A reaction device of an immobilized enzyme carrier for facilitating timely control of reaction progress

Abstract

The utility model discloses a kind of immobilized enzyme carrier's reaction device of being convenient for and in time control reaction process, belong to the field such as biological chemical industry, food production etc..The device includes magnetic separation unit, fluid circulation system, sensor system, recovery storage tank and automation control box.Magnetic separation unit forms gradient magnetic field by electromagnet array, for adsorbing magnetic immobilized enzyme carrier;Sensor system real-time monitoring enzymatic solution parameter, in combination with automation control box to realize the accurate control of reaction end point;Fluid circulation system controls the directional flow of enzymatic solution;Recovery storage tank is used to store the immobilized enzyme of recovery.The utility model can accurately control reaction process, efficiently recover immobilized enzyme, and have good industrial adaptability, effectively improve production efficiency and product quality.

Description

Technical Field

[0001] This utility model relates to the fields of biochemical engineering and food production technology, specifically a reaction device for immobilized enzyme carriers that facilitates timely control of the reaction process. Background Technology

[0002] Enzymatic hydrolysis is a common reaction method in biochemical and food production processes. The typical method involves adding enzymes to a ground slurry, and after a certain reaction time, passing the hydrolysate through a high-temperature heat exchanger to deactivate the enzymes and prevent over-hydrolysis, which could lead to unintended product quality. However, sometimes the hydrolysate contains starch or large molecules, which can easily gelatinize during the high-temperature plate heat exchanger process. This increases the overall viscosity of the hydrolysate, slows its flow rate in the pipes, and extends the heat exchange time beyond the expected time, potentially causing pipe clogging and affecting the final product quality. It also hinders subsequent separation and other processes. Furthermore, some hydrolysates are not heat-resistant, and high temperatures can damage certain proteins and polysaccharides, affecting the quality of the final product.

[0003] To address the aforementioned problems, immobilized enzyme reaction devices have been developed. Using immobilized enzyme carriers can improve enzyme stability and reusability, and reduce production costs. However, existing immobilized enzyme reaction devices have the following drawbacks:

[0004] The reaction process is difficult to control: the reaction process cannot be effectively monitored, leading to uncontrollable enzymatic hydrolysis and affecting the final reaction product.

[0005] Immobilized enzyme carriers are easily damaged: Ordinary immobilized enzyme carriers are prone to rupture during enzyme hydrolysis, causing enzyme leakage and leading to uncontrollable reactions.

[0006] Low recovery efficiency and significant loss of enzyme activity: When recovering conventional immobilized enzyme carriers, enzyme activity is easily lost during the recovery process.

[0007] Therefore, this application provides a reaction apparatus for immobilized enzyme carriers that facilitates timely control of the reaction process to solve the technical problems existing in the prior art. Utility Model Content

[0008] The purpose of this utility model is to overcome the problems of difficulty in monitoring the reaction endpoint, low recovery efficiency of immobilized enzyme carriers, and easy loss of enzyme activity in the existing technology, and to provide a reaction device with immobilized enzyme carriers that facilitates timely control of the reaction process.

[0009] To achieve the above objectives, this utility model provides the following technical solution:

[0010] A reaction apparatus for immobilized enzyme carriers that facilitates timely control of the reaction process includes:

[0011] Magnetic separation unit: includes an electromagnet array and a separation channel. The electromagnet array is spirally arranged along the outer wall of the separation channel to form a gradient magnetic field, which is used to adsorb magnetically immobilized enzyme carriers.

[0012] Fluid circulation system: including corrosion-resistant circulation pumps, filters and valves, used to control the directional flow of the enzymatic hydrolysate;

[0013] Sensor system: used to monitor various parameters of the enzymatic hydrolysate in real time, including pH, temperature, substrate concentration, turbidity, etc.

[0014] Recovery storage tank: Used to store recovered immobilized enzymes, equipped with a stirring device and a nitrogen protection device;

[0015] Automated control box: It adopts a PLC / HMI integrated system to realize automated control of the separation process, magnetic field switch and reaction endpoint.

[0016] Furthermore, the electromagnet array employs multiple sets of ring electromagnets, spirally arranged along the outer wall of the separation pipe to form a gradient magnetic field, the magnetic field strength of which is adjustable within the range of 0.5-1.5T.

[0017] Furthermore, the separation pipe is made of 316L stainless steel or polytetrafluoroethylene (PTFE) and the inner wall is polished to prevent magnetic particles from adhering to the wall.

[0018] Furthermore, the separation pipe has a diameter of 200mm, a length of 2m, and is installed at an inclination of 10°. It is equipped with a low-shear baffle inside to prolong the magnetic adsorption time and enhance the retention effect of magnetic particles.

