Visualized culture device for bovine and ovine embryos

Abstract

The application discloses a visualized cattle and sheep embryo culture device and belongs to the technical field of cattle and sheep embryo in-vitro culture, which comprises an outer frame, the inside of the outer frame is provided with a culture layer, the inside of the culture layer is provided with a culture rack, the culture layer comprises a layered frame, the front end of the layered frame is embedded with a glass window, the bottom of the glass window is provided with a handle, and pull-out grooves are formed in the outer walls of the two ends of the layered frame. The device is characterized in that the layered plate sent by the alignment of the adjusting frame and the inclined mirror plate carried in each layered frame is aligned, the inclined angle of the inclined mirror plate is aligned with the glass window after being illuminated by the LED illuminating lamp, the user can conveniently observe the picture of the culture tank reflected on the inclined mirror plate through the glass window, the adjusting frame can be slid by rotating the threaded rod during the process, the observation position can be adjusted, and then the internal observation can be carried out without taking out the layered frame as a whole, so that the device is convenient to take and use and is more beneficial to cultivation observation.

Description

Technical Field

[0001] This application relates to the field of in vitro culture technology for bovine and ovine embryos, specifically to a visualized bovine and ovine embryo culture device. Background Technology

[0002] The embryos of cattle and sheep are immature fetal tissues that form in the early stages of pregnancy, usually in the first 3-4 months. They consist of a group of cells that have developed from the fertilized egg into the early stage of development in the uterus. At this time, the embryo has not yet fully differentiated into organs, but it already has life activity. The embryo is in a highly active stage and contains a large number of growth factors and immunoglobulins. It is an early product of the development of the cow or sheep egg after fertilization. It is mainly composed of embryonic cells and unabsorbed nutrient solution. When culturing sheep embryos in vitro, an incubator is required. The in vitro incubator for cattle and sheep embryos is a key piece of equipment used to support the in vitro development of embryos. Its core technology lies in simulating the maternal uterine environment and optimizing culture conditions.

[0003] Existing technology, such as patent publication number CN221544650U, discloses an in vitro culture box for Hu sheep embryos, including a culture box body. A fixed guide rail is fixedly connected to the inner wall of the culture box body. A fixed block is fixedly connected to the inner wall of the fixed guide rail. A receiving groove is opened inside the fixed block. A telescopic spring is fixedly connected to the inner wall of the receiving groove. A slider is fixedly connected to one end of the telescopic spring. A rolling element is movably connected to the outer wall of the slider. A support plate is arranged inside the fixed guide rail. A positioning block is fixedly connected to the top of the support plate. A sealing plate is fixedly connected to the front end of the support plate. A temperature sensor is fixedly connected to the top inner wall of the culture box body.

[0004] In practical applications, the aforementioned sheep embryo in vitro development device mainly improves the convenience of removal and storage through the pull-out structure, solving the problem of cumbersome operation. However, in the actual cultivation process, due to the different embryo samples, it is necessary to observe them in stages. During cultivation, it is not possible to pull them out for observation at will, which is very inconvenient. Therefore, we propose a visual bovine and sheep embryo culture device. Summary of the Invention

[0005] The summary section of this application is intended to provide a brief overview of the concepts, which will be described in detail in the detailed description section below. This summary section is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to limit the scope of the claimed technical solutions.

[0006] The purpose of this application is to provide a visualized bovine and ovine embryo culture device to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this application provides the following technical solution: a visual bovine and ovine embryo culture device, comprising an outer frame, a culture layer disposed inside the outer frame, a culture rack disposed inside the culture layer, the culture layer comprising a layered frame, a glass window embedded at the front end of the layered frame, a handle disposed at the bottom of the glass window, pull-out grooves formed on the outer walls at both ends of the layered frame, an adjustment frame disposed at the top end of the layered frame, adjustment grooves fitted at both ends of the adjustment frame, a threaded rod passing through the center of the adjustment frame, an adjustment knob connected to one end of the threaded rod, an LED light embedded on one side of the bottom end of the adjustment frame, and a tilting mirror plate fixed on the other side of the bottom end of the adjustment frame.

