Wall-adherent cell culture flask facilitating liquid exchange

Abstract

The utility model relates to the technical field of adherent cell culture bottle, concretely is a kind of adherent cell culture bottle convenient to change liquid, including box body subassembly, closed mechanism is installed on the box body subassembly;The lower end of the box body subassembly is equipped with connecting mechanism;The lower end middle part of the box body subassembly is equipped with first installation slot, through pipe is equipped in the inner bottom of the box body subassembly, the lower end of the through pipe extends into first installation slot, and one end of the through pipe in first installation slot is connected with discharge pipe.The application can be convenient to change liquid operation by closed mechanism, and it is also convenient to connect between bottle body, to facilitate storage and storage, and the stored liquid is discharged by discharge pipe, and liquid can be effectively discharged.

Description

Technical Field

[0001] This utility model relates to the field of adherent cell culture flask technology, and in particular to an adherent cell culture flask that is easy to change the medium. Background Technology

[0002] Cell culture refers to a method of enabling organisms to survive, grow, reproduce, and maintain their main structures and functions by simulating the in vivo environment (sterile, suitable temperature, pH, and certain nutritional conditions, etc.) outside the body. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technique. Whether for bioengineering as a whole or for biological cloning technology, cell culture is an indispensable process; cell culture itself is the large-scale cloning of cells.

[0003] When using current cell culture methods, cells need to be placed inside the culture flask. Traditionally, cells are stacked, which can cause them to slide, become misaligned, or fall out. In addition, when the culture medium needs to be changed inside the culture flask, the flask needs to be picked up and tilted, making the operation cumbersome and affecting its use.

[0004] A cell culture flask with CN222024389U, disclosed for easy medium replacement, includes a main support box. Bottom inserts are evenly inserted into the bottom surface of the main support box, and a limiting annular groove is formed on the outer side of each bottom insert. The top and bottom surfaces of the main support box have symmetrical mating holes. A discharge pipe is fixedly installed on one side of the main support box, with an end plug connected to the end of the discharge pipe away from the main support box. An extension box is obliquely fixedly installed on the side of the main support box away from the discharge pipe, with a sealing cap connected to the end of the extension box away from the main support box. A top insert is fixedly inserted into one end of each bottom insert. An oblique through hole is formed on one side of the main support box, and an internal thread is provided on the inner side of the discharge pipe. The snap-fit ​​splicing structure makes the flask more stable after assembly, and the direct discharge structure makes medium replacement more convenient.

[0005] The above-mentioned technical solution has a bottle structure that is not conducive to quick storage and retrieval, and its discharge scheme has relatively complicated operation steps that cannot be performed quickly, so improvements are needed. Utility Model Content

[0006] The purpose of this invention is to address the shortcomings of existing technologies by proposing an adherent cell culture flask that facilitates medium exchange.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] An adherent cell culture flask that facilitates medium changes includes a box assembly with a sealing mechanism installed on the box assembly; a connecting mechanism is provided at the lower end of the box assembly.

[0009] The lower center of the box assembly has a first mounting groove. A through pipe runs through the bottom of the box assembly, and the lower end of the through pipe extends into the first mounting groove. One end of the through pipe in the first mounting groove is connected to a discharge pipe. A control mechanism is provided on one side of the lower end of the box assembly. A connecting shaft is provided on the control mechanism. A connecting bracket is fixed to one end of the connecting shaft in the first mounting groove. An abutting shaft is rotatably sleeved on the connecting bracket. The abutting shaft abuts against the discharge pipe and the top of the first mounting groove.

[0010] Compared with the prior art, this application can facilitate liquid replacement operations through a closed mechanism, and also facilitates the connection between bottles for easy storage. At the same time, the stored liquid is discharged through a discharge pipe, which can fully and effectively discharge the liquid.

[0011] Preferably, the sealing mechanism includes a cap assembly threaded onto the upper end of the box assembly, and a sealing plate assembly is detachably connected to one side of the box assembly, wherein the sealing plate assembly and the box assembly are sealed together.