[0019] Furthermore, the fluid circulation system adjusts the flow rate of the enzymatic hydrolysate to 0.5-1.0 m / s via a peristaltic pump.

[0020] Furthermore, the sensor system includes devices such as online pH meters, conductivity meters, or near-infrared spectroscopy (NIR) for monitoring changes in substrate concentration.

[0021] Furthermore, the recycling tank is equipped with a stirring device that stirs at a speed of 50 rpm to prevent the magnetically immobilized enzyme from settling, while nitrogen gas is introduced to protect the enzyme activity.

[0022] Furthermore, the automated control box adopts a PLC / HMI integrated system. When the sensor detects that the substrate concentration has dropped to a threshold (e.g., 10% of the initial value), a separation signal is triggered. The PLC controls the corrosion-resistant circulating pump to start, pumping the enzymatic hydrolysate from the bottom of the tank into the electromagnetic separation tube. At the same time, the electromagnet is turned on to adsorb magnetic particles with a magnetic field strength of 1.0T. The separated clear liquid can be returned to the enzymatic hydrolysate tank or discharged into the waste liquid pool. Subsequently, the magnetic field is turned off, and 0.1M PBS buffer solution with pH 7.0 is injected to rinse the pipeline, allowing the magnetic particles to enter the recovery storage tank with the rinsing liquid. The magnetically immobilized enzyme in the recovery storage tank completes the enzyme regeneration process under stirring and nitrogen protection.

[0023] Furthermore, the device also includes an anti-clogging design, a backflushing system that periodically backflushes the pipes, and a low-frequency vibrator (10-20Hz) installed outside the separation pipes to prevent particle deposition and agglomeration.

[0024] Furthermore, the electromagnet uses an intermittent power supply method, and is energized only during the adsorption phase.

[0025] Furthermore, the separation pipe and electromagnet use quick-connect flanges for easy cleaning and replacement.

[0026] Furthermore, the device can perform multi-stage series separation of high-concentration enzymatic hydrolysates, and can also be integrated with a factory CIP (clean-in-place) system through a reserved interface to achieve automated cleaning.

[0027] Compared with the prior art, the beneficial effects of this utility model are:

[0028] 1. Precise control of the reaction process: By monitoring the parameters of the enzymatic hydrolysate in real time through a sensor system and combined with an automated control box, the reaction endpoint can be accurately determined, avoiding over-enzymatic hydrolysis and improving product quality and production efficiency.

[0029] 2. High-efficiency recovery of immobilized enzymes: The gradient magnetic field and inclined pipe design of the magnetic separation unit, as well as reasonable flow rate control, enable the recovery efficiency of magnetically immobilized enzymes to be ≥95%, and the enzyme activity retention rate to be ≥90% during the recovery process, effectively reducing production costs.

[0030] 3. Good industrial adaptability: The device adopts a modular design, which is anti-clogging and low-maintenance. It is easy to integrate with existing enzymatic hydrolysis tanks, enabling automated production. It also supports multi-stage series separation and integration with CIP systems to meet different production scales and needs. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0032] Figure 2 This is a cross-sectional view of the present invention.

[0033] In the diagram: 1. Electromagnet array; 2. Separation pipe; 3. Corrosion-resistant circulating pump; 4. Filter screen; 7. Sensor system; 8. Recycling tank; 9. Automated control box; 10. Baffle plate. Detailed Implementation

[0034] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0035] Example 1:

[0036] This embodiment describes a reaction apparatus with an immobilized enzyme carrier for starch hydrolysis.

[0037] Magnetic separation unit:

[0038] Electromagnet array 1: Ten sets of ring electromagnets are spirally arranged along the outer wall of the separation pipe 2 to form a gradient magnetic field. The magnetic field strength is adjustable; in this embodiment, it is set to 1.0T. The electromagnet coils consist of copper wire and an iron core, encased in a corrosion-resistant 304 stainless steel shell.

[0039] Separation pipe 2: Made of 316L stainless steel with polished inner wall to prevent magnetic particles from adhering to the wall. Separation pipe 2 has a diameter of 200mm, a length of 2m, and is installed at a 10° angle. It is equipped with low-shear force baffles 10 inside. The baffles 10 are made of polytetrafluoroethylene (PTFE), with a thickness of 5mm and a spacing of 200mm.

[0040] Fluid circulation system:

[0041] Corrosion-resistant circulating pump 3: It adopts a peristaltic pump with model number BT300-2J, with a flow range of 0.1-300ml / min, and is made of polypropylene.

[0042] Filter 4: Removably mounted on the pump body.

