Constant-temperature oscillation liquid culture device for spores

Abstract

The utility model provides a spore constant-temperature oscillation liquid culture device. The spore constant-temperature oscillation liquid culture device comprises a box body and a placement frame, and the placement frame is arranged in an inner cavity of the box body; the plurality of placing holes penetrate through the placing rack and are used for placing test bottles; the positioning conical seat is fixedly mounted on the bottom wall of the placement frame and is aligned with the placement hole, and a plurality of clamping pieces inclined towards one side of the axis of the placement hole are arranged on the side wall of the positioning conical seat; the temperature control assembly is installed in the inner cavity of the box body and used for controlling the temperature of a water source in the inner cavity of the box body; the driving assembly is installed in the inner cavity of the box body, the output end of the driving assembly is in power connection with the placing frame to drive the placing frame to vibrate, the clamping pieces provide reverse clamping force for the test bottles, the risk of relative movement of the test bottles and the placing frame is reduced, and at the moment, the liquid level in the test bottles can be reduced to be below the water bath liquid level; the culture effect of microorganisms is improved, and the risks of up-and-down floating and overturning of the test bottles are reduced.

Description

Technical Field

[0001] This application relates to the field of biological culture equipment, and more particularly to a spore isothermal oscillating liquid culture device. Background Technology

[0002] A shaking incubator is a laboratory biological sample culture device. In order to improve the temperature stability during biological culture, a water bath shaker is usually used, in which test bottles containing microorganisms are placed in water.

[0003] During the placement of the test bottles, in order to avoid the test bottles floating or tipping over, the liquid level in the test bottles is slightly higher than the water bath level. The microorganisms above the water bath level are in a different temperature environment than the microorganisms at the bottom, which affects the microbial culture effect.

[0004] After the test bottle is placed inside the water bath, it is not fixed to the rack. As the water level fluctuates in the incubator, the relative movement between the test bottle and the rack will increase, which can easily cause microorganisms to burst out of the test bottle and contaminate the water in the incubator.

[0005] Therefore, how to design a shaking liquid culture device that reduces the relative movement between the test bottle and the rack has become a technical problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0006] This application provides a spore isothermal oscillating liquid culture device to at least solve the above-mentioned technical problems existing in the prior art.

[0007] A spore isothermal oscillating liquid culture device is provided, including a box and a placement rack, the placement rack being disposed in the inner cavity of the box;

[0008] Placement holes, several of which pass through the placement rack for placing test bottles;

[0009] The positioning cone is fixedly installed on the bottom wall of the placement frame and aligned with the placement hole. The side wall of the positioning cone is provided with several clamping pieces that are inclined towards the axis of the placement hole.

[0010] Temperature control component, installed inside the chamber to control the temperature of the water source inside the chamber;

[0011] The drive assembly is installed inside the housing. The output end of the drive assembly is connected to the placement rack for driving the placement rack to vibrate.

[0012] In one embodiment, the temperature control assembly includes a central control unit and a control panel, a heating wire, and a temperature sensor electrically connected to the central control unit. The temperature sensor is installed in the inner cavity of the chamber, and the detection end of the temperature sensor extends below the water source in the inner cavity of the chamber. The heating wire is disposed in the water source in the inner cavity of the chamber.

[0013] In one embodiment, the drive assembly includes a drive motor, a transmission assembly, and an elastic element. The side wall of the placement rack is slidably connected to the inner wall of the housing. The output end of the drive motor is poweredly connected to the input end of the transmission assembly. The output end of the transmission assembly is connected to the placement rack through the elastic element.

[0014] In one embodiment, the transmission assembly includes a push plate and a tension spring. The push plate is placed horizontally in the inner cavity of the housing and is located below the placement frame. One end of the tension spring is fixedly connected to the bottom wall of the push plate, and the other end of the tension spring is fixedly connected to the inner wall of the housing.

[0015] In one embodiment, the transmission assembly further includes a support plate, which is fixedly installed on the side wall of the housing cavity. The support plate is located below the push plate, and one end of the tension spring is fixedly connected to the bottom wall of the push plate, while the other end of the tension spring is fixedly connected to the top wall of the support plate.

