Spatial multi-omics transfer device

Abstract

The utility model belongs to the technical field of space multi-omics, and discloses a space multi-omics transfer device which comprises a base, a hinge, a first limiting assembly and a second limiting assembly, the base is used for bearing a chip, the hinge is used for bearing a glass slide, the hinge is provided with a rotating part, and the rotating part is rotationally connected with the base; the chip and the glass slide are selectively arranged in a buckling manner; the first limiting assembly is arranged on the base and used for limiting the relative position of the chip and the base. The second limiting assembly is arranged on the hinge and used for limiting the relative position of the glass slide and the hinge. Thus, the chip and the glass slide can be supported by the base and the hinge respectively, the chip and the glass slide can be buckled by rotating the hinge, the tissue transfer efficiency is improved, the operation process is simplified, the space multi-group transfer device is compact and simple in structure, and the equipment cost can be reduced.

Description

Technical Field

[0001] This utility model relates to the field of space multi-omics technology, and in particular to a space multi-omics transfer device. Background Technology

[0002] Spatial transcriptomics is a rapidly developing field of biotechnology in recent years. It can analyze gene expression profiles while preserving the original spatial location information of tissue samples, thereby revealing the functional heterogeneity of cells in the tissue microenvironment.

[0003] Currently, spatial transcriptomics is developing towards high-resolution analysis and multi-omics joint analysis. For spatial multi-omics experiments, tissue pretreatment is usually required. If the tissue is directly attached to the chip and pretreated, the high temperature and acid-base reagent treatment during the pretreatment process may affect the stability of the nucleic acid sequence on the capture chip. Pretreatment of the tissue before transferring it to the chip can reduce this damage.

[0004] However, existing space multi-omics transfer devices are complex to operate and expensive, resulting in a high barrier to entry for experiments and hindering the widespread adoption of space transcriptomics technology. Utility Model Content

[0005] The purpose of this invention is to provide a space multi-omics transfer device to solve the problems of complex operation and high equipment cost in space multi-omics transfer setup.

[0006] To achieve this objective, the present invention adopts the following technical solution:

[0007] A spatial multi-omics transfer device includes: a base and a hinge, the base for supporting a chip, the hinge for supporting a glass slide, the hinge having a rotating part rotatably connected to the base to selectively engage the chip and the glass slide; a first limiting component disposed on the base for limiting the relative position of the chip and the base; and a second limiting component disposed on the hinge for limiting the relative position of the glass slide and the hinge.

[0008] Preferably, the base has a first limiting groove for limiting the position of the chip, and the hinge has a second limiting groove for limiting the position of the glass slide.

[0009] Preferably, the length direction of the first limiting groove is perpendicular to the length direction of the second limiting groove.

[0010] Preferably, one of the base and the hinge is provided with a connecting part, and the other is provided with a connecting block corresponding to the connecting part, and the connecting part and the connecting block are selectively engaged.

[0011] Preferably, the connecting portion is located at the end of the hinge opposite to the rotating portion.

[0012] Preferably, the first limiting component includes a first limiting block and a first latching block. The first limiting block is fixedly disposed on the base, and the first latching block is slidably disposed on the base. The first limiting block and the first latching block are respectively disposed on both sides of the chip. The second limiting component includes a second limiting block and a second latching block. The second limiting block is fixedly disposed on the hinge, and the second latching block is slidably disposed on the hinge. The second limiting block and the second latching block are respectively disposed on both sides of the glass slide.

[0013] Preferably, the first latching block is connected to a first spring so that the first latching block and the first limiting block clamp the chip; and / or, the second latching block is connected to a second spring so that the second latching block and the second limiting block clamp the glass slide.

[0014] Preferably, the base has a receiving groove corresponding to the positions of the second limiting block and the second snap-fit ​​block, and when the chip is attached to the glass slide, the second limiting block and the second snap-fit ​​block are located in the receiving groove.

[0015] Preferably, at least two sets of the first limiting components are provided at intervals along the length direction of the base; and / or, at least two sets of the second limiting components are provided at intervals along the length direction of the hinge.

[0016] Preferably, the hinge has an observation window, and when the chip and the glass slide are fastened together, the projection of the observation window coincides with the projection of the chip along the thickness direction of the base.

