A portable electrochemiluminescence detection device

Abstract

This utility model belongs to the technical field of electrochemical detection equipment, and provides a portable electrochemiluminescence detection device. It includes a dark box housing with a main unit compartment and a detection compartment, a photon counting unit fixedly installed in the main unit compartment, and an electrochemical pressure application unit fixedly installed in the detection compartment. A horizontal partition inside the dark box housing divides its internal space into a top main unit compartment and a bottom detection compartment. The horizontal partition has a detection through-hole connecting the main unit compartment and the detection compartment. The photon counting unit performs electrochemiluminescence detection on the sensor to be tested connected to the electrochemical pressure application unit through the detection through-hole. The portable electrochemiluminescence detection device provided by this utility model has a simple structure and is easy to use, reducing the operational difficulty and complexity of electrochemiluminescence detection, improving the efficiency of electrochemiluminescence detection, and is easy to carry and transport for on-site testing. It is highly practical and has a wide range of applications.

Description

Technical Field

[0001] This utility model belongs to the technical field of electrochemical detection equipment, and specifically relates to a convenient electrochemiluminescence detection device. Background Technology

[0002] Electrochemiluminescence (ECL) refers to the chemiluminescence phenomenon induced by an electrochemical reaction on an electrode surface. Its basic principle is to provide energy through the electrode to excite luminescent molecules in a redox reaction, which then releases photons upon returning to the ground state, thus generating a detectable light signal. ECL combines chemiluminescence and electrochemical technologies and is widely used in small molecule detection and analysis, immunoassay, gene sensing, and cell sensing. However, current ECL detection primarily relies on specialized laboratory equipment, such as super-resolution fluorescence microscopes or ultra-high-resolution objectives. This often requires specialized personnel to operate the ECL detection process, which is complex and time-consuming, significantly impacting efficiency. Furthermore, current ECL detection methods often require laboratory access, preventing real-time on-site or outdoor testing, severely hindering its efficient execution and widespread application.

[0003] Therefore, it is necessary to design a convenient electrochemiluminescence detection device that can at least solve some of the above problems and defects. Summary of the Invention

[0004] To solve the above technical problems, this utility model proposes a convenient electrochemiluminescence detection device. It has a simple structure and is easy to use, which reduces the difficulty and complexity of electrochemiluminescence detection, improves the efficiency of electrochemiluminescence detection, and is easy to carry and transport for on-site detection. It is highly practical and has a wide range of applications.

[0005] The technical solution of this utility model is:

[0006] This utility model proposes a convenient electrochemiluminescence detection device, including a dark box housing with a main unit compartment and a detection compartment, a photon counting unit fixedly installed in the main unit compartment, and an electrochemical pressure application unit fixedly installed in the detection compartment.

[0007] The dark box is equipped with a horizontal partition to divide its internal space into a host compartment at the top and a detection compartment at the bottom. The horizontal partition is provided with a detection through hole connecting the host compartment and the detection compartment. The photon counting unit performs electrochemiluminescence detection on the sensor to be detected connected to the electrochemical pressure unit through the detection through hole.

[0008] Preferably, the photon counting unit includes a counting host fixedly installed in the host compartment and a sensing probe electrically connected thereto. The sensing probe is fixedly embedded in the detection through hole and is arranged facing the detection compartment.

[0009] Preferably, the upper surface of the horizontal partition is provided with a first card limiting groove adapted to the counting host, and the bottom of the counting host is fixedly embedded in the first card limiting groove;

[0010] The dark box has a reserved interface on its side wall that connects to the main unit compartment, and the counting main unit is positioned close to the reserved interface.

[0011] Preferably, the bottom wall of the dark box is provided with a placement position mark located directly below the detection through hole, and the placement position mark is arranged facing the sensing probe.

[0012] Preferably, the electrochemical pressure unit includes a micro electrochemical workstation fixedly installed in the detection chamber and an electrode converter electrically connected thereto, wherein the electrode converter is connected to the corresponding electrode of the sensor to be detected.