[0012] Furthermore, the cap assembly and sealing plate assembly can be disassembled as needed to effectively store liquids.

[0013] Preferably, the connecting mechanism includes two second notches and two first notches on both sides of the lower end of the box assembly. A positioning groove is provided on the upper end of the side of the first notch closest to the second notch. A movable plate is slidably installed in the second notch. Two positioning frames are installed on the upper end of the side of the movable plate away from the first notch. The two positioning frames located on the upper end of the same movable plate form a group. The two groups of positioning frames are respectively connected to the two positioning grooves.

[0014] Furthermore, the movable plate can be removed from the second notch and align with the first notch and positioning slot on another box assembly to complete the connection, and can be quickly disassembled, enabling effective storage.

[0015] Preferably, the control mechanism includes a second mounting groove on one side of the lower end of the box assembly, the connecting shaft is rotatably sleeved in the second mounting groove, a rotating rod is fixed to one end of the connecting shaft located in the second mounting groove, a protruding rod is installed on the rotating rod, and two positioning holes are opened on one side wall of the second mounting groove, the protruding rod and the positioning holes are corresponding.

[0016] Furthermore, by controlling the rotation of the rotating rod, the convex rod can be aligned with the corresponding positioning hole, thus effectively fixing the position of the rotating rod. And when the rotating rod can drive the connecting shaft to rotate, the abutment shaft can be controlled to rotate.

[0017] Preferably, one end of the box assembly is inclined, the bottom of the box assembly is provided with a tapered groove, and the through pipe corresponds to one end of the box assembly.

[0018] Furthermore, it facilitates rapid liquid flow, ensuring effective drainage.

[0019] The beneficial effects of this utility model are:

[0020] 1. The cap assembly and sealing plate assembly can be disassembled as needed to effectively store liquids;

[0021] 2. The movable plate can be removed from the second notch and correspond to the first notch and positioning slot on another box assembly to complete the connection, and can be quickly disassembled, which can effectively store it.

[0022] 3. By controlling the rotation of the rotating rod, the convex rod can be aligned with the corresponding positioning hole, which can effectively fix the position of the rotating rod. Furthermore, when the rotating rod can drive the connecting shaft to rotate, the rotation of the contact shaft can be controlled. Attached Figure Description

[0023] Figure 1 This is a connection structure diagram of an adherent cell culture flask that facilitates medium exchange, as proposed in this utility model.

[0024] Figure 2 This is a structural diagram of the cap assembly, sealing plate assembly, and box assembly in an adherent cell culture flask that facilitates medium exchange, as proposed in this utility model.

[0025] Figure 3 This is a cross-sectional view of an adherent cell culture flask that facilitates medium exchange, as proposed in this utility model.

[0026] Figure 4 This is a structural diagram of the first mounting groove in an adherent cell culture flask that facilitates medium replacement, as proposed in this utility model.

[0027] Figure 5 Appendix to this utility model Figure 2 Enlarged view of point A;

[0028] Figure 6 Appendix to this utility model Figure 3 Enlarged view of point B;

[0029] In the figure: 1 Cap assembly, 2 Sealing plate assembly, 3 Box assembly, 4 First mounting groove, 5 Moving groove, 6 Discharge pipe, 7 Conical groove, 8 Through pipe, 9 Connecting frame, 10 Abutting shaft, 11 Second mounting groove, 12 Protruding rod, 13 Rotating rod, 14 Connecting shaft, 15 Positioning hole, 16 Positioning groove, 17 First notch, 18 Second notch, 19 Positioning frame, 20 Moving plate. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0031] Reference Figures 1-6 An adherent cell culture flask for easy medium replacement includes a box assembly 3 with a sealing mechanism installed on it; a connecting mechanism is provided at the lower end of the box assembly 3; the sealing mechanism can be disassembled as needed to facilitate the addition of culture medium and other liquids, while the connecting mechanism allows multiple flasks to be connected to each other for easy storage and quick separation for use.

