Dry-type sugar spectrum electrophoresis apparatus

Abstract

The utility model provides a dry type sugar spectrum electrophoresis apparatus. The dry-type sugar spectrum electrophoresis apparatus comprises an outer box body with a cavity inside; the refrigerating device is used for controlling the temperature of the cavity; an imaging device; a data analysis device; an electrophoresis tank is arranged on one side, facing the camera device, of the electrophoresis tank; the composite prefabricated rubber plate is clamped in the electrophoresis tank and comprises a polyacrylamide gel plate, a first agarose gel plate and a second agarose gel plate, the first agarose gel plate and the second agarose gel plate are arranged on the two opposite sides of the polyacrylamide gel plate, and a plurality of sample loading holes are formed in the polyacrylamide gel plate; an ultraviolet lamp and an operation table. According to the electrophoresis device, the temperature can be controlled so as to avoid the influence of Joule heat on a strip result in electrophoresis, real-time visual automatic detection, comparison and semi-quantitative analysis can be realized, and the problems of large dosage of an electrophoresis buffer solution required in wet electrophoresis and complicated operation can be effectively avoided by adopting a dry electrophoresis apparatus.

Description

Technical Field

[0001] This utility model relates to a laboratory testing device, and more particularly to a dry glycosylation electrophoresis apparatus. Background Technology

[0002] Glycosyl profile electrophoresis is a commonly used analytical method for glycan chain structure analysis and quality control of polysaccharides and proteoglycans in traditional Chinese medicine. However, current glycosyl profile electrophoresis instruments all have certain drawbacks.

[0003] For example, patent application number 202220475429.6, entitled "Glycosylation Electrophoresis Apparatus," discloses a detection system for analyzing carbohydrate components. However, it uses polyacrylamide gel electrophoresis (PAGE) vertical electrophoresis, requiring a large volume of electrophoresis buffer, resulting in a complex system structure and difficulty in achieving multi-channel sample loading and automation. While dry electrophoresis can overcome the problem of large buffer volume, currently disclosed dry electrophoresis methods also have some shortcomings. For instance, some dry electrophoresis devices have a relatively primitive structure without matching cooling devices, leading to Joule heating during electrophoresis, causing band dispersion and affecting separation efficiency and resolution. Furthermore, they lack data analysis capabilities, requiring the acquired band images to be combined with other analytical software for analysis, which is time-consuming and labor-intensive. Additionally, multi-sample analysis is not possible; the time-consuming multi-sample loading leads to poor consistency in the electrophoresis process, ultimately affecting the accuracy of the analytical results. The gel plates required for dry electrophoresis are prepared by pre-filling agarose (containing EB) between two layers of transparent acrylic plates and sealing the perimeter of the acrylic plates with paraffin. However, this type of gel plate has the problem of not being able to achieve sugar separation. Utility Model Content

[0004] Based on the above problems, the purpose of this utility model is to provide a dry glycosylation electrophoresis apparatus. This electrophoresis device can control the temperature to avoid the influence of Joule heat on the band results during electrophoresis. It can realize real-time visual automatic detection, comparison and semi-quantitative analysis. Using a dry electrophoresis apparatus can effectively avoid the problems of large amount of electrophoresis buffer required and complicated operation in wet electrophoresis.

[0005] To achieve the above objectives, this utility model provides a dry glycosylation electrophoresis apparatus, comprising:

[0006] An outer casing with a cavity inside, and an opening communicating with the cavity and a cover sealing the opening on one side of the outer casing;

[0007] A refrigeration device, which is fixed to the outer casing and controls the temperature of the cavity;

[0008] A camera device is located on the upper part of the outer casing, and the camera area at least covers the cavity;

[0009] A data analysis device that converts electrophoretic bands in the image obtained by the camera device into chromatographic peaks based on their gray intensity and analyzes the peak areas.

[0010] An electrophoresis pool is housed in the cavity through the opening. An electrophoresis tank is provided on the side of the electrophoresis pool facing the camera device, and a positive electrode interface and a negative electrode interface are respectively provided on opposite sides of the electrophoresis tank.

[0011] A composite pre-fabricated gel plate is inserted into the electrophoresis tank. The composite pre-fabricated gel plate includes a polyacrylamide gel plate and a first agarose gel plate and a second agarose gel plate located on opposite sides of the polyacrylamide gel plate. The first agarose gel plate and the second agarose gel plate are respectively connected to the positive electrode interface and the negative electrode interface. The polyacrylamide gel plate is provided with a plurality of sample loading wells.

