Embryo thawing culture dish

Abstract

The utility model discloses an embryo thawing culture dish, including dish body, one pair of sliding grooves is equipped with symmetry on dish body outside, is equipped with movable area and strip low groove inside, top still can be slidably equipped with dish cover, be equipped with movable dish in movable area, be equipped with three culture holes in strip low groove, dish cover includes two sliding plates, and the both sides of each sliding plate are equipped with the sliding block assembly of sliding cooperation with sliding groove, and the connecting surface of one sliding plate is equipped with recess, and the connecting surface of another sliding plate is equipped with the clamping block of corresponding recess, and clamping block and recess cooperate. The utility model discloses through setting up the dish cover structure of slidability, avoided the confusion risk of dish cover separation, improved the operation safety, and the design of movable dish and strip low groove realized the independent preheating and use of different temperature demand liquid, and strip groove is convenient for to three culture holes of adding mineral oil simultaneously, simplified the operation process, and the thawing effect was improved, and the whole stable structure has the advantage that is convenient, safe and reliable to operate.

Description

Technical Field

[0001] This utility model relates to the field of human assisted reproductive technology, specifically to an embryo thawing culture dish. Background Technology

[0002] With the rapid development of assisted reproductive technology and the increasing sophistication of controlled ovarian stimulation and embryo culture techniques, embryo cryopreservation and thawing has become an indispensable part of human assisted reproductive technology. This technique, through embryo cryopreservation and thawing, allows for the reasonable limitation of the number of embryos transferred, effectively reducing the rate of multiple pregnancies and increasing the cumulative live birth rate per ovarian stimulation cycle, while also alleviating costs for patients whose fresh cycle embryo transfer fails. For patients with a large number of retrieved eggs, whole-embryo cryopreservation facilitates subsequent use and reduces the stimulation and damage to the ovaries caused by repeated egg retrieval, thus alleviating the patient's treatment suffering.

[0003] For cryopreserved embryos, the following thawing methods were used: The first method used four-well petri dishes for thawing without covering with mineral oil; the second method used individual petri dishes, specifically 60mm petri dishes with self-made microdroplets and covered with mineral oil. Four-well petri dishes are more widely used because each well can be used independently, making them easier to handle. However, research shows that covering with mineral oil prevents liquid evaporation during thawing equilibrium, thus preventing osmotic pressure and cryoprotectant concentration. Therefore, covering with mineral oil results in better embryo thawing than not covering with mineral oil.

[0004] The current four-well culture dish design is inconvenient for adding mineral oil, and the pores are all fixed structures. Furthermore, one of the four liquids required for thawing needs incubation at 37 degrees Celsius, while the other three need incubation at room temperature before use. However, because the pores of the four-well culture dish are fixed, the liquids for high-temperature incubation need to be stored in separate containers, making the operation cumbersome. When using microdroplets for thawing, the droplets used in the culture dish are generally around 200 microliters in volume, which is large and easily affected by shaking, impacting the subsequent thawing effect. Also, operating multiple culture dishes simultaneously can easily lead to confusion. In addition, the bottom and lid of these culture dishes are detachable, which can easily cause medical accidents due to misplaced lids when transferring embryos from multiple individuals simultaneously. Based on these shortcomings, the existing culture dishes need to be improved. Utility Model Content

[0005] To address the shortcomings of existing culture dishes, such as the risk of medical accidents due to lid separation, the inconvenience of adding mineral oil to four-well culture dishes, and the tendency for microdroplets to shake, which can affect the thawing effect, this invention provides an embryo thawing culture dish that solves the problem of easy lid separation, facilitates the addition of mineral oil, and is less likely to affect the thawing effect due to shaking.

[0006] To achieve the above objectives, the technical solution of this utility model is as follows:

[0007] An embryo thawing culture dish includes a dish body with a pair of symmetrical sliding grooves on the outer side, an active area and a strip-shaped groove inside, and a dish lid that can be slidably mounted on the top. A movable dish is detachably mounted in the active area, and three culture holes are fixedly mounted in the strip-shaped groove. The dish lid includes two sliding plates, and each sliding plate has a slider assembly on both sides that slides and engages with the sliding groove. One sliding plate has a groove on its connecting surface, and the other sliding plate has a corresponding locking block on its connecting surface. The locking block and the groove can be detachably engaged.

