A device for immobilizing chromatography tubing during virus, protein purification

Abstract

The technical problem this invention aims to solve is to provide a device for fixing chromatography tubing during virus and protein purification. This device supports rapid installation and disassembly, avoiding damage to the bottle opening or tubing, while reducing operational complexity and ensuring the efficiency, safety, and reliability of the chromatography process and experimental results. The device includes a first cover plate, a second cover plate, and a connecting structure. A first fixing tube is mounted on the first cover plate, and a second fixing tube is mounted on the second cover plate. The first fixing tube and the second half-fixing tube form a clamp structure. A hollow clamping rubber stopper is mounted at the upper end of the clamp structure, and the side wall of the clamping rubber stopper has an internal and external through-hole. A nut structure is mounted at the upper end of the clamp structure. A binding structure is mounted on the side wall of the circular cover plate. The tubing is clamped and fixed by the clamping rubber stopper, and the circular cover plate is fixed to the bottle opening of the solvent storage bottle used for chromatography by the binding structure. The clamping rubber stopper effectively prevents damage to the tubing.

Description

Technical Field

[0001] This utility model relates to the field of chemical testing technology, specifically to a device for fixing chromatography tubing during virus and protein purification. Background Technology

[0002] In virus and protein purification chromatography experiments, traditional methods of fixing tubing (such as tape wrapping, universal clamping tools, etc.) are difficult to adapt to various bottle opening diameters, easily leading to tubing slippage, displacement, or poor sealing, causing problems such as liquid leakage, sample contamination, or uneven chromatographic flow rates, seriously affecting experimental accuracy and repeatability. In addition, frequent adjustments or replacements of fixing tools will prolong operation time and increase the risk of human intervention. Utility Model Content

[0003] The technical problem to be solved by this utility model is to provide a device for fixing chromatography tubing during virus and protein purification. This device supports rapid installation and disassembly, avoids damage to the bottle mouth or tubing, reduces operational complexity, and ensures the efficiency, safety and reliability of the chromatography process and experimental results.

[0004] The technical solution adopted by this utility model to solve its technical problem is as follows: a device for fixing the chromatography pipeline during virus and protein purification, including a first cover plate and a second cover plate, both of which are semi-circular, and the side walls of the first cover plate and the second cover plate are provided with a connecting structure for fixing the two into a circular cover plate.

[0005] The first cover plate is provided with a first fixing tube, and the second cover plate is provided with a second fixing tube. The cross-sections of the first fixing tube and the second fixing tube are both semi-circular.

[0006] The first fixed tube has a first external thread on its outer side wall, and the second fixed tube has a second external thread on its outer side wall. When the first fixed tube and the second half-fixed tube are connected to form a hollow tube clamp structure, the first external thread and the second external thread form a complete external thread.

[0007] The upper end of the pipe clamp structure is provided with a hollow clamping rubber plug, and the lower end of the clamping rubber plug is located inside the pipe clamp structure.

[0008] The side wall of the clamping rubber plug is provided with an opening that extends through both the inside and outside;

[0009] The upper end of the pipe clamp structure is provided with a nut structure that is compatible with the external thread;

[0010] The circular cover plate has a binding structure on its side wall, which is used to fix the circular cover plate to the mouth of the solvent storage bottle used for chromatography.

[0011] Furthermore, the connection structure includes a connecting strip;

[0012] The connecting strip is fixedly installed on the side walls of the first cover plate and the second cover plate, with both ends of the connecting strip located on the first cover plate and the second cover plate respectively and close to each other.

[0013] The connecting strip is equipped with snap buttons at both ends.

[0014] Furthermore, the clamping rubber plug includes a first rubber plug and a second rubber plug;

[0015] The first rubber stopper is cylindrical, and the second rubber stopper is frustoconical with its larger diameter end located at the bottom. The outer diameter of the larger diameter end of the second rubber stopper is larger than the outer diameter of the first rubber stopper.

[0016] The upper end of the first rubber stopper is fixedly mounted on the large-diameter end of the second rubber stopper, and their central axes coincide.

[0017] The outer diameter of the first rubber stopper is adapted to the inner diameter of the tube clamp structure.

[0018] Furthermore, the nut structure includes a first half-nut and a second half-nut;

[0019] The first half-nut and the second half-nut are connected into a complete nut by a detachable structure;

[0020] The diameter of the threaded hole of the nut decreases from bottom to top.