[0008] Furthermore, the tilting mirror plate and the adjustment frame form a threaded transmission structure with the layered frame via a threaded rod, and the tilting angle of the tilting mirror plate is horizontally aligned with the height dimension of the glass window.

[0009] Furthermore, the glass window and handle are fixedly connected to the layered frame, and the layered frame is slidably pulled out and engaged with the front end of the outer frame through pull-out slots on both sides.

[0010] Furthermore, the outer frame includes an outer casing, and a temperature monitoring panel is provided on one side of the top surface of the outer casing. An air conditioning temperature control component is provided inside the bottom end of the outer casing. A front sealing plate is engaged with the front end of the outer casing, and bolts are distributed around the front sealing plate.

[0011] Furthermore, the interior of the outer casing is connected to the air conditioning temperature control component, and the front cover plate is bolted to the front end of the outer casing to form a detachable structure.

[0012] Furthermore, the cultivation rack includes a layered plate, the four sides of which are fixed to the inner wall of the bottom end of the layered frame, a cultivation trough is fixed on the top surface of the layered plate, and through holes are opened inside the layered plate.

[0013] Furthermore, the culture tanks are arranged equidistantly along the top surface of the layered plate, and the through holes are distributed and penetrate along the gaps between the culture tanks.

[0014] Compared with the prior art, the beneficial effects of this application are:

[0015] This sheep embryo in vitro development device uses an adjustment frame and tilting mirror plate mounted in each layer to align the lower layered plates. After being illuminated by LED lights, the tilting angle of the tilting mirror plate is aligned with the glass window, allowing users to easily observe the culture tank image reflected on the tilting mirror plate through the glass window. During the process, the adjustment frame can be slid by rotating the threaded rod to adjust the observation position, so that internal observation can be carried out without removing the entire layered frame. This makes it convenient to use and more conducive to cultivation and observation.

[0016] This sheep embryo in vitro development device uses an outer box as the main culture space. The internal temperature changes can be monitored through a temperature monitoring panel fixed on one side of the outer box, which can be used in conjunction with the air conditioning temperature control components to regulate the internal temperature and ensure that the culture temperature meets the requirements. At the same time, the outer box provides a stable culture space for the embryos in the internal culture rack, and the front cover is removable for easy internal cleaning and maintenance.

[0017] This sheep embryo in vitro development device can place culture dishes in the culture tank on the top surface of the layered plate. It can be used with the pull-out structure of the layered frame to quickly place the layered plate into the device for culture. Each layered plate has through holes inside to facilitate uniform internal temperature flow and maintain a constant and stable culture temperature. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of the outer frame of this application;

[0019] Figure 2 This is a schematic diagram of the three-dimensional structure of the culture layer in this application;

[0020] Figure 3 This is a side view of the internal structure of the culture layer in this application;

[0021] Figure 4 This is a schematic diagram of the three-dimensional structure of the cultivation rack in this application.

[0022] In the diagram: 1. Outer frame; 101. Outer casing; 102. Temperature monitoring panel; 103. Air conditioning temperature control component; 104. Front sealing plate; 105. Bolt; 2. Culture layer; 201. Layering frame; 202. Glass window; 203. Handle; 204. Pull-out slot; 205. Adjustment rack; 206. Adjustment groove; 207. Threaded rod; 208. Adjustment knob; 209. LED lighting; 210. Inclined mirror plate; 3. Culture rack; 301. Layering plate; 302. Culture tank; 303. Through hole. Detailed Implementation

[0023] Embodiments of this disclosure will now be described in more detail with reference to the accompanying drawings. While some embodiments of this disclosure are shown in the drawings, it should be understood that this disclosure can be implemented in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of this disclosure. It should be understood that the accompanying drawings and embodiments of this disclosure are for illustrative purposes only and are not intended to limit the scope of protection of this disclosure.

