Second-generation technology targeted sequencing device
By introducing an automated combination of slide rails and motorized telescopic rods into the second-generation targeted sequencing device, the problem of low mixing efficiency in existing devices has been solved, achieving uniform mixing and automated operation of liquids in test tubes, thus improving sequencing efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZHANGJIAGANG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
- Filing Date
- 2025-05-22
- Publication Date
- 2026-05-12
AI Technical Summary
Existing second-generation targeted sequencing devices have poor mixing effects through lateral shaking, are time-consuming, and require manual operation, resulting in low sequencing efficiency.
The system employs a combination design of a first linear slide rail, an electric telescopic rod, a clamping part, a support assembly, a limiting assembly, and a lifting assembly to achieve automatic loading and unloading of test tubes and compound shaking mixing, reducing dead zones and improving mixing efficiency.
This method achieves uniform mixing of liquids within the test tube, shortens mixing time, improves the efficiency of targeted sequencing, avoids test tube damage, and reduces costs.
Smart Images
Figure CN224227065U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of sequencing device technology, and in particular to a second-generation targeted sequencing device. Background Technology
[0002] Next-generation (NGS) targeted sequencing is an advanced DNA sequencing method that combines the advantages of high-throughput sequencing and targeted capture technologies. This method enables deep sequencing of specific genomic regions, allowing for rapid and accurate acquisition of their genetic information. By designing specific probes or utilizing capture techniques, NGS targeted sequencing can selectively capture and sequence genes or regions of interest, avoiding the large amounts of irrelevant data and costs associated with whole-genome sequencing. This sequencing method is widely used in genomics research, genetic disease diagnosis, and drug development, providing strong technical support for scientific research and clinical applications. In the NGS targeted sequencing process, a DNA library is mixed with a hybridization solution containing targeted probes, and the hybridization reaction is carried out under appropriate temperature and time conditions. The probes specifically bind to the target regions in the DNA library, thus achieving targeted capture.
[0003] The Chinese patent with authorized publication number CN215028270U uses the reciprocating motion of a slider to shake the test tubes on the test tube rack to achieve mixing. However, the mixing effect of lateral shaking alone is not good, it takes a long time, and it requires manual removal and insertion of test tubes, resulting in low efficiency of targeted sequencing. Utility Model Content
[0004] The purpose of this invention is to solve the aforementioned problems existing in the prior art by proposing a second-generation targeted sequencing device.
[0005] To achieve the above objectives, the present invention adopts the following technical solution: a second-generation targeted sequencing device, comprising a device housing, wherein a first linear slide rail is fixedly disposed at the upper end of the device housing, a first electric telescopic rod is fixedly disposed at the lower end of the first linear slide rail, a clamping part is fixedly disposed at the lower end of the first electric telescopic rod, an installation box and an installation housing are fixedly connected to the lower end of the device housing, a support component and a limiting component are disposed inside the installation box, and two sets of the support component and the limiting component are symmetrically disposed thereon, a fourth electric telescopic rod is fixedly connected to the inner wall of one side of the installation box, a push plate is fixedly connected to one end of the fourth electric telescopic rod, and the push plate is connected to both sets of the limiting components;
[0006] A rubber plate is fixedly connected to the lower end of the installation box. A limiting hole plate is fixed to the inner wall of the installation box above the rubber plate. A movable plate is contacted at the upper end of the limiting hole plate, and five sets of movable plates are arranged at intervals. All five sets of movable plates are slidably connected to the inner wall of the installation box. Through holes with matching positions and sizes are opened on the movable plates and the limiting hole plate. Lifting components are provided on both sides of the inner wall of the installation box.
[0007] Preferably, the lifting assembly includes a second linear slide fixedly connected to the inner wall of one side of the mounting box and a fifth electric telescopic rod fixedly connected to the upper end of the second linear slide.
[0008] Preferably, the support assembly includes a second electric telescopic rod fixedly connected to the lower end of the mounting box, a support plate fixedly connected to the upper end of the second electric telescopic rod, and a U-shaped mounting plate slidably connected to the upper end of the support plate.
