Constant-temperature activation device for enzyme liquid

Abstract

The utility model relates to the technical field of biotechnology, in particular to an enzyme liquid constant-temperature activation device, which not only enables water to keep constant temperature through a circulating pump and a heater, omits the trouble of frequent water replenishment and avoids the waste of energy and water resources, but also facilitates the fixation of a centrifugal tube and improves the use flexibility of the device. Comprising an activation box; the device further comprises a heating mechanism, a stirring mechanism, a lifting mechanism and a fixing mechanism, the heating mechanism is installed on the activation box and used for heating water, the stirring mechanism is installed on the activation box and used for stirring enzyme liquid, the lifting mechanism is installed on the activation box and used for driving the fixing mechanism to ascend and descend, and the fixing mechanism is installed on the lifting mechanism and used for supporting the centrifugal tube.

Description

Technical Field

[0001] This utility model relates to the technical field of biotechnology, and in particular to an enzyme solution constant temperature activation device. Background Technology

[0002] The production and application of enzymes have a history of more than 80 years both domestically and internationally. In the 1980s, bioengineering, as an emerging high technology, developed rapidly in my country, and the fields of enzyme manufacturing and application gradually expanded. Now, enzyme treatment technology is recognized as a green production process that meets environmental protection requirements.

[0003] Existing enzyme solution isothermal activation devices, such as the isothermal device for enzyme preservation disclosed in utility model patent application number 202420484359.X, mainly include a shell, a container for holding the enzyme disposed on the shell, and an insulation layer for heat preservation. A phase change heat-absorbing layer for absorbing and releasing latent heat is also provided between the container and the insulation layer. In use, after the enzyme is stored in the container, while the insulation layer keeps the container warm to prevent heat exchange between the container and the external environment, the latent heat can also be absorbed and released through the phase change heat-absorbing layer disposed in the shell mounting layer.

[0004] However, most existing activation devices maintain a constant temperature environment by replenishing water, which makes it difficult to respond to temperature changes in a timely manner. The residual heat in the water is not utilized and is wasted. Moreover, most existing activation devices are single-pass designs, which cannot flexibly fix small containers (such as centrifuge tubes). Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides an enzyme solution constant temperature activation device that not only keeps the water at a constant temperature through a circulating pump and heater, eliminating the trouble of frequent water replenishment and avoiding the waste of energy and water resources, but also facilitates the fixing of centrifuge tubes and improves the flexibility of the device.

[0006] This utility model discloses an enzyme solution constant temperature activation device, including an activation chamber; it also includes a heating mechanism, a stirring mechanism, a lifting mechanism, and a fixing mechanism. The heating mechanism is installed on the activation chamber and heats the water; the stirring mechanism is installed on the activation chamber and stirs the enzyme solution; the lifting mechanism is installed on the activation chamber and drives the fixing mechanism to rise and fall; the fixing mechanism is installed on the lifting mechanism and supports the centrifuge tube. When activating a large amount of enzyme solution, the enzyme solution is directly added to the activation chamber. The heating mechanism maintains the temperature required for enzyme solution activation, while the stirring mechanism stirs the enzyme solution to ensure uniform heating. When activating the enzyme solution in the centrifuge tube, the centrifuge tube is placed directly on the fixing mechanism. The lifting mechanism adjusts the height of the centrifuge tube, and the stirring mechanism drives the fixing mechanism and the centrifuge tube to rotate to ensure uniform heating.

[0007] Preferably, the activation box includes an insulated box, a cylindrical body, a support, two sets of first hydraulic cylinders, and a sealing cover. The bottom of the insulated box is connected to the ground, the cylindrical body is installed inside the insulated box, and a sandwich is formed between the cylindrical body and the insulated box. The sandwich is connected to a water supply pipe, and a cavity is provided inside the cylindrical body. The bottom of the support is connected to the top of the insulated box, and both sets of first hydraulic cylinders are installed on the support. The top of the sealing cover is connected to the bottom of the two sets of first hydraulic cylinders. The water supply pipe delivers water to the sandwich, the heating mechanism heats the water to a specified temperature, the insulated box keeps the water warm, and then the operator adds the enzyme solution into the cavity of the cylindrical body. The two sets of first hydraulic cylinders push the sealing cover down to seal the cavity, facilitating the activation of the enzyme solution.