[0021] Furthermore, the detachable structure includes connecting strips disposed at both ends of the first half-nut;

[0022] One end of the connecting bar is movably mounted on the side wall of the first half nut via a rotating shaft;

[0023] One end of the connecting strip is provided with a round hole at the other end, and a rod is provided in the round hole;

[0024] The second half nut has insertion holes at both ends that are adapted to the insertion rod, and the insertion rod extends into the corresponding insertion hole.

[0025] Furthermore, the binding structure includes multiple vertical straps that are evenly distributed along the circumferential direction of the circular cover plate;

[0026] A first strap and a second strap are respectively provided on the outer side of the upper middle and lower middle parts of multiple vertical straps. The first strap and the second strap are respectively provided on the outer side of multiple vertical straps by multiple fixing strips.

[0027] The ends of the first and second straps are respectively provided with Velcro.

[0028] Furthermore, both the first and second straps are elastic straps.

[0029] The beneficial effects of this utility model are as follows:

[0030] 1. Insert the tubing into the clamping stopper through the opening, place the lower end of the clamping stopper inside the tubing clamp structure, then insert the nut structure into the upper end of the tubing clamp structure and tighten it, so that the clamping stopper clamps and fixes the tubing. Then, fix the circular cover plate to the mouth of the solvent storage bottle for chromatography through the binding structure to fix the tubing, effectively avoiding damage to the bottle mouth. In addition, since the tubing is clamped and fixed by the clamping stopper, damage to the tubing can be effectively avoided.

[0031] 2. The pipeline can be fixed by matching the nut structure with the external thread and the binding structure. The operation is simple and effective, which reduces the complexity of operation and ensures the efficiency, safety and reliability of the chromatography process and the experimental results. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the device for fixing chromatography tubing during virus and protein purification according to the present invention.

[0033] Figure 2 This is a structural diagram of the first cover plate, second cover plate, first fixing pipe, second fixing pipe, and connecting structure assembly described in this utility model;

[0034] Figure 3 This is a schematic diagram showing the first cover plate, second cover plate, first fixing tube, and second fixing tube of the present invention in their open states.

[0035] Figure 4 This is a schematic diagram of the nut structure and detachable structure combination described in this utility model;

[0036] Figure 5 This is a schematic diagram of the structure of the clamping rubber plug described in this utility model;

[0037] The markings in the diagram are as follows: 1. First cover plate; 2. Second cover plate; 3. First fixing tube; 4. Second fixing tube; 5. Tube clamp structure; 6. Clamping plug; 6. First plug 601; 602. Opening; 7. Connecting strap; 8. Snap fastener; 9. Nut structure; 10. First half nut 101; Second half nut 102; Connecting strip; 11. Insert rod; 12. Vertical strap; 13. First strap; 14. Second strap; 15. Fixing strip; 16. Velcro; 17. Detailed Implementation

[0038] The specific embodiments of this utility model will be further described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0039] It should be noted that all directional indicator terms such as "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" in the embodiments of this application indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. They are only used to explain the relative positional relationship and movement of the components in a specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0040] In this application, unless otherwise expressly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0041] Furthermore, if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0042] like Figure 1-5As shown, the device for fixing the chromatography tubing during virus and protein purification includes a first cover plate 1 and a second cover plate 2, both of which are semi-circular. The side walls of the first cover plate 1 and the second cover plate 2 are provided with a connecting structure for fixing the two into a circular cover plate. In addition, a silicone protective layer can be provided on the lower surface of the first cover plate 1 and the second cover plate 2 to further avoid damage to the bottle mouth.

[0043] The first cover plate 1 is provided with a first fixing tube 3, and the second cover plate 2 is provided with a second fixing tube 4. The cross-section of the first fixing tube 3 and the second fixing tube 4 are both semi-circular. It should be noted that the central axis of the first fixing tube 3 coincides with the central axis of the first cover plate 1, and the lower end face of the first fixing tube 3 is flush with the lower surface of the first cover plate 1; the central axis of the second fixing tube 4 coincides with the central axis of the second cover plate 2, and the lower end face of the second fixing tube 4 is flush with the lower surface of the second cover plate 2.

[0044] The first fixed tube 3 is provided with a first external thread on its outer side wall, and the second fixed tube 4 is provided with a second external thread on its outer side wall. When the first fixed tube 3 and the second half-fixed tube are connected to form a hollow tube clamp structure 5, the first external thread and the second external thread form a complete external thread.

[0045] The upper end of the pipe clamp structure 5 is provided with a hollow clamping rubber plug 6, and the lower end of the clamping rubber plug 6 is located inside the pipe clamp structure 5.