[0009] Preferably, the lower end of the U-shaped mounting plate is fixedly connected to an embedded rod, and a sliding strip is slidably arranged inside the lower end of the mounting box. One end of the sliding strip is fixedly connected to one side of the push plate, and the lower end of the embedded rod is movably engaged with the upper end of the sliding strip.
[0010] Preferably, the limiting component includes two connecting rods rotatably connected to both sides of the U-shaped mounting plate, and a fixing plate is fixedly connected to one end of each of the two sets of connecting rods. A first limiting strip is slidably arranged between the two sets of fixing plates, and a second limiting strip is fixedly arranged between the two sets of fixing plates. A motor is fixedly connected to one side of the U-shaped mounting plate, and the output end of the motor is fixedly connected to one set of the connecting rods.
[0011] Preferably, a third electric telescopic rod is embedded and fixedly connected to one side of the second limiting strip, and the other end of the third electric telescopic rod is embedded and fixedly connected to one side of the first limiting strip, and two sets of the third electric telescopic rod are provided.
[0012] Preferably, the first and second limiting strips are provided with a plurality of through grooves, and anti-collision rubber strips are fixedly provided inside the through grooves.
[0013] Compared with existing technologies, the advantages of this utility model are:
[0014] 1. In this application, by setting up a first linear slide rail, a first electric telescopic rod and a clamping part, automatic loading and unloading of test tubes can be realized without manual handling, which improves the efficiency of targeted sequencing. By setting up a second linear slide, a fifth electric telescopic rod and a movable plate, the lifting of the test tubes avoids the situation where test tubes next to the test tubes to be moved are damaged by impact.
[0015] 2. In this application, the combination of support components, limiting components, a fourth electric telescopic rod, and a push plate, along with a composite shaking method, reduces dead zones during the mixing process, resulting in more uniform mixing of the liquid in the test tube. This also shortens the mixing time and improves the efficiency of targeted sequencing. The alternating use of the two sets of limiting components effectively enhances mixing efficiency. Furthermore, the separable design of the embedded rod and sliding bar eliminates the need for multiple fourth electric telescopic rods and push plates, enabling lateral shaking of the two sets of limiting components and reducing costs. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the overall structure of a second-generation targeted sequencing device proposed in this invention;
[0017] Figure 2 This is a schematic diagram of the internal structure of the device housing of a second-generation targeted sequencing device proposed in this invention;
[0018] Figure 3 This is a schematic diagram of the lifting component, limiting plate, movable plate, and rubber plate of a second-generation targeted sequencing device proposed in this invention.
[0019] Figure 4 This is a schematic plan view of the support components, fourth electric telescopic rod, and push plate structure of a second-generation targeted sequencing device proposed in this invention.
[0020] Figure 5 This is a schematic diagram of the limiting component structure of a second-generation targeted sequencing device proposed in this invention;
[0021] Figure 6 This is a schematic diagram showing the structural breakdown of the limiting component of a second-generation targeted sequencing device proposed in this invention.
[0022] In the diagram: 1. Equipment housing; 2. Mounting box; 3. First linear slide rail; 4. First electric telescopic rod; 5. Clamping part; 6. Mounting box; 7. Support assembly; 71. U-shaped mounting plate; 72. Support plate; 73. Second electric telescopic rod; 74. Embedded rod; 75. Sliding strip; 8. Lifting assembly; 81. Second linear slide table; 82. Fifth electric telescopic rod; 9. Limiting hole plate; 10. Movable plate; 11. Rubber plate; 12. Limiting assembly; 121. Connecting rod; 122. Fixing plate; 123. First limiting strip; 124. Motor; 125. Anti-collision rubber strip; 126. Second limiting strip; 127. Third electric telescopic rod; 13. Fourth electric telescopic rod; 14. Push plate. Detailed Implementation
[0023] The following embodiments are for illustrative purposes only and are not intended to limit the scope of this invention.
