Microorganism sample digestion dilution and inoculation scribing device

Abstract

The utility model provides a microbiological sample digestion dilution and inoculation lineation device, which solves the problems of single function and large occupied area of the existing microbiological treatment device. Comprising a workbench; a first rack and a second rack which are perpendicular to each other are arranged on the workbench, a test tube carrying mechanism is arranged on the first rack in a sliding manner, and a pipetting pump mechanism, a culture dish lineation mechanism and a culture dish carrying mechanism are sequentially arranged on the second rack in a sliding manner; a test tube placing rack is arranged on the adjacent side of the first rack, a digestive juice storage cup is arranged below the pipetting pump mechanism, a test tube clamping mechanism which is in sliding connection with the workbench is arranged between the test tube placing rack and the digestive juice storage cup, and the test tube clamping mechanism is respectively matched with the test tube carrying mechanism and the pipetting pump mechanism; a culture dish push-out mechanism is arranged on the adjacent side of the test tube placing rack and is matched with the culture dish lineation mechanism and the culture dish carrying mechanism respectively. The device is widely applied to the technical field of microbial instruments.

Description

Technical Field

[0001] This application relates to the field of microbial instrument technology, and more specifically, to a device for digesting, diluting, and streaking microbial samples. Background Technology

[0002] In the field of microbiological testing, sputum and urine sample analysis are crucial for preoperative diagnosis and disease screening. Sputum exhibits significant heterogeneity, with uneven distribution and concentration of bacteria and other components, severely impacting the accuracy of test results. Clinically, sputum samples typically require pretreatment before testing. Traditional methods involve manually adding an equal volume of digestive fluid followed by incubation or shaking. However, manual methods suffer from significant dosage control errors, poor mixing effects, and low efficiency.

[0003] Currently, some automated testing equipment has emerged on the market. Through pipetting pumps and shaking mechanisms, it achieves quantitative addition and mixing of digestive fluids, solving the problems mentioned above in sputum testing. However, in practical use, the following issues still exist: existing sample testing equipment has limited functionality; urine testing equipment lacks a corresponding digestive fluid addition structure, failing to meet the pre-dilution requirement for sputum testing. Therefore, urine and sputum testing are performed using different equipment, resulting in laboratories needing multiple independent devices. This leads to high equipment manufacturing costs, large footprint, and low space utilization. Utility Model Content

[0004] To address the aforementioned problems, the present invention provides a microbial sample digestion, dilution, and inoculation streaking device, solving the issues of limited functionality and large footprint of existing microbial processing devices. The device includes a workbench; a first frame and a second frame are perpendicularly distributed on the workbench. A test tube transport mechanism slides on the first frame, and a pipette pump mechanism, a petri dish streaking mechanism, and a petri dish transport mechanism slide sequentially on the second frame. A test tube rack is located adjacent to the first frame, and a digestion solution storage cup is located below the pipette pump mechanism. A test tube clamping mechanism, slidably connected to the workbench, is located between the test tube rack and the digestion solution storage cup, cooperating with both the test tube transport mechanism and the pipette pump mechanism. A petri dish ejection mechanism is located adjacent to the test tube rack, cooperating with both the petri dish streaking mechanism and the petri dish transport mechanism.

[0005] Preferably, a barcode scanner is provided between the test tube rack and the test tube clamping mechanism; the test tube clamping mechanism includes a linear displacement component and a clamping component set on the worktable, the clamping component includes a clamping base plate connected to the moving end of the linear displacement component, a clamping bracket set on the surface of the clamping base plate and a weighing platform, the clamping bracket is provided with a clamping device that cooperates with the weighing platform to clamp the test tubes, and the weighing platform is provided with a weight sensor for weighing the test tubes.

[0006] Preferably, a label printing and pasting mechanism and a culture dish storage mechanism for storing processed culture dishes are sequentially provided on the side adjacent to the culture dish ejection mechanism along the length of the second frame. The culture dish transport mechanism cooperates with the label printing and pasting mechanism and the culture dish storage mechanism to label and store the bottom of the transported culture dishes.

[0007] Preferably, the petri dish storage mechanism includes a conveying component, a lifting component, and a petri dish storage rack for placing processed petri dishes on a worktable. The conveying component includes a conveying platform that cooperates with the petri dish handling mechanism to place the petri dishes and a conveying drive unit disposed on the conveying platform. The surface of the conveying platform is provided with a conveying chute, and the end of the conveying chute is provided with a circular groove. The output end of the conveying drive unit is provided with a conveying push plate that cooperates with the conveying chute. The conveying push plate pushes the petri dishes along the surface of the conveying platform to the circular groove. The lifting component includes a lifting drive unit disposed below the petri dish storage rack. The output end of the lifting drive unit is provided with a circular tray. The circular tray cooperates with the circular groove to push the petri dishes upward into the petri dish storage rack.

