Cell online microscopic observation system

Abstract

This application belongs to the field of microscopic observation technology, specifically disclosing an online cell microscopic observation system, including a body. Within the body are: a support mechanism for supporting a culture vessel; a microscopic mechanism for performing bright-field and fluorescence observations of the analyte in the culture vessel; a switchable bright-field light source for providing illumination for bright-field observations, the bright-field light source being located above the culture vessel; a switchable fluorescence light source for providing excitation light for fluorescence observations, the fluorescence light source and the microscopic mechanism being located below the culture vessel; and a displacement mechanism for driving the movement of the microscopic mechanism, the bright-field light source, and the fluorescence light source. The observation system of this application can simultaneously meet the needs of bright-field and fluorescence observations, and the operation process is simple, making it suitable for batch microscopic observations.

Description

Technical Field

[0001] This application belongs to the field of microscopic observation technology, and more specifically, relates to an online cell microscopic observation system. Background Technology

[0002] Cell and microbial culture is a common experimental method in the biotechnology industry. Due to its stringent requirements for the culture environment, it is usually carried out in a specific culture vessel, which makes real-time observation of cells and microorganisms inconvenient.

[0003] like Figure 1 As shown, in related technologies, to observe the culture status, researchers frequently need to remove cultures from culture vessels such as well plates, culture flasks, and culture dishes to prepare samples, and then place the samples under a microscope for observation. Especially when using culture vessels such as 96-well plates, large-scale sampling and observation recording are required. This process is not only cumbersome and labor-intensive, but also carries risks such as culture infection and sample contamination after sampling. Furthermore, changes in the culture environment caused by sampling can easily lead to errors in the culture results, and in severe cases, even cell death. In addition, due to different observation needs, researchers often need to place samples in different types of microscopes for bright-field or fluorescence observation, which is inconvenient and urgently needs improvement. Utility Model Content

[0004] In response to the shortcomings or improvement needs of existing technologies, this application provides an online cell microscopy observation system, which aims to improve the problems of cumbersome traditional microscopy observation procedures, such as culture infection, sample contamination, and easy errors in culture results.

[0005] This application provides an online cell microscopy observation system, comprising an organism, wherein the organism is equipped with:

[0006] Support mechanism for supporting culture apparatus;

[0007] Microscopic apparatus used for bright-field and fluorescence observation of analytes in a culture vessel;

[0008] A switchable bright-field light source for providing illumination for bright-field observation, the bright-field light source being located above the incubator;

[0009] A switchable fluorescence light source for providing excitation light for fluorescence observation, wherein both the fluorescence light source and the microstructure are located below the incubator;

[0010] A displacement mechanism for driving the movement of the microscopic mechanism, bright field light source, and fluorescent light source so that the microscopic mechanism can observe the analyte in a stationary culture vessel.

[0011] Compared with the prior art, the technical solution conceived in this application adopts a design scheme in which the culture vessel is placed still while the microscopic mechanism and light source move for microscopic observation. This eliminates the need for experimenters to take samples for observation, greatly avoiding problems such as culture infection, sample contamination, and errors in culture results due to changes in the culture environment that may occur during the sampling process, thus making the culture observation results more accurate.

[0012] In particular, for batch culture experiments such as 96-well plates, the microscopic mechanism can be moved by a displacement mechanism to conduct microscopic observations of different areas. This design optimizes the drawback of traditional designs that require experimenters to repeatedly take samples, observe and record a large number of times, reducing the labor intensity of experimenters and ensuring batch consistency.

[0013] Furthermore, this design enables both bright and dark field compatible observations, simultaneously meeting the needs of both bright field and fluorescence observations, greatly expanding the usability and scalability of the observation system.

[0014] As a further preferred embodiment, the microscopic mechanism includes an objective lens, a filter assembly, a mirror assembly, a tube mirror, and a camera. The light signal output from the bright field light source or the fluorescent light source acts on the incubator and then passes sequentially through the objective lens, the filter assembly, the mirror assembly, and the tube mirror to reach the camera, forming an overall L-shaped right-angle light path.

