Microfluidic device for stepwise PCR reaction

Abstract

The utility model relates to PCR reaction device technical field especially for a kind of microfluidic step-by-step PCR reaction device, including tube body, taper, connecting body, capillary conduit, solid pipe seat, top plate, connecting piece and pipe cover;The utility model is connected by using capillary conduit with two reaction tubes, after the first step PCR reaction is completed, the product part of the first step PCR reaction can be transferred to the second step PCR reaction tube under the condition that reaction tube is not opened, the transfer process of sample between two-step PCR reaction is simplified, reaction tube cover does not need to be opened, it can be completed in the device, to avoid the pollution possibly brought by artificial intervention, with good practicability, and the external shape size of the device is consistent with ordinary PCR reaction tube, can be perfectly adapted to general PCR instrument on the market, reduce production cost.

Description

Technical Field

[0001] This utility model relates to the field of PCR reaction device technology, specifically to a microfluidic stepwise PCR reaction device. Background Technology

[0002] PCR technology is a technique used to amplify specific DNA fragments in vitro. Nested PCR is a two-round PCR amplification technique that plays an important role in life science research and medical diagnosis. Nested PCR technology is also used in some diagnostic reagent products.

[0003] Currently, compared to conventional one-step PCR, existing nested amplification technology is relatively complex to operate and has strict requirements for experimental conditions. The most significant challenge arises from the need to open the reaction tube after the first round of PCR and remove a portion of the product for the second round of PCR. This process requires manual intervention and is prone to contamination, thus requiring strict and cumbersome operation.

[0004] Based on this, the present invention designs a microfluidic stepwise PCR reaction device to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a microfluidic stepwise PCR reaction device.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a microfluidic stepwise PCR reaction device, comprising a tube body, a cone, a connector, a capillary tube, a fixed tube seat, a top plate, a connecting piece, and a tube cap. The bottom of the tube body is provided with a cone, and the top of the tube body is fixedly connected to the top of the tube body. A connector is fixedly connected to one side of the tube body below the top plate. The other end of the connector is fixedly connected to the outside of another tube body. A capillary tube is provided inside the connector. The capillary tube has a U-shaped structure, and the openings at both ends of the capillary tube are respectively located inside the two tube bodies.

[0007] As a preferred embodiment of this utility model, two fixed pipe seats are fixedly connected to the inner walls of adjacent sides of the two pipe bodies, and the bent portion of the capillary tube passes through the fixed pipe seats and is fixedly installed inside the fixed pipe seats.

[0008] As a preferred technical solution of this utility model, two sealing grooves are provided on the inner wall of the upper end of the pipe body.

[0009] As a preferred embodiment of this utility model, a connecting piece is fixedly connected to the rear end of the top plate, and a pipe cover is fixedly connected to the connecting piece.

[0010] As a preferred technical solution of this utility model, the pipe cover includes a cover plate, a connecting ring, a sealing ring and a convex plate. The cover plate is fixedly connected to the connecting piece. A connecting ring is fixedly connected to the center of the cover plate. Two sealing rings are provided on the outer side of the connecting ring.

[0011] As a preferred embodiment of this utility model, a protruding plate is fixedly connected to the outer side of the cover plate, and the protruding plate is an arc-shaped plate structure.

[0012] As a preferred technical solution of this utility model, the upper and lower sealing rings provided on the outer side of the connecting ring can be sealed and connected with the upper and lower sealing grooves opened on the inner wall of the pipe body.

[0013] As a preferred technical solution of this utility model, the connecting piece is made of a material with a certain degree of flexibility and can be bent.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. This utility model connects two reaction tubes by using a capillary tube. After the first PCR reaction is completed, the product of the first PCR reaction can be transferred to the second PCR reaction tube by a simple squeezing operation without opening the reaction tube. This simplifies the sample transfer process between the two PCR reactions. It can be completed within the device without opening the reaction tube cap, thus avoiding contamination that may be caused by manual intervention and has good practicality.

[0016] 2. The external shape and size of this device are consistent with ordinary PCR reaction tubes, which can be perfectly adapted to general PCR instruments on the market. Therefore, no new instruments are needed when using it, and no additional costs will be incurred. It can be used for pathogen detection, genetic testing, tumor gene testing, etc. It is especially suitable for scenarios such as multiplex respiratory pathogen detection, multiplex meningitis pathogen detection, and point-of-care testing that require stepwise PCR, and has good adaptability. Attached Figure Description

[0017] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0018] Figure 1 This is a schematic diagram of the overall front view of the present invention;

[0019] Figure 2 This is a schematic diagram of the overall side view structure of this utility model;

[0020] Figure 3 This is a schematic diagram of the overall orthographic structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the overall side section structure of this utility model.

[0022] In the figure: 1. Pipe body; 101. Sealing groove; 2. Cone; 3. Connector; 4. Capillary tube; 5. Fixed pipe seat; 6. Top plate; 7. Connecting piece; 8. Pipe cover; 801. Cover plate; 802. Connecting ring; 803. Sealing ring; 804. Protruding plate. Detailed Implementation

[0023] The following will refer to the appendix in the embodiments of this utility model. Figure 1-4 The technical solutions in the embodiments of this utility model are clearly and completely described herein. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0024] Example

[0025] Please see Figure 1-4 The present invention provides the following technical solution: a microfluidic stepwise PCR reaction device, comprising a tube body 1, a cone 2, a connector 3, a capillary tube 4, a fixed tube seat 5, a top plate 6, a connecting piece 7, and a tube cap 8. The bottom of the tube body 1 is provided with a cone 2, and the top of the tube body 1 is fixedly connected to the top of the tube body 1. The connector 3 is fixedly connected to one side of the tube body 1 below the top plate 6. The other end of the connector 3 is fixedly connected to the outside of another tube body 1. The inside of the connector 3 is provided with a capillary tube 4, which has a U-shaped structure. The openings at both ends of the capillary tube 4 are respectively located inside the two tube bodies 1.