[0008] Preferably, the test tube transport mechanism includes an X-axis displacement component slidably connected to the first frame and a fourth Z-axis displacement component disposed on the moving end of the X-axis displacement component. The moving end of the fourth Z-axis displacement component is provided with a gripper. The gripper cooperates with the test tube placement rack to grip the test tube and cooperates with the test tube clamping mechanism to open the cap of the gripped test tube. A first displacement drive component is provided on the first frame, and the output end of the first displacement drive component is connected to the X-axis displacement component.

[0009] Preferably, the pipetting pump mechanism includes a first Y-axis displacement assembly slidably connected to the second frame and a first Z-axis displacement assembly disposed on the moving end of the first Y-axis displacement assembly, wherein the moving end of the first Z-axis displacement assembly is provided with a pipetting pump.

[0010] The petri dish streaking mechanism includes a second Y-axis displacement component slidably connected to the second frame and a second Z-axis displacement component disposed on the moving end of the second Y-axis displacement component. The moving end of the second Z-axis displacement component is provided with an inoculation ring. The inoculation ring cooperates with the test tube on the test tube clamping mechanism to streak the petri dish on the petri dish ejection mechanism.

[0011] The petri dish transport mechanism includes a connecting bracket that is slidably connected to the second frame and a third Z-axis displacement assembly disposed at the end of the connecting bracket. The moving end of the third Z-axis displacement assembly is provided with a gripper for transporting the petri dish.

[0012] The second frame is equipped with a second displacement drive assembly, and the output end of the second displacement drive assembly is connected to the first Y-axis displacement assembly, the second Y-axis displacement assembly and the connecting bracket respectively.

[0013] Preferably, the petri dish ejection mechanism includes a lifting component, a pushing and displacing component, and a petri dish rack for placing untreated petri dishes on a worktable. The lifting component is located below the petri dish rack. The moving end of the pushing and displacing component is provided with a pushing plate. A pushing platform is provided above the pushing and displacing component. The pushing platform is provided with a fixing component and a pushing groove that cooperates with the pushing plate. The lifting component and the fixing component cooperate to fix part of the petri dishes. The pushing plate pushes the petri dish at the bottom of the petri dish rack to slide along the pushing platform to the end of the pushing groove. A storage slot is provided at the end of the pushing groove. A rotating component that drives the petri dishes to rotate is provided below the storage slot. A petri dish marking mechanism cooperates with the storage slot to mark the petri dishes.

[0014] Preferably, the bottom of the test tube rack is provided with a test tube oscillation mechanism, which includes a mounting plate set on the bottom surface of the workbench and an oscillation motor vertically set on the bottom surface of the mounting plate. The output end of the oscillation motor is connected to an eccentric shaft, which is rotatably connected to the test tube rack.

[0015] Preferably, a pipette holder is provided on the side adjacent to the digestive fluid storage cup, and the pipette holder is provided with a plurality of pipettes that cooperate with the pipette pump mechanism.

[0016] Preferably, an infrared sterilizer is provided between the petri dish streaking mechanism and the label printing and pasting mechanism.

[0017] The beneficial effects of this invention are as follows: This device can detect urine and sputum samples separately. Through the cooperation of the test tube clamping mechanism and other mechanisms, it achieves an integrated process of direct urine detection and pre-diluted sputum detection, simplifying the overall structure of the device, reducing manufacturing costs, and improving the functional integration and detection efficiency. The first and second frames are perpendicularly distributed, with each mechanism arranged along the length of the second frame, resulting in a compact layout that fully utilizes the workbench surface space, optimizes the working paths of each mechanism on the second frame, improves sample detection efficiency, and reduces the equipment's footprint. The device has a high degree of automation, automating the entire process from test tube handling, opening, digestion fluid aspiration, addition, and shaking, to petri dish opening, streaking, handling, labeling, and storage. This reduces manual labor intensity and operational errors, ensures consistency in sample processing, and improves the accuracy and reliability of test results. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a top view of the present invention;

[0021] Figure 3 This is a schematic diagram of the test tube clamping mechanism;

[0022] Figure 4 This is a schematic diagram of the structure of the first frame;

[0023] Figure 5 This is a schematic diagram of the second frame;

[0024] Figure 6 This is a schematic diagram of the structure of a petri dish storage mechanism;

[0025] Figure 7 This is a schematic diagram of the test tube oscillation mechanism;