[0008] The method of using the embryo thawing culture dish is as follows:

[0009] The embryo thawing solutions used here are divided into solutions A, B, C, and D. To use, slide the two sliding plates of the dish lid open to the sides. After opening the plates, remove the vibratory dish from the vibratory zone. Add solutions A, B, C, and D to the vibratory dish and the three culture wells respectively. Then, cover the vibratory dish and the short groove with mineral oil. The mineral oil in the short groove will flow and completely cover the surface of the culture wells. This completes one thawing culture dish. Next, place the vibratory dish containing solution A in a 37°C incubator in the laboratory for equilibration, and leave the three culture wells at room temperature for half an hour to equilibrate. It can only be used after more than 2 hours; when performing embryo thawing, first place the mobile dish back into the mobile area to fix it, then take the carrier rod containing the embryo out of the liquid nitrogen tank and place it in solution A. At this time, the embryo will detach from the carrier rod into solution A. After placing it in solution A for 1 minute, transfer the embryo to solution B, place it for 3 minutes, then transfer it to solution C, place it for 5 minutes, then transfer it to solution D, place it for 5 minutes. At this time, the embryo will be revive and alive. The thawing process is now complete. The specific details of the embryo transfer operation and the placement time in each solution should be carried out according to the operating instructions of the thawing solution used.

[0010] Furthermore, a pair of positioning blocks are symmetrically arranged in the sliding area; a notch corresponding to the positioning blocks is provided on the outer side of the movable dish. Through the cooperation of the notch and the positioning block, the movable dish and the dish body can be accurately and stably installed in the movable area of ​​the dish body, which is simple and convenient to operate and has a good connection effect.

[0011] Furthermore, the sliding groove includes a strip groove and a movable groove. The strip groove is located on the outside of the dish body; the movable groove is located in the dish body inside the strip groove, and neither end of the movable groove is connected to the outside. A strip hole is provided between the strip groove and the movable groove for communication. The slider assembly includes a strip block and a T-shaped limiting block. The strip block is slidably disposed in the strip groove, and the T-shaped limiting block is slidably disposed in the movable groove. The T-shaped limiting block is made of silicone. When installing the sliding plate and the dish body, the T-shaped limiting block can be bent and deformed to fit into the movable groove. During use, the strip block and the strip groove cooperate, and the T-shaped limiting block slides through the strip hole and is installed in the movable groove. Because neither end of the movable groove is connected to the outside, it can prevent the sliding plate from detaching from the dish body, thus avoiding the problem of incorrect capping. The way the strip block and the strip groove cooperate, and the T-shaped limiting block slides through the strip hole and is installed in the movable groove, allows the sliding plate to slide stably on the dish body without detaching, effectively avoiding the risk of medical accidents caused by dish cap separation or incorrect capping, while improving the safety and convenience of operation. The design that neither end of the movable groove is connected to the outside ensures that the sliding plate will not accidentally detach from the dish body, enhancing the overall structural stability.

[0012] Furthermore, silicone pads are provided at the contact points between the top surface of the dish and the sliding plate. During use, the elastic deformation of the silicone pads ensures a tight fit between the sliding plate and the dish, making the lid slide more smoothly and with a good seal. The silicone pads have good elasticity and friction, effectively preventing the sliding plate from shaking or misaligning during movement, and also acting as a buffer to reduce hard friction between the sliding plate and the dish.

[0013] Furthermore, a fixing block is provided on the inner side of the sliding plate that contacts the dish body; a connecting groove is provided on the dish body at the position corresponding to the fixing block, and the fixing block is connected to the connecting groove. Through the cooperation of the fixing block and the connecting groove, the sliding plate and the dish body are stably connected together, ensuring that the dish lid is firmly fixed in the closed state, and is not easy to be misaligned or accidentally opened, effectively improving the connection stability between the dish lid and the dish body.

[0014] Furthermore, the sliding plate has several strip-shaped notches on both sides. These notches provide an anti-slip effect and facilitate the movement of the sliding plate.

[0015] Furthermore, both the movable dish and the culture well have a diameter of 1.3 cm and a height of 0.4 cm. This size design ensures that the movable dish and culture well have a uniform standard during use, facilitating the transfer of embryos between different liquids during manipulation, while also adapting to the space requirements of conventional micromanipulation equipment and constant temperature incubators, making it convenient to use.

[0016] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0017] 1. This utility model provides an embryo thawing culture dish. By setting a sliding lid structure, the risk of confusion caused by lid separation is avoided, and the operation safety is improved. The design of the movable dish and the strip groove realizes the independent preheating and use of liquids with different temperature requirements. The strip groove makes it easy to add mineral oil to three culture wells at the same time, simplifying the operation process and improving the thawing effect. The overall structure is stable and has the advantages of convenient operation and safety.

[0018] 2. This utility model uses the cooperation of positioning blocks and notches to ensure that the movable dish is accurately and stably installed in the movable area; the slider assembly cooperates with the three-layer sliding groove to ensure that the dish lid slides smoothly and does not come off, avoiding the risk of incorrect lid placement; the silicone pad enhances sealing and sliding stability; the fixing block cooperates with the connecting groove to improve the firmness of the dish lid closure; the strip notch on the sliding plate increases the anti-slip effect and facilitates operation; the movable dish and culture well with uniform size design improve the consistency and adaptability of operation. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0020] Figure 2 This is a cross-sectional schematic diagram showing the connection relationship between the sliding plate and the dish body of this utility model.