[0012] An ultraviolet lamp is located on the upper part of the outer casing, and the ultraviolet radiation area at least covers the cavity.

[0013] The control panel is fixed to the outer casing and is equipped with a control system that controls the operation of the refrigeration device, the camera device, the data analysis device, the electric swimming pool and the ultraviolet lamp respectively.

[0014] Compared with the prior art, the dry glycosylation electrophoresis apparatus of this invention has at least the following technical effects.

[0015] (1) The electrophoresis pool is housed in a cavity through an opening, and the opening is sealed by a cover, thus keeping the electrophoresis pool in a closed cavity. In addition, a refrigeration device is fixed to the outer casing. The refrigeration device absorbs the Joule heat generated during the electrophoresis process, thus keeping the electrophoresis pool at a low temperature and avoiding the influence of the generated Joule heat on the strip results.

[0016] (2) Electrophoresis is performed using a composite precast gel plate composed of a first agarose gel plate, a polyacrylamide gel plate, and a second agarose gel plate. As a dry electrophoresis method, this effectively avoids the problems of large amounts of electrophoresis buffer and complex operations required in wet electrophoresis. Furthermore, the first and second agarose gel plates are connected to the positive and negative electrodes, respectively, and the polyacrylamide gel in the middle serves as the electrophoresis separation medium, achieving good sugar separation. Connecting the first and second agarose gel plates to the positive and negative electrodes respectively solves the problem of difficulty in connecting electrodes to the polyacrylamide gel plate. The composite precast gel plate is inserted into the electrophoresis tank of the electrophoresis pool. The polyacrylamide gel plate has several sample loading holes for easy sample loading.

[0017] (3) The ultraviolet lamp is located at the top of the outer casing, and the ultraviolet radiation area at least covers the cavity to meet the fluorescence environment required for electrophoresis. The camera device is located at the top of the outer casing, and the camera area at least covers the cavity to acquire the electrophoresis process results in real time. The data analysis device converts the electrophoretic bands in the image obtained by the camera device into chromatographic peaks according to their gray intensity and analyzes the peak area, which can realize automatic detection, comparison and semi-quantitative analysis of gel bands.

[0018] As a technical solution of this utility model, the electric swimming pool and the cavity are connected in a drawer-like manner, and the opening and the cover are connected in an integral or separate manner.

[0019] As a technical solution of this utility model, the refrigeration device includes a cooling pipe, through which coolant flows, and the cooling pipe is arranged around the inner peripheral wall of the cavity.

[0020] As a technical solution of this utility model, the cavity is recessed to form an annular groove, and the cooling pipe is snapped into the annular groove.

[0021] As a technical solution of this utility model, the electric pool is provided with a sample tank at one end near the opening, and the sample tank is detachably connected to the electric pool.

[0022] As a technical solution of this utility model, the side of the electric pool facing the camera device has a recessed groove formed at the end near the opening, and the sample tank overlaps in the groove.

[0023] As a technical solution of this utility model, the sample tank includes multiple independent liquid storage chambers.

[0024] As a technical solution of this utility model, the ultraviolet lamp includes two, and the camera device is located in the middle of the two ultraviolet lamps.

[0025] As a technical solution of this utility model, the control system includes a refrigeration control system for controlling the operation of the refrigeration device, an electrophoresis control system for controlling the operation of the electrophoresis pool, a data analysis control system for controlling the operation of the data analysis device, a camera control system for controlling the operation of the camera device, and an ultraviolet lamp control system for controlling the operation of the ultraviolet lamp.

[0026] As a technical solution of this utility model, one side of the outer casing is provided with a power switch electrically connected to the electrophoresis control system, an ultraviolet lamp switch electrically connected to the ultraviolet lamp control system, and a refrigeration device switch electrically connected to the refrigeration control system. Attached Figure Description

[0027] Figure 1 This is a three-dimensional perspective view of the dry glycosylation electrophoresis apparatus of this utility model.

[0028] Figure 2 This is a three-dimensional perspective view of the dry glycosylation electrophoresis instrument of this utility model before sample testing.

[0029] Figure 3 This is a front view of the composite precast gel plate of the dry glycosylation electrophoresis apparatus of this utility model.

[0030] Figure 4 This is the electrophoretic pattern of a polysaccharide sample in the dry glycospectroscopy electrophoresis apparatus of this invention.

[0031] Figure 5 The results are the result of data analysis and processing of the dry glycosylation electrophoresis instrument of this utility model.

