A device for purifying samples of caenorhabditis elegans based on flushing and centrifugation
By designing components such as scrapers and guide rings, the Caenorhabditis elegans sample rinsing and centrifugation purification device solves the problems of nematode blockage and contamination, achieving efficient purification and cleaning operations.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- FUJIAN SUNYBIOTECH CO LTD
- Filing Date
- 2025-07-23
- Publication Date
- 2026-06-26
AI Technical Summary
In existing technologies, Caenorhabditis elegans tends to stick to the sieve during centrifugation and filtration, causing blockages, and the buffer solution and impurities splash and contaminate the laboratory environment.
A device comprising a motor, a turntable, a filter assembly, a sealing ring, a retaining ring, a guide ring, and a scraper is designed. The scraper removes adhering nematodes, the guide ring collects splashed liquid, and the sealing ring prevents splashing, thus avoiding blockage and contamination.
It effectively prevents Caenorhabditis elegans from sticking to the filter screen, reduces laboratory contamination, improves filtration efficiency, and facilitates material removal.
Smart Images
Figure CN224405373U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of Caenorhabditis elegans sample washing, specifically a Caenorhabditis elegans sample washing and centrifugation purification device. Background Technology
[0002] *C. elegans* is an internationally recognized model organism with advantages in anti-aging research. It boasts simple experimental procedures, a short developmental cycle, clearly defined genetic information, and ease of large-scale culture, making it widely used in scientific research related to anti-aging, neurobehavioral science, environmental science, and food science. Furthermore, *C. elegans* was the first eukaryote to have its entire genome discovered, with over 40% of its genes homologous to human genes. The discovery of its complete genome sequence provides abundant research resources for genetics, laying the foundation for studying the genetic mechanisms of human aging through nematodes. In experimental biology, *C. elegans* samples are frequently washed, centrifuged, and purified, primarily for isolating and purifying nematodes from culture systems and removing impurities such as bacteria, eggs, and molted worms.
[0003] In existing techniques for rinsing and centrifuging samples of Caenorhabditis elegans, the nematodes and impurities move towards the sieve along with the centrifugal force. The nematodes can stick to the sieve, causing blockage and affecting the filtration effect. Furthermore, when centrifuging in the laboratory, buffer solution needs to be added to the centrifuge. As the centrifuge is turned on, the buffer solution and the filtered impurities splash around, contaminating the laboratory environment.
[0004] Therefore, this invention provides a sample rinsing and centrifugation purification device based on Caenorhabditis elegans. Utility Model Content
[0005] To overcome the shortcomings of existing technologies and address the issues arising from the following: during the washing and centrifugation filtration of *C. elegans* samples, the existing technology causes *C. elegans* and impurities to move towards the sieve along with the centrifuge due to centrifugal force. The *C. elegans* may stick to the sieve, causing blockage and affecting filtration efficiency. Furthermore, in laboratory centrifugation, buffer solution needs to be added to the centrifuge, which can cause the buffer solution and filtered impurities to splash and contaminate the laboratory environment. Therefore, this invention proposes a *C. elegans* sample washing and centrifugation purification device.
[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: The present utility model provides a sample rinsing and centrifugation purification device based on Caenorhabditis elegans, including a base. A motor is fixedly connected to the top of the base, and a turntable is fixedly connected to the output end of the motor. A filter assembly is arranged above the turntable. Two side plates are symmetrically fixedly connected to the top of the base. A fixing plate is fixedly connected to the outer wall of each of the two side plates. An electric telescopic rod is fixedly connected to the top of each of the two fixing plates. A funnel is fixedly connected to the output end of each of the two electric telescopic rods, and a water outlet pipe is fixedly connected to the bottom of the funnel.
[0007] Preferably, the filter assembly includes a fixing ring disposed above the turntable. The fixing ring has several outlets equidistantly arranged inside, and a filter screen is disposed inside each outlet. Several locking blocks are fixedly connected at equal intervals at the bottom of the fixing ring, and several slots are equidistantly arranged at the top of the turntable. The locking blocks and slots are used in conjunction.
[0008] Preferably, a sealing ring is fixedly connected to the bottom of the fixing ring, and a sealing groove is provided on the top of the turntable, with the sealing ring fitting against the inner wall of the sealing groove.
[0009] Preferably, a retaining ring is fixedly connected to the inner wall of the fixing ring, and the inner wall of the retaining ring is configured as an annular inclined surface.