[0024] Example
[0025] Reference Figures 1 to 6 A second-generation targeted sequencing device includes a device housing 1. A first linear slide rail 3 is fixedly mounted on the upper part of the device housing 1. A first electrically operated telescopic rod 4 is fixedly mounted on the lower part of the first linear slide rail 3. A clamping part 5 is fixedly mounted on the lower part of the first electrically operated telescopic rod 4. Clamping multiple test tubes via the clamping part 5 is a known prior art, and those skilled in the art can conceive of the specific structure. An installation box 6 and an installation housing 2 are fixedly connected to the lower part of the device housing 1. The installation box 6 contains a support component 7 and a limiting component 12, with two sets of each symmetrically arranged. A fourth electrically operated telescopic rod 13 is fixedly connected to the inner wall of one side of the installation box 6. The fourth electric telescopic rod 13 is fixedly connected to a push plate 14 at one end, and the push plate 14 is connected to both sets of limiting components 12. The position of the first electric telescopic rod 4 and the clamping part 5 can be adjusted by the first linear slide rail 3. The height of the clamping part 5 can be adjusted by the first electric telescopic rod 4, so that the test tube inside the mounting box 2 can be clamped to the position of the limiting component 12. The support component 7 is used to install the limiting component 12. The limiting component 12 is used to place the test tube and uniformly mix the DNA library inside the test tube with the hybridization solution containing the target probe. The fourth electric telescopic rod 13 and the push plate 14 are used to drive the test tube placed on the limiting component 12 to move back and forth to improve the mixing effect.
[0026] A rubber plate 11 is fixedly connected to the lower end of the interior of the mounting box 2. A limiting hole plate 9 is fixed to the inner wall of the mounting box 2 above the rubber plate 11. A movable plate 10 is contacted at the upper end of the limiting hole plate 9, and five sets of movable plates 10 are arranged at intervals. All five sets of movable plates 10 are slidably connected to the inner wall of the mounting box 2. Through holes with matching positions and sizes are opened on the movable plates 10 and the limiting hole plate 9. Lifting components 8 are provided on both sides of the inner wall of the mounting box 2. The lifting components 8 are used to control the height of the movable plates 10. By lifting the movable plates 10, the height of the test tubes placed through the limiting hole plate 9 and the movable plates 10 and embedded in the rubber plate 11 can also be raised. By raising the test tubes, the test tubes to be moved are higher than other test tubes, so that the test tubes will not affect other test tubes when they are clamped and picked up. The limiting hole plate 9, the movable plate 10 and the rubber plate 11 are all used to limit the test tubes.
[0027] The lifting assembly 8 includes a second linear slide 81 fixedly connected to the inner wall of one side of the mounting box 2 and a fifth electric telescopic rod 82 fixedly connected to the upper end of the second linear slide 81. The fifth electric telescopic rod 82 can be moved to the position of the movable plate 10 below the test tube to be moved by the second linear slide 81. Then, the movable plate 10 is lifted by the fifth electric telescopic rod 82. Since the size of the through hole on the movable plate 10 is smaller than the size of the test tube cap, a row of test tubes can be lifted upwards. Then, the test tubes can be clamped by the clamping part 5, which can prevent the test tubes next to the test tube to be moved from being damaged by impact when clamping the test tubes and taking them out after mixing.
[0028] The support assembly 7 includes a second electric telescopic rod 73 fixedly connected to the lower end of the mounting box 6. A support plate 72 is fixedly connected to the upper end of the second electric telescopic rod 73. A U-shaped mounting plate 71 is slidably connected to the upper end of the support plate 72. The second electric telescopic rod 73 and the support plate 72 are used to support the U-shaped mounting plate 71.
[0029] The lower end of the U-shaped mounting plate 71 is fixedly connected to an embedded rod 74. A sliding strip 75 is slidably provided inside the lower end of the mounting box 6, and one end of the sliding strip 75 is fixedly connected to one side of the push plate 14. The lower end of the embedded rod 74 is movably engaged with the upper end of the sliding strip 75. The fourth electric telescopic rod 13 can push and pull the embedded rod 74 through the push plate 14 and the sliding strip 75, so that the U-shaped mounting plate 71 can slide back and forth on the support plate 72. The height of the U-shaped mounting plate 71 can be controlled by the second electric telescopic rod 73, thereby controlling the connection between the embedded rod 74 and the sliding strip 75.