[0032] Component Symbol Explanation

[0033] 100-Dry agarose gel electrophoresis apparatus; 10-Outer casing; 11-Cavity; 13-Opening; 15-Lid; 20-Refrigeration device; 21-Cooling pipe; 30-Camera device; 40-Electrophoresis pool; 41-Electrophoresis tank; 43-Positive electrode interface; 45-Negative electrode interface; 47-Sample tank; 471-Reservoir chamber; 50-Composite precast gel plate; 51-First agarose gel plate; 53-Polyacrylamide gel plate; 55-Second agarose gel plate; 57-Sample loading well; 60-UV lamp; 61-First UV lamp; 63-Second UV lamp; 70-Operating table; 71-Display screen; 73-Power switch; 75-UV lamp switch; 77-Refrigeration device switch; S1-First large surface; S2-First side surface; S3-Second side surface; S4-Third side surface; S5-Fourth side surface; S6-Second large surface Detailed Implementation

[0034] To better illustrate the purpose, technical solution, and beneficial effects of this utility model, the following description, in conjunction with specific accompanying drawings, will provide further details. It should be noted that the embodiments described below are further explanations of this utility model and should not be construed as limiting the scope of protection of this utility model.

[0035] like Figures 1-2 As shown, the dry glycosylation electrophoresis apparatus 100 includes an outer casing 10, a cooling device 20, a camera device 30, a data analysis device, an electrophoresis pool 40, a composite precast gel plate 50, an ultraviolet lamp 60, and an operating table 70.

[0036] The outer casing 10 includes a first large surface S1 and a second large surface S6, and a first side surface S2, a second side surface S3, a third side surface S4, and a fourth side surface S5 connected sequentially between the first large surface S1 and the second large surface S6. The first large surface S1 serves as the top of the outer casing 10, and a camera device 30 and an ultraviolet lamp 60 can be installed on it. The outer casing 10 has a cavity 11, and an opening 13 communicating with the cavity 11 and a cover 15 sealing the opening 13 are provided on the first side surface S2. The opening 13 and the cover 15 are connected integrally or separately. The cover 15 can be pivotally connected to the opening 13, so that the opening 13 can be opened to place the electrolytic cell 40 when measuring or changing samples, and then closed to form a sealed space. Of course, the cover 15 can also be separate from the opening 13, with the electrolytic cell 40 placed in the cavity 11 and the opening 13 sealed by the cover 15.

[0037] The operating table 70 is fixed to the outer casing 10. The operating table 70 is equipped with a control system (not shown) that controls the operation of the cooling device 20, the camera device 30, the data analysis device (not shown in the figure), the electrophoresis pool 40, and the ultraviolet lamp 60. Further, the control system includes a cooling control system for controlling the operation of the cooling device 20, an electrophoresis control system for controlling the operation of the electrophoresis pool 40, a data analysis control system for controlling the operation of the data analysis device, a camera control system for controlling the operation of the camera device 30, and an ultraviolet lamp control system for controlling the operation of the ultraviolet lamp 60. The cooling control system can adjust the temperature. The electrophoresis control system can control the power supply and adjust the high and low voltage. The data analysis control system can convert the electrophoretic bands in the image into chromatographic peaks based on their grayscale intensity, and obtain the analysis results of carbohydrate components by comparing the peak areas. The camera control system can control the taking of pictures and adjust the camera focus and anti-fog lamp effect, thereby achieving real-time and efficient video recording. The ultraviolet lamp control system can adjust the ultraviolet lamp wavelength to facilitate the selection of different wavelengths. The refrigeration control system, electrophoresis control system, data analysis control system, camera control system, and ultraviolet lamp control system can use conventional system hardware and software, requiring only the implementation of their respective functions. A display screen 71 can be installed on the control panel 70, where corresponding parameters are set to issue commands to each system. The display screen 71 can also display the electrophoresis process and data analysis results in real time. The control panel 70 can be located in the areas corresponding to the first large surface S1, the second side S3, and the third side S4 of the outer casing 10. Furthermore, one side of the outer casing 10, such as the second side S3, is equipped with a power switch 73 electrically connected to the electrophoresis control system, an ultraviolet lamp on / off switch 75 electrically connected to the ultraviolet lamp control system, and a refrigeration device switch 77 electrically connected to the refrigeration control system.

