Integrated shaft of a cell culture reactor

Abstract

The utility model discloses an integral shaft of cell culture reaction kettle relates to reaction kettle technical field, this cell culture reaction kettle's integral shaft, including the shaft body, the shaft body is passed through jar body setting, and is equipped with three independent interfaces in the upper end of shaft body, is liquid connecting port, gas inlet and gas outlet respectively, liquid connecting port is communicated with jar body inside through liquid extraction channel, and gas inlet is communicated with jar body inside through gas inlet channel, and gas outlet is communicated with jar body inside through gas outlet channel. The utility model discloses a bearing, oil seal new seat and oil seal ring seal the part of shaft body and jar body top contact, form closed cell culture environment, establish liquid extraction channel, gas channel and gas inlet channel on the shaft body simultaneously, ensure the effective extraction of liquid in jar body, the even distribution of gas in jar body and the effective discharge of waste gas from top. Not only has improved the operating efficiency of reaction kettle, also has greatly promoted the reliability of experimental result.

Description

Technical Field

[0001] This invention relates to the field of reaction vessel technology, and in particular to an integrated shaft for a cell culture reaction vessel. Background Technology

[0002] In the fields of biotechnology and pharmaceutical research, cell culture reactors are essential equipment for cell growth and biopharmaceutical production. To ensure environmental control and operational efficiency during cell culture, the reactor design must provide a closed and stable internal environment while allowing for effective liquid extraction, gas exchange, and other functions.

[0003] In traditional designs, insufficient sealing between the reactor shaft and the vessel body easily leads to leakage problems, affecting the accuracy and repeatability of experimental results. Furthermore, the inefficiency of the liquid extraction and gas exchange systems also limits the reactor's operational performance. Existing solutions to these problems typically include complex sealing structures and diverse piping connections, but these methods often increase equipment cost and maintenance complexity, while failing to completely resolve issues of inadequate sealing and unstable connections.

[0004] In view of this, an integral shaft for a cell culture reactor is proposed to solve the above problems. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the defects of the prior art and provide an integrated shaft for a cell culture reactor.

[0006] The present invention solves the above-mentioned technical problems through the following technical solution:

[0007] An integral shaft for a cell culture reactor includes a shaft body that extends through the tank body. The shaft body has three independent interfaces at its upper end: a liquid connection port, an air inlet, and an air outlet. The liquid connection port is connected to the inside of the tank body through a liquid extraction channel, the air inlet is connected to the inside of the tank body through an air inlet channel, and the air outlet is connected to the inside of the tank body through an air outlet channel. The three channels do not interfere with each other within the shaft body.

[0008] Preferably, the upper end of the shaft extends beyond the upper end of the tank body, the lower side of the tank body is provided with a tank bottom, and the upper end of the shaft body cooperates with the new oil seal seat through a bearing and is sealed by an oil seal ring. The new oil seal seat is fitted inside the top of the tank body to realize the assembly of the entire closed reaction vessel.

[0009] Preferably, the liquid extraction channel has a length adapted to extend into the tank to ensure effective extraction of the culture supernatant.

[0010] Preferably, the length of the air intake channel is such that the gas can be evenly distributed within the tank without directly impacting the reactants.

[0011] Preferably, the length of the gas outlet channel is such that gas can be effectively discharged from the top of the tank.

[0012] Preferably, the shaft is injection molded from PK plastic.

[0013] Preferably, the liquid connection port, air inlet, and air outlet are all provided with external threads on their outer sides.

[0014] Preferably, pipe positioning components are installed at the ends of the liquid connection port, air inlet, and air outlet.

[0015] Preferably, the pipe positioning assembly includes an arc-shaped sleeve one and an arc-shaped sleeve two, with one end of the arc-shaped sleeve one and the other end connected by bolts.

[0016] Preferably, one side of each of the arc-shaped sleeve one and arc-shaped sleeve two is provided with multiple side plates, and a screw is threadedly connected to the side plate, with a positioning block rotatably connected to one end of the screw.

[0017] The positive and progressive effects of this utility model are as follows:

[0018] 1. This invention seals the contact area between the shaft and the top of the tank using bearings, a new oil seal seat, and an oil seal ring, creating a closed cell culture environment. Simultaneously, liquid extraction, gas outlet, and gas inlet channels are provided on the shaft, ensuring effective extraction of liquid from the tank, uniform gas distribution within the tank, and effective exhaust of waste gas from the top. This design not only improves the operating efficiency of the reactor but also significantly enhances the reliability and repeatability of experimental results.