[0013] Preferably, the bottom wall of the dark box is provided with a second locking groove adapted to the micro electrochemical workstation, and the micro electrochemical workstation is fixedly embedded in the second locking groove.

[0014] Preferably, the top wall of the dark box is provided with a top opening communicating with the main unit compartment and a top sliding door movably disposed at the top opening position, and the front side wall of the dark box is provided with a front opening communicating with the detection compartment and a front sliding door movably disposed at the front opening position.

[0015] This utility model has the following advantages and effects compared with the prior art:

[0016] (1) A dark box with a photon counting unit and an electrochemical pressure unit is used. The electrochemical pressure unit applies pulse pressure to the sensor to be detected to induce it to release photons and generate a detectable light signal. At the same time, the photon counting unit detects the light signal to complete the electrochemiluminescence detection. The operation steps are simple and convenient, which improves the efficiency of electrochemiluminescence detection. The dark box provides a closed and light-proof detection environment, which ensures the accuracy and reliability of the detection results.

[0017] (2) The top sliding door and the front sliding door are movable and set on the top of the dark box. The photon counting unit located in the main unit compartment can be easily adjusted by pushing and pulling the top sliding door. The sensor to be tested and the electrochemical pressure unit located in the detection chamber can be easily placed or replaced by pushing and pulling the front sliding door. The structure is simple and easy to use. It is also easy to carry and transport to realize real-time electrochemiluminescence detection on site or outdoors. It is highly practical and easy to promote and use.

[0018] (3) The placement position mark is set directly below the detection through hole and opposite to the photon counting unit's sensing probe, which makes it easy for the test personnel to accurately place the sensor to be tested in the corresponding position directly below the sensing probe, thereby ensuring the accuracy and reliability of the test results and reducing the difficulty and complexity of the test operation. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the portable electrochemiluminescence detection device in the embodiments of this utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the portable electrochemiluminescence detection device in this embodiment of the present invention;

[0021] Figure 3 This is a schematic diagram of the dark box in the portable electrochemiluminescence detection device in this embodiment of the present invention;

[0022] Figure 4 This is a top view of the dark box in the portable electrochemiluminescence detection device of this utility model embodiment.

[0023] Attached reference numerals: 1. Dark box housing; 11. Main unit compartment; 12. Detection compartment; 13. Reserved interface; 14. Placement position marker; 15. Second card limiting slot; 16. Top opening; 17. Front opening; 2. Horizontal partition; 21. Detection through hole; 22. First card limiting slot; 3. Counting main unit; 4. Induction probe; 5. Micro electrochemical workstation; 6. Electrode converter; 7. Top sliding door; 8. Front sliding door. Detailed Implementation

[0024] To enable those skilled in the art to better understand this utility model, it will now be further described in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the scope of this utility model.

[0025] Example:

[0026] like Figures 1-4As shown, this utility model provides a convenient electrochemiluminescence detection device, which includes a dark box 1 with a main unit compartment 11 and a detection compartment 12, a photon counting unit fixedly installed in the main unit compartment 11, and an electrochemical pressure application unit fixedly installed in the detection compartment 12. (Reference) Figure 2 As shown, the dark box 1 is specifically divided into a host compartment 11 at the top and a detection compartment 12 at the bottom by a horizontal partition 2 set inside it.

[0027] like Figure 1 As shown, the top wall of the darkroom box 1 has a top opening 16 communicating with the main unit compartment 11 and a top sliding door 7 movably disposed at the top opening 16. The front side wall of the darkroom box 1 has a front opening 17 communicating with the detection compartment 12 and a front sliding door 8 movably disposed at the front opening 17, so as to facilitate placing the sensor to be tested into the detection compartment 12 for electrochemiluminescence detection by opening and closing the front sliding door 8. It should be noted that the sensor to be tested can specifically be an electrochemical sensor, a microbial sensor, a nucleic acid sensor, or an immunosensor used in biochemical detection in the fields of biomedicine, food safety, etc., and all of them can be detected by electrochemiluminescence using the detection equipment provided in this embodiment.