[0026] Symbols in the diagram: 1. Workbench; 2. First frame; 3. Second frame; 4. Test tube handling mechanism; 5. Pipette pump mechanism; 6. Petri dish streaking mechanism; 7. Petri dish handling mechanism; 8. Test tube rack; 9. Digestive fluid storage cup; 10. Test tube clamping mechanism; 11. Petri dish ejection mechanism; 12. Barcode scanner; 13. Label printing and pasting mechanism; 14. Petri dish storage mechanism; 15. Test tube shaking mechanism; 16. Pipette rack; 17. Infrared sterilizer; 21. First displacement drive assembly; 31. Second displacement drive assembly; 41. X-axis displacement assembly; 42. Fourth Z-axis displacement assembly; 43. Gripper; 51. First Y-axis displacement assembly; 52. First Z-axis displacement assembly; 5 3. Pipette pump; 61. Second Y-axis displacement assembly; 62. Second Z-axis displacement assembly; 63. Inoculation loop; 71. Connecting bracket; 72. Third Z-axis displacement assembly; 73. Gripper; 101. Linear displacement assembly; 102. Clamping assembly; 1021. Clamping base plate; 1022. Clamping bracket; 1023. Weighing platform; 1024. Clamping device; 141. Conveying assembly; 142. Lifting assembly; 143. Petri dish storage rack; 1411. Conveying platform; 1412. Conveying drive unit; 1413. Conveying chute; 1414. Circular groove; 1415. Conveying push plate; 1421. Lifting drive unit; 1422. Circular tray; 151. Mounting plate; 152. Vibrating motor. Detailed Implementation

[0027] To make the technical problems, technical solutions, and beneficial effects to be solved by this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and are not intended to limit the scope of this application.

[0028] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0029] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0030] The present application will now describe a microbial sample digestion, dilution, and inoculation streak device provided in the embodiments of this application.

[0031] Please see Figure 1 and Figure 2The microbial sample digestion, dilution, and inoculation streaking device includes a workbench 1; the workbench 1 has a first frame 2 and a second frame 3 arranged perpendicularly to each other; a test tube transport mechanism 4 is slidably mounted on the first frame 2; and a pipette pump mechanism 5, a petri dish streaking mechanism 6, and a petri dish transport mechanism 7 are slidably mounted sequentially on the second frame 3. A test tube rack 8 is located adjacent to the first frame 2, and several test tubes are placed on the test tube rack 8; a digestive fluid storage cup 9 is located below the pipette pump mechanism 5, and the digestive fluid storage cup 9 contains digestive fluid for diluting sputum. A test tube clamping mechanism 10, slidably connected to the workbench 1, is provided between the test tube rack 8 and the digestive fluid storage cup 9. The test tube clamping mechanism 10 cooperates with the test tube transport mechanism 4 and the pipetting pump mechanism 5, respectively. Specifically, the test tube transport mechanism 4 picks up the test tube containing the sputum sample from the test tube rack 8 and moves it to the test tube clamping mechanism 10. The test tube clamping mechanism 10 clamps the test tube, and the test tube transport mechanism 4 clamps the test tube cap and moves it upward, completing the opening of the test tube. Subsequently, the test tube clamping mechanism 10 moves the test tube closer to the digestive fluid storage cup 9, and the pipetting pump mechanism 5 starts, drawing digestive fluid from the digestive fluid storage cup 9 and dripping it into the test tube, completing the dilution of the sputum sample. A petri dish ejection mechanism 11 is provided adjacent to the test tube rack 8, and the petri dish ejection mechanism 11 cooperates with the petri dish streaking mechanism 6 and the petri dish transport mechanism 7, respectively. Specifically, the petri dish ejection mechanism 11 ejects a single untreated petri dish, the petri dish transport mechanism 7 first starts to open the petri dish, then the petri dish streaking mechanism 6 starts, dips into the sample in the test tube and moves it above the petri dish to streak it, the petri dish transport mechanism 7 starts again, slides along the second frame 3, and transports the streaked petri dish to the next station.