[0046] The clamping rubber plug 6 has an opening 7 that extends through both the inside and outside of the side wall. The opening 7 is used to place the pipeline inside the clamping rubber plug 6.

[0047] The upper end of the pipe clamp structure 5 is provided with a nut structure 10 that is compatible with the external thread. The first fixed pipe 3 and the second fixed pipe 4 are fixed together by the nut structure 10 and the connecting structure, thereby clamping the clamping rubber plug 6, so that the clamping rubber plug 6 clamps and fixes the pipeline.

[0048] The circular cover plate has a binding structure on its side wall, which is used to fix the circular cover plate to the mouth of the solvent storage bottle used for chromatography, thereby fixing the entire device.

[0049] like Figure 1 , Figure 2 As shown, in this embodiment, preferably, the connection structure includes a connecting strip 8;

[0050] The connecting strip 8 is fixedly installed on the side walls of the first cover plate 1 and the second cover plate 2, and the two ends of the connecting strip 8 are respectively located on the first cover plate 1 and the second cover plate 2 and are close to each other.

[0051] The connecting band 8 is provided with snap fasteners 9 at both ends. The two ends of the connecting band 8 are connected by snap fasteners 9, so that the first cover plate 1 and the second cover plate 2 are fixed into a circular cover plate, and the first fixing tube 3 and the second half fixing tube form a hollow tube clamp structure 5.

[0052] like Figure 5 As shown, in this embodiment, preferably, the clamping rubber plug 6 includes a first rubber plug 601 and a second rubber plug 602;

[0053] The first rubber stopper 601 is cylindrical, and the second rubber stopper 602 is frustoconical with its larger diameter end located at the bottom. The outer diameter of the larger diameter end of the second rubber stopper 602 is larger than the outer diameter of the first rubber stopper 601.

[0054] The upper end of the first rubber stopper 601 is fixedly disposed on the large-diameter end of the second rubber stopper 602 and the central axes of the two coincide.

[0055] The outer diameter of the first rubber plug 601 is matched with the inner diameter of the pipe clamp structure 5. Since the outer diameter of the large diameter end of the second rubber plug 602 is larger than the outer diameter of the first rubber plug 601, the large diameter end of the second rubber plug 602 will be tightened during the tightening and downward movement of the nut structure 10, thereby clamping the pipe with the clamping rubber plug 6.

[0056] like Figure 4 As shown, in this embodiment, preferably, the nut structure 10 includes a first half-nut 101 and a second half-nut 102;

[0057] The first half nut 101 and the second half nut 102 are connected into a complete nut by a detachable structure. The detachable structure makes it easy for the pipeline to be located between the first half nut 101 and the second half nut 102.

[0058] The diameter of the threaded hole of the nut decreases from bottom to top. Since the diameter of the threaded hole of the nut decreases from bottom to top, the large diameter end of the second rubber plug 602 can ensure that the large diameter end of the second rubber plug 602 will tighten during the tightening and downward movement of the nut structure 10, thereby causing the first rubber plug 601 to tighten. That is, the opening 7 gradually narrows, so that the first rubber plug 601 and the second rubber plug 602 clamp and fix the pipeline.

[0059] like Figure 4 As shown, in this embodiment, preferably, the detachable structure includes connecting strips 11 disposed at both ends of the first half nut 101;

[0060] One end of the connecting bar 11 is movably mounted on the side wall of the first half nut 101 via a rotating shaft;

[0061] One end of the connecting strip 11 is provided with a round hole at the other end, and a plug rod 12 is provided in the round hole;

[0062] The second half nut 102 has insertion holes at both ends that are compatible with the insertion rod 12. The insertion rod 12 extends into the corresponding insertion hole. The first half nut 101 and the second half nut 102 can be easily assembled and disassembled by the cooperation of the insertion hole and the insertion rod 12. The structure is simple and easy to operate.

[0063] like Figure 1 As shown, in this embodiment, preferably, the binding structure includes a plurality of vertical straps 13 and is evenly distributed along the circumferential direction of the circular cover plate;

[0064] A first strap 14 and a second strap 15 are respectively provided on the outer side of the upper middle and lower middle parts of the multiple vertical straps 13. The first strap 14 and the second strap 15 are respectively provided on the outer side of the multiple vertical straps 13 by multiple fixing strips 16.

