Food enzyme preparation purification equipment

Abstract

The utility model discloses food enzyme preparation purification equipment. The food enzyme preparation purification equipment comprises a pretreatment unit, a membrane separation unit and a chromatographic purification unit, the pretreatment unit adopts a double-layer screen vibrating screen and air flotation centrifugal linkage structure, and efficient preliminary impurity removal is achieved in cooperation with a pH adjusting system. And the membrane separation unit is combined with the tubular ceramic module and the hollow fiber module, so that the membrane separation unit has excellent anti-pollution performance and online monitoring capability. The chromatographic purification unit adopts a modular chromatographic column array and carries a buffer solution gradient generator and a multi-parameter detection system, so that the extraction precision and efficiency of target components are improved. The device is high in structural integration level, high in automation level and suitable for efficient and stable purification of the food enzyme preparation.

Description

Technical Field

[0001] This utility model relates to equipment for purifying food enzyme preparations. Background Technology

[0002] Food enzyme preparations are widely used in food processing, fermentation, brewing, preservation, and other fields. Their purity and activity directly affect their functional performance in the final product. Traditional enzyme purification methods typically include crude extraction, centrifugation, filtration, membrane separation, and chromatography. However, existing equipment still faces many problems in practical applications:

[0003] 1. The pretreatment process is inefficient, such as incomplete separation by screening and centrifugation, resulting in residual impurities;

[0004] 2. The membrane separation system suffers from severe fouling and weak backwashing capacity, leading to frequent membrane fouling and poor operational stability;

[0005] 3. Chromatographic purification steps have low modularity, are cumbersome to operate, and are difficult to replace consumables and maintain;

[0006] 4. The overall level of automation and online monitoring is limited, which affects the stability and repeatability of purification.

[0007] Therefore, there is an urgent need to develop a food enzyme preparation purification equipment that is structurally integrated, easy to operate, highly efficient in purification, and has strong online detection capabilities, in order to improve the quality of the preparations and production efficiency. Utility Model Content

[0008] The purpose of this invention is to overcome the shortcomings of the prior art and provide a food enzyme preparation purification device that is easy to connect.

[0009] Food enzyme preparation purification equipment includes a pretreatment unit, a membrane separation unit, and a chromatography purification unit. The pretreatment unit includes a vibrating screen assembly and an air flotation centrifuge unit. The vibrating screen assembly includes a double-layer screen made of 304 stainless steel, connected to an eccentric wheel vibration motor. A liquid collection tank with a slope ≥15° is located at the bottom of the screen to guide the liquid to the air flotation centrifuge unit. The bottom of the air flotation centrifuge unit is directly connected to a pH adjustment tank, which contains acid / alkali spray nozzles and a pH probe. The pH adjustment tank is connected to the pretreatment tank, which is connected to the membrane separation unit via a pretreatment liquid pump. The unit includes a tubular ceramic module and a hollow fiber module. The inner wall of the flow channel of the tubular ceramic membrane is mirror-polished. The hollow fiber module is installed at a 15° angle and equipped with a pulse backflush system. A turbidity sensor is installed on the permeate side of the hollow fiber module. The membrane separation unit is connected to the chromatography purification unit. The chromatography purification unit includes a modular chromatography column array and is equipped with a buffer gradient generator. The chromatography column array adopts a quick-connect column, and the column head distributor adopts a porous titanium plate. The modular chromatography column array is connected to the detection system, which includes an ultraviolet detection flow cell and a conductivity meter. The liquid that reaches the target parameters enters the collection tank, and the waste liquid enters the recovery tank.

[0010] Three-stage integrated design: The series structure of pretreatment → membrane separation → chromatography enables continuous production and reduces intermediate material transfer losses;

[0011] 304 stainless steel double-layer screen: corrosion resistant and meets food hygiene standards. The upper corrugated screen plate design can prevent fiber entanglement, and the lower perforated screen improves the interception rate of fine particles.

[0012] Direct connection of air flotation centrifuge unit to pH adjustment tank: avoids secondary contamination of bacteria, and dissolved air microbubbles improve the removal efficiency of lipid impurities;

[0013] Ceramic membrane mirror polishing + hollow fiber membrane tilted installation: reduces membrane surface adsorption, and the tilted layout combined with pulse backwashing effectively alleviates membrane fouling;

[0014] Quick-connect chromatography column + porous titanium plate distributor: shortens column equilibration time, titanium material is corrosion resistant and has uniform flow distribution, improving chromatography resolution;

[0015] UV + conductivity dual detection system: Real-time monitoring of target enzyme activity peaks to ensure collection purity.

[0016] As a further improvement, the upper layer of the double-layer screen is a corrugated screen plate with a pore size of 100μm, and the lower layer is a perforated screen with a pore size of 50μm. The 100μm corrugated screen intercepts large particles, and the 50μm perforated screen removes bacterial fragments, thereby improving the combined filtration efficiency.