[0026] Two fixed pipe seats 5 are fixedly connected to the inner walls of adjacent sides of the two pipe bodies 1. The bent part of the capillary tube 4 passes through the fixed pipe seat 5 and is fixedly installed inside the fixed pipe seat 5.

[0027] With the connection structure of the two reaction tubes described above, the first round of PCR reaction products can be transferred by squeezing through the capillary tube 4, which simplifies the sample transfer process between the two PCR reactions and avoids interference caused by manual transfer.

[0028] Two sealing grooves 101 are provided on the inner wall of the upper end of the pipe body 1.

[0029] A connecting piece 7 is fixedly connected to the rear end of the top plate 6, and a pipe cover 8 is fixedly connected to the connecting piece 7.

[0030] The pipe cover 8 includes a cover plate 801, a connecting ring 802, a sealing ring 803, and a protruding plate 804. The cover plate 801 is fixedly connected to the connecting piece 7. The connecting ring 802 is fixedly connected to the center of the cover plate 801. Two sealing rings 803 are provided on the outer side of the connecting ring 802.

[0031] A protruding plate 804 is fixedly connected to the outer side of the cover plate 801. The protruding plate 804 is an arc-shaped plate structure.

[0032] The upper and lower sealing rings 803 provided on the outer side of the connecting ring 802 can be sealed and connected with the upper and lower sealing grooves 101 opened on the inner wall of the pipe body 1.

[0033] The connecting piece 7 is made of a flexible material that can be bent.

[0034] The two sealing rings 803 provided on the tube cap 8 can cooperate with the two sealing grooves 101 opened on the inner wall of the upper end of the tube body 1 to form a sealing connection. This double sealing design maintains the airtightness of the reaction tube.

[0035] The working principle and usage process of this utility model are as follows: In specific use, the first step PCR reaction system is added to one of the reaction tubes. After the first round of PCR amplification, the PCR reaction product in the first reaction tube is squeezed through the capillary tube 4 into the second reaction tube connected by the connector 3, thereby completing the transfer of the PCR reaction product. The second step PCR reaction system can also be added after the first step PCR reaction is completed, so that the second round of amplification can be carried out through the PCR reaction device. Throughout the entire process of PCR reaction product transfer, the cap of the reaction tube is kept sealed. This process can avoid contamination caused by human intervention and ensure the accuracy of the detection experiment.

[0036] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A microfluidic stepwise PCR reaction device, characterized in that: The tube includes a tube body (1), a cone (2), a connector (3), a capillary tube (4), a fixed tube seat (5), a top plate (6), a connecting piece (7), and a tube cap (8). The bottom of the tube body (1) is provided with a cone (2). The top of the tube body (1) is fixedly connected to a top plate (6). The bottom of the tube body (1) is fixedly connected to one side of the tube body (1) below the top plate (6). The other end of the connector (3) is fixedly connected to the outside of another tube body (1). The inside of the connector (3) is provided with a capillary tube (4). The capillary tube (4) has a U-shaped structure. The openings at both ends of the capillary tube (4) are respectively located inside the two tube bodies (1).

2. The microfluidic stepwise PCR reaction device according to claim 1, characterized in that: Two fixed pipe seats (5) are fixedly connected to the adjacent inner walls of the two pipe bodies (1), and the bent part of the capillary tube (4) passes through the fixed pipe seat (5) and is fixedly installed inside the fixed pipe seat (5).

3. The microfluidic stepwise PCR reaction device according to claim 1, characterized in that: The pipe body (1) has two sealing grooves (101) on the inner wall of the upper end.

4. The microfluidic stepwise PCR reaction device according to claim 1, characterized in that: A connecting piece (7) is fixedly connected to the rear end of the top plate (6), and a pipe cover (8) is fixedly connected to the connecting piece (7).

5. The microfluidic stepwise PCR reaction device according to claim 1, characterized in that: The pipe cover (8) includes a cover plate (801), a connecting ring (802), a sealing ring (803) and a protruding plate (804). The cover plate (801) is fixedly connected to the connecting piece (7). The connecting ring (802) is fixedly connected to the center of the cover plate (801). Two sealing rings (803) are provided on the outer side of the connecting ring (802).

6. The microfluidic stepwise PCR reaction device according to claim 5, characterized in that: A protruding plate (804) is fixedly connected to the outer side of the cover plate (801), and the protruding plate (804) is an arc-shaped plate structure.

7. The microfluidic stepwise PCR reaction device according to claim 5, characterized in that: The upper and lower sealing rings (803) provided on the outer side of the connecting ring (802) can be sealed and connected with the upper and lower sealing grooves (101) opened on the inner wall of the pipe body (1).

8. The microfluidic stepwise PCR reaction device according to claim 1, characterized in that: The connecting piece (7) is made of a flexible material that can be bent.

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