[0032] Please see Figures 1 to 3Furthermore, a barcode scanner 12 is provided between the test tube rack 8 and the test tube clamping mechanism 10. Specifically, when the test tube transport mechanism 4 clamps the test tube to be tested from the test tube rack 8 to the test tube clamping mechanism 10, the barcode scanner 12 automatically scans the barcode or QR code on the test tube, identifies and records the detection information, so as to ensure that subsequent operations correspond to the sample and improve the accuracy and automation of the detection process. The test tube clamping mechanism 10 includes a linear displacement component 101 and a clamping component 102 disposed on the workbench 1. In this embodiment, the linear displacement component 101 is a belt drive mechanism. The belt drive mechanism is existing technology and will not be described in detail here. The clamping assembly 102 includes a clamping base plate 1021 connected to the movable end of the linear displacement assembly 101, a clamping bracket 1022 disposed on the surface of the clamping base plate 1021, and a weighing platform 1023. The clamping bracket 1022 is equipped with a gripper 1024 that cooperates with the weighing platform 1023 to clamp the test tubes. The weighing platform 1023 contains a weight sensor for weighing the test tubes. Specifically, the clamping assembly 102 cooperates with the test tube transport mechanism 4. When a test tube is placed on the weighing platform 1023, the gripper 1024 fixes the test tube and weighs it. The weighing data is transmitted to the control system to determine the amount of digestion solution added by the pipette pump mechanism 5, avoiding over- or under-addition and ensuring the reliability of subsequent test results. The test tube clamping mechanism 10 moves between the test tube rack 8 and the digestive fluid storage cup 9, adapting to two detection procedures: when the sample needs to be diluted before detection, the linear displacement component 101 drives the clamping component 102 and the test tube to approach the digestive fluid storage cup 9 horizontally, cooperating with the pipetting pump mechanism 5 to complete the addition of digestive fluid. After the addition is completed, the linear displacement component 101 drives the clamping component 102 and the test tube to return to their original positions. When the sample needs to be directly detected, the linear displacement component 101 drives the clamping component 102 and the test tube to approach the digestive fluid storage cup 9 horizontally, skipping the digestive fluid dilution step, and cooperating with the petri dish streaking mechanism 6 to complete the sample collection. Through the test tube clamping mechanism 10, this device can realize an integrated process of direct urine detection and sputum dilution before detection, simplifying the overall structure of the device, reducing the manufacturing cost, and improving the functional integration and detection efficiency of the device.

[0033] Please see Figure 1 and Figure 2 In this embodiment, a label printing and pasting mechanism 13 and a culture dish storage mechanism 14 for storing processed culture dishes are sequentially arranged along the length of the second frame 3 adjacent to the culture dish ejection mechanism 11. The culture dish transport mechanism 7 cooperates with the label printing and pasting mechanism 13 and the culture dish storage mechanism 14 to label and store the transported culture dishes. The various mechanisms are arranged along the length of the second frame 3, with a compact layout, making full use of the surface space of the workbench 1, optimizing the working path of each mechanism on the second frame 3, improving the sample detection efficiency, and reducing the equipment footprint.

[0034] Please see Figure 1 , Figure 2 and Figure 4 Specifically, the test tube transport mechanism 4 includes an X-axis displacement component 41 slidably connected to the first frame 2 and a fourth Z-axis displacement component 42 disposed on the moving end of the X-axis displacement component 41. The moving end of the fourth Z-axis displacement component 42 is provided with a gripper 43. The gripper 43 cooperates with the test tube placement rack 8 to grip the test tube and cooperates with the test tube clamping mechanism 10 to open the cap of the gripped test tube. The first frame 2 is provided with a first displacement drive component 21, the output end of which is connected to the X-axis displacement component 41. Specifically, when the first displacement drive component 21 is activated, it drives the X-axis displacement component 41 to slide along the first frame 2, and the fourth Z-axis displacement component 42 and the gripper 43 move to above the test tube placement rack 8. The fourth Z-axis displacement component 42 drives the gripper 43 to clamp the top of the test tube, completing the gripping of the test tube. Subsequently, the first displacement drive component 21 and the X-axis displacement component 41 are activated to transport the test tube to above the test tube clamping mechanism 10. In this embodiment, the first displacement drive component 21 and the X-axis displacement component 41 are belt drive mechanisms, and the fourth Z-axis displacement component 42 is a drive device such as a cylinder or a linear motor.

[0035] Please see Figure 1 , Figure 2 and Figure 5 Specifically, the pipetting pump mechanism 5 includes a first Y-axis displacement assembly 51 slidably connected to the second frame 3 and a first Z-axis displacement assembly 52 disposed on the moving end of the first Y-axis displacement assembly 51. The moving end of the first Z-axis displacement assembly 52 is provided with a pipetting pump 53. When pipetting is required, the first Y-axis displacement assembly 51 slides along the second frame 3, causing the pipetting pump 53 to move above the digestion solution storage cup 9. The first Z-axis displacement assembly 52 drives the pipetting pump 53 to descend and draw a quantitative amount of digestion solution. The first Y-axis displacement assembly 51 drives the first Z-axis displacement assembly 52 to move above the test tube and add digestion solution.

