Volume-adjustable rapid lymphocyte separation tube

Abstract

The utility model belongs to the technical field of medical instruments, and particularly relates to a rapid lymphocyte separating tube with adjustable volume, which comprises a tube body, a separating device, a threaded section and a rotary moving rod, the threaded section is arranged on the inner wall of the middle of the tube body, the separating device is connected inside the tube body in a sliding manner through the threaded section, and the rotary moving rod is arranged on the inner wall of the middle of the tube body. The separating device is provided with a central separating hole for a sample to pass through during centrifugation, and the rotary moving rod is clamped with the separating device through the central separating hole. According to the device, the positions of the partition plates can be adjusted according to blood of different volumes, so that the requirement of rapidly separating lymphocytes from the blood of different volumes is met, and time and resources are saved.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a rapid lymphocyte separation tube with adjustable volume. Background Technology

[0002] Mononuclear cell extraction methods often involve adding Ficoll-hypaque (FH) or Percoll, followed by density gradient centrifugation to separate the cell components into distinct layers, thus achieving the separation of mononuclear cells. Currently, commercially available rapid centrifuge tubes are generally designed for 50ml cells because Ficoll-hypaque and blood are typically added in a 1:1 ratio, limiting the volume of blood separated per centrifuge tube to approximately 10-20ml. Separating a larger volume of blood may require more rapid centrifuge tubes, wasting both time and tubes.

[0003] Therefore, to address the aforementioned problems, a rapid lymphocyte separation tube with adjustable volume is proposed. Utility Model Content

[0004] This invention addresses the problems mentioned above by designing a volume-adjustable rapid lymphocyte separation tube. This device can adjust the position of the separator for different volumes of blood to meet the needs of rapid lymphocyte separation for different volumes of blood, saving time and resources.

[0005] To achieve the above objectives, this utility model provides a volume-adjustable rapid lymphocyte separation tube, comprising: a tube body, a separating device, a threaded section, and a rotating rod. The threaded section is provided on the inner wall of the middle part of the tube body. The separating device is slidably connected to the inside of the tube body through the threaded section. The separating device has a central separation hole for passing the sample through during centrifugation. The rotating rod is engaged with the separating device through the central separation hole.

[0006] In the above manner, the separator is engaged by a rotating rod, which can drive the separator to rotate. Through the drive of the threaded section, the vertical position can be adjusted inside the tube to accommodate different volumes of blood.

[0007] Furthermore, the separating device also includes a top plate and an annular barrel section. The top plate has a concave structure, and the annular barrel section is located at the lower part of the top plate. The outer wall of the annular barrel section is provided with a thread that mates with the threaded section. The central separating hole is located in the middle of the top plate.

[0008] Furthermore, the separating device also includes slots, which are semi-circular, and there are several slots arranged around the edge of the top plate.

[0009] In this way, both the central separation hole and the slot can be used to exchange the separation liquid and the sample on the separator plate during centrifugation.

[0010] Furthermore, the rotating rod includes a first long handle and a first handle. One end of the first long handle is fixedly connected to a snap-fit ​​part that matches the shape of the central separation hole, and the other end is fixedly connected to the first handle.

[0011] In the above manner, the snap-fit ​​part snaps into the separator through the central separation hole, causing the separator to move up and down.

[0012] Furthermore, it also includes a fixing device, which is installed on the lower part of the top plate and its end passes through the annular barrel and abuts against the inner wall of the tube. The rotating rod also includes a second long handle and a second handle. The middle part of the first long handle has a through hole, and the end of the second long handle is fixedly connected to the second handle. The second long handle passes through the through hole and is engaged with the fixing device.

[0013] Furthermore, the fixing device includes: an elliptical part, a rotating shaft, and an abutting device. A box body is fixedly connected to the lower part of the top plate. The box body is located below the central separation hole and has a hollow structure. The bottom of the box body is rotatably connected to the elliptical part through the rotating shaft. The abutting device is movably connected to both sides of the box body, with one end passing through the side wall of the box body and abutting against the elliptical part, and the other end passing through the annular barrel part and abutting against the inner wall of the tube body.

