A contamination-resistant centrifuge tube device for micro-DNA extraction

Abstract

This invention discloses a contamination-proof centrifuge tube device for micro-DNA extraction, relating to the field of DNA extraction technology. The device includes a centrifuge tube body with a first threaded groove on its inner wall. A connecting mechanism is installed inside the centrifuge tube body through the first threaded groove. A third thread is fixedly connected to the outer wall of the centrifuge tube body. A second sealing mechanism is provided at the top of the connecting mechanism. Through the second sealing mechanism, the liquid silicone valve deforms during the insertion or removal of the dropper, effectively preventing contamination. The first sealing mechanism effectively blocks the second sealing mechanism, preventing liquid leakage during centrifugation and preventing bacteria from entering the centrifuge tube body and contaminating the liquid due to deformation of the liquid silicone valve during centrifugation.

Description

Technical Field

[0001] This utility model relates to the field of DNA extraction technology, specifically a contamination-proof centrifuge tube device for micro-DNA extraction. Background Technology

[0002] DNA extraction centrifuge tubes are core consumables in molecular biology experiments. Their core function is to serve as a complete reaction vessel, carrying out sample lysis, nucleic acid separation, and purification operations. During extraction, the device (usually 1.5ml) first contains the biological sample with lysis buffer. High-speed centrifugation (e.g., 12000-15000rpm) separates cell debris and protein impurities from the nucleic acid supernatant. Subsequently, in the purification stage, repeated buffer washing and centrifugation are performed to gradually remove residual impurities and precipitate the target DNA / RNA. Its polypropylene material combines resistance to strong corrosive reagents (such as phenol and chloroform) with high mechanical strength, ensuring centrifugation safety.

[0003] Existing DNA extraction centrifuge tube devices have poor anti-contamination structures during use. When adding liquids and reagents, external bacteria can easily be introduced into the centrifuge tube, causing contamination of the liquid and affecting subsequent DNA extraction. Furthermore, aerosols are easily generated during centrifugation, and DNA fragments carried in the aerosols can easily lead to distorted experimental results.

[0004] Based on this, a contamination-proof centrifuge tube device for micro-DNA extraction is now provided, which can eliminate the drawbacks of existing devices. Utility Model Content

[0005] The purpose of this invention is to provide a contamination-proof centrifuge tube device for micro-DNA extraction, in order to solve the problems in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A contamination-resistant centrifuge tube device for micro-DNA extraction includes a centrifuge tube body, a first screw groove on the inner wall of the centrifuge tube body, a connecting mechanism installed inside the centrifuge tube body through the first screw groove, and a third thread fixedly connected to the outer wall of the centrifuge tube body.

[0008] The top of the connecting mechanism is provided with a second sealing mechanism, and the top of the centrifuge tube body is installed with a first sealing mechanism via a third thread.

[0009] Based on the above technical solutions, this utility model also provides the following optional technical solutions:

[0010] In one alternative: the connecting mechanism includes a connector, the connector is externally fixedly connected with a first thread, and the first thread is adapted to a first threaded groove, and the inner wall of the connector is provided with a second threaded groove.

[0011] In one alternative: a filter mechanism is provided at the bottom of the connector.

[0012] In one alternative: the filtration mechanism includes a filter chamber, the outer wall of which is fixedly connected with a second thread, and the second thread is adapted to a second thread groove.

[0013] In one alternative: the second sealing mechanism includes a sealing cap, which is fixedly connected to the top of the connector, and a liquid silicone valve is provided inside the sealing cap, and an anti-slip groove is provided on the outer wall of the sealing cap.

[0014] In one alternative: the first sealing mechanism includes a top cover, the inner wall of which has a third threaded groove that is adapted to a third thread, and the interior of the top cover has an installation groove, the interior of which is provided with a sealing component.

[0015] In one alternative: the sealing assembly includes a sealing block that is slidably mounted inside a mounting groove, a spring is fixedly connected to the top of the sealing block, and the top of the spring is fixedly connected to a top cover, and the sealing block is adapted to a liquid silicone valve.

[0016] In one alternative: a sealing ring is provided between the connector and the centrifuge tube body, and a sealing ring is provided between the centrifuge tube body and the top cover.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0018] 1. The present invention, through the setting of the second sealing mechanism, causes the liquid silicone valve to deform during the insertion or removal of the dropper, effectively preventing contamination. The setting of the first sealing mechanism effectively blocks the second sealing mechanism, preventing liquid from leaking out from the second sealing mechanism during centrifugation. At the same time, it prevents the liquid silicone valve from deforming during centrifugation, thus preventing bacteria from entering the interior of the centrifuge tube and contaminating the liquid.

[0019] 2. This utility model effectively filters liquids through a filter chamber. DNA-containing aerosols generated during centrifugation are intercepted by the 0.22μm pore size filter chamber, blocking their diffusion path. At the same time, it avoids interference from chemical additives on DNA samples. The filter chamber is made of 304 stainless steel. The second thread and second screw groove make it easy to disassemble the filter chamber for cleaning and disinfection and reuse, reducing the cost of use. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0021] Figure 2 This is a schematic diagram of the installation structure of the connection mechanism of this utility model.

