NK cell immunodetection kit with good sealing performance

Abstract

The utility model relates to the technical field of immunodetection, and discloses an NK (Natural Killer) cell immunodetection kit with good sealing performance, which comprises a kit body, a kit cover and a reagent bottle body, and further comprises a positioning component arranged on the surfaces of the kit body and the kit cover, and a cutter bolted on the surface of the kit cover; the round cover, the vertical column and the rotating column are driven to rotate by pressing the pressing plate, the interior of the reagent bottle is opened to facilitate operation, after operation is finished, the pressing plate is loosened by hands, the torsion spring can push the round cover to automatically reset, the situation that workers forget to seal the reagent bottle body is avoided, and the working efficiency is improved. Meanwhile, before the reagent bottle is started, the round cover and the reagent bottle are fixed under the action of the short rod and the transverse plate, the round cover is prevented from being opened under the action of external force, the sealing effect on the reagent bottle in the kit is improved, the short rod can be cut off by combining with the cutter on the kit cover, and the kit is more convenient to use.

Description

Technical Field

[0001] This utility model relates to the field of immunoassay technology, specifically to a well-sealed NK cell immunoassay kit. Background Technology

[0002] NK cell immunoassay kits are primarily used to assess and detect the functional activity of natural killer (NK) cells. They are widely used in immunological research and clinical diagnostics. Their core functions include: ① efficiently isolating NK cells from peripheral blood using specific reagents (such as antibody magnetic beads) to ensure sample purity; ② providing cytokines (such as IL-2 and IL-15) and culture medium to support in vitro expansion and activity maintenance of NK cells; ③ quantitatively detecting the killing capacity of NK cells, for example, by co-culturing target cells (such as the K562 tumor cell line) and measuring LDH release or fluorescent labeling signals to assess their clearance efficiency against abnormal cells.17 Furthermore, the kits typically include calibrators and standardized reagents to reduce experimental errors, making them suitable for tumor immunotherapy, viral infection research, and immune function assessment.

[0003] After the reagent bottle is opened, staff sometimes forget to seal it immediately after performing the procedure. The longer the inside of the reagent bottle is exposed to the outside, the more easily it becomes contaminated, which in turn affects the test results. Utility Model Content

[0004] The purpose of this invention is to provide an NK cell immunoassay kit with good sealing properties to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a well-sealed NK cell immunoassay kit, comprising a box body, a box cap, and a reagent bottle body, and further comprising:

[0006] Positioning components are provided on the surfaces of the box body and the lid. A cutter is also bolted to the surface of the lid. A round cap is provided on the top of the reagent bottle body. A pressure plate is integrally provided on one side of the round cap. A connecting plate is fused to one side of the reagent bottle body. A rotating column is rotatably connected to the surface of the connecting plate. A vertical column is fixed to the surface of the rotating column, and the top of the vertical column is fixed to the bottom of the connecting plate.

[0007] A torsion spring is fixed to one side of the connecting plate, and the other end of the torsion spring is in contact with the vertical column. Horizontal plates are also fixed to both sides of the reagent bottle body. Short rods are fused to both sides of the round cap, and the other ends of the short rods are fused to the horizontal plates.

[0008] Preferably, the positioning component includes a connecting rod, a through hole, and a sliding sleeve. The sliding sleeve is bolted to the box body, and the connecting rod is bolted to the box cover. The through hole is formed on the surface of the connecting rod. A pin is slidably connected to the inner wall of the sliding sleeve. A ring is fixed to the surface of the pin. A compression spring is fixed to one side of the ring, and the other end of the compression spring is fixed to the sliding sleeve.

[0009] Preferably, a ring is also fused to one side of the pin.

[0010] Preferably, the inner diameter of the through hole is the same as the inner diameter of the sliding sleeve.

[0011] Preferably, the short rod is made of plastic.

[0012] Preferably, the top of the pressure plate is provided with several anti-slip ridges.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] This invention uses a pressing plate to rotate the round cap, vertical column, and rotating column, opening the reagent bottle for operation. After operation, releasing the pressing plate allows the torsion spring to automatically reset the round cap, preventing staff from forgetting to seal the reagent bottle and contaminating the internal liquid. Before use, the short rod and horizontal plate secure the round cap to the bottle, preventing it from opening under external force and enhancing the seal. The cutter on the lid allows for easy cutting of the short rod, making it even more convenient to use. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0016] Figure 2 This utility model Figure 1 Enlarged structural diagram at point A;

[0017] Figure 3 This is a schematic diagram of the box lid after it is closed in this utility model;

[0018] Figure 4 This utility model Figure 3 Enlarged structural diagram at point B;

[0019] Figure 5 This is a schematic diagram of the structure of the reagent bottle body in this utility model;

[0020] Figure 6 This utility model Figure 5 Enlarged structural diagram at point C;

[0021] Figure 7This is a schematic diagram of the pin and its surface in this utility model.