[0015] As a further preferred embodiment, the fluorescent light source is connected to the microscope mechanism, and the fluorescent light source is located beside the objective lens and tilted upwards.

[0016] As a further preferred embodiment, multiple fluorescent light sources are uniformly arranged around the objective lens.

[0017] As a further preferred embodiment, the displacement mechanism includes:

[0018] A first displacement submechanism for driving the displacement of the microstructure;

[0019] A second displacement sub-mechanism for driving the displacement of the bright field light source.

[0020] As a further preferred embodiment, the first displacement submechanism is a three-axis motion platform.

[0021] As a further preferred embodiment, the supporting mechanism includes a frame and a light-transmitting plate, the frame being disposed inside the machine body, and the light-transmitting plate being disposed on the frame and used to support the incubator.

[0022] As a further preferred embodiment, the supporting mechanism also includes a positioning fixture for positioning the culture vessel disposed on the light-transmitting plate.

[0023] As a further preferred embodiment, the observation system also includes a protective housing for protecting the bright-field light source.

[0024] As a further preferred embodiment, the body includes a housing and an adjustable dark field cover disposed on the housing.

[0025] In summary, compared with the prior art, the technical solutions conceived in this application have the following main technical advantages:

[0026] 1. This application adopts a design scheme in which the culture vessel is placed still while the microscopic mechanism and light source move for microscopic observation. This eliminates the need for experimenters to take samples for observation, greatly avoiding problems such as culture infection, sample contamination, and errors in culture results due to changes in the culture environment that may occur during the sampling process, thus making the observation results more accurate.

[0027] 2. This design allows for the movement of the microscopic mechanism via a displacement mechanism, enabling microscopic observation of different areas within the culture vessel. This eliminates the need for researchers to perform repetitive, large-scale sampling and observation, reducing workload and helping to maintain consistency in batch observations.

[0028] 3. This design enables bright-field and dark-field compatible observation, which can simultaneously meet the needs of bright-field and fluorescence observation, greatly expanding the usability and scalability of the observation system. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of an existing microscope mentioned in the background art of this application;

[0030] Figure 2 This is a schematic diagram of the structure of the online cell microscopy observation system provided in the embodiments of this application;

[0031] Figure 3 This is a cross-sectional view of the online cell microscopy observation system provided in the embodiments of this application;

[0032] Figure 4 This is a schematic diagram of the bearing mechanism, microscopic mechanism, and displacement mechanism provided in the embodiments of this application;

[0033] Figure 5 This is a schematic diagram of the X-axis motion component provided in an embodiment of this application;

[0034] Figure 6 This is a schematic diagram of the Y-axis motion component provided in an embodiment of this application;

[0035] Figure 7 This is a schematic diagram of the layout of the microstructure, fluorescent light source, and Z-axis motion assembly provided in the embodiments of this application;

[0036] Figure 8 This is a schematic diagram of the layout of the bright field light source, the second displacement sub-mechanism, and the protective shell provided in the embodiments of this application;

[0037] Figure 9 This is a schematic diagram of the structure of the bearing mechanism provided in the embodiments of this application;

[0038] Figure 10 This is a schematic diagram of the electrical components provided in the embodiments of this application.

[0039] In all the accompanying drawings, the same reference numerals are used to denote the same elements or structures, wherein:

[0040] 1. Body; 1-1. Housing; 1-2. Dark Field Cover; 1-3. Communication Board; 2. Support Mechanism; 2-1. Frame; 2-1a. Front Support; 2-1b. Base Plate; 2-1c. Rear Support; 2-2. Light Transmitting Plate; 2-3. Positioning Fixture; 3. Microscopic Mechanism; 3-1. Objective Lens; 3-2. Filter Assembly; 3-3. Mirror Assembly; 3-4. Tube Lens; 3-5. Camera; 4. Bright Field Light Source; 5. Fluorescent Light Source; 6. Protective Housing; 7. X-Axis Motion Assembly; 7-1. X-Axis Motor; 7-2. X-Axis Coupling; 7-3. XY Connector; 7-4. X-Axis Guide Rail; 7-5. X-Axis Cable Chain; 7-6 7-7 X-axis lead screw holder; 8. Y-axis lead screw; 8-1 Y-axis motor; 8-2 Y-axis coupling; 8-3 YZ connector; 8-4 Y-axis guide rail; 8-5 Y-axis drag chain; 8-6 Y-axis lead screw holder; 8-7 Y-axis lead screw; 9. Z-axis motion assembly; 9-1 Z-axis linear power component; 9-2 Z-axis mounting plate; 9-3 Z-axis connecting plate; 10. Second displacement sub-mechanism; 11. Electrical assembly; 11-1 Electrical mounting plate; 11-2 Terminal assembly; 11-3 Solenoid valve assembly; 11-4 Motor drive; 11-5 Motion controller; 100. Incubator. Detailed Implementation