[0021] Figure 3 This is an enlarged schematic diagram of the connection between the sliding component and the sliding groove of this utility model.

[0022] Figure 4 This is a schematic diagram showing the connection relationship between the sliding plate and the side of the dish body in this utility model.

[0023] Figure 5 This is a schematic diagram of the three-dimensional structure of the vessel body of this utility model.

[0024] Figure 6 This is a three-dimensional structural diagram of the sliding plate of this utility model.

[0025] Attached image labels:

[0026] Dishe body—1, sliding groove—11, strip groove—111, movable groove—112, strip hole—113, movable area—12, strip short groove—13, culture hole—14, positioning block—15, connecting groove—16, sliding plate—2, slider assembly—21, strip block—211, T-shaped limit block—212, groove—22, locking block—23, fixing block—24, strip notch—25, movable dish—3. Detailed Implementation

[0027] The present invention will be further described below with reference to the accompanying drawings.

[0028] Example 1: An embryo thawing culture dish includes a dish body 1. The dish body 1 has a pair of symmetrical sliding grooves 11 on its outer side, an active area 12 and a strip-shaped short groove 13 inside, and a dish lid that can be slidably mounted on the top. The active area 12 has a detachable movable dish 3, and the strip-shaped short groove 13 has three culture holes 14 fixedly mounted inside. The dish lid includes two sliding plates 2. Each sliding plate 2 has a slider assembly 21 on both sides that slides and engages with the sliding groove 11. The connecting surface of one sliding plate 2 has a groove 22, and the connecting surface of the other sliding plate 2 has a corresponding locking block 23 that engages with the groove 22. The locking block 23 and the groove 22 can be detachably engaged.

[0029] The embryo thawing solution used here is divided into solution A, solution B, solution C, and solution D. To use, slide the two sliding plates 2 of the dish lid open to the sides. After opening the sliding plates 2, remove the movable dish 3 (movable area 12). Add solutions A, B, C, and D to the movable dish 3 and the three culture wells 14 respectively. Then, cover the movable dish 3 and the short, narrow groove 13 with mineral oil. The mineral oil in the short, narrow groove 13 will flow and completely cover the surface of the culture wells 14. This completes the preparation of one thawing culture dish. Then, place the movable dish 3 containing solution A into... The embryos are equilibrated in a 37°C constant temperature incubator in the laboratory. The three culture wells 14 should be equilibrated at room temperature for at least half an hour before use. When thawing the embryos, first place the mobile dish 3 back into the mobile area 12 to fix it. Then, take the carrier rod containing the embryos out of the liquid nitrogen tank and place it in solution A. At this time, the embryos will detach from the carrier rod into solution A. After placing it in solution A for 1 minute, transfer the embryos to solution B. After placing it in solution B for 3 minutes, transfer it to solution C. After placing it in solution C for 5 minutes, transfer it to solution D for 5 minutes. At this time, the embryos will be revived and alive. The thawing process is now complete.

[0030] Example 2: Unlike Example 1, the sliding area is symmetrically provided with a pair of positioning blocks 15; the outer side of the movable dish 3 is provided with a notch that cooperates with the positioning blocks 15. Through the cooperation of the notch and the positioning blocks 15, the movable dish 3 and the dish body 1 can be accurately and stably installed in the movable area 12 of the dish body 1, which is simple and convenient to operate and has a good connection effect.

[0031] Silicone pads are provided at the contact points between the top surface of the dish body 1 and the sliding plate 2. During use, the elastic deformation of the silicone pads ensures a tight fit between the sliding plate 2 and the dish body 1, making the lid slide more smoothly and with a good seal. The silicone pads have good elasticity and friction, effectively preventing the sliding plate 2 from shaking or misaligning during movement, and also acting as a buffer to reduce hard friction between the sliding plate 2 and the dish body 1.

[0032] The inner side of the sliding plate 2 that contacts the dish body 1 is also provided with a fixing block 24; the dish body 1 is provided with a connecting groove 16 corresponding to the fixing block 24, and the fixing block 24 is connected to the connecting groove 16. Through the cooperation of the fixing block 24 and the connecting groove 16, the sliding plate 2 and the dish body 1 are stably connected together, ensuring that the dish lid is firmly fixed in the closed state, and is not easy to be misaligned or accidentally opened, effectively improving the connection stability between the dish lid and the dish body 1.