[0030] The limiting assembly 12 includes two connecting rods 121 rotatably connected to both sides of the U-shaped mounting plate 71. Each of the two sets of connecting rods 121 has a fixed plate 122 fixedly connected to one end of each set of connecting rods 121. A first limiting strip 123 is slidably arranged between the two sets of fixed plates 122. A second limiting strip 126 is fixedly arranged between the two sets of fixed plates 122. A motor 124 is fixedly connected to one side of the U-shaped mounting plate 71, and the output end of the motor 124 is fixedly connected to one set of connecting rods 121. The motor 124 can drive the connecting rods 121, the fixed plate 122 and the first limiting strip 123 to swing back and forth.
[0031] A third electric telescopic rod 127 is embedded and fixedly connected to one side of the second limiting strip 126. The other end of the third electric telescopic rod 127 is embedded and fixedly connected to one side of the first limiting strip 123. Two sets of the third electric telescopic rod 127 are provided. The position of the two sets of first limiting strips 123 can be controlled by the third electric telescopic rod 127, so that the first limiting strip 123 and the second limiting strip 126 can clamp and release the test tube.
[0032] The first limiting strip 123 and the second limiting strip 126 are provided with multiple through grooves, and anti-collision rubber strips 125 are fixedly provided inside the through grooves. The anti-collision rubber strips 125 are used to increase friction so that the test tube will not slip when shaking and moving.
[0033] In use, this invention first places test tubes containing a DNA library and a hybridization solution containing a target probe onto the limiting plate 9, the movable plate 10, and the rubber plate 11. Then, the fifth electric telescopic rod 82 is moved below the movable plate 10 via the second linear slide 81. The fifth electric telescopic rod 82 then lifts both the movable plate 10 and the test tubes upwards. Subsequently, the first linear slide rail 3, the first electric telescopic rod 4, and the clamping part 5 work together to clamp a row of test tubes above the first limiting bar 123. This lifting of the test tubes prevents damage to test tubes next to the ones to be moved from impact. Finally, the third electric telescopic rod 127 moves the first limiting bar 123... 23 separates from the second limiting strip 126, and then the first electric telescopic rod 4 moves the test tube to the inside of the anti-collision rubber strip 125. Subsequently, the third electric telescopic rod 127 clamps and fixes multiple sets of test tubes with the first limiting strip 123 and the second limiting strip 126. Then, the first linear slide rail 3, the first electric telescopic rod 4, and the clamping part 5 repeat the clamping process to place the test tube onto another set of limiting components 12. During this process, the second electric telescopic rod 73 causes the lower end of the embedding rod 74 below the test tube to embed into the upper end of the sliding strip 75. Then, the motor 124 causes the test tube to rotate back and forth, and at the same time, the fourth electric telescopic rod 13 can push the embedding rod 74 to move horizontally, thereby making the U-shaped safety... The mounting plate 71 can cause the first limiting bar 123 and the second limiting bar 126 to sway laterally, causing multiple sets of test tubes to also sway laterally. The reciprocating rotation generates eddies and turbulence inside the test tubes, mixing the liquids. The addition of lateral swaying, through this combined swaying method, reduces dead zones during mixing, resulting in more uniform mixing of the liquids within the test tubes. It also shortens the mixing time and improves efficiency. After mixing, the clamping part 5 places the test tubes to be mixed between another set of first limiting bars 123 and second limiting bars 126. Then, the clamping part 5 moves the mixed test tubes back to their original position inside the mounting box 2. During this movement, the clamping part 5... The second electric telescopic rod 73 causes the lower end of the embedding rod 74 below the test tube to embed into the upper end of the sliding strip 75, repeatedly performing compound shaking, which can realize the automatic loading and unloading of test tubes without manual handling. At the same time, the alternating use of the two sets of limiting components 12 can effectively improve the mixing efficiency. Through the separable design of the embedding rod 74 and the sliding strip 75, the lateral shaking of the two sets of limiting components 12 can be realized without setting multiple fourth electric telescopic rods 13 and push plates 14. After mixing, the staff can take out the test tube from the installation box 2. When taking it out, the fifth electric telescopic rod 82 lifts both the movable plate 10 and the test tube upwards, which can prevent the test tube from being damaged by collision with adjacent test tubes during handling.