[0041] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0042] The following is in conjunction with the appendix Figures 2-10 This application will be described in further detail.

[0043] This application discloses an online cell microscopy observation system. (Refer to...) Figures 2-4The online cell microscopy observation system includes a body 1, within which are a support mechanism 2, a microscopic mechanism 3, a displacement mechanism, a bright-field light source 4, and a fluorescence light source 5. The support mechanism 2 supports the culture vessel 100, and the microscopic mechanism 3 is used for bright-field and fluorescence observation of the analytes in the culture vessel 100. Both the bright-field light source 4 and the fluorescence light source 5 are adjustable. The bright-field light source 4 is located above the culture vessel 100 and provides illumination for bright-field observation, while the fluorescence light source 5 and the microscopic mechanism 3 are located below the culture vessel 100. The fluorescence light source 5 provides excitation light for fluorescence observation. The displacement mechanism drives the movement of the microscopic mechanism 3, the bright-field light source 4, and the fluorescence light source 5.

[0044] With this design, by setting up a support mechanism 2, a microscopic mechanism 3, a displacement mechanism and a light source inside the body 1, the experimenter can perform bright-field observation and fluorescence observation of the test analyte in the culture vessel 100 based on this system. This optimizes the sampling steps required for traditional observation and greatly avoids the risks of culture infection, changes in the culture environment and sample contamination after sampling during the sampling process, making the culture observation results more accurate.

[0045] When conducting batch culture experiments such as 96-well plates, the position of the microscopic mechanism 3 and the lamp source can be adjusted by the displacement mechanism to quickly observe specific parts. This design optimizes the drawback of traditional designs that require experimenters to repeatedly take a large number of samples for observation and recording, greatly improving the labor intensity of experimenters, and also providing strong evidence for batch consistency.

[0046] Furthermore, this design is a bright-field and dark-field compatible observation scheme, which can simultaneously meet the needs of customers conducting both bright-field and fluorescence observations, greatly expanding the system's usability and scalability. Moreover, the system in this design has a high degree of integration, a small overall size and weight, and is suitable for desktop placement, making it convenient for laboratory personnel to move or transport, representing a significant advancement.

[0047] Furthermore, such as Figure 2 As shown, in some embodiments, the body 1 includes a housing 1-1 and an adjustable dark field cover 1-2 disposed on the housing 1-1, the dark field cover 1-2 being used to provide a dark field environment for fluorescence observation.

[0048] In actual use, the experimenter can open the dark field cover 1-2 and place the culture vessel 100 into the body 1, or take out the culture vessel 100 from the body 1, or perform adjustments, repairs and other work on the internal mechanism of the body 1.

[0049] Furthermore, the body 1 is equipped with control buttons and heat dissipation structures, and the side wall of the body 1 is equipped with a communication board 1-3, which is used to install the equipment power socket and external communication interface and other devices.

[0050] Furthermore, in some embodiments, the fluorescent light source 5 is connected to the microscopic mechanism 3, and the displacement mechanism includes: a first displacement sub-mechanism for driving the displacement of the microscopic mechanism 3, and a second displacement sub-mechanism 10 for driving the displacement of the bright-field light source 4. The first displacement sub-mechanism includes, but is not limited to, employing a multi-axis motion platform, and the second displacement sub-mechanism 10 includes, but is not limited to, employing an existing linear motion mechanism or a multi-degree-of-freedom motion mechanism. In some other embodiments, the fluorescent light source 5 may also be separately configured from the microscopic mechanism 3; in this case, the displacement mechanism should also include a third displacement sub-mechanism for driving the displacement of the fluorescent light source 5.