[0019] 2. This utility model increases the friction with the connecting pipe by setting external threads on the outside of the liquid connection port, air inlet and air outlet, making the pipe connection tighter. At the same time, the arc sleeve one and arc sleeve two are fixed at these connection ports by bolts, and the connecting pipe is fixed by the screw with the positioning block, further preventing the connecting pipe from falling off. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the shaft installation state according to an embodiment of the present utility model;

[0021] Figure 2 This is a schematic diagram of the shaft at the upper end of the tank in an embodiment of the present invention;

[0022] Figure 3 This is a schematic diagram of the shaft at the lower end of the tank in an embodiment of the present invention;

[0023] Figure 4 This is a schematic diagram of the shaft and other structures in an embodiment of the present utility model;

[0024] Figure 5 This is a top view of the shaft structure according to an embodiment of the present utility model;

[0025] Figure 6 This is a schematic diagram of the internal structure of the shaft in an embodiment of the present utility model;

[0026] Figure 7 This is a schematic diagram of the liquid extraction channel and other structures in an embodiment of the present invention;

[0027] Figure 8 This is a schematic diagram of the pipe fixing mechanism according to an embodiment of the present utility model.

[0028] Figure label:

[0029] 1. Shaft; 2. New oil seal seat; 3. Bearing; 4. Liquid connection port; 5. Air inlet; 6. Air outlet; 7. Liquid extraction channel; 8. Air inlet channel; 9. Air outlet channel; 10. Arc sleeve one; 11. Arc sleeve two; 12. Bolt; 13. Side plate; 14. Screw; 15. Positioning block; 16. Tank body; 17. Tank bottom; 18. External thread; 19. Oil seal ring. Detailed Implementation

[0030] The following detailed description, in conjunction with the accompanying drawings, outlines some embodiments of the present invention. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0031] Example 1

[0032] Please refer to Figures 1-7 This utility model embodiment provides an integrated shaft for a cell culture reactor, including a shaft body 1. One end of the shaft body 1 penetrates through the tank body 16 and extends to a position near the bottom 17 of the tank, while the other end extends out of the upper end of the tank body 16. The upper end of the shaft body 1 is engaged with an oil seal seat 2 via a bearing 3, and a sealing effect is achieved through an oil seal ring 19. The oil seal seat 2 is installed inside the top of the tank body 16 to ensure the sealing of the entire cell culture reactor.

[0033] The shaft body 1 is manufactured using PK plastic through injection molding, exhibiting good mechanical strength and chemical corrosion resistance. At the upper end of the shaft body 1, a liquid connection port 4, an air inlet 5, and an air outlet 6 are respectively provided. These ports are connected to the liquid extraction channel 7, air inlet channel 8, and air outlet channel 9 inside the shaft body 1, respectively, for realizing gas exchange and culture medium extraction operations within the sealed reactor.

[0034] Specifically, the liquid extraction channel 7 is designed to be of an appropriate length so that it can penetrate deep into the tank 16 to ensure that the supernatant of the culture medium can be effectively extracted; the length of the gas inlet channel 8 is optimized to ensure that the gas can be evenly distributed in the tank 16 and avoid direct impact on the reactants; while the length of the gas outlet channel 9 ensures that the exhaust gas can be efficiently discharged from the top of the tank 16 to maintain the stability of the internal environment of the reactor.

[0035] Furthermore, to enhance the stability and sealing of the pipe connections, external threads 18 are provided on the outer sides of the liquid connection port 4, the air inlet 5, and the air outlet 6. This design increases the friction between the interface and the external pipe, resulting in a tighter connection and reducing the risk of leakage. These design features work together to not only improve the operating efficiency of the reactor but also enhance the reliability and repeatability of experimental results.

[0036] Example 2

[0037] like Figure 4 and Figure 8 As shown, pipe positioning components are installed at the ends of liquid connection port 4, air inlet port 5, and air outlet port 6. These components are used to securely connect external pipes to these interfaces and work in conjunction with the external thread 18 to further enhance the stability and sealing of the pipe connection.