[0034] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A second-generation targeted sequencing device, comprising a device housing (1), characterized in that, The upper part of the equipment housing (1) is fixedly provided with a first linear slide rail (3), the lower part of the first linear slide rail (3) is fixedly provided with a first electric telescopic rod (4), the lower part of the first electric telescopic rod (4) is fixedly provided with a clamping part (5), the lower part of the equipment housing (1) is fixedly connected with an installation box (6) and an installation box (2), the installation box (6) is provided with a support component (7) and a limiting component (12), and the support component (7) and the limiting component (12) are symmetrically arranged in two sets. The inner wall of one side of the installation box (6) is fixedly connected with a fourth electric telescopic rod (13), one end of the fourth electric telescopic rod (13) is fixedly connected with a push plate (14), and the push plate (14) is connected to both sets of the limiting components (12). A rubber plate (11) is fixedly connected to the lower end of the installation box (2). A limiting hole plate (9) fixed on the inner wall of the installation box (2) is provided above the rubber plate (11). A movable plate (10) is provided at the upper end of the limiting hole plate (9). Five sets of movable plates (10) are arranged at intervals. All five sets of movable plates (10) are slidably connected to the inner wall of the installation box (2). Through holes with matching positions and sizes are opened on the movable plates (10) and the limiting hole plate (9). Lifting components (8) are provided on both sides of the inner wall of the installation box (2).
2. The second-generation targeted sequencing device according to claim 1, characterized in that, The lifting assembly (8) includes a second linear slide (81) fixedly connected to the inner wall of one side of the mounting box (2) and a fifth electric telescopic rod (82) fixedly connected to the upper end of the second linear slide (81).
3. The second-generation targeted sequencing device according to claim 1, characterized in that, The support assembly (7) includes a second electric telescopic rod (73) fixedly connected to the lower end of the mounting box (6), a support plate (72) fixedly connected to the upper end of the second electric telescopic rod (73), and a U-shaped mounting plate (71) slidably connected to the upper end of the support plate (72).
4. The second-generation targeted sequencing device according to claim 3, characterized in that, The lower end of the U-shaped mounting plate (71) is fixedly connected to an embedded rod (74), and a sliding strip (75) is slidably provided inside the lower end of the mounting box (6). One end of the sliding strip (75) is fixedly connected to one side of the push plate (14), and the lower end of the embedded rod (74) is movably engaged with the upper end of the sliding strip (75).
5. The second-generation targeted sequencing device according to claim 3, characterized in that, The limiting component (12) includes two connecting rods (121) that are rotatably connected to both sides of the U-shaped mounting plate (71). Each of the two sets of connecting rods (121) has a fixed plate (122) fixedly connected to one end of each set of connecting rods (121). A first limiting strip (123) is slidably arranged between the two sets of fixed plates (122). A second limiting strip (126) is fixedly arranged between the two sets of fixed plates (122). A motor (124) is fixedly connected to one side of the U-shaped mounting plate (71), and the output end of the motor (124) is fixedly connected to one set of connecting rods (121).
6. The second-generation targeted sequencing device according to claim 5, characterized in that, The second limiting strip (126) has a third electric telescopic rod (127) embedded and fixedly connected on one side. The other end of the third electric telescopic rod (127) is embedded and fixedly connected to the first limiting strip (123) on one side, and two sets of the third electric telescopic rod (127) are provided.
7. The second-generation targeted sequencing device according to claim 6, characterized in that, The first limiting strip (123) and the second limiting strip (126) are provided with multiple through grooves, and anti-collision rubber strips (125) are fixedly provided inside the through grooves.