Automatic interception and identification device for microorganism in liquid

Abstract

The utility model discloses a kind of automatic interception identification devices of microorganism in liquid, including the detection mechanism for detecting microorganism in fluid, the sample collection mechanism for collecting microorganism and for culture, the power mechanism for liquid flow, still including control liquid flow direction, the control valve for intercepting the microorganism of flowing through detection mechanism into sample collection mechanism, the inlet end of the detection mechanism connection control valve, the power mechanism and sample collection mechanism are all connected the outlet end of control valve.The utility model is simple in structure, easy to operate, can automatically intercept microorganism, and identification accuracy is high.

Description

Technical Field

[0001] This utility model relates to the field of microbial identification technology, and in particular to an automatic interception and identification device for microorganisms in liquids. Background Technology

[0002] Microbial identification is a complex and meticulous task involving multiple steps and techniques. Its diversity and complexity are significant; over 100,000 species of microorganisms are known, with new species constantly being discovered. Microorganisms exhibit diverse morphological, physiological, and biochemical characteristics, and even the same microorganism may display different characteristics under different environmental conditions. This diversity and complexity makes screening and identification extremely difficult. When identifying microorganisms, it is essential to ensure that the sample is a pure culture, containing only one type of microorganism. If other microorganisms are present in the sample, the accuracy of the identification results will be affected.

[0003] The samples need to be representative and reflect the true characteristics of the target microorganism. This requires strict operating procedures when collecting and processing samples. Traditional identification methods, such as physiological and biochemical identification, Gram staining, and streak plating, are cumbersome, time-consuming, and highly dependent on the operator's experience and skill. Existing methods, such as molecular biology methods (PCR, sequencing, etc.), while offering higher accuracy and efficiency, have higher equipment costs and more demanding technical requirements. Any errors in the experimental process can lead to deviations in the identification results, such as contamination and cross-contamination. Traditional microbial identification requires microbial culture, purification, and then further culture for PCR, sequencing, etc. The culture and purification process before identification takes a considerable amount of time and may need to be repeated to obtain pure microorganisms for identification. Utility Model Content

[0004] To address the problems existing in the prior art, this invention provides an automatic microbial retention and identification device in liquids. This device automatically extracts microorganisms, accurately and quickly extracts and purifies them, saving time and effort, and providing higher accuracy in identification.

[0005] The technical solution adopted is as follows:

[0006] An automatic microbial retention and identification device in liquid includes a detection mechanism for detecting microorganisms in a fluid, a sample collection mechanism for collecting microorganisms and culturing them, a power mechanism for causing the liquid to flow, and a control valve for controlling the direction of liquid flow and retaining microorganisms flowing through the detection mechanism from entering the sample collection mechanism. The detection mechanism is connected to the inlet end of the control valve, and the power mechanism and the sample collection mechanism are both connected to the outlet end of the control valve.

[0007] Furthermore, the control valve is a two-position three-way solenoid valve.

[0008] Furthermore, the normally open end of the control valve is connected to the power mechanism, and the normally closed end is connected to the sample collection mechanism.

[0009] Furthermore, the detection mechanism includes a laser, a flow chamber through which the water to be tested flows, a photodetector, and a photomultiplier tube. A spot shaping system, a flow chamber, a scattered light collection system, and a photodetector are arranged sequentially in the direction of propagation of the laser emitted light. The spot shaping system shapes the laser emitted light spot into a linear spot that illuminates the flow chamber. The scattered light collection system collects the scattered light and focuses it onto the receiving surface of the photodetector. A fluorescence capture system is provided on one side of the flow chamber, and a reflector is provided on the other side to reflect the fluorescence to the opposite side.

[0010] Furthermore, the flow chamber is provided with an inlet pipe and an outlet pipe at both ends, and the outlet pipe is connected to a control valve.

[0011] Furthermore, the power mechanism is a pump that provides negative pressure for drawing liquid.

[0012] Furthermore, the pump body is a gear pump.

[0013] Furthermore, the sample collection mechanism is a vacuum environment, and a culture medium is provided inside the sample collection mechanism.

[0014] Furthermore, the pump body includes a pump body inlet pipe and a pump body outlet pipe, and the pump body inlet pipe is connected to a control valve.