[0008] Preferably, the heating mechanism includes a heater, a circulating pump, a pumping pipe, and a supply pipe. The heater is installed inside the interlayer, the circulating pump is installed on the insulation box, the pumping pipe is installed on the circulating pump and communicates with the bottom of the interlayer, and the supply pipe is installed on the circulating pump and communicates with the top of the interlayer. The heater heats the water inside the interlayer, the circulating pump is started, and the circulating pump draws hot water from the bottom of the interlayer through the pumping pipe and delivers the hot water to the top of the interlayer through the supply pipe, so that the water inside the interlayer is heated evenly and kept at the same temperature.

[0009] Preferably, the stirring mechanism includes a motor, a reducer, a rotating shaft, stirring blades, and a positioning strip. The bottom end of the motor is connected to the top end of the sealing cover, the bottom end of the reducer is connected to the top end of the sealing cover, the rotating shaft is rotatably mounted on the sealing cover and longitudinally connected to the reducer, the stirring blades are mounted on the rotating shaft, and the positioning strip is mounted on the rotating shaft. When the operator starts the motor, the motor drives the rotating shaft to rotate through the reducer, and the rotating shaft drives the stirring blades to rotate. The stirring blades stir the enzyme solution to ensure uniform heating, and the rotating shaft can easily drive the fixing mechanism to rotate through the positioning strip.

[0010] Preferably, the device further includes three sets of first thermometers installed inside the cavity of the cylinder, with the three sets of first thermometers distributed at different heights within the cavity; by setting three sets of first thermometers, it is convenient to detect the temperature of the enzyme solution. When a large temperature difference is detected between the upper and lower layers of the enzyme solution, the water flow rate of the circulating pump is increased by an electrical signal to ensure uniform heating at different heights of the cylinder. At the same time, the speed of the motor is increased by an electrical signal to ensure uniform mixing of the enzyme solution.

[0011] Preferably, the lifting mechanism includes two sets of second hydraulic cylinders, an annular mounting bracket, and a second thermometer. Both sets of second hydraulic cylinders are mounted on the sealing cover. The top of the annular mounting bracket is connected to the bottom of the two sets of second hydraulic cylinders, and the second thermometer is mounted on the annular mounting bracket. After the operator fixes the centrifuge tube to the fixing mechanism, the two sets of second hydraulic cylinders push the annular mounting bracket down, immersing the fixing mechanism in the hot water inside the cavity of the cylinder. The second thermometer detects the temperature of the centrifuge tube, facilitating the control of the two sets of second hydraulic cylinders to adjust the height.

[0012] Preferably, the fixing mechanism includes a protective cage, multiple sets of positioning plates, a mounting base, and multiple sets of rubber rings. The protective cage is rotatably mounted on the annular mounting frame and slidably mounted on the positioning strip. Multiple sets of positioning plates are mounted on the protective cage and have positioning holes. The mounting base is mounted on the protective cage and has multiple sets of positioning holes. The positioning holes on the positioning plates and the mounting base correspond one-to-one. Multiple sets of rubber rings are installed in the multiple sets of positioning holes. The operator fixes the centrifuge tube in the positioning holes of the positioning plates and the mounting base. The rubber rings prevent the centrifuge tube from colliding or scratching with the positioning plates. The operator adds hot water into the cavity of the cylinder, and then the sealing cap descends to seal the cavity. The lifting mechanism moves the centrifuge tube up and down in the cavity of the cylinder to facilitate adjustment of the soaking depth. The positioning strip drives the fixing mechanism to rotate so that the centrifuge tube is heated evenly.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: when activating a large amount of enzyme solution, the enzyme solution is directly added into the activation box, the heating mechanism maintains the temperature required for enzyme solution activation, and the stirring mechanism stirs the enzyme solution to make the enzyme solution heat evenly. When activating the enzyme solution in the centrifuge tube, the centrifuge tube is directly placed on the fixing mechanism, the lifting mechanism adjusts the height of the centrifuge tube, and the stirring mechanism drives the fixing mechanism and the centrifuge tube to rotate so that they are heated evenly. Attached Figure Description