[0036] Specifically, the petri dish streaking mechanism 6 includes a second Y-axis displacement component 61 slidably connected to the second frame 3 and a second Z-axis displacement component 62 disposed on the moving end of the second Y-axis displacement component 61. The moving end of the second Z-axis displacement component 62 is provided with an inoculation loop 63. When streaking is required, the second Y-axis displacement component 61 slides along the second frame 3, driving the second Z-axis displacement component 62 and the inoculation loop 63 to move above the test tube held by the test tube clamping mechanism 10. The second Z-axis displacement component 62 drives the inoculation loop 63 to descend and pick up an appropriate amount of sample. Subsequently, the second Y-axis displacement component 61 drives the inoculation loop 63 to move above the petri dish pushed out by the petri dish ejection mechanism 11. The second Y-axis displacement component 61 and the second Z-axis displacement component 62 control the inoculation loop 63 to streak on the surface of the petri dish.

[0037] Specifically, the petri dish transport mechanism 7 includes a connecting bracket 71 slidably connected to the second frame 3 and a third Z-axis displacement component 72 disposed at the end of the connecting bracket 71. The moving end of the third Z-axis displacement component 72 is provided with a gripper 73 for transporting petri dishes. The connecting bracket 71 slides along the second frame 3, driving the third Z-axis displacement component 72 and the gripper 73 to move above the petri dish ejection mechanism 11. The third Z-axis displacement component 72 descends, and the gripper 73 grasps the petri dish. Subsequently, the petri dish transport mechanism 7 slides along the second frame 3, transporting the petri dish above the label printing and pasting mechanism 13, and lowers it to place the petri dish for labeling. After labeling is completed, the petri dish transport mechanism 7 moves again, transporting the petri dish to the petri dish storage mechanism 14, completing the entire transport process.

[0038] Specifically, the second frame 3 is equipped with a second displacement drive assembly 31, the output end of which is connected to the first Y-axis displacement assembly 51, the second Y-axis displacement assembly 61, and the connecting bracket 71, respectively. In this embodiment, the second displacement drive assembly 31, the first Y-axis displacement assembly 51, and the second Y-axis displacement assembly 61 are belt drive mechanisms, and the first Z-axis displacement assembly 52, the second Z-axis displacement assembly 62, and the third Z-axis displacement assembly 72 are drive devices such as cylinders or linear motors.

[0039] Specifically, the petri dish ejection mechanism 11 includes a lifting component, a pushing and displacing component, and a petri dish rack for placing untreated petri dishes, all mounted on the worktable 1. Several untreated petri dishes are stacked on the rack. The lifting component is located below the rack. The moving end of the pushing and displacing component has a pushing plate, and a pushing platform is located above the pushing and displacing component. The pushing platform has a fixing component and a pushing groove that cooperates with the pushing plate. The lifting component and the fixing component cooperate to fix some of the petri dishes. The pushing plate pushes the bottommost petri dish of the rack along the pushing platform to the end of the pushing groove. A storage slot is located at the end of the pushing groove, and a rotating component that drives the petri dishes to rotate is located below the storage slot. The petri dish marking mechanism 6 cooperates with the storage slot to mark lines on the petri dishes. When a petri dish needs to be ejected, the lifting component moves upward, raising the stacked petri dishes on the rack until the second-to-last petri dish is aligned with the fixing component. The fixing component then activates, fixing the second-to-last petri dish and the petri dishes above it. At this time, the push-displacement component is activated, and the push plate drives the lowest culture dish of the culture dish placement rack to slide along the push platform. After the culture dish is pushed into the storage tank, the culture dish streaking mechanism 6 moves above the storage tank and uses the inoculation loop 63 to pick up the sample and streak the culture dish in the storage tank. After the streaking operation is completed, the rotation component drives the culture dish in the storage tank to rotate to an angle that is easy to observe and handle. In this embodiment, the lifting component, fixing component, and push-displacement component are existing technologies and will not be described in detail here.

