A device for site-specific inoculation of fungi
By designing a fungal site-specific inoculation experimental device, the problems of damage and uncertain location during inoculation of living plant tissues were solved, achieving stable inoculation locations and observation effects, and improving the repeatability and accuracy of the experiment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZHEJIANG UNIV
- Filing Date
- 2025-05-29
- Publication Date
- 2026-07-10
AI Technical Summary
In existing technologies, living plant tissues are easily damaged when inoculated with mycelium cakes, the inoculation location is uncertain, resulting in high dispersion of experimental data, and the mycelium cakes are prone to displacement from living plant tissues, affecting the observation of the pathogenic process.
A fungal site-specific inoculation experimental device was designed, including a substrate, a water tank, a placement tank, and an inoculation tank. The limiting structure ensures that the relative position of the fungal cake and the living plant tissue is fixed, avoiding mechanical damage and providing a stable aquatic nutrient environment.
This reduced the dispersion of experimental data, improved experimental repeatability and observation accuracy, ensured the stability of the inoculation site, and avoided plant damage and mycelium dislocation.
Smart Images
Figure CN224478069U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of plant pathology research equipment, specifically to a fungal site inoculation experimental device. Background Technology
[0002] One important method for testing plant disease resistance is artificial inoculation. Inoculation means injecting a vaccine to prevent disease. Plants are artificially inoculated to cause disease, so that identification can be achieved. There are many types of plant diseases and different modes of transmission, so the inoculation methods are also different.
[0003] In existing technologies, plants are generally inoculated using traditional agar block inoculation or commercial inoculators. However, traditional agar block inoculation mainly relies on manual operation, and the inoculation location is highly random, resulting in high dispersion of experimental data. In large-scale screening, there is a lack of standardized positioning system, which leads to poor repeatability between different batches of experiments. On the other hand, commercial inoculators mostly use a metal needle puncture design, which can easily cause mechanical damage during use and interfere with experimental results.
[0004] In addition, after the inoculated mycelium is placed on the living plant tissue, the living plant tissue is prone to displacement, which affects the observation of the pathogenic process of the living plant tissue.
[0005] Therefore, there is a need for a fungal site inoculation experimental device that does not easily damage plants during inoculation, has the same relative position to the living plant tissue during inoculation, can reduce the dispersion of experimental data, improve the repeatability between different batches of experiments, and can prevent the fungal cake from shifting to the living plant tissue. Utility Model Content
[0006] The purpose of this invention is to provide a fungal site-specific inoculation experimental device to solve the problems in the prior art where existing plant tissues are easily damaged when inoculated with fungal cakes, and the inoculation site is not in the relative position to the plant tissue, causing the plant tissue to easily shift and affecting the observation of the pathogenic process of the plant tissue.
[0007] To achieve the above objectives, this utility model proposes a fungal site inoculation experimental device, comprising a base block, a water tank set on the base block, multiple placement slots set on the base block with one end connected to the water tank, an inoculation slot set in the placement slot, and a cover plate movably installed on the base block; a living plant tissue is placed in the placement slot, with the roots of the living plant tissue located in the water tank, and the fungal cake is placed in the inoculation slot.
[0008] Optionally, the water tank is located at the middle position of the base block, and the groove direction of the water tank is the same as the length direction of the base block.
[0009] Optionally, the placement troughs are provided in two sets and are arranged symmetrically about the water tank.
[0010] Optionally, the depth of the placement groove is less than the depth of the inoculation groove.
[0011] Optionally, the difference between the depth of the inoculation tank and the depth of the placement tank is not less than the thickness of the mycelium cake.
[0012] Optionally, multiple placement slots are equally spaced on the base block.
[0013] Optionally, a water-storing sponge is placed inside the water tank.
[0014] Optionally, the cover plate is made of a transparent material, and the cover plate and the base block are provided with a connecting structure.
[0015] Optionally, the connection structure includes a through hole in the cover plate, a rubber ring installed in the through hole, and a first limiting post on the base block corresponding to the rubber ring.
[0016] Optionally, the connection structure includes a connecting plate disposed at the bottom of the cover plate, a slider disposed on the connecting plate, a groove disposed on both sides of the base block and corresponding to the slider, and an elastic rubber block mounted on the top of the groove.