[0014] Figure 1 This is a front view cross-sectional structural diagram of this utility model;

[0015] Figure 2 This is a cross-sectional isometric structural diagram of the activation box and heating mechanism of this utility model;

[0016] Figure 3 This is a partially enlarged cross-sectional isometric structural schematic diagram of the stirring mechanism of this utility model;

[0017] Figure 4 This is a partially enlarged cross-sectional isometric structural diagram of the lifting mechanism and fixing mechanism of this utility model.

[0018] The attached diagram is labeled as follows: 01, Activation box; 11, Insulation box; 12, Cylinder; 13, First thermometer; 14, Support; 15, First hydraulic cylinder; 16, Sealing cover; 02, Heating mechanism; 21, Heater; 22, Circulation pump; 23, Water suction pipe; 24, Water delivery pipe; 03, Stirring mechanism; 31, Electric motor; 32, Reducer; 33, Rotating shaft; 34, Stirring blade; 35, Positioning strip; 04, Lifting mechanism; 41, Second hydraulic cylinder; 42, Annular mounting plate; 43, Second thermometer; 05, Fixing mechanism; 51, Protective cage; 52, Positioning plate; 53, Placement seat; 54, Rubber ring. Detailed Implementation

[0019] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete. Example 1

[0020] This utility model discloses an enzyme solution constant temperature activation device, comprising an activation chamber 01; it also includes a heating mechanism 02, a stirring mechanism 03, a lifting mechanism 04, and a fixing mechanism 05. The heating mechanism 02 is installed on the activation chamber 01 and heats the water; the stirring mechanism 03 is installed on the activation chamber 01 and stirs the enzyme solution; the lifting mechanism 04 is installed on the activation chamber 01 and drives the fixing mechanism 05 to rise and fall; the fixing mechanism 05 is installed on the lifting mechanism 04 and supports the centrifuge tubes; the activation chamber 01 includes an insulation box 11, a cylinder 12, a support 14, two sets of first hydraulic cylinders 15, and a sealing cover 16. The bottom end of the insulation box 11 is connected to the ground, and the cylinder 12 is installed inside the insulation box 11, forming a sandwich between the cylinder 12 and the insulation box 11. The interlayer is connected to the water supply pipe. The interior of the cylinder 12 has a cavity. The bottom end of the support 14 is connected to the top end of the insulation box 11. Both sets of first hydraulic cylinders 15 are mounted on the support 14. The top end of the sealing cover 16 is connected to the bottom ends of the two sets of first hydraulic cylinders 15. The heating mechanism 02 includes a heater 21, a circulating pump 22, a water suction pipe 23, and a water delivery pipe 24. The heater 21 is installed inside the interlayer. The circulating pump 22 is installed on the insulation box 11. The water suction pipe 23 is installed on the circulating pump 22 and communicates with the bottom end of the interlayer. The water delivery pipe 24 is installed on the circulating pump 22 and communicates with the top end of the interlayer. The stirring mechanism 03 includes a motor 31, a reducer 32, a rotating shaft 33, stirring blades 34, and positioning bars 35. The motor 31... The bottom end is connected to the top end of the sealing cover 16, the bottom end of the reducer 32 is connected to the top end of the sealing cover 16, the rotating shaft 33 is rotatably mounted on the sealing cover 16 and longitudinally connected to the reducer 32, the stirring fan blade 34 is mounted on the rotating shaft 33, and the positioning strip 35 is mounted on the rotating shaft 33; it also includes three sets of first thermometers 13 installed in the cavity of the cylinder 12, the three sets of first thermometers 13 being distributed at different heights in the cavity; when it is working, firstly, the water supply pipe delivers water to the jacket, the heater 21 heats the water in the jacket, the circulation pump 22 is started, the circulation pump 22 draws out the hot water at the bottom end of the jacket through the water extraction pipe 23, and delivers the hot water to the top end pipe of the jacket through the water delivery pipe 24, so that the water in the jacket is heated evenly and kept at the same temperature. The temperature is maintained by the insulated box 11. Then, the staff adds the enzyme solution into the cavity of the cylinder 12. Two sets of first hydraulic cylinders 15 push the sealing cover 16 down to seal the cavity, facilitating enzyme activation. The staff starts the motor 31, which drives the rotating shaft 33 to rotate through the reducer 32. The rotating shaft 33 drives the stirring blades 34 to rotate, stirring the enzyme solution to ensure uniform heating. Three sets of first thermometers 13 are set up to facilitate temperature detection of the enzyme solution. When the temperature difference between the upper and lower layers of the enzyme solution is detected to be too large, the water flow rate of the circulating pump 22 is increased through an electrical signal to ensure uniform heating at different heights of the cylinder 12. At the same time, the speed of the motor 31 is increased through an electrical signal to ensure uniform mixing of the enzyme solution. Example 2