[0028] Further reference Figure 2 As shown, the photon counting unit includes a counting host 3 and an electrically connected sensing probe 4. It should be noted that the sensing probe 4 and the counting host 3 are electrically connected via a connecting cable (not shown in the figure). Specifically, the counting host 3 can be a C8855-01 photon counter provided by HAMAMATSU PHOTONICS KK, and the sensing probe 4 can be a photon counting probe compatible with the aforementioned photon counter. Given that this is a mature existing technology in this field, its specific structure and principle will not be described in detail here.

[0029] Combination Figure 2 and Figure 4 As shown, the counting host 3 is fixedly installed in the host compartment 11. The upper surface of the horizontal partition 2 is provided with a first locking groove 22 that is adapted to the counting host 3. The bottom of the counting host 3 is fixedly embedded in the first locking groove 22 to prevent the counting host 3 from shaking or shifting and to improve the stability of the counting host 3.

[0030] Further reference Figure 3As shown, the side wall of the darkroom housing 1 has a reserved interface 13 that connects to the host compartment 11. The counting host 3 is positioned close to the reserved interface 13 to facilitate connection via a cable (such as a USB cable) to the photon counting unit located inside the host compartment 11 and an external data receiving and processing device (such as a PC). Specifically, the counting host 3 in the photon counting unit is located inside the host compartment 11 near the reserved interface 13, and the interface of the counting host 3 is located at the reserved interface 13. See reference [reference needed] for details. Figure 1 As shown.

[0031] like Figure 2 and Figure 4 As shown, the sensing probe 4 is fixedly installed inside the main unit compartment 11. The horizontal partition 2 is provided with a detection through hole 21 that connects the main unit compartment 11 and the detection compartment 12. The sensing probe 4 is fixedly embedded in the detection through hole 21 and the detection end of the sensing probe 4 is arranged facing the detection compartment 12 to detect and count the light signal generated by the sensor to be tested located directly below it.

[0032] Further reference Figure 1 As shown, the electrochemical pressure application unit includes a miniature electrochemical workstation 5 fixedly installed within the detection chamber 12, and an electrode converter 6 connected to the miniature electrochemical workstation 5. The electrode converter 6 is connected to the corresponding electrode of the sensor to be detected, thereby connecting the miniature electrochemical workstation 5 and the sensor to be detected through the electrode converter 6. The miniature electrochemical workstation 5 applies a pulse voltage to the sensor to be detected, causing an electrochemical reaction in the sensor to generate a detectable light signal. It should be noted that the miniature electrochemical workstation 5 can specifically be a Sensit Smart USB-type electrochemical workstation (analyzer) from PalmSens BV (Netherlands), or a Sensit BT miniature electrochemical workstation (analyzer). The electrode converter 6 can specifically be a three-electrode converter adapted to the aforementioned miniature electrochemical workstation 5, or a paper electrode, or a three-electrode clamp, etc. Given that these are mature existing technologies in the field, their specific structures and principles will not be elaborated further here. In addition, the micro electrochemical workstation 5 can be a portable electrochemical workstation with built-in lithium battery and Bluetooth function. In this case, the micro electrochemical workstation 5 can be remotely controlled to apply pulse voltage without the need for an external power supply and computer (or mobile phone), thereby ensuring the airtightness and light-blocking of the detection chamber 12.

[0033] Combination Figure 2 and Figure 3 As shown, the micro electrochemical workstation 5 is fixedly installed in the detection chamber 12. The upper end of the bottom wall of the dark box 1 is provided with a second locking groove 15 that is adapted to the micro electrochemical workstation 5. The micro electrochemical workstation 5 is fixedly embedded in the second locking groove 15 to prevent the micro electrochemical workstation 5 from shaking or shifting.

[0034] Furthermore, the upper end of the bottom wall of the dark box 1 is provided with a placement position mark 14 located directly below the detection through hole 21. The placement position mark 14 is directly opposite the detection end of the sensing probe 4 to ensure that the sensor to be tested is directly opposite the detection end of the sensing probe 4 during the detection process, thereby improving the accuracy of the detection result data. At the same time, the electrode converter 6 is located below the detection through hole 21 and is arranged facing the placement position mark 14 to facilitate the connection between the electrode converter 6 and the sensor to be tested and to ensure that the sensor to be tested is placed inside the placement position mark 14.