[0040] Please see Figure 1, Figure 2 and Figure 6 Furthermore, the petri dish storage mechanism 14 includes a conveying assembly 141, a lifting assembly 142, and a petri dish storage rack 143 for placing processed petri dishes, all mounted on the workbench 1. The conveying assembly 141 includes a conveying platform 1411 that cooperates with the petri dish transport mechanism 7 to place petri dishes and a conveying drive unit 1412 mounted on the conveying platform 1411. The surface of the conveying platform 1411 is provided with a conveying chute 1413, and the end of the conveying chute 1413 is provided with a circular groove 1414. The output end of the conveying drive unit 1412 is provided with a conveying push plate 1415 that cooperates with the conveying chute 1413. The conveying push plate 1415 pushes the petri dish along the surface of the conveying platform 1411 to the circular groove 1414. The lifting assembly 142 includes a lifting drive unit 1421 located below the petri dish storage rack 143. The output end of the lifting drive unit 1421 is provided with a circular tray 1422. The circular tray 1422 cooperates with the circular groove 1414 to push the petri dish upward into the petri dish storage rack 143. Specifically, the petri dish transport mechanism 7 places the labeled petri dishes onto the conveyor platform 1411. The conveyor drive unit 1412 drives the conveyor pusher plate 1415 to slide along the conveyor chute 1413. The conveyor pusher plate 1415 pushes the petri dishes into the circular groove 1414. The lifting drive unit 1421 is activated, lifting the petri dishes upwards via the circular tray 1422, allowing them to enter the petri dish storage rack 143. The lifting assembly 142 enables multi-layer storage of petri dishes, improving the space utilization of the petri dish storage rack 143.

[0041] Please see Figure 1 and Figure 7 Specifically, the test tube rack 8 is equipped with a test tube oscillation mechanism 15 at its bottom. The oscillation mechanism 15 includes a mounting plate 151 mounted on the bottom surface of the workbench 1 and an oscillation motor 152 vertically mounted on the bottom surface of the mounting plate 151. The output end of the oscillation motor 152 is connected to an eccentric shaft, which is rotatably connected to the test tube rack 8. The oscillation motor 152 drives the eccentric shaft to rotate, achieving periodic oscillation of the test tube rack 8. After the test tube transport mechanism 4 places all the sputum sample test tubes containing digestive fluid back into the test tube rack 8, the oscillation motor 152 starts, driving the eccentric shaft to rotate, causing the test tube rack 8 to vibrate regularly. This vibration is transmitted to the test tubes, ensuring thorough mixing of the sputum and digestive fluid within. Once the mixture is uniform, the oscillation motor 152 stops rotating, and the test tube rack 8 returns to a stationary state. Compared to manual shaking, this method provides more uniform and efficient mixing, ensuring consistent mixing results for each sample, avoiding detection errors due to uneven mixing, and improving the reliability of sample detection results.

[0042] Please see Figure 1 and Figure 2Specifically, a pipette holder 16 is provided on the side adjacent to the digestive fluid storage cup 9, and a plurality of pipettes that cooperate with the pipette pump mechanism 5 are provided on the pipette holder 16.

[0043] Specifically, an infrared sterilizer 17 is provided between the petri dish streaking mechanism 6 and the label printing and pasting mechanism 13.

[0044] Please see Figure 1 and Figure 6 In this embodiment, both the bottom of the petri dish storage rack 143 and the petri dish placement rack are provided with a turntable mechanism that drives the petri dish storage rack 143 and the petri dish placement rack to rotate. The turntable mechanism is existing technology and will not be described in detail here.

[0045] Please see Figure 1 and Figure 2 In this embodiment, the label printing and pasting mechanism 13 includes a label printing component and a label adsorption component arranged adjacent to each other. The label printing component horizontally pushes out the label, which is then adsorbed by the label adsorption component and attached to the bottom surface of the culture dish held by the culture dish transport mechanism 7. The label printing and pasting mechanism 13 is prior art and will not be described in detail here.