[0014] Furthermore, the abutting device includes: a sliding rod, an arc-shaped connector, a sleeve, a limiting ring, an elastic element, and a support rod. One end of the sliding rod is fixedly connected to the arc-shaped connector, which abuts against the elliptical portion. The sleeve is sleeved on the outside of the sliding rod, and the sleeve is fixedly connected to the lower part of the top plate through the support rod. Limiting rings are fixedly connected to both the inner wall of the sleeve and the outer wall of the sliding rod. The elastic element is sleeved on the outside of the sliding rod, and the elastic element is located between the limiting rings.

[0015] After the position of the separating device is adjusted by inserting the rotating rod into the central separation hole in the above manner, the separating device needs to be fixed. The second long handle is inserted through the through hole and inserted into the notch at the top of the elliptical part. The elliptical part is rotated, and the long end of the elliptical part abuts against the arc-shaped connector, driving the slide rod to move forward. The other end of the slide rod passes through the annular barrel and abuts against the inner wall of the tube, which achieves the effect of fixing the separating device. When it is necessary to remove it, the elliptical part is rotated again, and the elliptical part cancels the abutment of the slide rod. Under the action of the elastic element, the slide rod retracts, canceling the abutment effect on the tube.

[0016] In summary, this utility model has the following advantages and beneficial technical effects:

[0017] 1. This utility model provides a volume-adjustable rapid lymphocyte separation tube that can adjust the position of the separator plate for different volumes of blood to meet the needs of rapid lymphocyte separation for different volumes of blood;

[0018] 2. The separation device of the adjustable-volume rapid lymphocyte separation tube of this utility model can be fixed by a reinforcement device after the position is adjusted to prevent the separation device from shaking during centrifugation.

[0019] 3. The adjustable-volume rapid lymphocyte separation tube of this utility model has high applicability and can meet a variety of application needs. Attached Figure Description

[0020] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0021] Figure 1 This is a schematic diagram of the structure of a volume-adjustable rapid lymphocyte separation tube according to this utility model;

[0022] Figure 2 This is a schematic diagram of the separation device of a volume-adjustable rapid lymphocyte separation tube according to the present invention.

[0023] Figure 3 This is a bottom view of the separation device of a volume-adjustable rapid lymphocyte separation tube according to this utility model.

[0024] Figure 4 This is a top view of the separation device of a volume-adjustable rapid lymphocyte separation tube according to this utility model;

[0025] Figure 5 This is a schematic diagram of the rotating rod of a lymphocyte rapid separation tube with adjustable volume according to this utility model;

[0026] Figure 6 This is a utility model Figure 3 A magnified view of A in the middle.

[0027] The reference numerals in the attached figures are:

[0028] 1-tube body; 11-tube cover;

[0029] 2-Separation device; 21-Top plate; 22-Annular barrel section; 23-Central separation hole; 24-Slot hole; 25-Box body;

[0030] 3-Threaded section;

[0031] 4-Rotating rod; 41-First long handle; 411-Snap-fit ​​part; 42-First handle; 43-Second long handle; 44-Second handle;

[0032] 5-Fixing device; 51-Elliptical part; 52-Rotating shaft; 53-Abutting device; 54-Sliding rod; 55-Arc-shaped joint; 56-Sleeve; 57-Limiting ring; 58-Elastic element; 59-Support rod. Detailed Implementation

[0033] The following is in conjunction with the appendix Figures 1-6 The present invention will be further described in detail below. Examples of embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar components or components having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0034] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection or a detachable connection; a mechanical connection or an electrical connection; a direct connection or an indirect connection through an intermediate medium; or the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0035] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used solely for ease of description and simplification of operation, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" are merely used for descriptive distinction and have no special meaning. All parts and equipment use conventional models found in the prior art, and the circuit connections employ conventional connection methods found in the prior art, which will not be detailed here. Content not described in detail in this specification belongs to prior art known to those skilled in the art.

