Cell imaging equipment and workstation

Abstract

This application discloses a cell imaging device and workstation, relating to the field of biodetection technology, to alleviate the problem of operators accidentally inserting or removing samples during operation of cell imaging devices in related technologies. The cell imaging device includes a frame, a sample mounting device, a light source device, an imaging device, and a shielding component. The sample mounting device is mounted on the frame and includes a sample mounting port; the light source device is mounted on the frame and provides illumination light to the sample on the sample mounting device; the imaging device is mounted on the frame and located to one side of the sample mounting device; the shielding component is movably mounted on the frame; when the shielding component is in a first position, the sample can be mounted onto the sample mounting device through the sample mounting port; when the shielding component is in a second position, the shielding component blocks the sample mounting path corresponding to the sample mounting port. Therefore, this application has the advantages of improving the sample protection and application reliability of the cell imaging device.

Description

Technical Field

[0001] This application relates to the field of biological detection technology, and more specifically, to a cell imaging device and workstation. Background Technology

[0002] Cell imaging equipment is an essential basic instrument in the field of life science research. As a device that can quickly and accurately measure cell number and viability, cell imaging equipment is widely used in biomedical research and drug development.

[0003] Currently available cell imaging equipment often suffers from problems during actual use, such as operators accidentally inserting or removing samples during operation. This can lead to sudden termination of cell imaging, excessive errors in detection results, and even damage to the samples and the cell imaging equipment, affecting experimental progress and accuracy. Consequently, the reliability of cell imaging equipment is not high. Utility Model Content

[0004] The purpose of this application is to provide a cell imaging device and workstation, which improves the problem of malfunction of the cell imaging device and enhances the application reliability of the cell imaging device by setting a movable shielding component.

[0005] The embodiments of this application are implemented as follows:

[0006] A first aspect of this application provides a cell imaging device, including a frame, a sample mounting device, a light source device, an imaging device, and a shielding member. The sample mounting device is mounted on the frame and includes a sample mounting port. The light source device is mounted on the frame and can provide illumination light to the sample on the sample mounting device. The imaging device is mounted on the frame and located on one side of the sample mounting device. The shielding member is movably mounted on the frame. When the shielding member is in a first position, the sample can be mounted onto the sample mounting device through the sample mounting port. When the shielding member is in a second position, the shielding member blocks the sample mounting path corresponding to the sample mounting port.

[0007] In some embodiments, when the shielding member is in the second position, the surface of the shielding member facing the sample mounting port is the first surface; the first surface is provided with elongated ribs, and when the shielding member is in the first position, the extending direction of the elongated ribs is consistent with the sample mounting path.

[0008] In some embodiments, the frame includes a mounting frame having a first opening for sample passage; one end of a shield is pivotally connected to the mounting frame at the top of the first opening; the shield is capable of being raised away from the first opening or lowered towards the first opening.

[0009] In some embodiments, the sample mounting device includes a sample clamp and a clamp driving component. The sample clamp is movably mounted on a frame; the clamp driving component is mounted on the frame and connected to the sample clamp, and is used to drive the sample clamp to move along a sample mounting path.

[0010] In some embodiments, the sample fixture has a mounting space for mounting a sample. The sample fixture includes a first clamping structure and a second clamping structure. The first clamping structure is located at the edge of the mounting space, and the second clamping structure is located at the top and / or bottom of the mounting space. The clamping directions of the first clamping structure and the second clamping structure are perpendicular to each other, both facing the center of the mounting space, and both perpendicular to the sample mounting path.

[0011] In some embodiments, the sample fixture has a mounting space for mounting a sample, with the sample mounting port located at one end of the mounting space; the sample fixture includes a sample positioning sensor located at the other end of the mounting space.

[0012] In some embodiments, the sample fixture is provided with multiple observation through holes, which are arranged along the straight line of the sample mounting path.

[0013] In some embodiments, the sample mounting device further includes a position sensor mounted on the frame, which is used to detect whether the sample holder is in a limit movement position.

[0014] In some embodiments, the imaging apparatus includes an objective lens and an objective lens drive motor. The objective lens is movably mounted on a frame; the objective lens drive motor is mounted on the frame and is drively connected to the objective lens, and the objective lens drive motor is used to drive the objective lens to move along a straight line perpendicular to the sample mounting path.

[0015] The second aspect of this application provides a workstation, which includes a housing, a robotic arm, and a cell imaging device provided in any embodiment of the first aspect of this application. The cell imaging device is disposed within the housing; the robotic arm is disposed within the housing and is used to grasp a sample and load the sample onto a sample mounting device via a sample mounting port.

[0016] The advantages of this application compared to the prior art are:

[0017] The cell imaging device provided in this application embodiment enables normal sample installation and removal through a movable shielding component, effectively protecting the sample and sample mounting device during operation. When the shielding component is in the first position, the sample mounting port is exposed, allowing operators to efficiently and conveniently insert the sample into the sample mounting device via the port. When the shielding component is in the second position, the sample mounting port is blocked, or the sample mounting path on one side of the port is obstructed, preventing operators from inserting the sample into the device. This effectively protects the sample and sample mounting components during cell imaging, preventing accidental insertion or removal of the sample, thereby reducing the risk of equipment damage and improving the reliability of the cell imaging device. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the workstation structure shown in some embodiments of this application;

[0020] Figure 2 This is a schematic diagram of the overall structure of a cell imaging device shown in some embodiments of this application;

[0021] Figure 3 This is a front view schematic diagram of a cell imaging device shown in some embodiments of this application;

[0022] Figure 4 This is a side view schematic diagram of a cell imaging device shown in some embodiments of this application;

[0023] Figure 5 The following are schematic diagrams illustrating the structure of the shielding member in some embodiments of this application;

[0024] Figure 6 This is a schematic diagram showing the shielding member in a second position as illustrated in some embodiments of this application;