[0016] In one embodiment, four sets of support plates are provided and located below the four corners of the push plate.

[0017] In one embodiment, the support plate is provided with a first guide hole, and the bottom wall of the push plate is provided with a first guide rod that is inserted into the first guide hole, and a tension spring is sleeved on the outside of the first guide rod.

[0018] In one embodiment, the transmission assembly further includes a transmission rod and a cam. The transmission rod is fixedly connected to the output shaft of the drive motor, and the cam is fixedly mounted on the transmission rod. The peripheral wall of the cam abuts against the bottom wall of the push plate.

[0019] In one embodiment, the elastic element includes a spring, one end of which is fixedly connected to the bottom wall of the placement rack, and the other end of which is fixedly connected to the top wall of the push plate.

[0020] In one embodiment, the push plate is provided with a second guide hole, and the bottom wall of the placement frame is fixedly provided with a second guide rod passing through the second guide hole, and a spring is sleeved on the outside of the second guide rod.

[0021] Compared with the prior art, the spore isothermal oscillating liquid culture device of this application has the following beneficial effects:

[0022] In this application, the test bottle is installed on the rack through the placement hole. At this time, the test bottle passes through the positioning cone seat, and the outer wall of the test bottle pushes the clamping plate away from the axis of the placement hole. The clamping plate provides a reverse clamping force to the test bottle, thereby fixing the test bottle to the rack and reducing the risk of relative movement between the test bottle and the rack. At this time, the liquid level in the test bottle can be lowered to below the water bath liquid surface, so that the microorganisms in the test bottle can be heated evenly, improving the microbial culture effect, while reducing the risk of the test bottle floating up and down and tipping over. The rack transmits the power of the drive component to the test bottle, realizing high-frequency vibration of the test bottle, preventing microorganisms from accumulating at the bottom of the test bottle, and increasing the dissolved oxygen inside the test bottle, thereby improving the survival rate of microorganisms.

[0023] It should be understood that the description in this section is not intended to identify key or essential features of the embodiments of this application, nor is it intended to limit the scope of this application. Other features of this application will become readily apparent from the following description. Attached Figure Description

[0024] The above and other objects, features, and advantages of exemplary embodiments of this application will become readily apparent from the following detailed description taken in conjunction with the accompanying drawings. Several embodiments of this application are illustrated in the drawings by way of example and not limitation, in which:

[0025] In the accompanying drawings, the same or corresponding reference numerals indicate the same or corresponding parts.

[0026] Figure 1 A schematic diagram of the first angle of this application is shown;

[0027] Figure 2 A second-angle schematic diagram of this application is shown;

[0028] Figure 3 A cross-sectional view of this application is shown;

[0029] Figure 4 A schematic diagram of the internal structure of this application is shown;

[0030] Figure 5 A partial unfolded schematic diagram of this application is shown;

[0031] Figure 6 A schematic diagram of the positioning cone seat of this application is shown.

[0032] Explanation of the labels in the diagram:

[0033] 1. Container; 11. Drain pipe; 12. Water inlet pipe;

[0034] 2. Placement rack; 21. Placement hole; 22. Second guide rod;

[0035] 3. Positioning cone seat; 31. Clamping plate;

[0036] 4. Temperature control components; 41. Central control unit; 42. Control panel; 43. Heating wire; 44. Temperature sensor;

[0037] 5. Drive assembly; 51. Drive motor; 52. Transmission assembly; 521. Push plate; 5211. First guide rod; 5212. Second guide hole; 522. Tension spring; 523. Support plate; 5231. First guide hole; 524. Transmission rod; 525. Cam; 53. Elastic element; 531. Spring. Detailed Implementation

[0038] To make the objectives, features, and advantages of this application more apparent and understandable, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0039] like Figure 1 As shown, the device includes a box 1, with the top opening of the box 1 facing upwards to place test bottles into the inner cavity of the box 1, thus completing the placement of the test bottles containing microorganisms. The bottom of the box 1 is hinged with a lid. When it is necessary to perform constant temperature vibration culture of the microorganisms in the test bottles, the lid closes the top opening of the box 1, reducing the heat exchange between the external temperature and the internal temperature of the box 1, and reducing the wear and tear on the equipment.