[0015] Furthermore, the sample collection mechanism is connected to a control valve via a collection tube.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] This invention provides an automatic microbial retention and identification device for liquids. The liquid to be tested flows through a detection mechanism to detect whether it contains microorganisms. The detection mechanism is connected to the inlet of a control valve, while the sample collection mechanism and power mechanism are both connected to the outlet of the control valve. The power mechanism is connected to the normally open end, and the sample collection mechanism is connected to the normally closed end. The power mechanism can be a pump to generate negative pressure on the liquid and extract it. When the control valve is not open, the power mechanism remains open to keep the liquid flowing. If microorganisms are detected by the detection mechanism, the control valve is opened after a delay according to the pipeline flow rate, the power mechanism is closed, and the sample collection mechanism is opened. Under the negative pressure of the collection tube, the liquid containing microorganisms is retained in the sample collection mechanism. The entire process is automatically achieved through pipeline switching via the control valve, avoiding contact with the outside environment and reducing external contamination. The microorganisms entering the collection tube have higher purity. Furthermore, the sample collection mechanism contains a culture medium for direct microbial cultivation without opening the collection tube during cultivation, thus avoiding contact with the outside environment. Only one cultivation is required, without purification or secondary cultivation. The operation is simple and saves time. This invention has a simple structure and uses an active switching control valve to avoid contact with the outside world, resulting in higher accuracy of subsequent evaluation results. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a side view of the present invention;

[0020] Figure 3 This is a schematic diagram of the internal structure of the testing facility;

[0021] The components include: 1. Detection mechanism; 2. Sample collection mechanism; 3. Power mechanism; 4. Control valve; 5. Laser; 6. Flow chamber; 601 sample inlet tube; 602 sample outlet tube; 7. Photodetector; 8. Photomultiplier tube; 9. Spot shaping system; 10. Scattered light collection system; 11. Fluorescence capture system; 12. Reflector; 13. Central block; 14. Pump body; 1401 pump body inlet tube; 1402 pump body outlet tube; 15. Collection tube; 16. Front light-blocking plate; 17. First mounting base; 18. Second mounting base; and 19. Third mounting base. Detailed Implementation

[0022] The present invention will be further described below with reference to specific embodiments.

[0023] refer to Figure 1 , 23. An automatic microbial interception and identification device in a liquid includes a detection mechanism 1 for detecting microorganisms in a fluid, a sample collection mechanism 2 for collecting microorganisms and culturing them, a power mechanism 3 for causing the liquid to flow, and a control valve 4 for controlling the direction of liquid flow and intercepting microorganisms flowing through the detection mechanism from entering the sample collection mechanism. The detection mechanism 1 is connected to the inlet end of the control valve 4, and the power mechanism 3 and the sample collection mechanism 2 are both connected to the outlet end of the control valve 4.

[0024] The control valve 4 is a two-position three-way solenoid valve. The normally open end of the control valve 4 is connected to the power mechanism 3, and the normally closed end is connected to the sample collection mechanism 2. When the control valve is not open, the sample collection mechanism 2 is normally closed, and the power mechanism 3 is normally open. The power mechanism 3 is a pump body, such as a gear pump, that provides negative pressure for extracting liquid. The pump body includes a pump body inlet pipe 1401 and a pump body outlet pipe 1402. The pump body inlet pipe is connected to the control valve 4, and the pump body outlet pipe 1402 can discharge liquid outside the testing system. When the sampling system is running, the pump body 1401 creates a negative pressure at the pump body inlet end, drawing in liquid and causing it to flow within the detection mechanism 1.

[0025] The sample collection mechanism 2 has a vacuum environment inside and contains a complete culture medium. The sample collection mechanism 2 is connected to the control valve 4 via a collection tube 15.

[0026] The detection mechanism 1 can fully utilize existing technology, employing laser-induced fluorescence technology and the principle of Mie scattering to detect whether particles pass through the detection area and parameters such as particle diameter. The detection mechanism 1 includes a laser 5, a flow chamber 6 through which the water to be tested flows, a photodetector 7, and a photomultiplier tube 8. Along the propagation direction of the laser light emitted, a spot shaping system 9, the flow chamber 6, a scattered light collection system 10, and the photodetector 7 are sequentially arranged. The spot shaping system 9 shapes the laser light emitted from the laser 5 into a linear spot that illuminates the flow chamber 6. The scattered light collection system 10 collects the scattered light and focuses it onto the receiving surface of the photodetector 7. A fluorescence capture system 11 is located on one side of the flow chamber 6, and a reflector 12 is located on the other side to reflect the fluorescence to the opposite side. An inlet pipe 601 and an outlet pipe 602 are located at both ends of the flow chamber 6, with the outlet pipe 602 connected to a control valve 4.

[0027] The liquid to be tested flows in through the sample inlet tube 601 and passes through the flow chamber 6. The laser 5 emits a low-power laser beam, which is then shaped into a nanoscale spot at the center of the flow chamber 6 by the spot shaping system 9. The scattered light collection system 10 has a front light-blocking plate 16 near one end of the flow chamber to block the path of the light. The scattered light collection system 10 is used to collect the scattered light. When a particle passes through the detection area, due to the principle of particle scattering, some of the scattered light cannot be blocked by the front light-blocking plate 16. The scattered light collection system 10 focuses the collected scattered light onto the photodetector 7, which is used to receive changes in the light signal. Thus, by combining the changes in light energy with the liquid flow rate, parameters such as whether a particle has passed through the detection area and the particle diameter are determined.