[0038] The refrigeration unit 20 is fixed to the outer casing 10 and controls the temperature of the cavity 11. The refrigeration unit 20 includes a cooling pipe 21 through which coolant flows, and the cooling pipe 21 is arranged around the inner peripheral wall of the cavity 11. Further, an annular groove (not shown in the figure) is formed within the cavity 11, and the cooling pipe 21 is engaged in the annular groove. The cooling pipe 21 may include a coolant inlet and a coolant outlet. Coolant enters through the coolant inlet and exits through the coolant outlet. Of course, a valve may be provided at the coolant outlet to allow coolant to circulate within the cooling pipe 20, and to discharge coolant only when the coolant temperature is too high, allowing new coolant to enter through the coolant inlet.

[0039] This method not only makes the structure compact but also maintains a uniform temperature distribution within cavity 11. Temperature regulation can be achieved by controlling the flow rate of the coolant through parameter settings in the refrigeration control system.

[0040] The camera device 30 is located on the first large surface S1 of the upper part of the outer casing 11, and the imaging area at least covers the cavity 11. Similarly, the ultraviolet lamp 60 is also located on the first large surface S1 of the upper part of the outer casing 11, and the ultraviolet light radiating area of ​​the ultraviolet lamp 60 at least covers the cavity 11. Two ultraviolet lamps 60 may be included, such as a first ultraviolet lamp 61 and a second ultraviolet lamp 63. The camera device 30 is located precisely between the two ultraviolet lamps 60, resulting in a compact structure and maintaining a uniform fluorescence distribution within the cavity 11. A memory card (not shown in the figure) may be installed inside the camera device 30, facilitating timely recording and storage of the analysis process and results.

[0041] The electrophoresis pool 40 is housed within the cavity 11 via an opening 13, and the electrophoresis pool 40 and the cavity 11 are connected in a drawer-like manner. A slide rail (not shown in the figure) may be provided on the inner wall of the cavity 11, and a slider (not shown in the figure) may be provided on the outer wall of the electrophoresis pool 40. The electrophoresis pool 40 and the cavity 11 can be connected in a drawer-like manner through the action of the slide rail and the slider. Of course, other structures can also be used to connect the electrophoresis pool 40 and the cavity 11, as long as they satisfy the drawer-like connection requirement. An electrophoresis tank 41 is provided on the side of the electrophoresis pool 40 facing the camera device 20, and a positive electrode interface 43 and a negative electrode interface 45 are respectively provided on opposite sides of the electrophoresis tank 41. After the positive terminal 43 and negative terminal 45 are housed in the cavity 11 of the electrophoresis pool 40, they can be electrically connected to the power switch 73 and the electrophoresis control system. Furthermore, wires and interface terminals can be embedded in the cavity 11, and the positive terminal 43 and negative terminal 45 can be inserted into the interface terminals after being housed in the cavity 11 of the electrophoresis pool 40 to achieve electrical connection. Of course, in actual operation, other methods can also be used, as long as the positive terminal 43 and negative terminal 45 can be electrically connected to the power switch 73 and the electrophoresis control system after being housed in the cavity 11 of the electrophoresis pool 40. The electrophoresis pool 40 has a sample tank 47 at one end near the opening 13. The sample tank 47 is detachably connected to the electrophoresis pool 40, allowing for portable sample changing. On the side of the electrophoresis pool 40 facing the camera device 30, a recessed groove (not shown in the figure) is formed at the end near the opening 13, and the sample tank 47 overlaps in the groove (not shown in the figure). The sample tank 47 includes multiple independent liquid storage chambers 471. The arrangement of multiple independent liquid storage chambers 471 in the sample tank 47 can avoid the time-consuming sample loading when performing multi-sample analysis, thereby maintaining the consistency of the electrophoresis process and ensuring the accuracy of the analysis results.

[0042] This utility model is a dry electrophoresis apparatus, which uses a composite prefabricated gel plate 50. For example... Figure 3 As shown, the composite pre-fabricated gel plate 50 is mounted in the electrophoresis tank 41. The composite pre-fabricated gel plate 50 includes a polyacrylamide gel plate 53 and a first agarose gel plate 51 and a second agarose gel plate 55 located on opposite sides of the polyacrylamide gel plate 53. The first agarose gel plate 51 and the second agarose gel plate 55 are respectively connected to the positive electrode interface 43 and the negative electrode interface 45. The polyacrylamide gel plate 53 is provided with a plurality of sample loading holes 57. The sample in the liquid storage chamber 471 of the sample tank 47 can be placed into the polyacrylamide gel plate 53 through the sample loading holes 57 and separated in the polyacrylamide gel plate 53 under the action of electrophoresis.