[0017] The beneficial effects of this utility model are:

[0018] A spatial multi-omics transfer device includes a base, a hinge, a first limiting component, and a second limiting component. The base is used to carry a chip, and the hinge is used to carry a glass slide. The hinge is provided with a rotating part, which is rotatably connected to the base so that the chip and the glass slide can be selectively engaged. The first limiting component is disposed on the base to limit the relative position between the chip and the base. The second limiting component is disposed on the hinge to limit the relative position between the glass slide and the hinge.

[0019] In this way, the base and hinge can support the chip and the slide respectively. By rotating the hinge, the slide can be placed on the chip and the tissue can be transferred, which improves the transfer efficiency and simplifies the operation process. Under the action of the first and second limiting components, the position of the chip and the slide is restricted, which prevents the chip and the slide from shifting or even falling off during the transfer process, thus improving the transfer effect. In addition, the spatial multi-omics transfer device has a compact structure and occupies a small volume, which can reduce the equipment cost. Attached Figure Description

[0020] Figure 1 This is a first structural schematic diagram of a spatial multi-omics transfer device in one embodiment of the present invention;

[0021] Figure 2 This is a schematic diagram of the second structure of a space multi-omics device in one embodiment of the present invention;

[0022] Figure 3 This is a schematic diagram of the third structure of a space multi-omics device in one embodiment of the present invention;

[0023] Figure 4 This is a first bottom view of a space multi-omics device in one embodiment of the present invention;

[0024] Figure 5 This is a top view of a space multi-omics device according to an embodiment of the present invention;

[0025] Figure 6 This is a second bottom view of a space multi-omics device in one embodiment of this utility model.

[0026] In the picture:

[0027] 1. Chip; 2. Glass slide; 3. Base; 31. First limiting groove; 32. Connecting block; 33. Receiving groove; 4. Hinge; 41. Rotating part; 42. Second limiting groove; 43. Connecting part; 44. Observation window; 5. First limiting component; 51. First limiting block; 52. First locking block; 6. Second limiting component; 61. Second limiting block; 62. Second locking block. Detailed Implementation

[0028] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.

[0029] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0030] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0031] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0032] See Figure 1 This utility model provides a spatial multi-omics transfer device, including a base 3, a hinge 4, a first limiting component 5, and a second limiting component 6. The base 3 is used to support a chip 1, and the hinge 4 is used to support a glass slide 2. The hinge 4 is provided with a rotating part 41, which is rotatably connected to the base 3 so that the chip 1 and the glass slide 2 can be selectively engaged. The first limiting component 5 is provided on the base 3 to limit the relative position of the chip 1 and the base 3. The second limiting component 6 is provided on the hinge 4 to limit the relative position of the glass slide 2 and the hinge 4.

[0033] In this embodiment, the length direction of the base 3 is parallel to the length direction of the chip 1, the length direction of the hinge 4 is parallel to the length direction of the slide 2, the chip 1 is secured to the base 3, and the slide 2 is secured to the hinge 4, so that when the hinge 4 rotates to abut against the base 3, the slide 2 and the chip 1 are engaged, which facilitates the transfer of tissue on the slide 2 to the chip 1.

[0034] Thus, the base 3 and the hinge 4 can support the chip 1 and the slide 2 respectively. By rotating the hinge 4, the chip 1 and the slide 2 can be engaged, which can improve the transfer efficiency and simplify the operation process. The first limiting component 5 and the second limiting component 6 can limit the position of the chip 1 and the slide 2, preventing the chip 1 and the slide 2 from shifting or even falling off during the operation, which would affect the transfer effect. In addition, the overall structure of the spatial multi-omics transfer device is simplified, reducing the equipment cost.

[0035] See Figure 2 In some embodiments, the base 3 is provided with a first limiting groove 31 for limiting the position of the chip 1, and the hinge 4 is provided with a second limiting groove 42 for limiting the position of the glass slide 2.

[0036] In this embodiment, the shape of the first limiting groove 31 is adapted to the shape of the chip 1, and the shape of the second limiting groove 42 is adapted to the shape of the glass slide 2, so that the chip 1 is locked in the first limiting groove 31 and the glass slide 2 is locked in the second limiting groove 42.