[0051] Specifically, such as Figure 4 As shown, in some embodiments, the first displacement submechanism includes a three-degree-of-freedom translational motion platform, which may specifically include an X-axis motion component 7, a Y-axis motion component 8, and a Z-axis motion component 9. The X-axis motion component 7, Y-axis motion component 8, and Z-axis motion component 9 may include, but are not limited to, linear motor modules, cylinders, linear motors, etc.

[0052] Preferably, both the X-axis motion assembly 7 and the Y-axis motion assembly 8 employ high-precision motor-screw combinations, with each axis equipped with two high-precision guide rails. The X-axis is configured with a "gantry" type double-sided guide rail, while the Y-axis is configured with a "right-angle" type double guide rail, ensuring high stability and high precision during operation. Of course, in some other embodiments, the multi-axis motion platform can also be a motion mechanism with four degrees of freedom or even more degrees of freedom.

[0053] Specifically, such as Figure 5 As shown, in some embodiments, the X-axis motion assembly 7 includes an X-axis motor 7-1, an X-axis coupling 7-2, an X-axis lead screw assembly, an XY connector 7-3, an X-axis guide rail 7-4, and an X-axis cable chain 7-5. The X-axis lead screw assembly includes an X-axis lead screw seat 7-6 and an X-axis lead screw 7-7. The X-axis lead screw seat 7-6 is fixed inside the machine body 1, and the X-axis lead screw 7-7 is rotatably connected to the X-axis lead screw seat 7-6. The X-axis motor 7-1 is fixedly mounted and connected to the X-axis lead screw 7-7 via the X-axis coupling 7-2. Two X-axis guide rails 7-4 are provided, both serving to guide the X-axis motion assembly 7. The XY connector 7-3 is anti-rotationally mounted on the X-axis lead screw 7-7 and also connects to the Y-axis motion assembly 8. The X-axis cable chain 7-5 is used to house the cables required for the X-axis motion assembly 7.

[0054] In actual use, the X-axis motor 7-1 drives the X-axis lead screw 7-7 to rotate through the X-axis coupling 7-2. Then, the X-axis lead screw 7-7 drives the XY connector 7-3 to move linearly along the X-axis, thereby driving the Y-axis motion component 8 to move along the X-axis.

[0055] like Figure 6As shown, in some embodiments, the Y-axis motion assembly 8 includes a Y-axis motor 8-1, a Y-axis coupling 8-2, a Y-axis lead screw assembly, a YZ connector 8-3, a Y-axis guide rail 8-4, and a Y-axis drag chain 8-5. The Y-axis motor 8-1 provides power for Y-axis movement. The Y-axis motor 8-1 is connected to the Y-axis lead screw assembly through the Y-axis coupling 8-2. The Y-axis lead screw assembly includes a Y-axis lead screw seat 8-6 and a Y-axis lead screw 8-7. The operating logic of the Y-axis motion assembly 8 is basically similar to that of the X-axis motion assembly 7. The rotational motion is converted into linear motion of the YZ connector 8-3 through the Y-axis lead screw assembly, and the YZ connector 8-3 drives the Z-axis motion assembly 9 to perform linear movement along the Y-axis.

[0056] like Figure 7 As shown, in some embodiments, the Z-axis motion assembly 9 includes a Z-axis linear actuator 9-1, which includes, but is not limited to, electric actuators, pneumatic actuators, etc., and is used to drive the microscopic mechanism 3 to perform Z-axis linear movement. The Z-axis motion assembly 9 is connected to the YZ connector 8-3 via a Z-axis mounting plate 9-2, and is also connected to the microscopic mechanism 3 via a Z-axis connecting plate 9-3.

