High-throughput cell screening plate

Abstract

The utility model relates to a high-throughput cell screening plate, which belongs to a biological medicine research and development device and is used for qualitatively or quantitatively screening cells for expressing or secreting specific proteins or other biochemical molecules from a group of cells. The device comprises a bottom plate, a mesh and a cover plate, a large groove with a flat bottom is formed in the center of the bottom plate, small grooves are formed in the edge of the bottom plate, and the mesh is of a latticed structure and can be embedded in the large groove and divide the space in the groove. The device has the beneficial effects that the screening flux and the consumed time are superior to those of a limited dilution method, the manufacturing cost and the operation difficulty are superior to those of a micro-engraving method, and the device is suitable for screening conditions that markers are expressed on the surfaces of cells and secreted in the environment.

Description

Technical Field

[0001] This utility model relates to a biomedical research and development device, and more specifically, to a high-throughput cell screening plate. Background Technology

[0002] Biological macromolecules are a class of biological macromolecules produced using modern biotechnology for the diagnosis, treatment, and prevention of diseases. These include monoclonal antibodies, recombinant proteins, peptides, enzymes, and cytokines. These drugs are characterized by strong targeting, high specificity, low toxicity, and high sensitivity, demonstrating significant efficacy in the treatment of various major diseases, including malignant tumors, immune system diseases, metabolic diseases, genetic diseases, and infectious diseases.

[0003] Cell screening refers to the process of selecting cells from a population that meet specific functional requirements, followed by monoclonalization and amplification. It is a crucial technique used in antibody screening (such as hybridoma screening and single B-cell screening) and the screening of engineered cell lines for antibody production. Existing cell screening methods include limiting dilution, flow cytometry, and microengraving. Each method has its limitations: limiting dilution is labor-intensive and time-consuming; flow cytometry is only suitable when cell screening markers are expressed on the cell surface; and microengraving requires photolithography equipment and demands highly skilled operators. Summary of the Invention

[0004] This invention provides a high-throughput cell screening plate for qualitatively or quantitatively screening cells that express or secrete specific proteins or other biochemical molecules from a group of cells.

[0005] The technical solution of this utility model is:

[0006] A high-throughput cell screening plate, such as Figure 1 , Figure 2 As shown, it includes a base plate 1, a mesh grid 2, and a cover plate 3.

[0007] The base plate 1 is rectangular when viewed from above, with a large, flat-bottomed groove 4 in the center and smaller grooves 5 along the edges. The large groove 4 is used to lay a semi-solid culture medium containing cells, and the smaller grooves 5 are used to hold water or an aqueous solution to regulate the humidity of the microenvironment. Optionally, the base plate 1 has a level 6 to detect its levelness; and adjustment knobs 7 at the four corners of the base plate 1 can be used to adjust its level.

[0008] The mesh 2 is a grid-like structure that can be fitted into the large groove 4 and divide the space inside the groove into several small blocks.

[0009] The cover plate 3 is fastened to the base plate 1, serving a sealing and protective function. Optionally, the cover plate 3 has antibacterial and ventilated holes 8.

[0010] The base plate 1 is made of transparent rigid plastic, such as polystyrene, polypropylene, polycarbonate or polymethyl methacrylate, and the mesh 2 is made of transparent rigid plastic or a stable and corrosion-resistant metal, such as stainless steel or titanium alloy.

[0011] The method of using this utility model is as follows:

[0012] Step 1: Place the base plate 1 on the experimental platform, and use the knob 7 to adjust the level 6 to ensure that the base plate 1 is level.

[0013] Step 2: Pour the semi-solid culture medium containing cells into the large groove 4 and spread it evenly on the bottom. Place the grid 2 into the large groove 4, pour water into the small groove 5, cover it with the cover plate 3, and place it in a cell culture incubator for a period of time.

[0014] Step 3: Add fluorescently labeled antibodies or antigens (antibodies or antigens that have no effect on cell activity and are stable can also be added to the cell culture medium before culturing) to the large groove 4, place it in the detector, and qualitatively and quantitatively detect the labeled positive cells.