[0025] Figure 7 This is a partial structural schematic diagram of a sample mounting device shown in some embodiments of this application;

[0026] Figure 8 This is a partial structural schematic diagram of a sample mounting device shown in some embodiments of this application;

[0027] Figure 9 This is an exploded view of a sample fixture shown in some embodiments of this application;

[0028] Figure 10 This is a schematic diagram showing the shielding member in a first position according to some embodiments of this application;

[0029] Figure 11 This is a schematic diagram showing the shielding member in a first position as illustrated in some embodiments of this application;

[0030] Figure 12 This is a partial structural schematic diagram of an imaging device shown in some embodiments of this application;

[0031] Figure 13 This is a top view schematic diagram illustrating a fluorescence switching component in some embodiments of this application;

[0032] Figure 14 This is a top view schematic diagram of an optical path switching turntable shown in some embodiments of this application.

[0033] Icons: 1-Workstation; 100-Sample; 2-Robotic arm; 4-Cell imaging equipment; 5-Rack; 51-Mounting frame; 52-Aluminum plate support; 53-Sample mounting plate; 54-Support base; 510-First opening; 6-Sample mounting device; 60-Mounting space; 600-Sample mounting port; 61-Sample clamp; 611-Clamp top plate; 612-Clamp bottom plate; 613-First clamping structure; 614-Second clamping structure; 615 - Sample positioning sensor; 62- Fixture drive component; 621- Fixture drive motor; 622- Fixture connector; 623- Fixture transmission mechanism; 63- Position sensor; 64- Observation through hole; 65- Guide mechanism; 7- Light source device; 71- Bright field component; 711- Bright field light source assembly; 7111- Bright field circuit board; 7112- Bright field LED; 7113- Bright field lens; 7114- Bright field reflector; 712- Bright field light blocking assembly; 7 2-Fluorescence switching component; 721-Fluorescence excitation light source assembly; 7211-Fluorescence excitation light source unit; 722-Fluorescence emission light filter assembly; 7221-Fluorescence emission light filter unit; 723-Fluorescence circuit board; 73-Optical path switching turntable; 731-Bright field through-hole; 732-Fluorescence through-hole; 733-Turntable drive unit; 8-Imaging device; 81-Objective lens assembly; 811-Objective lens; 812-Objective lens drive motor; 813-Objective lens drive motor; Mirror connector; 82-Imaging component; 821-Camera; 822-Imaging lens barrel; 823-Imaging mirror; 90-Shielding component; 901-First surface; 902-Long rib; 91-Core plate; A1-First position; A2-Second position; B1-Sample mounting path; B2-Sample disassembly path; C1-Clamping direction of the first clamping structure; C2-Clamping direction of the second clamping structure; D1-Bright field imaging optical path; D2-Fluorescence imaging optical path. Detailed Implementation

[0034] The terms “first,” “second,” “third,” etc., are used only for distinguishing descriptions and do not indicate a sequence number, nor should they be interpreted as indicating or implying relative importance.

[0035] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0036] In the description of this application, it should be noted that the terms "inner", "outer", "left", "right", "upper", "lower", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this application is in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0037] In the description of this application, unless otherwise expressly specified and limited, the terms “set up,” “install,” “connect,” and “link” shall be interpreted broadly, for example, as a fixed connection, a detachable connection, or an integral connection; as a mechanical connection or an electrical connection; as a direct connection or an indirect connection through an intermediate medium; or as a connection within two components.

[0038] The technical solution of this application will now be described in detail with reference to the accompanying drawings.

[0039] Please see Figure 1 , Figure 1 This is a schematic diagram of the structure of workstation 1 shown in some embodiments of this application. For example... Figure 1 As shown, this application embodiment provides a workstation 1, which includes a housing, a robotic arm 2, and a cell imaging device 4. The cell imaging device 4 and the robotic arm 2 are both located inside the housing or on the housing. The robotic arm 2 is used to grasp a sample 100 and load the sample to be counted or tested onto the sample mounting device 6 via the sample mounting port 600 on the cell imaging device 4.

[0040] In this embodiment, workstation 1 typically refers to an integrated equipment platform designed to complete specific biomedical detection or cell analysis tasks, usually including multiple multifunctional devices such as sample processing and imaging analysis; housing typically refers to the physical shell of workstation 1, used to accommodate, support, cover or protect the various functional devices of workstation 1, while providing an operating interface; robotic arm 2 typically refers to an automatic actuator that can accurately grasp, transport and position sample 100 through joint movement; cell imaging device 4 typically refers to a professional device used for optical imaging of cell samples, capable of performing operations such as fluorescent labeling, acquiring cell morphology images, and cell counting.

[0041] In the above technical solution, workstation 1 can reduce human error and improve detection efficiency through the collaborative work of robotic arm 2 and cell imaging device 4, making it suitable for high-throughput analysis of large-scale, multi-demand samples. Robotic arm 2 can move along multiple degrees of freedom based on preset programs or real-time control commands to complete the sample transfer process of "grabbing → transferring → installing / placing", thereby reducing the probability of contamination or damage caused by human contact with samples. Cell imaging device 4, combined with automated robotic arm, can realize fully automated operation of "sample in - result out", improving the detection and analysis efficiency and work intelligence of workstation 1.

[0042] Please see Figures 2 to 4 , Figure 2 This is a schematic diagram of the overall structure of the cell imaging device 4 shown in some embodiments of this application; Figure 3 This is a front view schematic diagram of the cell imaging device 4 shown in some embodiments of this application; Figure 4 This is a side view schematic diagram of a cell imaging device 4 shown in some embodiments of this application. For example... Figures 2 to 4 As shown, this application embodiment provides a cell imaging device 4, including a frame 5, a sample mounting device 6, a light source device 7, an imaging device 8, and a shielding member 90. The sample mounting device 6 is mounted on the frame 5 and includes a sample mounting port 600; the light source device 7 is mounted on the frame 5 and can provide illumination light to the sample 100 on the sample mounting device 6; the imaging device 8 is mounted on the frame 5 and located on one side of the sample mounting device 6; the shielding member 90 is movably mounted on the frame 5.

