Water quality microorganism detection culture device with precise temperature control

Abstract

The utility model provides a kind of water quality microbiological detection culture device of precision temperature control, it is related to microbiological culture technical field, including temperature control box, the inside of temperature control box is provided with carrier box tray, the inside of carrier box tray is provided with six incubator, the outside of temperature control box is provided with oxygen regulation mechanism, oxygen regulation mechanism includes booster air pump, the output end of booster air pump is fixedly connected with gas pipe.The water quality microbiological detection culture device of precision temperature control, by the mutual cooperation of temperature control box, carrier box tray, incubator, sealing perspective door, sealing ring, oxygen sensor, connecting pipe, airtight solenoid valve, booster air pump, gas pipe, air pipe, gas pipe and button controller, each incubator can store appropriate water body sample with microorganism, and then when the batch of microorganism needs aerobic culture, operate button controller to control both airtight solenoid valves to be electrified and be communicated, and control booster air pump to be electrified and start pumping.

Description

Technical Field

[0001] This utility model relates to a water quality microbial culture device, specifically a water quality microbial detection and culture device with precise temperature control, belonging to the field of microbial culture technology. Background Technology

[0002] Microbial culture is the process of allowing microorganisms to grow and reproduce under artificially controlled conditions such as nutrition and temperature until they reach a detectable state. In water quality microbial testing, when it is necessary to count the number of target bacteria, observe characteristics to identify species, use specific temperatures to screen target bacteria, or evaluate the activity of functional microorganisms, appropriate culture devices are required. These devices provide a stable environment for testing.

[0003] A utility model patent with authorization announcement number CN222007747U discloses a microbial culture device. By setting up a turntable, a placement tray, a sampling tube, an electric push rod, and a lifting assembly, when sampling and observing microorganisms in the incubator, the placement tray on the turntable is moved to below the sampling tube by a motor controlled by a motor. As the sampling tube moves downward driven by the electric push rod, the lifting assembly can push the placement tray upward. When the sealing plug on the sampling tube is opened, the culture dish on the placement tray at the designated position can be taken out. Compared with the prior art of taking out the culture dish by opening the chamber door, this avoids the impact of temperature and humidity changes in the incubator on microbial culture and improves the effect of microbial culture.

[0004] While the above-mentioned technical solution allows for the individual removal of culture dishes from designated locations, improving microbial culture to some extent, it fails to consider the aerobic and anaerobic microorganisms present in different water qualities. It also lacks an oxygen regulation structure to control the oxygen content in the culture environment, hindering the targeted cultivation of different water microorganisms and limiting the application scenarios of the cultivation device. Therefore, this paper proposes a water quality microbial detection and cultivation device with precise temperature control. Utility Model Content

[0005] This invention proposes a water quality microbial detection and culture device with precise temperature control, which solves the problem that the existing technology cannot regulate the oxygen content in the culture environment, which is not conducive to the targeted culture of different types of water microorganisms, thus limiting the application scenarios of the culture device.

[0006] This utility model is achieved through the following technical solution: a water quality microbial detection and culture device with precise temperature control, including a temperature control chamber, a carrier tray inside the temperature control chamber, six culture boxes inside the carrier tray, and an oxygen regulation mechanism on the outside of the temperature control chamber;

[0007] The oxygen control mechanism includes a booster pump, the output end of which is fixedly connected to an air supply pipe, and the input end of which is fixedly connected to an air inlet pipe. A sealed transparent door is movably hinged to the left side of the temperature control box. A sealing ring is fixedly connected to the front of the temperature control box. Connecting pipes are fixedly connected to both sides of the temperature control box. Airtight solenoid valves are fixedly connected to the ends of the two connecting pipes that are far apart from each other. An oxygen sensor is fixedly connected to the inner bottom wall of the temperature control box. An air outlet pipe is fixedly connected to the output end of one of the airtight solenoid valves, and the input end of the other airtight solenoid valve is fixedly connected to the end of the air supply pipe that is far away from the booster pump. A button controller is fixedly connected to the front of the temperature control box. The temperature control box, the booster pump, the two airtight solenoid valves, and the oxygen sensor are all electrically connected to the button controller via wires.

[0008] Specifically, the bottom surface of the carrier tray is in contact with the inner bottom wall of the temperature control chamber, the outer surface of each culture box is in contact with the inner wall of the carrier tray, the bottom surface of the booster pump is fixedly connected to the upper surface of the temperature control chamber, the front of the sealing ring is in contact with the back of the sealing transparent door, and four pads are fixedly connected to the bottom surface of the temperature control chamber, with an anti-slip pad fixedly connected to the bottom surface of each pad.