[0037] This improves the assembly efficiency of chip 1 and slide 2, helps align the tissue on slide 2 with the target position on chip 1, enhances the transfer effect, avoids experimental errors caused by positional misalignment of chip 1 or slide 2 during tissue transfer, and protects chip 1 and slide 2, reducing damage caused by improper installation. Chip 1 and slide 2 can be fastened together by rotating hinge 4, simplifying the operation process and improving transfer efficiency.

[0038] It is understandable that the positions of the first limiting groove 31 and the second limiting groove 42 can be adjusted according to actual needs, so that after the slide 2 and the chip 1 are fastened together, the tissue on the slide 2 can be transferred to the target position on the chip 1. No further details are provided here.

[0039] See Figure 2 In some embodiments, the length direction of the first limiting groove 31 is perpendicular to the length direction of the second limiting groove 42, that is, when the glass slide 2 abuts against the chip 1, the glass slide 2 is perpendicular to the chip 1.

[0040] In this embodiment, the axis of rotation of the rotating part 41 of the hinge 4 is parallel to the length direction of the base 3 (that is, the length direction of the chip 1), so that after the hinge 4 abuts against the base 3, the glass slide 2 and the chip 1 are arranged in a cross shape.

[0041] This facilitates the alignment of the tissue to be transferred on slide 2 with the target position on chip 1, improving the accuracy of tissue transfer, avoiding tissue transfer failure or experimental data deviation, improving the reliability and repeatability of the experiment, reducing the number of times the operator needs to adjust chip 1 and slide 2, and improving transfer efficiency.

[0042] See Figure 3 In some embodiments, one of the base 3 and the hinge 4 is provided with a connecting portion 43, and the other is provided with a connecting block 32 corresponding to the connecting portion 43. The connecting portion 43 and the connecting block 32 are selectively engaged. Further, in some embodiments, the connecting portion 43 is provided at the end of the hinge 4 opposite to the rotating portion 41.

[0043] In this embodiment, a connecting part 43 is provided. The connecting part 43 is rotatably disposed on the hinge 4. When the hinge 4 rotates to abut against the base 3, the connecting part 43 is fastened to the connecting block 32, which can limit the relative position of the hinge 4 and the base 3, so that the chip 1 and the glass slide 2 are stably and continuously abutted, so as to facilitate the transfer of tissue on the glass slide 2 to the chip 1.

[0044] Thus, the connection between the connecting part 43 and the connecting block 32 can improve the connection stability between the hinge 4 and the base 3, avoid the transfer effect being affected by the loosening of the hinge 4 during the transfer process, simplify the operation process, reduce human error caused by complex operation steps, improve the success rate and efficiency of tissue transfer, and enable the slide 2 and the chip 1 to be accurately connected, thereby improving the effect of tissue transfer.

[0045] It is understandable that multiple connecting parts 43 can be provided. The hinge 4 and the base 3 can be connected by connecting multiple connecting parts 43 with their corresponding connecting blocks 32. The number and setting position of the connecting parts 43 can be adjusted according to actual needs, and will not be listed in detail here.

[0046] See Figure 4 and Figure 5 In some embodiments, the first limiting component 5 includes a first limiting block 51 and a first latching block 52. The first limiting block 51 is fixedly disposed on the base 3, and the first latching block 52 is slidably disposed on the base 3. The first limiting block 51 and the first latching block 52 are respectively disposed on both sides of the chip 1. The second limiting component 6 includes a second limiting block 61 and a second latching block 62. The second limiting block 61 is fixedly disposed on the hinge 4, and the second latching block 62 is slidably disposed on the hinge 4. The second limiting block 61 and the second latching block 62 are respectively disposed on both sides of the glass slide 2.

[0047] In this embodiment, the first limiting block 51 and the first latching block 52 respectively clamp the two sides of the chip 1 in the width direction. The sliding direction of the first latching block 52 is parallel to the width direction of the chip 1. The second limiting block 61 and the second latching block 62 respectively clamp the two sides of the glass slide 2 in the width direction. The sliding direction of the second latching block 62 is parallel to the width direction of the glass slide 2.