[0024] It should also be noted that, for ease of description, only the parts relevant to the invention are shown in the accompanying drawings. Unless otherwise specified, the embodiments and features described in this disclosure can be combined with each other.

[0025] It should be noted that the concepts of "first" and "second" mentioned in this disclosure are used only to distinguish different devices, modules, or units, and are not used to limit the order of the functions performed by these devices, modules, or units or their interdependencies.

[0026] It should be noted that the terms "a" and "a plurality of" used in this disclosure are illustrative rather than restrictive, and those skilled in the art should understand that, unless otherwise expressly indicated in the context, they should be understood as "one or more".

[0027] This disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0028] This application provides, as follows: Figure 1-4 The illustrated visualization device for culturing bovine and ovine embryos includes an outer frame 1, a culture layer 2 inside the outer frame 1, a culture rack 3 inside the culture layer 2, an outer box 101, a temperature monitoring panel 102 on one side of the top surface of the outer box 101, an air conditioning temperature control component 103 inside the bottom end of the outer box 101, a front sealing plate 104 engaged at the front end of the outer box 101, and bolts 105 distributed around the front sealing plate 104.

[0029] To provide external protection and stability for the overall culture device during use, such as Figure 1 As shown, this sheep embryo in vitro development device uses the outer box 101 as the main culture space. The internal temperature changes can be monitored through the temperature monitoring panel 102 fixed on one side of the outer box 101, which can be used in conjunction with the air conditioning temperature control component 103 to regulate the internal temperature and ensure that the culture temperature meets the requirements. At the same time, the outer box 101 provides a stable culture space for the embryos in the internal culture rack 3. Meanwhile, the front cover 104 is removable for easy internal cleaning and maintenance.

[0030] like Figure 2-3 As shown, the culture layer 2 includes a layered frame 201. A glass window 202 is embedded in the front end of the layered frame 201. A handle 203 is provided at the bottom of the glass window 202. Pull-out grooves 204 are provided on the outer walls of both ends of the layered frame 201. An adjustment frame 205 is provided at the top of the layered frame 201. Adjustment grooves 206 are attached to both ends of the adjustment frame 205. A threaded rod 207 is passed through the center of the adjustment frame 205. An adjustment knob 208 is connected to one end of the threaded rod 207. An LED light 209 is embedded in one side of the bottom end of the adjustment frame 205. An inclined mirror plate 210 is fixed to the other side of the bottom end of the adjustment frame 205.

[0031] To maintain visibility during the cultivation process, such as Figure 2-3As shown, this sheep embryo in vitro development device can align the lowered layered plates 301 with the adjustment frame 205 and tilting mirror plate 210 mounted in each layered frame 201. After being illuminated by LED lights 209, the tilt angle of the tilting mirror plate 210 is aligned with the glass window 202, allowing the user to observe the culture tank 302 reflected on the tilting mirror plate 210 through the glass window 202. During the process, the adjustment frame 205 can be slid by rotating the threaded rod 207 to adjust the observation position, thus allowing for internal observation without having to pull out the entire layered frame 201. This makes it convenient to use and facilitates cultivation and observation.

[0032] like Figure 4 As shown, the cultivation rack 3 includes a layered plate 301. The four sides of the layered plate 301 are fixed to the inner wall of the bottom end of the layered frame 201. A cultivation trough 302 is fixed on the top surface of the layered plate 301. A through hole 303 is opened inside the layered plate 301.

[0033] Finally, in order to provide a stable environment for embryo culture, such as Figure 4 As shown, this sheep embryo in vitro development device can place culture dishes through the culture tank 302 placed on the top surface of the layered plate 301. It can be used with the pull-out structure of the layered frame 201 to quickly place the layered plate 301 into the device for culture. Each layered plate 301 has through holes 303 distributed inside, which can facilitate uniform internal temperature flow and maintain a constant and stable culture temperature.