[0015] Step 4: Positive cell colonies are removed from the semi-solid culture medium by micromanipulation, then cloned, amplified, and further identified to obtain cell lines that meet the required traits.

[0016] The advantages of this invention are that it has a higher screening throughput and shorter screening time than the limiting dilution method, lower manufacturing cost and easier operation than the micro-engraving method, and it is applicable to both screening markers expressed on the cell surface and secreted in the environment. Attached Figure Description

[0017] Figure 1 : A longitudinal cross-sectional view of a high-throughput cell screening plate before assembly.

[0018] Figure 2 Top view of a high-throughput cell screening plate after the cover is opened.

[0019] Attached reference numerals: 1-base plate, 2-mesh grid, 3-cover plate, 4-large groove, 5-small groove, 6-level, 7-adjustment knob, 8-antibacterial and breathable hole. Detailed Implementation

[0020] Example 1: Structure of a high-throughput cell screening plate

[0021] A high-throughput cell screening plate, such as Figure 1 , Figure 2 As shown, it includes a base plate 1, a mesh grid 2, and a cover plate 3.

[0022] The base plate 1 is rectangular when viewed from above, with a large, flat-bottomed groove 4 in the center and smaller grooves 5 along the edges. The large groove 4 is used to lay a semi-solid culture medium containing cells, and the smaller grooves 5 are used to hold water or an aqueous solution to regulate the humidity of the microenvironment. The base plate 1 has a level 6 to detect its levelness; there are adjustment knobs 7 at the four corners of the base plate 1 to adjust its level.

[0023] The aforementioned mesh 2 is a grid-like structure that can be fitted into the large groove 4 and divide the space within the groove into several small blocks.

[0024] The cover plate 3 is fastened to the base plate 1, serving a sealing and protective function. The cover plate 3 has antibacterial and ventilated holes 8.

[0025] The base plate 1 is made of transparent rigid plastic polystyrene, and the mesh 2 is made of stainless steel.

[0026] Example 2: A method for using a high-throughput cell screening plate

[0027] The method of using this utility model is as follows:

[0028] Step 1: Place the base plate 1 on the experimental platform, and use the knob 7 to adjust the level 6 to ensure that the base plate 1 is level.

[0029] Step 2: Pour the semi-solid culture medium containing cells into the large groove 4 and spread it evenly on the bottom. Place the grid 2 into the large groove 4, pour water into the small groove 5, cover it with the cover plate 3, and place it in a cell culture incubator for a period of time.

[0030] Step 3: Add fluorescently labeled antibodies or antigens (antibodies or antigens that have no effect on cell activity and are stable can also be added to the cell culture medium before culturing) to the large groove 4, place it in the detector, and qualitatively and quantitatively detect the labeled positive cells.

[0031] Step 4: Positive cell colonies are removed from the semi-solid culture medium by micromanipulation, then cloned, amplified, and further identified to obtain cell lines that meet the required traits.

Claims

1. A high-throughput cell screening plate, characterized in that, Includes a base plate (1), a mesh grid (2), and a cover plate (3). The base plate (1) is rectangular when viewed from above, with a large, flat-bottomed groove (4) in the center and small grooves (5) on the edges. The mesh grid (2) is a grid structure that can be fitted into the large groove (4) and divide the space inside the groove. The cover plate (3) can be fastened to the base plate (1) to provide sealing and protection.

2. The high-throughput cell screening plate according to claim 1, characterized in that, The base plate (1) has a level (6) for detecting the level of the base plate (1), and there are adjustment knobs (7) at the four corners of the base plate (1) for adjusting the level of the base plate (1).

3. The high-throughput cell screening plate according to claim 1, characterized in that, The cover plate (3) has antibacterial and breathable holes (8).

4. The high-throughput cell screening plate according to claim 1, characterized in that, The base plate (1) is made of polystyrene, polypropylene, polycarbonate or polymethyl methacrylate.

5. The high-throughput cell screening plate according to claim 1, characterized in that, The mesh (2) is made of polystyrene, polypropylene, polycarbonate, polymethyl methacrylate, stainless steel or titanium alloy.

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