[0017] Optionally, the connection structure includes second limiting posts installed at both ends of the base block, limiting plates disposed on the second limiting posts, a first side plate disposed on the side of the cover plate, and a second side plate movably installed on another parallel side of the cover plate. The second side plate and the first side plate are provided with an insertion hole and a limiting hole. The diameter of the limiting hole is smaller than the diameter of the insertion hole. The limiting hole is oblong and communicates with the insertion hole. The distance between the insertion hole and the bottom of the base block is smaller than the distance between the limiting hole and the bottom of the base block.
[0018] Compared with the prior art, this utility model provides a fungal site inoculation experimental device, which has the following beneficial effects:
[0019] This fungal site-specific inoculation experimental device provides installation space for living plant tissue through the placement groove, which restricts the position of the living plant tissue. Combined with the inoculation groove that restricts the position of the fungal cake, the relative position of the fungal cake and the living plant tissue can be defined, ensuring the accuracy of observation of the pathogenic process of the living plant tissue. In addition, no inoculation device is required when inoculating the fungal cake, thus protecting the living plant tissue. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0021] Figure 2 This is a schematic diagram of the structure of the base block of this utility model.
[0022] Figure 3This is a top view of the base block of this utility model.
[0023] Figure 4 This is a schematic diagram of the structure of the cover plate of this utility model.
[0024] Figure 5 This is a schematic diagram of the overall structure in Embodiment 2 of this utility model.
[0025] Figure 6 This is a schematic diagram of the structure of the base block in Embodiment 2 of this utility model.
[0026] Figure 7 This is a schematic diagram of the cover plate in Embodiment 2 of this utility model.
[0027] Figure 8 This is a schematic diagram of the overall structure of Embodiment 3 of this utility model.
[0028] Figure 9 This is a schematic diagram of the structure of the base block in Embodiment 3 of this utility model.
[0029] Figure 10 This is a schematic diagram of the cover plate in Embodiment 3 of this utility model.
[0030] The diagram shows the following: 1. Base block; 2. Water tank; 21. Water storage sponge; 3. Placement tank; 4. Inoculation tank; 5. Cover plate; 6. Connecting structure; 61. Through hole; 62. Rubber ring; 63. First limiting post; 64. Connecting plate; 65. Sliding block; 66. Slide groove; 67. Elastic rubber block; 601. Second limiting post; 602. Limiting plate; 603. First side plate; 604. Second side plate; 605. Insertion hole; 606. Limiting hole. Detailed Implementation
[0031] The following detailed description, in conjunction with the accompanying drawings and specific embodiments, illustrates the present invention. Numerous specific details are set forth in the description below to provide a thorough understanding of the invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
[0032] The fungal site inoculation experimental device of this application can be used in laboratory settings for inoculating living plant tissues with pathogens, and can also be used in other similar applications. The following is a detailed description of the fungal site inoculation experimental device.
[0033] Example 1
[0034] See appendix Figure 1 — Figure 4The image shows a fungal site inoculation experimental device according to this application, including a base block 1, a water tank 2 disposed on the base block 1, a plurality of placement tanks 3 disposed on the base block 1 and connected at one end to the water tank 2, an inoculation tank 4 disposed in the placement tank 3, and a cover plate 5 movably mounted on the base block 1.
[0035] This invention utilizes a water tank 2 to provide necessary nutrient solution and water for living plant tissues; a placement tank 3 to provide space for the living plant tissues; and an inoculation tank 4 to provide space for the mycelium cake, ensuring that the relative positions of the inoculation tank 4 and the placement tank 3 remain unchanged. This guarantees that the relative positions of the mycelium cake and the living plant tissue are identical during inoculation, reducing the dispersion of experimental data and improving its accuracy. A cover plate 5 is used to seal the substrate 1, preventing the mycelium cake from moving arbitrarily and ensuring the accuracy of observation of the pathogenic process in living plant tissues.
[0036] See appendix Figure 1 — Figure 3 As shown, in this utility model, the water tank 2 is located in the middle of the base block 1, and the groove direction of the water tank 2 is the same as the length direction of the base block 1; there are two sets of placement grooves 3, which are symmetrically arranged about the water tank 2; a water storage sponge 21 is placed in the water tank 2.
[0037] This utility model provides space for the symmetrical arrangement of placement slots 3 by placing water tank 2 in the middle of base block 1 and setting the grooving direction of water tank 2 to be the same as the length direction of base block 1, thereby ensuring the number of placement slots 3. It should be noted that in this application, the grooving direction of water tank 2 is perpendicular to the grooving direction of placement slot 3, and placement slots 3 are evenly spaced on base block 1. With the setting of water storage sponge 21, the necessary water and nutrient solution can be provided to the living plant tissue for a long time.