[0022] Figure 3 This is a schematic diagram of the filter mechanism of this utility model.

[0023] Figure 4 This is a schematic diagram of the first sealing mechanism of this utility model.

[0024] Figure reference numerals: 1. Centrifuge tube body; 2. First threaded groove; 3. Connecting mechanism; 31. Connector; 32. First thread; 33. Second threaded groove; 4. First sealing mechanism; 41. Top cover; 42. Third threaded groove; 43. Mounting groove; 44. Sealing block; 45. Spring; 5. Second sealing mechanism; 51. Sealing cap; 52. Liquid silicone valve; 53. Anti-slip groove; 6. Filtration mechanism; 61. Filter chamber; 62. Second thread; 7. Third thread. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments.

[0026] In one embodiment, such as Figures 1-4 As shown, a contamination-proof centrifuge tube device for micro DNA extraction includes a centrifuge tube body 1, a first screw groove 2 is provided on the inner wall of the centrifuge tube body 1, a connecting mechanism 3 is installed inside the centrifuge tube body 1 through the first screw groove 2, and a third thread 7 is fixedly connected to the outer wall of the centrifuge tube body 1.

[0027] The top of the connecting mechanism 3 is provided with a second sealing mechanism 5, and the top of the centrifuge tube body 1 is installed with a first sealing mechanism 4 through a third thread 7.

[0028] In this embodiment, the filter mechanism 6 is assembled with the connecting mechanism 3, and then the connecting mechanism 3 is assembled with the centrifuge tube body 1. The liquid from which DNA needs to be extracted is drawn using a dropper. The dropper is inserted into the connecting mechanism 3 through the second sealing mechanism 5. The dropper is squeezed to allow the liquid to enter the centrifuge tube body 1. Then, the first sealing mechanism 4 is installed with the centrifuge tube body 1. Finally, the centrifuge tube is placed in the centrifuge. The centrifuge drives the centrifuge tube to rotate, effectively completing the centrifugation separation. Due to the setting of the second sealing mechanism 5, the liquid silicone valve 52 will deform during the insertion or removal of the dropper, effectively preventing contamination. Due to the setting of the first sealing mechanism 4, the second sealing mechanism 5 is effectively blocked, preventing liquid from leaking out from the second sealing mechanism 5 during centrifugation. At the same time, it prevents the liquid silicone valve 52 from deforming during centrifugation, which could allow bacteria to enter the centrifuge tube body 1 and contaminate the liquid.

[0029] In one embodiment, such as Figure 2 and Figure 3 As shown, the connecting mechanism 3 includes a connector 31, with a first thread 32 fixedly connected to the outside of the connector 31, and the first thread 32 is adapted to the first screw groove 2. A second screw groove 33 is provided on the inner wall of the connector 31. A filtering mechanism 6 is provided at the bottom of the connector 31. The filtering mechanism 6 includes a filter chamber 61, with a second thread 62 fixedly connected to the outer wall of the filter chamber 61, and the second thread 62 is adapted to the second screw groove 33. The filter chamber 61 effectively filters the liquid. DNA-containing aerosols generated during centrifugation are intercepted by the 0.22μm pore size filter chamber 61, blocking their diffusion path and avoiding interference from chemical additives to the DNA sample. The filter chamber 61 is made of 304 stainless steel. The second thread 62 and the second screw groove 33 facilitate the disassembly of the filter chamber 61 for cleaning and disinfection and reuse, reducing the cost of use.

[0030] In one embodiment, such as Figure 2 and Figure 3 As shown, the second sealing mechanism 5 includes a sealing cover 51, which is fixedly connected to the top of the connector 31. A liquid silicone valve 52 is provided inside the sealing cover 51, and an anti-slip groove 53 is provided on the outer wall of the sealing cover 51. When adding liquid or medicine with a dropper, the dropper is kept clean. Each time the dropper is pulled out, the liquid silicone valve 52 deforms and closes, so that external bacteria and other contaminants will not enter the interior of the centrifuge tube body 1, thus playing a role in preventing contamination.

[0031] In one embodiment, such as Figure 2 and Figure 4As shown, the first sealing mechanism 4 includes a top cover 41. The inner wall of the top cover 41 has a third threaded groove 42, which is adapted to the third thread 7. The top cover 41 has an installation groove 43 inside, and a sealing component is provided inside the installation groove 43. The sealing component includes a sealing block 44, which is slidably installed inside the installation groove 43. A spring 45 is fixedly connected to the top of the sealing block 44, and the top of the spring 45 is fixedly connected to the top cover 41. The sealing block 44 is adapted to the liquid silicone valve 52. The top cover 41 is threadedly installed to the centrifuge tube body 1 through the cooperation of the third threaded groove 42 and the third thread 7. During the installation of the top cover 41, the sealing block 44 is inserted into the liquid silicone valve 52, and the spring 45 deforms. The sealing block 44 effectively blocks the liquid silicone valve 52, preventing the liquid silicone valve 52 from deforming during centrifugation, and preventing liquid from leaking out through the liquid silicone valve 52 or bacteria from entering the centrifuge tube body 1 through the liquid silicone valve 52.