[0010] Preferably, a guide ring is fixedly connected to the top of the fixing ring, and the inner wall of the guide ring is set as an annular arc surface.
[0011] Preferably, a water storage tank is provided on the top of the base and below the guide ring.
[0012] Preferably, a plurality of supports are fixedly connected at equal intervals to the outer wall of the water outlet pipe, a scraper is fixedly connected to one end of the support, the scraper is in contact with the water outlet, the outer wall of the scraper is set as a symmetrical inclined surface, a flow groove is opened inside the support, and a water outlet groove is opened at the bottom of the support, and the water outlet groove communicates with the flow groove.
[0013] Preferably, a ring frame is fixedly connected to the top of the base, and a support ring is fixedly connected to the top of the ring frame, with the support ring fitting against the bottom of the turntable.
[0014] The beneficial effects of this utility model are as follows:
[0015] 1. The present invention relates to a centrifugal purification device for rinsing samples of *C. elegans*. When the turntable and the fixed ring rotate, the scraper rotates against the inner wall of the fixed ring. The scraper scrapes off the *C. elegans* adhering to the filter screen, preventing the *C. elegans* from clogging the filter screen. The scraped *C. elegans* moves along the inclined surface of the scraper towards the center of the turntable, thereby moving the scraped *C. elegans* away from the filter screen. During centrifugation and filtration, buffer solution is added to the funnel. The buffer solution enters the outlet pipe and flows along the flow channel, exiting from the outlet channel to rinse the filter screen and prevent the *C. elegans* from sticking to the filter screen.
[0016] 2. The present invention provides a sample rinsing and centrifugation purification device based on *C. elegans*. A baffle ring blocks the top of the fixed ring, preventing *C. elegans* and filtered impurities from escaping from the top of the fixed ring. A guide ring blocks buffer solution and impurities escaping from the outlet, preventing contamination of the laboratory environment. The escaped buffer solution and impurities flow downwards along the inner wall of the guide ring and are collected and processed through a water tank.
[0017] 3. The present invention provides a sample washing and centrifugation purification device for Caenorhabditis elegans. After the centrifugation purification of Caenorhabditis elegans is completed, the funnel is moved upward by an electric telescopic rod. Through the cooperation of the support and the retaining ring, the fixed ring is moved upward and separated from the turntable. After the fixed ring is separated from the turntable, it is convenient to take the centrifuged and purified Caenorhabditis elegans sample. Attached Figure Description
[0018] Figure 1 This is a perspective view of the present invention;
[0019] Figure 2 This is a cross-sectional view of the present invention;
[0020] Figure 3 This is an exploded view of the turntable and fixing ring used in conjunction with this utility model;
[0021] Figure 4 This is a perspective view of the scraper and water outlet channel of this utility model in use.
[0022] Figure 5 This is a utility model Figure 2 Enlarged view of point B in the middle;
[0023] In the diagram: 1. Base; 2. Motor; 3. Turntable; 4. Fixing plate; 5. Electric telescopic rod; 6. Funnel; 7. Fixing ring; 8. Sealing ring; 9. Sealing groove; 10. Locking block; 11. Locking groove; 12. Outlet; 13. Filter screen; 14. Baffle ring; 15. Guide ring; 16. Outlet pipe; 17. Bracket; 18. Scraper; 19. Flow channel; 20. Outlet channel; 21. Water storage tank; 22. Ring frame; 23. Support ring; 24. Side plate. Detailed Implementation
[0024] To illustrate the possible application scenarios, technical principles, implementable specific solutions, and achievable objectives and effects of this application in detail, the following description, in conjunction with the listed specific embodiments and accompanying drawings, provides a detailed explanation. The embodiments described herein are merely illustrative of the technical solutions of this application and are therefore intended to limit the scope of protection of this application.
[0025] In this document, the term "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The term "embodiment" appearing in various places throughout the specification does not necessarily refer to the same embodiment, nor does it specifically limit its independence or connection with other embodiments. In principle, in this application, as long as there are no technical contradictions or conflicts, the technical features mentioned in each embodiment can be combined in any way to form corresponding implementable technical solutions.
[0026] Unless otherwise defined, the technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the use of related terms herein is merely for the purpose of describing particular embodiments and is not intended to limit this application.