[0021] like Figures 1 to 4 As shown, this utility model discloses an enzyme solution constant temperature activation device, based on Example 1. The lifting mechanism 04 includes two sets of second hydraulic cylinders 41, an annular mounting frame 42, and a second thermometer 43. Both sets of second hydraulic cylinders 41 are mounted on the sealing cover 16. The top end of the annular mounting frame 42 is connected to the bottom end of the two sets of second hydraulic cylinders 41. The second thermometer 43 is mounted on the annular mounting frame 42. The fixing mechanism 05 includes a protective cage 51, multiple sets of positioning plates 52, a mounting base 53, and multiple sets of rubber rings 54. The protective cage 51 is rotatably mounted on the annular mounting frame 42 and slidably mounted on the positioning strip 35. The multiple sets of positioning plates 52 are all mounted on the sealing cover 16. Positioning holes are opened on the protective cage 51 and the positioning plate 52. The mounting base 53 is installed on the protective cage 51 and has multiple sets of positioning holes. The positioning holes on the positioning plate 52 and the mounting base 53 correspond one-to-one. Multiple sets of rubber rings 54 are installed in the multiple sets of positioning holes respectively. When it is working, firstly, the water supply pipe delivers water to the interlayer. The heater 21 heats the water in the interlayer. The circulation pump 22 is started. The circulation pump 22 draws out the hot water at the bottom of the interlayer through the water extraction pipe 23 and delivers the hot water to the top pipe of the interlayer through the water delivery pipe 24, so that the water in the interlayer is heated evenly and kept at the same temperature. The heat preservation box 11 keeps the water warm. Then, the staff adds enzyme solution. The enzyme solution is inserted into the cavity of the cylinder 12. Two sets of first hydraulic cylinders 15 push the sealing cover 16 down to seal the cavity, facilitating enzyme activation. The operator starts the motor 31, which drives the rotating shaft 33 to rotate via the reducer 32. The rotating shaft 33 drives the stirring blades 34 to rotate, stirring the enzyme solution and ensuring uniform heating. Three sets of first thermometers 13 are installed to facilitate temperature monitoring of the enzyme solution. When a large temperature difference is detected between the upper and lower layers of the enzyme solution, the water flow rate of the circulating pump 22 is increased via an electrical signal to ensure uniform heating at different heights of the cylinder 12. At the same time, the speed of the motor 31 is increased via an electrical signal to ensure uniform heating of the enzyme solution. During the activation of the enzyme solution in the centrifuge tube, the operator fixes the centrifuge tube in the positioning holes of the positioning plate 52 and the mounting base 53. A rubber ring 54 is used to prevent the centrifuge tube from colliding and scratching with the positioning plate 52. The operator adds hot water into the cavity of the cylinder 12, and then the sealing cap 16 descends to seal the cavity. Two sets of second hydraulic cylinders 41 push the annular mounting bracket 42 down, so that the fixing mechanism 05 is immersed in the hot water in the cavity of the cylinder 12. The second thermometer 43 detects the temperature of the centrifuge tube, which facilitates the control of the two sets of second hydraulic cylinders 41 to adjust the height. The positioning bar 35 drives the fixing mechanism 05 to rotate so that the centrifuge tube is heated evenly.