[0034] In summary, when using this sheep embryo in vitro development device, the user first places the culture dish containing the bovine or sheep embryos into the culture tank 302 on the top surface of the layered plate 301. Then, the layered frame 201, which is fixed to the layered plate 301, is slid into the outer casing 101 through the pull-out slots 204 on both sides. The air conditioning temperature control component 103 is turned on for constant temperature cultivation, and the temperature is controlled by the temperature monitoring panel 102. When it is necessary to observe each culture tank 302, the user can rotate the adjustment knob 208. The threaded rod 207 connected to the adjustment knob 208 drives the adjustment frame 205 to slide along the adjustment slots 206 on both sides to adjust its position. When the adjustment frame 205 is above the culture tank 302, the LED lighting 209 is turned on for supplemental lighting. Then, the image of the culture dish in the culture tank 302 is reflected by the tilted mirror plate 210 at an inclined angle. The user can directly observe through the glass window 202, which is horizontally aligned with the inclined surface of the mirror, thus completing the observation during the cultivation process.

[0035] The above description is merely a selection of preferred embodiments of this disclosure and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the invention involved in the embodiments of this disclosure is not limited to technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalents without departing from the above-described inventive concept. For example, technical solutions formed by substituting the above-described features with (but not limited to) technical features with similar functions disclosed in the embodiments of this disclosure.

Claims

1. A visual bovine and ovine embryo culture device, comprising an outer frame (1), characterized in that, The outer frame (1) is provided with a culture layer (2) inside, and a culture rack (3) is provided inside the culture layer (2). The culture layer (2) includes a layered frame (201). A glass window (202) is embedded in the front end of the layered frame (201). A handle (203) is provided at the bottom of the glass window (202). Pull-out grooves (204) are provided on the outer walls of both ends of the layered frame (201). An adjustment frame (205) is provided at the top of the layered frame (201). An adjustment groove (206) is attached to both ends of the adjustment frame (205). A threaded rod (207) is passed through the center of the adjustment frame (205). An adjustment knob (208) is connected to one end of the threaded rod (207). An LED light (209) is embedded on one side of the bottom end of the adjustment frame (205). An inclined mirror plate (210) is fixed on the other side of the bottom end of the adjustment frame (205).

2. The visualized bovine and ovine embryo culture device according to claim 1, characterized in that, The tilted mirror plate (210) and the adjustment bracket (205) form a threaded transmission structure with the layered frame (201) through the threaded rod (207), and the tilt angle of the tilted mirror plate (210) is horizontally aligned with the height dimension of the glass window (202).

3. The visualized bovine and ovine embryo culture device according to claim 1, characterized in that, The glass window (202) and handle (203) are fixedly connected to the layered frame (201), and the layered frame (201) is slidably pulled out and engaged with the front end of the outer frame (1) through the pull-out grooves (204) on both sides.

4. The visualized bovine and ovine embryo culture device according to claim 1, characterized in that, The outer frame (1) includes an outer box (101), and a temperature monitoring panel (102) is provided on one side of the top surface of the outer box (101). An air conditioning temperature control component (103) is provided inside the bottom end of the outer box (101). A front sealing plate (104) is engaged at the front end of the outer box (101), and bolts (105) are distributed around the front sealing plate (104).

5. A visualized bovine and ovine embryo culture device according to claim 4, characterized in that, The interior of the outer casing (101) is connected to the air conditioning temperature control component (103), and the front cover plate (104) is connected to the front end of the outer casing (101) by bolts (105) to form a detachable structure.

6. The visualized bovine and ovine embryo culture device according to claim 1, characterized in that, The cultivation rack (3) includes a layered plate (301), the four sides of which are fixed to the inner wall of the bottom end of the layered frame (201), the top surface of the layered plate (301) is fixed with a cultivation trough (302), and the interior of the layered plate (301) is provided with a through hole (303).

7. A visualized bovine and ovine embryo culture device according to claim 6, characterized in that, The culture tanks (302) are arranged equidistantly along the top surface of the layered plate (301), and the through holes (303) are distributed and penetrate along the gaps between the culture tanks (302).

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