[0027] In the description of this application, the term "and / or" is used to describe the logical relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A exists, B exists, and A and B exist simultaneously. Additionally, the character " / " in this document generally indicates that the preceding and following objects have an "or" logical relationship.
[0028] In this application, terms such as “first” and “second” are used only to distinguish one entity or operation from another, and do not necessarily require or imply any actual quantity, hierarchy or order relationship between these entities or operations.
[0029] Without further limitations, the use of terms such as “comprising,” “including,” “having,” or other similar open-ended expressions in this application is intended to cover non-exclusive inclusion, which does not exclude the presence of additional elements in a process, method, or product that includes the stated elements, such that a process, method, or product that includes a list of elements may include not only those defined elements but also other elements not expressly listed, or elements inherent to such a process, method, or product.
[0030] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than," "less than," and "exceeding" are understood to exclude the stated number; expressions such as "above," "below," and "within" are understood to include the stated number. Furthermore, in the description of the embodiments in this application, "multiple" means two or more (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups" and "multiple times," unless otherwise explicitly specified.
[0031] In the description of the embodiments of this application, the space-related expressions used, such as "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "vertical," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," indicate the orientation or positional relationship based on the orientation or positional relationship shown in the specific embodiments or drawings. They are only for the purpose of describing the specific embodiments of this application or for the reader's understanding, and do not indicate or imply that the device or component referred to must have a specific position, a specific orientation, or be constructed or operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.
[0032] Unless otherwise expressly specified or limited, the terms "installation," "connection," "linking," "fixing," and "setting," as used in the description of the embodiments of this application, should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral arrangement; it can be a direct connection or an indirect connection through an intermediate medium; it can be a relationship of two components combined together, an interaction relationship between two components, or a connection within two structures. Those skilled in the art to which this application pertains can understand the specific meaning of the above terms in the embodiments of this application according to the specific circumstances.
[0033] like Figures 1 to 5As shown, this utility model provides a technical solution: a sample rinsing and centrifugation purification device based on *C. elegans*, including a base 1. A motor 2 is fixedly connected to the top of the base 1, and a turntable 3 is fixedly connected to the output end of the motor 2. A filter assembly is arranged above the turntable 3. Two side plates 24 are symmetrically fixedly connected to the top of the base 1. Fixing plates 4 are fixedly connected to the outer walls of both side plates 24. Electric telescopic rods 5 are fixedly connected to the top of both fixing plates 4. Funnels 6 are fixedly connected to the output ends of the two electric telescopic rods 5. The bottom of the funnels 6... A water outlet pipe 16 is fixedly connected. The filter assembly includes a fixing ring 7, which is located above the turntable 3. Several water outlets 12 are equidistantly arranged inside the fixing ring 7. A filter screen 13 is arranged inside the water outlet 12. Several locking blocks 10 are fixedly connected at equal intervals at the bottom of the fixing ring 7. Several slots 11 are equidistantly arranged at the top of the turntable 3. The locking blocks 10 and slots 11 are used in conjunction. A ring frame 22 is fixedly connected to the top of the base 1. A support ring 23 is fixedly connected to the top of the ring frame 22. The support ring 23 fits against the bottom of the turntable 3.
[0034] Using the above technical solution, the *C. elegans* sample is placed on the turntable 3, and the fixing ring 7 is aligned with the turntable 3. The locking block 10 is inserted into the locking slot 11. Buffer solution is filled into the funnel 6, and the buffer solution enters the turntable 3 along the outlet pipe 16 to soak the *C. elegans* sample. The motor 2 is started, driving the turntable 3 to rotate. Through the cooperation of the locking block 10 and the locking slot 11, the turntable 3 rotates while driving the fixing ring 7 to rotate, thereby generating centrifugal force to filter the *C. elegans* sample and the buffer solution. The buffer solution, mixed with impurities in the *C. elegans* sample, is discharged from the filter screen 13, and the remaining *C. elegans* remains on the turntable 3. While the turntable 3 and the fixing ring 7 are rotating, the bottom of the turntable 3 is supported by the support ring 23 to maintain the stability of the turntable 3.
[0035] Specifically, a sealing ring 8 is fixedly connected to the bottom of the fixed ring 7, and a sealing groove 9 is opened on the top of the turntable 3, with the sealing ring 8 fitting against the inner wall of the sealing groove 9.