[0040] To improve the stability of the test bottle heating temperature, in this embodiment, as follows: Figure 3 As shown, a water source is placed inside the chamber 1 to maintain the water source within a constant and stable range. Test bottles containing microorganisms are placed inside the water source, which means that a water bath heating method is used to maintain a constant temperature environment for microbial culture and improve the stability of the microbial culture environment.

[0041] In order to control the temperature of the water source inside tank 1, such as Figure 1 , Figure 2 and Figure 3 As shown, it includes a temperature control component 4, which is used to control the water source temperature and maintain the water source temperature within a small range of fluctuation, with the temperature fluctuating within the range of 0.5℃.

[0042] Specifically, the temperature control component 4 includes a central control unit 41, a control panel 42, a heating wire 43, and a temperature sensor 44. The control panel 42, the heating wire 43, and the temperature sensor 44 are all electrically connected to the central control unit 41. The detection end of the temperature sensor 44 and the heating wire 43 are immersed in the water source inside the chamber 1. The control panel 42 and the central control unit 41 are installed on the outer wall of the chamber 1. The temperature sensor 44 feeds back the temperature of the water source to the central control unit 41. After the central control unit 41 judges the temperature, it sends an electrical signal, thereby heating the water source through the heating wire 43. The temperature of the water source can be set through the control panel 42.

[0043] It is worth noting that the temperature control component 4 is an existing product on the market, and no improvements have been made to the temperature control component 4. Therefore, we will not elaborate on the temperature control component 4 here.

[0044] Under normal circumstances, a placement rack 2 is placed above the water source inside the chamber 1. The placement rack 2 has several vertically penetrating placement holes 21, which are used to insert test tubes or test bottles to store microbial culture medium, thereby providing a suitable environment for the cultivation of microorganisms.

[0045] When test tubes or bottles are placed inside placement hole 21, the diameter of placement hole 21 cannot be completely matched with the outer diameter of test tubes or bottles, which may cause the test tubes or bottles to move vertically relative to the placement rack 2. In this case, the liquid level of the microbial culture medium placed inside the test tubes or bottles should be higher than the liquid level of the water source to reduce the risk of the test tubes or bottles floating or tipping over.

[0046] However, in this case, the temperature of the microbial culture medium above the water source differs from the temperature of the microbial culture medium at the bottom of the water source, which will affect the effect of microbial culture.

[0047] Therefore, in this embodiment, as Figure 3 and Figure 6 As shown, the bottom wall of the placement rack 2 is fixedly provided with a positioning cone 3 aligned with the placement hole 21. Here, the positioning cone 3 is funnel-shaped, with a large opening at the upper end and a small opening at the lower end. The side wall of the positioning cone 3 is provided with several clamping pieces 31, wherein the bottom end of the clamping piece 31 is inclined towards the axis of the placement hole 21. When the test bottle or test tube is inserted into the positioning cone 3, the clamping piece 31 applies a clamping force to the outer wall of the test tube or test bottle, thereby fixing the test tube or test bottle on the placement rack 2, preventing the test tube or test bottle from moving relative to the placement rack 2, reducing the impact of water source fluctuations on the test tube or test bottle, and reducing the risk of the test tube or test bottle tipping over.

[0048] It is worth noting here that, as Figure 6As shown, the clamping piece 31 is formed by opening a slot on the side wall of the positioning cone seat 3.

[0049] In order to achieve the agitation of the culture medium inside the test bottle or test tube, in this embodiment, as follows: Figure 3 and Figure 4 As shown, the device includes a drive component 5. Specifically, the drive component 5 includes a drive motor 51 and a transmission component 52. One end of the drive motor 51 is connected to the input end of the transmission component 52, and the output end of the transmission component 52 is connected to the placement rack 2. The transmission component 52 drives the placement rack 2 to vibrate at high frequency, thereby driving the oscillation of the culture medium inside the test tube or bottle and reducing the risk of microorganisms accumulating at the bottom of the culture medium.