[0036] like Figure 1 As shown, it includes: a tube body 1, a separating device 2, a threaded section 3, and a rotating rod 4. The threaded section 3 is formed on the inner wall of the middle part of the tube body 1. The separating device 2 is slidably connected to the inside of the tube body 1 through the threaded section 3. The separating device 2 has a central separation hole 23 for the sample to pass through during centrifugation. The rotating rod 4 is engaged with the separating device 2 through the central separation hole 23. The outer wall of the tube body 1 is provided with graduations, and the tube body 1 is made of transparent material.

[0037] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the separating device 2 further includes a top plate 21 and an annular barrel portion 22. The top plate 21 has a concave structure, and the annular barrel portion 22 is disposed at the lower part of the top plate 21. The annular barrel portion 22 and the top plate 21 are integrally formed. The outer wall of the annular barrel portion 22 is provided with threads that mate with the threaded section 3. The central separating hole 23 is located in the middle of the top plate 21. The central separating hole 23 has a regular hexagonal structure.

[0038] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the separating device 2 also includes slots 24, which are semi-circular. There are several slots 24, which are arranged around the edge of the top plate 21. The slots 24 connect the upper and lower parts of the top plate 21.

[0039] like Figure 5 As shown, the rotating rod 4 includes a first long handle 41 and a first handle 42. One end of the first long handle 41 is fixedly connected to a snap-fit ​​part 411 that matches the shape of the central separation hole 23, and the other end is fixedly connected to the first handle 42. The long handle and the handle are both integrally formed structures.

[0040] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, it also includes a fixing device 5, which is installed on the lower part of the top plate 21. The side of the annular barrel 22 has a notch for the end of the fixing device 5 to pass through. The end passes through the annular barrel 22 and abuts against the inner wall of the tube body 1. The rotating rod 4 also includes a second long handle 43 and a second handle 44. The middle part of the first long handle 41 has a through hole. The end of the second long handle 43 is fixedly connected to the second handle 44. The second long handle 43 passes through the through hole and is engaged with the fixing device 5.

[0041] like Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, the fixing device 5 includes an elliptical part 51, a rotating shaft 52, and an abutting device 53. A box body 25 is fixedly connected to the lower part of the top plate 21. The two sides of the box body 25 have notches for the ends of the fixing device 5 to pass through. The sides and bottom of the box body 25 have several through holes for the flow and exchange of substances, but without affecting the support of the elliptical part 51. The box body 25 is located below the central separation hole 23 and has a hollow structure. The bottom of the box body 25 is rotatably connected to the elliptical part 51 through the rotating shaft 52. The top of the elliptical part 51 has a snap-fit ​​interface that matches the shape of the second long handle 43. The abutting device 53 is movably connected to both sides of the box body 25, with one end passing through the side wall of the box body 25 and abutting against the elliptical part 51, and the other end passing through the annular barrel part 22 and abutting against the inner wall of the tube body 1.

[0042] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 6 As shown, the abutting device 53 includes: a sliding rod 54, an arc-shaped connector 55, a sleeve 56, a limiting ring 57, an elastic element 58, and a support rod 59. One end of the sliding rod 54 is fixedly connected to the arc-shaped connector 55 through an integrally formed structure. The arc-shaped connector 55 abuts against the elliptical portion 51. The sleeve 56 is sleeved on the outside of the sliding rod 54. The sleeve 56 is fixedly connected to the lower part of the top plate 21 through the support rod 59. The inner wall of the sleeve 56 and the outer wall of the sliding rod 54 are both fixedly connected to the limiting ring 57. The elastic element 58 is sleeved on the outside of the sliding rod 54, and the elastic element 58 is located between the limiting rings 57.

[0043] The working principle of this novel adjustable-volume rapid lymphocyte separation tube is as follows:

[0044] Weigh the blood and calculate the required volume of lymphocyte separation solution (lymphocyte separation solution volume = blood volume * 2). Open the tube cap 11 and insert the thin end of the transfer rod 4 into the central separation hole 23 of the separator 2. Rotate the handle to adjust the separator 2 to the volume mark specified for the lymphocyte separation solution. Use the second handle 42 to control the fixing device 5 to fix the separator 2. Remove the transfer rod 4 and add the lymphocyte separation solution into the tube 1 through the central separation hole 23. Add physiological saline to the blood at a 1:1 ratio, mix well, and then add the blood to be separated along the inner edge of the tube 1. Then close the tube cap 11. After balancing, centrifuge the tube. After centrifugation, blood, red blood cells, and other impurities will fall below the top plate 21; lymphocytes will remain above the top plate 21. Gently pour the liquid above the separator 2 into the centrifuge tube for the next step of preparation. At this point, because the central separation hole 23 is small, the liquid below the central separation hole 23 will not flow out due to surface tension.