[0028] The flow chamber 6 is mounted on the central block 13, the fluorescence capture system 11 is mounted on the first mounting base 17, the scattered light collection system 10 and the photodetector 7 are mounted on the second mounting base 18, and the laser 5 and the spot shaping system 9 are mounted on the third mounting base 19. The first, second, and third mounting bases are respectively connected to the central block 13.

[0029] Microorganisms in the water system are irradiated by a shaped linear light spot, emitting fluorescence in all directions. A fluorescence capture system 11 is located on one side of the flow chamber, while a reflector 12 on the other side reflects the fluorescence back to the opposite side. The reflector 12 focuses the fluorescence onto the side with the fluorescence capture system 11, where it is received by the collecting lens of the fluorescence capture system at a wide angle and converged onto the photomultiplier tube 8. The photomultiplier tube 8 collects and detects changes in fluorescence signals to determine whether particles passing through the detection area are microorganisms.

[0030] The liquid continues to flow. When it is determined that microorganisms have passed through the detection area, the control valve 4 is opened with a delay according to the pipeline flow rate. After the control valve is opened, the pump body 14 connected to the normally open end is closed, and the sample collection mechanism 2 connected to the normally closed end is connected. Since the sample collection mechanism 2 is a vacuum environment, it acts as a negative pressure power source to draw the liquid in the pipeline into the sample collection mechanism 2, thus completing one microbial interception operation.

[0031] Sample collection unit 2 is equipped with pre-prepared culture medium. Microorganisms in the sample are captured within sample collection unit 2 and then transferred to an incubator for cultivation. The resulting bacterial strains are then identified. Microbial identification can be performed in a single cultivation session, eliminating the need for secondary purification and cultivation. The entire collection process is fully enclosed, preventing external microbial interference and resulting in higher microbial purity. Real-time collection of single microorganisms and fully automated extraction allow for precise and rapid capture and purification, leading to higher identification accuracy.

[0032] The above description is only an optional embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.

Claims

1. An automated device for the retention and identification of microorganisms in liquid, characterized in that: It includes a detection mechanism (1) for detecting microorganisms in a fluid, a sample collection mechanism (2) for collecting microorganisms and culturing them, a power mechanism (3) for making the liquid flow, and a control valve (4) for controlling the direction of liquid flow and for intercepting microorganisms flowing through the detection mechanism from entering the sample collection mechanism. The detection mechanism (1) is connected to the inlet end of the control valve (4), and the power mechanism (3) and the sample collection mechanism (2) are both connected to the outlet end of the control valve (4).

2. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The control valve (4) is a two-position three-way solenoid valve.

3. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The normally open end of the control valve (4) is connected to the power mechanism (3), and the normally closed end is connected to the sample collection mechanism (2).

4. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The detection mechanism (1) includes a laser (5), a flow chamber (6) through which the water to be tested flows, a photodetector (7) and a photomultiplier tube (8). A spot shaping system (9), a flow chamber (6), a scattered light collection system (10) and a photodetector (7) are arranged in sequence along the propagation direction of the light emitted by the laser (5). The spot shaping system (9) shapes the light emitted by the laser (5) into a linear spot that illuminates the flow chamber (6). The scattered light collection system (10) collects the scattered light and focuses it onto the receiving surface of the photodetector (7). A fluorescence capture system (11) is provided on one side of the flow chamber (6), and a reflector (12) is provided on the other side to reflect the fluorescence to the opposite side.

5. The automatic retention and identification device for microorganisms in liquids as described in claim 4, characterized in that: The flow chamber (6) is provided with an inlet pipe (601) and an outlet pipe (602) at both ends, and the outlet pipe (602) is connected to a control valve (4).

6. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The power mechanism (3) is a pump body (14) that provides negative pressure for drawing liquid.

7. The automatic retention and identification device for microorganisms in liquids as described in claim 6, characterized in that: The pump body (14) is a gear pump.

8. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The sample collection mechanism (2) is a vacuum environment inside, and a culture medium is provided inside the sample collection mechanism (2).

9. The automatic retention and identification device for microorganisms in liquids as described in claim 6, characterized in that: The pump body (14) includes a pump body inlet pipe (1401) and a pump body outlet pipe (1402), and the pump body inlet pipe is connected to a control valve (4).

10. The automatic retention and identification device for microorganisms in liquids as described in claim 1, characterized in that: The sample collection mechanism (2) is connected to the control valve (4) through the collection tube (15).

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