[0036] With the above technical solution, when the fixing ring 7 is connected to the turntable 3, the sealing ring 8 is inserted into the sealing groove 9. Through the cooperation of the sealing ring 8 and the sealing groove 9, the connection between the fixing ring 7 and the turntable 3 is sealed to prevent liquid leakage.
[0037] Specifically, a retaining ring 14 is fixedly connected to the inner wall of the fixing ring 7, and the inner wall of the retaining ring 14 is set as an annular inclined surface.
[0038] With the above technical solution, when the turntable 3 and the fixed ring 7 rotate, the upper part of the fixed ring 7 is blocked by the set baffle ring 14 to prevent the Caenorhabditis elegans and the filtered impurities from flying out from the upper part of the fixed ring 7.
[0039] Specifically, a guide ring 15 is fixedly connected to the top of the fixed ring 7. The inner wall of the guide ring 15 is set as an annular arc surface. A water storage tank 21 is provided on the top of the base 1 and below the guide ring 15.
[0040] Through the above technical solution, the guide ring 15 blocks the buffer solution and impurities flying out of the outlet 12, avoiding contamination of the laboratory environment by the buffer solution and impurities. The flying buffer solution and impurities flow downward along the inner wall of the guide ring 15 and are collected and treated by the water storage tank 21.
[0041] Specifically, several supports 17 are fixedly connected at equal intervals on the outer wall of the water outlet pipe 16. A scraper 18 is fixedly connected to one end of the support 17. The scraper 18 fits the water outlet 12. The outer wall of the scraper 18 is set as a symmetrical inclined surface. A flow groove 19 is opened inside the support 17. A water outlet groove 20 is opened at the bottom of the support 17. The water outlet groove 20 is connected to the flow groove 19.
[0042] With the above technical solution, when the turntable 3 and the fixed ring 7 rotate, the scraper 18 rotates against the inner wall of the fixed ring 7. The scraper 18 scrapes off the Caenorhabditis elegans adhering to the filter screen 13, preventing the Caenorhabditis elegans from clogging the filter screen 13. The scraped-off Caenorhabditis elegans moves along the slope of the scraper 18 towards the center of the turntable 3, thus moving the scraped-off Caenorhabditis elegans away from the filter screen 13. At the same time as centrifugal filtration, buffer solution is added to the funnel 6, and the buffer solution enters the outlet pipe 1. After 6, the water flows along the flow channel 19 and is discharged from the outlet channel 20, rinsing the filter screen 13 to prevent the Caenorhabditis elegans from sticking to the filter screen 13. After the centrifugation purification of the Caenorhabditis elegans is completed, the electric telescopic rod 5 is started to drive the funnel 6 to move upward, so that the support 17 moves upward. The support 17 presses against the bottom of the retaining ring 14, pushing the retaining ring 14 to move upward, so that the fixing ring 7 moves upward and separates from the turntable 3. After the fixing ring 7 is separated from the turntable 3, it is convenient to take the centrifuged purified Caenorhabditis elegans.
[0043] In use, place the *C. elegans* sample on the turntable 3 and align the fixing ring 7 with the turntable 3. Insert the locking block 10 into the locking groove 11. When the fixing ring 7 is aligned with the turntable 3, the sealing ring 8 is inserted into the sealing groove 9. The cooperation between the sealing ring 8 and the sealing groove 9 seals the connection between the fixing ring 7 and the turntable 3, preventing liquid leakage. Fill the funnel 6 with buffer solution, and the buffer solution enters the turntable 3 along the outlet pipe 16 to soak the *C. elegans* sample. Start the motor 2 to rotate the turntable 3. Through the cooperation between the locking block 10 and the locking groove 11, the turntable 3... The rotation of the rotating disc 3 causes the fixed ring 7 to rotate, thereby generating centrifugal force to filter the *C. elegans* sample and buffer solution. The buffer solution, mixed with impurities from the *C. elegans* sample, is discharged through the filter screen 13, while the remaining *C. elegans* remains on the rotating disc 3. As the rotating disc 3 and the fixed ring 7 rotate, the top of the fixed ring 7 is blocked by the baffle ring 14 to prevent *C. elegans* and filtered impurities from flying out from the top of the fixed ring 7. The guide ring 15 blocks the buffer solution and impurities flying out from the outlet 12, preventing the buffer solution and impurities from contaminating the laboratory. In the environment, the ejected buffer solution and impurities flow downwards along the inner wall of the guide ring 15, and are collected and processed by the water storage tank 21. When the turntable 3 and the fixed ring 7 rotate, the scraper 18 rotates against the inner wall of the fixed ring 7. The scraper 18 scrapes off the Caenorhabditis elegans adhering to the filter screen 13, preventing the Caenorhabditis elegans from clogging the filter screen 13. The scraped-off Caenorhabditis elegans moves along the slope of the scraper 18 towards the center of the turntable 3, thus moving the scraped-off Caenorhabditis elegans away from the filter screen 13, and during centrifugal filtration, it is collected in the funnel. 