Plant tissue culture seedling carbon dioxide supplementing device

Abstract

The utility model provides a kind of plant tissue culture seedling carbon dioxide supplement device, including storage bottle, its inside forms gas storage cavity;And the storage bottle bottom is equipped with air inlet passage and gas outlet passage;Supplement component, it includes fixed disc, rubber ring, screw rod, handle, push plate, support rod and top plate;Wherein, the fixed disc is placed in storage bottle interior, the rubber ring is fixedly connected with fixed disc outside, the rubber ring is formed dynamic seal with the storage bottle inner wall;And anti-reflux mechanism, the anti-reflux mechanism is equipped in storage bottle, for realizing the one-way flow of gas in the process of being filled with air or being exhausted from air inlet passage or gas outlet passage.This device when using, make in to the carbon dioxide of storage tank interior flush, carbon dioxide can maintain set amount, not make the carbon dioxide amount that flushes into storage bottle appear difference, so that the carbon dioxide amount that supplement in tissue culture box is identical.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, specifically to a carbon dioxide supplementation device for plant tissue culture seedlings. Background Technology

[0002] Plant tissue culture (TTC) is a technique that utilizes the totipotency of plant cells under sterile conditions to rapidly propagate superior varieties through in vitro culture. TTC seedlings offer advantages such as rapid propagation, virus elimination, and preservation of genetic stability, and are widely used in breeding, conservation of rare plants, and commercial seedling production. During plant tissue culture, appropriate amounts of carbon dioxide are added to optimize photosynthesis, promote plant growth, and improve the quality of the tissue-cultured seedlings.

[0003] However, currently, when adding carbon dioxide, the tissue culture box and the carbon dioxide bottle are usually connected directly by a pipe, and the carbon dioxide is transported into the tissue culture box through the pipe. However, due to the difference in the transmission speed during the internal transmission of carbon dioxide, the amount of carbon dioxide added to the tissue culture box is different, which affects the quality of the plant tissue culture seedlings. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a carbon dioxide supplementation device for plant tissue culture seedlings.

[0005] The specific technical solution is as follows:

[0006] A carbon dioxide supplementation device for plant tissue culture seedlings, comprising:

[0007] The storage bottle has an internal gas storage cavity; and the bottom of the storage bottle is provided with an air inlet channel and an air outlet channel.

[0008] The supplementary components include a fixed disc, a rubber ring, a screw, a handle, a push plate, a support rod, and a top plate. The fixed disc is placed inside the storage bottle, the rubber ring is fixedly connected to the outer side of the fixed disc, and the rubber ring forms a dynamic seal with the inner wall of the storage bottle. The screw vertically penetrates the storage bottle and is threadedly connected to a threaded groove at the center of the top of the storage bottle. The bottom end of the screw is rotatably connected to the fixed disc. The handle is fixedly connected to the top of the screw. The push plate is slidably connected to the lower end of the storage bottle. The support rod vertically penetrates the storage bottle and is fixedly connected to the top of the push plate. The top plate is fixedly connected to the top of the support rod. When the screw rotates, it drives the fixed disc to move axially along the storage bottle to compress the storage cavity volume, thereby limiting the initial storage amount of carbon dioxide. The push plate achieves quantitative emission through gas pressure displacement.

[0009] An anti-backflow mechanism is provided inside the storage bottle to enable unidirectional gas flow during the process of filling the air inlet channel or exhausting the air from the outlet channel.

[0010] Optionally, the anti-backflow mechanism includes a fixed rod, a support plate, a sealing plug, a vent, a base plate, and a support spring. The support plate is movably disposed inside the storage bottle. One end of the fixed rod is fixedly connected to the bottom of the support plate. The fixed rod is vertically inserted and slidably connected to a central channel opened at the center of the bottom of the storage bottle. The base plate is installed on the other end of the fixed rod. The support spring is sleeved on the outside of the fixed rod and is located between the bottom of the storage bottle and the base plate. The sealing plug is fixedly connected to the bottom of the support plate. The vent is opened on the side of the bottom of the support plate away from the sealing plug. The vent and the sealing plug correspond to the positions of the air inlet channel and the air outlet channel, respectively. When the sealing plug closes the air outlet channel, the vent communicates with the air inlet channel; otherwise, it closes the air inlet channel and opens the air outlet channel.