[0057] Furthermore, in some embodiments, the second displacement sub-mechanism 10 is used to drive the bright field light source 4 to move along the Y-axis. The second displacement sub-mechanism 10 includes, but is not limited to, linear modules, electric actuators, etc. Preferably, such as... Figure 8 As shown, in some embodiments, the second displacement submechanism 10 is installed inside the body 1 via a support base, and it drives the bright field light source 4 to move by means of a lead screw drive. It is worth noting that the second displacement submechanism 10 has an opening reserved to allow the bright field light source 4 to transmit light downwards.

[0058] As a preferred option, such as Figure 8 As shown, the observation system also includes a protective shell 6 for protecting the bright field light source 4. The protective shell 6 is installed on the upper end of the support base. The protective shell 6 adopts an inverted one-piece "L" shape, which satisfies both functionality and aesthetics.

[0059] Furthermore, such as Figure 7 As shown, in some embodiments, the microscopic mechanism 3 includes a camera 3-5, a tube lens 3-4, a mirror assembly 3-3, a filter assembly 3-2, and an objective lens 3-1. The light signal output by the bright field light source 4 or the fluorescent light source 5 acts on the incubator 100 and can be collected by the objective lens 3-1, filtered by the filter assembly 3-2, reflected by the mirror assembly 3-3, and focused by the tube lens 3-4 to reach the camera 3-5, and imaged by the camera 3-5.

[0060] The microscope tube 3-4 is preferably a microscope tube. The mirror assembly 3-3 is used to reflect the light signal at a 90-degree angle, and includes a microscope tube and a mirror. The filter assembly 3-2 includes a lens tube and a filter. By using the combination of objective lens 3-1, microscope tube 3-4, camera 3-5, and mirror, a high-resolution and wide-field-of-view effect is achieved, forming an overall "L"-shaped right-angle optical path. The relevant microscopy and imaging principles are existing technologies and will not be elaborated further here.

[0061] Furthermore, such as Figure 7 As shown, in some embodiments, the fluorescence light source 5 is connected to the microscope mechanism 3, and the fluorescence light source 5 is located beside the objective lens 3-1 and tilted upwards. Preferably, multiple fluorescence light sources 5 are evenly arranged around the objective lens 3-1; for example, two fluorescence light sources 5 are provided and respectively located on both sides of the axial direction of the objective lens 3-1. In this design, a lighting scheme that synchronizes the bright and dark field light sources with the lens is adopted. The bright field light source 4 is a transmissive coaxial light source, and the fluorescence light source 5 is a non-coaxial tilted light source.

[0062] Furthermore, such as Figure 9 As shown, in some embodiments, the supporting mechanism 2 includes a frame 2-1 and a light-transmitting plate 2-2. The frame 2-1 is installed inside the body 1, and the light-transmitting plate 2-2 is installed on the frame 2-1 and used to support the incubator 100.

[0063] The frame 2-1 includes a front support 2-1a, a base plate 2-1b, and a rear support 2-1c, which are connected sequentially to form a gate-shaped support. The base plate 2-1b has a through mounting opening on its surface, and a light-transmitting plate 2-2 is embedded in the mounting opening. The light-transmitting plate 2-2 is preferably optical glass.

[0064] Furthermore, in some embodiments, the supporting mechanism 2 also includes a positioning fixture 2-3 disposed on the light-transmitting plate 2-2 for positioning the culture device 100, the positioning fixture 2-3 including but not limited to an L-shaped positioning plate placed on the light-transmitting plate 2-2.

[0065] Furthermore, in some embodiments, the observation system also includes an electrical component 11, which is used to control devices such as displacement mechanisms, motion mechanisms, and light sources.

[0066] As a preferred option, such as Figure 10As shown, the electrical assembly 11 includes an electrical mounting plate 11-1, a terminal assembly 11-2, a solenoid valve assembly 11-3, a motor drive 11-4, and a motion controller 11-5. The electrical mounting plate 11-1 is used to mount the electrical circuits; the terminal assembly 11-2 is used to connect the wiring of each electrical component; the solenoid valve assembly 11-3 is used to control the light source; and the motor drive 11-4 is used to control the motor. The motion controller 11-5, as the core control device of the entire equipment, is used to control the operation of the remaining components and drives.