[0043] In this embodiment, the frame 5 typically refers to the main support structure of the cell imaging device 4, used to fix, connect, and support various functional devices, such as the sample mounting device 6, the light source device 7, and the imaging device 8; the sample mounting device 6 typically refers to the structure used to fix or position the sample 100, so that the sample 100 is stably in a constant position or constant region (a region that can reciprocate) during imaging; the sample mounting port 600 typically refers to the physical interface through which the sample 100 enters the cell imaging device 4 and is loaded onto the sample mounting device 6. The sample mounting port 600 is typically an opening or slot structure, allowing the robotic arm 2 or the operator to place the sample 100 into the sample mounting device 6. 00 (such as sample plate, sample chip, glass slide, culture dish) is placed into the designated position for imaging; the light source device 7 usually refers to the device that can provide illumination or fluorescence excitation light for cell sample imaging. The light source device 7 can project light of different wavelengths and intensities to make cell structures or markers clearly visible; the imaging device 8 usually refers to the device that can adjust the focal length and convert optical signals into digital images, and is a key device for cell imaging; the blocking component 90 refers to the part that can prevent the sample 100 from being loaded into the sample mounting device through the sample mounting port 600, or the part that can prevent the sample 100 from being removed from the sample mounting device through the sample mounting port 600.

[0044] Please see Figures 5 to 6 , Figure 5 This is a schematic diagram of the structure of the shielding member 90 shown in some embodiments of this application; Figure 6 This is a schematic diagram showing the shielding member 90 in the second position A2, as illustrated in some embodiments of this application. Please refer to... Figures 2 to 6 As shown, when the shielding member 90 is in the second position A2, the shielding member 90 blocks the sample mounting path B1 or sample disassembly path B2 corresponding to the sample mounting port 600; please refer to... Figures 10 to 11 As shown, when the shielding member 90 is in the first position A1, the sample 100 can be mounted onto the sample mounting device 6 via the sample mounting port 600.

[0045] In this embodiment, the first position A1 and the second position A2 can be relative, for example, closer to / farther from the sample mounting port; the first position A1 and the second position A2 can also be fixed, respectively corresponding to the two extreme positions of the movable range of the shielding member 90, such as retracting / extending to a position where it can no longer move, rotating forward / reverse to a position where it can no longer rotate, etc. Specifically, the normal projection path of the sample mounting port 600 is used as the sample mounting path B1 or the sample disassembly path B2. The first position A1 can refer to a position that is further away from the sample mounting path B1 or the sample disassembly path B2. In the case of the first position A1, the blocking member 90 has no or almost no overlapping area with the sample mounting path B1 or the sample disassembly path B2, allowing the sample 100 to be smoothly installed into or removed from the sample mounting device 6 through the sample mounting port 600. The second position A2 can refer to a position that is closer to the sample mounting path B1 or the sample disassembly path B2, and has a significant overlapping or conflicting area with the sample mounting path B1 or the sample disassembly path B2. In the case of the second position A2, the blocking member 90 usually blocks or covers the sample mounting port 600, or exists as an obstacle on the sample mounting path B1 or the sample disassembly path B2, preventing the sample 100 from entering or leaving. When the cell imaging device 4 is in standby mode or during sample loading and unloading, the shielding component 90 can be passively (pushed away, rotated or moved by the operator) or actively (e.g., through the connection and drive of a corresponding drive motor) switched to the first position A1; when the cell imaging device 4 is in the imaging process, the shielding component 90 can be passively (pushed away, rotated or moved by the operator) or actively (e.g., falling due to its own gravity or through the connection and drive of a corresponding drive motor) switched to the second position A2.

[0046] In the above technical solution, the cell imaging device 4 uses a movable shielding member 90 to enable normal installation and removal of the sample 100 and to effectively protect the sample 100 and the sample mounting device 6 during operation. When the shielding member 90 is in the first position A1, the sample mounting port 600 is exposed, allowing the operator to efficiently and conveniently load the sample 100 into the sample mounting device 6 through the port. When the shielding member 90 is in the second position A2, the sample mounting port 600 is blocked, preventing the operator from loading or removing the sample 100 from the device. This ensures effective protection for the sample 100 and the sample mounting device 6 during cell imaging. Related cell imaging devices 4 often lack protective mechanisms for the sample 100 and the instrument, posing significant safety hazards and potentially affecting experimental progress. The cell imaging device 4 provided in this embodiment reduces the risk of equipment damage and improves operational and application reliability.

[0047] In some embodiments, when the shielding member 90 is in the second position A2, the surface of the shielding member 90 facing the sample mounting port 600 is a first surface 901; the first surface 901 is provided with a long rib 902, and when the shielding member 90 is in the first position A1, the extending direction of the long rib 902 is consistent with the sample mounting path B1.

[0048] In this embodiment, the sample installation path B1 typically refers to a predetermined fixed direction or trajectory during the assembly of the sample 100, and the sample disassembly path B2 typically refers to a predetermined fixed direction or trajectory during the removal of the sample 100; the extension direction of the long rib 902 typically refers to the geometric extension trend or functional direction of the main body of the long rib 902; the extension direction of the long rib 902 being consistent with the sample installation path B1 or the sample disassembly path B2 not only means that the extension direction of the long rib 902 is absolutely parallel to the sample installation path B1 or the sample disassembly path B2, but also means that the angle between the main extension direction of the long rib 902 and the straight line direction of the sample installation path B1 or the sample disassembly path B2 is an acute angle, for example, with Figures 2 to 4 For example, the straight line direction corresponding to the sample installation path B1 and the sample disassembly path B2 is the X direction. When the shielding part 90 is in the first position A1, the long strip 902 is projected along the Z direction (perpendicular to the X direction, which can be regarded as the vertical height direction of the cell imaging device 4). The projection in the X direction still has an effective extension length greater than 0.