[0035] In practical use: Connect the counting host 3 to the external data processing receiving device via a connecting cable, open the front sliding door 8 and place the sensor to be tested (including three electrodes) at the placement position mark 14 inside the detection chamber 12, and connect it to the three electrodes of the electrode converter 6 so that the sensor to be tested is located directly below the detection through hole 21; close the front sliding door 8 to seal the inside of the detection chamber 12 and block light, turn on the micro electrochemical workstation 5 via mobile phone or computer to apply a pulse voltage to the sensor to be tested, and at the same time, the sensing probe 4 and the counting host 3 detect the light signal (released photons) generated by the sensor to be tested, and the external data processing receiving device processes and outputs the electrochemiluminescence detection results.

[0036] In summary, the portable electrochemiluminescence detection device provided by this utility model has a simple structure and is easy to use, reducing the operational difficulty and complexity of electrochemiluminescence detection, improving the efficiency of electrochemiluminescence detection, and is easy to carry and transport for on-site testing. It is highly practical and has a wide range of applications.

[0037] The above are merely preferred embodiments of the present utility model and do not limit the patent scope of the present utility model. All equivalent changes and modifications made within the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A portable electrochemiluminescence detection device, characterized in that: It includes a dark box (1) with a host compartment (11) and a detection compartment (12), a photon counting unit fixedly installed in the host compartment (11), and an electrochemical pressure unit fixedly installed in the detection compartment (12); The dark box (1) is provided with a horizontal partition (2) to divide its internal space into a host compartment (11) at the top and a detection compartment (12) at the bottom. The horizontal partition (2) is provided with a detection through hole (21) connecting the host compartment (11) and the detection compartment (12). The photon counting unit performs electrochemiluminescence detection on the sensor to be detected connected to the electrochemical pressure unit through the detection through hole (21).

2. The portable electrochemiluminescence detection device according to claim 1, characterized in that: The photon counting unit includes a counting host (3) fixedly installed in the host compartment (11) and an induction probe (4) electrically connected thereto. The induction probe (4) is fixedly embedded in the detection through hole (21) and is arranged facing the detection compartment (12).

3. The portable electrochemiluminescence detection device according to claim 2, characterized in that: The upper surface of the horizontal partition (2) is provided with a first card limiting groove (22) that is adapted to the counting host (3), and the bottom of the counting host (3) is fixedly embedded in the first card limiting groove (22); The dark box (1) has a reserved interface (13) on its side wall that communicates with the main unit compartment (11), and the counting main unit (3) is arranged close to the reserved interface (13).

4. The portable electrochemiluminescence detection device according to claim 2, characterized in that: The bottom wall of the dark box (1) is provided with a placement position mark (14) located directly below the detection through hole (21), and the placement position mark (14) is arranged opposite to the sensing probe (4).

5. The portable electrochemiluminescence detection device according to claim 1, characterized in that: The electrochemical pressure unit includes a micro electrochemical workstation (5) fixedly installed in the detection chamber (12) and an electrode converter (6) electrically connected thereto, the electrode converter (6) being connected to the electrode of the sensor to be detected.

6. The portable electrochemiluminescence detection device according to claim 5, characterized in that: The bottom wall of the dark box (1) is provided with a second card slot (15) that is compatible with the micro electrochemical workstation (5), and the micro electrochemical workstation (5) is fixedly embedded in the second card slot (15).

7. The portable electrochemiluminescence detection device according to claim 1, characterized in that: The top wall of the dark box (1) is provided with a top opening (16) that communicates with the main unit compartment (11) and a top sliding door (7) that is movably disposed at the top opening (16). The front side wall of the dark box (1) is provided with a front opening (17) that communicates with the detection compartment (12) and a front sliding door (8) that is movably disposed at the front opening (17).

Images