[0067] In this design, electrical component 11 adopts an integrated control circuit, with motion controller 11-5 as the main control core, communicating with three motor drives 11-4 to complete motion planning. The overall power supply primarily uses low voltage, supplying power to the motors, bright field light source 4, fluorescent light source 5, and sensors via terminal assembly 11-2, thus improving system safety. This design significantly reduces the size of the control box, while making the overall control system more centralized. The relevant control principles are existing technology and will not be elaborated upon here.

[0068] It should be understood that expressions such as "comprising" and "may include" as used in this application indicate the existence of the disclosed functions, operations, or constituent elements, and do not limit one or more additional functions, operations, and constituent elements. In this application, terms such as "comprising" and / or "having" may be interpreted as indicating a specific characteristic, number, operation, constituent element, component, or combination thereof, but should not be interpreted as excluding the existence or possibility of adding one or more other characteristics, numbers, operations, constituent elements, components, or combinations thereof.

[0069] It should be understood that the terms “center,” “upper,” “lower,” “front,” “rear,” “left,” “right,” “vertical,” “horizontal,” “inner,” “outer,” “clockwise,” “counterclockwise,” “axial,” “radial,” and “circumferential” indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0070] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0071] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0072] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A cell online microscopic observation system, characterized in that, Includes a body (1), wherein the body (1) is provided with: Support mechanism (2) for supporting the culture vessel (100); Microscopic apparatus (3) for bright-field and fluorescence observation of analytes in culture vessel (100); A switchable bright field light source (4) for providing illumination for bright field observation, the bright field light source (4) being located above the incubator (100); A switchable fluorescence light source (5) for providing excitation light for fluorescence observation, wherein the fluorescence light source (5) and the microstructure (3) are both located below the incubator (100); A displacement mechanism for driving the movement of the microscopic mechanism (3), the bright field light source (4) and the fluorescent light source (5) so that the microscopic mechanism (3) can observe the analyte in the stationary culture vessel (100).

2. The online cell microscopy observation system as described in claim 1, characterized in that, The microscopic mechanism (3) includes an objective lens (3-1), a filter assembly (3-2), a mirror assembly (3-3), a tube mirror (3-4), and a camera (3-5). The light signal output by the bright field light source (4) or the fluorescent light source (5) acts on the incubator (100) and then passes through the objective lens (3-1), the filter assembly (3-2), the mirror assembly (3-3), and the tube mirror (3-4) in sequence to reach the camera (3-5), forming a right-angled light path in an L-shape.

3. The online cell microscopy observation system as described in claim 2, characterized in that, The fluorescent light source (5) is connected to the microscope mechanism (3), and the fluorescent light source (5) is located on the side of the objective lens (3-1) and tilted upwards.

4. The online cell microscopy observation system as described in claim 3, characterized in that, Multiple fluorescent light sources (5) are uniformly arranged around the objective lens (3-1).

5. The online cell microscopy observation system as described in claim 3, characterized in that, The displacement mechanism includes: A first displacement sub-mechanism for driving the displacement of the micromechanism (3); The second displacement submechanism (10) is used to drive the displacement of the bright field light source (4).

6. The online cell microscopy observation system as described in claim 5, characterized in that, The first displacement submechanism is a three-axis motion platform.

7. The online cell microscopy observation system as described in claim 1, characterized in that, The supporting mechanism (2) includes a frame (2-1) and a light-transmitting plate (2-2). The frame (2-1) is located inside the machine body (1), and the light-transmitting plate (2-2) is located on the frame (2-1) and is used to support the incubator (100).

8. The online cell microscopy observation system as described in claim 7, characterized in that, The supporting mechanism (2) also includes a positioning fixture (2-3) for positioning the culturer (100) disposed on the light-transmitting plate (2-2).

9. The online cell microscopy observation system according to any one of claims 1-8, characterized in that, The observation system also includes a protective shell (6) for protecting the bright field light source (4).

10. The online cell microscopy observation system according to any one of claims 1-8, characterized in that, The body (1) includes a housing (1-1) and an adjustable dark field cover (1-2) disposed on the housing (1-1).

Images