A cell suction buffer device

Abstract

The utility model relates to a cell sucking buffer device, including mounting bracket, the mounting bracket be provided with cross roller guide, the movable end of cross roller guide be connected with the sliding block, the sliding block be connected with the sucking needle subassembly, the upper portion of sliding block be provided with pressure sensor, the top of sliding block with pressure sensor between be provided with spring, the sliding block drive sucking needle subassembly to move up and down in vertical direction, the pressure sensor real -time detection acts on the pressure of sucking needle subassembly's needle head, the spring realizes the offset to the sliding block self -weight when sliding block removes, the utility model solves the problem that the traditional structure needle head is easy to damage and cannot very accurate thorough cultivation dish bottom, can real -time detection needle head end's resistance, has effectively reduced the probability that needle head is damaged, has improved the convenient degree of replacing needle head.

Description

Technical Field

[0001] This utility model relates to the field of visual inspection equipment technology, and in particular to a cell absorption buffer device. Background Technology

[0002] Cell aspiration devices are mainly used for cell culture, transfer, and detection. Existing such devices typically use servo motors to precisely drive the Z-axis movement, enabling the aspiration needle to descend vertically to a target position at a preset fixed height, such as near the bottom of a culture dish, for aspiration.

[0003] However, due to the inconsistent height of the bottom of the petri dishes, there may be manufacturing tolerances exceeding ±0.5mm, while the needle needs to be as close to the bottom of the petri dish as possible. The direct consequence of using a fixed height mode is that when the needle descends to the preset position, it is highly susceptible to violent collisions because the actual bottom of the petri dish is higher than expected. Such collisions can cause the delicate aspiration needle to bend or even break, resulting not only in expensive consumable losses but also in experimental interruptions, sample contamination, or data loss, leading to significant costs.

[0004] To address this collision problem, existing technologies mainly employ two improvement approaches:

[0005] 1. Improving the rigidity of the mechanical structure: This involves reinforcing the Z-axis guide rail, support structure, etc., to make it less prone to deformation or damage in the event of a collision. However, this increases the complexity and cost of the equipment, and there are physical limits to the improvement of rigidity.

[0006] 2. Improve positioning accuracy: Try using higher precision servo motors, encoders, or more sophisticated transmission mechanisms to more accurately reach the preset position. However, this method still fails to fundamentally solve the core problem of the mismatch between the "preset position" and the "actual bottom position".

[0007] More importantly, both of these improvement schemes encountered insurmountable physical bottlenecks in practice:

[0008] 1. Equipment weight (typically >5kg): The Z-axis drive system itself (including the motor, slider, needle clamp, etc.) has a large mass. According to Newton's laws of motion (F=ma), when rapid stopping (high deceleration) is required to avoid collision, a huge inertial force will act on the mechanical structure. Even with high rigidity, the huge impact force generated by sudden stopping may directly damage the needle or precision components.

[0009] 2. Sliding friction resistance: Unavoidable static and dynamic friction exists in Z-axis moving parts (such as between the slider and the guide rail). This friction has nonlinear and hysteretic characteristics, severely interfering with the sensitivity and response speed of micron-level position control. The system struggles to accurately start, stop, or make minute position corrections within extremely short distances (micron scale) to avoid suddenly appearing obstacles (such as the bottom of a dish).

[0010] Therefore, existing technologies, limited by the enormous inertia caused by the system's own weight and the interference of sliding friction, still cannot achieve truly effective micron-level real-time collision avoidance control when encountering uncertain heights in the culture dish. The risk of the needle striking the bottom of the dish has not been fundamentally resolved, which has become a key technical bottleneck restricting the reliability, automation level, and operational safety of such devices. Utility Model Content

[0011] The technical problem to be solved by this utility model is to provide a cell aspiration buffer device to solve the problem of needle bending or damage caused by large positional errors when aspirating cell culture medium, and at the same time, to enable quick needle replacement.

[0012] The technical solution adopted by this utility model to solve its technical problem is as follows: a cell aspiration buffer device, including a mounting frame, on which a cross roller guide is provided, and a slider is connected to the movable end of the cross roller guide. The slider is connected to a needle aspiration assembly. A pressure sensor is provided above the slider, and a spring is provided between the top of the slider and the pressure sensor. The slider drives the needle aspiration assembly to move up and down in the vertical direction. The pressure sensor detects the pressure acting on the needle tip of the needle aspiration assembly in real time, and the spring counteracts the weight of the slider when it moves.

[0013] Furthermore, the mounting bracket of this utility model is also provided with a light source assembly, which is a ring light source, and the needle suction assembly is located at the center of the ring light source.

[0014] Furthermore, the slider of this utility model is provided with a trigger plate on its side, and a limit photoelectric switch is provided on the mounting bracket, with the trigger plate located in the U-shaped groove of the limit photoelectric switch.

[0015] Furthermore, the trigger piece described in this utility model is L-shaped.

[0016] Furthermore, the suction needle assembly described in this utility model has a quick-release structure.