[0032] Reference Figures 1-3 The sealing mechanism includes a cap assembly 1 threaded onto the upper end of the box assembly 3. A sealing plate assembly 2 is detachably connected to one side of the box assembly 3. The sealing plate assembly 2 and the box assembly 3 are sealed together. During assembly, fasteners are provided on the sealing plate assembly 2, and locking blocks are provided on the box assembly 3. The fasteners and locking blocks correspond to each other, allowing the box assembly 3 and the sealing plate assembly 2 to abut against each other. At the same time, rubber sealing strip components are provided on opposite sides of the sealing plate assembly 2 and the box assembly 3, which can be effectively squeezed to deform them and effectively complete the sealing.

[0033] Reference Figure 1 , 2 3, 6, The connecting mechanism includes two second notches 18 and two first notches 17 on both sides of the lower end of the box assembly 3. A positioning groove 16 is provided on the upper end of the side of the first notch 17 closest to the second notch 18. A movable plate 20 is slidably installed in the second notch 18. Two positioning brackets 19 are installed on the upper end of the side of the movable plate 20 away from the first notch 17. The two positioning brackets 19 located on the upper end of the same movable plate 20 form a group. The two groups of positioning brackets 19 are respectively connected to the two positioning grooves 16. In use, the movable plate 20 is pulled out, which can make the positioning brackets 19 move out of the box assembly 3 and make the positioning brackets 19 correspond to the positioning grooves 16 on the other box assembly 3, so as to effectively complete the connection between the two box assemblies 3. At the same time, the positioning brackets 19 are made of silicone or rubber material, which can deform, making it easy for the positioning brackets 19 to be inserted into or removed from the positioning grooves 16.

[0034] ReferenceFigures 1-6 The lower middle part of the box assembly 3 has a first mounting groove 4. A through pipe 8 is provided through the bottom of the box assembly 3. The lower end of the through pipe 8 extends into the first mounting groove 4. One end of the through pipe 8 in the first mounting groove 4 is connected to a discharge pipe 6. A control mechanism is provided on one side of the lower end of the box assembly 3. A connecting shaft 14 is provided on the control mechanism. A connecting frame 9 is fixed to one end of the connecting shaft 14 in the first mounting groove 4. An abutting shaft 10 is rotatably sleeved on the connecting frame 9. The abutting shaft 10 abuts against the discharge pipe 6 and against the top of the first mounting groove 4. The rotation of the connecting shaft 14 can be controlled by the control mechanism, and the connecting shaft 14 can drive the abutting shaft 10 to rotate. As the abutting shaft 10 rotates, the abutting shaft 10 squeezes the discharge pipe 6 against the top of the first mounting groove 4 to seal it. The abutting shaft 10 and the discharge pipe 6 separate from the top of the first mounting groove 4 to facilitate discharge.

[0035] Reference Figure 2 , 4 5. The control mechanism includes a second mounting groove 11 located on one side of the lower end of the box assembly 3. A connecting shaft 14 is rotatably sleeved in the second mounting groove 11. A rotating rod 13 is fixed to one end of the connecting shaft 14 located in the second mounting groove 11. A protruding rod 12 is mounted on the rotating rod 13. Two positioning holes 15 are opened on one side wall of the second mounting groove 11, and the protruding rod 12 corresponds to the positioning holes 15. The rotating rod 13 can drive the connecting shaft 14 to rotate. As the rotating rod 13 rotates, the protruding rod 12 can be inserted into the corresponding positioning hole 15 for effective fixation. When the rotating rod 13 is in a vertical state, it can facilitate the contact shaft 10 to squeeze the discharge pipe 6 to the top of the first mounting groove 4 for sealing. When the rotating rod 13 is in a horizontal state, it can separate the contact shaft 10 from the discharge pipe 6 and separate it from the top of the first mounting groove 4 for easy discharge.