[0017] As a further improvement, the eccentric wheel vibrating motor is an adjustable motor with an amplitude of 2-5mm and a vibration frequency of 20-50Hz, which can adapt to materials of different viscosities and optimize screening efficiency; it is suitable for materials that are prone to foaming and reduces liquid splashing.

[0018] As a further improvement, the air flotation centrifuge unit includes a disc-type centrifugal drum and a built-in dissolved gas release device, which generates microbubbles to adsorb fat-soluble impurities, thus solving the problem of insufficient separation of light impurities by traditional centrifuges.

[0019] As a further improvement, the pulse backflush system has a backflush pressure of 0.8-1.2 MPa and a cycle of 5 min / time, which effectively removes the fouling layer on the membrane surface, extends membrane life, maintains stable membrane flux, and avoids energy waste caused by continuous backflush.

[0020] As a further improvement, the tubular ceramic module includes a 19-channel tubular ceramic membrane, with 6 tubular ceramic membranes connected in parallel to form a group, increasing the effective filtration area and improving the throughput. The parallel flow channels reduce the load on a single membrane and reduce pressure drop fluctuations.

[0021] Beneficial effects:

[0022] 1. High-efficiency pretreatment design: It adopts a double-layer screen structure made of 304 stainless steel, with different apertures set in the upper and lower layers to achieve multi-stage screening; combined with an eccentric wheel vibration motor with adjustable frequency and amplitude, it improves screening efficiency and reduces clogging; the liquid collection tank is set with a slope of ≥15° to quickly guide the flow and avoid liquid retention.

[0023] 2. Deep solid-liquid separation: The system incorporates an air flotation centrifugation linkage system and uses a disc-type rotating drum and dissolved gas release device to effectively remove suspended impurities and foam, thereby enhancing the centrifugation impurity removal efficiency.

[0024] 3. Intelligent pH adjustment system: The pH adjustment tank with built-in acid and alkali spray nozzles and pH probe ensures that the material is in the optimal pH condition before entering the membrane system, thereby improving the subsequent separation effect.

[0025] 4. Advanced membrane separation module: Combining tubular ceramic membrane and hollow fiber membrane, the ceramic membrane has a mirror-like inner wall to improve flowability and fouling resistance, while the hollow fiber membrane is installed at an angle and equipped with a pulse backwash system to enhance its anti-fouling and self-cleaning capabilities. A turbidity sensor is installed at the permeate end to enable online monitoring.

[0026] 5. Modular Chromatography Purification System: It adopts a quick-connect column structure and a porous titanium plate distributor, combined with a buffer gradient generator and a multi-parameter detection system, to achieve automatic identification of separation segments and accurate collection of target components, thereby improving purification accuracy.

[0027] 6. Automatic control and waste liquid management: The system is equipped with collection tanks and waste liquid recovery tanks at the end, and in conjunction with online detection devices, it can achieve automatic diversion, improve the degree of automation and resource recovery rate. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of a food enzyme preparation purification equipment. Detailed Implementation

[0029] To enhance understanding of this utility model, the present utility model will be further described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain the present utility model and do not constitute a limitation on the scope of protection of the present utility model.

[0030] like Figure 1 As shown, the food enzyme preparation purification equipment includes a pretreatment unit, a membrane separation unit, and a chromatography purification unit. The pretreatment unit includes a vibrating screen assembly and an air flotation centrifuge unit. The vibrating screen assembly includes a double-layer screen made of 304 stainless steel, connected to an eccentric wheel vibration motor. A liquid collection tank with a slope ≥15° is located at the bottom of the screen, guiding the liquid to the air flotation centrifuge unit. The bottom of the air flotation centrifuge unit is directly connected to a pH adjustment tank, which contains acid / alkali spray nozzles and a pH probe. The pH adjustment tank is connected to the pretreatment tank, which is connected to the membrane separation unit via a pretreatment liquid pump. The separation unit includes a tubular ceramic module and a hollow fiber module. The inner wall of the flow channel of the tubular ceramic membrane is mirror-polished. The hollow fiber module is installed at a 15° angle and equipped with a pulse backflush system. A turbidity sensor is installed on the permeate side of the hollow fiber module. The membrane separation unit is connected to the chromatography purification unit. The chromatography purification unit includes a modular chromatography column array and is equipped with a buffer gradient generator. The chromatography column array uses quick-connect columns, and the column head distributor uses a porous titanium plate. The modular chromatography column array is connected to the detection system, which includes an ultraviolet detection flow cell and a conductivity meter. Liquid that meets the target parameters enters the collection tank, and waste liquid enters the recovery tank.

[0031] Three-stage integrated design: The series structure of pretreatment → membrane separation → chromatography enables continuous production and reduces intermediate material transfer losses;

[0032] 304 stainless steel double-layer screen: corrosion resistant and meets food hygiene standards. The upper corrugated screen plate design can prevent fiber entanglement, and the lower perforated screen improves the interception rate of fine particles.