[0048] In this way, when assembling chip 1, chip 1 can first abut against the first limiting block 51, and the first locking block 52 can be slid until it abuts against chip 1, avoiding the difficulty of assembling chip 1 due to the distance between the first limiting block 51 and the first locking block 52 being too close. Similarly, the cooperation of the second limiting block 61 and the second locking block 62 can stably clamp the slide 2, improve the stability of chip 1 and slide 2, and improve the efficiency and success rate of tissue transfer.

[0049] See Figure 4 and Figure 5 In some embodiments, the first latching block 52 is connected to a first spring (not shown in the figure) so that the first latching block 52 and the first limiting block 51 clamp the chip 1, and the second latching block 62 is connected to a second spring (not shown in the figure) so that the second latching block 62 and the second limiting block 61 clamp the glass slide 2.

[0050] In this embodiment, the end of the first spring away from the first latching block 52 is fixedly connected to the base 3, and the end of the second spring away from the second latching block 62 is fixedly connected to the hinge 4.

[0051] It should be noted that when the first locking block 52 is pulled to move away from the first limiting block 51, the first spring is compressed, and the chip 1 is locked in the first limiting groove 31. After the first locking block 52 is released, the first spring drives the first locking block 52 to move towards the first limiting block 51, thereby locking the chip 1. The assembly of the glass slide 2 is similar.

[0052] Thus, by setting the first spring and the second spring, the first locking block 52 can automatically engage with the chip 1, and the second locking block 62 can automatically engage with the glass slide 2, improving assembly efficiency. Furthermore, it restricts the relative position of the chip 1 on the base 3 and the relative position of the glass slide 2 on the hinge 4, making it less likely for the glass slide 2 to fall out of the second limiting groove 42 during the rotation of the hinge 4 and affect the transfer effect, thereby improving transfer efficiency and simplifying the operation process. The chip 1 and the glass slide 2 can be limited with a simple structure, reducing equipment costs.

[0053] See Figure 2 In some embodiments, a receiving groove 33 is provided on the base 3 at the position corresponding to the second limiting block 61 and the second snap-fit ​​block 62. When the chip 1 is attached to the glass slide 2, the second limiting block 61 and the second snap-fit ​​block 62 are located in the receiving groove 33.

[0054] In this embodiment, multiple receiving slots 33 are provided, and the multiple receiving slots 33 are respectively provided on both sides of the first limiting slot 31 so that the hinge 4 and the base 3 can be tightly abutted. The first limiting block 51 and the first snap-fit ​​block 52 are respectively provided on both sides of the hinge 4 to avoid interference with the hinge 4.

[0055] In this way, interference between the second limiting block 61 and the second locking block 62 and the base 3 can be avoided when the hinge 4 rotates to engage with the base 3, thereby affecting the engagement effect of the chip 1 and the glass slide 2. The receiving groove 33 can provide space to accommodate the second limiting block 61 and the second locking block 62, so as to achieve a tight contact between the hinge 4 and the base 3 and improve the transfer effect.

[0056] See Figure 1 In some embodiments, at least two sets of first limiting components 5 are provided at intervals along the length direction of the base 3, and at least two sets of second limiting components 6 are provided at intervals along the length direction of the hinge 4.

[0057] In this embodiment, the first limiting component 5 and the second limiting component 6 are each provided in two sets, which are used to clamp the two ends of the chip 1 and the two ends of the glass slide 2.

[0058] In this way, both ends of the chip 1 and both ends of the glass slide 2 can be stably clamped, making the force on the chip 1 and the glass slide 2 more uniform, improving the clamping stability, avoiding displacement or excessive local force that would affect the clamping effect, and enabling the chip 1 and the glass slide 2 to maintain a stable connection during the transfer process, improving the transfer effect, simplifying the operation process, and improving the transfer efficiency.

[0059] It is understandable that the number of the first limiting component 5 and the second limiting component 6 can be equal or unequal. The number of the first limiting component 5 and the second limiting component 6 can be adjusted according to actual needs, as long as it can limit the position of the chip 1 and the glass slide 2. No further details are provided here.