[0038] See appendix Figure 1 — Figure 3 As shown, in this utility model, the depth of the placement groove 3 is less than the depth of the inoculation groove 4; wherein, the difference between the depth of the inoculation groove 4 and the depth of the placement groove 3 is not less than the thickness of the mycelium cake.
[0039] This invention ensures that the fungal cake can be placed by limiting the depth of the inoculation groove 4 and the placement groove 3, that is, the fungal cake can be placed at the bottom of the living plant tissue; it should be noted that the depth of the placement groove 3 is greater than the thickness of the fungal cake, thereby ensuring that the fungal cake can be placed both above and below the living plant tissue, so that the living plant tissue can be inoculated with pathogens.
[0040] See appendix Figure 1 and Figure 4As shown, in this utility model, the cover plate 5 is made of transparent material, and the cover plate 5 and the base block 1 are provided with a connecting structure 6. The connecting structure 6 includes a through hole 61 provided on the cover plate 5, a rubber ring 62 installed in the through hole 61, and a first limiting post 63 corresponding to the rubber ring 62 installed on the base block 1.
[0041] This invention ensures that staff can directly observe living plant tissues by making the cover plate 5 a transparent material; the cover plate 5 can be connected and separated from the base block 1 by the through hole 61 and the first limiting post 63; the rubber ring 62 is interference-fitted with the first limiting post 63, and the friction between the rubber ring 62 and the first limiting post 63 limits the cover plate 5, connecting the cover plate 5 to the base block 1, and preventing loosening between the through hole 61 and the first limiting post 63 after multiple disassemblies of the cover plate 5.
[0042] See appendix Figure 1 — Figure 4 As shown, the usage process of this utility model is as follows:
[0043] First, place the mycelium cake in the inoculation tank 4, then place the living plant tissue in the placement tank 3. Next, place the water-storing sponge 21, which has absorbed nutrient solution, into the water tank 2, so that it contacts the roots of the living plant tissue. Then, place the mycelium cake back into the inoculation tank 4, so that the living plant tissue is located between the two mycelium cakes. Finally, align the through hole 61 on the cover plate 5 with the first limiting post 63, and press the cover plate 5 down so that the first limiting post 63 is inserted into the through hole 61 and contacts the rubber ring 62, until the cover plate 5 can no longer move downward. It should be noted that when the cover plate 5 is installed on the clamping block 1, the bottom surface of the cover plate 5 is in contact with the mycelium cake located above the living plant tissue.
[0044] Example 2
[0045] See appendix Figure 5 — Figure 7 As shown, the difference between this embodiment and the above embodiment is that, in this embodiment, the connecting structure 6 includes a connecting plate 64 disposed at the bottom of the cover plate 5, a slider 65 disposed on the connecting plate 64, a groove 66 disposed on both sides of the base block 1 corresponding to the slider 65, and an elastic rubber block 67 installed on the top of the groove 66.
[0046] In this embodiment, the connecting plate 64 is used to connect the slider 65 to the cover plate 5. With the slider 65 and the sliding groove 66, the slider 65 can move along the sliding groove 66 to connect with the base block 1 and cover the top of the base block 1. With the elastic rubber block 67, the groove height of the sliding groove 66 is reduced, so that the sliding groove 66 and the slider 65 are interference fit, and the slider 65 is prevented from separating from the sliding groove 66 at will.
[0047] Example 3
[0048] See appendix Figure 8 — Figure 10 As shown, the difference between this embodiment and the above embodiment is that, in this embodiment, the connecting structure 6 includes a second limiting post 601 installed on both ends of the base block 1, a limiting plate 602 provided on the second limiting post 601, a first side plate 603 provided on the side of the cover plate 5, and a second side plate 604 movably installed on another parallel side of the cover plate 5 by a pin. The second side plate 604 and the first side plate 603 are provided with an insertion hole 605 and a limiting hole 606. The diameter of the limiting hole 606 is smaller than the diameter of the insertion hole 605. The limiting hole 606 is oblong and communicates with the insertion hole 605. The distance between the insertion hole 605 and the bottom of the base block 1 is smaller than the distance between the limiting hole 606 and the bottom of the base block 1.