[0011] Optionally, the top end of the support spring contacts the bottom end of the storage bottle, and the top end of the support spring contacts the top end of the base plate.

[0012] Optionally, an air intake pipe is connected to the air intake channel, and an air outlet pipe is connected to the air outlet channel.

[0013] Optionally, the contact portion of the sealing plug with the air outlet or air inlet channel has a conical structure.

[0014] Optionally, the sealing plug is made of an elastic material.

[0015] Optionally, the handle is ergonomically designed with anti-slip textures on its surface to facilitate easy rotation of the screw by the operator.

[0016] Optionally, both the air inlet pipe and the air outlet pipe are made of corrosion-resistant materials.

[0017] Optionally, the storage bottle has graduation lines on its outer surface.

[0018] Optionally, the storage bottle is made of a transparent or translucent material to facilitate observation of the amount of carbon dioxide stored inside the storage bottle and its working status.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] This application provides a carbon dioxide supplementation device for plant tissue culture seedlings. By setting up a supplementation component, rotating the handle causes the screw to rotate. The screw moves upward under the drive of the threaded structure, causing the fixed disc and rubber ring to move upward inside the storage bottle. When the fixed disc moves to the desired position, it can block the push plate, so that when carbon dioxide is injected into the storage box, the carbon dioxide can be maintained at a set amount, and the amount of carbon dioxide injected into the storage bottle will not be different, thus ensuring that the amount of carbon dioxide supplemented in the tissue culture box is the same. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a cross-sectional structural diagram illustrating the internal structure of the storage bottle according to this utility model;

[0023] Figure 3 This is a cross-sectional structural diagram illustrating the supplementary components of this utility model;

[0024] Figure 4 This is a cross-sectional structural diagram illustrating the anti-backflow mechanism of this utility model.

[0025] In the diagram: 11. Storage bottle; 12. Threaded groove; 21. Fixed disc; 22. Rubber ring; 23. Screw; 24. Handle; 25. Push plate; 26. Support rod; 27. Top plate; 31. Fixed rod; 32. Support plate; 33. Sealing plug; 34. Vent hole; 35. Bottom plate; 36. Support spring; 37. Air inlet pipe; 38. Air outlet pipe. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments of the present invention can be combined with each other.

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention.

[0029] This utility model provides a carbon dioxide supplementation device for plant tissue culture seedlings, referring to... Figures 1-4 ,include:

[0030] Storage bottle 11 has a gas storage cavity inside; and the bottom of storage bottle 11 is provided with an air inlet channel and an air outlet channel.

[0031] The supplementary components include a fixed disc 21, a rubber ring 22, a screw 23, a handle 24, a push plate 25, a support rod 26, and a top plate 27. The fixed disc 21 is placed inside the storage bottle 11, and the rubber ring 22 is fixedly connected to the outside of the fixed disc 21, forming a dynamic seal with the inner wall of the storage bottle 11. The screw 23 vertically penetrates the storage bottle 11 and is threadedly connected to a threaded groove 12 at the center of the top of the storage bottle 11. The bottom end of the screw 23 is rotatably connected to the fixed disc 21. The handle 24 is fixedly connected to the top of the screw 23, the push plate 25 is slidably connected to the lower end of the storage bottle 11, the support rod 26 vertically penetrates the storage bottle 11, and the fixed disc 21 is fixedly connected to the top of the push plate 25. The top plate 27 is fixedly connected to the top of the support rod 26. When the screw 23 rotates, it drives the fixed disc 21 to move axially along the storage bottle 11 to compress the volume of the storage chamber, thereby limiting the initial storage amount of carbon dioxide. The push plate 25 achieves quantitative discharge through gas pressure displacement.

[0032] The anti-backflow mechanism is located inside the storage bottle 11 and is used to achieve unidirectional gas flow during the process of filling the air inlet channel or exhausting the air from the outlet channel.