[0033] The sliding plate 2 also has several strip-shaped notches 25 on both sides. The strip-shaped notches 25 on both sides of the sliding plate 2 provide an anti-slip effect and facilitate the movement of the sliding plate 2.

[0034] Example 3: The difference from Example 2 is that the sliding groove 11 includes a strip groove 111 and a movable groove 112. The strip groove 111 is located on the outside of the dish body 1; the movable groove 112 is located in the dish body 1 inside the strip groove 111, and neither end of the movable groove 112 is connected to the outside; a strip hole 113 is provided between the strip groove 111 and the movable groove 112 for communication; the slider assembly 21 includes a strip block 211 and a T-shaped limiting block 212. The strip block 211 is slidably disposed in the strip groove 111, and the T-shaped limiting block 212 is slidably disposed in the movable groove 112. The T-shaped limiting block 212 is made of silicone. When the sliding plate 2 is installed with the dish body 1, the T-shaped limiting block 212 can be bent and deformed to fit into the movable groove 112. During use, the strip block 211 cooperates with the strip groove 111, and the T-shaped limiting block 212 slides through the strip hole 113 and is installed in the movable groove 112. Since neither end of the movable groove 112 is connected to the outside, the sliding plate 2 is prevented from detaching from the dish body 1, thus avoiding the problem of incorrect capping. The way the strip block 211 cooperates with the strip groove 111 and the T-shaped limiting block 212 slides through the strip hole 113 and is installed in the movable groove 112 allows the sliding plate 2 to slide stably on the dish body 1 without detaching, effectively avoiding the risk of medical accidents caused by the separation or incorrect capping of the dish, while improving the safety and convenience of operation. The design that neither end of the movable groove 112 is connected to the outside ensures that the sliding plate 2 will not accidentally detach from the dish body 1, enhancing the overall structural stability.

[0035] The movable dish 3 and the culture well 14 both have a diameter of 1.3 cm and a height of 0.4 cm. This size design ensures that the movable dish 3 and the culture well 14 have a uniform standard size during use, which facilitates the transfer of embryos between different liquids during manipulation, and is also compatible with the space requirements of conventional micromanipulation equipment and constant temperature incubators, making it easy to use.

[0036] Numerous specific details are set forth in this specification. However, it will be understood that embodiments of this invention may be practiced without these specific details. In some instances, well-known methods, structures, and techniques have not been shown in detail so as not to obscure the understanding of this specification.

[0037] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. An embryo thawing culture dish, characterized in that: The device includes a dish body (1), with a pair of sliding grooves (11) symmetrically arranged on the outside of the dish body (1), an active area (12) and a strip-shaped groove (13) inside, and a dish cover that can be slidably arranged on the top; an active dish (3) is detachably arranged in the active area (12), and three culture holes (14) are fixedly arranged in the strip-shaped groove (13); the dish cover includes two sliding plates (2), and each sliding plate (2) has a slider assembly (21) on both sides that slides and engages with the sliding groove (11). The connecting surface of one sliding plate (2) has a groove (22), and the connecting surface of the other sliding plate (2) has a corresponding locking block (23) for the groove (22). The locking block (23) and the groove (22) can be detachably engaged.

2. The embryo thawing culture dish as described in claim 1, characterized in that: The active area (12) is symmetrically provided with a pair of positioning blocks (15); the outer side of the active dish (3) is provided with a notch that cooperates with the positioning blocks (15).

3. The embryo thawing culture dish as described in claim 1, characterized in that: The sliding groove (11) includes a strip groove (111) and a movable groove (112). The strip groove (111) is located on the outside of the dish body (1). The movable groove (112) is located in the dish body (1) inside the strip groove (111). Neither end of the movable groove (112) is connected to the outside. A strip hole (113) is provided between the strip groove (111) and the movable groove (112) for communication. The slider assembly (21) includes a strip block (211) and a T-shaped limiting block (212). The strip block (211) is slidably disposed in the strip groove (111), and the T-shaped limiting block (212) is slidably disposed in the movable groove (112).

4. The embryo thawing culture dish as described in claim 3, characterized in that: Silicone pads are provided at the positions where the top surface of the dish (1) contacts the sliding plate (2).

5. An embryo thawing culture dish as described in any one of claims 1-4, characterized in that: The inner side of the sliding plate (2) that contacts the dish body (1) is also provided with a fixing block (24); the dish body (1) is provided with a connecting groove (16) at the position corresponding to the fixing block (24), and the fixing block (24) is connected to the connecting groove (16).

6. The embryo thawing culture dish as described in claim 1, characterized in that: The sliding plate (2) also has several strip-shaped notches (25) on both sides.

7. The embryo thawing culture dish as described in claim 1, characterized in that: The diameter of the active dish (3) and the culture well (14) are both 1.3 cm and the height is both 0.4 cm.

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