[0038] Specifically, the pipe positioning assembly consists of an arc-shaped sleeve 10 and an arc-shaped sleeve 11. One end of the arc-shaped sleeve 10 and the arc-shaped sleeve 11 are connected by a hinge, and the other end is fixed by a bolt 12, thereby tightly installing the arc-shaped sleeve 10 and the arc-shaped sleeve 11 at the ends of the liquid connection port 4, the air inlet port 5, and the air outlet port 6. This design facilitates quick installation and disassembly while ensuring effective fixation of the pipe.

[0039] In addition, multiple side plates 13 are respectively provided on one side of the arc-shaped sleeve 10 and the arc-shaped sleeve 11, and each side plate 13 is threaded with a screw 14. One end of the screw 14 is rotatably connected to the positioning block 15. After the external pipe is in place, the positioning block 15 is moved towards the pipe and finally fixed to the outside of the pipe by rotating the screw 14. The positioning block 15 adopts an arc-shaped structure that matches the shape of the pipe, and its inner side is equipped with a soft pad. This design not only improves the stability of the pipe connection, but also effectively avoids damage to the pipe surface due to direct contact, thereby extending the service life of the pipe and ensuring the safety of the connection.

[0040] In summary, the integrated shaft of the cell culture reactor of this utility model operates on the following principle: liquid extraction and gas exchange within the cell culture reactor are achieved through the liquid connection port 4, air inlet 5, and air outlet 6 on the shaft body 1. These interfaces are connected to the internal liquid extraction channel 7, air inlet channel 8, and air outlet channel 9, ensuring operational effectiveness and environmental stability. The external threads 18 on the outer side of the interfaces enhance the tightness of the pipe connection, while the pipe positioning assembly installed at the end of the interfaces further reinforces the connection, preventing loosening and leakage. This assembly utilizes the synergistic action of the arc-shaped sleeve 10, arc-shaped sleeve 11, bolt 12, screw 14, and positioning block 15 to precisely fix the external pipes, ensuring the system's sealing and long-term stable operation.

[0041] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An integral shaft for a cell culture reactor, characterized in that: Includes a shaft (1), which is installed through the tank (16). The upper end of the shaft (1) has three independent interfaces, namely a liquid connection port (4), an air inlet (5), and an air outlet (6). The liquid connection port (4) is connected to the inside of the tank (16) through a liquid extraction channel (7). The air inlet (5) is connected to the inside of the tank (16) through an air inlet channel (8). The air outlet (6) is connected to the inside of the tank (16) through an air outlet channel (9). The three channels do not interfere with each other within the shaft (1).

2. The integral shaft of the cell culture reactor according to claim 1, characterized in that: The upper end of the shaft (1) extends out of the upper end of the tank (16). The tank (16) has a tank bottom (17) on its lower side. The upper end of the shaft (1) is engaged with the new oil seal seat (2) through the bearing (3) and sealed by the oil seal ring (19). The new oil seal seat (2) is fitted inside the top of the tank (16) to realize the assembly of the entire closed reactor.

3. The integral shaft of the cell culture reactor according to claim 2, characterized in that: The liquid extraction channel (7) has a length adapted to extend into the tank (16) to ensure effective extraction of culture supernatant.

4. The integral shaft of the cell culture reactor according to claim 3, characterized in that: The length of the air intake channel (8) is such that the gas can be evenly distributed in the tank (16) without directly impacting the reactants.

5. The integral shaft of the cell culture reactor according to claim 4, characterized in that: The length of the venting channel (9) ensures that gas can be effectively discharged from the top of the tank (16).

6. The integral shaft of the cell culture reactor according to claim 1, characterized in that: The shaft (1) is injection molded and made of PK plastic.

7. The integral shaft of the cell culture reactor according to claim 6, characterized in that: External threads (18) are provided on the outside of the liquid connection port (4), air inlet (5) and air outlet (6).

8. The integral shaft of the cell culture reactor according to claim 7, characterized in that: Pipe positioning components are installed at the ends of the liquid connection port (4), air inlet (5) and air outlet (6).

9. The integral shaft of the cell culture reactor according to claim 8, characterized in that: The pipe positioning assembly includes an arc-shaped sleeve one (10) and an arc-shaped sleeve two (11), with one end of the arc-shaped sleeve one (10) and the other end connected by a bolt (12).

10. The integral shaft of the cell culture reactor according to claim 9, characterized in that: Multiple side plates (13) are respectively provided on one side of the arc sleeve one (10) and arc sleeve two (11). A screw (14) is threaded on the side plate (13), and a positioning block (15) is rotatably connected to one end of the screw (14).

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