[0049] In this embodiment, the second limiting post 601 corresponds to the limiting hole 606, and the diameter of the second limiting post 601 is less than or equal to the diameter of the limiting hole 606, allowing the second limiting post 601 to move within the limiting hole 606. The limiting plate 602 corresponds to the insertion hole 605, and the outer diameter of the limiting plate 602 is smaller than the diameter of the insertion hole 605 but larger than the diameter of the limiting hole 606. By movably connecting the second side plate 604, the second side plate 604 can rotate, allowing the first side plate... The first side plate 603 and the second side plate 604 can cooperate with the second limiting post 601 and the limiting plate 602. It should be noted that after the cover plate 5 is connected to the base block 1, the first side plate 603 and the second side plate 604 are in contact with the side wall of the base block 1. At this time, the limiting plate 602 is in contact with the outer wall of the first side plate 603 and the second side plate 604. The minimum distance between the limiting plate 602 and the side wall of the base block 1 is not less than the thickness of the first side plate 603 or the second side plate 604. The thickness of the first side plate 603 and the second side plate 604 is the same.
[0050] When installing the cover plate 5 in this embodiment, first rotate the second side plate 604 upward, then align the insertion hole 605 on the first side plate 603 with the second limiting post 601 and the limiting plate 602 and insert them, then rotate the second side plate 604 so that the second limiting post 601 and the limiting plate 602 on the other side are inserted into the insertion hole 605 on the second side plate 604; then move the cover plate 5 downward so that the second limiting post 601 enters the limiting hole 606, thus completing the installation of the cover plate 5.
[0051] The above embodiments are illustrative of this application and are not intended to limit this application. Any simple modifications to this application are within the protection scope of this application.
Claims
1. A fungal site-directed inoculation experimental apparatus, comprising a substrate (1), characterized in that, Water tank (2) set on base block (1), multiple placement slots (3) set on base block (1) and connected to water tank (2) at one end, inoculation slot (4) set in placement slot (3), and cover plate (5) movably installed on base block (1); The living plant tissue is placed in the placement trough (3), the roots of the living plant tissue are located in the water trough (2), and the mycelium cake is placed in the inoculation trough (4).
2. The fungal site-specific inoculation experimental apparatus according to claim 1, characterized in that, The water tank (2) is located in the middle of the base block (1), and the groove direction of the water tank (2) is the same as the length direction of the base block (1).
3. The fungal site-specific inoculation experimental apparatus according to claim 1, characterized in that, The placement trough (3) is provided in two sets and is symmetrically arranged about the water tank (2).
4. The fungal site-specific inoculation experimental apparatus according to claim 1, characterized in that, The depth of the placement groove (3) is less than the depth of the inoculation groove (4).
5. The fungal site-specific inoculation experimental apparatus according to claim 4, characterized in that, The difference between the depth of the inoculation tank (4) and the depth of the placement tank (3) is not less than the thickness of the mushroom cake; Multiple placement slots (3) are equally spaced on the base block (1).
6. The fungal site-specific inoculation experimental apparatus according to claim 1, characterized in that, A water-storing sponge (21) is placed inside the water tank (2).
7. The fungal site-specific inoculation experimental apparatus according to claim 1, characterized in that, The cover plate (5) is made of transparent material, and the cover plate (5) and the base block (1) are provided with a connecting structure (6).
8. The fungal site-specific inoculation experimental apparatus according to claim 7, characterized in that, The connection structure (6) includes a through hole (61) provided on the cover plate (5), a rubber ring (62) installed in the through hole (61), and a first limiting post (63) corresponding to the rubber ring (62) installed on the base block (1).
9. The fungal site-specific inoculation experimental apparatus according to claim 7, characterized in that, The connecting structure (6) includes a connecting plate (64) disposed at the bottom of the cover plate (5), a slider (65) disposed on the connecting plate (64), a groove (66) disposed on both sides of the base block (1) corresponding to the slider (65), and an elastic rubber block (67) mounted on the top of the groove (66).
10. The fungal site-specific inoculation experimental apparatus according to claim 7, characterized in that, The connection structure (6) includes a second limiting post (601) installed on both ends of the base block (1), a limiting plate (602) set on the second limiting post (601), a first side plate (603) set on the side of the cover plate (5), and a second side plate (604) movably installed on another parallel side of the cover plate (5). The second side plate (604) and the first side plate (603) are provided with an insertion hole (605) and a limiting hole (606). The diameter of the limiting hole (606) is smaller than the diameter of the insertion hole (605). The limiting hole (606) is waist-shaped and communicates with the insertion hole (605). The distance between the insertion hole (605) and the bottom of the base block (1) is smaller than the distance between the limiting hole (606) and the bottom of the base block (1).