[0009] Furthermore, a handle support is fixedly connected to the upper surface of the temperature control box, and a pull rod is fixedly connected to the inner wall of the handle support.

[0010] The handle support has a grip inside, and the inner wall of the grip is rotatably connected to the outer surface of the pull rod.

[0011] Preferably, a fixing rod is fixedly connected to the right side of the temperature control box, and a limit rod is fixedly connected to the right side of the sealed transparent door.

[0012] Furthermore, a limiting block is rotatably connected to the outer surface of the fixed rod, and the interior of the limiting block is engaged with the outer surface of the limiting rod.

[0013] This invention provides a water quality microbial detection and culture device with precise temperature control, which has the following beneficial effects:

[0014] This precise temperature-controlled water quality microbial detection and culture device, through the coordinated operation of a temperature control chamber, carrier tray, culture boxes, sealed transparent door, sealing ring, oxygen sensor, connecting pipe, airtight solenoid valve, booster pump, air supply pipe, air inlet pipe, air outlet pipe, and button controller, allows each culture box to hold an appropriate amount of water sample containing microorganisms. When aerobic culture of these microorganisms is required, the button controller energizes both airtight solenoid valves and activates the booster pump. When a high concentration of oxygen is needed, the air inlet pipe can be connected to an external high-concentration oxygen supply system. This creates an environment with high air and oxygen circulation within the temperature control chamber and sealed transparent door. Conversely, when a low-oxygen or even anaerobic environment is required, the air supply... The tube needs to be connected to an external supply of inert gases such as nitrogen, and the oxygen concentration range to be monitored by the oxygen sensor is preset on the button controller. Then, the button controller can control the booster pump to pump air and control the connection of two airtight solenoid valves. When the oxygen sensor detects that the oxygen concentration between the temperature control chamber and the sealed transparent door reaches the preset range of the button controller, the button controller will immediately control the two airtight solenoid valves to be de-energized and disconnected, and control the booster pump to stop pumping air. This achieves the effect of increasing the oxygen environment regulation, which expands the application scenarios of the entire culture device and avoids the problem that the lack of an oxygen regulation structure that can control the oxygen content in the culture environment is not conducive to the targeted cultivation of different types of water microorganisms, thus limiting the application scenarios of the culture device. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of a water quality microbial detection and culture device with precise temperature control according to the present invention;

[0016] Figure 2 This is a cross-sectional view of the temperature control box of this utility model;

[0017] Figure 3 This is a side view of the temperature control box of this utility model.

[0018] Figure 4 This is a bottom view of the temperature control box structure of this utility model.

[0019] Explanation of reference numerals in the attached figures

[0020] 1. Temperature control chamber; 2. Carrier tray; 3. Incubator;

[0021] 4. Oxygen control mechanism; 401. Sealed transparent door; 402. Sealing ring; 403. Oxygen sensor; 404. Connecting pipe; 405. Airtight solenoid valve; 406. Booster pump; 407. Air supply pipe; 408. Inlet pipe; 409. Outlet pipe; 410. Button controller;

[0022] 5. Pad; 6. Anti-slip pad; 7. Handle support; 8. Pull rod; 9. Grip; 10. Fixing rod; 11. Limiting rod; 12. Limiting block. Detailed Implementation

[0023] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this application.

[0024] Please see Figures 1-4 This utility model provides a precise temperature-controlled water quality microbial detection and cultivation device, including a temperature control chamber 1. The temperature control chamber 1 is made of heat-insulating material and contains a temperature sensor, heating and cooling modules, and a circulating ventilation device. Its core function is to reduce external temperature interference through the heat-insulating chamber, precisely adjust the temperature inside the chamber according to the set value, and the circulation device avoids local temperature differences. Combined with other control structures, it achieves temperature setting and monitoring, ultimately providing a stable and uniform constant temperature environment for water quality microbial cultivation, ensuring normal microbial growth and accurate detection. The temperature control chamber 1 has a carrier tray 2 inside, and six cultivation boxes 3 are arranged inside the carrier tray 2. The carrier tray 2 provides a stable and supportive structure for the cultivation boxes 3. The cultivation boxes 3 can hold an appropriate amount of water containing microorganisms. An oxygen regulation mechanism 4 is provided on the outside of the temperature control chamber 1. The oxygen regulation mechanism 4 includes a booster pump 406. The output end of the booster pump 406 is fixedly connected to an air supply pipe 407, and the input end of the booster pump 406 is fixedly connected to an air inlet pipe 408. The bottom surface of the carrier tray 2 is in contact with the inner bottom wall of the temperature control chamber 1. The outer surface of each culture box 3 is in contact with the inner wall of the carrier tray 2. The bottom surface of the booster pump 406 is fixedly connected to the upper surface of the temperature control chamber 1. Four pads 5 are fixedly connected to the bottom surface of the temperature control chamber 1. Each pad 5 has an anti-slip pad 6 fixedly connected to its bottom surface. By setting the pads 5, the temperature control chamber 1 can be raised, thereby preventing the bottom of the temperature control chamber 1 from being directly bumped or worn by the supporting structure below. By setting the anti-slip pads 6, the anti-slip properties of the bottom surface of the pads 5 can be increased.