[0049] Specifically, two elongated ribs 902 can be provided. When the elongated ribs 902 are in the first position A1, the projections of the two elongated ribs 902 along the Z direction can be located on both sides of the sample observation area on the sample 100. When the sample 100 is inserted into the sample mounting port 600, the sample observation area is in the horizontal direction perpendicular to the sample mounting path B1. Figures 2 to 4 The maximum distance in the Y direction (as shown) is used as the width of the sample observation area. The spacing between the two long strips 902 should be greater than the width of the sample observation area.

[0050] In the above technical solution, the elongated rib 902 is disposed on the surface of the shield 90 facing the sample mounting port 600 when the shield 90 is in the second position A2, so that when the sample 100 enters or leaves the sample mounting device 6 through the sample mounting port 600, it preferentially contacts the elongated rib 902. When the sample 100 leaves the sample mounting device 6, the elongated rib 902 can push the shield 90 away, so that the shield 90 switches to the first position A1, which no longer obstructs the sample removal path B2. At this time, the extension direction of the elongated rib 902 is consistent with the sample mounting path B1 or the sample removal path B2, so that the shield 90 and the sample 100 move relative to each other in the form of line contact. This can reduce the contact area between the shield 90 and the sample 100, realize the weight reduction of the entire shield 90, and thus enable the shield 90 to support the anti-misinsertion function while reducing scratches and wear on the sample 100, protecting the sample 100 and making the observation field clearer and cleaner.

[0051] In some embodiments, the frame 5 includes a mounting frame 51 and two vertically arranged aluminum plate supports 52. The two aluminum plate supports 52 are arranged in an L-shape and connected to each other. The mounting frame 51 is connected to one of the aluminum plate supports 52. The mounting frame 51 has a first opening 510 for the sample 100 to pass through. One end of the shield 90 is pivotally connected to the mounting frame 51, and the pivot position is located at the top of the first opening 510. The shield 90 can be raised in a direction away from the first opening 510 or lowered in a direction close to the first opening 510.

[0052] Specifically, the mounting bracket 51 can be a rectangular sheet metal part. A first opening 510 is provided in the middle of the mounting bracket 51, which can be a rectangular through hole. The first opening 510 allows the sample 100 (or a clamp used to fix the sample 100) to pass through without interference. Furthermore, the mounting bracket 51 is provided with a fixing block along... Figures 2 to 4 The Z-direction fixing block shown is located at the top of the first opening 510 and is positioned relative to the mounting bracket 51 in the direction indicated by the sample disassembly path B2. The shielding member 90 can be pivotally connected to the fixing block by connecting screws or pivots, and the pivoting position is located at the top of the first opening 510. The long rib 902 is located on the surface of the shielding member 90 facing the first opening 510.

[0053] In the above technical solution, one end of the shielding member 90 is pivotally connected to the top of the first opening 510, so that when the shielding member 90 is not subjected to external force, it can automatically return to the second position A2 that can shield the first opening 510 based on its own gravity. This ensures that the cell imaging device 4 always maintains a state of preventing accidental insertion and removal when the sample 100 or the sample holder 61 is not extended out of the first opening 510, thereby improving the reliability of the cell imaging device 4.

[0054] Please see Figures 7 to 9 , Figure 7This is a partial structural schematic diagram of the sample mounting device 6 shown in some embodiments of this application; Figure 8 This is a partial structural schematic diagram of the sample mounting device 6 shown in some embodiments of this application; Figure 9 This is an exploded view of the sample fixture 61 shown in some embodiments of this application. Please refer to... Figures 2 to 9 As shown, the sample mounting device 6 may include a sample clamp 61 and a clamp driving component 62. The sample clamp 61 is movably mounted on the frame 5, and the clamp driving component 62 is also mounted on the frame 5 and connected to the sample clamp 61. The clamp driving component 62 is used to drive the sample clamp 61 along the sample mounting path B1 or the sample disassembly path B2 (i.e., ...). Figures 2 to 4 Move in the X direction (as shown).

[0055] Specifically, the frame also includes a sample mounting plate 53, which is mounted on an aluminum plate support 52. The sample mounting plate 53 can also be L-shaped. Furthermore, a sample clamp 61 is movably mounted on the horizontal plate of the sample mounting plate 53, and a clamp driving component 62 is mounted on the vertical plate of the sample mounting plate 53. The output end of the clamp driving component 62 passes through the vertical plate of the sample mounting plate 53 and connects to the sample clamp 61.

[0056] Specifically, the fixture driving component 62 may include a fixture driving motor 621, a fixture connector 622, and a fixture transmission mechanism 623. The fixture driving motor 621 may be mounted on an L-shaped fixed plate. The output end of the fixture driving motor 621 can be connected to the fixture connector 622 via the fixture transmission mechanism 623 to drive the fixture connector 622 to reciprocate along the sample mounting path B1 or the sample disassembly path B2. The fixture transmission mechanism 623 may be a lead screw and nut mechanism. The fixture connector 622 is used to connect the sample fixture 61. The fixture connector 622 can be connected to the L-shaped fixed plate via a guide mechanism 65. The guide mechanism 65 may be a cross roller guide to save space.