[0033] Direct connection of air flotation centrifuge unit to pH adjustment tank: avoids secondary contamination of bacteria, and dissolved air microbubbles improve the removal efficiency of lipid impurities;

[0034] Ceramic membrane mirror polishing + hollow fiber membrane tilted installation: reduces membrane surface adsorption, and the tilted layout combined with pulse backwashing effectively alleviates membrane fouling;

[0035] Quick-connect chromatography column + porous titanium plate distributor: shortens column equilibration time, titanium material is corrosion resistant and has uniform flow distribution, improving chromatography resolution;

[0036] UV + conductivity dual detection system: Real-time monitoring of target enzyme activity peaks to ensure collection purity.

[0037] The upper layer of the double-layer screen is a corrugated screen plate with a pore size of 100μm, and the lower layer is a perforated screen with a pore size of 50μm. The 100μm corrugated screen intercepts large particles, and the 50μm perforated screen removes bacterial fragments, thus improving the combined filtration efficiency.

[0038] The eccentric wheel vibrating motor is an adjustable motor with an amplitude of 2-5mm and a vibration frequency of 20-50Hz, which is suitable for materials of different viscosities and optimizes screening efficiency; it is also suitable for materials that are prone to foaming and reduces liquid splashing.

[0039] The air flotation centrifuge unit includes a disc-type centrifugal drum with a built-in dissolved gas release device, which generates microbubbles to adsorb fat-soluble impurities, solving the problem of insufficient separation of light impurities by traditional centrifuges.

[0040] The pulse backflush system has a backflush pressure of 0.8-1.2 MPa and a cycle of 5 min / time, which effectively removes the fouling layer on the membrane surface, extends membrane life, maintains stable membrane flux, and avoids energy waste caused by continuous backflush.

[0041] The tubular ceramic module includes a 19-channel tubular ceramic membrane, with 6 tubular ceramic membranes connected in parallel to increase the effective filtration area and improve the throughput. The parallel flow channels reduce the load on a single membrane and reduce pressure drop fluctuations.

[0042] Overall workflow of the device:

[0043] 1. Preprocessing stage:

[0044] Vibrating screen: used for grading and removing solid impurities;

[0045] Air flotation centrifugation: dissolved air microbubbles adsorb lipids + high-speed centrifugation removes bacterial cells;

[0046] pH adjustment: Precisely control the optimal pH for enzymes;

[0047] 2. Membrane separation stage:

[0048] Ceramic membranes: retain large molecular proteins while allowing target enzymes to pass through;

[0049] Hollow fiber membrane: Concentrates target enzymes and removes small molecule impurities;

[0050] 3. Chromatographic purification stage:

[0051] Gradient elution: selective separation of isoenzymes via ion exchange;

[0052] Online detection: Automatic collection triggered by dual parameters of ultraviolet absorption and conductivity.

[0053] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. Food enzyme preparation purification equipment, characterized in that, The system includes a pretreatment unit, a membrane separation unit, and a chromatography purification unit. The pretreatment unit comprises a vibrating screen assembly and an air flotation centrifuge unit. The vibrating screen assembly includes a double-layer screen made of 304 stainless steel, connected to an eccentric wheel vibrating motor. A collection tank with a slope ≥15° is located at the bottom of the screen to guide the liquid to the air flotation centrifuge unit. The bottom of the air flotation centrifuge unit is directly connected to a pH adjustment tank, which contains acid / alkali spray nozzles and a pH probe. The pH adjustment tank is connected to the pretreatment tank, which is connected to the membrane separation unit via a pretreatment liquid pump. The membrane separation unit includes a tubular... The system includes ceramic and hollow fiber modules. The inner wall of the tubular ceramic membrane flow channel is mirror-polished. The hollow fiber module is installed at a 15° angle and equipped with a pulse backflush system. A turbidity sensor is installed on the permeate side of the hollow fiber module. The membrane separation unit is connected to the chromatography purification unit, which includes a modular chromatography column array and is equipped with a buffer gradient generator. The chromatography column array uses quick-connect columns, and the column head distributor uses a porous titanium plate. The modular chromatography column array is connected to a detection system, which includes an ultraviolet detection flow cell and a conductivity meter. Liquids that meet the target parameters enter a collection tank, and waste liquid enters a recovery tank.

2. The food enzyme preparation purification equipment according to claim 1, characterized in that, The upper layer of the double-layer screen is a corrugated screen plate with a pore size of 100μm, and the lower layer is a perforated screen with a pore size of 50μm.

3. The food enzyme preparation purification equipment according to claim 1, characterized in that, The eccentric wheel vibration motor is an adjustable motor with an amplitude of 2-5mm and a vibration frequency of 20-50Hz.

4. The food enzyme preparation purification equipment according to claim 1, characterized in that, The aforementioned air flotation centrifuge unit includes a disc-type centrifugal drum and a built-in dissolved gas release device.

5. The food enzyme preparation purification equipment according to claim 1, characterized in that, The pulse recoil system has a recoil pressure of 0.8-1.2 MPa and a cycle of 5 min / cycle.

6. The food enzyme preparation purification equipment according to claim 1, characterized in that, The tubular ceramic module includes a 19-channel tubular ceramic membrane, with 6 tubular ceramic membranes connected in parallel to form a group.

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