[0043] Valves: Manual ball valves are used to control the flow direction of the enzymatic hydrolysate.

[0044] Enzyme hydrolysate flow rate: The flow rate of the enzyme hydrolysate is adjusted to 0.8 m / s using a peristaltic pump.

[0045] Sensor System 7:

[0046] pH meter: Mettler Toledo InLab Routine Pro pH meter, with a measurement range of 0-14 pH and an accuracy of ±0.01 pH.

[0047] Conductivity meter: Mettler Toledo SevenEasy Cond conductivity meter is used, with a measurement range of 0-2000 mS / cm and an accuracy of ±0.5%.

[0048] Turbidimeter: Hach 2100Q portable turbidity meter, with a measurement range of 0-1000 NTU and an accuracy of ±2%.

[0049] The instruments used in the sensor system are all existing technologies, so their principles will not be elaborated further.

[0050] Recycling storage tank 8:

[0051] Tank material: Made of 304 stainless steel, with a volume of 50L.

[0052] Stirring device: A top-mounted stirrer with adjustable speed is used; in this embodiment, it is set to 50 rpm. The stirring paddle is made of 316L stainless steel.

[0053] Nitrogen protection device: connected to the top of the storage tank via a nitrogen cylinder, with a nitrogen flow rate of 1L / min.

[0054] Automation control box 9:

[0055] PLC: Siemens S7-1200 PLC is used.

[0056] HMI: Adopts Siemens KTP700 Basic HMI.

[0057] Control program: Write a PLC control program to achieve automated control of the separation process, magnetic field switch and reaction endpoint. The control program is a common control system in existing technology, and the specific program content will not be described in detail.

[0058] Operating steps:

[0059] 1. Add the magnetically immobilized amylase to the enzymatic hydrolysis tank, add starch slurry, adjust the pH to 6.0, and set the temperature to 50℃.

[0060] 2. Turn on the stirrer to thoroughly mix the enzyme and substrate.

[0061] 3. The pH, temperature, substrate concentration, and turbidity of the enzymatic hydrolysate are monitored in real time by the sensor system 7.

[0062] 4. When the sensor detects that the substrate concentration has dropped to 10% of the initial value, a separation signal is triggered. The PLC controls the corrosion-resistant circulating pump 3 to start, pumping the enzymatic hydrolysate from the bottom of the tank into the electromagnetic separation tube. At the same time, the electromagnet is turned on to adsorb magnetic particles with a magnetic field strength of 1.0T.

[0063] 5. The separated supernatant is returned to the enzymatic hydrolysis tank.

[0064] 6. After separation is complete, turn off the magnetic field and inject 0.1M PBS buffer (pH 7.0) to flush the pipeline, allowing the magnetic particles to enter the recovery tank 8 along with the flushing solution.

[0065] 7. The magnetically immobilized enzyme in the recovery tank 8 completes the enzyme regeneration process under stirring and nitrogen protection.

[0066] Experimental results:

[0067] In this embodiment, the recovery efficiency of magnetically immobilized amylase was 96%, and the enzyme activity retention rate was 92%.

[0068] Example 2:

[0069] This embodiment describes a reaction apparatus with an immobilized enzyme carrier for proteolytic reactions, which differs from Embodiment 1 in the following main aspects:

[0070] Type of enzyme: Immobilized protease is used.

[0071] Substrate: Soy protein slurry.

[0072] pH and temperature: Adjust pH to 8.0 and temperature to 40°C.

[0073] Sensor: An online amino acid analyzer is used to monitor changes in amino acid concentration.

[0074] Washing solution: Use 0.1M Tris-HCl buffer at pH 8.0.

[0075] Operating steps:

[0076] 1. Add the magnetically immobilized protease to the enzymatic hydrolysis tank, add soybean protein slurry, adjust the pH to 8.0, and the temperature to 40℃.

[0077] 2. Turn on the stirrer to thoroughly mix the enzyme and substrate.

[0078] 3. The pH, temperature and amino acid concentration of the enzymatic hydrolysate are monitored in real time by the sensor system 7.

[0079] 4. When the sensor detects that the amino acid concentration has reached the preset value, a separation signal is triggered. The PLC controls the corrosion-resistant circulating pump 3 to start, pumping the enzymatic hydrolysate from the bottom of the tank into the electromagnetic separation tube. At the same time, the electromagnet is turned on to adsorb magnetic particles with a magnetic field strength of 1.0T.

[0080] 5. The separated clear liquid is discharged into the waste liquid pool.

[0081] 6. After separation is complete, turn off the magnetic field and flush the pipeline with 0.1M Tris-HCl buffer solution at pH 8.0, so that the magnetic particles enter the recovery tank 8 with the flushing solution.