[0050] Specifically, such as Figure 3 and Figure 5 As shown, the transmission assembly 52 includes a push plate 521, a tension spring 522, a transmission rod 524, a cam 525, and an elastic element 53. The push plate 521 is horizontally arranged in the inner cavity of the housing 1, and is positioned below the water source liquid level and below the placement frame 2. One end of the tension spring 522 is fixedly installed on the bottom wall of the push plate 521, and the other end of the tension spring 522 is fixedly installed on the side wall of the inner cavity of the housing 1. The transmission rod 524 is fixedly connected to the output shaft of the drive motor 51. The cam 525 is fixedly installed on the transmission rod 524, and the peripheral wall of the cam 525 abuts against the bottom wall of the push plate 521. The placement frame 2 is connected to the push plate 521 through the elastic element 53.

[0051] With the above settings, the drive motor 51 drives the transmission rod 524 to rotate, which in turn drives the cam 525 to rotate. When the point of the cam 525 that is furthest from its own axis comes into contact with the push plate 521, the tension spring 522 is stretched to its longest state. As the cam 525 continues to rotate, the tension spring 522 drives the push plate 521 to move down. As the cam 525 continues to rotate, the push plate 521 moves up and down reciprocally. Through the elastic element 53, the placement rack 2 can move up and down reciprocally. Since the placement rack 2 is fixedly connected to the test tube or test bottle through the positioning cone seat 3, high-frequency oscillation of the culture medium inside the test tube or test bottle can be realized.

[0052] It is worth noting that, since the placement frame 2 and the push plate 521 are elastically connected through the elastic element 53, the vibration frequency of the placement frame 2 can be increased.

[0053] Specifically, the elastic element 53 includes a spring 531, one end of which is fixedly connected to the bottom wall of the placement frame 2, and the other end of which is fixedly connected to the top wall of the push plate 521.

[0054] like Figure 3 and Figure 5As shown, it also includes a support plate 523, wherein the support plate 523 is provided with four sets respectively placed below the four corners of the push plate 521. The support plate 523 is fixedly connected to the side wall of the inner cavity of the box 1. Four tension springs 522 are provided. One end of the tension spring 522 is fixedly connected to the bottom wall of the push plate 521, and the other end of the tension spring 522 is fixedly connected to the top wall of the support plate 523.

[0055] To achieve stable lifting and lowering of the push plate 521, in this embodiment, as follows: Figure 3 , Figure 4 and Figure 5 As shown, the support plate 523 is provided with a first guide hole 5231, and the bottom wall of the push plate 521 is fixedly provided with a first guide rod 5211 passing through the first guide hole 5231. The first guide rod 5211 is arranged along the vertical direction of the box 1. The outer wall of the first guide rod 5211 is slidably connected to the inner wall of the first guide hole 5231. The tension spring 522 is sleeved on the outer side of the first guide rod 5211.

[0056] Since the connection between the placement rack 2 and the push plate 521 is achieved using spring 531, there is a risk that the placement rack 2 may shift laterally after a test bottle is placed on it. Therefore, in this embodiment, as... Figure 5 As shown, the push plate 521 is provided with a second guide hole 5212, and the bottom wall of the placement frame 2 is fixedly provided with a second guide rod 22 passing through the second guide hole 5212. The spring 531 is sleeved on the outside of the second guide rod 22, thereby improving the stability of the placement frame 2 during vibration.

[0057] Furthermore, such as Figure 4 As shown, the bottom wall of the box 1 is also provided with a drain pipe 11 that communicates with the inner cavity of the box 1; the side wall of the box 1 is provided with a water inlet pipe 12 that communicates with the inner cavity of the box 1. Water can be injected into the inner cavity of the box 1 through the water inlet pipe 12, and the water inside the box 1 can be discharged out through the drain pipe 11, so as to realize the replacement of the water source.

[0058] It should be understood that the various forms of processes shown above can be used to rearrange, add, or delete steps. For example, the steps described in this disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution disclosed in this application can be achieved, and this is not limited herein.