[0022] In the diagram: 1. Box body; 2. Box lid; 3. Cutter; 4. Positioning assembly; 41. Connecting rod; 42. Through hole; 43. Sliding sleeve; 44. Pin; 45. Compression spring; 46. Ring body; 47. Circular ring; 5. Reagent bottle body; 6. Round cap; 7. Pressure plate; 8. Connecting plate; 9. Rotating column; 10. Torsion spring; 11. Vertical column; 12. Horizontal plate; 13. Short rod; 14. Anti-slip rib. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-7 As shown, a well-sealed NK cell immunoassay kit includes a box body 1, a box lid 2, and a reagent bottle body 5. The box lid 2 is hinged to the box body 1. Positioning components 4 are provided on the surfaces of the box body 1 and the box lid 2. A cutter 3 is also attached to the surface of the box lid 2. A round cap 6 is provided on the top of the reagent bottle body 5. A pressure plate 7 is integrally provided on one side of the round cap 6. Several anti-slip ridges 14 are provided on the top of the pressure plate 7 to prevent slippage. A connecting plate 8 is fused to one side of the reagent bottle body 5. A rotating column 9 is rotatably connected to the surface of the connecting plate 8. A vertical column 11 is fixed to the surface of the rotating column 9, and the top of the vertical column 11 is fixed to the bottom of the connecting plate 8. The kit is integrally injection molded. A torsion spring 10 is fixed to one side of plate 8, and the other end of the torsion spring 10 is in contact with the vertical column 11. Horizontal plates 12 are also fixed to both sides of the reagent bottle body 5. Short rods 13 are fused to both sides of the round cap 6, and the other end of the short rods 13 is fused to the horizontal plates 12. Under the action of the short rods 13, the round cap 6 at the top of the reagent bottle body 5 is fixed to the horizontal plates 12, and the round cap 6 is fixed to the reagent bottle body 5. Thus, before the reagent bottle body 5 is used, the round cap 6 is fixed to prevent the inside from being exposed to the outside, increasing the sealing performance. The short rods 13, horizontal plates 12, pressure plates 7, round cap 6, and reagent bottle body 5 are all made of plastic. The short rods 13 are made of plastic and can be cut by the cutter 3.

[0025] The positioning assembly 4 includes a connecting rod 41, a through hole 42, and a sliding sleeve 43. The sliding sleeve 43 is bolted to the housing 1, and the connecting rod 41 is bolted to the housing cover 2. The through hole 42 is formed on the surface of the connecting rod 41, and the inner diameter of the through hole 42 is the same as the inner diameter of the sliding sleeve 43. A pin 44 is slidably connected to the inner wall of the sliding sleeve 43. A ring 47 is fixed to the surface of the pin 44. A compression spring 45 is fixed to one side of the ring 47, and the other end of the compression spring 45 is fixed to the sliding sleeve 43. Pulling the pin 44 causes it to move the ring 47 closer to the housing. Slide sleeve 43 and compress spring 45, then close box cover 2. At this time, through hole 42 and pin 44 are aligned. Then release pin 44. Under the action of compression spring 45 pushing ring 47 and pin 44, pin 44 enters through hole 42, fixing box cover 2 and box body 1 together. Pin 44 is made of plastic. One side of pin 44 is also fused with ring 46. Pass pin through ring 46 and pull pin 44 to move it, which provides a force point on the surface of pin 44.

[0026] During use, open the lid 2 and remove the reagent bottle body 5 from inside the box 1. Then, pick up the reagent bottle body 5, align the short rod 13 with the cutter 3, and cut the short rod 13. At this time, the round cover 6 is released from the horizontal plate 12. Then, press the pressure plate 7 with your fingers. The pressure plate 7 rotates under the action of the vertical column 11 and the rotating column 9, and drives the round cover 6 to rise. The torsion spring 10 is in a compressed state, which opens the reagent bottle body 5. The reagent bottle body 5 contains pre-filled liquid reagents such as culture medium, antibody solution, etc. Put the sample into the reagent bottle body 5. After sampling, release the pressure plate 7. Under the action of the torsion spring 10, the vertical column 11, the pressure plate 7 and the round cover 6 are reset, which prevents the staff from forgetting to seal the reagent bottle body 5 and causing the internal liquid to be contaminated.

[0027] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A well-sealed NK cell immunoassay kit, comprising a box body (1), a box cap (2), and a reagent bottle body (5), characterized in that, Also includes: Positioning components (4) are provided on the surfaces of the box body (1) and the box cover (2). A cutter (3) is also attached to the surface of the box cover (2). A round cover (6) is provided on the top of the reagent bottle body (5). A pressure plate (7) is integrally provided on one side of the round cover (6). A connecting plate (8) is fused to one side of the reagent bottle body (5). A rotating column (9) is rotatably connected to the surface of the connecting plate (8). A vertical column (11) is fixed to the surface of the rotating column (9), and the top of the vertical column (11) is fixed to the bottom of the connecting plate (8). A torsion spring (10) is fixed to one side of the connecting plate (8), and the other end of the torsion spring (10) is in contact with the vertical column (11). A horizontal plate (12) is also fixed on both sides of the reagent bottle body (5). Short rods (13) are fused to both sides of the round cap (6), and the other end of the short rods (13) is fused to the horizontal plate (12).

2. The NK cell immunoassay kit with good sealing properties according to claim 1, characterized in that: The positioning component (4) includes a connecting rod (41), a through hole (42), and a sliding sleeve (43). The sliding sleeve (43) is bolted to the box body (1), and the connecting rod (41) is bolted to the box cover (2). The through hole (42) is opened on the surface of the connecting rod (41). A pin (44) is slidably connected to the inner wall of the sliding sleeve (43). A ring (47) is fixed on the surface of the pin (44). A compression spring (45) is fixed on one side of the ring (47), and the other end of the compression spring (45) is fixed to the sliding sleeve (43).

3. The NK cell immunoassay kit with good sealing properties according to claim 2, characterized in that: A ring (46) is also fused to one side of the pin (44).

4. The NK cell immunoassay kit with good sealing properties according to claim 2, characterized in that: The inner diameter of the through hole (42) is the same as the inner diameter of the sliding sleeve (43).

5. The NK cell immunoassay kit with good sealing properties according to claim 1, characterized in that: The short rod (13) is made of plastic.

6. The NK cell immunoassay kit with good sealing properties according to claim 1, characterized in that: The top of the pressure plate (7) is provided with several anti-slip ridges (14).

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