[0022] The electric motor 31 and the reducer 32 of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0023] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. An enzyme solution isothermal activation device, comprising an activation chamber (01); characterized in that, It also includes a heating mechanism (02), a stirring mechanism (03), a lifting mechanism (04), and a fixing mechanism (05). The heating mechanism (02) is installed on the activation box (01) and heats the water. The stirring mechanism (03) is installed on the activation box (01) and stirs the enzyme solution. The lifting mechanism (04) is installed on the activation box (01) and drives the fixing mechanism (05) to rise and fall. The fixing mechanism (05) is installed on the lifting mechanism (04) and supports the centrifuge tube.

2. The enzyme solution isothermal activation device as described in claim 1, characterized in that, The activation box (01) includes an insulated box (11), a cylinder (12), a support (14), two sets of first hydraulic cylinders (15) and a sealing cover (16). The bottom end of the insulated box (11) is connected to the ground. The cylinder (12) is installed inside the insulated box (11). A sandwich is formed between the cylinder (12) and the insulated box (11). The sandwich is connected to the water supply pipe. The inside of the cylinder (12) is provided with a cavity. The bottom end of the support (14) is connected to the top end of the insulated box (11). Both sets of first hydraulic cylinders (15) are installed on the support (14). The top end of the sealing cover (16) is connected to the bottom end of the two sets of first hydraulic cylinders (15).

3. The enzyme solution isothermal activation device as described in claim 2, characterized in that, The heating mechanism (02) includes a heater (21), a circulation pump (22), a water pumping pipe (23), and a water supply pipe (24). The heater (21) is installed inside the interlayer, the circulation pump (22) is installed on the insulation box (11), the water pumping pipe (23) is installed on the circulation pump (22) and communicates with the bottom of the interlayer, and the water supply pipe (24) is installed on the circulation pump (22) and communicates with the top of the interlayer.

4. The enzyme solution isothermal activation device as described in claim 2, characterized in that, The stirring mechanism (03) includes a motor (31), a reducer (32), a rotating shaft (33), stirring blades (34), and a positioning bar (35). The bottom end of the motor (31) is connected to the top end of the sealing cover (16), the bottom end of the reducer (32) is connected to the top end of the sealing cover (16), the rotating shaft (33) is rotatably mounted on the sealing cover (16) and longitudinally connected to the reducer (32), the stirring blades (34) are mounted on the rotating shaft (33), and the positioning bar (35) is mounted on the rotating shaft (33).

5. The enzyme solution isothermal activation device as described in claim 2, characterized in that, It also includes three sets of first thermometers (13) installed inside the cavity of the cylinder (12), with the three sets of first thermometers (13) distributed at different heights inside the cavity.

6. The enzyme solution isothermal activation device as described in claim 2, characterized in that, The lifting mechanism (04) includes two sets of second hydraulic cylinders (41), an annular mounting bracket (42), and a second thermometer (43). Both sets of second hydraulic cylinders (41) are mounted on the sealing cover (16). The top of the annular mounting bracket (42) is connected to the bottom of the two sets of second hydraulic cylinders (41). The second thermometer (43) is mounted on the annular mounting bracket (42).

7. The enzyme solution isothermal activation device as described in claim 6, characterized in that, The fixing mechanism (05) includes a protective cage (51), multiple positioning plates (52), a mounting base (53), and multiple rubber rings (54). The protective cage (51) is rotatably mounted on the annular mounting bracket (42) and slidably mounted on the positioning strip (35). Multiple positioning plates (52) are all mounted on the protective cage (51) and have positioning holes. The mounting base (53) is mounted on the protective cage (51) and has multiple positioning holes. The positioning holes on the positioning plates (52) and the mounting base (53) correspond one-to-one. Multiple rubber rings (54) are respectively installed in multiple positioning holes.

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