[0045] The above are all preferred embodiments of this utility model, and are not intended to limit the scope of protection of this utility model. Therefore, all equivalent changes made to the structure, shape and principle of this utility model should be covered within the scope of protection of this utility model.

Claims

1. A volume-adjustable rapid lymphocyte separation tube, characterized in that, include: The tube (1), the separator (2), the threaded section (3) and the rotating rod (4) are provided. The inner wall of the middle part of the tube (1) is provided with the threaded section (3). The separator (2) is slidably connected to the inside of the tube (1) through the threaded section (3). The separator (2) is provided with a central separation hole (23) for passing the sample through centrifugation. The rotating rod (4) is engaged with the separator (2) through the central separation hole (23).

2. The volume-adjustable rapid lymphocyte separation tube according to claim 1, characterized in that, The separating device (2) further includes a top plate (21) and an annular barrel (22). The top plate (21) has a concave structure. The annular barrel (22) is located at the lower part of the top plate (21). The outer wall of the annular barrel (22) is provided with a thread that is connected to the threaded section (3). The central separating hole (23) is located in the middle of the top plate (21).

3. The volume-adjustable rapid lymphocyte separation tube according to claim 2, characterized in that, The separating device (2) also includes slots (24), which are semi-circular and there are several slots (24) arranged around the edge of the top plate (21).

4. The volume-adjustable rapid lymphocyte separation tube according to claim 2, characterized in that, The rotating rod (4) includes a first long handle (41) and a first handle (42). One end of the first long handle (41) is fixedly connected to a snap-fit ​​part (411) that is adapted to the shape of the central separation hole (23), and the other end is fixedly connected to the first handle (42).

5. The volume-adjustable rapid lymphocyte separation tube according to claim 4, characterized in that, It also includes a fixing device (5), which is installed on the lower part of the top plate (21) and its end passes through the annular barrel part (22) and abuts against the inner wall of the tube body (1). The rotating rod (4) also includes a second long handle (43) and a second handle (44). The first long handle (41) has a through hole in the middle, and the second long handle (44) is fixedly connected to the end of the second long handle (43). The second long handle (43) passes through the through hole and is engaged with the fixing device (5).

6. The volume-adjustable rapid lymphocyte separation tube according to claim 5, characterized in that, The fixing device (5) includes an elliptical part (51), a rotating shaft (52), and an abutting device (53). The lower part of the top plate (21) is fixedly connected to a box body (25). The box body (25) is located below the central separation hole (23) and has a hollow structure. The bottom of the box body (25) is rotatably connected to the elliptical part (51) through the rotating shaft (52). The abutting device (53) is movably connected to both sides of the box body (25), with one end passing through the side wall of the box body (25) and abutting against the elliptical part (51), and the other end passing through the annular barrel part (22) and abutting against the inner wall of the tube body (1).

7. The volume-adjustable rapid lymphocyte separation tube according to claim 6, characterized in that, The abutting device (53) includes: a slide rod (54), an arc-shaped connector (55), a sleeve (56), a limiting ring (57), an elastic element (58), and a support rod (59). One end of the slide rod (54) is fixedly connected to the arc-shaped connector (55), and the arc-shaped connector (55) abuts against the elliptical part (51). The sleeve (56) is sleeved on the outside of the slide rod (54). The sleeve (56) is fixedly connected to the lower part of the top plate (21) through the support rod (59). The inner wall of the sleeve (56) and the outer wall of the slide rod (54) are both fixedly connected to the limiting ring (57). The elastic element (58) is sleeved on the outside of the slide rod (54), and the elastic element (58) is located between the limiting rings (57).

Images