[0057] Specifically, the sample fixture 61 may include a fixture top plate 611 and a fixture bottom plate 612. The fixture top plate 611 is configured to mate with and connect to the fixture bottom plate 612 to form a mounting space 60 for mounting and accommodating the sample 100. The fixture bottom plate 612 may be fixed to the fixture connector 622 to move together with the fixture connector 622. One end of the mounting space 60 communicates with the outside as a sample mounting port 600. The sample mounting port 600 is typically located at the end of the mounting space 60 away from the fixture drive motor 621 or the fixture transmission mechanism 623.

[0058] In the above technical solution, the sample clamp 61 holding the sample 100 can achieve fully automatic displacement under the drive of the clamp driving component 62. It is suitable for application scenarios where the sample 100 has multiple sample observation areas or multiple sample slots, and needs to be moved multiple times and accurately during the imaging process to realize multi-sample area imaging and photography. This enables fully automatic counting and related detection of cell samples inside the cell imaging device 4, and can improve the automation level and working efficiency of the cell imaging device 4.

[0059] In some embodiments, the sample mounting device 6 further includes a position sensor 63, which is disposed on the sample mounting plate 53. The position sensor 63 is used to detect whether the sample clamp 61 is at its limit movement position. In this embodiment, the position sensor 63 can monitor the movement position of the sample clamp 61 or the clamp connector 622 in real time. When the sample clamp 61 or the clamp connector 622 approaches a preset limit position (such as the end of the stroke or reaching the minimum safe distance from components such as motors) along the sample mounting path B1 or the sample disassembly path B2, the position sensor 63 immediately sends a signal to the clamp driving component 62 to trigger an emergency stop mechanism or a deceleration buffer program. Specifically, the position sensor 63 can be disposed on the L-shaped fixing plate and located at the end adjacent to the clamp driving motor 621. When the sample clamp 61 or the clamp connector 622 moves to its limit along the sample mounting path B1, the position sensor 63 is triggered, and the clamp driving motor 621 controls the sample clamp 61 or the clamp connector 622 to stop moving.

[0060] In the above technical solution, the cell imaging device 4, through the setting of the position sensor 63, can realize closed-loop control of the sample clamp 61 or clamp connector 622, thereby improving the reliability of the movement of the sample clamp 61 or clamp connector 622.

[0061] In some embodiments, the sample clamp 61 is provided with a mounting space 60 for mounting a sample 100, and a sample mounting port 600 is located at one end of the mounting space 60; the sample clamp 61 includes a sample positioning sensor 615, which is located at the other end of the mounting space 60.

[0062] Specifically, the sample holder 61 can be fixed to the holder connector 622 with screws. A sample positioning sensor 615 is provided at the end of the holder away from the sample mounting port 600. When the sample 100 is inserted into the predetermined position through the sample mounting port 600, the sample 100 will push the corresponding identification structure into the sample positioning sensor 615. The sensor triggers and sends a signal indicating that the sample 100 has been inserted into position, allowing the cell imaging device 4 to proceed to subsequent operation steps. In the above technical solution, the sample positioning sensor 615 improves the accuracy of the sample holder 61 in positioning the sample 100, thereby improving the efficiency and quality of cell imaging.

[0063] In some embodiments, the sample clamp 61 is provided with a mounting space 60 for mounting the sample 100. The sample clamp 61 includes a first clamping structure 613 and a second clamping structure 614. The first clamping structure 613 is located at the edge of the mounting space 60, and the second clamping structure 614 is located at the top and / or bottom of the mounting space 60. The clamping direction C1 of the first clamping structure and the clamping direction C2 of the second clamping structure are perpendicular to each other, both facing the center of the mounting space 60, and both perpendicular to the sample mounting path B1.

[0064] In this embodiment, the first clamping structure 613 can be disposed on the top plate 611 and / or the bottom plate 612 of the fixture, and the second clamping structure 614 can be disposed on the bottom plate 612 of the fixture. Both the first clamping structure 613 and the second clamping structure 614 are used to position and fix the sample 100 inserted into the mounting space 60. Specifically, the first clamping structure 613 is arranged at the edge of the mounting space 60, and its clamping direction is perpendicular to the sample mounting path B1. For rectangular sample plates (such as sample detection chips), this can reduce the lateral movement or tilting of the sample 100 during the installation and removal process (which can be understood as the sample 100 in...). Figures 2 to 4 To reduce the probability of the sample 100 moving along the sample installation path B1 or sample removal path B2, multiple first clamping structures 613 can be provided and evenly distributed on both sides of the installation space 60; the second clamping structure 614 is provided at the top and / or bottom of the installation space 60, and its clamping direction is perpendicular to the sample installation path B1 and orthogonal to the clamping direction C1 of the first clamping structure, which can restrict the sample 100 from moving along the sample installation path B1 or sample removal path B2, or even from detaching from the sample clamp 61.

[0065] In the above technical solution, the pressing directions of the first pressing structure and the second pressing structure are perpendicular to each other and are both perpendicular to the sample installation path B1 or the sample disassembly path B2, so that the sample 100 can be subject to more directional constraints within the installation space 60, thereby improving the positioning stability and positioning accuracy of the sample clamp 61 for the sample 100, and thus improving the imaging quality of the cell imaging device 4.

[0066] In some embodiments, the sample fixture 61 is provided with a plurality of observation through holes 64, which are arranged along the straight line direction of the sample installation path B1. In this embodiment, the fixture top plate 611, the fixture bottom plate 612, and the fixture connector 622 are all provided with a plurality of observation through holes 64 of the same size and shape, equal number, consistent spacing, and consistent arrangement direction. After the fixture top plate 611, the fixture bottom plate 612, and the fixture connector 622 are assembled and connected, the observation through holes 64 on the fixture top plate 611, the fixture bottom plate 612, and the fixture connector 622 are aligned one by one to form a plurality of sample observation ports. Specifically, there may be five sample observation ports. After the sample fixture 61 and the fixture connector 622 are connected and assembled, the arrangement direction of the sample observation ports is aligned with the straight line direction of the sample installation path B1 or the sample removal path B2 (i.e., Figures 2 to 4 (shown in the X direction) are consistent; the shape and number of the sample observation ports can also be consistent with the multiple sample observation slots or sample observation areas on the sample 100. After the sample 100 is installed on the sample fixture 61, the multiple sample observation slots and sample observation ports can be aligned one by one.