[0036] Reference Figure 3 One end of the box assembly 3 is inclined, and a conical groove 7 is provided at the bottom of the box assembly 3. The through pipe 8 corresponds to one end of the box assembly 3. When the box assembly 3 is laid flat, the liquid can be effectively discharged by flowing along the inclined side of the box assembly 3 to the through pipe 8.

[0037] In this utility model, the cap assembly 1 and the sealing plate assembly 2 can be disassembled and installed, which facilitates liquid replacement and observation. When in use, the box assembly 3 can be laid flat on the side away from the sealing plate assembly 2. After use, the control rod 13 drives the connecting shaft 14 to rotate, which can control the separation of the contact shaft 10 and the discharge pipe 6, and can separate the contact shaft 10 from the side wall of the first mounting groove 4, so that the discharge pipe 6 can be taken out to discharge the waste liquid, thereby completing the liquid replacement operation.

[0038] When storage is required, the cap assembly 1 and sealing plate assembly 2 can be connected to the box assembly 3, while the moving plate 20 is removed from the second notch 18, and the positioning frame 19 and the positioning groove 16 at the lower end of another box assembly 3 are inserted into each other to complete the connection and fixation between multiple box assemblies 3.

[0039] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An adherent cell culture flask that facilitates medium changes, comprising a housing assembly (3), characterized in that: A closing mechanism is installed on the box assembly (3); a connecting mechanism is provided at the lower end of the box assembly (3); The lower middle part of the box assembly (3) is provided with a first mounting groove (4). A through pipe (8) is provided through the bottom of the box assembly (3). The lower end of the through pipe (8) extends into the first mounting groove (4). One end of the through pipe (8) located in the first mounting groove (4) is connected to a discharge pipe (6). A control mechanism is provided on one side of the lower end of the box assembly (3). A connecting shaft (14) is provided on the control mechanism. A connecting frame (9) is fixed on one end of the connecting shaft (14) located in the first mounting groove (4). An abutting shaft (10) is rotatably sleeved on the connecting frame (9). The abutting shaft (10) abuts against the discharge pipe (6). The abutting shaft (10) abuts against the top of the first mounting groove (4).

2. The adherent cell culture flask for easy medium exchange according to claim 1, characterized in that: The sealing mechanism includes a cap assembly (1) threaded onto the upper end of the box assembly (3), and a sealing plate assembly (2) detachably connected to one side of the box assembly (3), wherein the sealing plate assembly (2) and the box assembly (3) are sealed together.

3. The adherent cell culture flask for easy medium exchange according to claim 1, characterized in that: The connecting mechanism includes two second notches (18) and two first notches (17) on both sides of the lower end of the box assembly (3). A positioning groove (16) is provided on the upper end of the side of the first notch (17) near the second notch (18). A movable plate (20) is slidably installed in the second notch (18). Two positioning frames (19) are installed on the upper end of the side of the movable plate (20) away from the first notch (17). The two positioning frames (19) located on the upper end of the same movable plate (20) form a group. The two groups of positioning frames (19) are respectively connected to the two positioning grooves (16).

4. The adherent cell culture flask for easy medium exchange according to claim 1, characterized in that: The control mechanism includes a second mounting groove (11) opened on one side of the lower end of the box assembly (3). The connecting shaft (14) is rotatably sleeved in the second mounting groove (11). A rotating rod (13) is fixed at one end of the connecting shaft (14) located in the second mounting groove (11). A protruding rod (12) is installed on the rotating rod (13). Two positioning holes (15) are opened on one side wall of the second mounting groove (11). The protruding rod (12) and the positioning holes (15) correspond to each other.

5. The adherent cell culture flask for easy medium exchange according to claim 1, characterized in that: One end of the box assembly (3) is inclined, and a tapered groove (7) is provided at the bottom of the box assembly (3). The through pipe (8) corresponds to one end of the box assembly (3).

Images