A multi-functional nucleic acid purification column adapter

Abstract

The utility model discloses a multifunctional nucleic acid purification column adapter, including connecting column, the connecting column top is fixed with the transition column, the transition column top is fixed with the installation column, the multifunctional nucleic acid purification column adapter still includes: sealed connection structure, sealed connection structure sets up in the transition column and the installation column junction. Inserting the flat bottom purification column into the installation column of the adapter, and turning into the negative pressure suction filtration mode, separating the adapter and the purification column, and turning the purification column into the angular rotor centrifugal mode, one column dual -use, high -efficient processing liquid under the negative pressure suction filtration, very low solution residue under high -speed centrifugal, silica gel ring + taper double -sealed, residual amount <20ul, and the adapter cost is about 1 / 5 purification column, and can be repeatedly used, install the sealing washer in the inner extension platform of the transition column and the installation column junction, and the limiting platform of the inner extension platform edge and the inner wall of the sealing washer abut, can increase the stability of the sealing washer.

Description

Technical Field

[0001] This application relates to the field of biological experimental equipment technology, specifically a multifunctional nucleic acid purification column adapter. Background Technology

[0002] Nucleic acid purification columns are core equipment used in molecular biology experiments for the separation and purification of nucleic acids. They achieve efficient adsorption and elution of nucleic acids through a combination of centrifugal adsorption and a silica membrane. The main structure consists of a collection tube, adsorption column, and filter membrane, and is made of materials such as polypropylene to meet aseptic requirements. It is compatible with centrifuges of different sizes for standardized operation. Purification columns come in two forms: a flat-bottomed structure with numerous pores at the bottom, allowing solution to pass through smoothly, suitable for high-speed centrifugation with an angle rotor; and a pointed-bottomed structure that can be inserted into a filtration device. In a nucleic acid extraction process, the advantages of high-speed centrifugation with the flat-bottomed column and the negative pressure filtration with the pointed-bottomed column can be fully utilized to improve nucleic acid extraction quality, yield, efficiency, and operational flexibility.

[0003] Flat-bottomed purification columns have filter holes at the bottom, making them compatible with angle rotor high-speed centrifuges and eliminating dead zones. However, they cannot be connected to negative pressure devices, resulting in slow operation speeds for large-volume solutions. Conical-bottomed purification columns can be inserted into negative pressure devices for rapid filtration of large-volume solutions, but due to structural limitations, dead zones exist when using angle rotor high-speed centrifugation, leading to significant solution residue. Furthermore, due to size limitations, the structure of conical-bottomed purification columns cannot be improved to eliminate dead zones, thus making them unsuitable for angle rotor high-speed centrifugation. Consequently, they cannot fully leverage the advantages of both high-speed centrifugation and negative pressure filtration for nucleic acid purification, forcing the use of only one of these methods for nucleic acid extraction.

[0004] Therefore, it is desirable to provide a multifunctional nucleic acid purification column adapter. Utility Model Content

[0005] This embodiment provides a multifunctional nucleic acid purification column adapter, which solves the problem that it is impossible to fully utilize the advantages of both high-speed centrifugation and negative pressure filtration for nucleic acid purification, thus limiting the use of only one of these methods for nucleic acid extraction.

[0006] According to one aspect of this application, a multifunctional nucleic acid purification column adapter is provided, including a connecting column, a transition column fixed to the top of the connecting column, and a mounting column fixed to the top of the transition column. The multifunctional nucleic acid purification column adapter further includes:

[0007] A sealed connection structure is provided at the connection between the transition column and the mounting column.

[0008] Furthermore, the outer diameter of the bottom end of the connecting post is set to 4.2 mm, and the outer diameter of the top end of the connecting post is set to 4.5 mm.

[0009] Furthermore, the height of the connecting post is set to 5mm, the connecting post is set to a conical structure, and the inner diameter of the connecting post is set to 2mm.

[0010] Furthermore, the transition column is configured as a cone, and the inclination angle of the sidewall of the transition column is set to 45°.

[0011] Furthermore, the inner wall of the mounting column is configured as a slightly tapered hollow cylinder, and the diameter of the bottom end of the mounting column is set to 24.20 mm.

[0012] Furthermore, the inner top diameter of the mounting column is set to 24.50 mm, and the top of the mounting column is set as a flared, horn-shaped guide structure.

[0013] Furthermore, the sealing connection structure includes an inner extension platform, a limiting platform, and a sealing ring, wherein the inner extension platform is disposed at the connection between the top of the transition column and the mounting column.

[0014] Furthermore, the inner wall of the mounting column corresponding to the inner extension platform is recessed by 2.5mm, and a sealing ring is engaged in the space between the inner extension platform and the recessed inner wall of the mounting column.

[0015] Furthermore, the inner edge of the transition column top is provided with a protruding limiting platform, which is located inside the sealing ring and abuts against the inner wall of the sealing ring.