[0067] During operation, the fixture connector 622, driven by the fixture drive motor 621, moves the sample fixture 61 and the sample 100 (which has already been installed) along the sample installation path B1 or the sample removal path B2, placing the observation port of the sample to be observed in the imaging optical path, making the corresponding optical axis vertical (along the...). Figures 2 to 4 The light path (in the Z direction as shown) passes through the sample observation port to complete the observation and photography of the cell sample in the sample observation port. As the sample clamp 61 and sample 100 (sample 100 is already installed) move along the sample installation path B1 or the sample removal path B2, the optical path used for imaging can pass through each sample observation port in sequence, and take multiple sample images for each sample observation port according to the actual imaging requirements. Then, the final cell image is generated by the algorithm and used for detection analysis or technology.

[0068] Please see Figures 10 to 11 , Figure 10 and Figure 11 This is a schematic diagram showing the shielding member 90 in the first position A1, as illustrated in some embodiments of this application. Figure 10 and Figure 11 The diagrams illustrate the contact between the shielding member 90 and the sample 100 or the sample clamp 61 in two states: during the movement of the sample clamp 61 and when the sample clamp 61 is fully extended along the sample disassembly path B2 through the first opening 510. Please refer to the diagrams provided. Figure 6 , Figure 10 and Figure 11As shown, after the sample 100 and sample holder 61 are fully retracted along the sample mounting path B1 through the first opening 510, the shielding member 90 is fully lowered and in the second position A2. The shielding member 90 blocks the sample mounting port 600 of the sample holder 61, reducing the probability of the sample mounting device 6 being accidentally inserted into an interference source during cell imaging. During the movement of the sample holder 61, the long rib 902 of the first surface 901 of the shielding member 90 is provided, so that the shielding member 90 moves relative to the sample 100 in a line contact manner, reducing the probability of the shielding member 90 scratching and wearing the sample 100. When the sample holder 61 is fully extended along the sample disassembly path B2 through the first opening 510, the shielding member 90 moves relative to the sample holder 61 in a line contact manner, reducing the probability of the shielding member 90 scratching and wearing the top plate 611 of the holder. In the above technical solution, the relative motion of the line contact reduces the resistance between the shield 90 and the sample 100 or the sample clamp 61, making it easier for the sample clamp 61 or the sample 100 to turn from stationary to moving again.

[0069] Furthermore, the position design of the mounting bracket 51 (shielding member 90) should ensure that when the sample clamp 61 moves to switch the sample observation port, the shielding member 90 can always block the sample installation port 600 or prevent the installation or removal of the sample 100; the maximum movable stroke of the sample clamp 61 can be greater than the maximum stroke for switching the sample observation port, so that the sample clamp 61 can extend further along the sample removal path B2 through the first opening 510 and push the shielding member 90 to the first position A1 to expose the sample installation port 600 for sample installation or removal.

[0070] Please see Figure 12 , Figure 12 This is a partial structural schematic diagram of the imaging device 8 shown in some embodiments of this application. For example... Figure 12 As shown, in some embodiments, the imaging device 8 may include an objective lens assembly 81 and an imaging assembly 82. The objective lens assembly 81 may include an objective lens 811, an objective lens drive motor 812, and an objective lens connector 813. The objective lens 811 is movably mounted on the aluminum plate support 52 via a guide rail slider. The objective lens drive motor 812 is mounted on the aluminum plate support 52 and can be connected to the objective lens 811 via the objective lens connector 813, either through transmission or direct connection. The objective lens drive motor 812 is used to drive the objective lens 811 along a straight line direction perpendicular to the sample mounting path B1 (e.g., ...). Figures 2 to 4 The cell imaging device shown moves in the vertical height direction (Z direction).

[0071] Specifically, the objective lens 811 may include an objective lens barrel and a first achromatic lens and a second achromatic lens disposed within the objective lens barrel. The first achromatic lens and the second achromatic lens can be along... Figures 2 to 4 The Z-direction (vertical height direction) is set sequentially from top to bottom as shown.

[0072] In the above technical solution, the cell imaging device 4 can use the objective lens drive motor 812 to move the objective lens 811 vertically up and down, so as to realize the automatic focusing and focusing of the imaging device 8 on the cell sample in the sample 100; the guide rail slider can guide the objective lens 811 along the vertical direction. Figures 2 to 4 The objective lens 811 moves up and down in the Z direction (vertical height direction) to ensure that the imaging optical path corresponding to the objective lens 811 is always aligned with the cell sample in the sample observation port. This embodiment of the application realizes the automatic focusing and adjustment function of the cell imaging device 4, improving cell imaging efficiency, cell imaging quality, and the level of automation in cell imaging.

[0073] Please combine Figures 2 to 12 As shown, the imaging component 82 may include a camera 821, an imaging barrel 822, an imaging lens, an imaging mirror 823, and a light-blocking sheet metal. Please refer to... Figures 2 to 4 As shown, along the vertical height direction Z of the cell imaging device 4, the camera 821, imaging tube 822, imaging lens, imaging mirror 823, and light-blocking sheet metal are all located at the bottom of the objective lens component 81. The light-blocking sheet metal is located around the camera 821, imaging tube 822, imaging lens, and imaging mirror 823 to block light. The imaging lens can be located inside the imaging tube 822. The camera 821 and imaging mirror 823 are located on both sides of the imaging tube 822. The imaging mirror 823 is located at the intersection of the imaging optical path of the objective lens 811 and the imaging optical path of the camera 821. The tilt of the imaging mirror 823 is usually determined based on the position and angle between the two imaging optical paths, that is, the normal corresponding to the reflecting surface of the imaging mirror 823 is between the imaging optical path of the imaging lens (camera 821) and the imaging optical path of the objective lens 811, and evenly divides the angle formed by the imaging optical path of the objective lens 811 and the imaging optical path of the camera 821. Furthermore, when the angle between the imaging optical path where the objective lens 811 is located and the imaging optical path where the camera 821 is located is a right angle, the tilt angle of the imaging mirror 823 relative to any imaging optical path is 45°.