[0032] In one embodiment, such as Figure 2 and Figure 3 As shown, a sealing ring is provided between the connector 31 and the centrifuge tube body 1, and a sealing ring is provided between the centrifuge tube body 1 and the top cover 41 to improve the sealing performance, prevent the entry of miscellaneous bacteria, and play a role in preventing contamination.

[0033] The above embodiment discloses a contamination-resistant centrifuge tube device for micro-DNA extraction. The filter chamber 61 and connector 31 are assembled by the cooperation of the second thread 62 and the second screw groove 33. The connector 31 is then installed to the centrifuge tube body 1 by the cooperation of the first screw groove 2 and the first thread 32. A dropper is used to draw the liquid from which DNA needs to be extracted. The dropper is inserted into the connecting mechanism 3 through the second sealing mechanism 5, and the dropper is squeezed to allow the liquid to enter the centrifuge tube body 1. The top cover 41 is then installed to the centrifuge tube body 1 by the cooperation of the third thread 7 and the third screw groove 42. During the installation of the top cover 41, a sealing block 44 is inserted into the liquid silicone valve 52, and the spring 45 deforms. The sealing block 44 effectively blocks the liquid silicone valve 52, preventing deformation of the liquid silicone valve 52 during centrifugation, thus preventing leakage of liquid or bacteria from entering the centrifuge tube body 1 through the liquid silicone valve 52. Finally, the centrifuge tube is placed in a centrifuge, and the centrifuge rotates the centrifuge tube to effectively complete the centrifugation separation.

[0034] When adding liquids or medications using a dropper, ensure the dropper is clean. Each time the dropper is removed, the liquid silicone valve 52 deforms and closes, preventing external bacteria from entering the centrifuge tube body 1 and thus preventing contamination. The liquid is effectively filtered through the filter chamber 61. DNA-containing aerosols generated during centrifugation are intercepted by the 0.22μm pore size filter chamber 61, blocking their diffusion path and preventing interference from chemical additives to the DNA sample. The filter chamber 61 is made of 304 stainless steel. The second thread 62 and the second screw groove 33 make it easy to disassemble the filter chamber 61 for cleaning and disinfection before reuse, reducing operating costs.

[0035] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A contamination-resistant centrifuge tube device for micro DNA extraction, comprising a centrifuge tube body (1), wherein a first screw groove (2) is provided on the inner wall of the centrifuge tube body (1), a connecting mechanism (3) is installed inside the centrifuge tube body (1) through the first screw groove (2), and a third thread (7) is fixedly connected to the outer wall of the centrifuge tube body (1); Its features are, The top of the connecting mechanism (3) is provided with a second sealing mechanism (5), and the top of the centrifuge tube body (1) is provided with a first sealing mechanism (4) through a third thread (7).

2. The anti-contamination centrifuge tube device for micro-DNA extraction according to claim 1, characterized in that, The connecting mechanism (3) includes a connector (31), the connector (31) is fixedly connected to the outside with a first thread (32), and the first thread (32) is adapted to the first thread groove (2), and the inner wall of the connector (31) is provided with a second thread groove (33).

3. The anti-contamination centrifuge tube device for micro-DNA extraction according to claim 2, characterized in that, A filter mechanism (6) is provided at the bottom of the connector (31).

4. The anti-contamination centrifuge tube device for micro-DNA extraction according to claim 3, characterized in that, The filtering mechanism (6) includes a filter chamber (61), the outer wall of which is fixedly connected with a second thread (62), and the second thread (62) is adapted to the second thread groove (33).

5. A contamination-resistant centrifuge tube device for micro-DNA extraction according to claim 2, characterized in that, The second sealing mechanism (5) includes a sealing cover (51), which is fixedly connected to the top of the connector (31). A liquid silicone valve (52) is provided inside the sealing cover (51), and an anti-slip groove (53) is provided on the outer wall of the sealing cover (51).

6. The anti-contamination centrifuge tube device for micro-DNA extraction according to claim 5, characterized in that, The first sealing mechanism (4) includes a top cover (41), the inner wall of the top cover (41) is provided with a third threaded groove (42), and the third threaded groove (42) is adapted to the third thread (7). The top cover (41) is provided with an installation groove (43), and a sealing component is provided inside the installation groove (43).

7. A contamination-resistant centrifuge tube device for micro-DNA extraction according to claim 6, characterized in that, The sealing assembly includes a sealing block (44) which is slidably installed inside the mounting groove (43). A spring (45) is fixedly connected to the top of the sealing block (44), and the top of the spring (45) is fixedly connected to the top cover (41). The sealing block (44) is compatible with the liquid silicone valve (52).

8. A contamination-resistant centrifuge tube device for micro-DNA extraction according to claim 6, characterized in that, A sealing ring is provided between the connector (31) and the centrifuge tube body (1), and a sealing ring is provided between the centrifuge tube body (1) and the top cover (41).

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