[0033] Specifically, the operator holds the handle 24 and slowly rotates it. Since the handle 24 is fixedly connected to the top of the screw 23, the rotation of the handle 24 will cause the screw 23 to rotate in the threaded groove 12 at the top of the storage bottle 11. Because the bottom end of the screw 23 is rotatably connected to the fixed disc 21, the rotation of the screw 23 will drive the fixed disc 21 to move upward or downward along the axial direction of the storage bottle 11. When the fixed disc 21 moves upward, it will compress the gas storage chamber volume inside the storage bottle 11. According to the scale that may be marked on the storage bottle 11 or according to the preset moving distance, when the fixed disc 21 moves to the appropriate position, the operator stops rotating the handle 24, at which point the initial storage amount of carbon dioxide is limited. Connect the carbon dioxide gas source to the inlet channel of the storage bottle 11, open the valve of the carbon dioxide gas source, and the carbon dioxide gas begins to enter the storage bottle 11 through the inlet channel. As the gas enters, the gas pressure inside the storage bottle 11 gradually increases, and the gas pushes the push plate 25 to move upward. Since the push plate 25 is connected to the support rod 26 and the top plate 27, the push plate 25 will move upward smoothly under the action of gas pressure. When the push plate 25 moves upward and contacts the fixed disk 21, the push plate 25 stops moving due to the limiting effect of the fixed disk 21. At this time, the storage bottle 11 has been filled with the predetermined initial storage amount of carbon dioxide gas. Connect the gas outlet channel of storage bottle 11 to the pipe leading to the tissue culture seedling environment. Pushing the top plate 27, which is connected to the support rod 26 and push plate 25, causes the support rod 26 and push plate 25 to move downwards. As the push plate 25 moves downwards, it discharges the carbon dioxide gas in storage bottle 11 through the gas outlet channel, thus supplementing the tissue culture seedling environment with carbon dioxide. During the venting process, the anti-backflow mechanism ensures that the gas can only be discharged from the gas outlet channel without backflow. In practical applications, this device can stably and reliably achieve the carbon dioxide supplementation function according to design requirements, providing a stable carbon dioxide supply environment for plant tissue culture seedlings, which helps to improve the quality and yield of tissue culture seedlings.

[0034] Reference Figure 4The backflow prevention mechanism includes a fixed rod 31, a support plate 32, a sealing plug 33, a vent 34, a base plate 35, and a support spring 36. The support plate 32 is movably disposed inside the storage bottle 11. One end of the fixed rod 31 is fixedly connected to the bottom of the support plate 32. The fixed rod 31 is vertically inserted and slidably connected to the central channel opened at the center of the bottom of the storage bottle 11. The base plate 35 is installed on the other end of the fixed rod 31. The support spring 36 is sleeved on the outside of the fixed rod 31 and is located between the bottom of the storage bottle 11 and the base plate 35. The sealing plug 33 is fixedly connected to the bottom of the support plate 32. The vent 34 is opened on the side of the bottom of the support plate 32 away from the sealing plug 33. The vent 34 and the sealing plug 33 correspond to the positions of the air inlet channel and the air outlet channel, respectively. When the sealing plug 33 closes the air outlet channel, the vent 34 is connected to the air inlet channel. Conversely, when the sealing plug 33 closes the air inlet channel, the air outlet channel is opened. Before inflating the inlet channel, the vent 34 is connected to the inlet channel, and the sealing plug 33 closes the outlet channel. When inflating the inlet channel, the gas entering the storage bottle 11 exerts upward pressure on the support plate 32. Under the traction of the support spring 36, the sealing plug 33 will continuously close the outlet channel, allowing carbon dioxide gas to smoothly enter the storage bottle 11 through the vent 34 without leaking from the outlet channel. Before venting from the outlet channel, the sealing plug 33 seals the inlet channel. When venting from the outlet channel, the gas pressure inside the storage bottle 11 pushes the support plate 32 downward. During this process, the support plate 32 drives the sealing plug 33 downward, causing the sealing plug 33 to close the inlet channel. Simultaneously, the vent 34 connects to the outlet channel, allowing carbon dioxide gas inside the storage bottle 11 to be discharged through the vent 34 and the outlet channel without flowing back into the inlet channel.