[0074] The light source device 7 may include a bright field component 71 and a fluorescence switching component 72. For example, Figure 12As shown, the bright-field component 71 may include a bright-field light source assembly 711 and a bright-field light-blocking assembly 712. The bright-field light source assembly 711 includes a bright-field circuit board 7111, a bright-field LED bead 7112, a bright-field lens 7113, and a bright-field reflector 7114. Specifically, the bright-field lens 7113 may include a bright-field plano-convex lens and a bright-field collimating lens. The bright-field reflector 7114 is located at the intersection of the illumination light path where the bright-field lens 7113 is located and the imaging light path where the objective lens 811 is located, and its arrangement is similar to that of the imaging reflector 823. The tilt of the bright-field reflector 7114 is usually determined based on the position and angle between the two light paths, that is, the normal corresponding to the reflective surface of the bright-field reflector 7114 lies between the illumination light path where the bright-field lens 7113 is located and the imaging light path where the objective lens 811 is located, and evenly divides the angle formed by the imaging light path where the objective lens 811 is located and the illumination light path where the bright-field lens 7113 is located. Furthermore, when the angle between the imaging optical path where the objective lens 811 is located and the illumination optical path where the bright field lens 7113 is located is a right angle, the tilt angle of the bright field mirror 7114 relative to either optical path is 45°.

[0075] The bright-field light-blocking component 712 is used to selectively transmit or block bright-field light beams. The bright-field light-blocking component 712 can be driven by a motor or electromagnet. The shape of the light-blocking plate can be designed as a disc or rectangle according to requirements. When the bright-field light-blocking component 712 is in the light-blocking state, the light-blocking plate is located between the bright-field reflector 7114 and the sample mounting device 6. The bright-field light beam, after being reflected by the bright-field reflector 7114, is blocked by the light-blocking plate and cannot pass through the sample observation port, thus achieving the blocking of bright-field light.

[0076] In one application, when the bright-field light-blocking component 712 is in a light-transmitting state, the bright-field circuit board 7111 receives a lighting signal and controls the bright-field LED chip 7112 to light up. The bright-field beam emitted by the bright-field LED chip 7112 reaches the bright-field reflector 7114 via the bright-field lens 7113, and after being reflected by the bright-field reflector 7114, it travels along... Figures 2 to 4 The light rays in the Z direction vertically penetrate the sample observation port and the objective lens 811 from top to bottom, and then illuminate the imaging mirror 823. After being reflected by the imaging mirror 823, the light rays reach the imaging lens and the camera 821. The camera 821 can then perform bright-field imaging of the cell sample in the sample observation port. The light path corresponding to the above light transmission path is the bright-field imaging light path D1.

[0077] Please see Figures 13 to 14 , Figure 13 This is a top view schematic diagram of the fluorescence switching component 72 shown in some embodiments of this application; Figure 14 This is a top view schematic diagram of the optical path switching turntable 73 shown in some embodiments of this application. Please refer to... Figures 2 to 4 , Figures 13 to 14As shown, the fluorescence switching component 72 may include a fluorescence excitation light source assembly 721, a fluorescence emission light filter assembly 722, and a fluorescence circuit board 723. The fluorescence switching component 72 is mounted on the frame 5 and extends along... Figures 2 to 4 The vertical height direction Z of the cell imaging device 4 shown is located between the objective lens component 81 and the imaging component 82.

[0078] Specifically, the frame also includes a support base 54 located at the bottom of the aluminum plate bracket 52, and the fluorescence switching component 72 is mounted on the support base 54. Further, a fluorescence excitation light source assembly 721 is fixedly mounted on the support base 54, and the fluorescence excitation light source assembly 721 includes multiple fluorescence excitation light source units 7211; a fluorescence circuit board 723 is electrically connected to the fluorescence excitation light source units 7211, used to drive the fluorescence excitation light source units 7211 to light up and emit different fluorescence excitation light toward the fluorescence emission filter assembly 722. The light source device may also include an optical path switching turntable 73, which is rotatably mounted on the support base 54 and has a bright-field through-hole 731 and multiple fluorescence through-holes 732, which can be arranged circumferentially on the optical path switching turntable 73.

[0079] The optical path switching turntable 73 can be driven to rotate by the turntable drive unit 733. During the rotation of the optical path switching turntable 73, the bright field aperture 731 or any fluorescence aperture 732 can reach the top (or directly above) of the light-transmitting hole on the support base 54 and be located on the imaging optical path of the objective lens 811. The imaging mirror 823 is located at the bottom (directly below) of the light-transmitting hole. The fluorescence emission filter assembly 722 includes multiple fluorescence emission filter units 7221, all of which are located on the optical path switching turntable 73. Each fluorescence emission filter unit 7221 is located on top of a fluorescence aperture 732. The fluorescence emission filter unit 7221 can rotate with the optical path switching turntable 73 and can be selectively switched to be directly above the light-transmitting hole. The fluorescent emission filter unit 7221 and the fluorescent excitation light source unit 7211 can correspond one-to-one. Each fluorescent excitation light source unit 7211 can emit fluorescent excitation light toward the fluorescent emission filter unit 7221 located at the top of the light-transmitting hole.