[0082] 7. The magnetically immobilized enzyme in the recovery tank 8 completes the enzyme regeneration process under stirring and nitrogen protection.

[0083] Experimental results:

[0084] In this embodiment, the recovery efficiency of the magnetically immobilized protease was 95%, and the enzyme activity retention rate was 90%.

[0085] The workflow of the control program in Embodiments 1 and 2 of this application is as follows: When the sensor detects that the substrate concentration has dropped to a threshold, such as 10% of the initial value, a separation signal is triggered. The PLC controls the corrosion-resistant circulating pump to start, pumping the enzymatic hydrolysate from the bottom of the tank into the electromagnetic separation tube. At the same time, the electromagnet is turned on to adsorb magnetic particles with a magnetic field strength of 1.0T. The separated clear liquid can be returned to the enzymatic hydrolysate tank or discharged into the waste liquid pool. Then the magnetic field is turned off, and 0.1M PBS buffer solution with pH 7.0 is injected to rinse the pipeline, so that the magnetic particles enter the recovery storage tank with the rinsing liquid. The magnetically immobilized enzyme in the recovery storage tank completes the enzyme regeneration process under stirring and nitrogen protection. The entire process does not involve any improvement in computer algorithms and adopts the existing control system algorithm.

[0086] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A reaction apparatus for immobilized enzyme carriers that facilitates timely control of the reaction process, characterized in that, include: The magnetic separation unit includes an electromagnet array and a separation channel. The electromagnet array is spirally arranged along the outer wall of the separation channel to form a gradient magnetic field for adsorbing magnetically immobilized enzyme carriers. A fluid circulation system, including a corrosion-resistant circulation pump, filters, and valves, is used to control the directional flow of the enzymatic hydrolysate; A sensor system is used to monitor various parameters of the enzymatic hydrolysate in real time, including pH, temperature, substrate concentration, and turbidity. A recycling storage tank, used to store the recovered immobilized enzymes, is equipped with a stirring device and a nitrogen protection device; The automated control box adopts a PLC / HMI integrated system to realize automated control of the separation process, magnetic field switch and reaction endpoint.

2. The reaction apparatus according to claim 1, characterized in that, The electromagnet array uses multiple sets of ring electromagnets, spirally arranged along the outer wall of the separation pipe to form a gradient magnetic field, the magnetic field strength of which is adjustable in the range of 0.5-1.5T.

3. The reaction apparatus according to claim 1, characterized in that, The separation pipe is made of 316L stainless steel or polytetrafluoroethylene.

4. The reaction apparatus according to claim 1, characterized in that, The separation pipe has a diameter of 200 mm, a length of 2 m, is installed at an angle of 10°, and is equipped with a low shear force baffle inside.

5. The reaction apparatus according to claim 1, characterized in that, The fluid circulation system uses a peristaltic pump to regulate the flow rate of the enzymatic hydrolysate between 0.5 and 1.0 m / s.

6. The reaction apparatus according to claim 1, characterized in that, The sensor system includes an online pH meter, conductivity meter, or near-infrared spectroscopy device for monitoring changes in substrate concentration.

7. The reaction apparatus according to claim 1, characterized in that, The recycling tank is equipped with a stirring device that stirs at 50 rpm to prevent the magnetically immobilized enzyme from settling, while nitrogen gas is introduced to protect the enzyme activity.

8. The reaction apparatus according to claim 1, characterized in that, The automated control box adopts a PLC / HMI integrated system. When the sensor detects that the substrate concentration has dropped to a threshold, such as 10% of the initial value, a separation signal is triggered. The PLC controls the corrosion-resistant circulation pump to start, pumping the enzymatic hydrolysate from the bottom of the tank into the electromagnetic separation tube. At the same time, the electromagnet is turned on to adsorb magnetic particles with a magnetic field strength of 1.0T. The separated clear liquid can be returned to the enzymatic hydrolysate tank or discharged into the waste liquid pool. Then the magnetic field is turned off, and 0.1M PBS buffer solution with pH 7.0 is injected to rinse the pipeline, so that the magnetic particles enter the recovery storage tank with the rinsing liquid. The magnetically immobilized enzyme in the recovery storage tank completes the enzyme regeneration process under stirring and nitrogen protection.

9. The reaction apparatus according to claim 1, characterized in that, It also includes anti-clogging design, setting up a backflushing system to periodically backflush the pipes, and installing a low-frequency vibrator outside the separate pipes.

10. The reaction apparatus according to claim 1, characterized in that, The electromagnet is powered intermittently, only energized during the adsorption phase.

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