[0035] The top end of the support spring 36 is in contact with the bottom end of the storage bottle 11, and the top end of the support spring 36 is in contact with the top end of the base plate 35. In this embodiment, the two ends of the support spring 36 are set to be in contact rather than fixed, so as to facilitate the rotation of the support plate 32 and realize the position exchange between the sealing plug 33 and the vent hole 34.

[0036] An inlet pipe 37 connects to the inlet channel, and an outlet pipe 38 connects to the outlet channel. The inlet pipe 37 connects the external carbon dioxide source to the inlet channel of the storage bottle 11, providing an input channel for carbon dioxide gas. Because the inlet pipe 37 is made of corrosion-resistant materials (such as stainless steel), it ensures stable gas transmission and prevents gas leakage or flow changes due to pipe corrosion. The outlet pipe 38 delivers the carbon dioxide gas from the storage bottle 11 to the plant tissue culture seedling environment. Its similar corrosion-resistant properties ensure that there will be no malfunctions during gas transmission, stably replenishing the target environment with carbon dioxide.

[0037] The sealing plug 33 can be made of rubber. The contact portion of the sealing plug 33 with the air outlet or inlet channel has a conical structure. When gas applies pressure to the sealing plug 33, a pressure component perpendicular to the channel wall is generated on the side of the conical structure. This pressure component increases the friction between the sealing plug 33 and the channel wall, making the contact between the sealing plug 33 and the channel wall tighter, thereby achieving a highly efficient seal. Under different gas pressure conditions, the conical structure can automatically adjust the sealing effect according to the pressure magnitude, ensuring the reliability of unidirectional gas flow.

[0038] The handle 24 is ergonomically designed with anti-slip textures on its surface to allow operators to easily turn the screw 23. Its shape allows hand muscles to be in a naturally relaxed state, reducing fatigue during long-term operation. The anti-slip textures increase the coefficient of friction between the hand and the handle 24, making it easier for operators to turn the screw 23.

[0039] The outer surface of the storage bottle 11 has graduation lines, which are set based on the precise measurement and division of the internal volume of the storage bottle 11. By measuring the volume of the storage bottle 11 at different heights, the corresponding values ​​are marked as graduations. In this way, the amount of carbon dioxide stored can be accurately measured according to the correspondence between the volume of carbon dioxide gas in the storage bottle 11 and the graduation values.

[0040] The storage bottle 11 is made of transparent or semi-transparent material. The transparent or semi-transparent material of the storage bottle 11 utilizes the light propagation characteristics. Light can pass through these materials, allowing the operator to directly observe the situation inside the storage bottle 11. This visibility provides the operator with intuitive information, which is convenient for operating and monitoring the device.

[0041] Implementation principle: When it is necessary to measure the amount of carbon dioxide injected into the storage bottle 11, the handle 24 is rotated, which drives the screw 23 to rotate. The screw 23 rotates inside the threaded groove 12, causing it to move vertically upward. The movement of the screw 23 drives the fixed disc 21 and the rubber ring 22 to move vertically upward. According to the scale lines on the outer surface of the storage bottle 11, the fixed disc 21 moves to the required position. At this time, when carbon dioxide is injected into the storage bottle 11, the carbon dioxide drives the push plate 25 to move upward. When the push plate 25 moves to the bottom of the fixed disc 21, the amount of carbon dioxide injected into the storage bottle 11 is limited. When it is necessary to discharge the carbon dioxide from the storage bottle 11, the top plate 27 is pushed, causing the top plate 27 to drive the support rod 26 and the push plate 25 to move upward. The push plate 25 can discharge the carbon dioxide from the storage bottle 11.