[0080] Furthermore, the fluorescence excitation light source unit 7211 may include a heat sink, a fluorescence light source, a plano-convex lens, and a fluorescence collimating lens arranged sequentially; the fluorescence emission light filter unit 7221 may include a mounting base and a dichroic mirror and an emission filter disposed within the mounting base. Five sets of fluorescence through-holes 732, fluorescence excitation light source units 7211, and fluorescence emission light filter units 7221 may be provided to respectively realize five different types of fluorescence excitation light emission and fluorescence imaging.

[0081] In one application, the turntable drive unit 733 drives the optical path switching turntable 73 to rotate to a designated position. A fluorescence emission filter unit 7221 rotates to the position directly above the light transmission hole. The fluorescence excitation light emitted by the fluorescence excitation light source unit 7211 corresponding to the fluorescence emission filter unit 7221 is filtered and reflected by the fluorescence emission filter unit 7221 and then transmitted upward along the imaging optical path of the objective lens 811 to the sample observation port. The cell sample in the sample observation port is excited by the fluorescence excitation light and produces fluorescence. Subsequently, the fluorescence produced by the sample is transmitted in the reverse direction and is projected onto the imaging mirror 823 along the imaging optical path of the objective lens 811, through the objective lens 811, the fluorescence emission filter unit 7221, the fluorescence through hole 732 and the light transmission hole. After being reflected by the imaging mirror 823, it reaches the imaging lens and the camera 821. The camera 821 can then perform fluorescence imaging on the cell sample emitting fluorescence in the sample observation port. The optical path corresponding to the above light transmission process is the fluorescence imaging optical path D2.

[0082] In some embodiments, the cell imaging device 4 may also include components such as a fan, motherboard, core board 91 and connectors mounted on the rack 5 to achieve functions such as heat dissipation, power supply, data transmission and algorithm control.

[0083] The cell imaging device 4 provided in this embodiment can be used as a fully automated high-throughput cell counter. For some cell samples that can be imaged without staining, the cell imaging device 4 can achieve bright-field imaging. The light source of the cell imaging device 4 is provided by a bright-field light source component 711 and a fluorescence excitation light source component 721. The two light sources can be lit alternately to complete bright-field imaging or fluorescence field imaging of cell samples, which has a wider range of applications and meets different experimental needs. Driven by the clamp drive motor 621, the sample clamp 61 can automatically move the porous sample 100 in and out, thereby realizing fully automated imaging, accurate counting and detection analysis of multiple cell samples, simplifying manual operation steps, and reducing the interference of external factors on cell imaging results. It has a high degree of automation and is time-saving and efficient. The cell imaging device 4 provided in this embodiment can realize fully automated detection of multiple cell samples on the same sample 100, and can effectively protect the cell samples, sample 100 and corresponding instruments during cell imaging. The design of the movable shield can realize the anti-misinsertion function, which effectively improves the reliability of the cell imaging device 4 in the fully automated operation process.

[0084] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A cell imaging device, characterized in that, include: frame; A sample mounting device is provided on the frame, and the sample mounting device includes a sample mounting port; A light source device is mounted on the frame and is capable of providing illumination light toward the sample on the sample mounting device; An imaging device is mounted on the frame and located on one side of the sample mounting device; A shielding member is movably mounted on the frame; when the shielding member is in the first position, the sample can be mounted onto the sample mounting device via the sample mounting port. When the shielding member is in the second position, the shielding member blocks the sample mounting path corresponding to the sample mounting port.

2. The cell imaging device according to claim 1, characterized in that, When the shielding member is in the second position, the surface of the shielding member facing the sample mounting port is the first surface; the first surface is provided with a long rib, and when the shielding member is in the first position, the extending direction of the long rib is consistent with the sample mounting path.

3. The cell imaging device according to claim 1, characterized in that, The frame includes a mounting frame having a first opening for the sample to pass through; one end of the shield is pivotally connected to the mounting frame, and the pivot position is located at the top of the first opening; the shield is capable of being raised away from the first opening or lowered towards the first opening.

4. The cell imaging device according to any one of claims 1-3, characterized in that, The sample mounting device includes: A sample clamp, which is movably mounted on the frame; A clamp driving component is disposed on the frame and connected to the sample clamp. The clamp driving component is used to drive the sample clamp to move along the sample mounting path.

5. The cell imaging device according to claim 4, characterized in that, The sample clamp is provided with a mounting space for mounting the sample, and the sample clamp includes a first clamping structure and a second clamping structure; The first clamping structure is located at the edge of the mounting space, and the second clamping structure is located at the top and / or bottom of the mounting space. The clamping direction of the first clamping structure and the clamping direction of the second clamping structure are perpendicular to each other, both facing the center of the mounting space, and both perpendicular to the sample mounting path.

6. The cell imaging device according to claim 4, characterized in that, The sample fixture has a mounting space for mounting the sample, and the sample mounting port is located at one end of the mounting space; the sample fixture includes a sample positioning sensor, which is located at the other end of the mounting space.

7. The cell imaging device according to claim 4, characterized in that, The sample fixture is provided with multiple observation through holes, which are arranged along the straight line of the sample installation path.

8. The cell imaging device according to claim 4, characterized in that, The sample mounting device also includes a position sensor, which is mounted on the frame and is used to detect whether the sample fixture is in a limit movement position.

9. The cell imaging device according to any one of claims 1-3, characterized in that, The imaging device includes: Objective lens, which is movably mounted on the frame; An objective lens drive motor is mounted on the frame and is connected to the objective lens for driving the objective lens to move along a straight line perpendicular to the sample mounting path.

10. A workstation, characterized in that, The workstation includes: case; The cell imaging device according to any one of claims 1-9, wherein the cell imaging device is disposed within the housing; A robotic arm, housed within a housing, is used to grasp the sample and load it onto the sample mounting device via the sample mounting port.

Images