[0060] See Figure 1 and Figure 2 In some embodiments, the hinge 4 has an observation window 44. When the chip 1 and the glass slide 2 are fastened together, the projection of the observation window 44 along the thickness direction of the base 3 coincides with the projection of the chip 1.

[0061] In this embodiment, the observation window 44 is disposed through the hinge 4, and the cross-sectional area of ​​the observation window 44 is smaller than the cross-sectional area of ​​the first limiting groove 31.

[0062] Thus, by setting the observation window 44, the operator can easily observe whether the tissue on the slide 2 is aligned with the target position on the chip 1 when rotating the hinge 4, so as to adjust the position of the slide 2 in time. The spatial multi-omics transfer device has a compact structure and small size, and can be compatible with scanning microscopes, allowing the scanning microscope to observe the tissue state through the observation window 44, making the application scenarios of the spatial multi-omics transfer device more diverse.

[0063] Understandably, see Figure 5 and Figure 6An observation window 44 can also be opened on the base 3. The position of the observation window 44 can be adjusted according to actual needs, which will not be elaborated here.

[0064] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A space multi-omics transfer device, characterized in that, include: The base (3) and hinge (4) are provided. The base (3) is used to support the chip (1) and the hinge (4) is used to support the glass slide (2). The hinge (4) is provided with a rotating part (41). The rotating part (41) is rotatably connected to the base (3) so that the chip (1) and the glass slide (2) can be selectively engaged. A first limiting component (5) is disposed on the base (3) to limit the relative position of the chip (1) and the base (3); The second limiting component (6) is disposed on the hinge (4) and is used to limit the relative position of the slide (2) and the hinge (4).

2. The space multi-omics transfer device according to claim 1, characterized in that, The base (3) has a first limiting groove (31) for limiting the position of the chip (1), and the hinge (4) has a second limiting groove (42) for limiting the position of the glass slide (2).

3. The space multi-omics transfer device according to claim 2, characterized in that, The length direction of the first limiting groove (31) is perpendicular to the length direction of the second limiting groove (42).

4. The space multi-omics transfer device according to claim 1, characterized in that, One of the base (3) and the hinge (4) is provided with a connecting part (43), and the other is provided with a connecting block (32) corresponding to the connecting part (43). The connecting part (43) and the connecting block (32) can be selectively engaged.

5. The space multi-omics transfer device according to claim 4, characterized in that, The connecting part (43) is located at one end of the hinge (4) away from the rotating part (41).

6. The space multi-omics transfer device according to claim 1, characterized in that, The first limiting component (5) includes a first limiting block (51) and a first latching block (52). The first limiting block (51) is fixedly disposed on the base (3), and the first latching block (52) is slidably disposed on the base (3). The first limiting block (51) and the first latching block (52) are respectively disposed on both sides of the chip (1). The second limiting component (6) includes a second limiting block (61) and a second latching block (62). The second limiting block (61) is fixedly disposed on the hinge (4), and the second latching block (62) is slidably disposed on the hinge (4). The second limiting block (61) and the second latching block (62) are respectively disposed on both sides of the glass slide (2).

7. The space multi-omics transfer device according to claim 6, characterized in that, The first latching block (52) is connected to a first spring so that the first latching block (52) and the first limiting block (51) clamp the chip (1); and / or, the second latching block (62) is connected to a second spring so that the second latching block (62) and the second limiting block (61) clamp the glass slide (2).

8. The space multi-omics transfer device according to claim 6, characterized in that, The base (3) has a receiving groove (33) at the position corresponding to the second limiting block (61) and the second snap-fit ​​block (62). When the chip (1) is attached to the glass slide (2), the second limiting block (61) and the second snap-fit ​​block (62) are located in the receiving groove (33).

9. The space multi-omics transfer device according to any one of claims 1-8, characterized in that, The first limiting component (5) is provided in at least two sets at intervals along the length direction of the base (3); and / or, the second limiting component (6) is provided in at least two sets at intervals along the length direction of the hinge (4).

10. The space multi-omics transfer device according to any one of claims 1-8, characterized in that, The hinge (4) has an observation window (44). When the chip (1) and the glass slide (2) are fastened together, the projection of the observation window (44) along the thickness direction of the base (3) coincides with the projection of the chip (1).

Images