[0065] The ends of the first strap 14 and the second strap 15 are respectively provided with Velcro 17. The first strap 14 and the second strap 15 are tightened and fastened to the mouth of the solvent storage bottle for chromatography by the corresponding Velcro 17, thereby fixing the entire device to the solvent storage bottle.

[0066] In this embodiment, preferably, both the first strap 14 and the second strap 15 are elastic straps. Using elastic straps can better secure the first strap 14 and the second strap 15 to the mouth of the solvent storage bottle for chromatography, preventing them from falling off.

[0067] The above are merely preferred embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. An apparatus for immobilizing chromatography tubing during virus and protein purification, characterized in that: It includes a first cover plate (1) and a second cover plate (2), both of which are semi-circular. The side walls of the first cover plate (1) and the second cover plate (2) are provided with a connecting structure for fixing the two into a circular cover plate. The first cover plate (1) is provided with a first fixing tube (3), and the second cover plate (2) is provided with a second fixing tube (4). The cross-sections of the first fixing tube (3) and the second fixing tube (4) are both semi-circular. The first fixed tube (3) is provided with a first external thread on its outer side wall, and the second fixed tube (4) is provided with a second external thread on its outer side wall. When the first fixed tube (3) and the second half fixed tube are connected to form a hollow tube clamp structure (5), the first external thread and the second external thread form a complete external thread. The upper end of the pipe clamp structure (5) is provided with a hollow clamping rubber plug (6), and the lower end of the clamping rubber plug (6) is located inside the pipe clamp structure (5). The side wall of the clamping rubber plug (6) is provided with an opening (7) that extends through both the inside and outside; The upper end of the pipe clamp structure (5) is provided with a nut structure (10) that is compatible with the external thread; The circular cover plate has a binding structure on its side wall, which is used to fix the circular cover plate to the mouth of the solvent storage bottle used for chromatography.

2. A device for immobilizing a chromatography line during purification of viruses or proteins according to claim 1, characterized in that: The connection structure includes a connecting strip (8); The connecting strip (8) is fixedly installed on the side walls of the first cover plate (1) and the second cover plate (2), and the two ends of the connecting strip (8) are respectively located on the first cover plate (1) and the second cover plate (2) and are close to each other. The connecting strip (8) is provided with snap buttons (9) at both ends.

3. A device for immobilizing a chromatography column in the purification of viruses or proteins according to claim 1, characterized in that: The clamping rubber plug (6) includes a first rubber plug (601) and a second rubber plug (602); The first rubber stopper (601) is cylindrical, and the second rubber stopper (602) is frustum-shaped with its larger diameter end located at the bottom. The outer diameter of the larger diameter end of the second rubber stopper (602) is larger than the outer diameter of the first rubber stopper (601). The upper end of the first rubber stopper (601) is fixedly disposed on the large-diameter end of the second rubber stopper (602) and the central axes of the two coincide; The outer diameter of the first rubber stopper (601) is adapted to the inner diameter of the tube clamp structure (5).

4. The device for immobilizing a chromatography line during purification of viruses or proteins according to claim 1, characterized in that: The nut structure (10) includes a first half nut (101) and a second half nut (102); The first half nut (101) and the second half nut (102) are connected into a complete nut by a detachable structure; The diameter of the threaded hole of the nut decreases from bottom to top.

5. A device for immobilizing a chromatography column in the purification of viruses or proteins according to claim 4, characterized in that: The detachable structure includes connecting strips (11) disposed at both ends of the first half nut (101); One end of the connecting bar (11) is movably mounted on the side wall of the first half nut (101) via a rotating shaft; One end of the connecting strip (11) is provided with a round hole at the other end, and a plug rod (12) is provided in the round hole; The second half nut (102) has insertion holes at both ends that are adapted to the insertion rod (12), and the insertion rod (12) extends into the corresponding insertion hole.

6. The apparatus for immobilizing chromatography tubing during virus and protein purification according to claim 1, characterized in that: The binding structure includes multiple vertical straps (13) and is evenly distributed along the circumferential direction of the circular cover plate; A first strap (14) and a second strap (15) are respectively provided on the outer side of the upper middle and lower middle parts of the multiple vertical straps (13). The first strap (14) and the second strap (15) are respectively provided on the outer side of the multiple vertical straps (13) by multiple fixing strips (16). The ends of the first strap (14) and the second strap (15) are respectively provided with Velcro (17).

7. A device for immobilizing a chromatography column in the purification of viruses or proteins according to claim 6, characterized in that: Both the first strap (14) and the second strap (15) are elastic straps.

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