6. Buffer solution is added to the water outlet pipe 16 and flows along the flow channel 19, exiting from the water outlet channel 20 to rinse the filter screen 13, preventing Caenorhabditis elegans from sticking to the filter screen 13. After the centrifugation purification of Caenorhabditis elegans is completed, the electric telescopic rod 5 is activated to move the funnel 6 upward, causing the support 17 to move upward. The support 17 presses against the bottom of the retaining ring 14, pushing the retaining ring 14 upward, causing the fixing ring 7 to move upward and separate from the turntable 3. After the fixing ring 7 is separated from the turntable 3, it is convenient to take the centrifuged and purified Caenorhabditis elegans.
[0044] Finally, it should be noted that although the above embodiments have been described in the text and drawings of this application, this should not limit the scope of patent protection of this application. Any technical solutions that are based on the essential concept of this application and utilize the content described in the text and drawings of this application, resulting in equivalent structural or procedural substitutions or modifications, as well as the direct or indirect application of the technical solutions of the above embodiments to other related technical fields, are all included within the scope of patent protection of this application.
Claims
1. A sample rinsing and centrifugation purification device based on *C. elegans*, characterized in that, The device includes a base, a motor fixedly connected to the top of the base, a turntable fixedly connected to the output end of the motor, a filter assembly above the turntable, two side plates symmetrically fixedly connected to the top of the base, a fixing plate fixedly connected to the outer wall of each side plate, an electric telescopic rod fixedly connected to the top of each fixing plate, a funnel fixedly connected to the output end of each electric telescopic rod, and a water outlet pipe fixedly connected to the bottom of the funnel.
2. The apparatus for rinsing, centrifuging, and purifying samples based on *C. elegans* according to claim 1, characterized in that, The filter assembly includes a fixing ring positioned above the turntable. The fixing ring has several outlets equidistantly arranged inside it, and a filter screen is installed inside each outlet. Several locking blocks are fixedly connected at equal intervals to the bottom of the fixing ring, and several slots are equidistantly arranged on the top of the turntable. The locking blocks and slots are used in conjunction.
3. The apparatus for rinsing and centrifuging purification of *C. elegans* samples according to claim 2, characterized in that, A sealing ring is fixedly connected to the bottom of the fixed ring, and a sealing groove is opened on the top of the turntable, with the sealing ring fitting against the inner wall of the sealing groove.
4. The apparatus for rinsing and centrifuging purification of *C. elegans* samples according to claim 2, characterized in that, A retaining ring is fixedly connected to the inner wall of the fixed ring, and the inner wall of the retaining ring is set as an annular inclined surface.
5. The apparatus for rinsing and centrifuging purification of *C. elegans* samples according to claim 2, characterized in that, A guide ring is fixedly connected to the top of the fixed ring, and the inner wall of the guide ring is set as an annular arc surface.
6. The Caenorhabditis elegans sample washing and centrifugation purification device according to claim 5, characterized in that, A water storage tank is provided on the top of the base and below the flow guide ring.
7. The apparatus for rinsing and centrifuging purification of *C. elegans* samples according to claim 2, characterized in that, The outer wall of the water outlet pipe is fixedly connected with several supports at equal intervals. A scraper is fixedly connected to one end of each support. The scraper fits the water outlet and the outer wall of the scraper is set as a symmetrical inclined surface. A flow groove is opened inside the support and a water outlet groove is opened at the bottom of the support. The water outlet groove is connected to the flow groove.
8. The apparatus for rinsing and centrifuging purification of *C. elegans* samples according to claim 1, characterized in that, A ring frame is fixedly connected to the top of the base, and a support ring is fixedly connected to the top of the ring frame. The support ring fits against the bottom of the turntable.