[0059] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "a plurality of" means two or more, unless otherwise explicitly specified.

[0060] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A spore isothermal oscillating liquid culture device, comprising a housing (1), characterized in that, Placement rack (2), which is set in the inner cavity of the box (1); Placement holes (21) are provided, and several placement holes (21) pass through the placement rack (2) for placing test bottles; Positioning cone (3) is fixedly installed on the bottom wall of the placement frame (2) and aligned with the placement hole (21). The side wall of the positioning cone (3) is provided with several clamping pieces (31) that are inclined to one side of the axis of the placement hole (21). Temperature control component (4) is installed in the inner cavity of the box (1) to control the temperature of the water source in the inner cavity of the box (1); The drive assembly (5) is installed in the inner cavity of the housing (1). The output end of the drive assembly (5) is connected to the placement rack (2) to drive the placement rack (2) to vibrate.

2. The spore isothermal oscillating liquid culture device according to claim 1, characterized in that, The temperature control assembly (4) includes a central control unit (41) and a control panel (42), a heating wire (43), and a temperature sensor (44) electrically connected to the central control unit (41). The temperature sensor (44) is installed in the inner cavity of the housing (1), and the detection end of the temperature sensor (44) extends below the water source in the inner cavity of the housing (1). The heating wire (43) is located in the water source in the inner cavity of the housing (1).

3. The spore isothermal oscillating liquid culture device according to claim 1, characterized in that, The drive assembly (5) includes a drive motor (51), a transmission assembly (52), and an elastic element (53). The side wall of the placement rack (2) is slidably connected to the inner wall of the box (1). The output end of the drive motor (51) is poweredly connected to the input end of the transmission assembly (52). The output end of the transmission assembly (52) is connected to the placement rack (2) through the elastic element (53).

4. The spore isothermal oscillating liquid culture device according to claim 3, characterized in that, The transmission assembly (52) includes a push plate (521) and a tension spring (522). The push plate (521) is placed horizontally in the inner cavity of the housing (1) and is located below the placement frame (2). One end of the tension spring (522) is fixedly connected to the bottom wall of the push plate (521), and the other end of the tension spring (522) is fixedly connected to the inner wall of the housing (1).

5. The spore isothermal oscillating liquid culture device according to claim 4, characterized in that, The transmission assembly (52) also includes a support plate (523), which is fixedly installed on the side wall of the cavity of the housing (1). The support plate (523) is located below the push plate (521). One end of the tension spring (522) is fixedly connected to the bottom wall of the push plate (521), and the other end of the tension spring (522) is fixedly connected to the top wall of the support plate (523).

6. The spore isothermal oscillating liquid culture device according to claim 5, characterized in that, The support plate (523) is provided in four sets and is located below the four corners of the push plate (521).

7. The spore isothermal oscillating liquid culture device according to claim 5, characterized in that, The support plate (523) is provided with a first guide hole (5231), and the bottom wall of the push plate (521) is provided with a first guide rod (5211) that is inserted into the first guide hole (5231), and a tension spring (522) is sleeved on the outside of the first guide rod (5211).

8. A spore isothermal oscillating liquid culture device according to claim 5 or 7, characterized in that, The transmission assembly (52) also includes a transmission rod (524) and a cam (525). The transmission rod (524) is fixedly connected to the output shaft of the drive motor (51), and the cam (525) is fixedly mounted on the transmission rod (524). The peripheral wall of the cam (525) abuts against the bottom wall of the push plate (521).

9. The spore isothermal oscillating liquid culture device according to claim 8, characterized in that, The elastic element (53) includes a spring (531), one end of which is fixedly connected to the bottom wall of the placement frame (2), and the other end of which is fixedly connected to the top wall of the push plate (521).

10. The spore isothermal oscillating liquid culture device according to claim 9, characterized in that, The push plate (521) is provided with a second guide hole (5212), and the bottom wall of the placement frame (2) is fixed with a second guide rod (22) that passes through the second guide hole (5212). A spring (531) is sleeved on the outside of the second guide rod (22).

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