[0017] The beneficial effects of this utility model are that it solves the defects existing in the background technology. By using a combination of key components such as cross roller guides, springs, and pressure sensors, when the needle encounters resistance during the Z-axis descent, the pressure sensor determines that the needle has reached the bottom of the culture dish based on the feedback resistance value and sends a feedback signal to control the needle to stop descending. This solves the problems of easy needle damage and inability to accurately reach the bottom of the culture dish in traditional structures. It also simplifies the structural design. Because it can detect the resistance at the tip of the needle in real time, it increases the working efficiency of the equipment, effectively reduces the probability of needle damage, meets the relevant process requirements of customers, and improves the convenience of needle replacement. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of this utility model. Figure 1 ;

[0019] Figure 2 This is a schematic diagram of the structure of this utility model. Figure 2 ;

[0020] In the diagram: 1. Mounting bracket; 2. Cross roller guide; 3. Slider; 4. Pin suction assembly; 5. Pressure sensor; 6. Spring; 7. Light source assembly; 8. Trigger plate; 9. Limit photoelectric switch. Detailed Implementation

[0021] The present invention will now be described in further detail with reference to the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams, illustrating only the basic structure of the present invention, and therefore only show the components relevant to the present invention.

[0022] like Figures 1-2 The cell aspiration buffer device shown consists of a cross roller guide 2, a pressure sensor 5, a spring 6, a limit photoelectric switch 9, and a needle aspiration assembly 4. The cross roller guide 2 provides low-damping and high-precision guidance for the entire device. The spring 6 installed on the slider 3 counteracts the influence of the slider 3's own weight. The pressure sensor 5 installed on the top of the slider 3 detects the pressure acting on the end needle in real time. When a large pressure value is detected, a pulse signal is sent to stop the operation, thereby protecting the needle from damage while reaching a relatively accurate height.

[0023] The specific structure includes a mounting bracket 1, on which a cross roller guide 2 is mounted. A slider 3 is connected to the movable end of the cross roller guide 2, and the slider 3 is connected to the needle suction assembly 4. A pressure sensor 5 is mounted above the slider 3, and a spring 6 is mounted between the top of the slider 3 and the pressure sensor 5. When the slider 3 is subjected to an external force in the vertical direction, it drives the needle suction assembly 4 to move up and down. The pressure sensor 5 detects the pressure acting on the needle tip of the needle suction assembly 4 in real time, and the spring 6 counteracts the weight of the slider 3 when the slider 3 moves.

[0024] Mounting bracket 1 is also equipped with a light source assembly 7, which is a ring light source, and the needle suction assembly 4 is located at the center of the ring light source. The light source assembly 7 provides uniform ring illumination to ensure that the needle suction working area is well lit and free of shadows, while also assisting in visual positioning / observation of the needle contact or suction process.

[0025] A trigger plate 8, which is L-shaped, is provided on the side of the slider 3; a limit photoelectric switch 9 is provided on the mounting bracket 1, and the trigger plate 8 is located in the U-shaped groove of the limit photoelectric switch 9. The L-shaped plate ensures reliable alignment and blocking with the U-shaped groove photoelectric switch, improving the accuracy and repeatability of position detection.

[0026] The pressure sensor 5 detects the contact force in real time to determine whether the needle tip gently touches the bottom of the culture dish, replacing the traditional fixed-point descent and improving adaptability to different bottom heights of culture dishes; the combination of pressure sensor 5, spring 6, and cross roller guide 2 achieves high sensitivity and high responsiveness buffering, effectively protecting the needle from impact damage; the needle suction assembly 4 has a quick-release structure, which can quickly and easily replace the needle, improving operational efficiency.

[0027] The steps for aspirating cells are as follows:

[0028] 1. Use a camera to take a picture and guide the needle to move accurately above the petri dish.

[0029] 2. The needle descends vertically along the petri dish.

[0030] 3. The pressure sensor detects that the needle stops descending after the tip of the needle contacts the bottom of the culture dish by measuring the fluctuation of the pressure value.

[0031] 4. The needle tip retracts 0.02mm to avoid obstruction.

[0032] 5. The needle begins to draw blood.

[0033] 6. The needle returns to its initial waiting height to complete the procedure.

[0034] 7. When the needle needs to be replaced, it can be quickly replaced through the quick-change structure on the outside.

[0035] The above description is only a specific embodiment of the present utility model. Various examples and illustrations do not constitute a limitation on the substantive content of the present utility model. Those skilled in the art can make modifications or variations to the above-described specific embodiments after reading the description without departing from the essence and scope of the utility model.

Claims

1. A cell aspiration buffer device, comprising a mounting frame (1), characterized in that: The mounting bracket (1) is provided with a cross roller guide (2), and the movable end of the cross roller guide (2) is connected to a slider (3). The slider (3) is connected to the needle suction assembly (4). A pressure sensor (5) is provided above the slider (3), and a spring (6) is provided between the top of the slider (3) and the pressure sensor (5). The slider (3) drives the needle suction assembly (4) to move up and down in the vertical direction. The pressure sensor (5) detects the pressure acting on the needle tip of the needle suction assembly (4) in real time. The spring (6) counteracts the weight of the slider (3) when the slider (3) moves.

2. The cell aspiration buffer device as described in claim 1, characterized in that: The mounting bracket (1) is also provided with a light source assembly (7), which is a ring light source, and the needle suction assembly (4) is located at the center of the ring light source.

3. The cell aspiration buffer device as described in claim 1, characterized in that: The slider (3) is provided with a trigger piece (8) on its side, and the mounting bracket (1) is provided with a limit photoelectric switch (9). The trigger piece (8) is located in the U-shaped groove of the limit photoelectric switch (9).

4. The cell aspiration buffer device as described in claim 3, characterized in that: The trigger chip (8) is L-shaped.

5. The cell aspiration buffer device as described in claim 1, characterized in that: The suction needle assembly (4) has a quick-release structure.

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