[0046] The working process of this utility model is as follows: The testing procedure varies depending on the sample being tested. When testing a sputum sample, the test tube transport mechanism 4 picks up the test tube containing the sputum sample from the test tube rack 8. The test tube transport mechanism 4 moves the test tube along the first frame 2 to the test tube clamping mechanism 10. The test tube clamping mechanism 10 clamps the test tube and weighs it. The test tube transport mechanism 4 clamps the test tube cap and moves it upwards, completing the opening of the test tube. The test tube clamping mechanism 10 moves the opened test tube towards the digestive fluid storage cup 9. The pipette pump mechanism 5 starts, and based on the weighing data from the test tube clamping mechanism 10, a certain amount of digestive fluid is drawn from the digestive fluid storage cup 9 through the pipette and dripped into the test tube, completing the dilution of the sputum sample. The test tube clamping mechanism 10 moves the test tubes back to their original positions, and the test tube transport mechanism 4 picks up the test tubes and puts them back into the test tube rack 8. This process is repeated until all sputum sample test tubes on the test tube rack 8 have been diluted with digestive fluid. At this point, the test tube shaking mechanism 15 is activated, causing the test tube rack 8 to vibrate, thus mixing the sputum and digestive fluid in all the test sample test tubes. Then, the test tube transport mechanism 4 picks up the mixed test tubes and moves them to the test tube clamping mechanism 10. The test tube clamping mechanism 10 holds the test tubes, the barcode scanner 12 automatically scans the test tubes, the test tube transport mechanism 4 opens the test tube caps, and the test tube clamping mechanism 10 moves the opened test tubes. Simultaneously, the petri dish ejection mechanism 11 ejects a single petri dish, and the petri dish transport mechanism 7 moves above the petri dish to open the lid of the untreated petri dish (at this time, the petri dish streaking mechanism 6 moves towards the pipette pump mechanism 5 to avoid it). After opening the lid, the petri dish transport mechanism 7 returns to its position, and the inoculation loop 63 of the petri dish streaking mechanism 6 dips into the sample in the test tube and moves above the opened petri dish for streaking. After streaking is completed, the infrared sterilizer 17 sterilizes the inoculation loop 63, and the petri dish transport mechanism 7 moves to the petri dish ejection mechanism 11 to pick up the streaked petri dish and place it on the label printing and pasting mechanism 13 so that the label is pasted on the bottom of the petri dish. The petri dish transport mechanism 7 then puts it into the petri dish storage mechanism 14.

[0047] When a urine sample needs to be tested, the test tube transport mechanism 4 picks up the test tube and moves it to the test tube clamping mechanism 10. The test tube clamping mechanism 10 holds the test tube, and the test tube transport mechanism 4 opens the cap of the test tube. The test tube clamping mechanism 10 then moves the opened test tube. Simultaneously, the culture dish ejection mechanism 11 ejects a single culture dish, and the culture dish transport mechanism 7 moves above the culture dish to open the cap of the untreated culture dish (at this time, the culture dish streaking mechanism 6 moves towards the pipette pump mechanism 5 to avoid it). After opening the cap, the culture dish transport mechanism 7 returns to its original position, and the inoculation loop 63 of the culture dish streaking mechanism 6 dips into the sample in the test tube and moves it above the opened culture dish for streaking. After streaking is completed, the infrared sterilizer 17 sterilizes the inoculation loop 63. The culture dish transport mechanism 7 moves to the culture dish ejection mechanism 11, picks up the streaked culture dish, and places it on the label printing and pasting mechanism 13 to attach the label to the bottom of the culture dish. The culture dish transport mechanism 7 then places it into the culture dish storage mechanism 14.

[0048] In this invention, the device can detect urine and sputum samples separately. Through the cooperation of the test tube clamping mechanism 10 and other mechanisms, an integrated process of direct urine detection and pre-diluted sputum detection is achieved, simplifying the overall structure of the device, reducing manufacturing costs, and improving the functional integration and detection efficiency. The first frame 2 and the second frame 3 are perpendicularly distributed to each other, and the various mechanisms are arranged along the length of the second frame 3, resulting in a compact layout that makes full use of the surface space of the workbench 1, optimizes the working paths of the various mechanisms on the second frame 3, improves sample detection efficiency, and reduces the equipment footprint. The device has a high degree of automation. From the handling, opening, digestion fluid aspiration, addition, and shaking of test tubes, to the opening, streaking, handling, labeling, and storage of petri dishes, the entire process is automated, reducing manual labor intensity and operational errors, ensuring consistency in sample processing, and improving the accuracy and reliability of test results.

[0049] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application, and should all be included within the protection scope of this application.

Claims

1. A device for digesting, diluting, and streaking microbial samples, comprising a workbench; characterized in that: The workbench is provided with a first frame and a second frame that are perpendicular to each other. A test tube transport mechanism is slidably mounted on the first frame, and a pipetting pump mechanism, a petri dish streaking mechanism, and a petri dish transport mechanism are slidably mounted on the second frame in sequence. A test tube rack is provided adjacent to the first frame, and a digestive fluid storage cup is provided below the pipetting pump mechanism. A test tube clamping mechanism that is slidably connected to the workbench is provided between the test tube rack and the digestive fluid storage cup. The test tube clamping mechanism cooperates with the test tube transport mechanism and the pipetting pump mechanism, respectively. A petri dish ejection mechanism is provided adjacent to the test tube rack, and the petri dish ejection mechanism cooperates with the petri dish streaking mechanism and the petri dish transport mechanism, respectively.

2. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: A barcode scanner is provided between the test tube rack and the test tube clamping mechanism; the test tube clamping mechanism includes a linear displacement component and a clamping component disposed on the worktable, the clamping component includes a clamping base plate connected to the moving end of the linear displacement component, a clamping bracket disposed on the surface of the clamping base plate and a weighing platform, the clamping bracket is provided with a clamping device that cooperates with the weighing platform to clamp the test tubes, and the weighing platform is provided with a weight sensor for weighing the test tubes.

3. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: The adjacent side of the petri dish ejection mechanism along the length of the second frame is provided with a label printing and pasting mechanism and a petri dish storage mechanism for storing processed petri dishes. The petri dish transport mechanism cooperates with the label printing and pasting mechanism and the petri dish storage mechanism to label and store the bottom of the transported petri dishes.

4. The microbial sample digestion, dilution, and inoculation streak device as described in claim 3, characterized in that: The petri dish storage mechanism includes a conveying component, a lifting component, and a petri dish storage rack for placing processed petri dishes on the worktable. The conveying component includes a conveying platform that cooperates with the petri dish handling mechanism to place petri dishes and a conveying drive unit disposed on the conveying platform. The surface of the conveying platform is provided with a conveying chute, and the end of the conveying chute is provided with a circular groove. The output end of the conveying drive unit is provided with a conveying push plate that cooperates with the conveying chute. The conveying push plate pushes the petri dish along the surface of the conveying platform to the circular groove. The lifting component includes a lifting drive unit disposed below the petri dish storage rack. The output end of the lifting drive unit is provided with a circular tray. The circular tray cooperates with the circular groove to push the petri dish upward into the petri dish storage rack.

5. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: The test tube transport mechanism includes an X-axis displacement component slidably connected to the first frame and a fourth Z-axis displacement component disposed on the moving end of the X-axis displacement component. The moving end of the fourth Z-axis displacement component is provided with a gripper. The gripper cooperates with the test tube placement rack to grip the test tube and cooperates with the test tube clamping mechanism to open the cap of the gripped test tube. A first displacement drive component is provided on the first frame, and the output end of the first displacement drive component is connected to the X-axis displacement component.

6. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: The pipetting pump mechanism includes a first Y-axis displacement assembly slidably connected to the second frame and a first Z-axis displacement assembly disposed on the moving end of the first Y-axis displacement assembly, wherein the moving end of the first Z-axis displacement assembly is provided with a pipetting pump. The petri dish streaking mechanism includes a second Y-axis displacement component slidably connected to the second frame and a second Z-axis displacement component disposed on the moving end of the second Y-axis displacement component. The moving end of the second Z-axis displacement component is provided with an inoculation ring. The inoculation ring cooperates with the test tube on the test tube clamping mechanism to streak the petri dish on the petri dish ejection mechanism. The petri dish transport mechanism includes a connecting bracket slidably connected to the second frame and a third Z-axis displacement assembly disposed at the end of the connecting bracket. The moving end of the third Z-axis displacement assembly is provided with a gripper for transporting the petri dish. The second frame is provided with a second displacement drive component, and the output end of the second displacement drive component is connected to the first Y-axis displacement component, the second Y-axis displacement component and the connecting bracket respectively.

7. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: The petri dish ejection mechanism includes a lifting component, a pushing and displacing component, and a petri dish rack for placing untreated petri dishes on the worktable. The lifting component is located below the petri dish rack. The moving end of the pushing and displacing component is provided with a pushing plate. A pushing platform is provided above the pushing and displacing component. The pushing platform is provided with a fixing component and a pushing groove that cooperates with the pushing plate. The lifting component and the fixing component cooperate to fix part of the petri dishes. The pushing plate pushes the lowest petri dish of the petri dish rack to slide along the pushing platform to the end of the pushing groove. A storage slot is provided at the end of the pushing groove. A rotating component that drives the petri dishes to rotate is provided below the storage slot. The petri dish marking mechanism cooperates with the storage slot to mark the petri dishes.

8. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: The test tube rack is equipped with a test tube oscillation mechanism at its bottom. The test tube oscillation mechanism includes a mounting plate disposed on the bottom surface of the workbench and an oscillation motor disposed vertically on the bottom surface of the mounting plate. The output end of the oscillation motor is connected to an eccentric shaft, and the eccentric shaft is rotatably connected to the test tube rack.

9. The microbial sample digestion, dilution, and inoculation streak device as described in claim 1, characterized in that: A pipette holder is provided on the side adjacent to the digestive fluid storage cup, and the pipette holder is provided with a plurality of pipettes that cooperate with the pipette pump mechanism.

10. The microbial sample digestion, dilution, and inoculation streak device as described in claim 3, characterized in that: An infrared sterilizer is provided between the petri dish streaking mechanism and the label printing and pasting mechanism.

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