[0016] Furthermore, a flat-bottomed nucleic acid purification column body is connected inside the mounting column, and the bottom of the flat-bottomed nucleic acid purification column body abuts against the sealing ring and the top of the limiting platform.

[0017] The advantages of this application are:

[0018] 1. Negative Pressure Filtration Mode: Insert the flat-bottomed purification column into the mounting column of the adapter until the bottom of the purification column is tightly sealed with the sealing ring, then switch to negative pressure filtration mode, significantly improving operational efficiency; measured airtightness is greatly improved, with no leakage, and nucleic acid recovery rate ≥95%; High-Speed ​​Centrifugation Mode: Separate the adapter and purification column, then transfer the purification column to angle rotor centrifugation mode (12,000 rpm, 5 min); solution residue <20 μL, significantly better than centrifugation effect of pointed-bottom columns (residue >400 μL); one column for dual use, efficiently processing liquids under negative pressure filtration and extremely low solution residue under high-speed centrifugation; double sealing with silicone ring + conical seal, residue <20 μL; adapter cost ≈1 / 5 of purification column, and it is reusable.

[0019] 2. The sealing ring is installed in the inner extension platform at the connection between the transition column and the mounting column. The limiting platform at the edge of the inner extension platform abuts against the inner wall of the sealing ring, which can increase the stability of the sealing ring. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a cross-sectional structural diagram of one embodiment of this application;

[0022] Figure 2 This is a schematic diagram of the overall structure of one embodiment of this application;

[0023] Figure 3 This is a schematic diagram of the connection structure between a flat-bottomed nucleic acid purification column and an adapter according to an embodiment of this application;

[0024] Figure 4 This is one embodiment of the present application. Figure 3 A magnified structural diagram at point A;

[0025] Figure 5 This is a schematic diagram of the overall structure of the sealing ring according to one embodiment of this application.

[0026] In the diagram: 1. Connecting column; 2. Transition column; 3. Mounting column; 4. Sealing connection structure; 401. Inner extension platform; 402. Limiting platform; 403. Sealing ring; 5. Flat-bottomed nucleic acid purification column body. Detailed Implementation

[0027] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present application.

[0028] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0029] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0030] Furthermore, in addition to indicating location or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0031] Furthermore, the terms "installation," "setup," "equipped with," "connection," "linking," and "socketing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral structure; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0032] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0033] Please see Figure 1-5 As shown, a multifunctional nucleic acid purification column adapter includes a connecting column 1, a transition column 2 fixed to the top of the connecting column 1, and a mounting column 3 fixed to the top of the transition column 2. The multifunctional nucleic acid purification column adapter also includes:

[0034] A sealing connection structure 4 is provided at the connection between the transition column 2 and the mounting column 3.

[0035] The outer diameter of the bottom end of the connecting post 1 is set to 4.2 mm, and the outer diameter of the top end of the connecting post 1 is set to 4.5 mm.

[0036] The height of the connecting post 1 is set to 5mm, the connecting post 1 is set to a conical structure, and the internal diameter of the connecting post 1 is set to 2mm.

[0037] In this technical solution, the connecting column is made of medical-grade polypropylene (PP), and its corrosion resistance and biocompatibility meet the ISO 10993 standard; the conical structure (bottom Φ4.2mm → top Φ4.5mm) matches the standard Luer interface (Φ4.3mm), and the interface length is optimized to 8–10mm to enhance airtightness and torsional strength; a spiral guide groove is added to the inner wall to accelerate the flow of liquid through the pointed bottom hole (Φ2.0mm) and reduce residue.

[0038] The transition column 2 is set in a conical shape, and the inclination angle of the side wall of the transition column 2 is set to 45°.

[0039] In this technical solution, the 45° tilt angle design of the transition column is upgraded to a double curvature gradient flow channel (30° in the upper section → 60° in the lower section), which reduces fluid resistance and avoids bubble retention.

[0040] The inner wall of the mounting post 3 is set as a slightly tapered hollow cylinder, and the inner bottom diameter of the mounting post 3 is set to 24.20mm.

[0041] The inner top diameter of the mounting post 3 is set to 24.50 mm, and the top of the mounting post 3 is set as a flared funnel-shaped guide structure.

[0042] In this technical solution, the top of the mounting column 3 is set with an outward-curving flared guide structure to reduce insertion resistance and adapt to flat-bottomed columns of different brands.

[0043] The sealing connection structure 4 includes an inner extension platform 401, a limiting platform 402, and a sealing ring 403. The inner extension platform 401 is located at the connection between the top of the transition column 2 and the mounting column 3.

[0044] The inner wall of the mounting column 3 corresponding to the inner extension platform 401 is recessed by 2.5mm, and a sealing ring 403 is engaged in the space between the inner extension platform 401 and the recessed inner wall of the mounting column 3.