[0025] Please refer to this carefully. Figure 1 , Figure 2 , Figure 3 and Figure 4A sealed viewing door 401 is movably hinged to the left side of the temperature control chamber 1. The sealed viewing door 401 adopts a heat-insulating structure and is inlaid with heat-insulating transparent glass, so that a part of the structure inside the temperature control chamber 1 can be observed without opening the sealed viewing door 401. A sealing ring 402 is fixedly connected to the front of the temperature control chamber 1. The sealing ring 402 can increase the airtightness of the sealed viewing door 401 and the temperature control chamber 1 after they are closed. The front of the sealing ring 402 contacts the back of the sealed viewing door 401. Connecting pipes 404 are fixedly connected to both sides of the temperature control chamber 1. A handle support 7 is fixedly connected to the upper surface of the temperature control chamber 1. A pull rod 8 is fixedly connected to the inner wall of the handle support 7. By setting the pull rod 8, a rotation base surface can be provided for the hand grip structure inside the handle support 7, thereby increasing the flexibility of the hand grip structure. By setting the handle support 7 and the pull rod 8, the entire culture device can be lifted and moved together with the above-mentioned hand grip structure.

[0026] Please refer to this carefully. Figure 1 , Figure 2 , Figure 3 and Figure 4 Two connecting pipes 404 are fixedly connected to airtight solenoid valves 405 at their far ends. The airtight solenoid valves 405 can be connected or closed by other structures under electrical control and have good airtightness. The specific model can be selected according to the application scenario. An oxygen sensor 403 is fixedly connected to the inner bottom wall of the temperature control box 1. The oxygen sensor 403 is a mature technology and is a device that converts the oxygen concentration in the gas into an electrical signal. Its main function is to monitor the oxygen content in real time and provide a basis for regulating the oxygen environment of the device. The oxygen information is converted into an electrical signal for monitoring. The specific model can be selected according to the application scenario. A handle 9 is set inside the handle support 7. The inner wall of the handle 9 is rotatably connected to the outer surface of the pull rod 8. By setting the handle 9, it can rotate around the outer surface of the pull rod 8 inside the handle support 7, and then it is convenient to lift and move the entire culture device by holding the handle 9.

[0027] Please refer to this carefully. Figure 1 , Figure 2 , Figure 3 and Figure 4 One of the airtight solenoid valves 405 has its output end fixedly connected to the air outlet pipe 409, and the other airtight solenoid valve 405 has its input end fixedly connected to the end of the air supply pipe 407 away from the booster air pump 406. A fixing rod 10 is fixedly connected to the right side of the temperature control box 1, and a limit rod 11 is fixedly connected to the right side of the sealed transparent door 401. By setting the fixing rod 10, a rotation base surface can be provided for other limit structures, and by setting the limit rod 11, the above-mentioned limit structures can be limited.

[0028] Please refer to this carefully. Figure 1 , Figure 2 , Figure 3 and Figure 4 A button controller 410 is fixedly connected to the front of the temperature control box 1. The button controller 410 can be an MCU or an Arduino series. It is small in size, low in cost and low in power consumption, and suitable for miniaturization and embedded applications. It can control multiple components through programming. It can be connected to the control terminal of each electrical component through digital output pins and control the power of each component through analog output pins. Specific control logic and timing can be implemented by writing code. The temperature control box 1, the booster air pump 406, the two airtight solenoid valves 405 and the oxygen sensor 403 are all electrically connected to the button controller 410 through wires. The outer surface of the fixed rod 10 is rotatably connected to the limit block 12. The inside of the limit block 12 is engaged with the outer surface of the limit rod 11. By setting the limit block 12, it can rotate around the outer surface of the fixed rod 10 and engage with the outer surface of the limit rod 11, thereby temporarily locking the relative position relationship between the temperature control box 1 and the sealed transparent door 401.

[0029] In use, the water sample to be cultured is first placed in the culture box 3, then the culture box 3 is placed in the carrier tray 2, and then the carrier tray 2 is placed in the temperature control chamber 1. The sealing transparent door 401 is closed, the limiting block 12 is engaged and fixed with the limiting rod 11, and the sealing ring 402 ensures that the chamber is sealed. The target temperature of the temperature control chamber 1 is set by the button controller 410, and the temperature control module built into the temperature control chamber 1 starts to work to maintain the temperature inside the chamber stable and meet the temperature requirements for microbial culture.