[0043] The dry glycospectroscopy electrophoresis apparatus 100 of this invention, through its structural design including an outer casing 10, a cooling device 20, a camera device 30, a data analysis device, an electrophoresis pool 40, a composite precast gel plate 50, an ultraviolet lamp 60, and an operating table 70, allows for temperature control to avoid the influence of Joule heating on the band results during electrophoresis. It enables real-time, visual, automatic detection, comparison, and semi-quantitative analysis, effectively avoiding the problems of large amounts of electrophoresis buffer and complex operations required in wet electrophoresis. For example, the dry glycospectroscopy electrophoresis apparatus 100 of this invention was used to analyze two polysaccharide samples, and the results are as follows: Figure 4 As shown, using the dry glycospectroscopy electrophoresis apparatus of this invention, complete electrophoretic patterns can be obtained for 100 pairs of two polysaccharide samples. Further data analysis is then performed... Figure 5 As can be seen, corresponding chromatographic peaks can be obtained for each.

[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model and are not intended to limit the scope of protection of this utility model. Although this utility model has been described in detail with reference to preferred embodiments, it is not limited to those listed in the embodiments. Those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the essence and scope of the technical solutions of this utility model.

Claims

1. A dry glycosylation electrophoresis apparatus, characterized in that, include: An outer casing with a cavity inside, and an opening communicating with the cavity and a cover sealing the opening on one side of the outer casing; A refrigeration device, which is fixed to the outer casing and controls the temperature of the cavity; A camera device is located on the upper part of the outer casing, and the camera area at least covers the cavity; A data analysis device that converts electrophoretic bands in the image obtained by the camera device into chromatographic peaks based on their gray intensity and analyzes the peak areas. An electrophoresis pool is housed in the cavity through the opening. An electrophoresis tank is provided on the side of the electrophoresis pool facing the camera device, and a positive electrode interface and a negative electrode interface are respectively provided on opposite sides of the electrophoresis tank. A composite pre-fabricated gel plate is inserted into the electrophoresis tank. The composite pre-fabricated gel plate includes a polyacrylamide gel plate and a first agarose gel plate and a second agarose gel plate located on opposite sides of the polyacrylamide gel plate. The first agarose gel plate and the second agarose gel plate are respectively connected to the positive electrode interface and the negative electrode interface. The polyacrylamide gel plate is provided with a plurality of sample loading wells. An ultraviolet lamp is located on the upper part of the outer casing, and the ultraviolet radiation area at least covers the cavity. The control panel is fixed to the outer casing and is equipped with a control system that controls the operation of the refrigeration device, the camera device, the data analysis device, the electric swimming pool and the ultraviolet lamp respectively.

2. The dry glycosylation electrophoresis apparatus according to claim 1, characterized in that, The electric pool and the cavity are connected in a drawer-like manner, and the opening and the cover are connected in an integral or separate manner.

3. The dry glycosylation electrophoresis apparatus according to claim 1, characterized in that, The refrigeration device includes a cooling pipe through which coolant flows, and the cooling pipe is arranged around the inner peripheral wall of the cavity.

4. The dry glycosylation electrophoresis apparatus according to claim 3, characterized in that, The cavity is recessed to form an annular groove, and the cooling pipe is engaged in the annular groove.

5. The dry glycosylation electrophoresis apparatus according to claim 1, characterized in that, The electric pool has a sample tank at one end near the opening, and the sample tank is detachably connected to the electric pool.

6. The dry glycosylation electrophoresis apparatus according to claim 5, characterized in that, The side of the electric pool facing the camera device has a recessed groove at the end near the opening, and the sample tank overlaps the groove.

7. The dry glycosylation electrophoresis apparatus according to claim 6, characterized in that, The sample tank includes multiple independent liquid storage chambers.

8. The dry glycosylation electrophoresis apparatus according to claim 1, characterized in that, The ultraviolet lamps include two, and the camera device is located in the middle of the two ultraviolet lamps.

9. The dry glycosylation electrophoresis apparatus according to claim 1, characterized in that, The control system includes a refrigeration control system for controlling the operation of the refrigeration device, an electrophoresis control system for controlling the operation of the electrophoresis pool, a data analysis control system for controlling the operation of the data analysis device, a camera control system for controlling the operation of the camera device, and an ultraviolet lamp control system for controlling the operation of the ultraviolet lamp.

10. The dry glycosylation electrophoresis apparatus according to claim 9, characterized in that, One side of the outer casing is equipped with a power switch electrically connected to the electrophoresis control system, an ultraviolet lamp switch electrically connected to the ultraviolet lamp control system, and a refrigeration device switch electrically connected to the refrigeration control system.

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