[0045] The inner extension platform 401 at the top of the transition column 2 is provided with a protruding limiting platform 402, which is located inside the sealing ring 403 and abuts against the inner wall of the sealing ring 403.

[0046] In this technical solution, the width of the inner extension platform is increased to 2.5mm, and a 1.5ml diameter annular silicone sealing ring 403 is added to double the sealing performance; a 0.5mm recessed limiting platform 402 is designed at the edge of the platform, which is lower than the height of the sealing ring 403 after compression, to prevent the sealing ring 403 from being over-compressed and deformed, thus losing its sealing performance.

[0047] The mounting column 3 is internally connected to a flat-bottomed nucleic acid purification column body 5, the bottom of which abuts against the sealing ring 403 and the top of the limiting platform 402.

[0048] Working principle and usage: First, the sealing ring 403 is installed in the inner extension platform 401 at the connection between the transition column 2 and the mounting column 3. The limiting platform 402 at the edge of the inner extension platform 401 abuts against the inner wall of the sealing ring 403, which can increase the stability of the sealing ring 403. Negative pressure filtration mode: Insert the flat-bottomed purification column into the mounting column 3 of the adapter until the bottom of the purification column presses against the sealing ring 403, and switch to negative pressure filtration mode, which greatly improves the operating efficiency. The measured airtightness is greatly improved, with no air leakage, and the nucleic acid recovery rate is ≥95%. High-speed centrifugation mode: Separate the adapter and the purification column, and transfer the purification column to the angle rotor centrifugation mode (12,000 rpm, 5 min). The solution residue is <20 μL, which is significantly better than the centrifugation effect of the pointed bottom column (residue >400 μL). The conical structure in column 1 matches the standard Luer interface, and the 245° tilt angle design of the transition column is upgraded to a double-curvature gradient flow channel to reduce fluid resistance and avoid air bubble retention. The width of the inner extension platform is increased to 2.5mm, and a 1.5ml diameter annular silicone sealing ring 403 is added to double ensure sealing performance. A 0.5mm recessed limiting platform 402 is designed at the edge of the platform, lower than the height of the sealing ring 403 after compression, to prevent the sealing ring 403 from being over-compressed and deformed, thus losing its sealing performance. One column has two uses: efficient liquid treatment under negative pressure filtration and extremely low solution residue under high-speed centrifugation. The silicone ring + conical double seal ensures a residue of <20μL. The adapter cost is approximately 1 / 5 of the purification column and it is reusable. The top of the mounting column 3 is designed with an outward-curving flared funnel-shaped guide structure to reduce insertion resistance and adapt to different brands of flat-bottom columns.

[0049] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A multifunctional nucleic acid purification column adapter, comprising a connecting column (1), a transition column (2) fixed to the top of the connecting column (1), and an mounting column (3) fixed to the top of the transition column (2), characterized in that, The multifunctional nucleic acid purification column adapter also includes: A sealing connection structure (4) is provided at the connection between the transition column (2) and the mounting column (3).

2. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The outer diameter of the bottom end of the connecting post (1) is set to 4.2 mm, and the outer diameter of the top end of the connecting post (1) is set to 4.5 mm.

3. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The height of the connecting post (1) is set to 5mm, the connecting post (1) is set to a conical structure, and the internal diameter of the connecting post (1) is set to 2mm.

4. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The transition column (2) is set in a conical shape, and the inclination angle of the side wall of the transition column (2) is set to 45°.

5. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The inner wall of the mounting column (3) is set as a slightly tapered hollow cylinder, and the inner bottom diameter of the mounting column (3) is set to 24.20 mm.

6. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The inner top diameter of the mounting post (3) is set to 24.50 mm, and the top of the mounting post (3) is set to a flared, horn-shaped guide structure.

7. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The sealing connection structure (4) includes an inner extension platform (401), a limiting platform (402), and a sealing ring (403). The inner extension platform (401) is located at the connection between the top of the transition column (2) and the mounting column (3).

8. The multifunctional nucleic acid purification column adapter according to claim 7, characterized in that: The inner wall of the mounting column (3) corresponding to the inner extension platform (401) is recessed by 2.5mm, and a sealing ring (403) is engaged in the space between the inner extension platform (401) and the recessed inner wall of the mounting column (3).

9. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The inner extension platform (401) at the top of the transition column (2) is provided with a protruding limiting platform (402) at its edge. The limiting platform (402) is located inside the sealing ring (403) and abuts against the inner wall of the sealing ring (403).

10. The multifunctional nucleic acid purification column adapter according to claim 1, characterized in that: The mounting column (3) is connected to a flat-bottomed nucleic acid purification column body (5), and the bottom of the flat-bottomed nucleic acid purification column body (5) abuts against the top of the sealing ring (403) and the limiting platform (402).

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