[0030] Regarding oxygen regulation, if an aerobic environment is required, the booster pump 406 is started via the button controller 410, and two airtight solenoid valves 405 are opened simultaneously. External gas enters the booster pump 406 through the inlet pipe 408, and is sent into the temperature control chamber 1 through the delivery pipe 407 and connecting pipe 404. The original gas in the chamber is discharged from the outlet pipe 409 through the connecting pipe 404 and airtight solenoid valve 405 on the other side, forming a gas circulation. If a high-oxygen environment is required, the inlet pipe 408 can be connected to an external high-oxygen source.

[0031] If a low-oxygen or anaerobic environment is required, the air inlet pipe 408 is connected to an inert gas source. The button controller 410 presets the oxygen concentration range, and the oxygen sensor 403 monitors the oxygen concentration in the chamber in real time and feeds it back to the button controller 410. When the concentration reaches the preset value, the button controller 410 closes the airtight solenoid valve 405 and the booster pump 406 to maintain a stable oxygen environment in the chamber. The internal cultivation can be observed through the sealed transparent door 401 without the need for frequent door opening, reducing environmental interference. The design of the entire precise temperature-controlled water quality microbial detection and cultivation device effectively solves the problem that the lack of an oxygen regulation structure that can control the oxygen content in the cultivation environment makes it difficult to cultivate different types of water quality microorganisms, thus limiting the application scenarios of the cultivation device.

[0032] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A water quality microbial detection and culture device with precise temperature control, comprising a temperature control chamber (1), characterized in that: The temperature control chamber (1) is provided with a carrier tray (2) inside, and six culture boxes (3) are provided inside the carrier tray (2). An oxygen regulation mechanism (4) is provided on the outside of the temperature control chamber (1). The oxygen control mechanism (4) includes a booster pump (406), the output end of which is fixedly connected to an air supply pipe (407), and the input end of which is fixedly connected to an air inlet pipe (408). A sealed viewing door (401) is hinged to the left side of the temperature control box (1). A sealing ring (402) is fixedly connected to the front of the temperature control box (1). Connecting pipes (404) are fixedly connected to both sides of the temperature control box (1). Airtight solenoid valves (405) are fixedly connected to the ends of the two connecting pipes (404) that are far apart from each other. An oxygen sensor (403) is fixedly connected to the inner bottom wall of the temperature control box (1). The output end of one of the airtight solenoid valves (405) is fixedly connected to the air outlet pipe (409). The input end of the other airtight solenoid valve (405) is fixedly connected to the end of the air supply pipe (407) away from the booster pump (406). A button controller (410) is fixedly connected to the front of the temperature control box (1). The temperature control box (1), the booster pump (406), the two airtight solenoid valves (405) and the oxygen sensor (403) are all electrically connected to the button controller (410) through wires.

2. The water quality microbial detection and culture device with precise temperature control according to claim 1, characterized in that: The bottom surface of the carrier tray (2) is in contact with the inner bottom wall of the temperature control chamber (1), the outer surface of each culture box (3) is in contact with the inner wall of the carrier tray (2), the bottom surface of the booster pump (406) is fixedly connected to the upper surface of the temperature control chamber (1), the front of the sealing ring (402) is in contact with the back of the sealing transparent door (401), and four pads (5) are fixedly connected to the bottom surface of the temperature control chamber (1), and an anti-slip pad (6) is fixedly connected to the bottom surface of each pad (5).

3. The water quality microbial detection and culture device with precise temperature control according to claim 1, characterized in that: A handle support (7) is fixedly connected to the upper surface of the temperature control box (1), and a pull rod (8) is fixedly connected to the inner wall of the handle support (7).

4. The water quality microbial detection and culture device with precise temperature control according to claim 3, characterized in that: The handle support (7) is provided with a grip (9) inside, and the inner wall of the grip (9) is rotatably connected to the outer surface of the pull rod (8).

5. The water quality microbial detection and culture device with precise temperature control according to claim 1, characterized in that: A fixing rod (10) is fixedly connected to the right side of the temperature control box (1), and a limit rod (11) is fixedly connected to the right side of the sealed transparent door (401).

6. The water quality microbial detection and culture device with precise temperature control according to claim 5, characterized in that: The outer surface of the fixed rod (10) is rotatably connected to the limiting block (12), and the interior of the limiting block (12) is engaged with the outer surface of the limiting rod (11).

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