72 is also connected to the mounting cylinder 74; the upper part of the mounting cylinder 74 extends upward from the second through hole, and the mixing magnet 73 is installed in the mounting cylinder 74. Meanwhile, the magnetization assembly 8 includes a fixing bracket 81 and a fixing magnet 82; the fixing bracket 81 is detachably mounted on the fluorescence imaging assembly 636, and the top of the fixing bracket 81 is also provided with a mounting groove; the fixing magnet 82 is installed in the mounting groove.

[0042] In this embodiment, the magnetized assembly 8 is mounted on the objective lens 631 of the imaging assembly 63. Specifically, the fixing bracket 81 of the magnetized assembly 8 can be fixed to the objective lens 631 by tight fitting and inverting. The stir bar is made of magnetic material. When the mixing motor 71 is running, it can drive the mixing magnet 73 to run. The mixing magnet 73 can drive the stir bar to move by magnetic attraction, thereby realizing the stirring function. At the same time, when the fluorescence imaging assembly acquires images, the fixing magnet 82 can attract and fix the stir bar in the reaction chamber to avoid affecting the imaging quality. In addition, in this embodiment, the mixing motor 71 is not directly connected to the fluorescence imaging assembly 636, which can reduce the impact of mixing vibration on the imaging system. At the same time, the magnetized assembly 8 and the sample mixing assembly 7 are independent of each other and have sufficient distance, which can avoid mutual magnetic interference.

[0043] In one embodiment, a support frame 141 is also connected to the bottom of the fixed base 14; a main control board assembly 142 and a drive board assembly 143 connected to the main control board assembly 142 are also installed on the support frame 141. In this embodiment, the main control board assembly 142 includes a PCBA board and a bracket, and the drive board assembly 143 includes a drive board and a bracket. The bracket is made of plastic, which can provide support strength while also acting as an isolation and buffer. The drive board assembly 143 is connected to the aforementioned motion mechanisms (such as the X-axis translation mechanism 24, the Y-axis translation mechanism 21, the Z-axis lifting mechanism 5, etc.). The main control board assembly 142 and the drive board assembly 143 cooperate with each other to control the operation of the entire device and coordinate the work of each component.

[0044] The above are merely preferred embodiments of this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A multimodal microscopic image acquisition device, characterized in that, The device includes an installation platform, a translation drive mechanism mounted on the installation platform, and a sample chamber assembly connected to the translation drive mechanism. The top of the installation platform has a first through-hole, and a bright-field light source assembly is arranged above the first through-hole. A Z-axis lifting mechanism is also installed at the bottom of the installation platform, and the Z-axis lifting mechanism is also connected to a fluorescence imaging assembly. The fluorescence imaging assembly is arranged below the first through-hole. A sample mixing assembly is also installed on the installation platform.

2. The multimodal microscopic image acquisition device according to claim 1, characterized in that, The sample chamber assembly includes a sample stage and a heating plate. The sample stage includes a first connecting seat connected to a translation drive mechanism, and each end of the first connecting seat is integrally connected to a support seat. The two support seats are arranged parallel to each other, and one end of each support seat extends outward away from the first connecting seat. The first through hole is located between the two support seats. Each support seat is also equipped with a clamping assembly. The heating plate is installed at the bottom of the first connecting seat, and one end of the heating plate is forked into two heating parts, which extend to the bottom of a support seat respectively.

3. The multimodal microscopic image acquisition device according to claim 2, characterized in that, Each of the two bearing seats extends outward from the lower part of opposite sides to form a bearing platform; the clamping assembly includes an elastic clamp; the elastic clamp is arranged along the length of the bearing platform, and one end of the elastic clamp is mounted on the top of the bearing seat, while the other end of the elastic clamp is bent downward and extends to the bearing platform; the end of the elastic clamp extending to the bearing platform is also bent upward.

4. The multimodal microscopic image acquisition device according to claim 3, characterized in that, The sample chamber assembly also includes a temperature-controlled cover mounted on two support seats, and the temperature-controlled cover is also equipped with transparent glass.

5. The multimodal microscopic image acquisition device according to claim 2, characterized in that, The translation drive mechanism includes a Y-axis translation mechanism installed at the bottom of the installation platform, a Y-axis slide rail arranged at the top of the installation platform, a Y-axis slide table slidably arranged on the Y-axis slide rail and connected to the Y-axis translation mechanism, and an X-axis translation mechanism installed at the top of the Y-axis slide table; the top of the Y-axis slide table is also provided with an X-axis slide rail, and a first connecting seat is slidably arranged on the X-axis slide rail and connected to the X-axis translation mechanism.

6. The multimodal microscopic image acquisition device according to claim 1, characterized in that, The top of the mounting platform is also equipped with a first bracket, and a mounting plate is connected to the first bracket; the light source bright field component includes a white light source component mounted on the mounting plate, and a red and green light source component mounted on the white light source component; a barcode scanner is also installed on one side of the mounting plate.

7. The multimodal microscopic image acquisition device according to claim 1, characterized in that, The bottom of the mounting platform is also connected to a fixed base, and the Z-axis lifting mechanism is mounted on the fixed base; the fluorescence imaging component includes a Z-axis slide stage, a fluorescence light source component, and an imaging component; a Z-axis slide rail is also arranged vertically on one side of the fixed base, and the Z-axis slide stage is slidably arranged on the Z-axis slide rail and connected to the Z-axis lifting mechanism; the imaging component is arranged vertically on one side of the Z-axis slide stage, and the fluorescence light source component is mounted on the imaging component.

8. The multimodal microscopic image acquisition device according to claim 1, characterized in that, The sample mixing assembly includes a mixing motor, a connecting cylinder, and a mixing magnet. A second through hole is also provided at the top of the mounting platform near the first through hole. The mixing motor is installed at the bottom of the mounting platform, and the output shaft of the mixing motor is inserted into the second through hole. The connecting cylinder is installed on the output shaft of the mixing motor, and an installation cylinder is also connected to the outer wall of the connecting cylinder. The upper part of the installation cylinder extends upward from the second through hole, and the mixing magnet is installed inside the installation cylinder.

9. The multimodal microscopic image acquisition device according to claim 1, characterized in that, It also includes a magnetization assembly; the magnetization assembly includes a fixing bracket and a fixing magnet; the fixing bracket is detachably mounted on the fluorescence imaging assembly, and the top of the fixing bracket is provided with an installation groove; the fixing magnet is installed in the installation groove.

10. The multimodal microscopic image acquisition device according to claim 7, characterized in that, The bottom of the fixed base is also connected to a support frame; the support frame is also equipped with a main control board assembly and a drive board assembly connected to the main control board assembly.

Images