[0042] When carbon dioxide needs to be added to the storage bottle 11, the bottom plate 35 is pushed upwards, causing the fixed rod 31 and support plate 32 to move upwards. The upward movement of the bottom plate 35 compresses the support spring 36. At this time, a rotational force is applied to the bottom plate 35, causing the fixed rod 31 and support plate 32 to rotate. When the support plate 32 rotates and causes the sealing plug 33 to rotate to the upper end of the vent pipe 38, the force applied to the bottom plate 35 is stopped. Under the elastic force of the support spring 36, the bottom plate 35 returns to its original position, causing the fixed rod 31 and support plate 32 to move upwards. The support plate 32 moves downwards, connecting the vent 34 and the upper end of the inlet pipe 37. The sealing plug 33 seals the upper end of the vent pipe 38, thus preventing carbon dioxide from being discharged from the vent pipe 38 during the filling process and affecting the amount of carbon dioxide added. When it is necessary to discharge carbon dioxide, the above steps are reversed to prevent carbon dioxide from flowing back into the inlet pipe 37 and affecting the amount of carbon dioxide replenished.

[0043] The above are merely preferred embodiments of the present utility model and are not intended to limit the implementation methods and protection scope of the present utility model. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A carbon dioxide supplementation device for plant tissue culture seedlings, characterized in that, include: The storage bottle has an internal gas storage cavity; and the bottom of the storage bottle is provided with an air inlet channel and an air outlet channel. The supplementary components include a fixed disc, a rubber ring, a screw, a handle, a push plate, a support rod, and a top plate. The fixed disc is placed inside the storage bottle, the rubber ring is fixedly connected to the outside of the fixed disc, and the rubber ring forms a dynamic seal with the inner wall of the storage bottle. The screw vertically penetrates the storage bottle and is threadedly connected to a threaded groove at the center of the top of the storage bottle. The bottom end of the screw is rotatably connected to the fixed disc. The handle is fixedly connected to the top of the screw. The push plate is slidably connected to the lower end of the storage bottle. The support rod vertically penetrates the storage bottle and is fixedly connected to the top of the push plate. The top plate is fixedly connected to the top of the support rod. When the screw rotates, it drives the fixed disc to move axially along the storage bottle to compress the storage cavity volume, thereby limiting the initial storage amount of carbon dioxide. The push plate achieves quantitative emission through gas pressure displacement. as well as An anti-backflow mechanism is provided inside the storage bottle to enable unidirectional gas flow during the process of filling the air inlet channel or exhausting the air from the outlet channel.

2. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 1, characterized in that, The anti-backflow mechanism includes a fixed rod, a support plate, a sealing plug, a vent, a base plate, and a support spring. The support plate is movably disposed inside the storage bottle. One end of the fixed rod is fixedly connected to the bottom of the support plate. The fixed rod is vertically inserted and slidably connected to a central channel opened at the center of the bottom of the storage bottle. The base plate is installed on the other end of the fixed rod. The support spring is sleeved on the outside of the fixed rod and is located between the bottom of the storage bottle and the base plate. The sealing plug is fixedly connected to the bottom of the support plate. The vent is opened on the side of the bottom of the support plate away from the sealing plug. The vent and the sealing plug correspond to the positions of the air inlet channel and the air outlet channel, respectively. When the sealing plug closes the air outlet channel, the vent communicates with the air inlet channel; otherwise, it closes the air inlet channel and opens the air outlet channel.

3. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 2, characterized in that, The top end of the support spring is in contact with the bottom end of the storage bottle, and the top end of the support spring is in contact with the top end of the base plate.

4. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 1, characterized in that, An air intake pipe is connected to the air intake channel, and an air outlet pipe is connected to the air outlet channel.

5. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 2, characterized in that, The contact portion of the sealing plug with the air outlet or air inlet channel has a conical structure.

6. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 2, characterized in that, The sealing plug is made of an elastic material.

7. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 1, characterized in that, The handle is ergonomically designed and has anti-slip textures on its surface to facilitate easy rotation of the screw by the operator.

8. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 4, characterized in that, Both the air inlet and outlet pipes are made of corrosion-resistant materials.

9. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 1, characterized in that, The storage bottle has graduation lines on its outer surface.

10. The carbon dioxide supplementation device for plant tissue culture seedlings according to claim 1, characterized in that, The storage bottle is made of transparent or semi-transparent material to facilitate observation of the amount of carbon dioxide stored inside and its working status.

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